Relevant bibliographies by topics / Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)

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  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)'

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Published: 5 June 2025
Last updated: 24 June 2025

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Journal articles on the topic "Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)"

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Gong, Yan, Siyu Tian, Yang Xuan, and Shubiao Zhang. "Lipid and polymer mediated CRISPR/Cas9 gene editing." Journal of Materials Chemistry B 8, no. 20 (2020): 4369–86. http://dx.doi.org/10.1039/d0tb00207k.

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Song, Jayeon, Soohyun Kim, Hyo Yong Kim, Kyung Hoon Hur, Yoosik Kim, and Hyun Gyu Park. "A novel method to detect mutation in DNA by utilizing exponential amplification reaction triggered by the CRISPR-Cas9 system." Nanoscale 13, no. 15 (2021): 7193–201. http://dx.doi.org/10.1039/d1nr00438g.

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We, herein, describe a novel method to detect mutation in DNA by utilizing exponential amplification reaction (EXPAR) triggered by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, called CRISPR–EXPAR.
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Vaishnav, Radhika A. "The emerging role of CRISPR-Cas9 in molecular oncology." International Journal of Molecular and Immuno Oncology 2, no. 2 (2017): 45. http://dx.doi.org/10.18203/issn.2456-3994.intjmolimmunooncol20172641.

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It is not uncommon to be curious about the recent hype surrounding the new gene editing player, Cas9, which recognizes and holds into place DNA segments known as clustered regularly interspaced short palindromic repeats (CRISPR). Together, they are known as CRISPR-Cas9 or simply “CRISPR” for brevity. The binding of Cas9 causes the CRISPR sequences to become available for editing.
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Hernández-Sánchez, María. "CRISPR/Cas9 in Chronic Lymphocytic Leukemia." Encyclopedia 2, no. 2 (2022): 928–36. http://dx.doi.org/10.3390/encyclopedia2020061.

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Genome-editing systems such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology have uncovered new opportunities to model diseases such as chronic lymphocytic leukemia. CRISPR/Cas9 is an important means of advancing functional studies of Chronic Lymphocytic Leukemia (CLL) through the incorporation, elimination and modification of somatic mutations in CLL models.
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Sunusi, M., Lurwanu, Y., Halidu, J., and Musa, H. "Crispr Cas System in Plant Genome Editing a New Opportunity in Agriculture to Boost Crop Yield." UMYU Journal of Microbiology Research (UJMR) 3, no. 1 (2018): 104–14. http://dx.doi.org/10.47430/ujmr.1831.017.

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Clustered regularly interspaced short palindromic repeats CRISPR/Cas9 technology evolved from a type II bacterial immune system develop in 2013 This system employs RNA-guided nuclease, CRISPR associated (Cas9) to induce double-strand breaks. The Cas9-mediated breaks are repaired by cellular DNA repair mechanisms and mediate gene/genome modifications. The system has the ability to detect specific sequences of letters within the genetic code and to cut DNA at a specific point. Simultaneously with other sequence-specific nucleases, CRISPR/ Cas9 have already breach the boundaries and made genetic engineering much more versatile, efficient and easy also it has been reported to have increased rice grain yield up to 25-30 %, and increased tomato fruits size, branching architecture, and overall plant shape. CRISPR/ Cas also mediated virus resistance in many agricultural crops. In this article, we reviewed the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also described the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement.
 Abbreviations: CRISPR, clustered regularly interspaced short palindromic repeats; Cas, CRISPR associated; crRNA, CRISPR RNA; tracrRNA, trans-activating crRNA; PAM, protospacer adjacent motif; sgRNA, single guide RNA; gRNA, guide RNA; ssODN, single-stranded DNA oligonucleotide; DSB, double-strand break; NHEJ, non-homologous end joining; HDR, homology directed repair, CRISPRi ,CRISPR interference
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KLYMENKO, Ivan, and Mariia HALADZA. "GENOME EDITING: CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT (CRISPR/CAS9)." Modern medicine, pharmacy and psychological health, no. 3 (2023): 11–17. http://dx.doi.org/10.32689/2663-0672-2023-3-2.

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Nagarajan, Archana. "Advances in the CRISPR-Cas9 system and gene therapy." Medical Writing 32, no. 4 (2023): 46–49. http://dx.doi.org/10.56012/lzwz4471.

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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 is a genome editing tool that helps scientists modify the DNA of living organisms selectively and precisely. The discovery of this system has led to changes in the approaches to gene therapy. In this article, I delve into the role of CRISPR-Cas9 in the development of treatment using gene therapy and the drawbacks of this system. Also, I discuss the role of medical writers in the dissemination of information and research on CRISPR-Cas9 and gene therapy.
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Park, Hanseul, Jaein Shin, Hwan Choi, Byounggook Cho, and Jongpil Kim. "Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing." Cells 9, no. 6 (2020): 1447. http://dx.doi.org/10.3390/cells9061447.

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The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems.
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Tyumentseva, Marina, Aleksandr Tyumentsev, and Vasiliy Akimkin. "CRISPR/Cas9 Landscape: Current State and Future Perspectives." International Journal of Molecular Sciences 24, no. 22 (2023): 16077. http://dx.doi.org/10.3390/ijms242216077.

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CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a unique genome editing tool that can be easily used in a wide range of applications, including functional genomics, transcriptomics, epigenetics, biotechnology, plant engineering, livestock breeding, gene therapy, diagnostics, and so on. This review is focused on the current CRISPR/Cas9 landscape, e.g., on Cas9 variants with improved properties, on Cas9-derived and fusion proteins, on Cas9 delivery methods, on pre-existing immunity against CRISPR/Cas9 proteins, anti-CRISPR proteins, and their possible roles in CRISPR/Cas9 function improvement. Moreover, this review presents a detailed outline of CRISPR/Cas9-based diagnostics and therapeutic approaches. Finally, the review addresses the future expansion of genome editors’ toolbox with Cas9 orthologs and other CRISPR/Cas proteins.
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Kushwaha, Avinashi Lal, Harshit Kumar Sharma, and Chitralekha Nag Dasgupta. "The Implication and Advancement of CRISPR Genome Editing in Microalgae and Cyanobacteria." International Journal on Algae 27, no. 1 (2025): 31–50. https://doi.org/10.1615/interjalgae.v27.i1.30.

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This review paper provides an overview of recent achievements and future prospects of CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats-Cas9) genome editing in microalgae and cyanobacteria. The different types of CRISPR systems and Plasmid-based approaches, RNP-based Cas9 expression, transient and stable expression of Cas9 and sgRNA, and various other techniques for targeted gene editing have been reviewed. The paper also highlights the achievements of CRISPR-Cas9 genome editing in different cyanobacteria and algae species. Additionally, the challenges of off-target effects and potential solutions have been discussed. The paper concludes future prospects of CRISPR-Cas9 genome editing in microalgae and cyanobacteria, including gene stacking, markerless genome editing, and curing of episomes.
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Dissertations / Theses on the topic "Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)"

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Lindvall, Jenny. "Green and red fluorescent protein tagging of endogenous proteins in glioblastoma using the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 system." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-314151.

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Glioblastoma multiforme is the most malignant primary brain tumor that affects adults, recognized by the World Health Organization as an aggressive grade IV astrocytoma. Patients diagnosed with this type of tumor are left with a poor prognosis even with the most advanced treatment available. The cancer is quite heterogeneous and is typically categorized into four different subtypes depending on genetic aberrations and patient characteristics. Furthermore, researchers have discovered a subpopulation of glioblastoma cells, known as cancer stem cells, which are thought to be resistant to current therapies and responsible for tumor reoccurrence and relapse. Previous studies, in addition to this one, have found that the differentiation of glioblastoma cells downregulate nestin protein expression, the selected stem cell marker, and upregulate glial fibrillary acid protein expression, the selected differentiation marker, using immunofluorescence. Thus, one alternative treatment option is to understand the mechanism underlying the differentiation of cancer stem cells. Four cell cultures representative of each glioblastoma subtype will be endogenously tagged using the genome editing system, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9). The representative stem cell marker, nestin, will be tagged with a green fluorescent protein, while the chosen differentiation marker, glial fibrillary acid protein, will be tagged with a red fluorescent protein. Several drugs were screened to analyze whether the drugs had a differentiation effect on the glioblastoma cells. As a result, strong evidence indicated that bone morphogenetic protein four upregulated glial fibrillary acid protein expression levels to the same extent as the differentiation control media using 5% fetal bovine serum. The goal of this study is to establish a method to directly monitor the differentiation process of glioblastoma cells as a novel molecular screening method. In this case, all glioblastoma cells, even the ones resistant to treatment, can be eliminated through an initial “pre-treatment” by forcing differentiation of cancer stem cells, making the cells more susceptible to the chemotherapy drugs. In the long run, glioblastoma patients would have a chance at a more positive prognosis; a longer life that is free of glioblastoma.<br>Master Thesis in Applied Biotechnology
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Book chapters on the topic "Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)"

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Sareen, Sindhu, Pawan Saini, Charan Singh, Pradeep Kumar, and Sonia Sheoran. "Genomics and molecular physiology for improvement of drought tolerance in wheat." In Molecular breeding in wheat, maize and sorghum: strategies for improving abiotic stress tolerance and yield. CABI, 2021. http://dx.doi.org/10.1079/9781789245431.0004.

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Abstract This chapter discusses the complexity of drought tolerance in wheat focusing the morphological, biochemical, physiological and molecular responses. The breeding approaches, such as traditional and genomics-assisted strategies, for drought tolerance in wheat are described. Future perspectives are also mentioned. Before wheat genome sequencing, it was very difficult to dissect drought tolerance genomic regions because of large genome size and repetitive sequences. But with the availability of sequencing approaches, a large number of genomic resources has become available which extend the scope of utilization of advanced genomics approaches such as GWAM and GS, MutMap+, etc. A new genome editing approach, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated protein 9 (Cas9) system, can also be utilized for enhancement of drought tolerance in wheat. Therefore, integration of genomic approaches with precise phenotyping is the need of the hour for improving drought tolerance in wheat.
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Rasool, Lubna, Hamid Manzoor, Maria Rasul, and Abdur Rauf. "Structure and Mechanism of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) CRISPR-associated Nuclease 9 (Cas9)-mediated Genome Editing in Plants." In Genome Editing for Crop Improvement. CABI, 2025. https://doi.org/10.1079/9781800622517.0005.

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Mote, Gopika K., Pravin V. Jadhav, Arpita Mahobia, Mangesh P. Moharil, and Vilas Kharche. "GENOME ENGINEERING WITH CRISPR/CAS9 FOR CROP IMPROVEMENT." In Futuristic Trends in Biotechnology Volume 3 Book 7. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bdbt7p1ch3.

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Targeted genome engineering, commonly referred to as genome editing, a recent development in the life sciences, is one of the best instances of a technology used to investigate the biological phenomenon. Over a decade ago, among the existing genome editing technologies like Zinc Finger Nucleases (ZFNs) and TAL effector nucleases (TALENs), the CRISPR/Cas9. (Clustered regularly interspaced short palindromic repeats/CRISPR-associated) have gain importance due to its simplicity, accessibility, low cost and flexibility. This chapter is addressing details about genome engineering with the use of CRISPR/Cas9 in crop improvement. It also glimpse on the history of genome engineering, variants of CRISPR/Cas system, components of CRISPR/Cas system, advances in CRISPR/cas technology like base editing, prime editing, CRISPR multiplexing, epigenome editing, challenges in use of CRISPR/Cas9.
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Kefale, Habtamu, and Sewnet Getahun. "Gene Editing Improves the Agronomic Important Traits of Wheat. CRISPR-Cas9 and Cas12/Cpf1." In Wheat [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.103867.

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A hexaploid Wheat (Triticum aestivum L.) is the 3rd most important staple food crop with 15% caloric intake next to maize and rice in the world. The global attention for wheat improvement are still encouraging. However, the population growth and demand for food at this time and in the next years could not be balanced. Due to this, huge investments have been established and performed to improve the most important agronomic traits of wheat. Among the new molecular tools and techniques that have given a big emphasis as it will have many concerns is gene editing. Many gene editing tools have been reported and being implemented including Zinc finger nuclease, transcription activator-like effector nuclease, and clustered regularly interspaced short palindromic repeats associated Cas9/12 system for targeted gene editing. Among these, clustered regularly interspaced short palindromic repeats associated Cas9/12 systems are very accurate and widely used for targeted gene editing. By using CRISPR-Cas mediated gene editing technique, important traits of wheat include disease and pest resistance, better grain and flour quality, gluten-free trait, better nutritional value, nitrogen use efficiency, threshability, and other yield components and has been edited and improved. Therefore, the use of gene editing technologies for wheat as well as other important crops improvement was irreversible.
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S. Dev, Sona, Jini Joseph, and Ligi Lambert D’Rosario. "Prospects for Genome Editing of Potato." In Solanum tuberosum - a Promising Crop for Starvation Problem [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99278.

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Potato (Solanum tuberosum L.) is a staple food crop that could play a major role in improving food security in developing nations. The sustainable production of this crop faces many challenges like pests, diseases, abiotic stresses and post-harvest problems. Transgenic technology and gene silencing strategies offered a new hope of solution to the conventional time consuming breeding programmes. However the genetically modified crops are affected by regulatory approvals and safety concerns. In this aspect, gene editing techniques like ZFNs (zinc-finger nucleases), TALENs (transcription activator-like effector nucleases), and CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated Cas9), offer better choice for production of transgene and marker free disease resistant potatoes.
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Pande, Kaumudi, PP Mubthasima, Rajalakshmi Prakash, and Anbarasu Kannan. "Exosomal Delivery of CRISPR/CAS9 Assembly: Approach towards Cancer Therapeutics." In Promising Cancer Therapeutic Drug Targets: Recent Advancements. BENTHAM SCIENCE PUBLISHERS, 2025. https://doi.org/10.2174/9789815238570125010003.

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Exorbitant cancer malignancy is at the helm of multiple organ malfunction in humans and is considered a cause of increased cancer mortality worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) are powerful machinery for the therapeutic approach to tumors because of their substantial peculiarity, focusing on modulatory molecules, both oncogenes and tumor suppressors, to preclude tumor metastasis and enable apoptosis. Exosomes are considered an ideal delivery system because of their specificity and ability to prevent premature release of cargo. Exosomes are accessed as an effective conveyance of CRISPR/Cas9 elements and other attractive biomolecules to recipient cancer cells. The CRISPR/Cas9 loaded exosomes are endocytosed for further alteration of cellular metabolic pathways, either by knock-in or knock-out of the designed destined gene using sgRNA and Cas9 protein. The current study provides a platform to address the alliance between the CRISPR/Cas9 model and exosomes, depicting a remarkable therapeutic approach against cancer and other fatal diseases.
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Cassidy, Annelise, and Stephane Pelletier. "Emerging CRISPR Technologies." In CRISPR Technology [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.106652.

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The discovery and implementation of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas) systems for genome editing has revolutionized biomedical research and holds great promise for the treatment of human genetic disorders. In addition to the popular CRISPR-Cas9 and CRISPR-Cpf1 systems for genome editing, several additional Class I and Class 2 CRISPR-Cas effectors have been identified and adapted for genome editing and transcriptome modulation. Here we discuss current and emerging CRISPR-based technologies such as Cascade-Cas3, CRISPR-associated transposases (CAST), CRISPR-Cas7–11, and CRISPR-Cas13 for genome and transcriptome modification. These technologies allow for the removal or insertion of large DNA elements, the modulation of gene expression at the transcriptional level, and the editing of RNA transcripts, expanding the capabilities of current technologies.
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Upadhyay, Isha, and Ankita Singh. "UTILISING CRISPR/Cas 9 FOR GENE EDITING TO ADDRESS HEREDITARY MOVEMENT DISORDER." In Futuristic Trends in Biotechnology Volume 3 Book 9. Iterative International Publisher, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bbbt9p2ch5.

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Gene therapy offers a promising approach for addressing hereditary movement disorders like Huntington's disease, Ataxia, Dystonia and Parkinson's disease. A method known as genome editing, involves the modification of DNA by inserting, deleting, or replacing specific sequences, has gained much attention in the past few years. A prominent tool in this field is the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) system. Cas9 is obtained from the bacteria, Campylobacter jejuni. CRISPR-Cas was originally a part of the Streptococcus pyogenes adaptive immune system, but CRISPR/Cas9 now serves as a valuable tool for genome editing, causing breaks in the DNA strands under the guidance of RNA (gRNA). In contrast to alternative genome editing techniques like zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), CRISPR/Cas9 stands out for its potential clinical applicability due to its convenient in vivo delivery. It is highly specific and cuts only a limited number of sites in the genome. This chapter explores and assesses the viability of CRISPR/Cas9 in the preclinical research and its potential use in gene therapy for hereditary movement disorders.
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Ambrin, Ghazala, and Rashke Eram. "Perspective Chapter: Major Insights into CRISPR-Cas9 in Edible Oilseeds Research." In Edible Oilseeds Research - Updates and Prospects [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.114967.

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Edible oilseeds significantly contribute to human nutrition and health. However, the production and consumption of edible oilseeds are facing several challenges, such as limited land and water resources, stress factors, and the quality of edible oils. Owing to its precision and versatility, the technology of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 has emerged as a vital tool in the field of oilseed research. CRISPR-Cas9 simplifies the process, allowing scientists to tailor oilseed crops more precisely for industrial applications, nutritional purposes, yield and quality, and stress tolerance. In particular, this technology is playing a crucial role in modifying the fatty acid composition in oilseed crops, addressing industry demands, and is eventually promoting sustainable agriculture. Interestingly, the focus on increasing fatty acid composition is significant for meeting the diverse needs of both industries and consumers. Taking into account relevant literature, this chapter overviews CRISPR-Cas9 system, discusses the major insights into recent applications and achievements of CRISPR-Cas9 in edible oilseed research, addresses the major challenges and proposing solutions for CRISPR/Cas9 editing applications in edible oilseed research, and suggests the themes, so far least explored in the current context.
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Holland, Barbara Jane. "Gene Editing Technology and Ethical Issues." In Encyclopedia of Information Science and Technology, Fifth Edition. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-3479-3.ch136.

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CRISPR (clustered regularly interspaced short palindromic repeats) technology has emerged as a powerful technology for genome editing and is now widely used in basic biomedical research to explore gene function. More recently, this technology has been increasingly applied to the study or treatment of human diseases. CRISPR/Cas9 gene editing has also been applied in immunology-focused applications such as the targeting of C-C chemokine receptor type 5, the programmed death 1 gene and the creation of chimeric antigen receptors in T cells for purposes such as the treatment of the acquired immune deficiency syndrome (AIDS) or promoting anti-tumor immunotherapy. Furthermore, scientist recently suggest through their study that CRISPR may not work for everyone. This paper will review gene editing technology and its ethical concerns.
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Conference papers on the topic "Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)"

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Sá, Beatriz Lima Rodrigues de, Beatriz Pires De Oliveira Vinhas Cruz, João Pedro Souza Da Silva, Rafael Leite Carvalho, and Vinicius Canato Santana. "UTILIZAÇÃO DA TECNOLOGIA MOLECULAR CRISPR NA TERAPIA CONTRA O CÂNCER (REVISÃO SISTEMÁTICA)." In II Congresso Brasileiro de Biologia Molecular On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/2332.

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Introdução: Com a crescente ampliação do progresso tecnológico genético e molecular foram abertas inúmeras fronteiras para o entendimento e realização de modificações diretamente no genoma humano, possibilitando a compreensão de doenças que acometem pessoas atualmente, sendo elas, em primeiro lugar, o câncer; ocasionado através de alterações no DNA. Ocupa o segundo lugar no ranking de doenças que mais matam no mundo, no Brasil ultrapassa os 200 mil óbitos. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) é uma técnica de edição de DNA criada porJennifer Doudna e Emmanuelle Charpentier no ano de 2012, elas iniciaram a pesquisa para desvendar o ataque das bactérias e as infecções virais e descobriram um sistema imunológico que detecta o DNA viral e o elimina. Foi descoberto também a proteína Cas9, sendo ela capaz de clivar e degradar o DNA viral, provando ser possível usá-la para edição de genomas. Logo, CRISPR é a mais nova tecnologia da biologia molecular para possíveis curas de doenças genéticas. Ela permite que seja realizado “cortes” em trechos do DNA, substituindo por outra desejada, ocasionando modificação do gene. Objetivos: Explorar as diversas técnicas do sistema CRISPR-CAS9 ao combate de células cancerígenas, sua atuação no sistema imune reunindo diversos estudos atuais em uma só revisão, comparar metodologias usadas e seus respectivos resultados e estudar a fundo os mecanismos que possibilitam seu funcionamento. Metodologia: Análise de artigos presentes nos bancos de dados como PubMed, MEDLINE e LILACS através da busca por palavras-chave padronizadas na seguinte configuração: "Crispr AND treatment AND cancer" alternando as lacunas e escritas em inglês. Resultados: Estudos in vivo e in vitro pulicados em 2018 detalharam todo o funcionamento da CRISPR, classificada pelos pesquisadores como uma técnica promissora, viável, rápida e barata. Contudo, os estudos atuais focalizam vetores seguros para métodos de entrega da técnica em células humanas. Conclusão: Por se tratar de uma técnica nova e prática, é necessário mais estudos à fim de avaliar sua segurança e total eficácia, no entanto, outra questão importante é o fato da técnica envolver conceitos éticos e morais visto que pode ser usada para determinar ou alterar características em embriões humanos.
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Reports on the topic "Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR-Cas9) andvirus (short repeat Cas9)"

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Gong, Ping. Invasive species management on military lands : clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9)-based gene drives. Environmental Laboratory (U.S.), 2017. http://dx.doi.org/10.21079/11681/22721.

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