Academic literature on the topic 'Coffee anthracnose'

Trichoderma of coffee roots from Alor: Morphological characteristic and in vitro efficacy to inhibit Colletotrichum, causing Anthracnose Trichoderma is a fungus capable of intimate associations with plant root systems including on coffee plants. This aim of study is to determine the characteristics of Trichoderma morphospecies from coffee roots of Alor origin, East Nusa Tenggara (NTT) and its ability to inhibit the growth of Colletotrichum causing anthracnose disease in-vitro. Root samples of healthy coffee plants were taken from the location of coffee plantations in Alor District, NTT. Isolation of Trichoderma fungi from coffee roots was done by incubating the sterilized coffee roots in a layer of moist filter paper in a Petri dish for seven days. Identification of Trichoderma by observing the characteristics of the colony on the medium of potato dextrose agar (PDA) and microscopic media using microcultures (slide culture). Inhibition of Trichoderma fungi against Colletotrichum was tested by multiple culture methods on PDA media. The results of root incubation in humid conditions showed that there was four morphospecies of Trichoderma fungi, each of which had different characteristic specifications. In vitro antagonism in test on PDA medium, the first three morphospecies against Colletotrichum showed that each Trichoderma could inhibit 70.2%, 65.8%, and 63.3%, respectively, five days after inoculation. This data shows that Trichoderma isolated from coffee roots from Alor has the potential to suppress the growth of anthracnose pathogens.

In Brazil, dieback and necrosis of leaves and berries of coffee trees (Coffea arabica and C. canephora) are common symptoms of anthracnose disease caused by Colletotrichum gloeosporioides (Penz.) Sacc. In April 2010, these symptoms were observed in 100% of the plants from different coffee plantations in the Brazilian states of Espírito Santo and Bahia. Ten isolates were obtained from symptomatic leaves and berries from these areas. Of the 10 isolates, one had distinct conidial morphology with hyaline and ellipsoid conidia measuring 10 to 16 × 5.0 to 7.5 μm and melanized irregular or spatulated-shaped appressoria measuring 7.5 to 11.0 × 5.5 to 8.5 μm, formed either solitary or concatenated, which concurred with the conidia description of Colletotrichum boninense. In order to confirm the identity of this isolate, the internal transcribed spacer (ITS) rRNA region and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene were sequenced (GenBank Accession Nos. JF683320 and JF331654, respectively) and compared to sequences from a database of C. boninense, confirming that the isolate was definitely C. boninense sensu lato, since it was exactly identical to other sequences in a large clade of isolates. To verify the pathogenicity of C. boninense in coffee and to compare the symptoms with those caused by C. gloeosporioides, leaves and berries were inoculated with the isolate of C. boninense and one representative isolate of C. gloeosporioides, both expressing the GFP (green fluorescent protein) gene. The isolates were grown for 7 days on potato dextrose agar and a conidial suspension (106 conidia × ml–1) was used to inoculate the organs, wounded and non-wounded, at different stages of development. In non-wounded organs, the conidial suspension was inoculated on the surface, and in leaves and berries used as control, the suspensions were substituted for sterile water. Leaves and berries were wounded with a sterilized needle and inoculated with 20 and 10 μl of the conidial suspension, respectively. Inoculated materials were incubated at 25°C and 100% relative humidity. The experiment was performed twice and evaluated daily for a week. No symptoms were observed on the control and non-wounded organs, while wounded organs exhibited typical anthracnose symptoms for both species. In berries, C. gloeosporioides consistently caused more severe symptoms at a faster rate than C. boninense. Both fungi caused necrosis in young but not old leaves. Typical acervuli were observed on the lesions and the fungus was successfully recovered from the inoculated tissues, which was confirmed by fluorescence microscopy, fulfilling Koch's Postulates. C. boninense has been identified as a pathogen causing anthracnose in a range of hosts worldwide. However, in Brazil, it has only been reported in pepper (Capsicum annuum) (3), passion fruit (Passiflora) (4), Hippeastrum (1) and in the medicinal plant Maytenus ilicifolia (2). To our knowledge, this is the first report of C. boninense associated with anthracnose of coffee trees in Brazil. Since the symptoms are similar to those caused by C. gloeosporioides, it can be stated that both species are associated with this disease in commercial coffee plantations in Brazil. Therefore, control strategies should consider the occurrence of C. boninense. References: (1) D. F. Farr et al. Mycol. Res. 110:1395, 2006. (2) S. A. Pileggi et al. Can. J. Microbiol. 55:1076, 2009. (3) H. J. Tozze et al. Plant Dis. 93:106, 2009. (4) H. J. Tozze et al. Australas. Plant Dis. Notes 5:70, 2010.

Colletotrichum siamense causes fruit or foliar disease called anthracnose on a variety of plant hosts such as vegetables, fruits, ornamental plants, and others, including chili pepper, apple, American cranberry, mango, orange, papaya, guava, rubber plant, jasmine, coffee berry, and tea plants. Here, we report the first Illumina-sequenced draft genome assembly of C. siamense strain ICMP 18578 and its annotation. This genome sequence provides a unique resource that will be useful for future research on the evolution of Colletotrichum spp. and improvement of anthracnose management strategies.

This study aimed to verify the viability of propagation material from coffee plants descended from germplasm susceptible to blister spot disease as well as its susceptibility to Colletotrichum sp. relative to commercial coffee cultivars. In the first experiment, fruits were harvested from plants with and without symptoms of blister spot and sowed in trays containing a commercial sterilized substrate. The percentages of germinated seeds, viable plantlets and seedlings were evaluated. In diseased tissues, pathogens were isolated and identified though a pathogenicity test. In the second experiment, ten commercial cultivars and one cultivar originating from plants with blister spot were inoculated with the pathogens to assess the severity of anthracnose. Significant differences were not observed with respect to seed germination. However, the viability of plantlets and seedlings was reduced in the cultivar originating from plants with blister spot (Genotype Originated from Diseased Plants-GODP). These plants showed characteristic symptoms of blister spot, including necrosis in the leaves and hypocotyls, wilting and death. In the necrotic lesions, we observed characteristic sporulation of Colletotrichum sp. The cultivar most susceptible to anthracnose in cotyledonary leaves was Catuaí Vermelho (GODP), which presented the highest area under the disease progress curve (AUDPC). In conclusion, the viability of propagation material from coffee plants that had descended from plants with symptoms of blister spot (GODP) was reduced compared with plants from other genotypes, although seed germination was not affected. Moreover, GODP species are more susceptible to Anthracnose on the cotyledonary leaves relative to the other analyzed commercial cultivars. This work is the first to report on different symptoms exhibited by seedlings originating from the seeds of plants with symptoms of blister spot.

Coffee agroforestry systems could reconcile agricultural and environmental objectives. While pests and diseases can reduce yield, their interactions with shade and nutrition have been rarely researched, and are particularly lacking in perennial systems. We hypothesized that intermediate shade levels could reduce coffee pests while excess shade could favor fungal diseases. We hypothesized that organic rather than mineral fertilization would better synchronize with nutrient uptake and higher nutrient inputs would be associated with reduced pest and disease damage due to higher plant vigor, yet effects would be less obvious in shaded plots as coffee growth would be light-limited. Using three-year-old trees of Coffea canephora var. Robusta (robusta coffee) in the Ecuadorian Amazon, we compared a full-sun system with four shading methods creating different shade levels: (1) Myroxylon balsamum; (2) Inga edulis; (3) Erythrina spp.; or, (4) Erythrina spp. plus Myroxylon balsamum. Conventional farming at either (1) moderate or (2) intensified input and organic farming at (3) low or (4) intensified input were compared in a split-plot design with shade as the main plot factor and farming practice as the sub-plot factor. The infestation of the following pests and disease incidences were evaluated monthly during the dry season: brown twig beetle (Xylosandrus morigerus), coffee leaf miner (Leucoptera coffeella), coffee berry borer (Hypothenemus hampei), anthracnose disease (Colletotrichum spp.), thread blight (Pellicularia koleroga), and cercospora leaf spot (Cercospora coffeicola). Coffee berry borer and brown twig beetle infestation were both reduced by 7% in intensified organic treatments compared to intensified conventional treatments. Colonization of coffee berry borer holes in coffee berries by the entomopathogenic fungus Beauveria bassiana was also assessed. Brown twig beetle infestation was significantly higher under full sun than under Inga edulis, yet no other shade effects were detected. We demonstrate for the first time how intensified input use might promote pest populations and thus ultimately lead to robusta coffee yield losses.

In the current study, we used bio-organic product POLYFA-TN3 including beneficial indigenous microorganisms isolated in Tay Nguyen to improve soil quality, prevent plant diseases, and increase the yield of black pepper and coffee in Dak Lak province. Experimental results showed the efficiency of using POLYFA-TN3 on improvement of soil quality. Almost parameters of soil were increased after using POLYFA-TN3, of which soluble N, K, and P were significantly increased. Using POLYFA-TN3 also reduced plant diseases of black pepper and coffee such as yellow leaf, slow wilt decline, anthracnose, Phytophthora root rot, coffee rust. Using 2.0 kg POLYFA-TN3/tree post showed that plant diseases dropped to 0.5 - 7.53 % for black pepper, and 0 % for coffee. In addition, the yield of black pepper and coffee was significantly increased, with 35.2 % and 31.1 %, respectively. Our study indicated that the bio-organic product POLYFA-TN3 as a potential product for improvement of soil quality, crop yield, and prevention of plant diseases.

Fruit rots reduce coffee production worldwide. Eight Colletotrichum species have been reported to cause coffee fruit rots; the most important is C. kahawae, the cause of coffee berry disease (CBD) in Africa. It is unknown whether these fruit rot pathogens can be dispersed by the coffee berry borer (CBB, Hypothenemus hampei) or whether Beauveria bassiana (a natural enemy of CBB) might reduce coffee fruit rots. We identified pathogens causing coffee fruits rots in Puerto Rico and evaluated whether B. bassiana reduced fruit rot and whether CBB could disperse pathogens. A total of 2,333 coffee fruit with CBB damage were collected; of these, 1,197 had visible growth of B. bassiana. C. fructicola, C. siamense, C. theobromicola, and C. tropicale were isolated and identified from the fruit using morphological traits and phylogeny of three nuclear genes. All four species caused internal and external rot after inoculation of healthy green coffee fruit. Coffee fruit treated with B. bassiana had significantly less fruit rot than untreated fruit, suggesting B. bassiana can protect against fruit rot. To test whether B. bassiana had a protective effect, B. bassiana and Colletotrichum were coinoculated on coffee fruit. Fruit inoculated with both B. bassiana and Colletotrichum had significantly less rot than fruit inoculated with Colletotrichum alone. To test if CBBs dispersed the pathogens, CBBs were exposed to Colletotrichum conidia and placed on green fruit, which resulted in fruit rot. This study identifies new pathogens causing coffee fruit rot, shows that C. kahawae is not the only Colletotrichum that attacks green fruits, suggests a role for B. bassiana in disease management and demonstrates CBB can disperse the pathogens.

Colletotrichum asianum is a worldwide plant pathogen causing serious fruit or leaf anthracnose diseases on a variety of plant hosts such as mango, coffee berry, chili, and other potential hosts, and it is distributed widely in Asia, America, Africa, and Oceania. This is the first genome resource available for C. asianum. The draft genome assembly will allow further analysis of species diversity and evolutionary mechanisms, and may serve as a foundation for genetic analysis that leads to greater understanding of interactions between plants and fungal pathogens.