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Dissertations / Theses on the topic 'Cloning'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Lishchyna, V., Нiна Володимирiвна Мальована, Нина Владимировна Малеванная, and Nina Volodymyrivna Malovana. "Therapeutic cloning." Thesis, Sumy State University, 2020. https://essuir.sumdu.edu.ua/handle/123456789/78011.

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The review is devoted to the current biomedical direction in cell-replacement therapy - therapeutic cloning, which is the most universal approach for obtaining patient-specific embryonic stem cell lines (hESC) with tremendous potential in maintaining and restoring human health. Now the main sources of stem cells directly for biomedical work are stem cells from umbilical cord blood and adult stem cells. Both sources have serious limitations: umbilical cord blood stem cells are autogenous only to the newly born, and receiving stem cells from the patient himself is unsafe for him. In addition, i

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2

Koehler, Daniela. "Cloning in Cattle." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-99159.

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3

Riley, D. J. S. "More than semantic differences obstructing a cloning ban at the United Nations /." Theological Research Exchange Network (TREN) Access this title online, 2004. http://www.tren.com.

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4

Källstrand, Björn. "Optimal, universal quantum cloning." Thesis, Karlstads universitet, Institutionen för ingenjörsvetenskap och fysik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-28341.

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The no-cloning theorem is one of the fundamental concepts of quantum information theory. It tells us that no general quantum state can be perfectly replicated. In this thesis we introduce the notion of imperfect cloning, and define the properties of the universal cloning machine. Furthermore, we construct an ansatz of how our universal cloning machine should perform as to produce two imperfect clones from one input qubit. We find an optimal fidelity of 5/6 for our universal cloning machine. We then reevaluate our ansatz and construct a class of unitary transformations such that an optimal fide

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5

Rajagopal, Manoj Kumar. "Cloning with gesture expressivity." Phd thesis, Institut National des Télécommunications, 2012. http://tel.archives-ouvertes.fr/tel-00719301.

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Virtual environments allow human beings to be represented by virtual humans or avatars. Users can share a sense of virtual presence is the avatar looks like the real human it represents. This classically involves turning the avatar into a clone with the real human's appearance and voice. However, the possibility of cloning the gesture expressivity of a real person has received little attention so far. Gesture expressivity combines the style and mood of a person. Expressivity parameters have been defined in earlier works for animating embodied conversational agents.In this work, we focus on exp

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6

Fisher, Adam B. "ex vivo DNA cloning." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3962.

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Genetic engineering of microbes has developed rapidly along with our ability to synthesize DNA de novo. Yet, even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. While technological advances have resulted in powerful techniques for in vitro and in vivo assembly of DNA, each suffers inherent disadvantages. Here, an ex vivo DNA cloning suite using crude cellular lysates derived from E. coli is demonstrated to amplify and assemble DNA containing small sequence homol

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7

McCarrey, Sariah Cottrell. "Personhood and Cloning: Modern Applications and Ethics of Stem Cell and Cloning Technology." BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/4170.

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Within many communities and religions, including the LDS community, there is some controversy surrounding the use of stem cells – particularly embryonic stem cells (ESC). Much of this controversy arises from confusion and misconceptions about what stem cells actually are, where they come from , and when life begins. The theology of the Church of Jesus Christ of Latter-day Saints has interesting implications for the last of these considerations, and it becomes less a question of “when does life begin” and more an exploration of “when does personhood begin” or “when does the spirit enter the bo

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8

Bates, Nancy Carol. "Characterization of cbg : a cloned gene encoding an extracellular [beta]-glucosidase from Cellulomonas fimi." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26163.

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A group of Escherichia coli clones harbouring recombinant pBR322 plasmid, containing Cellulomonas fimi DNA inserts, that reacted with antiserum to C.fimi culture supernatant, was screened for their ability to hydrolyze carboxymethyl cellulose (CMC) and 4-methylumbeliferyll-β-D-cellobioside (MUC). A clone, pEC62, hydrolyzed MUC but did not hydrolyze CMC. The recombinant enzyme encoded by pEC62 was shown to be a β-glucosidase (cellobiase). C.fimii itself was shown to encode an extracellular β-glucosidase in C.fimi. This is the first report of an extracellular β-glucosidase from a bacterium. Del

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9

Pelser, Adam C. "Made in the image of man the value of Christian theology for public moral discourse on human cloning /." Electronic thesis, 2007. http://dspace.zsr.wfu.edu/jspui/handle/10339/187.

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10

Lee, Rivera Irene. "Cloning and characterization of mTom40." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ50814.pdf.

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11

Lee, Rivera Irene. "Cloning and characterization of mTom40." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21588.

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The majority of mitochondrial proteins are synthesized on cytoplasmic polysomes and subsequently imported to a specific subcompartment within the organelle. In order to translocate cytosolic proteins into mitochondria a protein import system is located in the outer and inner mitochondrial membranes. The genetic composition of this complex has been well characterized in mitochondria of S. cerevisiae and N. crassa. However, little is known about the components of the import machinery in higher eukaryotes. Until today only homologues of Tom20 and Tom17 as well as two new proteins Metaxin and hTom

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12

Dent, C. L. "cDNA cloning and the retina." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384395.

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13

Walsh, Maura Stephanie. "Cloning fungal polyketide synthase genes." Thesis, University of Bristol, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333957.

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14

Weinstein, Earl G. 1974. "MicroRNA cloning and bioinformatic analysis." Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/8390.

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Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 2002.<br>Includes bibliographical references.<br>Part I. Two gene-regulatory noncoding RNAs (ncRNAs), let-7 RNA and lin-4 RNA, were previously discovered in the C. elegans genome. The let-7 gene is conserved across a wide range of genomes, suggesting that these ncRNAs represent a wider class of gene-regulatory RNAs. Both lin-4 and let-7 RNAs are generated from stem-loop precursor RNAs, and share a common biochemical signature, namely 5'-terminal phosphate and 3'-terminal hydroxyl groups. We refer to ncRNAs that share the

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15

Подолкова, Світлана Віталіївна, Светлана Витальевна Подолкова, Svitlana Vitaliivna Podolkova, and M. V. Ruban. "Reasons for against human cloning." Thesis, Видавництво СумДУ, 2010. http://essuir.sumdu.edu.ua/handle/123456789/18316.

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16

Gustavsson, Alma. "Cloning of an aldolase mutant." Thesis, Uppsala universitet, Institutionen för kemi - BMC, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-397493.

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17

Seto, Nina Oi Ling. "The copper-zinc superoxide dismutase gene from Drosophila melanogaster : attempts to clone the gene using two mixed sequence oligonucleotide probes." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26534.

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Superoxide dismutase is an enzyme which scavenges superoxide radicals and is thought to be a longevity determinant, as there exists a positive correlation between superoxide dismutase concentration and maximum life span potential. The cytosolic CuZn superoxide dismutase in D. melanogaster has been purified and sequenced, but the gene has not been cloned. However, when it is available the CuZn SOD gene may be reintroduced into the Drosophila genome via the P-element transformation system so its effects on the life span potential of Drosophila may be studied. This study describes attempts to clo

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18

Woodruff, Wendy Anne. "Cloning and characterization of the oprF gene for protein F from Pseudomonas aeruginosa." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29218.

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The oprF gene encoding porin protein F from Pseudomonas aeruginosa was cloned onto a cosmid vector into Escherichia coli. Protein F was expressed in large amounts in E. coli and retained its heat- and reduction-modifiable and immunological characteristics. The cloned oprF gene product was purified from E. coli and characterized with respect to pore-forming ability in black lipid bilayers. Small channels, with an average single channel conductance of approximately 0.4 nS, were observed. A similar small channel size was observed for native protein F. The oprF sequences were used as a DNA-DNA hyb

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19

Boyes, Barry Edward. "Molecular cloning of the human Substantia innominata : characterization of a brain large mRNA." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30960.

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Brain tissue samples were collected from individuals with histologically and biochemically confirmed Alzheimer's Disease (AD), as well as from a group of individuals without any signs of neurological disease (NNC). Ribonucleic acid (RNA) was extracted from these tissues, characterized by several chemical methods, and the yields were compared between AD and NNC groups. High molecular weight RNA could be effectively extracted from frozen postmortem human brain. In comparison to the NNC group, tissue RNA levels were reduced in the AD hippocampus, but not in the temporal cortex or substantia innom

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20

Wakarchuk, Warren William. "The molecular cloning and characterization of a Beta-glucosidase gene from an Agrobacterium." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27559.

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The β-glucosidase (Abg) from ATCC 21400, an Agrobacterium species, was purified to homogeneity. The protein was cleaved with cyanogen bromide and the peptides were purified by reversed phase high pressure liquid chromatography. The partial amino-acid sequences for three CNBr peptides, CNBr1, CNBr2 and CNBr3, were determined by automated Edman degradation. A sequence from CNBr2 was used to synthesize a mixture of oligonucleotides which was used as a hybridization probe to identify a recombinant DNA clone carrying the gene for β-glucosidase. A single clone was isolated which expressed an enzymat

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21

Chang, Li-Ching. "Cloning, purification and crystallization of selenophosphate synthetase cloning, purification and crystallization of ERp44 from Mus musculus /." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=980297311.

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22

Österdahl, Fredrik, and Ilias Hendo. "Themes in Totara : Creating and cloning." Thesis, Karlstads universitet, Institutionen för matematik och datavetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-47678.

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The purpose of this paper is to give the reader an insight into the graphical interface of a Learning Management system, the theme, how it functions and its importance. The main point is how the creation of new themes can be done, and two different approaches are examined and compared.The use of learning management systems have been growing rapidly over the past years. Modern learning, be it basic school, higher education or professional training, is almost always supported by some learning management system where both instructors and participators share learning material, assignments, discuss

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23

Lush, Michael John. "Molecular cloning of neuropathy target esterase." Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/29627.

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A single ingestion of certain organophosphorus esters (OPs) can cause a syndrome known as Organophosphate Induced Delayed Polyneuropathy (OPIDP), a paralysing neuropathy with degeneration of long axons, developing after a latent period of approximately one to three weeks. The primary target of these OPs has been shown to be a 155kDa neural protein designated Neuropathy Target Esterase (NTE), and the toxic effects apparently due to the covalent inhibition and subsequent secondary modification of this protein. Recently NTE has been purified to apparent homogeneity using a novel biotinylated OP a

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24

Isaac, Andrew Paul Computer Science &amp Engineering Faculty of Engineering UNSW. "Behavioural cloning robust goal directed control." Awarded By:University of New South Wales. Computer Science & Engineering, 2009. http://handle.unsw.edu.au/1959.4/43367.

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Behavioural cloning is a simple and effective technique for automatically and non-intrusively producing comprehensible and implementable models of human control skill. Behavioural cloning applies machine learning techniques to behavioural trace data, in a transparent manner, and has been very successful in a wide range of domains. The limitations of early behavioural cloning work are: that the clones lack goal-structure, are not robust to variation, are sensitive to the nature of the training data and often produce complicated models of the control skill. Recent behavioural cloning work has so

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25

Jeromin, Andreas. "Frequenin in crustaceans, cloning and immunolocalization." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ33955.pdf.

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26

Lundberg, Mathias. "Cloning and characterization of human glutaredoxins /." Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-261-2.

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27

Tsoi, Carrie. "Cloning and characterization of canine sulfotransferases /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-679-0/.

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28

Long, Graham Stanley. "Molecular cloning of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.

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29

Sakuntabhai, Anavaj. "Positional cloning of Darier's disease gene." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302392.

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30

Dunn, Alison M. "Cloning of human DNA repair genes." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301385.

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31

McNair, Alan Thomas. "Molecular cloning of Fasciola hepatica antigens." Thesis, Queen's University Belfast, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335604.

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32

Reza, Faisal 1980. "Human cloning : science, ethics, policy, society." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/29582.

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Thesis (B.S.)--Massachusetts Institute of Technology, Program in Science, Technology, and Society, 2003.<br>Includes bibliographical references (leaves 73-74).<br>The interplay of science, ethics, policy and society contribute to our understanding of and relation with human cloning. Genetic science and technology at the end of the twentieth century has permitted successful cloning of mammals and other animals. Such advancement has raised key ethical issues regarding the prospect of cloning human beings. Evaluation of these issues has led to policies aimed at regulating this novel technology. I

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33

Martínez-García, Juan Carlos. "Expression cloning of insecticidal toxin receptors." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621840.

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34

Hinson, Gary. "Cloning of an Escherichia coli adhesin." Thesis, University of Leicester, 1987. http://hdl.handle.net/2381/34424.

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Pathogenic bacteria colonise their host animals by means of a complex set of interactions. The host defensive mechanisms attack foreign microorganisms and attempt to rid the animal of the invaders, while the bacteria express a variety of functions to ensure their survival under adverse conditions, some of which damage the host and cause the clinical symptoms of disease. Adhesins are the bacterial structures which mediate adherence to specific host tissues and therefore permit the colonisation of areas from which the bacteria would normally be removed. T4. I have genetically cloned and analysed

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35

Yamada, Yuichiro. "Cloning of a somatostatin receptor family." Kyoto University, 1994. http://hdl.handle.net/2433/160717.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである<br>Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(医学)<br>甲第5815号<br>医博第1594号<br>新制||医||592(附属図書館)<br>UT51-94-X197<br>京都大学大学院医学研究科内科系専攻<br>(主査)教授中尾一和, 教授成宮周, 教授森徹<br>学位規則第4条第1項該当

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36

Brihn, Lesil E. "POSITIONAL CLONING OF THE DISORGANIZATION MUTATION." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1189146887.

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37

Choi, Wai To. "Molecular cloning of ribosome-inactivating proteins." HKBU Institutional Repository, 1996. http://repository.hkbu.edu.hk/etd_ra/63.

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38

Pratap, Amrit Abu-Mostafa Yaser S. Abu-Mostafa Yaser S. "Adaptive learning algorithms and data cloning /." Diss., Pasadena, Calif. : Caltech, 2008. http://resolver.caltech.edu/CaltechETD:etd-05292008-231048.

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39

Moser, Bernhard. "Molecular cloning, characterization and expression of the endoglucanase C gene of Cellulomonas fimi and properties of the native and recombinant gene products." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29036.

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In addition to substrate-associated cellulases, Cellulomonas fimi secretes endoglucanases ( endo-1, 4-β-D-glucan glucanohydrolases, EC 3.2.1.4. ) which are recovered from the cellulose-free culture supernatant of cells grown on microcrystalline cellulose. Two such enzymes, C3.1 and C3.2 with Mrs of 130'000 and 120'000, respectively, were purified to homogeneity. The two endoglucanases were shown to share the same N-terminal amino acid sequence and to hydrolyze carboxymethylcellulose ( CMC ) with similar efficiencies ( 236u/mg protein for C3.1 and 367u/mg protein for C3.2 ). The recombinant la

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40

Doucette, Stephanie A. "Cloning of Bovine Placental Lactogen and Production in Vitro." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/DoucetteSA2003.pdf.

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41

Tsie, Marlene S. "Cloning and Immunolocalization of G Proteins in the Spionid Polychaete Dipolydora quadrilobata." Fogler Library, University of Maine, 2006. http://www.library.umaine.edu/theses/pdf/TsieMS2006.pdf.

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42

Zhang, Ling 1962. "Molecular cloning and characterization of the chicken ornithine decarboxylase gene." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22831.

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Ornithine decarboxylase (ODC) is the rate determining enzyme in the biosynthesis of polyamines which are essential for cell growth. In chickens, significantly higher bioactivity is reported in broiler than in egg layer strains of chickens (Bulfield et al., 1988). To characterize the genetic differences in growth rates and ODC levels in chickens, an ODC cDNA and genomic gene were cloned and sequenced. Sequencing of ODC cDNA revealed that this clone (pODZ3: 2,052 bp) was not a full length of ODC cDNA and contained 2 putative introns. The open reading frame (introns deleted) coded for a protein o

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43

Suen, Ki-Ling. "Cloning of protein kinase genes from a carrot cDNA library." Diss., Georgia Institute of Technology, 1991. http://hdl.handle.net/1853/25431.

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44

Cammas, Florence Marie. "Characterisation of the mouse ACTH receptor promoter." Thesis, Queen Mary, University of London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244208.

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45

Barton, Jenny Loretta. "IL-1L1 : a novel gene in the human interleukin-1 cluster." Thesis, University of Sheffield, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366223.

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46

Wain, Hester Mary. "Targeted mapping of the chicken genome." Thesis, University of Hertfordshire, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338594.

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47

Godwin, Rosamond Mary. "Cloning and characterisation of genes encoding phosphate and sulphate transporters from rice /." St. Lucia, Qld, 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16400.pdf.

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48

Freeman, John Douglas. "The cloning of polyhomeotic, a complex Drosophila locus required for segment determination and cuticular differentiation." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26256.

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The polyhomeotic (ph) locus of Drosophila melanogaster has been characterized genetically. Early studies showed that ph is a member of the Polycomb (Pc) group. These genes have similar phenotypes and are required for normal segment determination. Recent analyses of amorphic ph mutations show that the ph locus is complex, has a strong maternal effect and plays a role in cuticular development. To test the function of ph at the molecular level, the cloning of the ph locus was undertaken. One strain had been shown to contain a P element insertion near ph. A genomic library was prepared from this s

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49

Mirhosseini, Negin. "Cloning and expression of equine NF-kB2." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2838.

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50

Skeggs, Patricia Ann. "Cloning of aminoglycoside-resistance determinants in Streptomyces." Thesis, University of Leicester, 1986. http://hdl.handle.net/2381/35152.

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