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1

Roberson, Jerry R., Lawrence K. Fox, Dale D. Hancock, John M. Gay, and Thomas E. Besser. "Prevalence of coagulase-positive staphylococci, other than Staphylococcus aureus, in bovine mastitis." American Journal of Veterinary Research 57, no. 1 (1996): 54–58. http://dx.doi.org/10.2460/ajvr.1996.57.01.54.

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Abstract Objective To determine prevalence and relevance of coagulase-positive Staphylococcus hyicus and S intermedius intramammary infections (IMI) in dairy cows and determine the ability of the 4-hour tube coagulase (TC) test to differentiate the coagulase-positive staphylococci (CPS). Design Prevalence of CPS was determined for primiparous cows (point prevalence and prevalence at first parturition) and multiparous cows (point prevalence) of 2 herd groups: < 6% CPS IMI prevalence = low prevalence (LP); > 10% CPS IMI prevalence = high prevalence (HP). Sample population For prevalence, c
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2

Hogan, Joseph S., Angelyn Cornetta, and J. Woodrow Pankey. "Comparison of four test procedures to identify Staphylococcus aureus isolated from bovine intramammary infections." American Journal of Veterinary Research 47, no. 9 (1986): 2017–19. https://doi.org/10.2460/ajvr.1986.47.09.2017.

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SUMMARY A comparison was made between conventional tube coagulase, macrocupsular coagulase, latex agglutination, and miniaturized biochemical test systems for identification of Staphylococcus aureus of bovine origin. A total of 303 gram-positive, catalase-positive cocci of bovine origin were tested. Agreement between each pair of 4-hour tube coagulase, macrocupsular coagulase, latex agglutination, and miniaturized biochemical test results within isolates was > 95.0. Seventeen (5.6%) isolates were test negative for 4-hour tube coagulase, but test positive for 24-hour tube coagulase. Thirteen
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3

Belmamoun, Ahmed Reda, Abdelkader Ammam, Imene Berrabah, and Karima Bereksi Reguig. "Coagulase gene polymorphism and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine subclinical mastitis milk in Sidi-Bel-Abbes, Algeria." South Asian Journal of Experimental Biology 7, no. 1 (2017): 21–27. http://dx.doi.org/10.38150/sajeb.7(1).p21-27.

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The study was conducted to identify and characterize Staphylococcus aureus in raw milk derived from subclinical mastitis in Sidi-Bel-Abbes Algeria. In this paper, we explore the possibility of detection of the coagulase gene (coa), which encodes the coagulase enzyme, by PCR analysis in antibiotic-resistant isolates, with the latex agglutination phenotype and free coagulase.Out of 336 samples of raw milk examined with California mastitis test (CMT) posi-tive; a total of 142 samples were bacteriologically positive with 56.34% Staphylococcus isolates, 21 (26.25%) isolates were confirmed as S.aure
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4

BEATTY, BRUCE R., RALPH J. FARNSWORTH, ARNOLD J. LUND, RICHARD H. LYON, and GILBERT E. WARD. "Medium to Culture and Differentiate Coagulase-Positive and -Negative Staphylococci from Bovine Milk." Journal of Food Protection 48, no. 12 (1985): 1019–21. http://dx.doi.org/10.4315/0362-028x-48.12.1019.

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A medium which incorporates CAMP factor produced by Streptococcus agalactiae (group B) into sheep blood agar was used to culture and identify coagulase-positive staphylococci from bovine milk. Of 506 staphylococcal isolates from bovine milk, 92.5% of coagulase-positive organisms produced a wide zone of complete hemolysis, whereas 98.9% of coagulase-negative organisms did not. The agreement of this one-step culture and identification test with the standard tube coagulase test was higher than that of the deoxyribonuclease test medium, Baird-Parker egg yolk medium, tellurite glycine medium and sl
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5

Sharma, Binita Koirala, S. Gokhale, and K. Sharma. "Urea-Fibrinogen Slide Coagulase Test – A Simple Alternative Method for the Rapid Identification of Staphylococcus aureus." Journal of Gandaki Medical College-Nepal 10, no. 1 (2017): 5–10. http://dx.doi.org/10.3126/jgmcn.v10i1.17903.

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Introduction: The accurate identification of Staphylococcus aureus clinical isolates requires a series of tests. Morphological features and slide coagulase test are two criteria on which S. aureus are identified. Resort to tube coagulase test is sought when results of slide coagulase test are equivocal or doubtful. Both coagulase tests detect the enzymes that convert fibrinogen into fibrin. Human, rabbit or sheep pooled plasma is used as substrate for both tests. Slide coagulase test is simpler and faster as compared to tube coagulase test. The plasma could be carrier of many human and animal
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6

Onemu, S. O., M. O. Onemu-Metitiri, and Emmanuel Ifeanyi Obeagu. "Prevalence of Coagulase Negative Strains of Staphylococcus aureus in Clinical Specimens at the University of Benin Teaching Hospital, Benin City, Nigeria." Asian Journal of Research in Infectious Diseases 14, no. 4 (2023): 49–53. http://dx.doi.org/10.9734/ajrid/2023/v14i4306.

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The test for coagulase activity is a simple and non-expensive test for the identification of S. aureus strains from clinical samples in resource-poor settings. The results of these tests have been reported to vary with the source of plasma and with some atypical strains that test negative with the coagulase test. The study was carried out to determine if misidentification of strains of S. aureus exists on account of the reliance on the coagulase tests. Clinical isolates of Staphylococci that tested negative by the coagulase tests from wounds, pus, aspirates, blood cultures and urogenital sampl
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7

Chang, Tsung C., Su H. Huang, H. Y. Chao, et al. "Efficacy of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus: Collaborative Study." Journal of AOAC INTERNATIONAL 79, no. 3 (1996): 661–70. http://dx.doi.org/10.1093/jaoac/79.3.661.

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Abstract Fifteen laboratories completed a collaborative study comparing the efficacy of a latex agglutination kit (Aureus Test) with that of AOAC Official Method 987.09 (coagulase test for identification of Staphylococcus aureus). Each laboratory analyzed 240 strains of bacteria, including 160 isolates of S. aureus and 80 isolates of other bacteria. Upon receipt of cultures, collaborators subcultured each isolate on both tryptic soy agar (TSA) and Baird-Parker agar medium (BPA) to determine whether the growth medium has any effect on either method. For cultures grown on TSA, the latex test had
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8

Hall, Gerri S., Kathleen Pratt, Gail Woods, and Cynthia Corlett-Knapp. "Differentiation of Staphylococcus aureus From Other Micrococcaceae: Comparison of Staphaurex and the Slide Coagulase Test With the Tube Coagulase Test." Laboratory Medicine 19, no. 12 (1988): 817–20. http://dx.doi.org/10.1093/labmed/19.12.817.

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9

CHANG, TSUNG C., and SU H. HUANG. "Modification of the Conventional Procedure for the Test of Staphylococcal Coagulase." Journal of Food Protection 59, no. 2 (1996): 197–98. http://dx.doi.org/10.4315/0362-028x-59.2.197.

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The coagulase test is routinely used for the confirmation of suspect Staphylococcus aureus on Baird-Parker agar (BPA). Overnight (18 to 24 h) preincubation of suspect colonies in brain heart infusion (BHI) broth is generally practiced prior to the coagulase test. In order to shorten the test protocol, different preincubation times of S. aureus in BHI were evaluated. Stock cultures (257 strains) of S. aureus were subcultured on BPA, and single colonies of each strain on BPA were analyzed for coagulase activities by the conventional procedure except that the preincubation times in BHI were varie
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10

Coimbra-e-Souza, V., M. A. V. P. Brito, R. C. Chamon, M. S. Laport, and M. Giambiagi-deMarval. "Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69, no. 6 (2017): 1669–75. http://dx.doi.org/10.1590/1678-4162-9351.

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ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molec
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11

Akineden, Ömer, Abdulwahed Ahmed Hassan, Elisabeth Schneider, and Ewald Usleber. "A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk." Journal of Dairy Research 78, no. 1 (2010): 38–42. http://dx.doi.org/10.1017/s0022029910000774.

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Bacteriological analysis of milk samples from quarters of a dairy cow suffering from subclinical mastitis yielded two isolates ofStaphylococcus aureuswhich gave a negative reaction in the standard coagulase test. Both isolates were also clumping factor and thermonuclease negative, and gave a negative reaction in the Staphaurex®test. The isolates were identified by using commercial biochemical systems, and by PCR analysis of different staphylococcal cell surface protein and exoprotein genes. Further molecular identification of the isolates, which included sequencing of the 16S rRNA gene and RT-
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12

Shah, Pradeep Kumar, Niru Bhandari, Basanta Tamang, and Rajendra D. Joshi. "Antibiotic Susceptibility and Biofilm Production among Coagulase Negative Staphylococci Isolated from Clinical Samples at Tertiary Care Hospital." Journal of Nepal Health Research Council 21, no. 4 (2024): 636–41. http://dx.doi.org/10.33314/jnhrc.v21i4.4894.

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Background: Coagulase Negative Staphylococci have been widely associated with medical device implant treatment and immune-compromised patients. Despite having increasing interest in Coagulase Negative Staphylococci, few studies from Nepal have reported the association of these organisms with urinary tract infections, conjunctivitis, high vaginal swabs, and cerebrospinal fluid. This study was carried out to determine antibiotic susceptibility pattern and biofilm production among Coagulase Negative Staphylococci isolated from clinical samples at tertiary care hospital.Methods: This study was a h
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13

Boipai, Manju, Krishna Chander Birua, N. P. Sahu, L. B. Pandey, Rameswari Beck, and Shashi Dinkar. "Microbiological and Biochemical Analysis of Methicillin-resistant Staphylococcus aureus Isolated from Patients Admitted in RIMS, Ranchi." Asian Pacific Journal of Health Sciences 7, no. 3 (2020): 39–43. http://dx.doi.org/10.21276/apjhs.2020.7.3.10.

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Background: Since methicillin-resistant Staphylococcus aureus strains are resistant to multiple antibiotics, there is a possibility of extensive outbreaks which may be difficult to control. Early detection of methicillin-resistant Staphylococcus is important from patients and hospitals point of view. Materials and Methods: The present study was carried out in the Department of Microbiology, Rajendra Institute of Medical Sciences, Ranchi, clinical isolates of methicillin-resistant S. aureus strains were obtained from admitted patients of Rajendra Institute of Medical Sciences, Ranchi. The sourc
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14

Shahmohammadi, Saeed, Ahmad Farajzadeh Sheikh, Mojtaba Shahin, and Iman Mir. "Evaluation of Antibiotic Susceptibility Pattern and Distribution of coa Genes in Coagulase-negative S. aureus from Ahvaz, Iran." Infectious Disorders - Drug Targets 19, no. 4 (2019): 383–87. http://dx.doi.org/10.2174/1871526518666180816101629.

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Background: Staphylococcus aureus is one of the important causes of clinical infections that can be more destructive by its antibiotic resistant strains. Objective: This study aimed to determine the antibiotic susceptibility pattern and distribution of mecA and coa genes in clinical isolates of S. aureus. Methods: Two hundred seventy-three specimens suspected to S. aureus were taken from hospitals of Ahvaz, southwest of Iran. Isolates were identified by standard microbiologic tests and confirmed by the molecular method. Antimicrobial susceptibility testing was carried out by disk diffusion met
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15

Effendi, Mustofa Helmi. "PEMBUKTIAN HORIZONTAL TRANSFER OF RESISTANCE GENES MELALUI UJI SENSITIVITAS ANTIBIOTIKA PADA BAKTERI GENUS Staphylococcus DARI KASUS BOVINE MASTITIS." Berkala Penelitian Hayati 13, no. 2 (2008): 187–92. http://dx.doi.org/10.23869/bphjbr.13.2.200815.

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The experiment was carried out to show the evidence of horizontal transfer of resistance genes by antibiotics sensitivity test on bacteria of genus of Staphylococcus from bovine mastitic cases. The first step of the experiment was to prepare pure culture of Staphylococcus aureus and Coagulase Negative Staphylococci. Milk samples were collected from mastitic cases at the afternoon milking time. Preparation of pure culture of isolates were confirmed by MS agar, hemolytic activity, catalase and coagulase test. Both isolates were tested by using antibiotics sensitivity test. The result showed that
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16

Hueston, William D., Nolan R. Hartwig, and John K. Judy. "Detection of ovine intramammary infection with the California mastitis test." Journal of the American Veterinary Medical Association 188, no. 5 (1986): 522–24. https://doi.org/10.2460/javma.1986.188.05.522.

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SUMMARY The overall sensitivity of the California mastitis test (cmt) for detecting intramammary infection (the likelihood of a positive cmt score in the presence of intramammary infection) was 69.3%. The specificity of the cmt (likelihood of a negative test in the absence of intramammary infection) was 76.5%. When only infections by major ovine mastitis pathogens were considered, the sensitivity increased to 100% and the specificity decreased to 71.1%. Bacterial cultural examination and cmt scores were recorded for 526 samples of milk obtained from 106 brood ewes. Positive cmt scores were rec
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17

Yigit, N., AE Aktas, and A. Ayyildiz. "Detection of Coagulase Activity in Pathogenic Candida Species." Journal of International Medical Research 36, no. 6 (2008): 1378–82. http://dx.doi.org/10.1177/147323000803600627.

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The coagulase activities of various Candida spp were assessed using a classical tube test. A total of 125 clinical Candida isolates representing eight species ( C. albicans, C. parapsilosis, C. tropicalis, C. glabrata, C. kefyr, Geotrichum candidum, C. krusei and C. guilliermondii) were incubated with rabbit, sheep or human plasma. After 24 h incubation, 29/64 (45.3%) C. albicans isolates and 23/61 (37.7%) non-albicans Candida strains were positive for coagulase in rabbit plasma, whereas 20/64 (31.2%) C. albicans and 6/61 (9.8%) non-albicans Candida isolates were positive for coagulase in shee
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18

Mohammed, Suhad Hadi, Mohammed Neama Hmood, and Areej Abbas Abd. "Screening of nasal carriage for Staphylococcus aureus and their resistance to oxacillin and cefoxitin among medical students in Karbala University." Journal of Contemporary Medical Sciences 1, no. 1 (2015): 13–16. http://dx.doi.org/10.22317/jcms.v1i1.56.

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Objective Carriage of Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA) is a problem within healthcare organizationsand in the community. The aim of the study was to screen S. aureus carriage and their susceptibility to cefoxitin and oxacillin amongmedical students.Methods A total of 100 nasal swabs were collected. Isolation and identification of the isolate as S. aureus was done using Gram stain,coagulase test and catalase test. S. aureus isolates were confirmed as MRSA using cefoxitin (30 μg) disc and oxacillin (30 μg) disc by Kirby-Bauer disc diffusion method on Mue
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19

Al-Araji, Mohammed kadum. "New Technique use to differentiate between Pseudo and true coagulase Activity of Staphylococcus aureus isolated from Different infected Sites in Patients from Baghdad Hospitals." Al Mustansiriyah Journal of Pharmaceutical Sciences 11, no. 1 (2012): 163–76. http://dx.doi.org/10.32947/ajps.v11i1.250.

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A total of 300 strains of Staphylococci isolated from various pathological sites of infection from different hospitals; these specimens derived from cases ofhuman infections were tested for staphylocoagulase activity.
 Test systems employing normal citrated rabbit plasma and the same substrate supplemented with inhibitors of thrombin and proteolytic enzymes (but notinfluencing the staphylocoagulase activity) were used for testing suspensions of bacteria and cell-free culture supernatants. A total of 200 strains clotted normal rabbit plasma; however addition of Trasylol and heparine result
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20

BENNETT, REGINALD W. "Atypical Toxigenic Staphylococcus and Non-Staphylococcus aureus Species on the Horizon? An Update†." Journal of Food Protection 59, no. 10 (1996): 1123–26. http://dx.doi.org/10.4315/0362-028x-59.10.1123.

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Staphylococcal food poisoning is a commonly reported illness caused by the ingestion of preformed staphylococcal enterotoxin in foods, With some exceptions, enterotoxin production is associated with coagulase-positive rather than coagulase-negative staphylococci. Of the coagulase-positive staphylococcal species, S. aureus was historically thought to be exclusively implicated in human foodborne illness. More recently, however, other coagulase-positive and some coagulase-negative staphylococcal species have been associated with foodborne intoxication, Coagulase activity has been used to indicate
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21

CHANG, TSUNG C., and SU H. HUANG. "Evaluation of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus from Foods." Journal of Food Protection 56, no. 9 (1993): 759–62. http://dx.doi.org/10.4315/0362-028x-56.9.759.

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A latex agglutination kit (AUREUS TEST™) for the rapid identification of Staphylococcus aureus was evaluated in this study. The reagent consists of polystyrene latex particles sensitized with rabbit anti-protein A immunoglobulin G and fibrinogen. Due to the respective binding of bacterial protein A with immunoglobulin G and coagulase with fibrinogen, an agglutination reaction occurs within 1 min when the latex is mixed with a suspension of S. aureus. Of 157 S. aureus isolates (138 from foods) and 110 non-S. aureus isolates (58 species belonging to 19 genera), the sensitivity and specificity of
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22

Frebourg, Noëlle Barbier, Delphine Nouet, Ludovic Lemée, Emmanuelle Martin, and Jean-François Lemeland. "Comparison of ATB Staph, Rapid ATB Staph, Vitek, and E-Test Methods for Detection of Oxacillin Heteroresistance in Staphylococci Possessing mecA." Journal of Clinical Microbiology 36, no. 1 (1998): 52–57. http://dx.doi.org/10.1128/jcm.36.1.52-57.1998.

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The performance characteristics of the E-test (AB Biodisk, Solna, Sweden), the ATB Staph, the Rapid ATB Staph, and the Vitek GPS-503 card (bioMérieux, La Balme Les Grottes, France) methods for the detection of oxacillin resistance in a collection of staphylococci with a high proportion of troublesome strains were evaluated. Sixty-fourStaphylococcus aureus strains and 76 coagulase-negative staphylococcal strains were tested. All strains were mecApositive and were characterized by the oxacillin agar screen plate test; 75 (53.6%) were found to be heterogeneous by a large-inoculum oxacillin disk
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23

Pastoril, Maria Feibe M. "Inhibitory activity of Plumeria rubra (Kalachuchi), Ipomea quatica (Kangkong), Mimosa pudica (Makahiya), Euphorbia hirta (Gatas-Gatas) and Coleus aromaticus (Oregano) plant extracts against Staphylococcus aureus coagulase production." University of the Visayas - Journal of Research 8, no. 1 (2014): 27–36. https://doi.org/10.5281/zenodo.1961530.

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The leaf extracts of P. rubra (Kalachuchi), I. aquatica (Kangkong), M. pudica (Makahiya), E. hirta (Gatas-Gatas), and C. aromaticus (Oregano) were screened for inhibitory activity against coagulase production of S. aureus. The Tube Coagulase Test and Colony Count were used for the inhibitory assay. The E. hirta (Gatas-Gatas), and C. aromaticus (Oregano) were found to have inhibitory activity against S. aureus coagulase production both having a mean grade level of 1 and a mean colony count of 193.33 and 229.67, respectively. However, the in vitro tests conduct
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24

Case, Lisa C., Gerald V. Ling, Ernst L. Biberstein, Annette L. Ruby, and Spencer S. Jang. "Staphylococci in canine urolithiasis: Species identification, using a commercially available tray micromethod." American Journal of Veterinary Research 46, no. 1 (1985): 238–41. https://doi.org/10.2460/ajvr.1985.46.01.238.

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SUMMARY Fifty-one coagulase-positive staphylococcal isolates from canine urinary calculi or from the urine of dogs with documented urolithiasis, and 17 coagulase-positive staphylococcal isolates from human beings and cattle were identified by a commercially available tray micromethod, as well as by conventional methods. Canine isolates had previously been classified as Staphylococcus aureus on the basis of a positive tube coagulase test. After 5 hours’ incubation, the tray method identified all 51 canine urolithiasis isolates as S intermedius, rather than S aureus. All human and bovine isolate
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25

Jorgensen, J. H., M. L. McElmeel, and C. W. Trippy. "In vitro activities of the oxazolidinone antibiotics U-100592 and U-100766 against Staphylococcus aureus and coagulase-negative Staphylococcus species." Antimicrobial Agents and Chemotherapy 41, no. 2 (1997): 465–67. http://dx.doi.org/10.1128/aac.41.2.465.

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U-100592 and U-100766 are closely related antibiotics of the oxazolidinone class. Their in vitro activities were determined against 100 isolates of Staphylococcus aureus and 100 isolates of coagulase-negative Staphylococcus species by broth and agar dilution test methods. The MICs of both compounds by either test method at which 50 and 90% of isolates are inhibited were 2 and 4 micrograms/ml, respectively, for S. aureus and 1 to 2 micrograms/ml for coagulase-negative staphylococci. Time-kill assay with selected strains indicated a primarily bacteriostatic effect against staphylococci.
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26

BUCUR, Iulia Maria, Viorel HERMAN, Corina PASCU, Ionita IANCU, Janos DEGI, and Nicolae CATANA. "Research on the Phenotypic Characterization of Mrsa Strains Isolated from Animals." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine 74, no. 1 (2017): 88. http://dx.doi.org/10.15835/buasvmcn-vm:12610.

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Keywords: chromogen, methicillin, MRSA, resistanceIntroduction: Currently, both in staphylococci isolated from animals with different diseases, as well as in humans, the MRSA strains (Methicillin Resistant S. aureus) are monitored, as the methicillin resistance is associated with the resistance to other antibiotic groups.Methicillin resistance is encoded by mec staphylococcal chromosomal cassettes (SCCmec), which are islands of resistance. These strains can be identified by molecular biology tests and tests that reveal several phenotypic characteristics.The research was made in order to charac
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27

Maisi, Päivi, та Inna Riipinen. "Use of California Mastitis Test, N-acetyl-β-glucosaminidase, and antitrypsin to diagnose caprine subclinical mastitis". Journal of Dairy Research 55, № 3 (1988): 309–14. http://dx.doi.org/10.1017/s0022029900028569.

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SummaryAnalysis of 448 milk samples (11 herds) from caprine udder halves showed that microorganisms were isolated from 21·8% of the samples. California Mastitis Test (GMT) and N-acetyl-β-glucosaminidase (NAGase) were superior to antitrypsin in detecting subclinical infections. Coagulase-negative staphylococci and micrococci were the main species isolated from halves showing no clinical disease. Coagulase-positive staphylococcal infections were associated with a significant increase of all inflammatory parameters. Significantly increased CMT and NAGase occurred when streptococci, other staphylo
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28

Stroich, V. V., and Y. V. Horiuk. "Identification of the skin microbiota of healthy dogs and those with pyoderma." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 25, no. 110 (2023): 46–53. http://dx.doi.org/10.32718/nvlvet11008.

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Among the diseases of dogs, pyoderma is a frequent reason for referral to veterinary clinics. To effectively treat pyoderma and develop new strategies for preventing this disease, it is necessary to study the causes of formation and the conditions for the manifestation of pathogenic properties in causative agents. The purpose of the study will be to identify the microbiota isolated from the healthy skin of dogs and those with pyoderma. Washes were collected from the skin of various areas in clinically healthy dogs and dogs with pyoderma. The material was sown on selective media for the isolati
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29

Shuyang, Xu, and Yu Qiang. "Bacterial factors of mastitis in lactating women and its effect on the physical properties and chemical composition of breast milk." Cellular and Molecular Biology 67, no. 3 (2021): 172–77. http://dx.doi.org/10.14715/cmb/2021.67.3.27.

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Mastitis is a complication seen in some breastfeeding mothers and is the most common inflammatory lesion of the breast in breastfeeding mothers. In this complication, breast milk undergoes chemical and physical changes. It can lead to a drop in breastfeeding, weight loss, and, consequently, stunted growth of infants. Bacteria are the main cause of breast inflammation. Therefore, in this study, bacterial factors of mastitis were evaluated in lactating women. Also, their effects were considered on the physical properties and chemical composition of mothers' breast milk. For this purpose, 210 bre
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Widianingsih, Mastuti, and Dian Catur Setyorini. "Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri." Bioeksperimen: Jurnal Penelitian Biologi 5, no. 2 (2019): 99–105. http://dx.doi.org/10.23917/bioeksperimen.v5i2.9236.

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Contamination of processed beef foods such as abon can be caused by various types of microbes, one of which is Staphylococcus aureus. Staphylococcus aureus can cause various infections, both on the skin, gastrointestinal tract, or endocarditis. The objective of this research was to determine the presence of Staphylococcus aureus in beef abon sold in Pahing Market, Kediri. Abon used is non-branded beef abon which is as many as 10 samples obtained by total sampling technique. Samples were tested by observation of colony morphology through Gram staining, mannitol fermentation test, catalase and c
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31

Savic-Rajic, Natasa, and Vera Katic. "Susceptibility of coagulase positive staphylococci isolated from cow's mammary gland to antibacterial drugs." Veterinarski glasnik 63, no. 5-6 (2009): 299–310. http://dx.doi.org/10.2298/vetgl0906299s.

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Coagulase positive staphylococci are one of the most common causes of chronic udder infection. Indiscriminate use of antimicrobial drugs and their presence in the environment where animals live has led to coagulase positive staphylococci strains resistant to antimicrobial means. Proper and timely treatment of sub-clinical mastitis, based on the most effective use of antimicrobial drugs, is the key to good health of the milk herd. The aim was to determine the antimicrobial efficacy of selected assets in relation to coagulase positive staphylococci isolated from samples of milk taken from indivi
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Marta Setiabudy, Dewa Ayu Putri Sri Masyeni, Anak Agung Gede Indraningrat, Kadek Suryawan, I Ketut Agus Indra Adhiputra, and Muhammad Amirul bin Abdul Rahman. "Biofilm Formation in Staphylococcus aureus and Coagulase-Negative Staphylococcus." Folia Medica Indonesiana 59, no. 3 (2023): 222–28. http://dx.doi.org/10.20473/fmi.v59i3.44598.

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Highlights: 1. The significance of Staphylococcus aureus and coagulase-negative Staphylococcus, which are more likely to infectimmunocompromised patients, needed to be researched in greater depth.2. Coagulase-negative Staphylococcus was found to form significantly more biofilm than Staphylococcus aureus.3. Wound care and changing medical devices in immunocompromised patients on a regular basis may provide benefitsto prevent biofilm formation by Staphylococcus spp. Abstract Staphylococcus spp. are typically commensal microorganisms that can exist in the human body without causing illness. Howev
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Kaçmaz, Birgül, Serdar Gül, Doğan Barış Öztürk, and Emine Ecemiş. "Investigation of optimum temperature for coagulase test in Stahylococcus aureus strains." Turkish Bulletin of Hygiene and Experimental Biology 72, no. 2 (2015): 99–102. http://dx.doi.org/10.5505/turkhijyen.2015.22438.

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IJAR, Afreen, Bhattacharyya S, Sengupta A, et al. "Identification of Candida and Trichosporon species by oxidase and coagulase test." International Journal of Advanced Research 4, no. 5 (2016): 475–749. http://dx.doi.org/10.21474/ijar01/393.

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SM, Hamouda. "Phenotypic and Genotypic Identification of Typical and Atypical Staphylococcus aureus Isolated From Bovine Milk Samples." Open Access Journal of Microbiology & Biotechnology 8, no. 1 (2023): 1–10. http://dx.doi.org/10.23880/oajmb-16000254.

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This study aimed to determine the phenotypic and genotypic identification and differentiation of typical coagulase positive Staphylococcus aureus (CPSA) and atypical coagulase negative Staphylococcus aureus (CNSA) from other Staphylococci isolated form bovine milk. Out of 111 milk samples, 67 Staphylococcus strains were isolated, phenotypically, based on resistance to Acriflavine; Staphylococcus aureus (S. aureus) was detected in 44 isolates of them (30 CPSA strains and 14 CNSA strains). Twenty five from CPSA and 5 from CNSA isolates were positive for slime production on Congo red agar plates.
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Samira Fattah Hamid and Aza Bahadeen Taha. "Mannitol Fermenting Methicillin-Resistant Coagulase Negative Staphylococci Isolated From Diabetic Foot Infections." International Journal of Research in Pharmaceutical Sciences 11, no. 4 (2020): 6095–101. http://dx.doi.org/10.26452/ijrps.v11i4.3281.

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Detection of mannitol fermenting coagulase-negative staphylococci is frequently unnoticed when Staphylococcus aureus is screening in the laboratory. On the other hand, the emergence of coagulase-negative staphylococci as critical human pathogens need dependable methods for the identification of clinically significant coagulase-negative staphylococci to understand the epidemiology of infections caused by these bacteria. The study aimed to identify mannitol fermenting coagulase-negative staphylococci that assumed to be Staphylococcus aureus as they formed yellow colonies on Mannitol Salt agar pl
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Bulajić, Snežana, Zora Mijačević, Tijana Ledina, and Bojan Golić. "Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci." Acta Veterinaria 65, no. 4 (2015): 518–37. http://dx.doi.org/10.1515/acve-2015-0044.

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AbstractSjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheep’s milk in the southern part of Serbia - Sjenica Pester plateau.The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated.During the monitoring period of the cheese-making process,
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El-Diasty, Mohamed, Hager Talaat, Samar Atwa, Elzahraa Elbaz, and Mohamed Eissa. "Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows." September2019 20, no. 3 (2019): 35–39. http://dx.doi.org/10.35943/mvmj.2019.23.207.

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Objective: To study the prevalence of Coagulase-negative Staphylococci in clinical and subclinical mastitis in dairy cows. Design: Case-control study. Animals: 415 lactating Holstein cows. Procedures: A total of 896 quarter milk samples were collected aseptically from 415 dairy cows for bacteriological examination (mastitic cows, n=50; healthy cows, n=174). Identification of different bacterial isolates followed by antibiotic sensitivity test for Coagulase-negative Staphylococci (CNS) was carried out, using disc-diffusion method against 13 different antibiotics. Results: 224 out of 415 cows (5
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Speers, David J., Thomas R. Olma, and Gwendolyn L. Gilbert. "Evaluation of Four Methods for Rapid Identification of Staphylococcus aureus from Blood Cultures." Journal of Clinical Microbiology 36, no. 4 (1998): 1032–34. http://dx.doi.org/10.1128/jcm.36.4.1032-1034.1998.

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The identification of Staphylococcus aureus directly from blood cultures is clinically relevant, but it requires a test that is both rapid and reliable. Previously, biochemical, immunological, tube coagulase, and thermostable-endonuclease methods have shown variable sensitivity and specificity. Testing directly from blood culture broth has not been described for the latex kit Staphaurex Plus (Murex Diagnostics Ltd.), and the modified conventional tests have not been used with the newer, continuously monitored blood culture systems. In addition, the commercial RAPIDEC staph kit (bioMerieux Vite
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Faiq, Shaween Omed, and Sherko Ali Omer. "Clinical and Resistance Pattern of Coagulase Negative Staphylococci." Advanced Medical Journal 8, no. 1 (2023): 40–47. http://dx.doi.org/10.56056/amj.2023.196.

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Background and Objectives: Coagulase-negative staphylococci are commensal bacteria inhabiting human. Isolation of these bacteria from healthcare-associated and community infections is increasingly observed with resistance response to many antibiotics. The study aimed to identify the clinical and antimicrobial resistance pattern of coagulase-negative staphylococci isolated from various healthcare-associated and community-acquired infections. Methods: This cross-sectional study was conducted in Shar Teaching Hospital and San Clinical Laboratory for a period of one year starting from 1st of Novem
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CHANG, TSUNG C., and SU H. HUANG. "Evaluation of Coagulase Activity and Protein A Production for the Identification of Staphylococcus aureus." Journal of Food Protection 58, no. 8 (1995): 858–62. http://dx.doi.org/10.4315/0362-028x-58.8.858.

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The activities of coagulase and thermostable nuclease (TNase) and the production of protein A were studied in 338 bacterial strains. These included 213 isolates of Staphylococcus aureus to determine which characteristic was most specific for the identification of S. aureus. The evaluation of different protocols for interpretation of coagulase results was also undertaken. Protein A was analyzed by a sandwich enzyme-linked immunosorbent assay using microtiter plates coated with antiprotein A antibodies. Coagulase activities were determined according to the criteria recommended by Association of
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Cox, Hollis Utah, Susan S. Newman, Alma F. Roy, Johnny D. Hoskins, and Carol S. Foil. "Comparison of coagulase test methods for identification of Staphylococcus intermedius from dogs." American Journal of Veterinary Research 46, no. 7 (1985): 1522–25. https://doi.org/10.2460/ajvr.1985.46.07.1522.

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SUMMARY Staphylococcus intermedius isolates from dogs were tested for coagulase activity by 6 commercial methods and by conventional methods, using rabbit and dog plasma. When compared with the conventional tube method using rabbit plasma, none of the 6 commercial methods was suitable for identification of S intermedius, although the 6 tests performed well using strains of S aureus. Use of rabbit plasma identified more S intermedius isolates than did use of dog plasma.
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SAVINI, VINCENZO, MAJA KOSECKA, ROBERTA MARROLLO, EDOARDO CARRETTO та JACEK MIĘDZOBRODZKI. "CAMP Test Detected Staphylococcus delphini ATCC49172 β-haemolysin Production". Polish Journal of Microbiology 62, № 4 (2013): 465–66. http://dx.doi.org/10.33073/pjm-2013-065.

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Through a CAMP test, we first observed a Staphylococcus delphini strain (ATCC 49172) to release beta-haemolysin. Production of the latter in this coagulase-positive species of the 'Staphylococcus intermedius Group', in fact, has been labeled to be undetermined, thus far. Of course, a wider number of strains have to be investigated in order to define whether this property is constitutive (like in Staphylococcus (pseud)intermedius), or strain-dependent (like in Staphylococcus aureus), and which clinical impact it has; nevertheless, we can state that S. delphini ATCC 49172 indeed produces this to
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Mohamed, El-Diasty, Talaat Hager, Atwa Samar, Elbaz Elzahraa, and Eissa Mohamed. "Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows." Mansoura Veterinary Medical Journal 20, no. 3 (2019): 35–39. https://doi.org/10.35943/mvmj.2019.23.207.

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<strong>Abstract</strong> <strong><em>Objective:</em></strong> To study the prevalence of Coagulase-negative Staphylococci in clinical and subclinical mastitis in dairy cows. <strong><em>Design:</em></strong> Case-control study. <strong><em>Animals:</em></strong>&nbsp; 415 lactating Holstein cows. <strong><em>Procedures:</em></strong>&nbsp; A total of 896 quarter milk samples were collected aseptically from 415 dairy cows for bacteriological examination (mastitic cows, n=50; healthy cows, n=174). Identification of different bacterial isolates followed by antibiotic sensitivity test for Coagula
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Kaplan, N. M. "Use of thermonuclease testing to identify Staphylococcus aureus by direct examination of blood cultures." Eastern Mediterranean Health Journal 9, no. 1-2 (2003): 185–90. http://dx.doi.org/10.26719/2003.9.1-2.185.

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Blood cultures submitted to the Clinical Microbiology Laboratory, Queen Alia Military Hospital, Amman during 1999-2001 were examined to evaluate thermonuclease testing for identifying Staphylococcus aureus in blood culture broths growing gram-positive cocci. Of 170 cultures studied, 129 yielded gram-positive staphylococci and 41 yielded other gram-positive cocci. Toluidine blue-deoxynucleic acid agar plates were used to test for thermonuclease activity. St and ard tube coagulase tests were performed on the isolates. Direct detection of thermonuclease activity in 76 blood culture broths contain
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Ribeiro, Marlice Teixeira, Wanderlei Ferreira de Sá, Leda Aroni Melchíades, Vânia Maria de Oliviera Veiga, and Iveraldo Santos Dutra. "CARACTERIZAÇÃO DO STAPHYLOCOCCUS COAGULASE NEGATIVO ISOLADOS DE QUARTOS MAMÁRIOS DE VACAS COM MASTITE SUBCLÍNICA." Ciência Rural 24, no. 2 (1994): 329–32. http://dx.doi.org/10.1590/s0103-84781994000200020.

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RESUMO O presente trabalho foi desenvolvido na Zona da Mata - MG, durante três meses. De quatro rebanhos utilizados, trabalhou-se com 16 animais mestiços e 19 holandeses. O objetivo foi caracterizar Staphylococcus coagulase negativos, isolados de quartos mamários de vacas, que inicialmente reagiam à prova do "Califórnia Mastitis Test" (CMT), em quatro testes consecutivos, a intervalo de uma semana. A caracterização das 116 amostras encontradas, correspondentes a Staphylococcus coagulase negativo, foram: S. epidermidis (71,55%), S. hyicus subsp. chromogenes (6,03%), S. simulans (6,03%), S. warn
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Varatanovic, N., A. Katica, T. Mutevelic, B. Cengic, N. Mlaco, and E. Hamzic. "Research of mastitis prevalence at heifers in farm breeding." Biotehnologija u stocarstvu 25, no. 1-2 (2009): 73–80. http://dx.doi.org/10.2298/bah0902073v.

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With 268 heifers in farm breeding, udder examination has been performed for clinical mastitis during 14 days after calving. From each quarter we took secretion samples and performed bacteriological analysis. From total number of heifers, 56 of them had shown clinical signs of inflammation, and 12 were bacteriologicaly positive. By bacteriological analysis mastitis pathogens had been found, even in 14 heifers without inflammation signs, at least until the end of test period. From the total number of tested heifers in 3,35% of cases Streptococcus agalactiae have been found, 4,85% coagulase posit
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Sandhya, Narayanan, S. Deepa, and P.L. Soundarya. "Comparative Evaluation of Vancomycin Disc Diffusion Method & Vancomycin Screen Agar with Vancomycin E-Test in the Detection of Clinically Significant MRCoNS." International Journal of Pharmaceutical and Clinical Research 14, no. 5 (2022): 578–85. https://doi.org/10.5281/zenodo.13829007.

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<strong>Background and Objectives:</strong>&nbsp;<em>Coagulase negative Staphylococci&nbsp;</em>(CoNS) are colonizers of the skin, anterior nares, and ear canals of human beings. They have long been considered as non-pathogenic but as a result of the combination of increased use of intravascular devices and increase in the number of hospitalized immunocompromised patients, they have become one of the important nosocomial pathogens.&nbsp; One of the characteristics of CoNS is their resistance to multiple antimicrobial agents. Some recent reports have pointed out high level of methicillin resist
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Kolář, Milan, Jan Bardoň, Vojtěch Hanulík, Pavel Sauer, Vladimír Babák, and Jarmila Schlegelová. "Resistance to Methicillin in Coagulase-negative Staphylococci and Its Detection." Acta Veterinaria Brno 79, no. 2 (2010): 261–67. http://dx.doi.org/10.2754/avb201079020261.

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Resistance of staphylococci to methicillin is important especially in the case ofStaphylococcus aureusisolates. Its impact in veterinary medicine is not exactly specified in coagulase-negative staphylococci; however, these staphylococci may represent an important reservoir of resistance genes. The study aimed at detecting resistance to methicillin in coagulase-negative staphylococci from raw materials and foodstuffs of animal origin and assessing the tests frequently used to determine this resistance. Coagulase-negative staphylococci (198 isolates of 12 species) were tested. Resistance to meth
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Hsueh, Po-Ren, Lee-Jene Teng, Pan-Chyr Yang, et al. "Dissemination of Two Methicillin-Resistant Staphylococcus aureus Clones Exhibiting Negative Staphylase Reactions in Intensive Care Units." Journal of Clinical Microbiology 37, no. 3 (1999): 504–9. http://dx.doi.org/10.1128/jcm.37.3.504-509.1999.

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From December 1997 to March 1998, 25 methicillin-resistantStaphylococcus aureus (MRSA) isolates exhibiting negative Staphylase (Oxoid Ltd., Basingstoke, England) reactions were identified from various clinical specimens from 13 patients in six intensive care units (ICUs) or in wards following a stay in an ICU at the National Taiwan University Hospital. The characteristics of these isolates have not been previously noted in other MRSA isolates from this hospital. Colonies of all these isolates were grown on Trypticase soy agar supplemented with 5% sheep blood and were nonhemolytic and unpigment
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