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Journal articles on the topic 'Closed microfluidic system'

Author: Grafiati

Published: 18 May 2024

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1

Debski, Pawel, Karolina Sklodowska, Jacek Michalski, Piotr Korczyk, Miroslaw Dolata, and Slawomir Jakiela. "Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures." Micromachines 9, no. 9 (2018): 469. http://dx.doi.org/10.3390/mi9090469.

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Emerging microfluidic technology has introduced new precision controls over reaction conditions. Owing to the small amount of reagents, microfluidics significantly lowers the cost of carrying a single reaction. Moreover, in two-phase systems, each part of a dispersed fluid can be treated as an independent chemical reactor with a volume from femtoliters to microliters, increasing the throughput. In this work, we propose a microfluidic device that provides continuous recirculation of droplets in a closed loop, maintaining low consumption of oil phase, no cross-contamination, stabilized temperatu

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Steege, Tobias, Mathias Busek, Stefan Grünzner, Andrés Fabían Lasagni, and Frank Sonntag. "Closed-loop control system for well-defined oxygen supply in micro-physiological systems." Current Directions in Biomedical Engineering 3, no. 2 (2017): 363–66. http://dx.doi.org/10.1515/cdbme-2017-0075.

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AbstractTo improve cell vitality, sufficient oxygen supply is an important factor. A deficiency in oxygen is called Hypoxia and can influence for example tumor growth or inflammatory processes. Hypoxia assays are usually performed with the help of animal or static human cell culture models. The main disadvantage of these methods is that the results are hardly transferable to the human physiology. Microfluidic 3D cell cultivation systems for perfused hypoxia assays may overcome this issue since they can mimic the in-vivo situation in the human body much better. Such a Hypoxia-on-a-Chip system w

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Wang, Ningquan, Ruxiu Liu, Norh Asmare, Chia-Heng Chu, Ozgun Civelekoglu, and A. Fatih Sarioglu. "Closed-loop feedback control of microfluidic cell manipulation via deep-learning integrated sensor networks." Lab on a Chip 21, no. 10 (2021): 1916–28. http://dx.doi.org/10.1039/d1lc00076d.

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4

Loutherback, K., P. A. Bulur, and A. Dietz. "Process Development and Manufacturing: CLOSED MICROFLUIDIC SYSTEM FOR MANUFACTURING DENDRITIC CELL THERAPIES." Cytotherapy 24, no. 5 (2022): S171—S172. http://dx.doi.org/10.1016/s1465-3249(22)00448-0.

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Loutherback, K., P. A. Bulur, and A. Dietz. "Process Development and Manufacturing: CLOSED MICROFLUIDIC SYSTEM FOR MANUFACTURING DENDRITIC CELL THERAPIES." Cytotherapy 24, no. 5 (2022): S171—S172. http://dx.doi.org/10.1016/s1465-3249(22)00448-0.

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6

Fu, Hai, Wen Zeng, Songjing Li, and Shuai Yuan. "Electrical-detection droplet microfluidic closed-loop control system for precise droplet production." Sensors and Actuators A: Physical 267 (November 2017): 142–49. http://dx.doi.org/10.1016/j.sna.2017.09.043.

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7

Hansen, J. S., J. T. Ottesen, and A. Lemarchand. "Molecular dynamics simulations of valveless pumping in a closed microfluidic tube-system." Molecular Simulation 31, no. 14-15 (2005): 963–69. http://dx.doi.org/10.1080/08927020500419297.

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Yafia, Mohamed, Amir M. Foudeh, Maryam Tabrizian, and Homayoun Najjaran. "Low-Cost Graphene-Based Digital Microfluidic System." Micromachines 11, no. 9 (2020): 880. http://dx.doi.org/10.3390/mi11090880.

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In this work, the laser-scribing technique was used as a low-cost, rapid and facile method for fabricating digital microfluidic (DMF) systems. Laser-scribed graphene (LSG) electrodes are directly synthesized on flexible substrates to pattern the DMF electrode arrays. This facilitates the DMF electrodes’ fabrication process by eliminating many microfabrication steps. An electrowetting test was performed to investigate the effectiveness of the LSG DMF electrodes in changing the contact angles of droplets. Different DMF operations were successfully performed using the proposed LSG DMF chips in bo

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Lim, Hyunjung, Jae Young Kim, Seunghee Choo, et al. "Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics." Micromachines 14, no. 4 (2023): 712. http://dx.doi.org/10.3390/mi14040712.

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An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. The total sample preparation system contains two-step microfluidic devices: a closed-loop separation and concentration device and a co-flow cell-washing device. To determine the flow conditions of the closed-loop device, such as the flow rate factor, a mixture of 4 and 13 μm particles was used. C

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Jang, Kihoon, Yan Xu, Yo Tanaka, et al. "Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system." Biomicrofluidics 4, no. 3 (2010): 032208. http://dx.doi.org/10.1063/1.3494287.

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11

Kim, Jeeyong, Hyunjung Lim, Hyunseul Jee, et al. "High-Throughput Cell Concentration Using A Piezoelectric Pump in Closed-Loop Viscoelastic Microfluidics." Micromachines 12, no. 6 (2021): 677. http://dx.doi.org/10.3390/mi12060677.

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Cell concentration is a critical process in biological assays and clinical diagnostics for the pre-treatment of extremely rare disease-related cells. The conventional technique for sample preconcentration and centrifugation has the limitations of a batch process requiring expensive and large equipment. Therefore, a high-throughput continuous cell concentration technique needs to be developed. However, in single-pass operation, the required concentration ratio is hard to achieve. In this study, we propose a closed-loop continuous cell concentration system using a viscoelastic non-Newtonian flui

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Kim, Jeong, Hye Choi, Chul Kim, Hee Jin, Jae-sung Bae, and Gyu Kim. "Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel." Micromachines 9, no. 10 (2018): 482. http://dx.doi.org/10.3390/mi9100482.

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With increasing interest in induced pluripotent stem cells (iPSCs) in the field of stem cell research, highly efficient infection of somatic cells with virus factors is gaining importance. This paper presents a method of employing microfluidic devices for dynamic cell culture and virus infection in a microchannel. The closed space in the microchannel provided a better environment for viruses to diffuse and contact cell surfaces to infect cells. The microfluidic devices were fabricated by photolithography and soft lithography. NIH/3T3 fibroblast cells were cultured in the microfluidic device in

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Heuck, F., P. van der Ploeg, and U. Staufer. "Deposition and structuring of Ag/AgCl electrodes inside a closed polymeric microfluidic system for electroosmotic pumping." Microelectronic Engineering 88, no. 8 (2011): 1887–90. http://dx.doi.org/10.1016/j.mee.2011.01.058.

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14

Bohm, Sebastian, and Erich Runge. "Multiphysics simulation of fluid interface shapes in microfluidic systems driven by electrowetting on dielectrics." Journal of Applied Physics 132, no. 22 (2022): 224702. http://dx.doi.org/10.1063/5.0110149.

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We present a highly efficient simulation method for the calculation of three-dimensional quasi-static interface shapes under the influence of electric fields. The method is especially useful for the simulation of microfluidic systems driven by electrowetting on dielectrics because it accounts automatically and inherently for the highly non-trivial interface shape in the vicinity of the triple-phase contact. In particular, the voltage independence of the local contact angle predicted based on analytical considerations is correctly reproduced in all our simulations. For the calculation of the sh

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Tremblay, Yannick D. N., Philippe Vogeleer, Mario Jacques, and Josée Harel. "High-Throughput Microfluidic Method To Study Biofilm Formation and Host-Pathogen Interactions in Pathogenic Escherichia coli." Applied and Environmental Microbiology 81, no. 8 (2015): 2827–40. http://dx.doi.org/10.1128/aem.04208-14.

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ABSTRACTBiofilm formation and host-pathogen interactions are frequently studied using multiwell plates; however, these closed systems lack shear force, which is present at several sites in the host, such as the intestinal and urinary tracts. Recently, microfluidic systems that incorporate shear force and very small volumes have been developed to provide cell biology models that resemblein vivoconditions. Therefore, the objective of this study was to determine if the BioFlux 200 microfluidic system could be used to study host-pathogen interactions and biofilm formation by pathogenicEscherichia

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Fan, Shangchun, Jinhao Sun, Weiwei Xing, Cheng Li, and Dongxue Wang. "Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling." Journal of Applied Mathematics 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/378253.

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We report a principle prototype of space animal cell perfusion culture and observation. Unlike previous work, our cell culture system cannot only realize microfluidic and temperature controlling, automatic observation, and recording but also meet an increasing cell culture at large scale operation and overcome shear force for animal cells. A key component in the system is ingenious structural fused silica cell culture cavity with the wedge-shaped connection. Finite volume method (FVM) is applied to calculate its multipoint flow field, pressure field, axial velocity, tangential velocity, and ra

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Alrifaiy, Ahmed, and Kerstin Ramser. "How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte." Biomedical Optics Express 2, no. 8 (2011): 2299. http://dx.doi.org/10.1364/boe.2.002299.

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Guan, Yin, Baiyun Li, and Lu Xing. "Numerical investigation of electrowetting-based droplet splitting in closed digital microfluidic system: Dynamics, mode, and satellite droplet." Physics of Fluids 30, no. 11 (2018): 112001. http://dx.doi.org/10.1063/1.5049511.

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19

Kimura, Hiroshi, Hirokazu Takeyama, Kikuo Komori, Takatoki Yamamoto, Yasuyuki Sakai, and Teruo Fujii. "Microfluidic Device with Integrated Glucose Sensor for Cell-Based Assay in Toxicology." Journal of Robotics and Mechatronics 22, no. 5 (2010): 594–600. http://dx.doi.org/10.20965/jrm.2010.p0594.

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We have developed a cell-based assay platform using a microfluidic device integrating a glucose sensor into a cell culture device with closed-loop perfusion. Online measurement of cell kinetic change associated with cell status change was achieved by measuring glucose concentration change in the device with a cell exposed to a toxic material. The cell-based assay platform, which is integrated with a sensor and a perfusion system, was expected to improve measurement accuracy and efficiency, leading to the discovery of new tools in such wide-ranging fields as drug discovery, life sciences, and m

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Bartsch de Torres, Heike, Christian Rensch, Torsten Thelemann, J. Müller, and M. Hoffmann. "Fully Integrated Bridge-Type Anemometer in LTCC-Based Microfluidic Systems." Advances in Science and Technology 54 (September 2008): 401–4. http://dx.doi.org/10.4028/www.scientific.net/ast.54.401.

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A thick film anemometer for in situ control of the flow rate in fluidic systems was designed, manufactured and characterized. The sensor is integrated in a retention modulus consisting of Low Temperature Cofired Ceramics (LTCC). These materials allow the cost-effective realisation of fluidic microsystems with integrated electronics. The challenge of the work is to design an anemometer under the exclusive use of thick film technologies. The necessity to trim resistors causes the external use of relevant pastes. Therefore, the use inside of a closed fluidic system requires the leak of process ga

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Nouri, Abdelmounaim, Maria L. Rodgers, Daniel L. Bolnick, et al. "Microfluidic gut-on chip system for reproducing the microbiome-immune cells interaction in Threespine Stickleback." Journal of Immunology 208, no. 1_Supplement (2022): 116.05. http://dx.doi.org/10.4049/jimmunol.208.supp.116.05.

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Abstract The development of in vitro models that mimic the immunological, mechanical, and structural properties of the gut, as well as its crucial microbial symbionts, facilitate the real-time assessment of host-microbiome interactions. Here, we describe our development of a threespine stickleback fish “gut-on-chip” biomimetic microdevice which will co-culture complex microbial communities with mucus-secreting intestinal epithelia and leukocytes. This chip was fabricated using soft lithography with liquid PDMS and consists of a middle channel coated with extracellular matrix (ECM) compounds se

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22

Heidt, Benjamin, Renato Rogosic, Nils Leoné, et al. "Topographical Vacuum Sealing of 3D-Printed Multiplanar Microfluidic Structures." Biosensors 11, no. 10 (2021): 395. http://dx.doi.org/10.3390/bios11100395.

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We demonstrate a novel way of creating three-dimensional microfluidic channels capable of following complex topographies. To this end, substrates with open channels and different geometries were 3D-printed, and the open channels were consecutively closed with a thermoplastic using a low-resolution vacuum-forming approach. This process allows the sealing of channels that are located on the surface of complex multiplanar topographies, as the thermoplastic aligns with the surface-shape (the macrostructure) of the substrate, while the microchannels remain mostly free of thermoplastic as their smal

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Koenig, Leopold, Anja Patricia Ramme, Daniel Faust, et al. "A Human Stem Cell-Derived Brain-Liver Chip for Assessing Blood-Brain-Barrier Permeation of Pharmaceutical Drugs." Cells 11, no. 20 (2022): 3295. http://dx.doi.org/10.3390/cells11203295.

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Significant advancements in the field of preclinical in vitro blood-brain barrier (BBB) models have been achieved in recent years, by developing monolayer-based culture systems towards complex multi-cellular assays. The coupling of those models with other relevant organoid systems to integrate the investigation of blood-brain barrier permeation in the larger picture of drug distribution and metabolization is still missing. Here, we report for the first time the combination of a human induced pluripotent stem cell (hiPSC)-derived blood-brain barrier model with a cortical brain and a liver spher

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Schmieder, Florian, Stefan Behrens, Nina Reustle, et al. "A microphysiological system to investigate the pressure dependent filtration at an artificial glomerular kidney barrier." Current Directions in Biomedical Engineering 5, no. 1 (2019): 389–91. http://dx.doi.org/10.1515/cdbme-2019-0098.

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AbstractChronic kidney disease (CKD) is a global health problem that affects around 11 to 13% of the world’s population and more than 18% of European citizens. Characteristic syndromes of CKD during all stages of the disease are proteinuria and ongoing glomerular dysfunction caused by cellular damages at the glomerular filtration barrier. While some rare cases of the disease are correlated to genetic depositions the majority of cases are caused by diabetes, glomerulosclerosis, high blood pressure and glomerulonephritis. Thus, recapitulating the interplay of high blood pressure and changes at t

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Gómez, J. R., J. P. Escandón, C. G. Hernández, R. O. Vargas, and D. A. Torres. "Multilayer analysis of immiscible power-law fluids under magnetohydrodynamic and pressure-driven effects in a microchannel." Physica Scripta 96, no. 12 (2021): 125028. http://dx.doi.org/10.1088/1402-4896/ac37a0.

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Abstract In the present study, the combined magnetohydrodynamic and pressure-driven flow of multilayer immiscible fluids into a parallel flat plate microchannel is semi-analytically solved. Due to the handling of complex fluids in various microfluidic platform applications, the fluid transport reviewed here considers the power-law model. The movement of electrically conductive fluid layers is due to Lorentz forces that arise from the interaction between an electric current and a magnetic field. To find a solution for the flow field, the momentum equation and the rheological model for each flui

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Silverio, Vania, Miguel Amaral, João Gaspar, Susana Cardoso, and Paulo P. Freitas. "Manipulation of Magnetic Beads with Thin Film Microelectromagnet Traps." Micromachines 10, no. 9 (2019): 607. http://dx.doi.org/10.3390/mi10090607.

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Integration of point-of-care assays can be facilitated with the use of actuated magnetic beads (MB) to perform testing in less expensive settings to enable the delivery of cost-effective care. In this paper we present six different designs of planar microelectromagnets traps (MEMT) with four external coils in series and one central coil connected for an opposite direction of manipulation of MB in microfluidic flows. The development of a simulation tool facilitated the rapid and efficient optimization of designs by presenting the influence of system variables on real time concentrations of MB.

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Zhang, Bailin, Juan Manuel Tamez-Vela, Steven Solis, et al. "Detection of Myoglobin with an Open-Cavity-Based Label-Free Photonic Crystal Biosensor." Journal of Medical Engineering 2013 (June 2, 2013): 1–7. http://dx.doi.org/10.1155/2013/808056.

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The label-free detection of one of the cardiac biomarkers, myoglobin, using a photonic-crystal-based biosensor in a total-internal-reflection configuration (PC-TIR) is presented in this paper. The PC-TIR sensor possesses a unique open optical microcavity that allows for several key advantages in biomolecular assays. In contrast to a conventional closed microcavity, the open configuration allows easy functionalization of the sensing surface for rapid biomolecular binding assays. Moreover, the properties of PC structures make it easy to be designed and engineered for operating at any optical wav

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Sakurai, Yumiko, Elaissa T. Hardy, Byungwook Ahn, et al. "Engineering a Valve-Regulated Endothelialized Microfluidic Device As an "in Vitro" Bleeding Time for Assessing Global Hemostasis." Blood 126, no. 23 (2015): 3485. http://dx.doi.org/10.1182/blood.v126.23.3485.3485.

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Abstract Introduction: Hemostasis encompasses an ensemble of regulated and balanced interactions among platelets (and other blood cells), coagulation factors, the endothelium, and the hemodynamic forces of the circulation. Unfortunately, current bleeding assays assess only isolated aspects of this complex process. A single assay that incorporates all of the major components of hemostasis would be of significant value in the research and clinical settings. To that end, we leveraged our laboratory's expertise in microfluidic systems to construct an "in vitro" bleeding time. Specifically, we deve

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Busek, Mathias, Mario Schubert, Kaomei Guan, et al. "Microphysiological system for heart tissue - going from 2D to 3D culture." Current Directions in Biomedical Engineering 5, no. 1 (2019): 269–72. http://dx.doi.org/10.1515/cdbme-2019-0068.

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AbstractRecently, we could demonstrate positive effects of microfluidic cultivation conditions on maturation of cardiomyocytes derived from human induced pluripotent stem cells (iPS-CMs) in a 2D model. However, 3D cell culture models are much closer to physiological conditions. Combined with microfluidics, 3D systems should resemble the in-vivo conditions even better than standard 2D cultivation. For 3D models, two main technical challenges arise, the tissue integration and sufficient nutrient supply for the cells. This work focuses on concepts for the tissue integration based on a modular app

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Mu, Ruojun, Nitong Bu, Jie Pang, Lin Wang, and Yue Zhang. "Recent Trends of Microfluidics in Food Science and Technology: Fabrications and Applications." Foods 11, no. 22 (2022): 3727. http://dx.doi.org/10.3390/foods11223727.

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The development of novel materials with microstructures is now a trend in food science and technology. These microscale materials may be applied across all steps in food manufacturing, from raw materials to the final food products, as well as in the packaging, transport, and storage processes. Microfluidics is an advanced technology for controlling fluids in a microscale channel (1~100 μm), which integrates engineering, physics, chemistry, nanotechnology, etc. This technology allows unit operations to occur in devices that are closer in size to the expected structural elements. Therefore, micr

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Weislogel, Mark M., J. Alex Baker, and Ryan M. Jenson. "Quasi-steady capillarity-driven flows in slender containers with interior edges." Journal of Fluid Mechanics 685 (September 23, 2011): 271–305. http://dx.doi.org/10.1017/jfm.2011.314.

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AbstractIn the absence of significant body forces the passive manipulation of fluid interfacial flows is naturally achieved by control of the specific geometry and wetting properties of the system. Numerous ‘microfluidic’ systems on Earth and ‘macrofluidic’ systems aboard spacecraft routinely exploit such methods and the term ‘capillary fluidics’ is used to describe both length-scale limits. In this work a collection of analytic solutions is offered for passive and weakly forced flows where a bulk capillary liquid is slowly drained or supplied by a faster capillary flow along at least one inte

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Cantwell, Christy, John S. McGrath, Clive A. Smith, and Graeme Whyte. "Image-Based Feedback of Multi-Component Microdroplets for Ultra-Monodispersed Library Preparation." Micromachines 15, no. 1 (2023): 27. http://dx.doi.org/10.3390/mi15010027.

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Using devices with microfluidic channels can allow for precise control over liquids flowing through them. Merging flows of immiscible liquids can create emulsions with highly monodispersed microdroplets within a carrier liquid, which are ideal for miniaturised reaction vessels which can be generated with a high throughput of tens of thousands of droplets per second. Control of the size and composition of these droplets is generally performed by controlling the pumping system pushing the liquids into the device; however, this is an indirect manipulation and inadequate if absolute precision is r

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Wuchter, Patrick, Rainer Saffrich, Stefan Giselbrecht, Anthony D. Ho, and Eric Gottwald. "Novel 3D-Model for the Hematopoietic Stem Cell Niche Using MSC in a KITChip Based Bioreactor." Blood 118, no. 21 (2011): 1331. http://dx.doi.org/10.1182/blood.v118.21.1331.1331.

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Abstract Abstract 1331 Human mesenchymal stromal cells (MSC) maintain “stemness” of human hematopoietic stem cells (HSC) by cell-cell contact when used as feeder-layer. We previously demonstrated the presence of specific cadherin-catenin-based junctions between HSC and MSC in a two-dimensional coculture-setting. To develop a more physiological in vitro model for the hematopoietic stem cell niche, we have established a novel 3D-coculture-system based on the so-called 3D-KITChip capable of accommodating up to 1×107 MSC on an area of ∼2cm2. For a precise reproduction of the microenvironment of th

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Tran, Reginald, David R. Myers, Jordan E. Shields, et al. "Improving Lentiviral Transduction Efficiency with Microfluidic Systems." Blood 126, no. 23 (2015): 4415. http://dx.doi.org/10.1182/blood.v126.23.4415.4415.

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Abstract Background: Recent clinical trials have demonstrated the efficacy and safety of gene therapy utilizing HIV-derived lentiviral vectors (LVs) for blood disorders. However, the LV requirements and clinical ex vivo cell transduction protocols used in these studies exposes the limitations of the technology and beckons the need for improved LV manufacturing and clinical transduction efficiency. Many methods have been devised to enhance efficiency, although none have circumvented the exorbitant amounts of virus required to achieve therapeutic HSC transduction. Furthermore, prolonged ex vivo

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Raub, Aini Ayunni Mohd, Ida Hamidah, Asep Bayu Dani Nandiyanto, et al. "ZnO NRs/rGO Photocatalyst in a Polymer-Based Microfluidic Platform." Polymers 15, no. 7 (2023): 1749. http://dx.doi.org/10.3390/polym15071749.

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This paper reports the development of ZnO NRs/rGO-based photocatalysts integrated into a tree-branched polymer-based microfluidic reactor for efficient photodegradation of water contaminants. The reactor system includes a photocatalytic reactor, tree-branched microfluidic channels, and ZnO nanorods (NRs) coated with reduced graphene oxide (rGO) on a glass substrate within an area of 0.6 × 0.6 cm2. The ZnO NRs/rGO acts as a photocatalyst layer grown hydrothermally and then spray-coated with rGO. The microfluidic system is made of PDMS and fabricated using soft lithography (micro molding using S

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Rehmani, Muhammad Asif Ali, Swapna A. Jaywant, and Khalid Mahmood Arif. "Study of Microchannels Fabricated Using Desktop Fused Deposition Modeling Systems." Micromachines 12, no. 1 (2020): 14. http://dx.doi.org/10.3390/mi12010014.

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Microfluidic devices are used to transfer small quantities of liquid through micro-scale channels. Conventionally, these devices are fabricated using techniques such as soft-lithography, paper microfluidics, micromachining, injection moulding, etc. The advancement in modern additive manufacturing methods is making three dimensional printing (3DP) a promising platform for the fabrication of microfluidic devices. Particularly, the availability of low-cost desktop 3D printers can produce inexpensive microfluidic devices in fast turnaround times. In this paper, we explore fused deposition modellin

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Stella, Giovanna, Lorena Saitta, Alfredo Edoardo Ongaro, Gianluca Cicala, Maïwenn Kersaudy-Kerhoas, and Maide Bucolo. "Advanced Technologies in the Fabrication of a Micro-Optical Light Splitter." Micro 3, no. 1 (2023): 338–52. http://dx.doi.org/10.3390/micro3010023.

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In microfluidics, it is important to confine and transport light as close as possible to the sample by guiding it into a small volume of the microfluidic channel, acquiring the emitted/transmitted radiation. A challenge in this context is the miniaturization of the optical components and their integration into the microfluidic device. Among all of the optical components, a particular role is played by the beam splitter, an important optical device capable of splitting light into several paths. In this paper, a micro-splitter is designed and realized by exploiting low-cost technologies. The mic

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Wang, Weiqiang, and Thomas B. Jones. "Moving droplets between closed and open microfluidic systems." Lab on a Chip 15, no. 10 (2015): 2201–12. http://dx.doi.org/10.1039/c5lc00014a.

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Nguyen, Duong Thanh, Van Thi Thanh Tran, Huy Trung Nguyen, Hong Thi Cao, Thai Quoc Vu, and Dung Quang Trinh. "Preparation of microfluidics device from PMMA for liposome synthesis." Vietnam Journal of Science and Technology 61, no. 1 (2023): 84–90. http://dx.doi.org/10.15625/2525-2518/16577.

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Microfluidics has emerged in recent years as a technology that has advantages and is well suited for studying chemistry, biology, and physics at the microscale. A common material which has been widely use to fabricate the microfluidic system is thermoplastic materials. The method of fabricating microfluidic devices has been growing because of advantages such as high-quality feature replication, inexpensiveness, and ease of use. However, the major barrier to the utilization of thermoplastics is the lack of bonding methods for different plastic layers to close the microchannels. Therefore, this

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Pinck, Stéphane, Lucila Martínez Ostormujof, Sébastien Teychené, and Benjamin Erable. "Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms." Microorganisms 8, no. 11 (2020): 1841. http://dx.doi.org/10.3390/microorganisms8111841.

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It is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to understand that bioelectrochemical systems (BESs) have been miniaturized into microBES by taking advantage of the worldwide development of microfluidics. Microfluidics tools applied to bioelectrochemistry permit even more fundamental studies of interactions and coupled phenomena occurring at the microscale, thanks, in particular, to the concomitant combin

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Behmardi, Yasna, Laurissa Ouaguia, Laura Jean Healey, et al. "Deterministic Cell Separation Recovers >2-Fold T Cells, and More Naïve T Cells, for Autologous Cell Therapy As Compared to Centrifugally Prepared Cells." Blood 138, Supplement 1 (2021): 2847. http://dx.doi.org/10.1182/blood-2021-153528.

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Abstract CAR-T autologous cell therapies are delivering impressive results in the clinic. However, there are still significant manufacturing challenges impeding the rapid adoption of these advanced therapies. On the first day of cell processing, most manufacturing approaches require ~5 steps (~4 hours) to obtain a white blood cell (WBC) preparation sufficiently depleted of red blood cells (RBCs) for T-cell selection and activation steps; and involves large cell losses and a great deal of inconsistency. Here we present a single-step procedure that yields &gt;2 fold more cells that centrifug

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Soenksen, L. R., T. Kassis, M. Noh, L. G. Griffith, and D. L. Trumper. "Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing." Lab on a Chip 18, no. 6 (2018): 902–14. http://dx.doi.org/10.1039/c7lc01223c.

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Nasibullayev, I. Sh, and O. V. Darintsev. "Two-dimensional dynamic model of the interaction of a fluid and a piezoelectric bending actuator in a plane channel." Multiphase Systems 14, no. 4 (2019): 220–32. http://dx.doi.org/10.21662/mfs2019.4.029.

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The paper proposes a two-dimensional computer model of the fluid flow in a plane channel under the action of an applied pressure drop with a flat ceramic piezoelectric actuator immersed in it, bending in the transverse direction in proportion to the applied electric voltage. A mathematical model of the coupled liquid-piezodrive system in variational form for numerical simulation by the finite element method is proposed. Since the assignment of the Dirichlet boundary conditions for displacement in this problem, is difficult, an equivalent piezo actuator deformation scheme using the Neumann boun

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Peshin, Snehan, Derosh George, Roya Shiri, Lawrence Kulinsky, and Marc Madou. "Capillary Flow-Driven and Magnetically Actuated Multi-Use Wax Valves for Controlled Sealing and Releasing of Fluids on Centrifugal Microfluidic Platforms." Micromachines 13, no. 2 (2022): 303. http://dx.doi.org/10.3390/mi13020303.

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Compact disc (CD)-based centrifugal microfluidics is an increasingly popular choice for academic and commercial applications as it enables a portable platform for biological and chemical assays. By rationally designing microfluidic conduits and programming the disc’s rotational speeds and accelerations, one can reliably control propulsion, metering, and valving operations. Valves that either stop fluid flow or allow it to proceed are critical components of a CD platform. Among the valves on a CD, wax valves that liquify at elevated temperatures to open channels and that solidify at room temper

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Neto, Estrela, Cecília J. Alves, Daniela M. Sousa, et al. "Sensory neurons and osteoblasts: close partners in a microfluidic platform." Integr. Biol. 6, no. 6 (2014): 586–95. http://dx.doi.org/10.1039/c4ib00035h.

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Harink, Björn, Séverine Le Gac, David Barata, Clemens van Blitterswijk, and Pamela Habibovic. "Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices." Lab Chip 14, no. 11 (2014): 1816–20. http://dx.doi.org/10.1039/c4lc00190g.

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Tonooka, Taishi. "Microfluidic Device with an Integrated Freeze-Dried Cell-Free Protein Synthesis System for Small-Volume Biosensing." Micromachines 12, no. 1 (2020): 27. http://dx.doi.org/10.3390/mi12010027.

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Microfluidic devices enable the precise operation of liquid samples in small volumes. This motivates why microfluidic devices have been applied to point-of-care (PoC) liquid biopsy. Among PoC liquid biopsy studies, some report diagnostic reagents being freeze-dried in such microfluidic devices. This type of PoC microfluidic device has distinct advantages, such as simplicity of the procedures, compared with other PoC devices using liquid-type diagnostic reagents. Despite the attractive characteristic, only diagnostic reagents based on the cloned enzyme donor immunoassay (CEDIA) have been freeze

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Kucukal, Erdem, Anton Ilich, Nigel S. Key, Jane A. Little, and Umut A. Gurkan. "Adhesion of Sickle RBCs to Heme-Activated Endothelial Cells Correlates with Patient Clinical Phenotypes." Blood 130, Suppl_1 (2017): 959. http://dx.doi.org/10.1182/blood.v130.suppl_1.959.959.

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Abstract Severe hemolysis and associated high levels of hemolytic biomarkers, including LDH and heme, are among major constituents of the pathophysiology of sickle cell disease (SCD). Elevated extracellular heme due to hemolysis overwhelms endogenous detoxification mechanisms and leads to oxidative stress, triggering systemic endothelium activation, vascular dysfunction, and end organ damage. To understand the role of red blood cells (RBC) in this process, we assessed sickle RBC adhesion to heme-activated endothelial cells utilizing an endothelialized microfluidic platform in a clinically dive

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Zizzari, Alessandra, and Valentina Arima. "Glass Microdroplet Generator for Lipid-Based Double Emulsion Production." Micromachines 15, no. 4 (2024): 500. http://dx.doi.org/10.3390/mi15040500.

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Microfluidics offers a highly controlled and reproducible route to synthesize lipid vesicles. In recent years, several microfluidic approaches have been introduced for this purpose, but double emulsions, such as Water-in-Oil-in-Water (W/O/W) droplets, are preferable to produce giant vesicles that are able to maximize material encapsulation. Flow focusing (FF) is a technique used to generate double emulsion droplets with high monodispersity, a controllable size, and good robustness. Many researchers use polydimethylsiloxane as a substrate material to fabricate microdroplet generators, but it ha

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Kimura, Hiroshi, Masaki Nishikawa, Takatoki Yamamoto, Yasuyuki Sakai, and Teruo Fujii. "Microfluidic Perfusion Culture of Human Hepatocytes." Journal of Robotics and Mechatronics 19, no. 5 (2007): 550–56. http://dx.doi.org/10.20965/jrm.2007.p0550.

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Analysis using human cells has been widely used in place of animal experiments. To obtain culture environments closer to those with in vivo, perfusion culture using microfluidic devices is being studied instead of stationary culture such as in a culture dish. With conventional perfusion culture with microfluidic devices, pumping system is externally provided, causing a large dead volume of culture medium. As a result, applied drugs as well as metabolites and signal transmitters from cells are diluted. We minimized this dead volume by embedding micropumps within the device to realize a high con

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