Relevant bibliographies by topics / Clonky

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Clonky'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "Clonky"

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de Boer, I. J. M., T. H. E. Meuwissen, and J. A. M. van Arendonk. "Combining the genetic and clonal responses in a closed dairy cattle nucleus scheme." Animal Science 59, no. 3 (December 1994): 345–58. http://dx.doi.org/10.1017/s000335610000787x.

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AbstractDesigns testing clones in a closed nucleus, in which 1024 cows are tested each year, were compared for their additive genetic response to selection (genetic response) and their genetic superiority of female genotype(s) selected for commercial cloning (clonal response), using stochastic simulation. Clones were tested at the expense of dam or sire families, matings per dam (sire), or full-sibs per family. The reference design maximized the genetic response corrected for inbreeding in the absence of cloning. The trait considered was overall economic merit for milk production, which was simulated assuming an approximate infinitesimal model with both additive and dominant gene action. Bulls and cows eligible for breeding were selected on their animal model estimated additive genetic effect at either 15 or 27 months of age. Female genotypes eligible for commercial cloning were selected on their estimated total genetic effect at 27 months of age. All (fe)male full-sibs were available for selection. With only additive gene action, testing clones at the expense of sire families, matings per dam or full-sibs per family reduced genetic response, while it increased clonal response and inbreeding. Testing clones at the expense of dam families, however, added to both the genetic and clonal response without increasing inbreeding. When eight clones were tested at the expense of dam families, the genetic response and the final genetic level of commercially available cloned embryos were maximal. Accuracy of clonal selection equalled 0·83. With dominant gene action, however, testing two clones at the expense of dam families maximized the final genetic level of cloned embryos, irrespective of the level of inbreeding depression (accuracy of 0·72). Reliable commercial clone lines can be produced now and in future generations by testing clones at the expense of dam families.
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Gascan, H., J. F. Gauchat, M. G. Roncarolo, H. Yssel, H. Spits, and J. E. de Vries. "Human B cell clones can be induced to proliferate and to switch to IgE and IgG4 synthesis by interleukin 4 and a signal provided by activated CD4+ T cell clones." Journal of Experimental Medicine 173, no. 3 (March 1, 1991): 747–50. http://dx.doi.org/10.1084/jem.173.3.747.

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In the present study, it is demonstrated that cloned surface IgM-positive human B cells can be induced to proliferate and to switch with high frequencies to IgG4 and IgE production after a contact-mediated signal provided by T cell clones and interleukin 4 (IL-4). This T cell signal is antigen nonspecific and is provided by activated CD4+ cells, whereas activated CD8+ or resting CD4+ T cell clones are ineffective. 15-35% of the B cell clones cultured with cloned CD4+ T cells and IL-4 produced antibodies; 35-45% of those wells in which antibodies were produced contained IgE and IgG4. In addition to B cell clones that produced IgG4 or IgE only, B cell clones producing multiple isotypes were observed. Simultaneous production of IgG4 and IgE, IgM, IgE, and IgM, or IgG4 and IgE was detected, suggesting that during clonal expansion switching might occur in successive steps from IgM to IgG4 and IgE. In addition, production of only IgM, IgG4, and IgE during clonal expansion indicates that this isotype switching is directed by the way a B cell is stimulated and that it is not a stochastic process.
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Shi, C., and J. W. Mellors. "A recombinant retroviral system for rapid in vivo analysis of human immunodeficiency virus type 1 susceptibility to reverse transcriptase inhibitors." Antimicrobial Agents and Chemotherapy 41, no. 12 (December 1997): 2781–85. http://dx.doi.org/10.1128/aac.41.12.2781.

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We have developed a new recombinant retroviral system in which a library of infectious molecular clones of human immunodeficiency virus type 1 (HIV-1) is constructed with reverse transcriptase (RT) genes derived from viral RNA sequences in plasma. HIV-1 RT is amplified from plasma HIV-1 RNA by nested RT-PCR and cloned into a RT-defective HIV-1 proviral vector (xxLAI-np), generating 10(3) to 10(4) recombinant proviral clones from each reaction. The bulk cloning products or individual molecular clones are transfected into MT-2 cells to generate infectious virus. The resultant viruses are assayed for drug susceptibility in CD4+ cell lines to determine either the dominant phenotype of the recombinant virus mixture or the phenotypes of the individual viral clones. DNA sequencing of the cloned RT genes can identify mutations associated with phenotypic resistance of clonal mixtures or individual clones. This method can be used to rapidly detect the in vivo emergence of HIV-1 quasispecies resistant to RT inhibitors.
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Morzaria, S. P., T. T. Dolan, R. A. I. Norval, R. P. Bishop, and P. R. Spooner. "Generation and characterization of clonedTheileria parvaparasites." Parasitology 111, no. 1 (July 1995): 39–49. http://dx.doi.org/10.1017/s0031182000064581.

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SUMMARYA 3-step procedure for cloningTheileria parvaparasites was developed. The first step involved thein vitroinfection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then selected for cloning as these contained schizont-infected cells, each of which was derived from infection with a single sporozoite. In the second step, these cell lines were cloned by limiting dilution. As sporozoites infect lymphocytes and transform to induce clonal multiplication, this step produced infected cell lines containing both cloned parasites and cloned lymphocytes. In the third step, the cloned cell lines were used to infect cattle and isolation of the parasite in ticks was made during piroplasm parasitaemia. Finally, sporozoites were harvested from infected ticks and used for further characterization. Sporozoites derived from cloned cell lines ofT. parvaMuguga, Marikebuni, Boleni, Uganda and buffalo-derived 7014 were characterized using monoclonal antibody profiles, DNA restriction fragment length polymorphism detected using repetitive and telomeric probes,in vivoinfectivity and, in one case, cross-immunity studies. Additionally, several distinct schizont-infected lymphocyte clones were isolated from the Muguga, Mariakani and buffalo-derived 7014 stocks. The combined results of the characterization revealed that the cloning procedure selected clones ofT. parvafrom the parental stocks which were known to contain a mixture of genetically different parasite populations. The cloning method and the clones generated will be of value in studies of the biology of the parasite and in elucidating the strain specificity of immune responses in cattle.
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Dummer, R., PW Heald, FO Nestle, E. Ludwig, E. Laine, S. Hemmi, and G. Burg. "Sezary syndrome T-cell clones display T-helper 2 cytokines and express the accessory factor-1 (interferon-gamma receptor beta-chain)." Blood 88, no. 4 (August 15, 1996): 1383–89. http://dx.doi.org/10.1182/blood.v88.4.1383.bloodjournal8841383.

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Sezary syndrome (SS) is a leukemic variant of low-grade cutaneous T- cell lymphomas (CTCLs). The clonal T cells in this lymphoproliferative disorder are poorly characterized. Using antibodies against the variable region of the T-cell receptor (TCR V alpha/beta), we identified four predominant T-cell clones (two V beta 8+ clones, one V beta 5.1+, and one V alpha 2(a)+) in peripheral blood mononuclear cells (PBMC) of SS patients. Their phenotype was CD3+, CD4+, CD5+, CD45RO+. Clonal T cells were purified, and cytokine transcription and secretion was analyzed by reverse transcriptase-polymerase chain reaction (RT- PCR) followed by hybridization with biotinylated probes and enzyme- linked immunosorbent assays (ELISAs). The interleukin-10 (IL-10) PCR product was cloned and sequenced and found to be identical to the published cDNA sequence. The presence of accessory factor-1 (AF-1, or interferon-gamma [IFN-gamma] receptor beta-chain) encoding mRNA was assessed by RT-PCR and immunostaining using serum of rabbits immunized with the extracellular domain of a recombinant human AF-1 protein followed by APAAP staining. Clonal T cells transcribe and secrete mainly T-helper 2 cytokines (IL-10, -5, and -13). mRNA from purified SS clones but not mRNA from SS total PBMC was positive for AF-1 in an agarose gel and/or after hybridization. AF-1 transcription was associated with membrane-bound immunoreactivity for AF-1 in SS clones. SS-derived T-cell clones display T-helper 2 cytokines. This weakens cell-mediated immunosurveillance, and explains the clinical and immunologic abnormalities in SS patients. The T-helper 2 cytokine spectrum of all clones investigated is associated with overexpression of AF-1. This suggests that AF-1 is a potential marker for these clones (and eventually other T-helper 2 lymphocytes) and might represent a target for treatment of the disease.
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PANG, JOE THAU-YIN, and G. LOCKWOOD. "A RE-INTERPRETATION OF HYBRID VIGOUR IN COCOA." Experimental Agriculture 44, no. 3 (July 2008): 329–38. http://dx.doi.org/10.1017/s0014479708006431.

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SUMMARYFor 50 years, ‘hybrid vigour’ in crosses involving genotypes collected from the centre of diversity of the crop in the Peruvian Amazon has been the guiding principle of cocoa breeding. In the work described here, six Amazonian genotypes and one Trinitario clone were used to produce four bi-parental crosses. Twenty-two plants of each cross were propagated from plagiotropic buds as ‘cloned seedlings’ and evaluated for growth, yield and yield efficiency (the ratio of yield to continuing vegetative growth) in replicated trials in comparison with the parental clones and the reproduced seedling family. The reproduced seedling families grew faster than and outyielded all the clones in all four crosses, although they showed no gain in yield efficiency. The vigour and yield of the ‘cloned seedlings’ of the three inter-Amazon crosses exceeded mid-parent value (MPV), but not the single Trinitario cross. The pattern of departures from MPVs was inconsistent with current understanding of the genetic distance between the parents. The superiority of the seedlings over the ‘cloned seedlings’ is interpreted as an epigenetic effect. The results suggest that simple recurrent selection is an appropriate breeding strategy in cocoa, with different optimum planting densities for the seedling and clonal phases. It is concluded that if the concept of very large ‘hybrid vigour’ did not already exist in cocoa, it would not be adopted on the evidence of this work, the first in which clones were compared to their progeny grown as clones.
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Pohl, J., N. Goldfinger, A. Radler-Pohl, V. Rotter, and V. Schirrmacher. "p53 increases experimental metastatic capacity of murine carcinoma cells." Molecular and Cellular Biology 8, no. 5 (May 1988): 2078–81. http://dx.doi.org/10.1128/mcb.8.5.2078.

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Transfection of a cloned p53 gene into a murine bladder carcinoma cell with a low metastatic capacity led to elevated levels of p53 protein in clonal transfectants. After intravenous inoculation into syngeneic mice, p53-transfected clones showed significantly increased metastatic potential in comparison with control transfectants. The observed change did not seem to be due to a change in growth potential per se since the cell lines showed similar growth properties in vitro.
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Pohl, J., N. Goldfinger, A. Radler-Pohl, V. Rotter, and V. Schirrmacher. "p53 increases experimental metastatic capacity of murine carcinoma cells." Molecular and Cellular Biology 8, no. 5 (May 1988): 2078–81. http://dx.doi.org/10.1128/mcb.8.5.2078-2081.1988.

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Transfection of a cloned p53 gene into a murine bladder carcinoma cell with a low metastatic capacity led to elevated levels of p53 protein in clonal transfectants. After intravenous inoculation into syngeneic mice, p53-transfected clones showed significantly increased metastatic potential in comparison with control transfectants. The observed change did not seem to be due to a change in growth potential per se since the cell lines showed similar growth properties in vitro.
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Yang, Min, Iuri Perisse, Zhiqiang Fan, Misha Regouski, Mirella Meyer-Ficca, and Irina A. Polejaeva. "Increased pregnancy losses following serial somatic cell nuclear transfer in goats." Reproduction, Fertility and Development 30, no. 11 (2018): 1443. http://dx.doi.org/10.1071/rd17323.

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Serial cloning by somatic cell nuclear transfer (SCNT) is a critical tool for the expansion of precious transgenic lines or resetting the lifespan of primary transgenic cells for multiple genetic modifications. We successfully produced second-generation cloned goats using donor neonatal fibroblasts from first-generation clones. However, our attempts to produce any third-generation clones failed. SCNT efficiency decreased progressively with the clonal generations. The rate of pregnancy loss was significantly greater in recloning groups (P < 0.05). While no pregnancy loss was observed during the first round of SCNT, 14 out of 21 pregnancies aborted in the second round of SCNT and all pregnancies aborted in the third round of SCNT. In this retrospective study, we also investigated the expression of 21 developmentally important genes in muscle tissue of cloned (G1) and recloned (G2) offspring. The expression of most of these genes in live clones was found to be largely comparable to naturally reproduced control goats, but fibroblast growth factor 10 (FGF10), methyl CpG binding protein 2 (MECP2) and growth factor receptor bound protein 10 (GRB10) were differentially expressed (P < 0.05) in G2 goats compared with G1 and controls. To study the effects of serial cloning on DNA methylation, the methylation pattern of differentially methylated regions in imprinted genes H19 and insulin like growth factor 2 receptor (IGF2R) were also analysed. Aberrant H19 DNA methylation patterns were detected in G1 and G2 clones.
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Kochenderfer, James N., Sumiko Kobayashi, Eric D. Wieder, Chunliu Su, and Jeffrey J. Molldrem. "Loss of T-lymphocyte clonal dominance in patients with myelodysplastic syndrome responsive to immunosuppression." Blood 100, no. 10 (November 15, 2002): 3639–45. http://dx.doi.org/10.1182/blood-2002-01-0155.

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Evidence suggests that T lymphocyte–mediated inhibition of hematopoiesis in myelodysplastic syndrome (MDS) contributes to cytopenia in some patients and can be reversed by treatment with immunosuppression. We examined the T-cell repertoires of 12 patients with MDS before and after antithymocyte globulin (ATG)–based treatment by T-cell receptor Vβ (TCR-Vβ) spectratype analysis. The average number of TCR-Vβ families with skewed spectratypes, representative of clonal or oligoclonal T-cell populations, was 7.6 in MDS patients before treatment and 3.2 in healthy controls (P = .02). Four patients who recovered effective hematopoiesis after treatment lost prominent, skewed peaks on their spectratypes, suggesting loss or diminution of overrepresented clonal T-cell populations. In contrast, patients who did not recover effective hematopoiesis showed persistently skewed repertoires 3 to 6 months after treatment. In 3 patients with skewed repertoires, cDNA from the complementarity-determining region 3 (CDR3) of 4 TCR-Vβ families was cloned and repetitively sequenced, confirming clonal T-cell dominance in each family. In one nonresponder, 16 of 19 CDR3 sequences were identical, demonstrating that 9.3% of the total T-cell population was made up of a single clone. By 6 months after treatment, this clone persisted on both spectratype and DNA sequence complementarity and when analyzed by flow cytometry was shown to be CD8+/CD45RA+/HLA-DR−. T-cell clones were not anergic because they could be expanded 4-fold in vitro. Our results demonstrate that predominant clonal T cells that appear to be antigen-driven persist in patients with MDS unresponsive to immunosuppression, but predominant clones regress in responders to immunosuppression.
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Dissertations / Theses on the topic "Clonky"

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Hubáček, Jiří. "Srovnání podmínek proudění plynu v detektoru při užití clonky a síťky." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2010. http://www.nusl.cz/ntk/nusl-218759.

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The first part of the thesis deals with electron microscopy and physical model fluid flow. In the next part, a three-dimensional model is created in programme SolidWorks. It is the model of scintilating detector with aperture or screen with three hundred little apertures. These models are submitted to analysis focusing on investigation of the gas flow at different pressure in chamber of specimen ESEM using Cosmos FloWorks system. The results of the analysis of both apertures variants are compared with the request to minimize the pressure in the trajectory of the secondary electrons as well as in the chamber of specimen.
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Novotný, Marek. "Vyhodnocení vlivu tvaru otvorů clonek na výsledný tlak na dráze sekundárních elektronů v detektoru pomocí systému CAE." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-219193.

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This diploma thesis deals with electron microscopy. Examined equipment is environmental scanning electron microscope (ESEM), namely scintillation a detector of the microscope. There is solved the influence of the profile of holes in diaphragms to the resulting pressure and gas flow on the path of secondary electrons at the detector. Introductory part of thesis informs about microscopy in general, with concentration on electron microscopy; especially on scanning microscope, because research is taken just on the environmental scanning electron microscope. Another part informs about both general principles of dynamics of gases and with finite volume method. Another part deals with concrete used software and with setting of individual parameters for calculation. At the beginning of calculation are used five basic profiles of holes in diaphragms for pressure 1000 Pa in the chamber of the sample. For modelling individual shapes is used 3D parametric modeller SolidWorks. Analysis of circulation of secondary electrons through detector is made by using Cosmos FloWorks module. The most suitable type of diaphragms is chosen from measured models. Another part of diploma thesis deals with measuring of chosen types of diaphragms for more pressures in the chamber of the sample; the pressure is 200, 400, 600, 800 and 1000 Pa. The outcomes of this research are both models of pressure and speed of circulation inside the detector and graphically processed values by using different diaphragms, respectively one type of a diaphragm in different pressures. Production drawings of each diaphragm, together with calculated models, are enclosed.
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Koyo, Jean-Prosper. "Bouturage et variabilité morphogénétique de clones de Terminalia superba Engler et Diels ou Limba du Sud-Congo." Paris 11, 1985. http://www.theses.fr/1985PA112011.

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Les études d’amélioration génétique du Terminalia superba par voie asexuée ont été entreprises au CONGO, dans le cadre d’un projet de reboisement industriel de cette espèce. Une centaine de Limba d’élite sélectionnés dans les forêts du sud du Congo, ont été clonés et font l’objet depuis 1976 de plusieurs expérimentations et observations. Si actuellement, le bouturage est acquis et la croissance des clones encourageante, il n’en est pas de même de la réjuvénilisation qui ne semble pas encore satisfaisante, en particulier pour les caractères de branchaison. En conclusion, nous suggérons un schéma d’amélioration qui concilie la méthode asexuée et sexuée à partir des tests de provenances qui viennent d’être mis en place à NGOUA 2
Asexual propagation and morphogenetic clonal variability of Terminalia superba Engler and Diels (Limba) in South Congo. Genetic improvement of Terminalia superba by asexual propagation has been studied in Congo as part of an industrial reforestation project. One hundred elite Limba, selected in South Congo forests, were cloned and since 1976 have been studied in several trials. A technique of establishing cuttings is now well understood and the growth of clones is encouraging, but rejuvenation remains unsatisfactory, particularly as it affects branching. This thesis concludes with a suggested breeding scheme involving both sexual and asexual propagation of material being tested in recently established provenance trials
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Agrenius, Gustafsson Thomas. "Testing universal Compton clocks using clock interferometry." Thesis, KTH, Fysik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279947.

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Sharma, Rajesh kumar. "Comparison of development of radiata pine (Pinus radiata D. Don) clones in monoclonal and clonal mixture plots." Thesis, University of Canterbury. School of Forestry, 2008. http://hdl.handle.net/10092/1577.

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The development of radiata pine (Pinus radiata D. Don.) clones was compared in monoclonal and clonal mixture plots planted in an experiment established at Dalethorpe, Canterbury, New Zealand with ten radiata pine clones in September 1993. Clones were deployed in a randomised complete block plot design with three replications. Each replication contained ten treatments of monoclonal plots and one in which all the clones were intimately mixed in equal proportions. Clones significantly differed in initial morphologies, survival and stem slenderness. Sturdiness and initial heights were found to be the best predictors of initial survivals. The study revealed that mode of deployment did not affect overall productivity, but individual clones exhibited significantly different productivities between modes of deployment. All clones contributed similarly to overall productivity in the monoclonal mode of deployment, whereas the contribution of clones in the clonal mixture mode of deployment was disproportionate. A minority of the clones contributed a majority of overall productivity in the clonal mixture mode of deployment. The inclusion of competition index as an independent variable in a distance-dependent individual tree diameter increment model explained a significant amount of variability in diameter growth. The use of an inverse-squared distance to neighbouring plants in the competition index provided a slightly superior fit to the data compared to one that employed a simple inverse of distance. Addition of genotype information in the competition index further improved the fit of the model. Clones experienced different levels of competition in monoclonal and clonal mixture modes of deployment. Competition in monoclonal plots remained uniform over time, whereas some clones experienced greater competition in clonal mixture plots which led to greater variability in their tree sizes. This study indicated that single tree plot progeny test selections and early selections may miss out some good genotypes that can grow rapidly if deployed monoclonally. Stand level modelling revealed that clones differed significantly in modeled yield patterns and model asymptotes. Clones formed two distinct groups having significantly different yield models. The study also demonstrated that models developed from an initial few years’ data were biased indicators of their relative future performances. Evaluation of effectiveness of the 3-PG hybrid model using parameter values obtained from destructive sampling and species-specific values from different studies revealed that it is possible to calibrate this model for simulating the productivity of clones, and predictions from this model might inform clonal selections at different sites under differing climatic conditions. Destructive sampling at age 5 years revealed that clones significantly differed in foliage and stem biomass. The differences in productivities of clones were mainly due to differences in biomass partitioning and specific leaf areas. Clones significantly differed in dynamic wood stiffness, stem-slenderness, branch diameter, branch index and branch angle at an initial stocking of 1250 stems/ha. Mode of deployment affected stem slenderness, which is sometimes related to stiffness. Although dynamic stiffness was correlated with stem slenderness and stem slenderness exhibited a significant influence on stiffness, clones did not exhibit statistically significant differences in dynamic stiffness. Increasing initial stocking from 833 stems/ha to 2500 stems/ha resulted in a 56 % decrease in branch diameter and a 17 % increase in branch angle. Trees in the monoclonal mode of deployment exhibited greater uniformity with respect to tree size, stem-slenderness, and competition experienced by clones compared to those in the clonal mixture mode of deployment. Susceptibility of one clone to Woolly aphid suggested that greater risks were associated with large scale deployment of susceptible clones in a monoclonal mode of deployment. This study also indicated that if the plants were to be deployed in a monoclonal mode then block plot selections would have greater potential to enhance productivity.
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Frigotto, Taciana. "Seleção de espécies/procedências e propagação vegetativa de Eucalyptus spp. na região norte de Santa Catarina." Universidade do Estado de Santa Catarina, 2016. http://tede.udesc.br/handle/handle/2321.

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Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2017-12-08T11:11:14Z No. of bitstreams: 1 PGEF16MA057.pdf: 1146330 bytes, checksum: 87ce579ee0bdc4ea559098e0a886c2f6 (MD5)
Made available in DSpace on 2017-12-08T11:11:14Z (GMT). No. of bitstreams: 1 PGEF16MA057.pdf: 1146330 bytes, checksum: 87ce579ee0bdc4ea559098e0a886c2f6 (MD5) Previous issue date: 2016-02-29
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The objective was to rescue, preserve and multiply the genetic material of the best species/origins selected Eucalyptus spp., to southern Brazil and select the best clones for propagation in Commercial level. The forest Eucalyptus spp. the study is located in the city of Rio Negrinho, SC. The forest was planted in December 1999. The species of the gender Eucalyptus sp. present in the experimental area are grown from seeds, from different locations. For selection of the best sources, subjects were evaluated for survival, height, diameter at breast height, volume, and features like the trunk tortuosity and fork. For vegetative material rescue experiment was applied the techniques of annealing and semi-annealing nine potential species, where individuals were evaluated number of shoots, number of cutting, survival and rooting. The same was evaluated according to the youthfulness through different heights from the ground for E. benthamii. The potential species for quantitative and qualitative variables, as well as the ranking used to select the best species, three species that stood out were E. dunni, E. benthamii and E. dorrigoensis. Regarding the origins for E. smithii the origin Wilson Promontorj to E.benthamii Australia, as well as E. viminalis and E. deanei the origin Manville SC and E. macarthurii Pieter Maritz. Among the vegetative recovery methods tested, the girdling showed better results due to the issuance of shoots. For the survival and 12 13 rooting of seedlings it is necessary to further study since only E. deanei embedded material obtained using IBA at 3000 mg L-¹. As for the issue of shoots depending on the degree of youthfulness to E. benthamii is recommended to perform the incision in the trees at the height of 20 and 50 cm above the ground, and new studies because not yielded results as the survival and rooting of cuttings
O objetivo do estudo foi resgatar, conservar e multiplicar o material genético das melhores espécies/procedências selecionadas de Eucalyptus spp., para a região norte de Santa Catarina e selecionar os melhores clones para a propagação em nível comercial. O povoamento de Eucalyptus spp. do estudo está localizada no município de Rio Negrinho, SC. O talhão foi plantado em dezembro de 1999. As espécies do gênero Eucalyptussp. presentes na área experimental são oriundas de sementes, provenientes de diversos locais. Para seleção das melhores procedências, os indivíduos foram avaliados quanto à sobrevivência, altura, diâmetro à altura do peito, volume, e características qualitativas como tortuosidade do tronco e bifurcação. Para o experimento de resgate de material vegetativo foramaplicadas as técnicas de anelamento e semianelamento em nove espécies potenciais, onde os indivíduos foram avaliados em termos de número de brotações, número de estacas, sobrevivência e enraizamento. O mesmo foi avaliado em função da juvenilidade através das diferentes alturas em relação ao solo para a E. benthamii. As espécies potencias em relação às variáveis quantitativas e qualitativas, assim como o ranking utilizado para selecionar as melhores espécies, as três espécies que se destacaram foram E. dunni. E. benthamiie E. dorrigoensis. Em relação as procedências para E. smithii a procedência de Wilson Promontorj, para E.benthamii a Austrália, assim como para E. deanei e E. viminalis a procedência Manville SC, e para E. macarthuriiPieter Maritz. Dentre os métodos de resgate vegetativo testados, o anelamento apresentou melhores resultados em função da emissão de brotações. Para a sobrevivência e o enraizamento dos propágulos torna-se necessário novos estudos uma vez que apenas E. deanei obteve 8 9 material enraizado com a utilização de AIB na concentração de 3.000 mg L-¹. Quanto a emissão de brotos em função do grau de juvenilidade para E. benthamii, recomenda-se realizar a incisão nas árvores na altura de 20 e 50 cm acima do solo, e a realização de novos estudos pois não obteve-se resultado quanto a sobrevivência e enraizamento das estacas
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Bordovský, Petr. "Vyhodnocení vlivu tlaku v komoře vzorku a velikosti clonek na výsledný tlak u scintilátoru detektoru pomocí systému Cosmos FloWorks." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2010. http://www.nusl.cz/ntk/nusl-218731.

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This work deal with the analysis of influence of pressure‘s sizes in vacuum chamber of specimen Evironmental Scanning Electron Microscope and the influence of sizes of aperture diaphragm by scintillation detector. The analysis proceeds in detector of secondary electrons. The detector is modelled by system 3D CAD SolidWorks with the help of system CAE Cosmos FloWorks.
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Lytle, Christian, and Christian Lytle. "Spectroscopy of Neutral Mercury in a Magneto-Optical Trap Based on a Novel Ytterbium Fiber-Amplified Cooling Laser Source." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/621471.

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In this dissertation I present experimental results obtained on the mercury optical clock project in the research group of Jason Jones at the University of Arizona. The project began in 2008 with the purpose of investigating the feasibility of neutral mercury as an optical clock species. The first series of investigations involved building the essential apparatus and scanning the doppler-broadened 6¹S₀ - 6³P₀ clock transition in ¹⁹⁹Hg. Here I present significant modifications to the cooling and trapping laser, improvements to the spectroscopy laser linewidth, and attempts to measure the 2-photon transition in ¹⁹⁹Hg. After previously demonstrating spectroscopy of the mercury clock transition using an optically-pumped semiconductor laser for the cooling and trapping source (OPSL), we replaced the OPSL with a a fiber-amplified ECLD system. We custom built a fiber amplifier to provide gain at 1015 nm, demonstrating the system can yield up to 5 W of signal power with excellent suppression of the ASE power. We find that the ASE is well suppressed by using a two-stage configuration and short sections of gain fiber. The linewidth of our original spectroscopy laser was over 10 kHz, which is unsuitable to resolve of sub-Doppler features. To enhance the performance of our spectroscopy system, we integrated faster feedback bandwidth using AOMs, and incorporated derivative gain into the system. This resulted in a feedback bandwidth for our spectroscopy laser of over 200 kHz. With this system, we demonstrate anactively stabilized linewidth of 525 Hz for our spectroscopy system. Using the upgraded cooling and spectroscopy laser systems, we demonstrate spectroscopy of the clock system and confirm temperature measurements derived from the transition linewidth. We also describe attempts to detect the recoil shift and 2-photon transition in neutral mercury.
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Albuquerque, Felipe de Alencar. "Detecção interprocedimental de clones semânticos." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/100/100131/tde-28012014-085446/.

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Fragmentos de código duplicado, ou clones, são inseridos em aplicativos por serem uma maneira simples de reúso, dentre outros motivos. Clones são, portanto, comuns em programas. No entanto, a atividade de manutenção pode ficar custosa se o código do programa analisado possuir muitos clones, principalmente os semânticos, os quais podem possuir códigos distintos, mas realizam tarefas similares. Nesse sentido, a utilização de ferramentas que automatizam a tarefa de detectar clones é desejável. Ferramentas atuais de detecção de clones semânticos são capazes de identificar esses clones com altas taxas de acerto. No entanto, elas não são capazes de identificar clones semânticos considerando também os fluxos dos procedimentos ou funções que são invocados dentro dos fragmentos de código comparados. Essa limitação pode levar as ferramentas a indicarem clones semânticos falso positivos. Este trabalho apresenta uma técnica de detecção de clones semânticos que considera as chamadas de procedimentos presentes nos programas. Essa técnica apresentou uma taxa de acertos 2,5% maior do que técnicas convencionais de acordo com um benchmark, também desenvolvido neste trabalho. Esse benchmark foi criado com base nas classificações de clones fornecidas por programadores da indústria e da academia. A técnica interprocedimental de detecção de clones semânticos pode ser utilizada para evolução, manutenção, refatoração e entendimento de programas.
Fragments of duplicated code, or clones, are embedded in applications as they are a simple way to reuse code, among other reasons. Clones are therefore common in programs. However, the maintenance activity may be costly if the program code has many clones to analyze, specially semantic clones, which are semantically similar but may have different syntax. In this regard, the use of tools that automate the task of detecting clones is desirable. Current tools for detecting semantic clones are able to identify those clones with high hit rates. However, they are not able to detect semantic clones also considering the flow of procedures or functions that are invoked within the compared code fragments. This limitation can lead the tools to indicate false positive semantic clones. This paper presents a technique that takes into account the procedure calls in programs to detect semantic clones. This technique showed a 2.5% higher hit rate than conventional techniques according to a benchmark also developed in this work. This benchmark was created based on evaluations provided by programmers from academic and industrial settings. The interprocedural semantic clone detection technique can be used for evolution, maintenance, refactoring and understanding of programs.
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Peszlen, Ilona. "Influence of site, clone, age, and growth rate on wood properties of three Populus X Euramericana clones." Diss., Virginia Tech, 1993. http://hdl.handle.net/10919/40146.

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Books on the topic "Clonky"

1

1940-, Smith Eric, ed. Clocks and clock repairing. 2nd ed. Blue Ridge Summit, PA: Tab Books, 1989.

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Centre Georges Pompidou. Espace 315., ed. Claude Closky. Paris: Centre Pompidou, 2006.

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Mitchell, A. Adjustments to the English clock systems: Master and slave clocks. [UK?]: Electrical Horology Group, Antiquarian Horological Society., 1986.

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Carrara, Guy. In vitro, ou, La légendes des clones =: Ou, A lenda dos clones = or, The clone legend. Montpellier: L'Entretemps, 2009.

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A modern tower clock installation: Including striking work. Petworth: The author, 1985.

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Barberito, Manlio. Roma, misura del tempo: Storie di orologi. Roma: Technimedia Wrist, 1994.

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Zeeman, J. De Nederlandse staande klok. Zwolle: Waanders, 1996.

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Clocks and more clocks. New York: Aladdin Books, 1994.

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Clocks and more clocks. New York: Macmillan, 1994.

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Gauthier, Michel. Claude Closky, 8002-9891. Vitry-sur-Seine: Mac-Val, 2008.

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Book chapters on the topic "Clonky"

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Saini, Vaibhav, Farima Farmahinifarahani, Hitesh Sajnani, and Cristina Lopes. "Oreo: Scaling Clone Detection Beyond Near-Miss Clones." In Code Clone Analysis, 63–74. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-1927-4_5.

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Agoyan, Michel, Jean-Max Dutertre, David Naccache, Bruno Robisson, and Assia Tria. "When Clocks Fail: On Critical Paths and Clock Faults." In Lecture Notes in Computer Science, 182–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-12510-2_13.

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Crussell, Jonathan, Clint Gibler, and Hao Chen. "Attack of the Clones: Detecting Cloned Applications on Android Markets." In Computer Security – ESORICS 2012, 37–54. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-33167-1_3.

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Cerny, Eduard, Surrendra Dudani, John Havlicek, and Dmitry Korchemny. "Clocks." In SVA: The Power of Assertions in SystemVerilog, 273–99. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-07139-8_12.

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Gangadharan, Sridhar, and Sanjay Churiwala. "Clocks." In Constraining Designs for Synthesis and Timing Analysis, 47–55. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-3269-2_5.

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Cerny, Eduard, Surrendra Dudani, John Havlicek, and Dmitry Korchemny. "Clocks." In The Power of Assertions in SystemVerilog, 269–94. Boston, MA: Springer US, 2010. http://dx.doi.org/10.1007/978-1-4419-6600-1_12.

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Beard, Ron, and Ken Senior. "Clocks." In Springer Handbook of Global Navigation Satellite Systems, 121–64. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-42928-1_5.

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Lotker, Zvi. "Clocks." In Analyzing Narratives in Social Networks, 187–213. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-68299-6_12.

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Reddy, Akhilesh B. "Genome-Wide Analyses of Circadian Systems." In Circadian Clocks, 379–88. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-25950-0_16.

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Heisler, Joel, Archana Chavan, Yong-Gang Chang, and Andy LiWang. "Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock." In Circadian Clocks, 3–18. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0381-9_1.

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Conference papers on the topic "Clonky"

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Li, Qi, and Jeffrey S. Vipperman. "Two-Dimensional Arbitrary Shape Acoustic Cloaks Composed of Homogeneous Parts Realized by Layered Structures." In ASME 2015 Noise Control and Acoustics Division Conference at InterNoise 2015. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/ncad2015-5924.

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Acoustic cloaking is an important application of metamaterials and has received much attention since it was first proposed. Due to the extreme properties of the cloaks produced by previous methods, they are difficult to fabricate. In addition, cloaks with arbitrary shapes are more favorable in applications but are difficult to realize. Therefore, it is important to present a method for designing arbitrary shaped cloak with attainable properties. In this paper, a technique for realizing cloaks with arbitrary shapes is presented by dividing the cloak into finite parts. Transformation acoustics is used to derive the properties of each part of the cloak. With appropriate mapping relationships, the properties of each part are anisotropic but homogeneous. Layered structures are adopted to approximate the anisotropic properties within each part. Full wave simulations are conducted to validate this technique. The method can be used to design cloaks with arbitrary shapes, which perform well within certain frequency limits. It provides an easier way to fabricate cloaks with arbitrary shapes.
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Hu, Jin, Xiaoming Zhou, and Gengkai Hu. "Design Arbitrary Shaped 2D Acoustic Cloak Without Singularity." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-10727.

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A method is proposed to design arbitrary shaped two dimensional (2D) isotropic-inertia acoustic cloaks without singularity. The method is based on the deformation view of the transformation method, where the transformation tensor A is identified as the deformation gradient tensor and the transformed material parameters can be expressed by the principal stretches in the principal system of the deformation. The infinite material parameters of a perfect 2D cloak is induced by an infinite principal stretch in one direction while the other two remains finite at the inner boundary during the transformation. To circumvent this difficulty, for a 2D cloak we can choose the principal stretch perpendicular to the cloak plane to be also infinite but in the same order as the infinite principal stretch in the cloak plane during the transformation, so the transformed material parameters may keep finite. To illustrate this idea, the analytical expressions of nonsingular material parameters for a cylindrical acoustic cloak are given. For the acoustic cloaks with irregular shapes, the numerical method is proposed to evaluate the principal stretches and in turn the nonsingular material parameters. The designed 2D cloaks are validated by numerical simulation.
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Wei, Hui-Hui, and Ming Li. "Positive and Unlabeled Learning for Detecting Software Functional Clones with Adversarial Training." In Twenty-Seventh International Joint Conference on Artificial Intelligence {IJCAI-18}. California: International Joint Conferences on Artificial Intelligence Organization, 2018. http://dx.doi.org/10.24963/ijcai.2018/394.

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Software clone detection is an important problem for software maintenance and evolution and it has attracted lots of attentions. However, existing approaches ignore a fact that people would label the pairs of code fragments as \emph{clone} only if they happen to discover the clones while a huge number of undiscovered clone pairs and non-clone pairs are left unlabeled. In this paper, we argue that the clone detection task in the real-world should be formalized as a Positive-Unlabeled (PU) learning problem, and address this problem by proposing a novel positive and unlabeled learning approach, namely CDPU, to effectively detect software functional clones, i.e., pieces of codes with similar functionality but differing in both syntactical and lexical level, where adversarial training is employed to improve the robustness of the learned model to those non-clone pairs that look extremely similar but behave differently. Experiments on software clone detection benchmarks indicate that the proposed approach together with adversarial training outperforms the state-of-the-art approaches for software functional clone detection.
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Studennikova, N. L., Z. V. Kotolovets, and R. G. Timofeev. "STUDY OF AGROBIOLOGICAL AND TECHNOLOGICAL FEATURES OF PERSPECTIVE CLONES OF GRAPES VARIETY MUSCAT ROSE." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.2.482-485.

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One of the promising varieties for the production of high-quality sparkling, dessert and liquor wines is the technical variety of pink muscat grapes. The aim of this work is to study the agrobiological and technological features of promising clones of the Pink Muscat variety. The object of study was two clones of the Pink Muscat variety - № 21-2-3 and № 53-10-2, growing on the clone test site (№ 509), the Gurzuf branch of the State Unitary Enterprise of the Republic of Kazakhstan PJSC Massandra on an area of 0.5 hectares. The article provides a statistical analysis of the quantitative characteristics of clones of the Muscat pink variety for 2018-2019, as well as the physicochemical and organoleptic characteristics of grapes and wine materials from clones of this variety of the 2019 crop. It was established that the fruiting coefficients (0.6- 0.65) in the studied clones are at the control level and are characterized as average. The mass of a bunch of clones exceeds the control by 1.4-2.15 times, reaching 322-494 g. The actual yield from the bush is 4.9-6.8 kg, exceeding the control by 2.13-2.96 times. According to the indicator, the shoot productivity in the wet mass of a bunch of 209.3-296.4 g /clone shoot is 1.68-2.38 times higher than the control. Wine materials made from clones of the Muscat pink grape varieties possess the amount of phenolic substances (within 506 mg /dm3 ) and the concentration of the reduced extract (23-25 g/dm3 ) at the control level, which gives grounds for further study of the selected clones in the technology of various types of wines . Tasting evaluation of wine materials also showed their high quality, not inferior to the wine material of the control sample.
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Spitzer, S. G., P. Usharani, A. D. Roser, C. K. Kasper, and S. G. Bajaj. "THE CATALYTIC TRIAD RESIDUES (HIS221, ASP269, SER365) AND THE BINDING POCKET RESIDUE (ASP359) IN FACTOR IXBm ELSINORE (IXBmLE) ARE NOT ALTERED." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644071.

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Previous studies suggested that the defect in IXBmLE (a nonfunctional variant of human IX) is either in the catalytic triad or at the site(s) of interaction with the macromolecular substrates (antithrombin III, factor VII or factor X). To distinguish between these possibilities, we isolated a complete IX cDNA clone from a human liver cDNA library. We also constructed a genomic library (in phage EMBL3) using DNA of the BmLE patient. The library was screened with normal IX cDNA and with synthetic oligonucleotides. The positive clones containing the exons coding for IX were plaque purified. Two clones which contained the coding sequence of the catalytic domain, i.e., His221 (exon VII), and Asp269, Asp359, and Ser365 (exon VIII) were selected for further studies. The phage containing exon VIII was first digested with Sail and EcoRI and a 2-Kb fragment, which hybridized with the segment of cDNA containing exon VIII, was gel purified. The 2-Kb fragment was further digested and the subfragments were cloned into M13; the length and direction of the fragments used in sequencing are shown below:The phage containing exon VII was digested with PstI and SalI, and a 1-Kb fragment that hybridized with the 19-mer His221 probe was subcloned into M13 phage for sequencing. The sequence starting with residue Vall96 through residue Arg403 was found to be normal. Thus, none of the residues in the catalytic domain of IXBmLE are different from that of normal IX. These data provide strong indirect evidence that the noncatalytic aminoterminal portion of IX plays a significant role in the structural recognition of the macromolecular substrates. The sequence of this region of IXBmLE should provide information about the putative residue(s) essential for this recognition.
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Liu, Mao, and W. D. Zhu. "Controlling Out-of-Plane Shear Wave Propagation With Broadband Cloaking." In ASME 2019 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/imece2019-12156.

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Abstract A major challenge in designing a perfect invisibility cloak for elastic waves is that density and elasticity tensors need to be independent functions of its radius with a linear transformation medium. The traditional cloak for out-of-plane shear waves in membranes exhibits material properties with inhomogeneous and anisotropic shear moduli and densities, which yields a poor or even negative cloaking efficiency. This paper presents design of a cylindrical cloak for shear waves based on a nonlinear transformation. This excellent broadband nonlinear cloak only requires variation of its shear modulus, while the density in the cloak region remains unchanged. The nonlinear ray trajectory equation for out-of-plane shear waves is derived and a parameter to adjust the efficiency of the cylindrical cloak is introduced. Qualities of the nonlinear invisibility cloak are discussed by comparison with those of a cloak with the linear transformation. Numerical examples show that the nonlinear cloak is more effective for shielding out-of-plane shear waves from outside the cloak than the linear cloak and illustrate that the nonlinear cloak for shear waves remains highly efficient in a broad frequency range. The proposed nonlinear transformation in conjunction with ray trajectory equations can also be used to design nonlinear cloaks for other elastic waves.
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Tehranian, Aref, and Sia Nemat-Nasser. "Acoustic and Elastic Cloaking Using Non-Singular Transformation." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65649.

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Non-singular transformation is developed to achieve approximate acoustic and elastic cloaking. In the literature, perfect cloaking is realized under the condition of mapping cloak inner boundary to a single point, which gives rise to the development of unconventional metamaterials. Such cloaks introduce serious challenges in terms of materials design and fabrication. This paper studies the transformation functions that map the inner boundary of a 2D acoustic or elastic cloak (in physical space) to a region with small but finite dimension (in mathematical space). The goal is to find an optimized transformation that considers the limitations on mass density anisotropy, and elastic anisotropy of realizable metamaterials. It should be noted that the proposed cloak is not perfect and scatters minimal portion of the incident wave. However, it provides a realistic path towards the design and fabrication of an acoustic cloak for pressure waves, and elastic cloak for general stress waves.
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Bao, Chunyan, and Liang-Wu Cai. "Use Multi-Objective Optimization for Designing Cloaks With Acoustic and Elastic Materials." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-88700.

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The various optimization schemes have been recently shown to be a capable tool for designing acoustic cloaks based on Cummer-Schurig prescription without requiring material singularity. However, when using an optimization scheme to optimize a cloak at one specified frequency, sometimes it is observed that the cloaking effect deteriorate at other frequencies. This paper explores the use of multi-objective optimization approach such that the cloaking performance is maintained over a wide range of frequencies. Two examples are presented. The first cloak is comprised of all aocustic layers, and the second cloak is comprised of a mixture of acoustic and elastic layers. The results show the effectiveness of the multi-objective optimization approach on maintaining cloaking performance over a specified frequency range.
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Ploos van Amstel, J. K., A. L. van der Zanden, E. Bakker, P. H. Reitsma, and R. M. Bertina. "INDEPENDENT ISOLATION OF HUMAN PROTEIN S cDNA AND THE ASSIGNMENT OF THE GENE TO CHROMOSOME 3." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644638.

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Protein S is a vitamin K-dependent glycoprotein, that serves as a cofactor of activated protein C. A hereditary deficiency in protein S is associated with an increased risk for the development of venous thrombosis. The deficiency is inherited as an autosomal dominant trait. We isolated a cDNA coding for protein S and assigned its gene to chromosome 3.A human liver cDNA library in phage xgtll (complexity 1.2 × 106 , D. Stafford, Chapel Hill) was screened by using immuno-purifiedpolyclonal anti-protein S IgG as a probe. Approximately 1.5 x 10 recombinants of the amplified library were screened. Out of eighteen positive clones one clone was found, after nucleotide sequence analysis, to code for a peptide with a high degree of homology with the carboxy terminal region of the already published bovine protein S. This clone pSP84 (450 bp) was used as a probe to screen a human liver cDNA library in plasmid pUC9. From this library we isolated several positive clones. Clone pSUL5 contained the largest insert (2200 base pairs). Dideoxy sequencing revealed that it codes for 330 amino acids of the carboxy terminal part of protein S. Furthermore, it contained a 1200 base pairs 3' untranslated region. The predicted amino acid sequence did not differ from the published sequence of human protein S, although at the nucleotide level some differences could be detected.Clone pSUL5 was used to localize the protein S gene to its chromosome. The assignment was done by hybridization to Pst I digested DNA from human-hamster c.q. human-mouse somatic cell hybrids. In this way we got strong indication that the protein S gene is located on chromosome 3.
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Uzan, G., A. Lajmanovich, M. H. Prandini, Ph Frachet, A. Duperray, and G. Marguerie. "MOLECULAR CLONING OF PLATELET GPIIb FROM HEL CELLS AND HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643960.

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Platelet GP IIb-IIIa is an heterodimer which functions as a receptor for fibrinogen, fibronectin and Von Willebrand factor and is implicated in platelet adhesive reactions. To study the structure function relationship of this glycoprotein, a recombinant DNA approach was initiated. cDNA expression libraries were constructed in » gtll vector, from erythro-leukemia cells (HEL) and megakaryocytes mRNA. The human megakaryocytes were isolated from patients with chronic myeloid leukemia. The HEL library was initially screened with polyclonal antibodies anti GPIIb IIIa. One clone, λIIbI, containing a 1.65 kbp insert reacted with a panel of different polyclonal antibodies anti GPIIb IIIa and a monoclonal antibody anti GPIIb. To further characterize this clone the synthesis of the fusion protein was induced by IPTG. The bacterial protein was then blotted onto nitro cellulose and incubated with antisera anti GPIIb-IIIa. Antibodies that specifically bound with the fusion protein were eluted and tested on platelet membrane extracts. The selected antibodies produced a positive signal at the GPIIb position similar to the signal produced by the monoclonal antibody anti GPIIb on the same membrane extract. Finally on western blotting, a protein of Mr= 170kD reacted with the monoclonal antibody anti GPIIb. λIIbI insert was used to screen the megakaryocyte library and 3 clones, λIIb2,λIIb3 and λIIb4 were isolated. The size of HEL cells and megakaryocytes GPIIb mRNA was estimated by northern blotting. Only one species of 3.9 kb was identified in both cells. The four different clones accounted for 50% of the coding sequence of this mRNA.Sequencing of these cDNAs indicated that the plasmatic domain of GPIIb contains a cystein rich region. The sequence of these clones will allow the study of the adhesines genetic diversity in different cellular systems.
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Reports on the topic "Clonky"

1

Arnett, Clint, Justin Lange, Ashley Boyd, Martin Page, and Donald Cropek. Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41546.

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Cationic polypeptide proteins found in the seeds of the tropical plant Moringa oleifera have coagulation efficiencies similar to aluminum and ferric sulfates without their recalcitrant nature. Although these proteins possess great potential to augment or replace traditional coagulants in water treatment, harvesting active protein from seeds is laborious and not cost-effective. Here, we describe an alternative method to express and secrete active M. oleifera coagulant protein (MO) in Bacillus subtilis. A plasmid library containing the MO gene and 173 different types of secretory signal peptides was created and cloned into B. subtilis strain RIK1285. Fourteen of 440 clones screened were capable of secreting MO with yields ranging from 55 to 122 mg/L of growth medium. The coagulant activity of the highest MO secreting clone was evaluated when grown on Luria broth, and cell-free medium from the culture was shown to reduce turbidity in a buffered kaolin suspension by approximately 90% compared with controls without the MO gene. The clone was also capable of secreting active MO when grown on a defined synthetic wastewater supplemented with 0.5% tryptone. Cell-free medium from the strain harboring the MO gene demonstrated more than a 2-fold reduction in turbidity compared with controls. Additionally, no significant amount of MO was observed without the addition of the synthetic wastewater, suggesting that it served as a source of nutrients for the effective expression and translocation of MO into the medium.
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Royette Tavernier, Ph.D., Royette Tavernier, Ph D. Battle of the clocks: Does your biological clock determine whether the timing of exercise impairs or promotes sleep? Experiment, October 2016. http://dx.doi.org/10.18258/8234.

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Buisson, James A., and Marie M. Largay. On-Orbit Frequency Stability Analysis of the GPS NAVSTAR 8 Rubidium Clock and NAVSTARs 9 and 10 Cesium Clocks. Fort Belvoir, VA: Defense Technical Information Center, September 1987. http://dx.doi.org/10.21236/ada184984.

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Cline, Sue. Correlation of Protocol Clocks. Fort Belvoir, VA: Defense Technical Information Center, July 1988. http://dx.doi.org/10.21236/ada207843.

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Lombardi, Michael A., Andrew N. Novick, John P. Lowe, Matthew J. Deutch, Glenn K. Nelson, Douglas S. Sutton, and William C. Yates. WWVB radio controlled clocks :. Gaithersburg, MD: National Institute of Standards and Technology, 2005. http://dx.doi.org/10.6028/nist.sp.960-14e2005.

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Jespersen, James, and Jane Fitz-Randolph. From sundials to atomic clocks :. Gaithersburg, MD: National Institute of Standards and Technology, 1999. http://dx.doi.org/10.6028/nbs.mono.155e1999.

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Mills, D. L. Algorithms for synchronizing network clocks. RFC Editor, September 1985. http://dx.doi.org/10.17487/rfc0956.

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Leibowitz, Michael J. Translational Regulation of Cloned Genes. Fort Belvoir, VA: Defense Technical Information Center, February 1992. http://dx.doi.org/10.21236/ada248892.

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Allan, D. W., David A. Howe, F. L. Walls, and D. B. Sullivan. Characterization of clocks and oscillators. Gaithersburg, MD: National Bureau of Standards, 1990. http://dx.doi.org/10.6028/nist.tn.1337.

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Suess, Matthias, Demetrios Matsakis, and Charles A. Greeenhall. Simulating Future GPS Clock Scenarios with Two Composite Clock Algorithms. Fort Belvoir, VA: Defense Technical Information Center, November 2010. http://dx.doi.org/10.21236/ada547035.

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