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1

Hoson, T., S. Kamisaka, M. Yamashita, and Y. Masuda. "Automorphosis of higher plants on a 3-d clinostat." Advances in Space Research 21, no. 8-9 (1998): 1229–38. http://dx.doi.org/10.1016/s0273-1177(97)00640-6.

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2

Oluwafemi, Funmilola. "Gravity Variation Effects on the Growth of Maize Shoots." Physical Sciences Forum 2, no. 1 (2021): 21. http://dx.doi.org/10.3390/ecu2021-10184.

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Gravity variation effects on plants provide definite changes. Normal Earth gravity (1G) and microgravity (µg) are possible variations for experimental purposes. On-board spaceflight microgravity experiments are rare and expensive, as the microgravity environment is an outstanding platform for research, application and education. A Clinostat was used for ground-based experiments to investigate the shoot morphology of maize plants at the Space Agency of Nigeria—National Space Research and Development Agency (NASRDA). A Clinostat device uses rotation to negate gravitational pull effects on plant
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3

Hershey, David R. "Time for a Plant Clinostat: EFFECTS OF LIGHT AND GRAVITY ON PLANTS." Science Activities: Classroom Projects and Curriculum Ideas 42, no. 1 (2005): 30–35. http://dx.doi.org/10.3200/sats.42.1.30-35.

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4

Zulkifli, Nur Athirah, Teoh Chin Chuang, Ong Keat Khim, Ummul Fahri Abdul Rauf, Norliza Abu Bakar, and Wan Md Zin Wan Yunus. "Effects of simulated microgravity on rice (MR219) growth and yield." Malaysian Journal of Fundamental and Applied Sciences 14, no. 2 (2018): 278–83. http://dx.doi.org/10.11113/mjfas.v14n2.863.

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Rice (Oryza sativa L.) is a staple food in many Asian countries with an ever increasing demand. However, the production of high quality rice seeds is insufficient to meet this demand. Research on plant growth in space related to the exposure of a microgravity environment are rare, costly and time-limited. Similar experiments can be conducted on the ground to simulate the microgravity condition using a 2-D clinostat which compensates for the unilateral influence of gravity. This study was conducted to establish a simple and cost effective technique to enhance the quality of the Malaysian rice s
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5

Shimazu, T., T. Yuda, K. Miyamoto, M. Yamashita, and J. Ueda. "Growth and development in higher plants under simulated microgravity conditions on a 3-dimensional clinostat." Advances in Space Research 27, no. 5 (2001): 995–1000. http://dx.doi.org/10.1016/s0273-1177(01)00165-x.

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6

Nhựt, Dương Tấn, Nguyễn Xuân Tuấn, Nguyễn Thị Thùy Anh, et al. "Effects of simulated microgravity on seed germination, growth, development and accumulated secondary compounds of Hibiscus sagittifolius Kurz. cultured in vitro." Vietnam Journal of Biotechnology 15, no. 1 (2018): 73–85. http://dx.doi.org/10.15625/1811-4989/15/1/12322.

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In the present study, Hibiscus sagittifolius Kurz. seeds were used as the plant materials for studying on the effects of simulated microgravity (on a 2D clinostat) on seed germination, shoot multiplication, growth, development and secondary metabolite accumulation. After surface sterilization, seeds were cultured on MS medium supplemeted with 30 g/l sucrose and 9 g/l agar in Petri dishes (9 seeds per dish, the seed to seed distance of 1.5 cm and kept in the same direction), and maintained in a Clinostat (2 rpm). The results showed that simulated microgravity inhibited the growth and developmen
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7

Kozeko, Liudmyla Y., Denis D. Buy, Yaroslav V. Pirko, Yaroslav B. Blume, and Elizaveta L. Kordyum. "Clinorotation Affects Induction of the Heat Shock Response in Arabidopsis thaliana Seedlings." Gravitational and Space Research 6, no. 1 (2020): 2–9. http://dx.doi.org/10.2478/gsr-2018-0001.

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AbstractClinorotation used to simulate microgravity effects in ground-based experiments is considered as a mild stress factor for plants. We have assumed that it might influence the plant tolerance to other stressful factors. To test this, Arabidopsis thaliana seedlings were grown on a horizontal clinostat (2 rpm) or under stationary conditions (control), and then were subjected to heat treatment. The kinetics of gene expression of cytosolic HSP70s and HSP90s during exposure to 37°C for 0.5-2 h was examined by RT-qPCR to estimate level of the heat shock reaction. It was shown that clinorotatio
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8

Rupiasih, Ni Nyoman, Ni Kadek Gita Hari Yanti, Made Sumadiyasa, and I. B. S. Manuaba. "The effect of various disturbances on the seeds on the content of chlorophyll a, chlorophyll b, carotenoids, and biomass of cayenne pepper Seedlings." BULETIN FISIKA 19, no. 1 (2018): 35. http://dx.doi.org/10.24843/bf.2018.v19.i01.p07.

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Study on the effect of various disturbances on the cayenne pepper seeds (Capsicum Frutescens L.) to the content of chlorophyll a, chlorophyll b, carotenoids, and biomass of the plants in the germination period have been done. The disturbances given include UV-C irradiation for 1 hour (S1), microgravity for 12 hours (S2), for 24 hours (S3), and for 48 hours (S4), as well as combined disturbances that are UV-C irradiation for 1 hour followed by microgravity for 12 hours (S5), for 24 hours (S6), and for 48 hours (S7). As comparison, it has been used seeds without treatment called control (S0). Th
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9

Shadrina, R. Yu, A. I. Yemets, and Ya B. Blume. "Autophagy development as an adaptive response to microgravity conditions in Arabidopsis thaliana." Faktori eksperimental'noi evolucii organizmiv 25 (August 30, 2019): 327–32. http://dx.doi.org/10.7124/feeo.v25.1186.

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Aim. The main aim of the study was to analyze the effect of microgravity on the growth and development of Arabidopsis thaliana seedlings at different time intervals of cultivation (4–10 days) and to investigate the development of autophagy induced by the conditions of microgravity in seedlings root cells. Methods. Microscopic methods as well as in vitro propagation method were used. To simulate of microgravity conditions plants were placed in clinostat machine. Results. In the course of experiments, the peaks of the formation of autophagosome were recorded: in the cells of the root cap zone of
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10

De Micco, Veronica, Michele Scala, and Giovanna Aronne. "Evaluation of the effect of clinostat rotation on pollen germination and tube development as a tool for selection of plants in Space." Acta Astronautica 58, no. 9 (2006): 464–70. http://dx.doi.org/10.1016/j.actaastro.2005.12.019.

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11

Gouws, Chrisna, Tanya Smit, Clarissa Willers, Hanna Svitina, Carlemi Calitz, and Krzysztof Wrzesinski. "Anticancer Potential of Sutherlandia frutescens and Xysmalobium undulatum in LS180 Colorectal Cancer Mini-Tumors." Molecules 26, no. 3 (2021): 605. http://dx.doi.org/10.3390/molecules26030605.

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Colorectal cancer remains to be one of the leading causes of death worldwide, with millions of patients diagnosed each year. Although chemotherapeutic drugs are routinely used to treat cancer, these treatments have severe side effects. As a result, the use of herbal medicines has gained increasing popularity as a treatment for cancer. In this study, two South African medicinal plants widely used to treat various diseases, Sutherlandia frutescens and Xysmalobium undulatum, were evaluated for potential activity against colorectal cancer. This potential activity for the treatment of colorectal ca
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12

Brown, Allan H., Anders Johnsson, David K. Chapman, and David Heathcote. "Gravitropic responses of the Avena coleoptile in space and on clinostats. IV. The clinostat as a substitute for space experiments." Physiologia Plantarum 98, no. 1 (1996): 210–14. http://dx.doi.org/10.1111/j.1399-3054.1996.tb00694.x.

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13

Shen-Miller, J., and R. R. Hinchman. "Nucleolar transformation in plants grown on clinostats." Protoplasma 185, no. 3-4 (1995): 194–204. http://dx.doi.org/10.1007/bf01272860.

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14

Steinitz, Benjamin, Th�r�se Best, and Kenneth L. Poff. "Phototropic fluence-response relations for Avena coleoptiles on a clinostat." Planta 176, no. 2 (1988): 189–95. http://dx.doi.org/10.1007/bf00392444.

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15

Sailer, H., P. Nick, and E. Sch�fer. "Inversion of gravitropism by symmetric blue light on the clinostat." Planta 180, no. 3 (1990): 378–82. http://dx.doi.org/10.1007/bf01160393.

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16

Galland, Paul, Heike Finger, and Yvonne Wallacher. "Gravitropism in Phycomyces: Threshold determination on a clinostat centrifuge." Journal of Plant Physiology 161, no. 6 (2004): 733–39. http://dx.doi.org/10.1078/0176-1617-01082.

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17

Nick, P., and E. Sch�fer. "Nastic response of maize (Zea mays L.) coleoptiles during clinostat rotation." Planta 179, no. 1 (1989): 123–31. http://dx.doi.org/10.1007/bf00395779.

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18

Dexheimer, Jean, Joëlle Gérard, and Patricia Genet. "Etude des modalités de la mycorhization de pivots d'Eucalyptus globulusdéveloppés en clinostat." Acta Botanica Gallica 141, no. 4 (1994): 511–16. http://dx.doi.org/10.1080/12538078.1994.10515191.

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19

Mischenko, L. T., T. Kiihne, I. A. Mischenko, and A. L. Boyko. "Infection process of wheat streak mosaic virus in clinostated Apogee wheat plants." Kosmìčna nauka ì tehnologìâ 8, no. 5-6 (2003): 211–15. http://dx.doi.org/10.15407/knit2003.05.211.

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20

MOORE, RANDY. "How Effectively Does a Clinostat Mimic the Ultrastructural Effects of Microgravity on Plant Cells?" Annals of Botany 65, no. 2 (1990): 213–16. http://dx.doi.org/10.1093/oxfordjournals.aob.a087926.

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21

Hoson, Takayuki, Seiichiro Kamisaka, Yoshio Masuda, and Masamichi Yamashita. "Changes in plant growth processes under microgravity conditions simulated by a three-dimensional clinostat." Botanical Magazine Tokyo 105, no. 1 (1992): 53–70. http://dx.doi.org/10.1007/bf02489403.

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22

Wang, Hui, Hui Qiong Zheng, Wei Sha, Rong Zeng, and Qi Chang Xia. "A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation." Journal of Experimental Botany 57, no. 4 (2006): 827–35. http://dx.doi.org/10.1093/jxb/erj066.

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23

Hoson, Takayuki, Seiichiro Kamisaka, Ryoichi Yamamoto, Masamichi Yamashita, and Yoshio Masuda. "Automorphosis of maize shoots under simulated microgravity on a three-dimensional clinostat." Physiologia Plantarum 93, no. 2 (1995): 346–51. http://dx.doi.org/10.1111/j.1399-3054.1995.tb02238.x.

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24

Kern, Volker D., Jochen M. Schwuchow, David W. Reed, et al. "Gravitropic moss cells default to spiral growth on the clinostat and in microgravity during spaceflight." Planta 221, no. 1 (2005): 149–57. http://dx.doi.org/10.1007/s00425-004-1467-3.

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25

Driss-Ecole, D., A. Cottignies, B. Jeune, F. Corbineau, and G. Perbal. "Increased mass production of Veronica arvensis grown on a slowly rotating clinostat." Environmental and Experimental Botany 34, no. 3 (1994): 303–10. http://dx.doi.org/10.1016/0098-8472(94)90051-5.

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26

Rivière, Mathieu, Yoann Corre, Alexis Peaucelle, Julien Derr, and Stéphane Douady. "The hook shape of growing leaves results from an active regulatory process." Journal of Experimental Botany 71, no. 20 (2020): 6408–17. http://dx.doi.org/10.1093/jxb/eraa378.

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Abstract The rachis of most growing compound leaves observed in nature exhibits a stereotypical hook shape. In this study, we focus on the canonical case of Averrhoa carambola. Combining kinematics and mechanical investigation, we characterize this hook shape and shed light on its establishment and maintenance. We show quantitatively that the hook shape is a conserved bent zone propagating at constant velocity and constant distance from the apex throughout development. A simple mechanical test reveals non-zero intrinsic curvature profiles for the rachis during its growth, indicating that the h
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27

Aronne, Giovanna, Veronica De Micco, Paolo Ariaudo, and Stefania De Pascale. "The effect of uni-axial clinostat rotation on germination and root anatomy ofPhaseolus vulgarisL." Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 137, no. 2 (2003): 155–62. http://dx.doi.org/10.1080/11263500312331351421.

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28

Legue, Valerie, Dominique Driss-Ecole, and Gerald Perbal. "Cell cycle and cell differentiation in lentil roots grown on a slowly rotating clinostat." Physiologia Plantarum 84, no. 3 (1992): 386–92. http://dx.doi.org/10.1111/j.1399-3054.1992.tb04680.x.

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29

Miyamoto, Kensuke, Ryoichi Yamamoto, Shuhei Fujii, et al. "Growth and Development in Arabidopsis thaliana through an Entire Life Cycle under Simulated Microgravity Conditions on a Clinostat." Journal of Plant Research 112, no. 4 (1999): 413–18. http://dx.doi.org/10.1007/pl00013865.

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30

Heathcote, D. G., and B. W. Bircher. "Enhancement of phototropic response to a range of light doses in Triticum aestivum coleoptiles in clinostat-simulated microgravity." Planta 170, no. 2 (1987): 249–56. http://dx.doi.org/10.1007/bf00397895.

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31

Link, Bruce M., and Daniel J. Cosgrove. "Analysis of Peg Formation in Cucumber Seedlings Grown on Clinostats and in a Microgravity (Space) Environment." Journal of Plant Research 112, no. 4 (1999): 507–16. http://dx.doi.org/10.1007/pl00013907.

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32

KNIGHT, C. D., and D. J. COVE. "The polarity of gravitropism in the moss Physcomitrella patens is reversed during mitosis and after growth on a clinostat." Plant, Cell and Environment 14, no. 9 (1991): 995–1001. http://dx.doi.org/10.1111/j.1365-3040.1991.tb00970.x.

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33

Saiki, Mizue, Hiroshi Fujita, Kouichi Soga, et al. "Cellular basis for the automorphic curvature of rice coleoptiles on a three-dimensional clinostat: possible involvement of reorientation of cortical microtubules." Journal of Plant Research 118, no. 3 (2005): 199–205. http://dx.doi.org/10.1007/s10265-005-0210-x.

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34

Lorenzi, Giovanna, and Gerald Perbal. "Root growth and statocyte polarity in lentil seedling roots grown in microgravity or on a slowly rotating clinostat." Physiologia Plantarum 78, no. 4 (1990): 532–37. http://dx.doi.org/10.1111/j.1399-3054.1990.tb05238.x.

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35

Brown, Allan H., David K. Chapman, Anders Johnsson, and David Heathcote. "Gravitropic responses of the Avena coleoptile in space and on clinostats. I. Gravitropic response thresholds." Physiologia Plantarum 95, no. 1 (1995): 27–33. http://dx.doi.org/10.1111/j.1399-3054.1995.tb00803.x.

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36

Johnsson, Anders, Allan H. Brown, David K. Chapman, David Heathcote, and Christina Karlsson. "Gravitropic responses of the Avena coleoptile in space and on clinostats. II. Is reciprocity valid?" Physiologia Plantarum 95, no. 1 (1995): 34–38. http://dx.doi.org/10.1111/j.1399-3054.1995.tb00804.x.

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37

Aarrouf, Jawad, Chantal Demandre, Nicole Darbelley, Claude Villard, and Gérald Perbal. "Development of the primary root and mobilisation of reserves in etiolated seedlings ofBrassica napus grown on a slowly rotating clinostat." Journal of Plant Physiology 160, no. 4 (2003): 409–13. http://dx.doi.org/10.1078/0176-1617-00857.

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38

Miyamoto, Kensuke, Tomoki Hoshino, Masamichi Yamashita, and Junichi Ueda. "Automorphosis of etiolated pea seedlings in space is simulated by a three-dimensional clinostat and the application of inhibitors of auxin polar transport." Physiologia Plantarum 123, no. 4 (2005): 467–74. http://dx.doi.org/10.1111/j.1399-3054.2005.00472.x.

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39

Villacampa, Alicia, Ludovico Sora, Raúl Herranz, Francisco-Javier Medina, and Malgorzata Ciska. "Analysis of Graviresponse and Biological Effects of Vertical and Horizontal Clinorotation in Arabidopsis thaliana Root Tip." Plants 10, no. 4 (2021): 734. http://dx.doi.org/10.3390/plants10040734.

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Clinorotation was the first method designed to simulate microgravity on ground and it remains the most common and accessible simulation procedure. However, different experimental settings, namely angular velocity, sample orientation, and distance to the rotation center produce different responses in seedlings. Here, we compare A. thaliana root responses to the two most commonly used velocities, as examples of slow and fast clinorotation, and to vertical and horizontal clinorotation. We investigate their impact on the three stages of gravitropism: statolith sedimentation, asymmetrical auxin dis
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40

Aarrouf, J., D. Schoëvaërt, R. Maldiney, and G. Perbal. "Changes in hormonal balance and meristematic activity in primary root tips on the slowly rotating clinostat and their effect on the development of the rapeseed root system." Physiologia Plantarum 105, no. 4 (1999): 708–18. http://dx.doi.org/10.1034/j.1399-3054.1999.105416.x.

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41

Hoshino, Tomoki, Kensuke Miyamoto, and Junichi Ueda. "Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum." Journal of Plant Research 120, no. 5 (2007): 619–28. http://dx.doi.org/10.1007/s10265-007-0103-2.

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42

Losinska-Sičiūnienė, Regina, Ramunė Stanevičienė, and Danguolė Švegždienė. "Effects of UVA and its simultaneous action with blue light on the growth and phototropism of cress leaves under various gravity conditions." Biologija 66, no. 2 (2020). http://dx.doi.org/10.6001/biologija.v66i2.4257.

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The gravity of the Earth (1g) and phototropic active components of the light spectrum are the stimuli regulating the directional growth of plants. In this study, the role of combined light and gravity effects on plant leaves (Lepidium sativum L.) was tested. Treatment with UVA (370 nm) or UVA combined with blue light together with the gravity in the slow clinostat (clinorotated 3 rpm), or Earth’s gravity (1g), was applied. A custom-built clinostat with LEDs allowed unidirectional illumination of cress leaves. UVA or UVA with blue light was directed laterally at cress leaves for a 3 h exposure.
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43

Xu, Huiyun, Jiawei Wu, Yuanyuan Weng, Jian Zhang, and Peng Shang. "Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells." Biologia 67, no. 1 (2012). http://dx.doi.org/10.2478/s11756-011-0161-8.

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AbstractThe aim of this study was to investigate the effects of the clinostat-simulated weightlessness on biological characteristics of MLO-Y4 osteocyte-like cells. MLO-Y4 cells were incubated for 24 h, then randomly divided into 3 groups and rotated in a clinostat as a model of simulated weightlessness for 12 h, 24 h and 48 h. The morphology, cytoskeleton, and secretion of soluble molecules of MLO-Y4 cells were observed and detected. The results show that clinostat culture affects the number of dendrites/cell, cytoskeleton distribution, and secretion of nitric oxide and prostaglandin E2 in ML
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44

Sailer, H., P. Nick, and E. Sch�fer. "Inversion of gravitropism by symmetric blue light on the clinostat." Planta 180, no. 3 (1990). http://dx.doi.org/10.1007/bf00198789.

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45

Miyamoto, Kensuke, Takahiro Yamasaki, Eiji Uheda, and Junichi Ueda. "Analysis of apical hook formation in Alaska pea with a 3-D clinostat and agravitropic mutant ageotropum." Frontiers in Plant Science 5 (April 8, 2014). http://dx.doi.org/10.3389/fpls.2014.00137.

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46

Medina, Brenda, and Cesar Rodríguez. "Análisis gravitrópico de la raíz primordial de plantas mesoamericanas, en condiciones de microgravedad simulada." Portal de la Ciencia, December 22, 2017, 73–96. http://dx.doi.org/10.5377/pc.v12i0.5518.

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Esta investigación muestra datos del crecimiento y curvatura gravitrópica de seis especies de importancia actual para el área Mesoamericana, Paseolus vulgaris var. Criolla, Paseolus vulgaris var. Amadeus 77, Phaseolus lunatus Phaseolus acutifolious, Vigna unguiculata y Sorghum bicolor; bajo condiciones de microgravedad simulada en clinostato de un eje, así como cortes histológicos de los tejidos de las muestras clinorotadas y las muestras Control 1g.Tiene como objetivo comprender el comportamiento de la raíz primordial de plantas actualmente cultivadas en Mesoamérica, cuando la fuerza neta sob
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