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Relevant bibliographies by topics / Cloning vector

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Academic literature on the topic 'Cloning vector'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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Journal articles on the topic "Cloning vector"

1

Li, Ji Gang, Guo Ying Han, Xiu Min Li, Jiao Jiao Sun, Ke Jing Song, and Ting Zhang. "Improvement of TA Cloning Method to Facilitate Direct Directional Cloning of PCR Products." Applied Mechanics and Materials 565 (June 2014): 3–8. http://dx.doi.org/10.4028/www.scientific.net/amm.565.3.

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Directional cloning is a prerequisite for the construction of expression vectors in molecular biology laboratories. Although TA cloning is widely used to clone unmodified PCR (polymerase chain reaction) products, a major disadvantage of this technique is that cloning is not directional. Here we reported a novel PCR products cloning vector with one deoxythymidine overhang and one deoxycytidine overhang at two 3'-ends respectively. With the choice of nucleotides of 5'-ends of PCR primers, PCR products can be cloned to this vector both directly and directionally. The feasibility and efficacy of this cloning method were confirmed by using a pET-17b derivative vector and a green fluorescent protein gene (EGFP) and a red fluorescent protein reporter (Ds-Red) gene. This cloning strategy may be useful in the high-throughput construction of expression vectors and could be viewed as an interesting improvement of existing TA cloning method.

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Dombrecht, Bruno, Jos Vanderleyden, and Jan Michiels. "Stable RK2-Derived Cloning Vectors for the Analysis of Gene Expression and Gene Function in Gram-Negative Bacteria." Molecular Plant-Microbe Interactions® 14, no. 3 (March 2001): 426–30. http://dx.doi.org/10.1094/mpmi.2001.14.3.426.

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The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoter-less gusA gene are suitable for the study of gene function or regulation in bacteria.

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Dobrijevic, Dragana, Lily A. Nematollahi, Helen C. Hailes, and John M. Ward. "pET expression vector customized for efficient seamless cloning." BioTechniques 69, no. 5 (November 2020): 384–87. http://dx.doi.org/10.2144/btn-2020-0101.

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Here we present a modification of the widely used pET29 expression vector for use in rapid and straightforward parallel cloning via a gene replacement and Golden Gate strategy. The modification can be applied to other expression vectors for Gram-negative bacteria. We have used the modified vectors to clone large numbers of bacterial natural enzyme variants from genomic and metagenomic sources for applications in biocatalysis.

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Yao, Wensheng, Yunliu Yang, and Juishen Chiao. "Cloning vector system forNocardia spp." Current Microbiology 29, no. 4 (October 1994): 223–27. http://dx.doi.org/10.1007/bf01570158.

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Wang, Sheng, Haiying Xing, Chenlei Hua, Hui-Shan Guo, and Jie Zhang. "An Improved Single-Step Cloning Strategy Simplifies the Agrobacterium tumefaciens-Mediated Transformation (ATMT)-Based Gene-Disruption Method for Verticillium dahliae." Phytopathology® 106, no. 6 (June 2016): 645–52. http://dx.doi.org/10.1094/phyto-10-15-0280-r.

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The soilborne fungal pathogen Verticillium dahliae infects a broad range of plant species to cause severe diseases. The availability of Verticillium genome sequences has provided opportunities for large-scale investigations of individual gene function in Verticillium strains using Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene-disruption strategies. Traditional ATMT vectors require multiple cloning steps and elaborate characterization procedures to achieve successful gene replacement; thus, these vectors are not suitable for high-throughput ATMT-based gene deletion. Several advancements have been made that either involve simplification of the steps required for gene-deletion vector construction or increase the efficiency of the technique for rapid recombinant characterization. However, an ATMT binary vector that is both simple and efficient is still lacking. Here, we generated a USER-ATMT dual-selection (DS) binary vector, which combines both the advantages of the USER single-step cloning technique and the efficiency of the herpes simplex virus thymidine kinase negative-selection marker. Highly efficient deletion of three different genes in V. dahliae using the USER-ATMT-DS vector enabled verification that this newly-generated vector not only facilitates the cloning process but also simplifies the subsequent identification of fungal homologous recombinants. The results suggest that the USER-ATMT-DS vector is applicable for efficient gene deletion and suitable for large-scale gene deletion in V. dahliae.

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Gardner, Warren L., and William B. Whitman. "Expression Vectors for Methanococcus maripaludis: Overexpression of Acetohydroxyacid Synthase and β-Galactosidase." Genetics 152, no. 4 (August 1, 1999): 1439–47. http://dx.doi.org/10.1093/genetics/152.4.1439.

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Abstract A series of integrative and shuttle expression vectors was developed for use in Methanococcus maripaludis. The integrative expression vectors contained the Methanococcus voltae histone promoter and multiple cloning sites designed for efficient cloning of DNA. Upon transformation, they can be used to overexpress specific homologous genes in M. maripaludis. When tested with ilvBN, which encodes the large and small subunits of acetohydroxyacid synthase, transformants possessed specific activity 13-fold higher than that of the wild type. An expression shuttle vector, based on the cryptic plasmid pURB500 and the components of the integrative vector, was also developed for the expression of heterologous genes in M. maripaludis. The β-galactosidase gene from Escherichia coli was expressed to ∼1% of the total cellular protein using this vector. During this work, the genes for the acetohydroxyacid synthase (ilvBN) and phosphoenolpyruvate synthase (ppsA) were sequenced from a M. maripaludis genomic library.

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Yoshihama, M., K. Higashiro, E. A. Rao, M. Akedo, W. G. Shanabruch, M. T. Follettie, G. C. Walker, and A. J. Sinskey. "Cloning vector system for Corynebacterium glutamicum." Journal of Bacteriology 162, no. 2 (1985): 591–97. http://dx.doi.org/10.1128/jb.162.2.591-597.1985.

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Chauthaiwale, V. M., A. Therwath, and V. V. Deshpande. "Bacteriophage lambda as a cloning vector." Microbiological Reviews 56, no. 4 (1992): 577–91. http://dx.doi.org/10.1128/mmbr.56.4.577-591.1992.

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Chauthaiwale, V. M., A. Therwath, and V. V. Deshpande. "Bacteriophage lambda as a cloning vector." Microbiological Reviews 56, no. 4 (1992): 577–91. http://dx.doi.org/10.1128/mr.56.4.577-591.1992.

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Tan, Lendl, Emily J. Strong, Kyra Woods, and Nicholas P. West. "Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method." PeerJ 6 (June 29, 2018): e5146. http://dx.doi.org/10.7717/peerj.5146.

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Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min. HAC facilitates insert integration due to a sequence alignment strategy, by way of short, vector-specific homology tails appended to insert during amplification. Simultaneous exposure of single-stranded fragment ends, utilising the 3′→5′ exonuclease activity of T4 DNA polymerase, creates overlapping homologous DNA on each molecule. The exonuclease activity of T4 polymerase is quenched simply by the addition of EDTA and a simple annealing step ensures high yield and high fidelity vector formation. The resultant recombinant plasmids are transformed into standardE. colicloning strains and screened via established methods as necessary. HAC exploits reagents commonly found in molecular research laboratories and achieves efficiencies that exceed conventional cloning methods, including another ligation-independent method we tested. HAC is also suitable for combining multiple fragments in a single reaction, thus extending its flexibility.

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Dissertations / Theses on the topic "Cloning vector"

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Shaw, Pang Chui. "A host-vector system for an Arthrobacter species and cloning of its glucose isomerase gene." Thesis, Imperial College London, 1987. http://hdl.handle.net/10044/1/46538.

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Räsänen, Kati. "Development of a Transfection System for the Free-Living Amoeba Naegleria fowleri Using the piggyBac Vector." Scholar Commons, 2017. http://scholarcommons.usf.edu/etd/6751.

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Naegleria fowleri is a free-living amoeba that causes primary amoebic meningoencephalitis (PAM). In the United States, there are between 0-8 cases of PAM per year, with approximately 98% of cases resulting in death. High case fatality and limited treatment options highlight the need for better understanding of this organism in terms of its biology and pathogenicity. Transfection is a useful tool that allows for the study of gene function, but at present no transfection systems have been established for N. fowleri. This study attempts to establish a transfection system for N. fowleri using the piggyBac vector, with the hope of eventually using the piggyBac transposon system to identify novel genes related to pathogenicity in N. fowleri. To accomplish this, 5’ and 3’ regulatory regions for genes in the N. fowleri genome were amplified and inserted into a piggyBac vector with a GFP reporter gene via molecular cloning, and vectors introduced to the amoeba via electroporation. Although no GFP was visualized after transfection, there are several routes for optimization of the transfection system that could be explored. Development of a transfection system could allow for the study of pathogenicity in vivo, by either utilizing the transposon system of piggyBac or the expression of reporter genes for visualization of amoeba during the course of infection. Further elucidating N. fowleri pathogenicity factors could reveal new drug targets, give new information about the organism’s biology, and help better define an effective treatment regimen to combat PAM.

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Helvering, Leah M. "Cloning of genes encoding larvicidal proteins from Bacillus thuringiensis subsp. israelensis into the cyanobacterial hybrid vector, pTNTV." Virtual Press, 1989. http://liblink.bsu.edu/uhtbin/catkey/562782.

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Bacillus thuringiensis subsp. isrealensis (B.t.i.) produces a crystalline endotoxin specific for some larvae of mosquitoes that are vectors of the malaria parasite and other infectious diseases. Fragments were obtained from the 108 kb plasmid from B.t.i. strain 4Q2 which encodes several proteins comprising the delta-endotoxin. These DNA fragments were inserted into the hybrid cyanobacterial cloning vector, pTNTV, downstream from its powerful lambda promoter, and the chimaeras were transformed into Escherichia coli. Ampicillin resistant transformants were screened with radioactively labelled oligonucleotides whose sequences were determined from the published sequences of the B.t.i. 130 kDa polypeptide. Clones showing hybridization were used in bioassays to determine their level of toxicity to the fourth instar larvae of the Aedes aegypti mosquito. Twelve clones were found that demonstrated toxicity which was statistically significantly greater than that observed in controls. Plasmid DNA from some of these clones was isolated, cut with restriction endonucleases, and viewed through agarose gel electrophoresis to confirm that B.t.i. fragments had been inserted into the vector. Future work will investigate the expression of these cloned toxin genes in transformable cyanobacteria and will determine their subsequent activity against the fourth instar larvae of Aedes aegypti and Anopheles quadrimaculatus.
Department of Biology

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Frank, Sander B., Veronique V. Schulz, and Cindy K. Miranti. "A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector." BIOMED CENTRAL LTD, 2017. http://hdl.handle.net/10150/623280.

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Background: Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of proliferation state. Furthermore, the use of inducible promoters to drive shRNA expression allows for more thorough investigations into the specific timing of gene function in a variety of cellular processes. Moreover, inducible knockdown allows the investigation of genes that would be lethal or otherwise poorly tolerated if constitutively knocked down. Lentiviral inducible shRNA vectors are readily available, but unfortunately the process of cloning, screening, and testing shRNAs can be time-consuming and expensive. Therefore, we sought to refine a popular vector (Tet-pLKO-Puro) and streamline the cloning process with efficient protocols so that researchers can more efficiently utilize this powerful tool. Methods: First, we modified the Tet-pLKO-Puro vector to make it easy("EZ") for molecular cloning (EZ-Tet-pLKO-Puro). Our primary modification was to shrink the stuffer region, which allows vector purification via polyethylene glycol precipitation thereby avoiding the need to purify DNA through agarose. In addition, we generated EZ-Tet-pLKO vectors with hygromycin or blasticidin resistance to provide greater flexibility in cell line engineering. Furthermore, we provide a detailed guide for utilizing these vectors, including shRNA design strategy and simplified screening methods. Results: Notably, we emphasize the importance of loop sequence design and demonstrate that the addition of a single mismatch in the loop stem can greatly improve shRNA efficiency. Lastly, we display the robustness of the system with a doxycycline titration and recovery time course and provide a cost/benefit analysis comparing our system with purchasing pre-designed shRNA vectors. Conclusions: Our aim was twofold: first, to take a very useful shRNA vector and make it more amenable for molecular cloning and, secondly, to provide a streamlined protocol and rationale for cost-effective design, cloning, and screening of shRNAs. With this knowledge, anyone can take advantage of this powerful tool to inducibly knockdown any gene of their choosing.

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Bae, Insoo. "Construction of a hybrid vector which allows for temperature regulation of expression of cloned genes in cyanobacterium, Synechocystis 6803." Virtual Press, 1988. http://liblink.bsu.edu/uhtbin/catkey/544002.

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A hybrid vector, pBVl, has been constructed which is capable of regulating expression of cloned genes in both Escherichia coli and in cyanobacterium Synechocystis 6803. Vector pBVl contains the powerful rightward promoter of bacteriophage lambda to ensure that cloned genes are transcibed at a high level. In addition, pBV] also contains a gene, cI857, encoding the lambda temperature sensitive repressor protein.The CAT gene coding for chloramphenicol aceyltransferase (CmActase) was cloned into pBV1 downstream of the lambda regulatory features to make plasmid pTC1. The expression of the CAT gene was quantified spectrophotometrically following addition of chloramphenicol and a shift in the growth temperature of the cell culture. The specific activity of CmActase increased from 540 to 5400 units within 30 minutes. Thus, using the newly constructed hybrid vector, pBVl, temperature regulation of gene expression in E. coli was observed.
Department of Biology

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Summers, Phillip Jared. "Cloning of Manduca sexta Serotonin Receptors 5HT1a, 5HT1b, and 5HT7 Into Xenopus laevis Oocyte Expression Vector PBS-MXT." Thesis, The University of Arizona, 2009. http://hdl.handle.net/10150/192963.

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Jeong, Pyengsoo. "Construction of a Cloning Vector Based upon a Rhizobium Plasmid Origin of Replication and its Application to Genetic Engineering of Rhizobium Strains." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc278189/.

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Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia as symbiont bacteroids within nodules of leguminous plant roots. Here, resident Rhizobium plasmids were studied as possible sources of components for the construction of a cloning vector for Rhizobium species.

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Lloyd, Amanda Lian. "Cloning, characterisation and sequencing of promoters of Helicobacter pylori 4187E." University of Western Australia. Microbiology Discipline Group, 2005. http://theses.library.uwa.edu.au/adt-WU2005.0112.

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Published information on the structure and regulation of H. pylori promoters is limited. The work presented in this thesis describes the cloning and characterisation of promoter regions from a clinical isolate of H. pylori, and the development of an alternative, non-radioactive method for verifying the location of transcriptional start sites of bacterial promoters. H. pylori 4187E promoters were randomly cloned into the promoter-trap vector pKK232-8 in Escherichia coli DH5α using two sets of restriction enzymes. Vector pKK232-8 contains a promoterless chloramphenicol acetyltransferase (CAT) gene. Seventy-four promoter-containing clones were isolated from selective media based on their resistance to chloramphenicol. The strength of each promoter was analysed qualitatively, using chloramphenicol minimum inhibitory concentrations, and quantitatively, using CAT assays following exposure of the clones to pH 4 and pH 7. Selected promoter fragments were subcloned into the GFP reporter vector pFPV25, containing a promoterless gfp gene. The subclones were exposed to buffered LB broth at pH 4, 5, 6, 7 and 8, for varying lengths of time, to study acid-induced regulation of gene expression. Subclones were examined qualitatively, using visual examination of GFP fluorescence and fluorescence microscopy, and quantitatively, using flow cytometry following acid shock. DNA sequences were determined for 61 of the 74 H. pylori promoters, and sequence alignments with the published H. pylori strains (26695 and J99) were performed. The transcriptional start site of 27 H. pylori promoter fragments was experimentally mapped using a fluorescence-based primer extension protocol developed by our group. Potential -35 and -10 sequences were identified for each promoter, and a new consensus sequence for H. pylori promoters was proposed based upon these results. This study has considerably expanded knowledge of H. pylori promoter sequences and transcriptional start sites based on those which also function in E. coli. It has also revealed several H. pylori promoters which appear to respond to acid stress

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Snyder, William E. "Construction of a hybrid vector which allows for regulation of expression of cloned genes in anacystis nidulans R2 by controlling the iron content of the growth medium." Virtual Press, 1989. http://liblink.bsu.edu/uhtbin/catkey/560278.

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A hybrid vector, pANIC1, was to be constructed which was capable of regulating expression of cloned genes in both Escherichia coli and Anacystis nidulans R2 by controlling the iron content of the growth medium. Plasmid pANIC1 would have origins of replication for E. coli and A. nidulans R2, and a marker gene conferring ampicillin resistance. It would also contain the promoter for the irpA gene which is active only in low iron growth conditions.The first two stages of the construction were successfully completed, but unfortunately the final construction proved to be unstable. Recent information has shown that operator sequences upstream from the irpA gene's promoter result in an unstable message. This may be interfering with the normal functioning of the host cell, resulting in an unstable construction. In future experiments it may be neccessary to alter the growth conditions or remove the upstream sequences in order to stablize the construction.
Department of Biology

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Chukwurah, George A. "Development of a stable expression vector system for gene transfer into sceletal muscle followinq detection of sequence instability in utrophin cDNA during cloning." Thesis, Royal Holloway, University of London, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521777.

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Books on the topic "Cloning vector"

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Sharan, Niki. Cloning of the marine apoptosis inducer, interleukin-1B converting enzyme (ICE), into a yeast expression vector. Sudbury, Ont: Laurentian University, 1996.

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2

E, Enger-Valk B., and Brammar W. J, eds. Cloning vectors. Oxford: Elsevier, 1987.

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Pouwels, P. H. Cloning vectors: A laboratory manual. Amsterdam: Elsevier, 1985.

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Brown, Antony. Broad-host-range cloning vectors and gene expression systems. Birmingham: University of Birmingham, 1990.

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Heale, S. M. Factors effecting the utility of yeast artificial chromosomes as cloning vectors. Manchester: UMIST, 1993.

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Sit, Timmy Lawrence *. Cloning of the genome of papaya mosaic virus in construction of a potential shuttle vector. 1988.

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P, Jones, ed. Vectors: Cloning applications. Chichester: Wiley, 1998.

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Jones, P. Vectors: Cloning Applications. Wiley & Sons, Incorporated, John, 2009.

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Li, Guoxuan. The characterization and cloning of the RNA of a vector-nonspecific isolate of barley yellow dwarf virus commonly found in wheat in Washington State. 1990.

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10

H, Pouwels P., Enger-Valk B. E, and Brammar W. J, eds. Cloning vectors: A laboratory manual. Oxford: Elsevier, 1985.

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Book chapters on the topic "Cloning vector"

1

Julin, Douglas A. "Cloning Vector Compatibility." In Molecular Life Sciences, 106–8. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4614-1531-2_92.

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Blommel, Paul G., Peter A. Martin, Kory D. Seder, Russell L. Wrobel, and Brian G. Fox. "Flexi Vector Cloning." In Methods in Molecular Biology, 55–73. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-196-3_4.

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Julin, Douglas. "Cloning Vector Compatibility." In Molecular Life Sciences, 1–2. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4614-6436-5_92-4.

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Wong, Dominic W. S. "Gene-Vector Construction." In The ABCs of Gene Cloning, 123–29. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-77982-9_10.

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Mutzel, Rupert. "λ as a Cloning Vector." In Springer Protocols Handbooks, 153–63. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_15.

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Huang, Hongyun, and Ning Du. "Cana Cloning and Plant Expression Vector Construction of Soybean 11s Glycerin Gy3." In Lecture Notes in Electrical Engineering, 237–44. London: Springer London, 2013. http://dx.doi.org/10.1007/978-1-4471-4850-0_31.

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Deichmann, Annette, and Klaus Deichmann. "Cloning Vectors." In Techniques in Molecular Medicine, 226–54. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_15.

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Julin, Douglas A. "Plasmid Cloning Vectors." In Molecular Life Sciences, 925–35. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4614-1531-2_86.

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Julin, Douglas. "Plasmid Cloning Vectors." In Molecular Life Sciences, 1–12. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-6436-5_86-1.

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DiMaio, Daniel. "Papillomavirus Cloning Vectors." In The Papovaviridae, 293–319. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4757-0584-3_11.

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Conference papers on the topic "Cloning vector"

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Wang, Xiran, Leiyu Jiang, and Haoru Tang. "Cloning and Construction of Overexpression Vector for FaUVR8 Gene Transformation with Strawberry." In 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/ifeesm-17.2018.350.

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Cong Li, Tianqi Guo, Feifei Li, Yongxia Bao, and Liebao Han. "Cloning of DREB gene from Lilyturf (Ophiopogon japonicus) and construction of a plant expression vector." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965893.

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Uzan, G., A. Lajmanovich, M. H. Prandini, Ph Frachet, A. Duperray, and G. Marguerie. "MOLECULAR CLONING OF PLATELET GPIIb FROM HEL CELLS AND HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643960.

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Platelet GP IIb-IIIa is an heterodimer which functions as a receptor for fibrinogen, fibronectin and Von Willebrand factor and is implicated in platelet adhesive reactions. To study the structure function relationship of this glycoprotein, a recombinant DNA approach was initiated. cDNA expression libraries were constructed in » gtll vector, from erythro-leukemia cells (HEL) and megakaryocytes mRNA. The human megakaryocytes were isolated from patients with chronic myeloid leukemia. The HEL library was initially screened with polyclonal antibodies anti GPIIb IIIa. One clone, λIIbI, containing a 1.65 kbp insert reacted with a panel of different polyclonal antibodies anti GPIIb IIIa and a monoclonal antibody anti GPIIb. To further characterize this clone the synthesis of the fusion protein was induced by IPTG. The bacterial protein was then blotted onto nitro cellulose and incubated with antisera anti GPIIb-IIIa. Antibodies that specifically bound with the fusion protein were eluted and tested on platelet membrane extracts. The selected antibodies produced a positive signal at the GPIIb position similar to the signal produced by the monoclonal antibody anti GPIIb on the same membrane extract. Finally on western blotting, a protein of Mr= 170kD reacted with the monoclonal antibody anti GPIIb. λIIbI insert was used to screen the megakaryocyte library and 3 clones, λIIb2,λIIb3 and λIIb4 were isolated. The size of HEL cells and megakaryocytes GPIIb mRNA was estimated by northern blotting. Only one species of 3.9 kb was identified in both cells. The four different clones accounted for 50% of the coding sequence of this mRNA.Sequencing of these cDNAs indicated that the plasmatic domain of GPIIb contains a cystein rich region. The sequence of these clones will allow the study of the adhesines genetic diversity in different cellular systems.

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Subiantistha, T., S. Pambudi, A. F. Rahmani, S. A. Puteri, and R. Lestari. "Cloning of recombinant fab from monoclonal antibody anti-dengue NS1 induced by recombinant CHO-K1 cells into pGEM-T vector." In PROCEEDINGS OF THE 4TH INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES (ISCPMS2018). AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5132532.

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Muammar, Arief, Suci Aulia Ratu Fajrin, and Endah Retnaningrum. "Cellobiohydrolase A (CBHA) gene cloning from Aspergillus niger to the yeast expression vector as a stages to create cellulosic ethanol strain." In THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity”. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0016145.

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Panfilov, A. V., Yu M. Konstantinov, and M. V. Koulintchenko. "THE OPTIMIZATION OF CLONING OF LONG-SIZED DNA FRAGMENTS IN p-GEM VECTOR, CONTAINING TERMINAL INVERTED REPEATS OF LINEAR MITOCHONDRIAL PLASMIDS FROM ZEA MAYS." In The Second All-Russian Scientific Conference with international participation "Regulation Mechanisms of Eukariotic Cell Organelle Functions". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-318-1-84-86.

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Rahmani, A. F., S. Pambudi, S. A. Puteri, T. Subiantistha, and R. Lestari. "Cloning of the heavy chain of fragment antigen binding anti-NS1 from hybridoma cell 71E2 induced by dengue virus on pTA2 vector in Escherichia coli TOP10." In PROCEEDINGS OF THE 4TH INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES (ISCPMS2018). AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5132531.

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Reports on the topic "Cloning vector"

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(New hosts and vectors for genome cloning). Office of Scientific and Technical Information (OSTI), January 1991. http://dx.doi.org/10.2172/5603523.

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(New hosts and vectors for genome cloning). Office of Scientific and Technical Information (OSTI), January 1991. http://dx.doi.org/10.2172/5822496.

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[New hosts and vectors for genome cloning]. Progress report. Office of Scientific and Technical Information (OSTI), December 1991. http://dx.doi.org/10.2172/10118257.

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[New hosts and vectors for genome cloning]. Progress report, 1990--1991. Office of Scientific and Technical Information (OSTI), December 1991. http://dx.doi.org/10.2172/10116373.

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