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Journal articles on the topic 'Cloning vector'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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1

Li, Ji Gang, Guo Ying Han, Xiu Min Li, Jiao Jiao Sun, Ke Jing Song, and Ting Zhang. "Improvement of TA Cloning Method to Facilitate Direct Directional Cloning of PCR Products." Applied Mechanics and Materials 565 (June 2014): 3–8. http://dx.doi.org/10.4028/www.scientific.net/amm.565.3.

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Directional cloning is a prerequisite for the construction of expression vectors in molecular biology laboratories. Although TA cloning is widely used to clone unmodified PCR (polymerase chain reaction) products, a major disadvantage of this technique is that cloning is not directional. Here we reported a novel PCR products cloning vector with one deoxythymidine overhang and one deoxycytidine overhang at two 3'-ends respectively. With the choice of nucleotides of 5'-ends of PCR primers, PCR products can be cloned to this vector both directly and directionally. The feasibility and efficacy of this cloning method were confirmed by using a pET-17b derivative vector and a green fluorescent protein gene (EGFP) and a red fluorescent protein reporter (Ds-Red) gene. This cloning strategy may be useful in the high-throughput construction of expression vectors and could be viewed as an interesting improvement of existing TA cloning method.

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2

Dombrecht, Bruno, Jos Vanderleyden, and Jan Michiels. "Stable RK2-Derived Cloning Vectors for the Analysis of Gene Expression and Gene Function in Gram-Negative Bacteria." Molecular Plant-Microbe Interactions® 14, no. 3 (March 2001): 426–30. http://dx.doi.org/10.1094/mpmi.2001.14.3.426.

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The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoter-less gusA gene are suitable for the study of gene function or regulation in bacteria.

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3

Dobrijevic, Dragana, Lily A. Nematollahi, Helen C. Hailes, and John M. Ward. "pET expression vector customized for efficient seamless cloning." BioTechniques 69, no. 5 (November 2020): 384–87. http://dx.doi.org/10.2144/btn-2020-0101.

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Here we present a modification of the widely used pET29 expression vector for use in rapid and straightforward parallel cloning via a gene replacement and Golden Gate strategy. The modification can be applied to other expression vectors for Gram-negative bacteria. We have used the modified vectors to clone large numbers of bacterial natural enzyme variants from genomic and metagenomic sources for applications in biocatalysis.

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4

Yao, Wensheng, Yunliu Yang, and Juishen Chiao. "Cloning vector system forNocardia spp." Current Microbiology 29, no. 4 (October 1994): 223–27. http://dx.doi.org/10.1007/bf01570158.

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5

Wang, Sheng, Haiying Xing, Chenlei Hua, Hui-Shan Guo, and Jie Zhang. "An Improved Single-Step Cloning Strategy Simplifies the Agrobacterium tumefaciens-Mediated Transformation (ATMT)-Based Gene-Disruption Method for Verticillium dahliae." Phytopathology® 106, no. 6 (June 2016): 645–52. http://dx.doi.org/10.1094/phyto-10-15-0280-r.

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The soilborne fungal pathogen Verticillium dahliae infects a broad range of plant species to cause severe diseases. The availability of Verticillium genome sequences has provided opportunities for large-scale investigations of individual gene function in Verticillium strains using Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene-disruption strategies. Traditional ATMT vectors require multiple cloning steps and elaborate characterization procedures to achieve successful gene replacement; thus, these vectors are not suitable for high-throughput ATMT-based gene deletion. Several advancements have been made that either involve simplification of the steps required for gene-deletion vector construction or increase the efficiency of the technique for rapid recombinant characterization. However, an ATMT binary vector that is both simple and efficient is still lacking. Here, we generated a USER-ATMT dual-selection (DS) binary vector, which combines both the advantages of the USER single-step cloning technique and the efficiency of the herpes simplex virus thymidine kinase negative-selection marker. Highly efficient deletion of three different genes in V. dahliae using the USER-ATMT-DS vector enabled verification that this newly-generated vector not only facilitates the cloning process but also simplifies the subsequent identification of fungal homologous recombinants. The results suggest that the USER-ATMT-DS vector is applicable for efficient gene deletion and suitable for large-scale gene deletion in V. dahliae.

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6

Gardner, Warren L., and William B. Whitman. "Expression Vectors for Methanococcus maripaludis: Overexpression of Acetohydroxyacid Synthase and β-Galactosidase." Genetics 152, no. 4 (August 1, 1999): 1439–47. http://dx.doi.org/10.1093/genetics/152.4.1439.

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Abstract A series of integrative and shuttle expression vectors was developed for use in Methanococcus maripaludis. The integrative expression vectors contained the Methanococcus voltae histone promoter and multiple cloning sites designed for efficient cloning of DNA. Upon transformation, they can be used to overexpress specific homologous genes in M. maripaludis. When tested with ilvBN, which encodes the large and small subunits of acetohydroxyacid synthase, transformants possessed specific activity 13-fold higher than that of the wild type. An expression shuttle vector, based on the cryptic plasmid pURB500 and the components of the integrative vector, was also developed for the expression of heterologous genes in M. maripaludis. The β-galactosidase gene from Escherichia coli was expressed to ∼1% of the total cellular protein using this vector. During this work, the genes for the acetohydroxyacid synthase (ilvBN) and phosphoenolpyruvate synthase (ppsA) were sequenced from a M. maripaludis genomic library.

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7

Yoshihama, M., K. Higashiro, E. A. Rao, M. Akedo, W. G. Shanabruch, M. T. Follettie, G. C. Walker, and A. J. Sinskey. "Cloning vector system for Corynebacterium glutamicum." Journal of Bacteriology 162, no. 2 (1985): 591–97. http://dx.doi.org/10.1128/jb.162.2.591-597.1985.

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8

Chauthaiwale, V. M., A. Therwath, and V. V. Deshpande. "Bacteriophage lambda as a cloning vector." Microbiological Reviews 56, no. 4 (1992): 577–91. http://dx.doi.org/10.1128/mmbr.56.4.577-591.1992.

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9

Chauthaiwale, V. M., A. Therwath, and V. V. Deshpande. "Bacteriophage lambda as a cloning vector." Microbiological Reviews 56, no. 4 (1992): 577–91. http://dx.doi.org/10.1128/mr.56.4.577-591.1992.

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10

Tan, Lendl, Emily J. Strong, Kyra Woods, and Nicholas P. West. "Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method." PeerJ 6 (June 29, 2018): e5146. http://dx.doi.org/10.7717/peerj.5146.

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Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min. HAC facilitates insert integration due to a sequence alignment strategy, by way of short, vector-specific homology tails appended to insert during amplification. Simultaneous exposure of single-stranded fragment ends, utilising the 3′→5′ exonuclease activity of T4 DNA polymerase, creates overlapping homologous DNA on each molecule. The exonuclease activity of T4 polymerase is quenched simply by the addition of EDTA and a simple annealing step ensures high yield and high fidelity vector formation. The resultant recombinant plasmids are transformed into standardE. colicloning strains and screened via established methods as necessary. HAC exploits reagents commonly found in molecular research laboratories and achieves efficiencies that exceed conventional cloning methods, including another ligation-independent method we tested. HAC is also suitable for combining multiple fragments in a single reaction, thus extending its flexibility.

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11

Klevanskaa, Karina, Nadja Bier, Kerstin Stingl, Eckhard Strauch, and Stefan Hertwig. "PVv3, a New Shuttle Vector for Gene Expression in Vibrio vulnificus." Applied and Environmental Microbiology 80, no. 4 (December 20, 2013): 1477–81. http://dx.doi.org/10.1128/aem.03720-13.

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ABSTRACTAn efficient electroporation procedure forVibrio vulnificuswas designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 106transformants per μg DNA were achieved. The vector stably replicated in bothV. vulnificusandEscherichia coliand was also successfully introduced intoVibrio parahaemolyticusandVibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and thevvhBAhemolysin operon were inserted into the vector and functionally expressed inVibrioandE. coli.

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12

Li, Min, Ling-Yan Jiang, Qin Liu, Yuan-Hang Wu, Guo-Dao Liu, Yin-Hua Chen, and Li-Juan Luo. "A method combining TA cloning and fluorescence screening for rapid acquisition of transgenic seeds." BioTechniques 68, no. 5 (May 2020): 251–56. http://dx.doi.org/10.2144/btn-2019-0141.

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The establishment of transgenic plants has greatly promoted the progress of plant research. However, traditional selection methods using antibiotics or herbicides may miss any positive transformants with growth defects. Additionally, screening with antibiotics/herbicides requires a huge amount of seeds, sterile work conditions and a large amount of space to germinate plants, making the selection process time- and labor-consuming. In this study, we constructed a novel stable transformation vector, plasmid of OLE1-GFP T-DNA vector (pOGT), which can shorten the steps of cloning foreign genes into expression vectors by using TA cloning. Additionally, selection of transformed seeds with fluorescence overcomes the difficulties of conventional selection with antibiotics/herbicides and simplifies the screening process for transgenic plants.

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13

Wong, Wing Yee, Ping Su, Gwen E. Allison, Chun-Qiang Liu, and Noel W. Dunn. "A Potential Food-Grade Cloning Vector for Streptococcus thermophilus That Uses Cadmium Resistance as the Selectable Marker." Applied and Environmental Microbiology 69, no. 10 (October 2003): 5767–71. http://dx.doi.org/10.1128/aem.69.10.5767-5771.2003.

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ABSTRACT A potential food-grade cloning vector, pND919, was constructed and transformed into S. thermophilus ST3-1, a plasmid-free strain. The vector contains DNAs from two different food-approved organisms, Streptococcus thermophilus and Lactococcus lactis. The 5.0-kb pND919 is a derivative of the cloning vector pND918 (9.3 kb) and was constructed by deletion of the 4.3-kb region of pND918 which contained DNA from non-food-approved organisms. pND919 carries a heterologous native cadmium resistance selectable marker from L. lactis M71 and expresses the Cdr phenotype in S. thermophilus transformants. With the S. thermophilus replicon derived from the shuttle vector pND913, pND919 is able to replicate in the two S. thermophilus industrial strains tested, ST3-1 and ST4-1. Its relatively high retention rate in S. thermophilus further indicates its usefulness as a potential food-grade cloning vector. To our knowledge, this is the first report of a replicative potential food-grade vector for the industrially important organism S. thermophilus.

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14

Tett, Adrian J., Steven J. Rudder, Alexandre Bourdès, Ramakrishnan Karunakaran, and Philip S. Poole. "Regulatable Vectors for Environmental Gene Expression in Alphaproteobacteria." Applied and Environmental Microbiology 78, no. 19 (July 20, 2012): 7137–40. http://dx.doi.org/10.1128/aem.01188-12.

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ABSTRACTTwo expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment andin planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

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15

Kendall, Kevin, and John Cullum. "A vector for studying plasmid stability functions in Streptomyces." Genetical Research 51, no. 1 (February 1988): 71–74. http://dx.doi.org/10.1017/s0016672300023971.

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SummaryWe constructed a cloning vector (pMT603) based on the low copy number plasmid SCP2*. pMT6O3 is unstable because it lacks the SCP2* stability region and carries the selectable marker thiostrepton-resistance and a tyrosinase gene which results in melanin production. This allows easy testing of plasmid stability and we demonstrated its usefulness by cloning a plasmid stability function.

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16

Godiska, Ronald, Nikolai V. Ravin, and David A. Mead. "Linear Plasmid Vector for Cloning “Unclonable” DNA." BioTechniques 45, no. 5 (November 2008): 592. http://dx.doi.org/10.2144/000113019.

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17

Nakamura, Jun, Sohei Kanno, Eiichiro Kimura, Kazuhiko Matsui, Tsuyoshi Nakamatsu, and Masaaki Wachi. "Temperature-sensitive cloning vector for Corynebacterium glutamicum." Plasmid 56, no. 3 (November 2006): 179–86. http://dx.doi.org/10.1016/j.plasmid.2006.05.003.

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18

Diderichsen, Børge, Gitte Bak Poulsen, and Steen T. Jørgensen. "A Useful Cloning Vector for Bacillus subtilis." Plasmid 30, no. 3 (November 1993): 312–15. http://dx.doi.org/10.1006/plas.1993.1066.

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19

Glucksman, Marc J., S. Bhattacharjee, and Lee Makowski. "Three-dimensional structure of a cloning vector." Journal of Molecular Biology 226, no. 2 (July 1992): 455–70. http://dx.doi.org/10.1016/0022-2836(92)90960-r.

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20

Quigley, Neil B., and Peter R. Reeves. "Chloramphenicol resistance cloning vector based on pUC9." Plasmid 17, no. 1 (January 1987): 54–57. http://dx.doi.org/10.1016/0147-619x(87)90008-4.

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21

Son, Lo Thanh, Le Van Son, Nguyen Vu Thanh Thanh, and Chu Hoang Mau. "Cloning and Designing Vector Carrying GmEXP1 Gene Isolated from Local Soybean Cultivar Sonla, Vietnam." International Journal of Bioscience, Biochemistry and Bioinformatics 4, no. 3 (2014): 191–94. http://dx.doi.org/10.7763/ijbbb.2014.v4.337.

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22

Dandanell, Gert, Donald L. Court, and Karin Hammer. "A single-copy galK promoter cloning vector suitable for cloning strong promoters." Gene Analysis Techniques 3, no. 6 (November 1986): 102–11. http://dx.doi.org/10.1016/0735-0651(86)90016-6.

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23

Tagliavia, Marcello, and Aldo Nicosia. "Advanced Strategies for Food-Grade Protein Production: A New E. coli/Lactic Acid Bacteria Shuttle Vector for Improved Cloning and Food-Grade Expression." Microorganisms 7, no. 5 (April 27, 2019): 116. http://dx.doi.org/10.3390/microorganisms7050116.

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Food-grade production of recombinant proteins in Gram-positive bacteria, especially in LAB (i.e., Lactococcus, Lactobacillus, and Streptococcus), is of great interest in the areas of recombinant enzyme production, industrial food fermentation, gene and metabolic engineering, as well as antigen delivery for oral vaccination. Food-grade expression relies on hosts generally considered as safe organisms and on clone selection not dependent on antibiotic markers, which limit the overall DNA manipulation workflow, as it can be carried out only in the expression host and not in E. coli. Moreover, many commercial expression vectors lack useful elements for protein purification. We constructed a “shuttle” vector containing a removable selective marker, which allows feasible cloning steps in E. coli and subsequent protein expression in LAB. In fact, the cassette can be easily excised from the selected recombinant plasmid, and the resulting marker-free vector transformed into the final LAB host. Further useful elements, as improved MCS, 6xHis-Tag, and thrombin cleavage site sequences were introduced. The resulting vector allows easy cloning in E. coli, can be quickly converted in a food-grade expression vector and harbors additional elements for improved recombinant protein purification. Overall, such features make the new vector an improved tool for food-grade expression.

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24

Towler, Eric M., Jeffrey W. Stebbins, and Christine Debouck. "A single vector cloning, mutagenesis and expression system." Gene 183, no. 1-2 (January 1996): 259–63. http://dx.doi.org/10.1016/s0378-1119(96)00489-1.

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25

Penfold, Robert J., and J. M. Pemberton. "A transposon vector for broad host range cloning." Nucleic Acids Research 18, no. 19 (1990): 5913. http://dx.doi.org/10.1093/nar/18.19.5913.

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26

Janner, Christiane R., Ana Lívia P. Brito, Lidia Maria P. Moraes, Viviane CB Reis, and Fernando AG Torres. "pPCV, a versatile vector for cloning PCR products." SpringerPlus 2, no. 1 (2013): 441. http://dx.doi.org/10.1186/2193-1801-2-441.

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27

Yanase, Hideshi, Jun Kurii, and Kenzo Tonomura. "Construction of a Promoter-cloning Vector inZymomonas mobilis." Agricultural and Biological Chemistry 50, no. 11 (November 1986): 2959–61. http://dx.doi.org/10.1080/00021369.1986.10867865.

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28

Makino, Sou-ichi, Chihiro Sasakawa, Ikuo Uchida, Nobuyuki Terakado, and Masanosuke Yoshikawa. "Transformation of a cloning vector, pUB110, intoBacillus anthracis." FEMS Microbiology Letters 44, no. 1 (September 1987): 45–48. http://dx.doi.org/10.1111/j.1574-6968.1987.tb02239.x.

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29

De Rossi, E., P. Brigidi, N. E. Welker, G. Riccardi, and D. Matteuzzi. "New shuttle vector for cloning in Bacillus stearothermophilus." Research in Microbiology 145, no. 8 (January 1994): 579–83. http://dx.doi.org/10.1016/0923-2508(94)90074-4.

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30

Li, Xiaohua, Xiufen Zhou, and Zixin Deng. "Vector Systems Allowing Efficient Autonomous or Integrative Gene Cloning in Micromonospora sp. Strain 40027." Applied and Environmental Microbiology 69, no. 6 (June 2003): 3144–51. http://dx.doi.org/10.1128/aem.69.6.3144-3151.2003.

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ABSTRACT Vector systems allowing autonomous or site-specific integrative gene cloning were developed for Micromonospora sp. strain 40027, a producer of the antibiotic fortimicin A. The autonomous system depends on the discovery of a low-copy-number, self-transmissible covalently closed circular plasmid, pJTU112 (ca. 14.1 kb), which was shown to be present in the progenitor strain in both integrated and autonomous states. The copy numbers of both wild-type pJTU112 and three derivatives of it can be amplified at least sixfold by addition of streptomycin to the culture medium. The integrative system was developed by the use of a pBR322-derived Escherichia coli plasmid vector, pSET152, mediated by the attP site of the Streptomyces phage ΦC31. Both vectors can be transferred by conjugation from E. coli into Micromonospora sp. strain 40027. The heterologous cloning and expression of the dnd gene cluster originating from Streptomyces lividans 1326 into Micromonospora sp. strain 40027 demonstrated the use of the two systems.

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31

Jeong, Jae-Yeon, Hyung-Soon Yim, Ji-Young Ryu, Hyun Sook Lee, Jung-Hyun Lee, Dong-Seung Seen, and Sung Gyun Kang. "One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies." Applied and Environmental Microbiology 78, no. 15 (May 18, 2012): 5440–43. http://dx.doi.org/10.1128/aem.00844-12.

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ABSTRACTWe developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

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32

Domínguez-Martínez, Verónica, Gabriel Guarneros-Peña, Magdalena Segura-Nieto, and Everardo Curiel-Quesada. "Construction of a multiframe vector to express coding sequences inEscherichia coli." Canadian Journal of Microbiology 47, no. 1 (January 1, 2001): 72–76. http://dx.doi.org/10.1139/w00-110.

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Cloning of foreign DNA fragments for coding sequence analysis in Escherichia coli usually involves sets of three vectors. To simplify this, we constructed an expression vector named pMFV7 containing three ATG codons in different frames downstream of a Shine-Dalgarno sequence, assuming that the ribosome can use any of the three start codons in an alternative manner. Translation beginning at either of the start codons would drive the expression of any coding fragment cloned downstream. To test the feasibility of this proposal, we cloned DNA fragments of the lacZ gene in each of the possible reading frames downstream from pMFV7 start codons. Sequence analysis of the N-terminus regions around the fusion sites indicates that ribosomes indeed initiate translation at each of the three initiation codons. In one case, levels of β-galactosidase activity depended largely on the N-terminus of the translation products. We conclude that pMFV7 may be useful for expressing coding sequences regardless of their reading frame.Key words: translation initiation, in-frame gene cloning, expression vectors.

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33

Gonçalves, Manuel A. F. V., Ietje van der Velde, Josephine M. Janssen, Bram T. H. Maassen, Evert H. Heemskerk, Dirk-Jan E. Opstelten, Shoshan Knaän-Shanzer, Dinko Valerio, and Antoine A. F. de Vries. "Efficient Generation and Amplification of High-Capacity Adeno-Associated Virus/Adenovirus Hybrid Vectors." Journal of Virology 76, no. 21 (November 1, 2002): 10734–44. http://dx.doi.org/10.1128/jvi.76.21.10734-10744.2002.

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ABSTRACT Effective gene therapy is dependent on safe gene delivery vehicles that can achieve efficient transduction and sustained transgene expression. We are developing a hybrid viral vector system that combines in a single particle the large cloning capacity and efficient cell cycle-independent nuclear gene delivery of adenovirus (Ad) vectors with the long-term transgene expression and lack of viral genes of adeno-associated virus (AAV) vectors. The strategy being pursued relies on coupling the AAV DNA replication mechanism to the Ad encapsidation process through packaging of AAV-dependent replicative intermediates provided with Ad packaging elements into Ad capsids. The generation of these high-capacity AAV/Ad hybrid vectors takes place in Ad early region 1 (E1)-expressing cells and requires an Ad vector with E1 deleted to complement in trans both AAV helper functions and Ad structural proteins. The dependence on a replicating helper Ad vector leads to the contamination of AAV/Ad hybrid vector preparations with a large excess of helper Ad particles. This renders the further propagation and ultimate use of these gene delivery vehicles very difficult. Here, we show that Cre/loxP-mediated genetic selection against the packaging of helper Ad DNA can reduce helper Ad vector contamination by 99.98% without compromising hybrid vector rescue. This allowed amplification of high-capacity AAV/Ad hybrid vectors to high titers in a single round of propagation.

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34

Lefebre, Matthew D., and Miguel A. Valvano. "Construction and Evaluation of Plasmid Vectors Optimized for Constitutive and Regulated Gene Expression in Burkholderia cepacia Complex Isolates." Applied and Environmental Microbiology 68, no. 12 (December 2002): 5956–64. http://dx.doi.org/10.1128/aem.68.12.5956-5964.2002.

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ABSTRACT Genetic studies with Burkholderia cepacia complex isolates are hampered by the limited availability of cloning vectors and by the inherent resistance of these isolates to the most common antibiotics used for genetic selection. Also, some of the promoters widely employed for gene expression in Escherichia coli are inefficient in B. cepacia. In this study, we have utilized the backbone of the vector pME6000, a derivative of the pBBR1 plasmid that was originally isolated from Bordetella bronchiseptica, to construct a set of vectors useful for gene expression in B. cepacia. These vectors contain either the constitutive promoter of the S7 ribosomal protein gene from Burkholderia sp. strain LB400 or the arabinose-inducible P BAD promoter from E. coli. Promoter sequences were placed immediately upstream of multiple cloning sites in combination with the minimal sequence of pME6000 required for plasmid maintenance and mobilization. The functionality of both vectors was assessed by cloning the enhanced green fluorescent protein gene (e-gfp) and determining the levels of enhanced green fluorescent protein expression and fluorescence emission for a variety of clinical and environmental isolates of the B. cepacia complex. We also demonstrate that B. cepacia carrying these constructs can readily be detected intracellularly by fluorescence microscopy following the infection of Acanthamoeba polyphaga.

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35

Borovkov, Alex Y., and Mark I. Rivkin. "XcmI-Containing Vector for Direct Cloning of PCR Products." BioTechniques 22, no. 5 (May 1997): 812–14. http://dx.doi.org/10.2144/97225bm04.

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36

Meanger, Jayesh, Irene Peroulis, and John Mills. "Modified Semliki Forest Virus Expression Vector that Facilitates Cloning." BioTechniques 23, no. 3 (September 1997): 432–36. http://dx.doi.org/10.2144/97233bm18.

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37

Pitulle, Christian, and Norman R. Pace. "T-Cloning Vector for Plasmid-Based 16S rDNA Analysis." BioTechniques 26, no. 2 (February 1999): 222–24. http://dx.doi.org/10.2144/99262bm08.

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38

Pang, Chang-Hong, Masako Shiiyama, Haruo Ikeda, Haruo Tanaka, and Satoshi Omura. "Cosmid Vector for Cloning and Analysis of Streptomyces DNA." Actinomycetologica 8, no. 1 (1994): 21–25. http://dx.doi.org/10.3209/saj.8_21.

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39

YANASE, Hideshi, Jun KURII, and Kenzo TONOMURA. "Construction of a promoter-cloning vector in Zymomonas mobilis." Agricultural and Biological Chemistry 50, no. 11 (1986): 2959–61. http://dx.doi.org/10.1271/bbb1961.50.2959.

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40

Jones, Michael D., Nigel J. Brand, and Alan R. Fersht. "Single-stranded M13 DNA: use as a cloning vector." Nucleic Acids Research 14, no. 24 (1986): 10116. http://dx.doi.org/10.1093/nar/14.24.10116.

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41

Wu, Yaling, Shui-Pang Tam, and Peter L. Davies. "A modified CAT expression vector with convenient cloning sites." Nucleic Acids Research 18, no. 7 (1990): 1919. http://dx.doi.org/10.1093/nar/18.7.1919.

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42

de Grado, Myriam, Pablo Castán, and José Berenguer. "A High-Transformation-Efficiency Cloning Vector for Thermus thermophilus." Plasmid 42, no. 3 (November 1999): 241–45. http://dx.doi.org/10.1006/plas.1999.1427.

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43

Ho-Dong, Lim, Cheong Dae-Eun, and Kim Geun-Joong. "Construction of a Highly Reliable TA-Cloning Vector System." Journal of Biotechnology 150 (November 2010): 518. http://dx.doi.org/10.1016/j.jbiotec.2010.09.827.

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44

Grimaldi, G., G. Manfioletti, and C. Schneider. "A λ vector for directional cDNA cloning andin vitrotranscription." Nucleic Acids Research 15, no. 22 (1987): 9608. http://dx.doi.org/10.1093/nar/15.22.9608.

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Alexander, Danny C., Thomas D. McKnight, and Bill G. Williams. "A simplified and efficient vector-primer cDNA cloning system." Gene 37, no. 1-3 (1985): 279. http://dx.doi.org/10.1016/0378-1119(85)90285-9.

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Lacks, Sanford A., and Bill Greenberg. "Sequential cloning by a vector walking along the chromosome." Gene 104, no. 1 (July 1991): 11–17. http://dx.doi.org/10.1016/0378-1119(91)90458-n.

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Tu, Jun, Ping Zhu, Ke-di Cheng, and Jiangguo Liu. "Cloning and Expression of Taxus Acyltransferase cDNA." Zeitschrift für Naturforschung C 59, no. 9-10 (October 1, 2004): 755–61. http://dx.doi.org/10.1515/znc-2004-9-1022.

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Abstract A new full-length acyltransferase cDNA was obtained from Taxus chinensis by homologybased cloning strategy. The cDNA has an open-reading frame of 1,275 nucleotides, which encodes 425 amino acids with a calculated molecular weight of 47,241 Da and an estimated pI value of 5.93. The deduced amino acid sequence resembles the sequences of other cloned acyltransferases (56-61% identity; 71-75% similarity) involved directly in taxol biosynthetic pathways. This cDNA was expressed in Escherichia coli using the expression vector pET- 32a(+). The expression band corresponds to the calculated mass plus the N-terminal fusion protein derived from the vector.

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Sørensen, Kim I., Rasmus Larsen, Annette Kibenich, Mette P. Junge, and Eric Johansen. "A Food-Grade Cloning System for Industrial Strains of Lactococcus lactis." Applied and Environmental Microbiology 66, no. 4 (April 1, 2000): 1253–58. http://dx.doi.org/10.1128/aem.66.4.1253-1258.2000.

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ABSTRACT We have previously reported the construction of a food-grade cloning vector for Lactococcus using the ochre suppressor,supB, as the selective marker. This vector, pFG1, causes only a slight growth inhibition in the laboratory strain MG1363 but is unstable in the industrial strains tested. As supBsuppresses both amber and ochre stop codons, which are present in 82% of all known lactococcal genes, this undesirable finding may result from the accumulation of elongated mistranslated polypeptides. Here, we report the development of a new food-grade cloning vector, pFG200, which is suitable for overexpressing a variety of genes in industrial strains of Lactococcus lactis. The vector uses an amber suppressor, supD, as selectable marker and consists entirely of Lactococcus DNA, with the exception of a small polylinker region. Using suppressible pyrimidine auxotrophs, selection and maintenance are efficient in any pyrimidine-free medium including milk. Importantly, the presence of this vector in a variety of industrial strains has no significant effect on the growth rate or the rate of acidification in milk, making this an ideal system for food-grade modification of industrially relevant L. lactisstrains. The usefulness of this system is demonstrated by overexpressing the pepN gene in a number of industrial backgrounds.

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Alajez, Nehad M., Saman Eghtesad, and Olivera J. Finn. "Cloning and Expression of Human Membrane-Bound and Soluble Engineered T Cell Receptors for Immunotherapy." Journal of Biomedicine and Biotechnology 2006 (2006): 1–9. http://dx.doi.org/10.1155/jbb/2006/68091.

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We report here the design and construction of several gene vectors for expression in mammalian cells of membrane-bound and soluble human T cell receptors (TR). We designed a vector (TR-ALPHA-IRES-TR-BETA pEF4) that encodes high-level expression of the full-length TR on the surface of T cells. Furthermore, we engineered TR that does not require the presence of endogenous CD3 molecules for surface expression and thus expression is not limited to T cells. We also constructed a vector encoding a single-chain TR (scTR) as a fusion protein of V-ALPHA-V-BETA-C-BETA with CD3Z. Since it is encoded and expressed as a single molecule, this scTR is well suited for gene therapy. Lastly, we successfully used a mammalian expression vector for generation of soluble human TR. The approaches we used here for manipulation of a human tumor-specific TR can be useful for other investigators interested in TR-based immunotherapy.

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Lee, John S., Angela G. Hadjipanayis, and Susan L. Welkos. "Venezuelan Equine Encephalitis Virus-Vectored Vaccines Protect Mice against Anthrax Spore Challenge." Infection and Immunity 71, no. 3 (March 2003): 1491–96. http://dx.doi.org/10.1128/iai.71.3.1491-1496.2003.

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ABSTRACT Anthrax, a disease usually associated with herbivores, is caused by the bacterium Bacillus anthracis. The current vaccine licensed for human use requires a six-dose primary series and yearly boosters and causes reactogenicity in up to 30% of vaccine recipients. A minimally reactogenic vaccine requiring fewer inoculations is warranted. Venezuelan equine encephalitis (VEE) virus has been configured for use as a vaccine vector for a wide variety of immunogens. The VEE vaccine vector is composed of a self-replicating RNA (replicon) containing all of the VEE virus nonstructural genes and a multiple-cloning site in place of the VEE structural genes. Four different anthrax vaccines were constructed by cloning the protective antigen (PA) gene from B. anthracis into the VEE vaccine vector. The anthrax vaccines were produced by assembling the vectors into propagation-deficient VEE replicon particles in vitro. A/J mice inoculated subcutaneously with three doses of the mature 83-kDa PA vaccine were completely protected from challenge with the Sterne strain of B. anthracis. Similar results were obtained with vaccines composed of the PA gene fused to either the B. anthracis secretory sequence or to a tissue plasminogen activator secretory sequence in three additional mouse strains. Mice were unprotected from challenge after inoculation with the carboxy-terminal 63-kDa PA vaccine. These results suggest that these VEE-vectored vaccines may be suitable as candidate vaccines against anthrax.

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