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1

Hammad, Muhammad, Onder Babur, Hamid Abdul Basit, and Mark Van Den Brand. "Clone-Seeker: Effective Code Clone Search Using Annotations." IEEE Access 10 (2022): 11696–713. http://dx.doi.org/10.1109/access.2022.3145686.

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2

EL-MATBOULI, M., and H. SOLIMAN. "Construction and screening of a cDNA library from the triactinomyxon spores ofMyxobolus cerebralis, the causative agent of salmonid Whirling Diseases." Parasitology 132, no. 4 (2006): 467–77. http://dx.doi.org/10.1017/s0031182005009522.

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The ZAP Express cDNA library was constructed using mRNA extracted from the triactinomyxon spores ofMyxobolus cerebralis. First-strand cDNA was synthesized using Moloney Murine leukaemia virus reverse transcriptase. Following second-strand cDNA synthesis, the double-stranded cDNA was digested withXhoI restriction enzyme, cDNA fragments less than 400 bp were removed and the remaining cDNA was ligated with the lambda ZAP Express vector. The recombinants were packagedin vitrousing Gigapack III gold packaging extract. The primary cDNA library titre contained 0·5×106clones, with 97% recombinant and
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3

Han, F. P., G. Fedak, T. Ouellet, H. Dan, and D. J. Somers. "Mapping of genes expressed in Fusarium graminearum-infected heads of wheat cultivar 'Frontana'." Genome 48, no. 1 (2005): 88–96. http://dx.doi.org/10.1139/g04-098.

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The isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of Triticum aestivum 'Frontana' resistant to Fusarium head blight is reported. A cDNA library was built from heads of 'Frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the pathogen Fusarium graminearum during infection. A group of 1794 clones was screened by dot blot hybridization for differential gene expression following infection. Twenty of these clones showed a strong difference in intensity of hybridization between infected and mock-inoculated wheat
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4

Farhadi, Mohammad Reza, Benjamin C. M. Fung, Yin Bun Fung, Philippe Charland, Stere Preda, and Mourad Debbabi. "Scalable code clone search for malware analysis." Digital Investigation 15 (December 2015): 46–60. http://dx.doi.org/10.1016/j.diin.2015.06.001.

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Abu-Helu, Rasmi. "Cloning and Expression of SARS COV-2 Surface Protein and its Use in Detecting Corona Viral Infections." Al-Quds Journal for Academic Research 01, no. 3 (2023): 28–34. http://dx.doi.org/10.47874/2023:pp:28-34.

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The main aim of this study was to develop indirect enzyme linked immune sorbent assay based on the use of bacterial cloned SARS CoV-2 spike protein as a cheap and continuously available source of antigen. This test was proved to be useful for a quantitative measurement and evaluation of antibody immune response among SARS-CoV-2 infected individuals. Standard cloning procedures had been used in cloning two different segments of the spike gene and its expression. The size of the two cloned segments were of 700 base pair and of 500 base pair, which were named clone 8 and clone 105, respectively.
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ZHANG, YU, XUMING CHEN, LIHUA WU, ZIQIANG LUO, and XIAOJIE LIU. "MHC-INSPIRED ANTIBODY CLONE ALGORITHM." International Journal of Computational Methods 07, no. 02 (2010): 299–318. http://dx.doi.org/10.1142/s0219876210002179.

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For solving complex optimization problems in some engineering applications, intelligent optimization algorithms based on biological mechanisms have better performance than traditional optimization algorithms. Most of these intelligent algorithms, however, have disadvantages in population diversity and preservation of elitist antibody genes, which lead to the degenerative phenomenon, the zigzag phenomenon, poor global optimization, and low convergence speed. Drawing inspiration from the features of major histocompatibility complex (MHC) in the biological immune system, we propose a novel MHC-in
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7

Zhang, Yu, Li Hua Wu, and Zi Qiang Luo. "Catastrophe-Based Antibody Clone Algorithm." Applied Mechanics and Materials 121-126 (October 2011): 4415–20. http://dx.doi.org/10.4028/www.scientific.net/amm.121-126.4415.

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In solving complex optimization problems, intelligent optimization algorithms such as immune algorithm show better advantages than traditional optimization algorithms. Most of these immune algorithms, however, have disadvantages in population diversity and preservation of elitist antibodies genes, which will lead to the degenerative phenomenon, the zigzag phenomenon, poor global optimization, and low convergence speed. By introducing the catastrophe factor into the ACAMHC algorithm, we propose a novel catastrophe-based antibody clone algorithm (CACA) to solve the above problems. CACA preserves
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8

S K, Radhika. "Spotify Clone – Melodix : A Review." INTERANTIONAL JOURNAL OF SCIENTIFIC RESEARCH IN ENGINEERING AND MANAGEMENT 09, no. 01 (2025): 1–9. https://doi.org/10.55041/ijsrem41242.

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This paper explores the development of a Spotify clone app, focusing on its core features and functionality to provide a seamless music streaming experience. By integrating user-friendly interfaces, personalized playlists, and advanced search capabilities, the app aims to replicate Spotify's success while offering customizable options. The study examines the technical architecture, including the backend, database management, and music recommendation algorithms. It also highlights the importance of scalability, security, and user engagement, suggesting that such an app has the potential to revo
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9

Keivanloo, Iman, Chanchal K. Roy, and Juergen Rilling. "SeByte: Scalable clone and similarity search for bytecode." Science of Computer Programming 95 (December 2014): 426–44. http://dx.doi.org/10.1016/j.scico.2013.10.006.

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10

Kalinin, Maxim, and Nikita Gribkov. "Syntactic–Semantic Detection of Clone-Caused Vulnerabilities in the IoT Devices." Sensors 24, no. 22 (2024): 7251. http://dx.doi.org/10.3390/s24227251.

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This paper addresses the problem of IoT security caused by code cloning when developing a massive variety of different smart devices. A clone detection method is proposed to identify clone-caused vulnerabilities in IoT software. A hybrid solution combines syntactic and semantic analyses of the code. Based on the recovered code, an attributed abstract syntax tree is constructed for each code fragment. All nodes of the commonly used abstract syntax tree are proposed to be weighted with semantic attribute vectors. Each attributed tree is then encoded as a semantic vector using a Deep Graph Neural
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11

Shyam, Kaushik, Kaur Ramandeep, Rani Mansha, Kashyap Anurag, and Khan Anas. "Frontend Amazon Clone using HTML CSS and Java Script." Frontend Amazon Clone using HTML CSS and Java Script 8, no. 11 (2023): 6. https://doi.org/10.5281/zenodo.10394475.

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The Amazon Frontend Clone Project is a web development that attempts to replicate the user interface and design of Amazon's popular e-commerce platform using a combination of HTML, CSS and JavaScript. Our ultimate goal is to create an attractive, functional, and visually faithful clone of Amazon's frontend that allows users to seamlessly browse products, view product details and simulate the shopping experience. We will use HTML to build the website, CSS including responsive design for multiple devices, and JavaScript to implement dynamic features such as product filtering, search functionalit
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Pereira, Bárbara Luísa Corradi, Aylson Costa Oliveira, Ana Márcia Macedo Ladeira Carvalho, Angélica de Cássia Oliveira Carneiro, Larissa Carvalho Santos, and Benedito Rocha Vital. "Quality of Wood and Charcoal fromEucalyptusClones for Ironmaster Use." International Journal of Forestry Research 2012 (2012): 1–8. http://dx.doi.org/10.1155/2012/523025.

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Considering the wide variety of species and clones ofEucalyptuscultivated in Brazil, it is necessary to search for new information on wood properties, so that the selection of genetically superior material may be successful. The present study aimed to determine the properties of wood and charcoal from different clones ofEucalyptusspp. Six clones at the age of 7.5 years were evaluated and the samples were from a clonal, located in the city of Lassance, Minas Gerais, Brazil. Basic density, chemical composition, and higher heating value were determined. Carbonizations in a laboratory kiln were do
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13

Wei, Y. F., P. Robins, K. Carter, et al. "Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination." Molecular and Cellular Biology 15, no. 6 (1995): 3206–16. http://dx.doi.org/10.1128/mcb.15.6.3206.

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Three distinct DNA ligases, I to III, have been found previously in mammalian cells, but a cloned cDNA has been identified only for DNA ligase I, an essential enzyme active in DNA replication. A short peptide sequence conserved close to the C terminus of all known eukaryotic DNA ligases was used to search for additional homologous sequences in human cDNA libraries. Two different incomplete cDNA clones that showed partial homology to the conserved peptide were identified. Full-length cDNAs were obtained and expressed by in vitro transcription and translation. The 103-kDa product of one cDNA clo
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14

Tan, Yan Song. "An Improved Immune Clone Algorithm Logistics Delivery Strategy." Journal of Advanced Computational Intelligence and Intelligent Informatics 23, no. 2 (2019): 309–12. http://dx.doi.org/10.20965/jaciii.2019.p0309.

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Logistics routing problem is a typical NP hard problem, which is very difficult to solve accurately. On the basis of establishing logistics path optimization model, an immune clone algorithm is proposed. To improve the accuracy of search algorithms, the clonal selection and high frequency variations in the immune algorithm method are introduced. Then the antibody encoding virtual distribution point algorithm is designed to improve search efficiency. The benchmark problem of logistics delivery path optimization is simulated and analyzed. Experimental results show that the proposed immune clonin
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15

Gérard, C. M., C. Mollereau, G. Vassart, and M. Parmentier. "Molecular cloning of a human cannabinoid receptor which is also expressed in testis." Biochemical Journal 279, no. 1 (1991): 129–34. http://dx.doi.org/10.1042/bj2790129.

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A cDNA clone encoding a receptor protein which presents all the characteristics of a guanine-nucleotide-binding protein (G-protein)-coupled receptor was isolated from a human brain stem cDNA library. The probe used (HGMP08) was a 600 bp DNA fragment amplified by a low-stringency PCR, using human genomic DNA as template and degenerate oligonucleotide primers corresponding to conserved sequences amongst the known G-protein-coupled receptors. The deduced amino acid sequence encodes a protein of 472 residues which shares 97.3% identity with the rat cannabinoid receptor cloned recently [Matsuda, Lo
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16

Sundara Rao, Dr T. Syam, P. Venkata Snehalatha, R. Lakshmi Naga Chaitanya, M. Aparna, and Sk Haleema Kusum. "RAG -CLONE A Generic Framework." International Scientific Journal of Engineering and Management 04, no. 03 (2025): 1–6. https://doi.org/10.55041/isjem02315.

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This paper presents a RAG system (Retrieval Augmented Generation)that aims to improve how AI processes, accesses ,and generates information. Our approach uses Vector embedding to improve data absorption and provide more accurate and contextual answers using FAISS-based on the similarity and the mistral searches. The system is created to process unstructured, unstructured data from a variety of sources, including PDFs and Excel files. Users can interact with text-based queries as well as voice commands. To make this simple, we integrate Whisper AI for speech recognition, allowing users to ask q
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17

Lopez Rubio, Montserrat, Anna Gaya, Marta Morado, Vicente Carrasco, Ataulfo Gonzalez Fernandez, and Ana Villegas. "Relationship Between Myelodysplastic Syndrome and Paroxysmal Nocturnal Hemoglobinuria: Spanish Erythropathology Group and Spanish Paroxysmal Nocturnal Hemoglobinuria Working Group Experience." Blood 126, no. 23 (2015): 4546. http://dx.doi.org/10.1182/blood.v126.23.4546.4546.

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Abstract INTRODUCTION PNH clones appear frequently in patients with myelodysplastic syndrome (MDS) and aplastic anemia (AA) which may even evolve into classic PNH. It has been described isolated cases of clonal evolution from AA, through classic PNH and MDS; and at the end, acute leukemia (AL) has rarely been described. These patients show a progressive replacement of the PNH clone by a dysplastic one carrying a monosomy of chromosome 7. OBJECTIVE We designed a retrospective study of patients included in the database of the Spanish PNH Registry in search of patients with a MDS and a PNH clone
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18

CHEN, JIANYONG, QIUZHEN LIN, and LINLIN SHEN. "AN IMMUNE-INSPIRED EVOLUTION STRATEGY FOR CONSTRAINED OPTIMIZATION PROBLEMS." International Journal on Artificial Intelligence Tools 20, no. 03 (2011): 549–61. http://dx.doi.org/10.1142/s0218213011000279.

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Based on clonal selection principle, this paper proposes an immune-inspired evolution strategy (IIES) for constrained optimization problems with two improvements. Firstly, in order to enhance global search capability, more clones are produced by individuals that have far-off nearest neighbors in the less-crowed regions. On the other hand, immune update mechanism is proposed to replace the worst individuals in clone population with the best individuals stored in immune memory in every generation. Therefore, search direction can always focus on the fittest individuals. These proposals are able t
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19

Park, Jin-woo, Mu-Woong Lee, Jong-Won Roh, Seung-won Hwang, and Sunghun Kim. "Surfacing code in the dark: an instant clone search approach." Knowledge and Information Systems 41, no. 3 (2013): 727–59. http://dx.doi.org/10.1007/s10115-013-0677-z.

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20

Katsu, Yoshinao, Shin Oana, Xiaozhi Lin, Susumu Hyodo, Laurent Bianchetti, and Michael E. Baker. "Cloning of nine glucocorticoid receptor isoforms from the slender African lungfish (Protopterus dolloi)." PLOS ONE 17, no. 8 (2022): e0272219. http://dx.doi.org/10.1371/journal.pone.0272219.

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We wanted to clone the glucocorticoid receptor (GR) from slender African lungfish (Protopterus dolloi) for comparison to the P. dolloi mineralocorticoid receptor (MR), which we had cloned and were characterizing, as well as for comparison to the GRs from humans, elephant shark and zebrafish. However, although sequencing of the genome of the Australian lungfish (Neoceratodus forsteri), as well as, that of the West African lungfish (Protopterus annectens) were reported in the first three months of 2021, we could not retrieve a GR sequence with a BLAST search of GenBank, when we submitted our res
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21

Giorda, R., and H. L. Ennis. "Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes." Molecular and Cellular Biology 7, no. 6 (1987): 2097–103. http://dx.doi.org/10.1128/mcb.7.6.2097-2103.1987.

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A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-aci
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22

Giorda, R., and H. L. Ennis. "Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes." Molecular and Cellular Biology 7, no. 6 (1987): 2097–103. http://dx.doi.org/10.1128/mcb.7.6.2097.

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A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-aci
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23

Fritzler, M. J., J. C. Hamel, R. L. Ochs, and E. K. Chan. "Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex." Journal of Experimental Medicine 178, no. 1 (1993): 49–62. http://dx.doi.org/10.1084/jem.178.1.49.

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Serum autoantibodies from a patient with autoantibodies directed against the Golgi complex were used to screen clones from a HepG2 lambda Zap cDNA library. Three related clones, designated SY2, SY10, and SY11, encoding two distinct polypeptides were purified for further analysis. Antibodies affinity purified by adsorption to the lambda Zap-cloned recombinant proteins and antibodies from NZW rabbits immunized with purified recombinant proteins reproduced Golgi staining and bound two different proteins, 95 and 160 kD, from whole cell extracts. The SY11 protein was provisionally named golgin-95 a
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24

Castañeda-Sánchez, Jorge I., Everardo Curiel-Quesada, Miguel Pedrosa-Seres, Mario E. Cancino-Diaz, Sandra Rodríguez-Martínez, and Juan C. Cancino-Diaz. "Peptidic sequence “HSEAETGPP” is recognized by the sera of pars planitis patients." Clinical & Investigative Medicine 32, no. 3 (2009): 206. http://dx.doi.org/10.25011/cim.v32i3.6109.

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Purpose: HLA class II, p-36 protein, heat shock protein and retinal antigens have been associated with pars planitis (PP), but their participation in the development of the disease are unknown. A search for new molecules related to PP is necessary. This work focused on the identification of peptides recognized by PP patient sera using the phage display method. Methods: Sera of PP patients were used to isolate peptides fused to M13-phage pIII protein. The response of PP and healthy sera to peptides was determined by ELISA. PCR amplification and sequencing of peptide-encoding fragments from clon
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25

Rokka, V. M., M. S. Clark, D. L. Knudson, E. Pehu, and NLV Lapitan. "Cytological and molecular characterization of repetitive DNA sequences of Solanum brevidens and Solanum tuberosum." Genome 41, no. 4 (1998): 487–94. http://dx.doi.org/10.1139/g98-047.

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The chromosomal distribution, copy numbers, and nucleotide sequences were determined for four repetitive DNA clones, pSB1 and pSB7 of Solanum brevidens and pST3 and pST10 of Solanum tuberosum. Using fluorescence in situ hybridization (FISH), pSB1 and pSB7 were localized near the telomeres and in some centromeric and interstitial sites of S. brevidens chromosomes, but not in S. tuberosum chromosomes, after high stringency washes. The clone pST3 showed signals in the telomeric areas of a few chromosomes in S. tuberosum, but signals were not detected in S. brevidens. All three repeated sequences
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Delandre, Océane, Ombeline Lamer, Jean-Marie Loreau, et al. "Long-Read Sequencing and De Novo Genome Assembly Pipeline of Two Plasmodium falciparum Clones (Pf3D7, PfW2) Using Only the PromethION Sequencer from Oxford Nanopore Technologies without Whole-Genome Amplification." Biology 13, no. 2 (2024): 89. http://dx.doi.org/10.3390/biology13020089.

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Antimalarial drug resistance has become a real public health problem despite WHO measures. New sequencing technologies make it possible to investigate genomic variations associated with resistant phenotypes at the genome-wide scale. Based on the use of hemisynthetic nanopores, the PromethION technology from Oxford Nanopore Technologies can produce long-read sequences, in contrast to previous short-read technologies used as the gold standard to sequence Plasmodium. Two clones of P. falciparum (Pf3D7 and PfW2) were sequenced in long-read using the PromethION sequencer from Oxford Nanopore Techno
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27

Nishi, Manziba Akanda, and Kostadin Damevski. "Scalable code clone detection and search based on adaptive prefix filtering." Journal of Systems and Software 137 (March 2018): 130–42. http://dx.doi.org/10.1016/j.jss.2017.11.039.

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28

Ragkhitwetsagul, Chaiyong, and Jens Krinke. "Siamese: scalable and incremental code clone search via multiple code representations." Empirical Software Engineering 24, no. 4 (2019): 2236–84. http://dx.doi.org/10.1007/s10664-019-09697-7.

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29

Serce, S., and J. F. Hancock. "195 Sexual and Vegetative Performance of Native and Cultivated Day-neutral Strawberries under Moderate and High Temperatures." HortScience 35, no. 3 (2000): 424D—424. http://dx.doi.org/10.21273/hortsci.35.3.424d.

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A common complaint with day-neutral strawberries is that they perform poorly in mid-summer heat. Since most modern day-neutral cultivars are derived from the same Fragaria virginiana ssp. glauca clone from Utah, we felt it prudent to search for alternate sources of day-neutrality that were more heat-tolerant. We compared the sexual and vegetative performance of nine F. virginiana clones from a wide range of environments including the Utah site, and four F. × ananassa day-neutral types (`Aromas', `Fort Laramie', `Ogallala', and `Tribute') under constant temperatures of 18, 22, 26, and 30 °C and
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Pawlik, Piotr, Kristiana K. Grigoriadis, Abigail Bunkum, Simone Zaccaria, Nicholas McGranahan, and Charles Swanton. "Abstract 1200: A novel algorithm for deconvolving cancer allele-specific clone copy number and copy number evolution." Cancer Research 84, no. 6_Supplement (2024): 1200. http://dx.doi.org/10.1158/1538-7445.am2024-1200.

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Abstract Both single nucleotide variants (SNVs) and somatic copy number alterations (SCNAs) accumulate in cancer cells during tumor development, fuelling clonal evolution. However, accurate inference of their coevolution from bulk DNA sequencing is challenging. We present ALPACA (ALlele-specific Phylogenetic Analysis of clone Copy number Alterations), a novel algorithm to allow the practical inference of SNV and SCNA coevolution. ALPACA is framed as an optimisation problem, that takes as input bulk tumor sample copy number mixtures and the tumor phylogenetic tree constructed from SNVs, and dec
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Zhou, Xu, Yanheng Liu, and Bin Li. "A multi-objective discrete cuckoo search algorithm with local search for community detection in complex networks." Modern Physics Letters B 30, no. 07 (2016): 1650080. http://dx.doi.org/10.1142/s0217984916500809.

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Detecting community is a challenging task in analyzing networks. Solving community detection problem by evolutionary algorithm is a heated topic in recent years. In this paper, a multi-objective discrete cuckoo search algorithm with local search (MDCL) for community detection is proposed. To the best of our knowledge, it is first time to apply cuckoo search algorithm for community detection. Two objective functions termed as negative ratio association and ratio cut are to be minimized. These two functions can break through the modularity limitation. In the proposed algorithm, the nest location
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ISHIO, Takashi, Naoto MAEDA, Kensuke SHIBUYA, Kenho IWAMOTO, and Katsuro INOUE. "NCDSearch: Sliding Window-Based Code Clone Search Using Lempel-Ziv Jaccard Distance." IEICE Transactions on Information and Systems E105.D, no. 5 (2022): 973–81. http://dx.doi.org/10.1587/transinf.2021edp7222.

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Bonnicksen, Andrea L. "Creating a Clone in Ninety Days: In Search of a Cloning Policy." Politics and the Life Sciences 16, no. 2 (1997): 304–8. http://dx.doi.org/10.1017/s0730938400024874.

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34

Lionberger, Jack M., Ninder K. Panesar, Bharat Panuganti, Ahmad Rajeh, and Carl E. Freter. "Detecting Accumulated Somatic Mutations in Healthy Aged Female Myeloid Cells." Blood 128, no. 22 (2016): 5045. http://dx.doi.org/10.1182/blood.v128.22.5045.5045.

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Abstract Background: Acute Myeloid Leukemia and Myelodysplasia are diseases associated with age and with genetic mutations driving evolution of clonal dominance. The earliest steps leading to genetic variation occur in a milieu of normal hematopoiesis by as a matter of course because genomic divergence is a fundamental element of biology. Organism speciation implicates genetic variation is the first step of evolution, followed by resource restriction pressure (a bottleneck). Such conditions create an environment where competitive advantages arise for genetically unique sub-species. Our work is
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Doosti, Abbas, Ghasemi Dehkordi, and Hosein Khodabakhsh. "cDNA cloning and sequencing of ostrich Growth hormone." Archives of Biological Sciences 64, no. 2 (2012): 445–49. http://dx.doi.org/10.2298/abs1202445d.

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In recent years, industrial breeding of ostrich (Struthio camelus) has been widely developed in Iran. Growth hormone (GH) is a peptide hormone that stimulates growth and cell reproduction in different animals. The aim of this study was to clone and sequence the ostrich growth hormone gene in E. coli, done for the first time in Iran. The cDNA that encodes ostrich growth hormone was isolated from total mRNA of the pituitary gland and amplified by RT-PCR using GH specific PCR primers. Then GH cDNA was cloned by T/A cloning technique and the construct was transformed into E. coli. Finally, GH cDNA
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Griscelli-Bennaceur, A., E. Gluckman, ML Scrobohaci, et al. "Aplastic anemia and paroxysmal nocturnal hemoglobinuria: search for a pathogenetic link." Blood 85, no. 5 (1995): 1354–63. http://dx.doi.org/10.1182/blood.v85.5.1354.bloodjournal8551354.

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The association of paroxysmal nocturnal hemoglobinuria (PNH) and aplastic anemia (AA) raises the yet unresolved questions as to whether these two disorders are different forms of the same disease. We compared two groups of patients with respect to cytogenetic features, glycosylphosphatidylinositol (GPI)-linked protein expression, protein C/protein S/thrombomodulin/antithrombin III activity, and PIG-A gene expression. The first group consisted of eight patients with PNH (defined as positive Ham and sucrose tests at diagnosis), and the second, 37 patients with AA. Twelve patients with AA later d
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Pereira, Leonardo T., and Claudio F. M. Toledo. "Speeding up Search-Based Algorithms for Level Generation in Physics-Based Puzzle Games." International Journal on Artificial Intelligence Tools 26, no. 05 (2017): 1760019. http://dx.doi.org/10.1142/s0218213017600193.

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This research uses Machine Learning (ML) techniques in order to aid search-based (SB) algorithms to improve level generation for physics-based puzzle games. These algorithms’ performance are improved by reducing simulation time when the ML techniques are applied. Classification algorithms prevent levels with undesired traits to be evaluated during the simulation phase of the SB procedures. An Angry Birds clone is used for conducting the experiments and results report improvement using the combined approach against every SB algorithm by itself.
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Wilson, G. L., C. H. Fox, A. S. Fauci, and J. H. Kehrl. "cDNA cloning of the B cell membrane protein CD22: a mediator of B-B cell interactions." Journal of Experimental Medicine 173, no. 1 (1991): 137–46. http://dx.doi.org/10.1084/jem.173.1.137.

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We have cloned a full-length cDNA for the B cell membrane protein CD22, which is referred to as B lymphocyte cell adhesion molecule (BL-CAM). Using subtractive hybridization techniques, several B lymphocyte-specific cDNAs were isolated. Northern blot analysis with one of the clones, clone 66, revealed expression in normal activated B cells and a variety of B cell lines, but not in normal activated T cells, T cell lines, Hela cells, or several tissues, including brain and placenta. One major transcript of approximately 3.3 kb was found in B cells although several smaller transcripts were also p
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Sobrinho-Simões, Manuel, Vicki Wilczek, Joannah Score, Nicholas C. P. Cross, Jane F. Apperley, and Junia V. Melo. "In search of the original leukemic clone in chronic myeloid leukemia patients in complete molecular remission after stem cell transplantation or imatinib." Blood 116, no. 8 (2010): 1329–35. http://dx.doi.org/10.1182/blood-2009-11-255109.

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Abstract It is not clear if absence of BCR-ABL transcripts—complete molecular response (CMR)—is synonymous with, or required for, cure of chronic myeloid leukemia (CML). Some patients achieve CMR with imatinib (IM), but most relapse shortly after treatment discontinuation. Furthermore, most patients in long-term remission (LTR) post–stem cell transplantation (SCT) are considered functionally cured, although some remain occasionally positive for low-level BCR-ABL mRNA. Interpretation of the latter is complicated because it has been observed in healthy subjects. We designed a patient-specific, h
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Sriramulu, Dinesh Diraviam. "Small Heat Shock Proteins Produced by Pseudomonas Aeruginosa Clonal Variants Isolated from Diverse Niches." Proteomics Insights 2 (January 2009): PRI.S3760. http://dx.doi.org/10.4137/pri.s3760.

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Genomic islands interspersed in the chromosome of P. aeruginosa led to inter- and intraclonal diversity. Recently, a particular clone of P. aeruginosa called clone C was isolated from cystic fibrosis (CF) patients, clinical and non-clinical habitats throughout Europe and in Canada. P. aeruginosa clone C strains harbour up to several hundred acquired genes involved in the adaptation of bacteria to diverse niches. Two genes ( hp25 and hp18) from one of the hypervariable regions in the chromosome of clone C strains were highly expressed under standard culture conditions as well as under condition
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41

Heath, Victoria, Elaine Cloutman-Green, Samuel Watkin, et al. "Staphylococcus capitis: Review of Its Role in Infections and Outbreaks." Antibiotics 12, no. 4 (2023): 669. http://dx.doi.org/10.3390/antibiotics12040669.

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In June 2021, a national incident team was formed due to an increased detection of Staphylococcus capitis in samples from hospitalised infants. Staphylococcus capitis has been known to cause outbreaks in neonatal units across the globe, but the extent of the UK spread was unclear. A literature review was undertaken to support case identification, clinical management and environmental infection control. A literature search was undertaken on multiple databases from inception to 24 May 2021, using keywords such as “Staphylococcus capitis”, “NRCS-A”, “S. capitis”, “neonate”, “newborn” and “neonata
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Beer, Philip A., François Delhommeau, Jean-Pierre LeCouédic, et al. "Two routes to leukemic transformation after a JAK2 mutation–positive myeloproliferative neoplasm." Blood 115, no. 14 (2010): 2891–900. http://dx.doi.org/10.1182/blood-2009-08-236596.

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Abstract Acute myeloid leukemia (AML) may follow a JAK2-positive myeloproliferative neoplasm (MPN), although the mechanisms of disease evolution, often involving loss of mutant JAK2, remain obscure. We studied 16 patients with JAK2-mutant (7 of 16) or JAK2 wild-type (9 of 16) AML after a JAK2-mutant MPN. Primary myelofibrosis or myelofibrotic transformation preceded all 7 JAK2-mutant but only 1 of 9 JAK2 wild-type AMLs (P = .001), implying that JAK2-mutant AML is preceded by mutation(s) that give rise to a “myelofibrosis” phenotype. Loss of the JAK2 mutation by mitotic recombination, gene conv
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Yin-Hua, Chen, Ouyang Bo, Li Han-Xia, and Ye Zhi-Biao. "Cloning of ACO gene and inhibition of ethylene evolution in tomatoes with RNAi." Chinese Journal of Agricultural Biotechnology 4, no. 3 (2007): 193–97. http://dx.doi.org/10.1017/s1479236207001775.

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AbstractA 1018 bp fragment of the ACO gene cDNA sequence was cloned from tomato (Lycopersicon esculentum) leaves incubated with a pathogen mixture using reverse transcriptase-polymerase chain reaction (RT-PCR) with two PCR primers designed according to the sequence of a tomato cDNA clone (E11). A BLAST search showed the sequence presenting a very high match with the ACO genes in other plants, with 83–99% homology. Using this sequence, an RNA interference (RNAi) transformation vector (pD311) was constructed and transformed into tomato. Twenty-seven regenerated plants with kanamycin resistance w
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44

G, Anbarasi, and Vishnupriya B. "Molecular identification and phylogenetic analysis of suaeda maritima from parangipettai coastal areas, southeast coast of india." Kongunadu Research Journal 7, no. 1 (2020): 28–34. http://dx.doi.org/10.26524/krj.2020.5.

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Conventional taxonomy is limited with delineating species and controversies arise with DNA barcoding based identifications. Hence, an alternative supporting approach is very much needed to identify species and differentiate them within the species based on the genetic material. 18S rRNA genes have been particularly helpful in analyzing phylogeny at the species level. In addition, bioinformatics which represents a new, growing area of science uses computational approaches to answer biological questions. Salt tolerant costal salt marsh plant of Suaeda maritima was selected for 18s rRNA sequencin
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Popov, Alexander M., Svetlana A. Kashpor, Tatiana Yu Verzhbitskaya, et al. "FLAER-negative granulocytes detection in periferal blood is simple and highly reproducible test for pnh screening." Pediatric Hematology/Oncology and Immunopathology 15, no. 3 (2016): 12–18. https://doi.org/10.24287/1726-1708-2016-15-3-12-18.

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GPI-deficient cells' detection by multicolor flow cytometry using fluorescently labeled aerolysin (FLAER) currently is a standard approach for PNH diagnostics. Nevertheless more simple and cheap approach with high sensitivity is needed for routine PNH screening. The aim of present study was to investigate the applicability of simplified two-color (FLAER/CD15) flow cytometry for PNH clone screening. Search for FLAER-negative granulocytes was performed in 259 peripheral blood samples obtained from the patients with suspected PNH. Symplified analysis results were compared to the multicolor data.
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Kulyk, Ya S. "EPITOPES IDENTIFICATION OF BROADLY NEUTRALIZING MONOCLONAL ANTIBODIES AGAINST Corynebacterium diphtheriae EXOTOXIN." Biotechnologia Acta 15, no. 4 (2022): 37–40. http://dx.doi.org/10.15407/biotech15.04.037.

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Background. Better and high-potency vaccines against diphtheria are urgently needed to provide broader protection against diverse strains and subtypes. Identification of novel broadly neutralizing epitopes targeted by protective antibodies could aid in such efforts. Aim. In this study we focused on the search of binding sites identification of anti diphtheria toxin monoclonal antibodies and their neutralizing activity to block binding of recombinant exotoxin derivates with host receptors. Methods. Vero cells were cultured in the complete RPMI-1640 medium under standard conditions and used for
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Trusty, Andrew, Santiago Santiago Ontañón, and Ashwin Ram. "Stochastic Plan Optimization in Real-Time Strategy Games." Proceedings of the AAAI Conference on Artificial Intelligence and Interactive Digital Entertainment 4, no. 1 (2021): 126–31. http://dx.doi.org/10.1609/aiide.v4i1.18684.

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We present a domain independent off-line adaptation technique called Stochastic Plan Optimization for finding and improving plans in real-time strategy games. Our method is based on ideas from genetic algorithms but we utilize a different representation for our plans and an alternate initialization procedure for our search process. The key to our technique is the use of expert plans to initialize our search in the most relevant parts of plan space. Our experiments validate this approach using our existing case based reasoning system Darmok in the real-time strategy game Wargus, a clone of Warc
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García García, José, Pascual Romero Azorín, Benjamín García García, Begoña García Castellanos, Pablo Botía, and Josefa María Navarro. "Sustainability of Vine Cultivation in Arid Areas of Southeastern Spain through Strategies Combining Controlled Deficit Irrigation and Selection of Monastrell Clones." Agronomy 13, no. 8 (2023): 2046. http://dx.doi.org/10.3390/agronomy13082046.

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The use of rootstocks and clones with different vigor and sensitivity to water deficit is an important and useful agronomic tool for vineyard management. It is essential to search for systems and strategies that maximize efficiency and productivity in the use of water. The objective of this work was to carry out a comparative economic and environmental evaluation, using the life cycle cost (LCC) and life cycle assessment (LCA) methodology, of the organic cultivation of seven Monastrell clones under two irrigation strategies, as well as to evaluate the productivity of water. The grape productio
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Sørensen, Annette Balle, Anders H. Lund, Steen Ethelberg, Neal G. Copeland, Nancy A. Jenkins, and Finn Skou Pedersen. "Sint1, a Common Integration Site in SL3-3-Induced T-Cell Lymphomas, Harbors a Putative Proto-Oncogene with Homology to the Septin Gene Family." Journal of Virology 74, no. 5 (2000): 2161–68. http://dx.doi.org/10.1128/jvi.74.5.2161-2168.2000.

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ABSTRACT The murine retrovirus SL3-3 is a potent inducer of T-cell lymphomas when inoculated into susceptible newborn mice. Previously, DNAs from twenty SL3-3-induced tumors were screened by PCR for provirus integration sites. Two out of 20 tumors demonstrated clonal provirus insertion into a common region. This region has now been isolated and characterized. The region, named SL3-3 integration site 1 (Sint1), maps to the distal end of mouse chromosome 11, corresponding to human chromosome 17q25, and may be identical to a mouse mammary tumor virus integration site in a T-cell lymphoma,Pad3. Tw
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Ishii, Kotaro, Ryuji Sugiyama, Megumi Onuki, Yusuke Kazama, Sachihiro Matsunaga, and Shigeyuki Kawano. "The Y chromosome-specific STS marker MS2 and its peripheral regions on the Y chromosome of the dioecious plant Silene latifolia." Genome 51, no. 4 (2008): 251–60. http://dx.doi.org/10.1139/g08-005.

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Sex determination in Silene latifolia uses the XX/XY system. The recent evolution of dioecy in S. latifolia provides a unique opportunity to study the early stages of Y chromosome evolution. However, the current Y chromosome map still contains many large gaps with no available markers. In this study, a sequence tagged site (STS) marker, MS2, was isolated and mapped to the same locus as L8 on the Y chromosome. To investigate the peripheral regions of MS2, a bacterial artificial chromosome (BAC) library was constructed from a male plant, and the BAC clone containing MS2 (MS2-9d12F) was isolated
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