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Relevant bibliographies by topics / Cloning, Molecular – methods

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Academic literature on the topic 'Cloning, Molecular – methods'

Author: Grafiati

Published: 4 June 2021

Last updated: 17 February 2022

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Journal articles on the topic "Cloning, Molecular – methods"

1

Tan, Lendl, Emily J. Strong, Kyra Woods, and Nicholas P. West. "Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method." PeerJ 6 (June 29, 2018): e5146. http://dx.doi.org/10.7717/peerj.5146.

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Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min. HAC facilitates insert integration due to a sequence alignment strategy, by way of short, vector-specific homology tails appended to insert during amplification. Simultaneous exposure of single-stranded fragment ends, utilising the 3′→5′ exonuclease activity of T4 DNA polymerase, creates overlapping homologous DNA on each molecule. The exonuclease activity of T4 polymerase is quenched

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Silverman, Sanford J. "Methods for molecular cloning and analysis of eukaryotic genes." Analytical Biochemistry 192, no. 1 (1991): 254. http://dx.doi.org/10.1016/0003-2697(91)90217-h.

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Alfaresi, Mubarak, and Bassam Mahboub. "Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods." Open Microbiology Journal 11, no. 1 (2017): 384–86. http://dx.doi.org/10.2174/1874285801711010384.

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Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing. Results: All isolat

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Karn, Jonathan. "Methods in enzymology, vol. 152: guide to molecular cloning techniques." Trends in Genetics 4, no. 9 (1988): 268. http://dx.doi.org/10.1016/0168-9525(88)90035-2.

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Cline, Steven W., Wan L. Lam, Robert L. Charlebois, Leonard C. Schalkwyk, and W. Ford Doolittle. "Transformation methods for halophilic archaebacteria." Canadian Journal of Microbiology 35, no. 1 (1989): 148–52. http://dx.doi.org/10.1139/m89-022.

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We present a practical description of polyethylene glycol mediated spheroplast transformation of Halobacterium halobium and Halobacterium volcanii. This method has been applied to phage DNA transfection, plasmid DNA transformation, and transformation with linear fragments of high molecular weight genomic DNA. Efficient spheroplast regeneration allows uncomplicated recovery of transformed progeny. Transformations can be performed equally well using fresh or frozen cell preparations. These methods should find application in molecular cloning, genetic fine mapping, and strain construction.Key wor

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Миненко, I. Minenko, Сердюков, and D. Serdyukov. "To The Question of the Cloninig History." Journal of New Medical Technologies. eJournal 8, no. 1 (2014): 1–9. http://dx.doi.org/10.12737/2907.

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Cloning in biology - cloning in biology is getting both asexually by natural means, including vegetative reproduction, and by means of biotechnological methods genetically identical organisms, cells or molecules. In the XXI century, in the conditions of the impetuous growth of the population and the food problem aggravation, humanity faces a growing and more demanding challenge of creating the necessary quantity of living organisms-plants and animals, with pre-defined properties: resistant to disease, climate fluctuations, high productivity, fertility, desired flavoring, aromatic, nourishing,

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Martinez-Picado, Javier, Lorraine Sutton, Maria Pia De Pasquale, Anu V. Savara, and Richard T. D’Aquila. "Human Immunodeficiency Virus Type 1 Cloning Vectors for Antiretroviral Resistance Testing." Journal of Clinical Microbiology 37, no. 9 (1999): 2943–51. http://dx.doi.org/10.1128/jcm.37.9.2943-2951.1999.

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Better detection of minority human immunodeficiency virus type 1 (HIV-1) populations containing gene mutations may improve the usefulness of antiretroviral resistance testing for clinical management. Molecular cloning of HIV-1 PCR products which might improve minority detection can be slow and difficult, and commercially available recombinant virus assays test drug susceptibility of virus pools. We describe novel plasmids and simple methods for rapid cloning of HIV-1 PCR products from patient specimens and their application to generate infectious recombinant virus clones for virus phenotyping

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Taylor, Stephen J. C., Rob C. Brown, Phil A. Keene, and Ian N. Taylor. "Novel screening methods—the key to cloning commercially successful biocatalysts." Bioorganic & Medicinal Chemistry 7, no. 10 (1999): 2163–68. http://dx.doi.org/10.1016/s0968-0896(99)00146-7.

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Liang, Yaping, Yu Zhang, and Liangwei Liu. "Intra-Molecular Homologous Recombination of Scarless Plasmid." International Journal of Molecular Sciences 21, no. 5 (2020): 1697. http://dx.doi.org/10.3390/ijms21051697.

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Although many methods have been reported, plasmid construction compromises transformant efficiency (number of transformants per ng of DNAs) with plasmid accuracy (rate of scarless plasmids). An efficient method is two-step PCR serving DNA amplification. An accurate method is ExnaseII cloning serving homology recombination (HR). We combine DNA amplification and HR to develop an intra-molecular HR by amplifying plasmid DNAs to contain homology 5′- and 3′-terminus and recombining the plasmid DNAs in vitro. An example was to construct plasmid pET20b-AdD. The generality was checked by constructing

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Hussain, Mushtaq, and Joanna B. Wilson. "ID:2026 Investigating Molecular Interactions of EBV Oncogene, EBNA1, by Heterologous Cloning and Peptide Array System." Biomedical Research and Therapy 4, S (2017): 57. http://dx.doi.org/10.15419/bmrat.v4is.267.

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Background: EBNA1 is an important latent genes that is involved in the oncogenesis associated with Epstein Barr Virus. The protein is highly promiscuous in terms of its interaction with the several proteins including EBP2 and USP7, a p53 stabilizer. Herein, we aim to investigate the interactions points of molecular complexes of EBNA1, an EBV oncogene, in order to design potential therapeutic peptides. Methods: Full length EBNA1, EBP2 and USP7 models were constructed employing iterative threading. After proper refinements of models, EBNA1 dimer was constructed employing geometrical and st

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Dissertations / Theses on the topic "Cloning, Molecular – methods"

1

Briju, Betsy J. "Progress of Work towards Cloning Gravity Persistence Signal (gps) Mutants by PCR-Based Methods and Positional Mapping." Ohio University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1320862556.

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Soyler, Alper. "Development Of A Pcr-based Specific Method For The Detection Of Aspergillus Fumigatus By Random Cdna Cloning." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/12605105/index.pdf.

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Aspergillus fumigatus is a foodborne and airborne human pathogen which produces mycotoxins such as gliotoxin, helvolic acid, fumigallin, and fumigaclavin. The most common disease caused by A. fumigatus is aspergillosis, which is often fatal, especially among AIDS, cancer, and organ-transplant patients. In this study, random cDNA cloning was performed from a cDNA library of A. fumigatus (IMI 385708) constructed on &amp<br>#955<br>ZAP Express. Some of these clones were selected according to their insert sizes and were further subjected to sequence analysis. The sequences were then analyzed by a

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Leriche, Françoise. "Recherche d'outils, génétiques utilisables chez la bactérie psycrotrophe MFO, étude de leur comportement aux différentes températures de croissance de la souche et construction d'une fusion traductionnelle par génétique réverse." Rouen, 1993. http://www.theses.fr/1993ROUES040.

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Recherche d'outils génétiques utilisables chez la bactérie pseudomonas fluorescens MFO et mise au point des conditions de leur utilisation aux différentes températures de croissance de la souche. Mise au point d'un vecteur de clonage, de conditions d'intégration de gènes étrangers dans le chromosome bactérien, d'une méthode de cartographie par transfert chromosomique et de transposition par génétique réverse

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Alliotte, Thierry. "Contribution a l'etude de l'expression genetique chez la plante : identification et caracterisation de quatre genes presentant un profil d'expression remarquable." Paris 7, 1988. http://www.theses.fr/1988PA077001.

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Mesnard, Jean-Michel. "Le gene iii du virus de la mosaique du chou-fleur (camv)." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13148.

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BUFFARD, DOMINIQUE. "Contribution a l'etude des relations legumineuses-rhizobium : structure des genes de lectine de pois et analyse de leur expression lors de l'etablissement de la symbiose." Paris 7, 1987. http://www.theses.fr/1987PA077097.

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La sequence et l'organisation des genes de lectine de pois ont ete etudiees afin de pouvoir analyser leur expression et la regulation de cette expression au cours du developpement de la plante. Des sondes specifiques (cdna) de lectines de graines de pois, haricot et soja ont ete utilisees pour detecter les sequences lectiniques dans les genomes de pois, luzerne, arachide et sesbiano restrata. Les 4 fragments ecori du dna genomique de pois hybridant avec le cdna homologue ont ete clones et sequences. Un seul correspond a un gene de lectine fonctionnel, les 3 autres representant des pseudogenes.

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Prost, Edouard. "I : analyse structurale de la famille multigenique de l'hormone de croissance humaine. ii: etude de l'expression proteique du gene k10 implique dans le developpement dorso-ventral de l'oeuf de drosophile." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13136.

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Nieto, Sara. "Inserting dCas9 and single-guide RNAs into Drosophila using molecular cloning methods." Thesis, 2020. https://hdl.handle.net/2144/41312.

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Non-coding DNA in the human genome is widely studied to investigate its effect on coding DNA and gene expression. Non-coding DNA contains cis-regulatory elements that influence transcription of genes upstream, downstream, or nearby. These regulatory elements have largely been studied as enhancers that promote the transcription of genes. To explore these regulatory elements as silencers, we chose validated bifunctional elements to study their silencing capability and their chromatin markers. We used chromatin immunoprecipitation methods with dCas9 to target these elements using single-guide RN

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"Molecular studies of HBV-induced hepatocellular carcinoma by suppression subtractive hybridization and cDNA microarray analyses." 2002. http://library.cuhk.edu.hk/record=b5891149.

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by Shuk-kei Lau.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2002.<br>Includes bibliographical references (leaves 141-148).<br>Abstracts in English and Chinese.<br>Acknowledgement --- p.i<br>Table of Contents --- p.ii<br>Abstract --- p.vi<br>論文摘要 --- p.viii<br>Abbreviations --- p.ix<br>List of Figures --- p.x<br>List of Tables --- p.xii<br>Chapter Chapter 1 --- Introduction<br>Chapter 1.1 --- General introduction --- p.1<br>Chapter 1.2 --- HBV and its role in hepatocarcinogenesis --- p.3<br>Chapter 1.2.1 --- Current situation of HBV infection and the HCC incidencein the worl

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Books on the topic "Cloning, Molecular – methods"

1

A, Lund Peter, and Minchin Steve, eds. Gene cloning. Taylor & Francis Group, 2007.

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Damis, Yancopoulos George, and Alt Frederick W, eds. Methods for cloning and analysis of eukaryotic genes. Jones and Bartlett Publishers, 1990.

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3

Bothwell, Alfred. Methodsfor cloning and analysis of eukaryotic genes. Jones and Bartlett, 1990.

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Spangler, Brenda D. Methods in molecular biology and protein chemistry: Cloning and characterization of an enterotoxin subunit. Wiley, 2002.

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A, Fuchs James, and Messing Joachim W, eds. An introduction to recombinant DNA techniques: Basic experiments in gene manipulation. 2nd ed. Benjamin/Cummings, 1988.

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G, Williams J. Genetic engineering. BIOS Scientific Publishers, 1993.

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K, Patient R., ed. Genetic engineering. IRL Press, 1988.

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A, Ceccarelli, and Wallace A. 1963-, eds. Genetic engineering. 2nd ed. Bios, 2001.

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9

Lodge/Lund/Minc. Gene Cloning. Taylor & Francis, 2006.

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Dna Cloning And Assembly Methods. Humana Press Inc., 2014.

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Book chapters on the topic "Cloning, Molecular – methods"

1

Fontes, Andrew. "Cloning Technologies." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-348-0_20.

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Ohara, Osamu. "ORFeome Cloning." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-232-2_1.

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Gulcher, Jeffrey, and Kari Stefansson. "Positional Cloning." In Methods in Molecular Medicine™. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-159-8_10.

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Li, Duanxiang. "Positional Cloning." In Methods in Molecular Medicine™. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-159-8_9.

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Cortes, Mauricio, James R. Mensch, Miriam Domowicz, and Nancy B. Schwartz. "Proteoglycans: Gene Cloning." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-498-8_1.

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Ro, Seungil, and Wei Yan. "Small RNA Cloning." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-657-3_17.

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Blommel, Paul G., Peter A. Martin, Kory D. Seder, Russell L. Wrobel, and Brian G. Fox. "Flexi Vector Cloning." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-196-3_4.

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Liu, Ling. "Nuclear Transfer and Cloning." In Methods in Molecular Biology. Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9837-1_10.

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Aitman, Timothy J., Enrico Petretto, and Jacques Behmoaras. "Genetic Mapping and Positional Cloning." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-389-3_2.

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Trounson, Alan O. "Future and Applications of Cloning." In Methods in Molecular Biology. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-154-3_22.

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Conference papers on the topic "Cloning, Molecular – methods"

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Wicki, A. N., A. Walz, and K. J. Clemetson. "IDENTIFICATION OF GLYCOPROTEIN lb IN “IN VITRO” TRANSLATES FROM ISOLATED HUMAN PLATELET mRNA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643628.

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Platelet membrane glycoproteins play a crucial role in platelet adhesion and activation. To understand how they function it is of great importance to know their amino acid sequences and structures. It is rather difficult to purify membrane glycoproteins in amounts that are sufficient to determine their amino acid sequences by protein sequencing techniques. The easier way seems to be molecular cloning of the genes for these proteins.Metabolically stable mRNA derived from nucleated megakaryocytes is known to be present in the anuclear human platelets. We have developed a purification method for

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Morrissey, J. H., D. S. Fair, and T. S. Edgington. "STRUCTURE AND PROPERTIES OF THE HUMAN TISSUE FACTOR APOPROTEIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643738.

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Tissue factor (TF), an integral membrane glycoprotein, is an initiating molecule for the coagulation protease cascade. TF must reside in a phospholipid membrane for optimal activity where it functions as the receptor and essential allosteric activator for factor Vll/VIIa.TF apoprotein was purified from human brain and placenta using factor Vll-affinity chromatography or immunoaffinity chromatography with a mouse anti-TF monoclonal antibody. Both methods resulted in a homogeneous preparation consisting of a highly glycosylated 47 kDa heavy chain and a 12.5 kDa light chain.Removal of asparagine-

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Reports on the topic "Cloning, Molecular – methods"

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Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences. Office of Scientific and Technical Information (OSTI), 2001. http://dx.doi.org/10.2172/781022.

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Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences. Office of Scientific and Technical Information (OSTI), 2000. http://dx.doi.org/10.2172/827425.

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Robb, F. T. Molecular profiling of microbial communities from contaminated sources: Use of subtractive cloning methods and rDNA spacer sequences. 1998 annual progress report. Office of Scientific and Technical Information (OSTI), 1998. http://dx.doi.org/10.2172/13700.

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Molecular profiling of microbial communities from contaminated sources: Use of substractive cloning methods and rDNA spacer sequences. 1997 annual progress report. Office of Scientific and Technical Information (OSTI), 1997. http://dx.doi.org/10.2172/13699.

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