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Journal articles on the topic 'Co-translational assembly'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Wells, Jonathan N., L. Therese Bergendahl, and Joseph A. Marsh. "Co-translational assembly of protein complexes." Biochemical Society Transactions 43, no. 6 (2015): 1221–26. http://dx.doi.org/10.1042/bst20150159.

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The interaction of biological macromolecules is a fundamental attribute of cellular life. Proteins, in particular, often form stable complexes with one another. Although the importance of protein complexes is widely recognized, we still have only a very limited understanding of the mechanisms underlying their assembly within cells. In this article, we review the available evidence for one such mechanism, namely the coupling of protein complex assembly to translation at the polysome. We discuss research showing that co-translational assembly can occur in both prokaryotic and eukaryotic organism

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2

Williams, Neal K., and Bernhard Dichtl. "Co-translational control of protein complex formation: a fundamental pathway of cellular organization?" Biochemical Society Transactions 46, no. 1 (2018): 197–206. http://dx.doi.org/10.1042/bst20170451.

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Analyses of proteomes from a large number of organisms throughout the domains of life highlight the key role played by multiprotein complexes for the implementation of cellular function. While the occurrence of multiprotein assemblies is ubiquitous, the understanding of pathways that dictate the formation of quaternary structure remains enigmatic. Interestingly, there are now well-established examples of protein complexes that are assembled co-translationally in both prokaryotes and eukaryotes, and indications are that the phenomenon is widespread in cells. Here, we review complex assembly wit

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Zhang, Lixin, Virpi Paakkarinen, Klaas Jan van Wijk, and Eva-Mari Aro. "Co-translational Assembly of the D1 Protein into Photosystem II." Journal of Biological Chemistry 274, no. 23 (1999): 16062–67. http://dx.doi.org/10.1074/jbc.274.23.16062.

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4

Panasenko, Olesya O., Syam Prakash Somasekharan, Zoltan Villanyi, et al. "Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes." Nature Structural & Molecular Biology 26, no. 2 (2019): 110–20. http://dx.doi.org/10.1038/s41594-018-0179-5.

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5

Tang, Jia Xin, Kyle Thompson, Robert W. Taylor, and Monika Oláhová. "Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways." International Journal of Molecular Sciences 21, no. 11 (2020): 3820. http://dx.doi.org/10.3390/ijms21113820.

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The assembly of mitochondrial oxidative phosphorylation (OXPHOS) complexes is an intricate process, which—given their dual-genetic control—requires tight co-regulation of two evolutionarily distinct gene expression machineries. Moreover, fine-tuning protein synthesis to the nascent assembly of OXPHOS complexes requires regulatory mechanisms such as translational plasticity and translational activators that can coordinate mitochondrial translation with the import of nuclear-encoded mitochondrial proteins. The intricacy of OXPHOS complex biogenesis is further evidenced by the requirement of many

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Rutledge, Angela C., Qiaozhu Su, and Khosrow Adeli. "Apolipoprotein B100 biogenesis: a complex array of intracellular mechanisms regulating folding, stability, and lipoprotein assemblyThis paper is one of a selection of papers published in this special issue entitled “Canadian Society of Biochemistry, Molecular & Cellular Biology 52nd Annual Meeting — Protein Folding: Principles and Diseases” and has undergone the Journal's usual peer review process." Biochemistry and Cell Biology 88, no. 2 (2010): 251–67. http://dx.doi.org/10.1139/o09-168.

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Apolipoprotein B100 (apoB) is a large amphipathic lipid-binding protein that is synthesized by hepatocytes and used to assemble and stabilize very low density lipoproteins (VLDL). It may have been derived through evolution from other lipid-associating proteins such as microsomal triglyceride transfer protein or vitellogenin. The correct folding of apoB requires assistance from chaperone proteins in co-translational lipidation, disulfide bond formation, and glycosylation. Any impairment in these processes results in co-translational targeting of the misfolded apoB molecule for proteasomal degra

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Lautier, Ophélie, Arianna Penzo, Jérôme O. Rouvière, et al. "Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis." Molecular Cell 81, no. 11 (2021): 2417–27. http://dx.doi.org/10.1016/j.molcel.2021.03.030.

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8

Wagner, Susan, Anna Herrmannová, Vladislava Hronová, et al. "Selective Translation Complex Profiling Reveals Staged Initiation and Co-translational Assembly of Initiation Factor Complexes." Molecular Cell 79, no. 4 (2020): 546–60. http://dx.doi.org/10.1016/j.molcel.2020.06.004.

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9

Kassem, Sari, Zoltan Villanyi, and Martine A. Collart. "Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA." Nucleic Acids Research 45, no. 3 (2016): 1186–99. http://dx.doi.org/10.1093/nar/gkw1059.

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10

Kassem, Sari, Zoltan Villanyi, and Martine A. Collart. "Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA." Nucleic Acids Research 45, no. 12 (2017): 7539. http://dx.doi.org/10.1093/nar/gkx447.

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11

Shrimal, Shiteshu, Natalia A. Cherepanova, and Reid Gilmore. "DC2 and KCP2 mediate the interaction between the oligosaccharyltransferase and the ER translocon." Journal of Cell Biology 216, no. 11 (2017): 3625–38. http://dx.doi.org/10.1083/jcb.201702159.

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In metazoan organisms, the STT3A isoform of the oligosaccharyltransferase is localized adjacent to the protein translocation channel to catalyze co-translational N-linked glycosylation of proteins in the endoplasmic reticulum. The mechanism responsible for the interaction between the STT3A complex and the translocation channel has not been addressed. Using genetically modified human cells that are deficient in DC2 or KCP2 proteins, we show that loss of DC2 causes a defect in co-translational N-glycosylation of proteins that mimics an STT3A−/− phenotype. Biochemical analysis showed that DC2 and

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12

Toki, Naoko, Hazuki Takahashi, Harshita Sharma, et al. "SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies." Nucleic Acids Research 48, no. 20 (2020): 11626–44. http://dx.doi.org/10.1093/nar/gkaa814.

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Abstract SINEUPs are long non-coding RNAs (lncRNAs) that contain a SINE element, and which up-regulate the translation of target mRNA. They have been studied in a wide range of applications, as both biological and therapeutic tools, although the underpinning molecular mechanism is unclear. Here, we focused on the sub-cellular distribution of target mRNAs and SINEUP RNAs, performing co-transfection of expression vectors for these transcripts into human embryonic kidney cells (HEK293T/17), to investigate the network of translational regulation. The results showed that co-localization of target m

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13

Liu, Wallace H., and Mair E. A. Churchill. "Histone transfer among chaperones." Biochemical Society Transactions 40, no. 2 (2012): 357–63. http://dx.doi.org/10.1042/bst20110737.

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The eukaryotic processes of nucleosome assembly and disassembly govern chromatin dynamics, in which histones exchange in a highly regulated manner to promote genome accessibility for all DNA-dependent processes. This regulation is partly carried out by histone chaperones, which serve multifaceted roles in co-ordinating the interactions of histone proteins with modification enzymes, nucleosome remodellers, other histone chaperones and nucleosomal DNA. The molecular details of the processes by which histone chaperones promote delivery of histones among their many functional partners are still la

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14

Roy, Somdutta, and Martin Tenniswood. "Site-specific Acetylation of p53 Directs Selective Transcription Complex Assembly." Journal of Biological Chemistry 282, no. 7 (2006): 4765–71. http://dx.doi.org/10.1074/jbc.m609588200.

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Histone deacetylase (HDAC) inhibitors are being investigated as possible adjuvant therapies for a number of diseases, including cancer. In addition to stabilization of acetylated histones, HDAC inhibitors stabilize the acetylation of a number of transcription factors, including p53. This study investigates the action of two HDAC inhibitors, CG-1521 and trichostatin A, which stabilize Ac-Lys-373 p53 and Ac-Lys-382 p53, respectively, in LNCaP prostate cancer cells. Real-time PCR demonstrates that CG-1521 induces p21 transcription whereas trichostatin A does not alter the steady state level of p2

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Laird, D. W., K. Jordan, P. Fistouris, J. L. Solan, P. D. Lampe, and C. Shao. "Transport of Connexins and Gap Junction Turnover in Living Cells Visualized Using Green Fluorescent Protein Tagged Connexins." Microscopy and Microanalysis 5, S2 (1999): 1020–21. http://dx.doi.org/10.1017/s1431927600018420.

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Connexins oligomerize into hemichannels, traffic to the cell surface and dock with hemichannels from an adjacent cell to form intercellular gap junction channels. Pulsechase studies have revealed that connexins are subject to a short half-life ranging from 1- 5 hrs. To examine the mechanisms involved in connexin trafficking, gap junction assembly and internalization in living cells we fused green fluorescent protein (GFP) to the amino or carboxyl terminus of full length connexins (Cx). When cDNA encoding Cx43-GFP was transfected into communication-competent NRK cells, Cx43-negative MDCK cells,

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16

Doh, Jung H., Sheila Lutz, and M. Joan Curcio. "Co-translational Localization of an LTR-Retrotransposon RNA to the Endoplasmic Reticulum Nucleates Virus-Like Particle Assembly Sites." PLoS Genetics 10, no. 3 (2014): e1004219. http://dx.doi.org/10.1371/journal.pgen.1004219.

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17

Majeau, Nathalie, Marilène Bolduc, Jean-Baptiste Duvignaud, Rémi Fromentin, and Denis Leclerc. "Effect of cAMP-dependent protein kinase A (PKA) on HCV nucleocapsid assembly and degradation." Biochemistry and Cell Biology 85, no. 1 (2007): 78–87. http://dx.doi.org/10.1139/o06-195.

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The primary function of the hepatitis C virus (HCV) core protein is genome encapsidation. Core protein is also subject to post-translational modifications that can impact on the assembly process. In this report, we have studied the effect of cAMP-dependent protein kinase A (PKA) phosphorylation on its assembly and stability in a yeast Pichia pastoris expression system. We have recently shown that co-expression of the human signal peptide peptidase and core protein (amino acids 1–191) in yeast leads to the formation of nucleocapsid-like particles (NLPs) that are morphologically similar to the w

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18

Pardal, Alonso J., Filipe Fernandes-Duarte, and Andrew J. Bowman. "The histone chaperoning pathway: from ribosome to nucleosome." Essays in Biochemistry 63, no. 1 (2019): 29–43. http://dx.doi.org/10.1042/ebc20180055.

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AbstractNucleosomes represent the fundamental repeating unit of eukaryotic DNA, and comprise eight core histones around which DNA is wrapped in nearly two superhelical turns. Histones do not have the intrinsic ability to form nucleosomes; rather, they require an extensive repertoire of interacting proteins collectively known as ‘histone chaperones’. At a fundamental level, it is believed that histone chaperones guide the assembly of nucleosomes through preventing non-productive charge-based aggregates between the basic histones and acidic cellular components. At a broader level, histone chaper

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19

Villanyi, Zoltan, and Martine A. Collart. "Ccr4–Not is at the core of the eukaryotic gene expression circuitry." Biochemical Society Transactions 43, no. 6 (2015): 1253–58. http://dx.doi.org/10.1042/bst20150167.

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In this mini-review, we summarize our current knowledge about the cross-talk between the different levels of gene expression. We introduce the Ccr4 (carbon catabolite repressed 4)–Not (negative on TATA-less) complex as a candidate to be a master regulator that orchestrates between the different levels of gene expression. An integrated view of the findings about the Ccr4–Not complex suggests that it is involved in gene expression co-ordination. Since the discovery of the Not proteins in a selection for transcription regulators in yeast [Collart and Struhl (1994) Genes Dev. 8, 525–537], the Ccr4

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20

Lambert, Carsten, Sylvia Mann, and Reinhild Prange. "Assessment of determinants affecting the dual topology of hepadnaviral large envelope proteins." Journal of General Virology 85, no. 5 (2004): 1221–25. http://dx.doi.org/10.1099/vir.0.19737-0.

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For functional diversity, the large (L) envelope protein of hepatitis B virus (HBV) acquires a dual transmembrane topology via co-translational membrane integration of the S region and partial post-translational translocation of the preS subdomain. Because each process requires the second transmembrane segment (TM2), we explored the action of this determinant by using protease protection analysis of mutant L proteins. We demonstrated that neither the disruption of a leucine zipper-like motif by multiple alanine substitutions nor the flanking charges of TM2 affected the topological reorientatio

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21

Redick, S. D., and J. E. Schwarzbauer. "Rapid intracellular assembly of tenascin hexabrachions suggests a novel cotranslational process." Journal of Cell Science 108, no. 4 (1995): 1761–69. http://dx.doi.org/10.1242/jcs.108.4.1761.

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Tenascin, an extracellular matrix protein that modulates cell adhesion, exists as a unique six-armed structure called a hexabrachion. The human hexabrachion is composed of six identical 320 kDa subunits and the structure is stabilized by inter-subunit disulfide bonds between amino-terminal segments. We have examined the biosynthesis of tenascin and its assembly into hexabrachions using pulsechase labeling of U-138 MG human glioma cells. Newly synthesized tenascin hexamers are secreted within 60 minutes of translation initiation. Intracellularly, as early as full length tenascin can be detected

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22

Bann, D. V., A. R. Beyer, and L. J. Parent. "A Murine Retrovirus Co-Opts YB-1, a Translational Regulator and Stress Granule-Associated Protein, To Facilitate Virus Assembly." Journal of Virology 88, no. 8 (2014): 4434–50. http://dx.doi.org/10.1128/jvi.02607-13.

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23

Pariyarath, Rajalakshmi, Hongxing Wang, John D. Aitchison, et al. "Co-translational Interactions of Apoprotein B with the Ribosome and Translocon during Lipoprotein Assembly or Targeting to the Proteasome." Journal of Biological Chemistry 276, no. 1 (2000): 541–50. http://dx.doi.org/10.1074/jbc.m007944200.

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24

Rana, Jyoti, José Luis Slon Campos, Monica Poggianella, and Oscar R. Burrone. "Dengue virus capsid anchor modulates the efficiency of polyprotein processing and assembly of viral particles." Journal of General Virology 100, no. 12 (2019): 1663–73. http://dx.doi.org/10.1099/jgv.0.001346.

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The assembly and secretion of flaviviruses are part of an elegantly regulated process. During maturation, the viral polyprotein undergoes several co- and post-translational cleavages mediated by both viral and host proteases. Among these, sequential cleavage at the N and C termini of the hydrophobic capsid anchor (Ca) is crucial in deciding the fate of viral infection. Here, using a refined dengue pseudovirus production system, along with cleavage and furin inhibition assays, immunoblotting and secondary structure prediction analysis, we show that Ca plays a key role in the processing efficien

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Gribling-Burrer, Anne-Sophie, Marco Chiabudini, Ying Zhang, et al. "A dual role of the ribosome-bound chaperones RAC/Ssb in maintaining the fidelity of translation termination." Nucleic Acids Research 47, no. 13 (2019): 7018–34. http://dx.doi.org/10.1093/nar/gkz334.

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Abstract The yeast ribosome-associated complex RAC and the Hsp70 homolog Ssb are anchored to the ribosome and together act as chaperones for the folding and co-translational assembly of nascent polypeptides. In addition, the RAC/Ssb system plays a crucial role in maintaining the fidelity of translation termination; however, the latter function is poorly understood. Here we show that the RAC/Ssb system promotes the fidelity of translation termination via two distinct mechanisms. First, via direct contacts with the ribosome and the nascent chain, RAC/Ssb facilitates the translation of stalling-p

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Shih, Jing-Wen, Wei-Ting Wang, Tsung-Yuan Tsai, Chu-Yun Kuo, Hao-Kang Li, and Yan-Hwa Wu Lee. "Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response." Biochemical Journal 441, no. 1 (2011): 119–29. http://dx.doi.org/10.1042/bj20110739.

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Upon environmental insults, SGs (stress granules) aid cell survival by serving as sites of translational silencing. RNA helicase DDX3 was reported to associate with SGs. However, its role in SG physiology remains undefined. We have demonstrated previously that DDX3 acts as an eIF4E (eukaryotic initiation factor 4E)-inhibitory protein to suppress translation. In the present study, we indentified the SG marker PABP1 [poly(A)-binding protein 1] as another direct interaction partner of DDX3. We established various stimuli as novel stressors that direct DDX3 with eIF4E and PABP1 into SGs, but not t

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Martin, Patricia E. M., Geraldine Blundell, Shoeb Ahmad, Rachel J. Errington, and W. Howard Evans. "Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels." Journal of Cell Science 114, no. 21 (2001): 3845–55. http://dx.doi.org/10.1242/jcs.114.21.3845.

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The assembly of gap junctions was investigated in mammalian cells expressing connexin (Cx) 26, 32 and 43 fused to green, yellow or cyan fluorescent proteins (GFP, YFP, CFP). Targeting of Cx32-CFP and 43-GFP to gap junctions and gap junctional communication was inhibited in cells treated with Brefeldin A, a drug that disassembles the Golgi. However gap junctions constructed of Cx26-GFP were only minimally affected by Brefeldin A. Nocodazole, a microtubule disruptor, had little effect on the assembly of Cx43-GFP gap junctions, but perturbed assembly of Cx26-GFP gap junctions. Co-expression of Cx

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28

Grskovic, M. "A co-repressor assembly nucleated by Sex-lethal in the 3'UTR mediates translational control of Drosophila msl-2 mRNA." EMBO Journal 22, no. 20 (2003): 5571–81. http://dx.doi.org/10.1093/emboj/cdg539.

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29

AHMAD, Shoeb, Juan A. DIEZ, Christopher H. GEORGE, and W. Howard EVANS. "Synthesis and assembly of connexins in vitro into homomeric and heteromeric functional gap junction hemichannels." Biochemical Journal 339, no. 2 (1999): 247–53. http://dx.doi.org/10.1042/bj3390247.

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The biogenesis of connexins and their assembly into functional gap junction hemichannels (connexons) was studied with the use of a cell-free transcription/translation system. Velocity sedimentation on sucrose gradients showed that a small proportion of connexin (Cx) 26 and Cx32 that were co-translationally translocated into microsomes were oligomers of Cx26 and Cx32. Chemical cross-linking studies showed that these corresponded to hexameric connexons. Reconstitution of connexons synthesized in vitro into liposomes induced permeability properties consistent with the view that open gap junction

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BRYANT, Jeffrey C., Ryan S. WESTPHAL та Brian E. WADZINSKI. "Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Bα subunit". Biochemical Journal 339, № 2 (1999): 241–46. http://dx.doi.org/10.1042/bj3390241.

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Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro, but the functional consequence(s) of this post-translational modification in the context of the cell remain unclear. Alkali-induced demethylation of PP2AC in purified PP2A heterotrimer (ABαC), but not in purified PP2A heterodimer (AC), indicated that a larger fraction of PP2AC is carboxymethylated in ABαC than in AC. To explore the role of Leu309 in PP2A holoenzyme assembly, epitope-tagged PP2A catalytic subunit (HA-PP2A)

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31

Harris, Nicola J., Kalypso Charalambous, Heather E. Findlay, and Paula J. Booth. "Lipids modulate the insertion and folding of the nascent chains of alpha helical membrane proteins." Biochemical Society Transactions 46, no. 5 (2018): 1355–66. http://dx.doi.org/10.1042/bst20170424.

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Membrane proteins must be inserted into a membrane and folded into their correct structure to function correctly. This insertion occurs during translation and synthesis by the ribosome for most α-helical membrane proteins. Precisely how this co-translational insertion and folding occurs, and the role played by the surrounding lipids, is still not understood. Most of the work on the influence of the lipid environment on folding and insertion has focussed on denatured, fully translated proteins, and thus does not replicate folding during unidirectional elongation of nascent chains that occurs in

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Kleizen, Bertrand, Marcel van Willigen, Marjolein Mijnders, et al. "Co-Translational Folding of the First Transmembrane Domain of ABC-Transporter CFTR is Supported by Assembly with the First Cytosolic Domain." Journal of Molecular Biology 433, no. 13 (2021): 166955. http://dx.doi.org/10.1016/j.jmb.2021.166955.

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SHU, H. H., and H. U. GÖRINGER. "Trypanosoma brucei mitochondrial ribonucleoprotein complexes which contain 12S and 9S ribosomal RNAs." Parasitology 116, no. 2 (1998): 157–64. http://dx.doi.org/10.1017/s0031182097002023.

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Antibiotics have been widely used to identify ribosomal activity in Trypanosoma brucei mitochondria. The validity of some of the results has been questioned because the permeability of the trypanosome cell membrane for some antibiotics was not adequately addressed. Here we describe translation inhibition experiments with digitonin-permeabilized trypanosomes to exclude diffusion barriers through the cell membrane. Using this system we were able to confirm, next to the eukaryotic and thus cycloheximide-sensitive translation system, the existence of a prokaryotic-type translational activity being

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Cockman, Eric, Paul Anderson, and Pavel Ivanov. "TOP mRNPs: Molecular Mechanisms and Principles of Regulation." Biomolecules 10, no. 7 (2020): 969. http://dx.doi.org/10.3390/biom10070969.

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The cellular response to changes in the surrounding environment and to stress requires the coregulation of gene networks aiming to conserve energy and resources. This is often achieved by downregulating protein synthesis. The 5’ Terminal OligoPyrimidine (5’ TOP) motif-containing mRNAs, which encode proteins that are essential for protein synthesis, are the primary targets of translational control under stress. The TOP motif is a cis-regulatory RNA element that begins directly after the m7G cap structure and contains the hallmark invariant 5’-cytidine followed by an uninterrupted tract of 4–15

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van den Biggelaar, Maartje, Birgit Ruhdorfer, Jan Voorberg, and Koen Mertens. "The Effect of von Willebrand Factor Normandy Mutations on Intracellular Co-Trafficking of Factor VIII to Storage Organelles." Blood 110, no. 11 (2007): 765. http://dx.doi.org/10.1182/blood.v110.11.765.765.

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Abstract Von Willebrand Factor (VWF) serves a critical role in factor (F)VIII life-cycle. It is generally believed that FVIII and VWF assemble in the circulation after secretion from vascular endothelial cells (VWF) and hepatocytes (FVIII). However, during the last few years evidence has accumulated in favor of significant extra-hepatic FVIII sources that might be compatible with in vivo biosynthesis and co-storage of FVIII and VWF within the same cell. In agreement with this view, in vitro co-expression of FVIII and VWF results in the storage of FVIII in VWF-containing organelles. We have rec

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Alzahrani, Nabeel, Ming-Jhan Wu, Saravanabalaji Shanmugam, and MinKyung Yi. "Delayed by Design: Role of Suboptimal Signal Peptidase Processing of Viral Structural Protein Precursors in Flaviviridae Virus Assembly." Viruses 12, no. 10 (2020): 1090. http://dx.doi.org/10.3390/v12101090.

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The Flaviviridae virus family is classified into four different genera, including flavivirus, hepacivirus, pegivirus, and pestivirus, which cause significant morbidity and mortality in humans and other mammals, including ruminants and pigs. These are enveloped, single-stranded RNA viruses sharing a similar genome organization and replication scheme with certain unique features that differentiate them. All viruses in this family express a single polyprotein that encodes structural and nonstructural proteins at the N- and C-terminal regions, respectively. In general, the host signal peptidase cl

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AHMAD, Shoeb, and W. Howard EVANS. "Post-translational integration and oligomerization of connexin 26 in plasma membranes and evidence of formation of membrane pores: implications for the assembly of gap junctions." Biochemical Journal 365, no. 3 (2002): 693–99. http://dx.doi.org/10.1042/bj20011572.

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Gap-junction channels provide a widespread intercellular signalling mechanism. They are constructed of a family of connexin membrane proteins that thread across the membrane four times and oligomerize to generate hexameric gap-junction hemichannels. Using an in vitro cell-free transcription/translation system, we demonstrate that connexin (Cx) 26, one of the smallest connexins, is integrated directly in a post-translational manner into plasma membranes. Protein-cleavage studies of Cx26 integrated into plasma membranes indicate a similar native transmembrane topography to that of Cx26 integrate

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Tagoug, Ines, Paola Neri, Jiri Slaby, et al. "XPO1 Inhibition Disrupts Ribosomal Subunits Assembly and Induces Multiple Myeloma (MM) Cell Death." Blood 122, no. 21 (2013): 3165. http://dx.doi.org/10.1182/blood.v122.21.3165.3165.

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Abstract Background Chromosomal region maintenance (CRM1), also known as exportin 1 (XPO1) plays an important role in the nuclear-cytoplasmic shuttling. The nuclear export receptor, XPO1, is considered as a regulator of subcellular distribution of several proteins involved in the regulation of centrosome duplication such as nucleophosmin (NPM), breast and ovarian cancer susceptibility protein 1 (BRCA1) and many tumor suppressor proteins (p53, p21, FOXO and pRB). Furthermore, XPO1 is required for the export of assembled ribosomal subunits (60S & 40S) from the nucleolus back into the cytopla

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Pal, Ipsita, Sohani Das Sharma, Andre M. Grilo, et al. "Casein Kinase 1 Delta Is a Novel Regulator of mRNA Translation and Druggable Target in Aggressive Lymphomas." Blood 134, Supplement_1 (2019): 2864. http://dx.doi.org/10.1182/blood-2019-129528.

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Introduction: As an oncoprotein and a transcription factor, C-MYC has been extensively validated as a key driver in many cancers such as Diffuse large B cell lymphoma (DLBCL). C-MYC has been intensely investigated as a therapeutic target in preclinical models. However, no drugs have been successfully developed to target c-Myc, and the c-Myc oncoprotein has been recognized as undruggable. Recent data from our lab and others suggest that the translation of C-MYC and some other oncogenes may be preferentially repressed using inhibitors of the translation apparatus. Translation can be divided into

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Burrone, Oscar R., José L. Slon Campos, Monica Poggianella, and Jyoti Rana. "Impact of Capsid Anchor Length and Sequential Processing on the Assembly and Infectivity of Dengue Virus." Proceedings 50, no. 1 (2020): 32. http://dx.doi.org/10.3390/proceedings2020050032.

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: The assembly and secretion of flaviviruses are part of an elegantly regulated process. During maturation, the viral polyprotein undergoes several co- and post-translational cleavage events mediated by both viral and host proteases. Among these, sequential cleavage at the N- and C-termini of the hydrophobic capsid anchor (Ca) at the junction of C-PrM has been considered essential for the production of flaviviruses. Here, using a refined dengue pseudovirus production system, we show that Ca plays a key role in the processing efficiency of dengue virus type 2 (DENV2) structural proteins and the

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Lee, G., and S. L. Rook. "Expression of tau protein in non-neuronal cells: microtubule binding and stabilization." Journal of Cell Science 102, no. 2 (1992): 227–37. http://dx.doi.org/10.1242/jcs.102.2.227.

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The microtubule-associated protein tau is a developmentally regulated family of neuronal phosphoproteins that promotes the assembly and stabilization of microtubules. The carboxy-terminal half of the protein contains three copies of an imperfectly repeated sequence; this region has been found to bind microtubules in vitro. In addition, a fourth copy of the repeat has been found in adult-specific forms of tau protein. To examine the structure and function of tau protein in vivo, we have transiently expressed fetal and adult forms of tau protein and tau protein fragments in tissue culture cells.

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Ikeda, Marina, Akihiro Ito, Yuichi Sekine, and Masahiro Fujimuro. "UBE1a Suppresses Herpes Simplex Virus-1 Replication." Viruses 12, no. 12 (2020): 1391. http://dx.doi.org/10.3390/v12121391.

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Herpes simplex virus-1 (HSV-1) is the causative agent of cold sores, keratitis, meningitis, and encephalitis. HSV-1-encoded ICP5, the major capsid protein, is essential for capsid assembly during viral replication. Ubiquitination is a post-translational modification that plays a critical role in the regulation of cellular events such as proteasomal degradation, protein trafficking, and the antiviral response and viral events such as the establishment of infection and viral replication. Ub-activating enzyme (E1, also named UBE1) is involved in the first step in the ubiquitination. However, it i

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Phan, Tamara, Fatima Khalid, and Sebastian Iben. "Nucleolar and Ribosomal Dysfunction—A Common Pathomechanism in Childhood Progerias?" Cells 8, no. 6 (2019): 534. http://dx.doi.org/10.3390/cells8060534.

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The nucleolus organizes around the sites of transcription by RNA polymerase I (RNA Pol I). rDNA transcription by this enzyme is the key step of ribosome biogenesis and most of the assembly and maturation processes of the ribosome occur co-transcriptionally. Therefore, disturbances in rRNA transcription and processing translate to ribosomal malfunction. Nucleolar malfunction has recently been described in the classical progeria of childhood, Hutchinson–Gilford syndrome (HGPS), which is characterized by severe signs of premature aging, including atherosclerosis, alopecia, and osteoporosis. A der

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Falcone, Domina, Matthew P. Henderson, Hendrik Nieuwland, Christine M. Coughlan, Jeffrey L. Brodsky, and David W. Andrews. "Stability and function of the Sec61 translocation complex depends on the Sss1p tail-anchor sequence." Biochemical Journal 436, no. 2 (2011): 291–303. http://dx.doi.org/10.1042/bj20101865.

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Sss1p, an essential component of the heterotrimeric Sec61 complex in the ER (endoplasmic reticulum), is a tail-anchored protein whose precise mechanism of action is largely unknown. Tail-anchored proteins are involved in many cellular processes and are characterized by a single transmembrane sequence at or near the C-terminus. The Sec61 complex is the molecular machine through which secretory and membrane proteins translocate into and across the ER membrane. To understand the function of the tail anchor of Sss1p, we introduced mutations into the tail-anchor sequence and analysed the resulting

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Zhang, Yue, Zeyu Wen, Xuemeng Shi, Yan-Jun Liu, John E. Eriksson, and Yaming Jiu. "The diverse roles and dynamic rearrangement of vimentin during viral infection." Journal of Cell Science 134, no. 5 (2020): jcs250597. http://dx.doi.org/10.1242/jcs.250597.

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ABSTRACTEpidemics caused by viral infections pose a significant global threat. Cytoskeletal vimentin is a major intermediate filament (IF) protein, and is involved in numerous functions, including cell signaling, epithelial–mesenchymal transition, intracellular organization and cell migration. Vimentin has important roles for the life cycle of particular viruses; it can act as a co-receptor to enable effective virus invasion and guide efficient transport of the virus to the replication site. Furthermore, vimentin has been shown to rearrange into cage-like structures that facilitate virus repli

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Johnson, Jillian G., Matthew R. Paul, Casey D. Kniffin, Paul E. Anderson, Louis E. Burnett, and Karen G. Burnett. "High CO2 alters the hypoxia response of the Pacific whiteleg shrimp (Litopenaeus vannamei) transcriptome including known and novel hemocyanin isoforms." Physiological Genomics 47, no. 11 (2015): 548–58. http://dx.doi.org/10.1152/physiolgenomics.00031.2015.

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Acclimation to low O2 in many organisms involves changes at the level of the transcriptome. Here we used high-throughput RNA sequencing (RNA-Seq) to explore the global transcriptomic response and specific involvement of a suite of hemocyanin (Hc) subunits to low O2 alone and in combination with high CO2, which naturally co-occurs with low O2. Hepatopancreas mRNA of juvenile L. vannamei exposed to air-saturated water, low O2, or low O2/high CO2 for 4 or 24 h was pooled, sequenced (HiSeq 2500) and assembled (Trinity: 52,190 contigs) to create a deep strand-specific reference transcriptome. Annot

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Needham, David M., Camille Poirier, Elisabeth Hehenberger, et al. "Targeted metagenomic recovery of four divergent viruses reveals shared and distinctive characteristics of giant viruses of marine eukaryotes." Philosophical Transactions of the Royal Society B: Biological Sciences 374, no. 1786 (2019): 20190086. http://dx.doi.org/10.1098/rstb.2019.0086.

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Giant viruses have remarkable genomic repertoires—blurring the line with cellular life—and act as top–down controls of eukaryotic plankton. However, to date only six cultured giant virus genomes are available from the pelagic ocean. We used at-sea flow cytometry with staining and sorting designed to target wild predatory eukaryotes, followed by DNA sequencing and assembly, to recover novel giant viruses from the Pacific Ocean. We retrieved four ‘PacV’ partial genomes that range from 421 to 1605 Kb, with 13 contigs on average, including the largest marine viral genomic assembly reported to date

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Penn, E. J., C. Hobson, D. A. Rees, and A. I. Magee. "Structure and assembly of desmosome junctions: biosynthesis, processing, and transport of the major protein and glycoprotein components in cultured epithelial cells." Journal of Cell Biology 105, no. 1 (1987): 57–68. http://dx.doi.org/10.1083/jcb.105.1.57.

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Extracts of metabolically labeled cultured epithelial cells have been analyzed by immunoprecipitation followed by SDS-PAGE, using antisera to the major high molecular mass proteins and glycoproteins (greater than 100 kD) from desmosomes of bovine muzzle epidermis. For nonstratifying cells (Madin-Darby canine kidney [MDCK] and Madin-Darby bovine kidney), and A431 cells that have lost the ability to stratify through transformation, and a stratifying cell type (primary human keratinocytes) apparently similar polypeptides were immunoprecipitated with our antisera. These comprised three glycoprotei

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Pogoda, Elzbieta, Hanna Tutaj, Adrian Pirog, Katarzyna Tomala, and Ryszard Korona. "Overexpression of a single ORF can extend chronological lifespan in yeast if retrograde signaling and stress response are stimulated." Biogerontology 22, no. 4 (2021): 415–27. http://dx.doi.org/10.1007/s10522-021-09924-z.

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AbstractSystematic collections of single-gene deletions have been invaluable in uncovering determinants of lifespan in yeast. Overexpression of a single gene does not have such a clear outcome as cancellation of its function but it can lead to a variety of imbalances, deregulations and compensations, and some of them could be important for longevity. We report an experiment in which a genome-wide collection of strains overexpressing a single gene was assayed for chronological lifespan (CLS). Only one group of proteins, those locating to the inner membrane and matrix of mitochondria, tended to

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Claessens, Frank, and Daniel T. Gewirth. "DNA recognition by nuclear receptors." Essays in Biochemistry 40 (June 1, 2004): 59–72. http://dx.doi.org/10.1042/bse0400059.

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The nuclear receptors constitute a large family of ligand-inducible transcription factors. The control of many genetic pathways requires the assembly of these nuclear receptors in defined transcription-activating complexes within control regions of ligand-responsive genes. An essential step is the interaction of the receptors with specific DNA sequences, called hormone-response elements (HREs). These response elements position the receptors, and the complexes recruited by them, close to the genes of which transcription is affected. HREs are bipartite elements that are composed of two hexameric

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