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1

Wu, Ren-Huang, Ming-Han Tsai, Day-Yu Chao, and Andrew Yueh. "Scanning Mutagenesis Studies Reveal a Potential Intramolecular Interaction within the C-Terminal Half of Dengue Virus NS2A Involved in Viral RNA Replication and Virus Assembly and Secretion." Journal of Virology 89, no. 8 (February 4, 2015): 4281–95. http://dx.doi.org/10.1128/jvi.03011-14.

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ABSTRACTThe NS2A protein of dengue virus (DENV) has eight predicted transmembrane segments (pTMSs; pTMS1 to pTMS8). NS2A has been shown to participate in RNA replication, virion assembly, and the host antiviral response. However, the role of the amino acid residues within the pTMS regions of NS2A during the virus life cycle is poorly understood. In the study described here, we explored the function of DENV NS2A by introducing a series of double or triple alanine substitutions into the C-terminal half (pTMS4 to pTMS8) of NS2A in the context of a DENV infectious clone or subgenomic replicon. Fourteen (8 within pTMS8) of 35 NS2A mutants displayed a lethal phenotype due to impairment of RNA replication by a replicon assay. Three NS2A mutants with mutations within pTMS7, the CM20, CM25, and CM27 mutants, displayed similar phenotypes, low virus yields (>100-fold reduction), wild-type-like replicon activity, and low infectious virus-like particle yields by transienttrans-packaging experiments, suggesting a defect in virus assembly and secretion. The sequencing of revertant viruses derived from CM20, CM25, and CM27 mutant viruses revealed a consensus reversion mutation, leucine (L) to phenylalanine (F), at codon 181 within pTMS7. The introduction of an L181F mutation into a full-length NS2A mutant, i.e., the CM20, CM25, and CM27 constructs, completely restored wild-type infectivity. Notably, L181F also substantially rescued the other severely RNA replication-defective mutants with mutations within pTMS4, pTMS6, and pTMS8, i.e., the CM2, CM3, CM13, CM31, and CM32 mutants. In conclusion, the results revealed the essential roles of pTMS4 to pTMS8 of NS2A in RNA replication and/or virus assembly and secretion. The intramolecular interaction between pTMS7 and pTMS4, pTMS6, or pTMS8 of the NS2A protein was also implicated.IMPORTANCEThe reported characterization of the C-terminal half of dengue virus NS2A is the first comprehensive mutagenesis study to investigate the function of flavivirus NS2A involved in the steps of the virus life cycle. In particular, detailed mapping of the amino acid residues within the predicted transmembrane segments (pTMSs) of NS2A involved in RNA replication and/or virus assembly and secretion was performed. A revertant genetics study also revealed that L181F within pTMS7 is a consensus reversion mutation that rescues both RNA replication-defective and virus assembly- and secretion-defective mutants with mutations within the other three pTMSs of NS2A. Collectively, these findings elucidate the role played by NS2A during the virus life cycle, possibly through the intricate intramolecular interaction between pTMS7 and other pTMSs within the NS2A protein.
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2

Griffies, Stephen M., Michael Winton, Whit G. Anderson, Rusty Benson, Thomas L. Delworth, Carolina O. Dufour, John P. Dunne, et al. "Impacts on Ocean Heat from Transient Mesoscale Eddies in a Hierarchy of Climate Models." Journal of Climate 28, no. 3 (February 1, 2015): 952–77. http://dx.doi.org/10.1175/jcli-d-14-00353.1.

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Abstract The authors characterize impacts on heat in the ocean climate system from transient ocean mesoscale eddies. Their tool is a suite of centennial-scale 1990 radiatively forced numerical climate simulations from three GFDL coupled models comprising the Climate Model, version 2.0–Ocean (CM2-O), model suite. CM2-O models differ in their ocean resolution: CM2.6 uses a 0.1° ocean grid, CM2.5 uses an intermediate grid with 0.25° spacing, and CM2-1deg uses a nominal 1.0° grid. Analysis of the ocean heat budget reveals that mesoscale eddies act to transport heat upward in a manner that partially compensates (or offsets) for the downward heat transport from the time-mean currents. Stronger vertical eddy heat transport in CM2.6 relative to CM2.5 accounts for the significantly smaller temperature drift in CM2.6. The mesoscale eddy parameterization used in CM2-1deg also imparts an upward heat transport, yet it differs systematically from that found in CM2.6. This analysis points to the fundamental role that ocean mesoscale features play in transient ocean heat uptake. In general, the more accurate simulation found in CM2.6 provides an argument for either including a rich representation of the ocean mesoscale in model simulations of the mean and transient climate or for employing parameterizations that faithfully reflect the role of eddies in both lateral and vertical heat transport.
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3

R, Vijayaragavan. "A Complex Variable Method for Secondary Flow in a Slowly Rotating Straight Elliptic Pipe." Journal of Computational Mathematica 1, no. 2 (December 30, 2017): 54–71. http://dx.doi.org/10.26524/cm16.

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4

R, Murali, and Vithya V. "Stability of Generalized Cubic Functional Equation in Matrix Normed Spaces." Journal of Computational Mathematica 1, no. 2 (December 30, 2017): 113–20. http://dx.doi.org/10.26524/cm20.

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D, Ramesh Kumar. "Generalized Rational Inequalities in Complex Valued Metric Spaces." Journal of Computational Mathematica 1, no. 2 (December 30, 2017): 121–32. http://dx.doi.org/10.26524/cm21.

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Pinelas, Sandra, and Rajiniganth P. "Fibonacci Sequence and its Sum by Second Order Difference Operator with Polynomial Factorial." Journal of Computational Mathematica 2, no. 2 (December 30, 2018): 20–29. http://dx.doi.org/10.26524/cm36.

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7

J, Ravi, Dickson S, and Sathya K. "An Optimal Solution for Transportation problem-DFSD." Journal of Computational Mathematica 3, no. 1 (June 30, 2019): 40–48. http://dx.doi.org/10.26524/cm46.

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8

G, Thangaraj, Vivakanandan P, and Chandiran V. "A Short Note on Pairwise Fuzzy Irresolvable Spaces." Journal of Computational Mathematica 3, no. 2 (December 30, 2019): 76–84. http://dx.doi.org/10.26524/cm56.

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9

D, Ajay, Annamalai D, and Joseline Charisma J. "Pythagorean fuzzy semi open sets." Journal of Computational Mathematica 5, no. 1 (June 18, 2021): 97–104. http://dx.doi.org/10.26524/cm96.

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Our main aim is to define the concept of semi open sets in Pythagorean topological spaces which is one of the weakest forms of open sets. Along with their introduction their characterizations and properties have been investigated with examples. In addition to that the continuous functions have also been defined.
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10

Suyraksa, Sumuncharee, Pitiya Kamonpatana, Noppadon Kerddonfag, Amporn Sane, and Vanee Chonhenchob. "Development of Conductive Packaging for Beverage Processing by Ohmic Heating." Key Engineering Materials 861 (September 2020): 213–17. http://dx.doi.org/10.4028/www.scientific.net/kem.861.213.

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This study was aimed to develop conductive packaging for ohmic heating. Polypropylene (PP) was mixed with conductive material (CM) in the ratios of 70:30 (CM30), 75:25 (CM25), and 80:20 (CM20) (w/w), then the conductive bottles were developed using extrusion blow molding process. The bottles were suspended in different sodium sulfate (Na2SO4) solutions (0.2, 0.3, and 0.5% w/w) as a transmitting current medium for ohmic heating and heated for 8 min. The CM30 and CM 25 had the highest electrical conductivity compared to the CM20, however the CM20 exhibited best processability, hence it was selected to be used for ohmic heating of orange juice. Different concentrations of Na2SO4 solutions had the effects on ohmic heating. The CM20 bottle suspended in 0.2% Na2SO4 solution resulted in the most uniform heating and suitable for ohmic processing of orange juice. The new conductive bottles developed could potentially be used for beverage processing by ohmic heating.
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11

SK, Vaidya, and Rakhimol V Isaac. "Equitable Coloring of Some Cycle Related Graphs." Journal of Computational Mathematica 1, no. 1 (June 30, 2017): 31–36. http://dx.doi.org/10.26524/cm2.

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12

Zhu, Linli, Yu Pan, Mohammad Reza Farahani, and Wei Gao. "Graph Laplacian Based Ontology Regularization Distance Framework for Ontology Similarity Measuring and Ontology Mapping." Journal of Computational Mathematica 1, no. 1 (June 30, 2017): 88–98. http://dx.doi.org/10.26524/cm6.

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13

Maier-Komor, P., and K. H. Speidel. "Sandwich targets of 0.5mg/cm2 C with 4mg/cm2 ferromagnetic Gd on 3mg/cm2 Ta support with 3.5mg/cm2 Cu backing." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 480, no. 1 (March 2002): 71–74. http://dx.doi.org/10.1016/s0168-9002(01)02050-2.

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14

G Jeni Christi A, Heri Purwoto,. "OPTIMASI FORMULA EDIBLE FILM BERBASIS AMILOPEKTIN PATI SINGKONG DAN KARAGENAN." Majalah Ilmiah Pengkajian Industri 11, no. 1 (April 15, 2017): 31–40. http://dx.doi.org/10.29122/mipi.v11i1.2091.

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Penelitian ini dilakukan untuk mendapatkan formula optimum antara amilopektin pati singkong dan karagenan sebagai bahan baku edible film. Program Design Expert 7.0.0 (trial version) dengan Response Surface Methodology (RSM) Central Composite Design (CCD) digunakan untuk mengoptimasi formula edible film. Variabel bebas yang digunakan yaitu persentase amilopektin, karagenan dan gliserin. Variabel respon yang diukur dan dioptimasi yaitu laju transmisi uap air, kuat tarik dan modulus elastisitas. Hasil optimasi dari program Design Expert 7.0.0 merekomendasikan 15 solusi optimasi dengan nilai desirability tertinggi sebesar 0.821. Formula satu dipilih untuk divalidasi dengan faktor-faktor yaitu amilopektin 3.00 %, karagenan 2.00 %, dan gliserin 2.00 %. Nilai respon prediksi untuk laju transmisi uap air sebesar 16.331 g/m2/24 jam, kuat tarik sebesar 180.657 kgf/cm2 dan modulus elastisitas sebesar 139.262 kgf/cm2. Nilai hasil validasi untuk laju transmis uap air sebesar 16.1027 g/m2/24 jam, kuat tarik sebesar 208.42 kgf/cm2 dan modulus elastisitas sebesar 183.05 kgf/cm2, sehingga solusi yang direkomendasikan oleh program sudah cukup baik.
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15

Makanya, A. N., J. N. Maina, T. M. Mayhew, S. A. Tschanz, and P. H. Burri. "A stereological comparison of villous and microvillous surfaces in small intestines of frugivorous and entomophagous bats: species, inter-individual and craniocaudal differences." Journal of Experimental Biology 200, no. 18 (September 1, 1997): 2415–23. http://dx.doi.org/10.1242/jeb.200.18.2415.

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The extents of functional surfaces (villi, microvilli) have been estimated at different longitudinal sites, and in the entire small intestine, for three species of bats belonging to two feeding groups: insect- and fruit-eaters. In all species, surface areas and other structural quantities tended to be greatest at more cranial sites and to decline caudally. The entomophagous bat (Miniopterus inflatus) had a mean body mass (coefficient of variation) of 8.9 g (5%) and a mean intestinal length of 20 cm (6%). The surface area of the basic intestinal tube (primary mucosa) was 9.1 cm2 (10%) but this was amplified to 48 cm2 (13%) by villi and to 0.13 m2 (20%) by microvilli. The total number of microvilli per intestine was 4 x 10(11) (20%). The average microvillus had a diameter of 8 nm (10%), a length of 1.1 microns (22%) and a membrane surface area of 0.32 micron 2 (31%). In two species of fruit bats (Epomophorus wahlbergi and Lisonycteris angolensis), body masses were greater and intestines longer, the values being 76.0 g (18%) and 76.9 g (4%), and 73 cm (16%) and 72 cm (7%), respectively. Surface areas were also greater, amounting to 76 cm2 (26%) and 45 cm2 (8%) for the primary mucosa, 547 cm2 (29%) and 314 cm2 (16%) for villi and 2.7 m2 (23%) and 1.5 m2 (18%) for microvilli. An increase in the number of microvilli, 33 x 10(11) (19%) and 15 x 10(11) (24%) per intestine, contributed to the more extensive surface area but there were concomitant changes in the dimensions of microvilli. Mean diameters were 94 nm (8%) and 111 nm (4%), and mean lengths were 2.8 microns (12%) and 2.9 microns (10%), respectively. Thus, an increase in the surface area of the average microvillus to 0.83 micron 2 (12%) and 1.02 microns 2 (11%) also contributed to the greater total surface area of microvilli. The lifestyle-related differences in total microvillous surface areas persisted when structural quantities were normalised for the differences in body masses. The values for total microvillous surface area were 148 cm2g-1 (20%) in the entomophagous bat, 355 cm2g-1 (20%) in E. wahlbergi and 192 cm2g-1 (17%) in L. angolensis. This was true despite the fact that the insecteater possessed a greater length of intestine per unit of body mass: 22 mm g-1 (8%) versus 9-10 mm g-1 (9-10%) for the fruit-eaters.
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16

Beaumanoir-Secq, Morgane, and Claire Renvoisé. "L’adjectif au CM2." Le français aujourd'hui N°211, no. 4 (2020): 45. http://dx.doi.org/10.3917/lfa.211.0045.

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17

Lee, Sang Yeob, Sung Kee Hong, Jeong Jun Kim, Ji Hee Han, and Wan Gyu Kim. "Biological control of Paraconiothyrium minitans CM2 on Lettuce Sclerotinia Rot Caused by Sclerotinia sclerotiorum." Korean Journal of Mycology 40, no. 4 (December 31, 2012): 271–76. http://dx.doi.org/10.4489/kjm.2012.40.4.271.

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18

Skelly, Andrea C. "Beyond 100 mW/cm2." Journal of Diagnostic Medical Sonography 1, no. 5 (September 1985): 190–97. http://dx.doi.org/10.1177/875647938500100502.

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19

Shekhtman, L., F. Ignatov, and V. Tayursky. "Simulation of physics background in Super c-tau factory detector." EPJ Web of Conferences 212 (2019): 01009. http://dx.doi.org/10.1051/epjconf/201921201009.

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Simulation of background particle fluxes generated by colliding beams is performed with FLUKA package for the Super C-Tau factory Detector (SCTD). Two processes are considered as main sources of luminosity generated background: two-photon production of electron-positron pairs and Bha-Bha scattering with bremsstrahlung photon emission (radiative Bha-Bha). The SCTD geometry is described corresponding to the last version of the Conceptual Design Report. The magnetic field based on the calculation in ANSYS is introduced in the model. Main results of the simulation for beam energy of 3 GeV, luminosity of 1035 cm−2s−1 and 1.5 T magnetic field are the following: charged particle fluence in the region of the Inner Tracker (radius 5cm -20 cm, Z between -30cm and 30 cm) is between 105 particles/(cm2s) and ∼103 particles/(cm2s); 1-MeV neutron equivalent fluence for Si in the regions corresponding to electronics of the Inner Tracker and the Drift Chamber is below 1011 n/(cm2y) and absorbed dose is below 100 Gy/y in the hottest regions of the detector.
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20

Lee, Sang-Yeob, Wan-Gyu Kim, Sung-Kee Hong, Hang-Yeon Weon, and Kyung-Seok Park. "Inhibitory Effect of Paraconiothyrium minitans CM2 on Sclerotial Germination of Sclerotinia sclerotiorum and S. minor Causing Sclerotinia Rot of Lettuce." Korean Journal of Mycology 39, no. 2 (August 1, 2011): 131–35. http://dx.doi.org/10.4489/kjm.2010.39.2.131.

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21

Wang, Jianguo, C. Greg Jensen, and W. Ed Red. "Global Finish Machining Using CM2." Computer-Aided Design and Applications 3, no. 1-4 (January 2006): 475–84. http://dx.doi.org/10.1080/16864360.2006.10738486.

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22

Hytönen, Tuomas, and Anna Kairema. "Systems of dyadic cubes in a doubling metric space." Colloquium Mathematicum 126, no. 1 (2012): 1–33. http://dx.doi.org/10.4064/cm126-1-1.

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23

Luca, Florian, and Carl Pomerance. "Acknowledgment of priority: ``On some problems of Mąkowski–Schinzel and Erdős concerning the arithmetical functions φ and σ'' (Colloq. Math. 92 (2002), 111–130)." Colloquium Mathematicum 126, no. 1 (2012): 139. http://dx.doi.org/10.4064/cm126-1-10.

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24

Barannyk, Leonid F. "Finite groups of OTP projective representation type." Colloquium Mathematicum 126, no. 1 (2012): 35–51. http://dx.doi.org/10.4064/cm126-1-2.

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Grigoriev, Ilya, Marius Cătălin Iordan, Amos Lubin, Nathaniel Ince, and Cesar E. Silva. "On μ-compatible metrics and measurable sensitivity." Colloquium Mathematicum 126, no. 1 (2012): 53–72. http://dx.doi.org/10.4064/cm126-1-3.

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26

Dieterich, Ernst. "A general approach to finite-dimensional division algebras." Colloquium Mathematicum 126, no. 1 (2012): 73–86. http://dx.doi.org/10.4064/cm126-1-4.

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27

Dey, Santanu. "Standard commuting dilations and liftings." Colloquium Mathematicum 126, no. 1 (2012): 87–94. http://dx.doi.org/10.4064/cm126-1-5.

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Moore, Charles N., and Xiaojing Zhang. "A law of the iterated logarithm for general lacunary series." Colloquium Mathematicum 126, no. 1 (2012): 95–103. http://dx.doi.org/10.4064/cm126-1-6.

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29

Dąbrowski, Andrzej. "On the Brocard–Ramanujan problem and generalizations." Colloquium Mathematicum 126, no. 1 (2012): 105–10. http://dx.doi.org/10.4064/cm126-1-7.

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30

Zheng, Jiqiang. "On blow-up for the Hartree equation." Colloquium Mathematicum 126, no. 1 (2012): 111–24. http://dx.doi.org/10.4064/cm126-1-8.

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Castro, M. H., V. A. Menegatto, and A. P. Peron. "Integral operators generated by Mercer-like kernels on topological spaces." Colloquium Mathematicum 126, no. 1 (2012): 125–38. http://dx.doi.org/10.4064/cm126-1-9.

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32

Atreas, Nikolaos, and Antonis Bisbas. "Generalized Riesz products produced from orthonormal transforms." Colloquium Mathematicum 126, no. 2 (2012): 141–54. http://dx.doi.org/10.4064/cm126-2-1.

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Houissa, Khadija, and Mohamed Sifi. "Symmetric Bessel multipliers." Colloquium Mathematicum 126, no. 2 (2012): 155–76. http://dx.doi.org/10.4064/cm126-2-2.

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34

Anisimov, Artem. "On existence of double coset varieties." Colloquium Mathematicum 126, no. 2 (2012): 177–85. http://dx.doi.org/10.4064/cm126-2-3.

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35

Shelah, Saharon. "When a first order T has limit models." Colloquium Mathematicum 126, no. 2 (2012): 187–204. http://dx.doi.org/10.4064/cm126-2-4.

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Frej, Paulina. "Entropy of a doubly stochastic Markov operator and of its shift on the space of trajectories." Colloquium Mathematicum 126, no. 2 (2012): 205–16. http://dx.doi.org/10.4064/cm126-2-5.

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Białkowski, Jerzy, Karin Erdmann, and Andrzej Skowroński. "Deformed mesh algebras of Dynkin type Cn." Colloquium Mathematicum 126, no. 2 (2012): 217–30. http://dx.doi.org/10.4064/cm126-2-6.

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Ghenciu, Ioana, and Paul Lewis. "Completely Continuous operators." Colloquium Mathematicum 126, no. 2 (2012): 231–56. http://dx.doi.org/10.4064/cm126-2-7.

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Shi, Huaguo, Zhangjia Han, and Guiyun Chen. "Dp(3) (p ≥5) can be characterized by its order components." Colloquium Mathematicum 126, no. 2 (2012): 257–68. http://dx.doi.org/10.4064/cm126-2-8.

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40

Jleli, Mohamed. "Stability results for rotationally invariant constant mean curvature surfaces in hyperbolic space." Colloquium Mathematicum 126, no. 2 (2012): 269–80. http://dx.doi.org/10.4064/cm126-2-9.

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Matoušková, Eva, and Miroslav Zelený. "A note on intersections of non-Haar null sets." Colloquium Mathematicum 96, no. 1 (2003): 1–4. http://dx.doi.org/10.4064/cm96-1-1.

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Alkhamees, Yousef, Hanan Alolayan, and Surjeet Singh. "A representation theorem for Chain rings." Colloquium Mathematicum 96, no. 1 (2003): 103–19. http://dx.doi.org/10.4064/cm96-1-10.

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Bocian, Rafał, and Andrzej Skowroński. "Symmetric special biserial algebras of euclidean type." Colloquium Mathematicum 96, no. 1 (2003): 121–48. http://dx.doi.org/10.4064/cm96-1-11.

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Mikulski, Włodzimierz M. "The natural operators T(0,0)→T(1,1)T(r)." Colloquium Mathematicum 96, no. 1 (2003): 5–16. http://dx.doi.org/10.4064/cm96-1-2.

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Mikulski, Włodzimierz M., and Jiří M. Tomáš. "Product preserving bundle functors on fibered fibered manifolds." Colloquium Mathematicum 96, no. 1 (2003): 17–26. http://dx.doi.org/10.4064/cm96-1-3.

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46

Kawamura, Kazuhiro. "Low dimensional homotopy groups of suspensions of the Hawaiian earring." Colloquium Mathematicum 96, no. 1 (2003): 27–39. http://dx.doi.org/10.4064/cm96-1-4.

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47

Melzi, Camillo. "Sub-Laplacian with drift in nilpotent Lie groups." Colloquium Mathematicum 96, no. 1 (2003): 41–53. http://dx.doi.org/10.4064/cm96-1-5.

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48

Rittaud, Benoît. "Équidistribution presque partout modulo 1 de suites oscillantes perturbées – II : cas liouvillien unidimensionnel." Colloquium Mathematicum 96, no. 1 (2003): 55–73. http://dx.doi.org/10.4064/cm96-1-6.

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Drungilas, Paulius, and Artūras Dubickas. "Multiplicative dependence of shifted algebraic numbers." Colloquium Mathematicum 96, no. 1 (2003): 75–81. http://dx.doi.org/10.4064/cm96-1-7.

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Alzer, Horst, and Stamatis Koumandos. "A sharp bound for a sine polynomial." Colloquium Mathematicum 96, no. 1 (2003): 83–91. http://dx.doi.org/10.4064/cm96-1-8.

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