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1

Sugawara, Yo, Hideharu Hagiya, Yukihiro Akeda, et al. "Community spread and acquisition of clinically relevant Escherichia coli harbouring blaNDM among healthy Japanese residents of Yangon, Myanmar." Journal of Antimicrobial Chemotherapy 76, no. 6 (2021): 1448–54. http://dx.doi.org/10.1093/jac/dkab070.

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Abstract Background Carbapenemase-producing Enterobacterales (CPE) are spreading in hospitals, environment and retail foods in Yangon, Myanmar. Objectives To investigate whether CPE colonize healthy individuals living in Yangon and whether clinical-related strains are spreading in the community. Methods CPE was isolated from faecal samples obtained from healthy Japanese residents of Yangon with no history of hospitalization. Isolates were subjected to WGS using short- and long-read sequencers and compared with those previously isolated in Yangon. Results Six Escherichia coli strains harbouring
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2

Fonseca, Erica L., Sergio M. Morgado, Raquel V. Caldart, and Ana Carolina Vicente. "Global Genomic Epidemiology of Escherichia coli (ExPEC) ST38 Lineage Revealed a Virulome Associated with Human Infections." Microorganisms 10, no. 12 (2022): 2482. http://dx.doi.org/10.3390/microorganisms10122482.

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Background: Most of the extraintestinal human infections worldwide are caused by specific extraintestinal pathogenic Escherichia coli (ExPEC) lineages, which also present a zoonotic character. One of these lineages belongs to ST38, a high-risk globally disseminated ExPEC. To get insights on the aspects of the global ST38 epidemiology and evolution as a multidrug-resistant and pathogenic lineage concerning the three axes of the One Health concept (humans, animals, and natural environments), this study performed a global phylogenomic analysis on ST38 genomes. Methods: A phylogenetic reconstructi
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3

Brendecke, Jana, Timo Homeier-Bachmann, Angela Schmitz Ornés, et al. "Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany." Antibiotics 11, no. 10 (2022): 1357. http://dx.doi.org/10.3390/antibiotics11101357.

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Multidrug-resistant (MDR) Enterobacterales, including extended-spectrum β‑lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, not only emerge in healthcare settings but also in other habitats, such as livestock and wildlife. The spread of these pathogens, which often combine resistance with high-level virulence, is a growing problem, as infections have become increasingly difficult to treat. Here, we investigated the occurrence of ESBL-producing E. coli and K. pneumoniae in fecal samples from two black-headed gull colonies breeding on two nature conservation islands in Weste
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4

Soncini, João Gabriel Material, Vanessa Lumi Koga, Bruna Fuga, et al. "Molecular Analysis of Escherichia coli and Correlations Between Phylogroups and Sequence Types from Different Sources." Microorganisms 12, no. 12 (2024): 2645. https://doi.org/10.3390/microorganisms12122645.

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Escherichia coli is a significant pathogen responsible for infections in both humans and livestock, possessing various virulence mechanisms and antimicrobial resistance that make it even more concerning. In this study, several internationally recognized clones of E. coli were identified, such as ST131, ST38, ST648, and ST354, from chicken meat, pork, and human infection samples. Notably, ST131, belonging to phylogroup B2, was the dominant sequence type (ST) in human samples, while ST38, belonging to phylogroup D, was the most prevalent in meat samples. Several antibiotic resistance genes were
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5

Vingino, Alexandria, Marilyn C. Roberts, Michelle Wainstein, et al. "Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem." Antibiotics 10, no. 10 (2021): 1201. http://dx.doi.org/10.3390/antibiotics10101201.

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E. coli was isolated from the Salish Sea (Puget Sound) ecosystem, including samples of marine and fresh water, and wildlife dependent on this environment. E. coli isolates were assessed for phenotypic and genotypic resistance to antibiotics. A total of 305 E. coli isolates was characterized from samples collected from: marine water obtained in four quadrants of the Salish Sea; select locations near beaches; fresh water from streams near marine beaches; and fecal samples from harbor porpoises (Phocoena phocoena), harbor seals (Phoca vitulina), river otters (Lontra canadensis), and English sole
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6

Paramita, Rafika Indah, Erni Juwita Nelwan, Fadilah Fadilah, Editha Renesteen, Nelly Puspandari, and Linda Erlina. "Genome-based characterization of Escherichia coli causing bloodstream infection through next-generation sequencing." PLOS ONE 15, no. 12 (2020): e0244358. http://dx.doi.org/10.1371/journal.pone.0244358.

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Escherichia coli are one of the commonest bacteria causing bloodstream infection (BSI). The aim of the research was to identify the serotypes, MLST (Multi Locus Sequence Type), virulence genes, and antimicrobial resistance of E. coli isolated from bloodstream infection hospitalized patients in Cipto Mangunkusumo National Hospital Jakarta. We used whole genome sequencing methods rather than the conventional one, to characterized the serotypes, MLST (Multi Locus Sequence Type), virulence genes, and antimicrobial resistance (AMR) of E. coli. The composition of E. coli sequence types (ST) was as f
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7

Poirel, Laurent, Sandrine Bernabeu, Nicolas Fortineau, Isabelle Podglajen, Christine Lawrence, and Patrice Nordmann. "Emergence of OXA-48-Producing Escherichia coli Clone ST38 in France." Antimicrobial Agents and Chemotherapy 55, no. 10 (2011): 4937–38. http://dx.doi.org/10.1128/aac.00413-11.

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8

Al Bayssari, Charbel, Abiola Olumuyiwa Olaitan, Fouad Dabboussi, Monzer Hamze, and Jean-Marc Rolain. "Emergence of OXA-48-Producing Escherichia coli Clone ST38 in Fowl." Antimicrobial Agents and Chemotherapy 59, no. 1 (2014): 745–46. http://dx.doi.org/10.1128/aac.03552-14.

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9

GERHOLD, G., M. H. SCHULZE, U. GROSS, and W. BOHNE. "Multilocus sequence typing and CTX-M characterization of ESBL-producing E. coli: a prospective single-centre study in Lower Saxony, Germany." Epidemiology and Infection 144, no. 15 (2016): 3300–3304. http://dx.doi.org/10.1017/s0950268816001412.

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SUMMARYThe increasing prevalence of extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacteria is a serious threat for current healthcare settings. In this study we investigated the molecular epidemiology of ESBL-producing E. coli at the University Medical Center Göttingen in Lower Saxony, Germany. All E. coli isolates with an ESBL phenotype were collected during a 6-month period in 2014. Multilocus sequence typing and CTX-M characterization were performed on 160 isolates. Of the ESBL-producing isolates 95·6% were CTX-M positive. Compared to recent Germany-wide studies, we found CTX
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10

Huang, Lili, Hongye Hu, Chen Xu, et al. "Characterization of NDM-5-Producing Escherichia coli Strains Isolated from Pediatric Patients with Bloodstream Infections in a Chinese Hospital." Genes 14, no. 2 (2023): 520. http://dx.doi.org/10.3390/genes14020520.

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Escherichia coli (E. coli) bloodstream infections (BSIs) are among the most predominant causes of death in infants and children worldwide. NDM-5 (New Delhi Metallo-lactamase-5) is responsible for one of the main mechanisms of carbapenem resistance in E. coli. To analyze the phenotypic and genomic characteristics of NDM-5-producing E. coli from bloodstream infections (BSIs), a total of 114 E. coli strains was collected from a children’s hospital in Jiangsu province, China. Eight blaNDM-5-carrying E. coli strains were identified which were all carbapenem-resistant and carried diverse antimicrobi
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11

Abid, Fatma Ben, Clement K. M. Tsui, Yohei Doi, et al. "Molecular characterization of clinical carbapenem-resistant Enterobacterales from Qatar." European Journal of Clinical Microbiology & Infectious Diseases 40, no. 8 (2021): 1779–85. http://dx.doi.org/10.1007/s10096-021-04185-7.

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AbstractOne hundred forty-nine carbapenem-resistant Enterobacterales from clinical samples obtained between April 2014 and November 2017 were subjected to whole genome sequencing and multi-locus sequence typing. Klebsiella pneumoniae (81, 54.4%) and Escherichia coli (38, 25.5%) were the most common species. Genes encoding metallo-β-lactamases were detected in 68 (45.8%) isolates, and OXA-48-like enzymes in 60 (40.3%). blaNDM-1 (45; 30.2%) and blaOXA-48 (29; 19.5%) were the most frequent. KPC-encoding genes were identified in 5 (3.6%) isolates. Most common sequence types were E. coli ST410 (8;
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12

Markovska, Rumyana, Petya Stankova, Temenuga Stoeva, et al. "Fecal Carriage and Epidemiology of Extended-Spectrum Beta-Lactamase/Carbapenemases Producing Enterobacterales Isolates in Bulgarian Hospitals." Antibiotics 10, no. 6 (2021): 747. http://dx.doi.org/10.3390/antibiotics10060747.

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The gastrointestinal tract is an important reservoir of extended spectrum beta-lactamase (ESBL)/carbapenemase-producing Enterobacterales isolates. This study included patients from two Bulgarian hospitals. Overall, 98 ESBL producers (including 68 Escherichia coli and 20 Klebsiella pneumoniae isolates) were detected among 99 hospitalized patients, 212 patients at admission, and 92 hospital staff in 42.4%, 24.5%, and 4%, respectively. We observed blaCTX-M-15 in 47% of isolates, blaCTX-M-3 in 39% and blaCTX-M-14 in 11%. Three blaCTX-M-15 positive isolates were also blaKPC-2 positive. High transfe
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13

Yasugi, Mayo, Shingo Hatoya, Daisuke Motooka та ін. "Whole-genome analyses of extended-spectrum or AmpC β-lactamase-producing Escherichia coli isolates from companion dogs in Japan". PLOS ONE 16, № 2 (2021): e0246482. http://dx.doi.org/10.1371/journal.pone.0246482.

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The emergence and global spread of extended-spectrum or AmpC β-lactamase (ESBL/AmpC)-producing Enterobacteriaceae in companion animals have led to the hypothesis that companion animals might be reservoirs for cross-species transmission because of their close contact with humans. However, current knowledge in this field is limited; therefore, the role of companion animals in cross-species transmission remains to be elucidated. Herein, we studied ESBL/AmpC-producing Enterobacteriaceae, Escherichia coli in particular, isolated from extraintestinal sites and feces of companion dogs. Whole-genome s
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Guenther, Sebastian, Torsten Semmler, Annegret Stubbe, Michael Stubbe, Lothar H. Wieler, and Katharina Schaufler. "Chromosomally encoded ESBL genes in Escherichia coli of ST38 from Mongolian wild birds." Journal of Antimicrobial Chemotherapy 72, no. 5 (2017): 1310–13. http://dx.doi.org/10.1093/jac/dkx006.

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15

Turton, Jane F., Michel Doumith, Katie L. Hopkins, Claire Perry, Daniele Meunier, and Neil Woodford. "Clonal expansion of Escherichia coli ST38 carrying a chromosomally integrated OXA-48 carbapenemase gene." Journal of Medical Microbiology 65, no. 6 (2016): 538–46. http://dx.doi.org/10.1099/jmm.0.000248.

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16

van der Bij, Akke K., Gisele Peirano, Wil H. F. Goessens, Eric R. van der Vorm, M. van Westreenen та Johann D. D. Pitout. "Clinical and Molecular Characteristics of Extended-Spectrum- β-Lactamase-Producing Escherichia coli Causing Bacteremia in the Rotterdam Area, Netherlands". Antimicrobial Agents and Chemotherapy 55, № 7 (2011): 3576–78. http://dx.doi.org/10.1128/aac.00074-11.

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ABSTRACTWe investigated the clinical and molecular characteristics of bacteremia caused by extended-spectrum-β-lactamase (ESBL)-producingEscherichia coliover a 2-year period (2008 to 2009) in the Rotterdam region (including 1 teaching hospital and 2 community hospitals) of Netherlands. The majority of patients presented with community onset urinary and intra-abdominal infections, with an increase in prevalence during 2009. The majority ofE. coliisolates produced CTX-M-15, and 4 sequence types (ST38, ST131, ST405, and ST648) predominated. There were significant differences in clinical and molec
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17

Park, Ji-Hyun, Kyung-Seon Bae, Jihyun Kang, Jeong-Ki Yoon, and Soo-Hyung Lee. "Comprehensive Assessment of Multidrug-Resistant and Extraintestinal Pathogenic Escherichia coli in Wastewater Treatment Plant Effluents." Microorganisms 12, no. 6 (2024): 1119. http://dx.doi.org/10.3390/microorganisms12061119.

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Multidrug-resistant (MDR) Escherichia coli poses a significant threat to public health, contributing to elevated rates of morbidity, mortality, and economic burden. This study focused on investigating the antibiotic resistance profiles, resistance and virulence gene distributions, biofilm formation capabilities, and sequence types of E. coli strains resistant to six or more antibiotic classes. Among 918 strains isolated from 33 wastewater treatment plants (WWTPs), 53.6% (492/918) demonstrated resistance, 32.5% (298/918) were MDR, and over 8% (74/918) were resistant to six or more antibiotic cl
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18

Yamaji, Reina, Julia Rubin, Cindy Friedman, Patrick McDermott, Melody Hung-Fan, and Lee Riley. "Retail Meat as a Potential Transmission Source of Community-Acquired Urinary Tract Infection." Open Forum Infectious Diseases 4, suppl_1 (2017): S25—S26. http://dx.doi.org/10.1093/ofid/ofx162.065.

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Abstract Background Escherichia coli causes approximately 80% of community-acquired UTI (CA-UTI), but the sources of these uropathogenic E. coli infections are not well established. Recent studies have suggested that food, especially poultry, may serve as a source of UPEC. Here we prospectively examined E. coli isolates from patients with CA-UTI and retail meat concurrently available from the same geographic region to determine the frequency of shared genotypes. Methods Between September 2016 and May 2017, we collected urine samples from patients with UTI examined at a university-affiliated he
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19

Zhang, Yanjun, Hairu Wang, Yanfang Li, Yabin Hou, and Chonghua Hao. "Drug susceptibility and molecular epidemiology of Escherichia coli in bloodstream infections in Shanxi, China." PeerJ 9 (October 25, 2021): e12371. http://dx.doi.org/10.7717/peerj.12371.

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Objectives We carried out a retrospective study to investigate the drug susceptibility and genetic relationship of clinical Escherichia coli isolates from patients with BSIs in Shanxi, China. Methods E. coli isolates causing BSIs were consecutively collected from June 2019 to March 2020. Antimicrobial susceptibility testing was performed by broth microdilution method. PCR was used to detect antimicrobial resistance genes coding for extended-spectrum β-lactamases (ESBLs), phylogenetic groups and seven housekeeping genes of E. coli. Results A total of 76 E. coli were collected. Antimicrobial sus
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20

Truong, Duong Thi Quy, Yaovi Mahuton Gildas Hounmanou, Son Thi Thanh Dang, et al. "Genetic Comparison of ESBL-Producing Escherichia coli from Workers and Pigs at Vietnamese Pig Farms." Antibiotics 10, no. 10 (2021): 1165. http://dx.doi.org/10.3390/antibiotics10101165.

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We analyzed and compared genomes of Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli from pigs and pig farm workers at 116 farms in Vietnam. Analyses revealed the presence of blaCTX-M-55, blaCTX-M-27, blaCTX-M-15, blaCTX-M-14, blaCTX-M-3, blaCTX-M-65, blaCTX-M-24, blaDHA-1, and blaCMY2 in both hosts. Most strains from pigs contained quinolones (qnr) and colistin resistance genes (mcr-1 and mcr-3). Isolates predominantly harbored more than one plasmid replicon and some harbored plasmid replicons on the same contigs as the ESBL genes. Five strains from farm workers of ST38 (2),
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Sato, Toyotaka, Shin-ichi Yokota, Tooru Tachibana та ін. "Isolation of Human Lineage, Fluoroquinolone-Resistant and Extended-β-Lactamase-Producing Escherichia coli Isolates from Companion Animals in Japan". Antibiotics 10, № 12 (2021): 1463. http://dx.doi.org/10.3390/antibiotics10121463.

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An increase in human and veterinary fluoroquinolone-resistant Escherichia coli is a global concern. In this study, we isolated fluoroquinolone-resistant E. coli isolates from companion animals and characterized them using molecular epidemiological analysis, multiplex polymerase chain reaction to detect E. coli ST131 and CTX-M type extended-spectrum β-lactamases (ESBL), and multi-locus sequence typing analysis. Using plain-CHROMagar ECC, 101 E. coli isolates were isolated from 34 rectal swabs of dogs and cats. The prevalence of resistance to fluoroquinolone and cefotaxime was 27.7% and 24.8%, r
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Kocsis, Béla, Dániel Gulyás, and Dóra Szabó. "Emergence and Dissemination of Extraintestinal Pathogenic High-Risk International Clones of Escherichia coli." Life 12, no. 12 (2022): 2077. http://dx.doi.org/10.3390/life12122077.

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Multiresistant Escherichia coli has been disseminated worldwide, and it is one of the major causative agents of nosocomial infections. E. coli has a remarkable and complex genomic plasticity for taking up and accumulating genetic elements; thus, multiresistant high-risk clones can evolve. In this review, we summarise all available data about internationally disseminated extraintestinal pathogenic high-risk E. coli clones based on whole-genome sequence (WGS) data and confirmed outbreaks. Based on genetic markers, E. coli is clustered into eight phylogenetic groups. Nowadays, the E. coli ST131 c
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Abril, Deisy, Ingrid Gisell Bustos Moya, Ricaurte Alejandro Marquez-Ortiz, et al. "First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent." Antibiotics 8, no. 4 (2019): 222. http://dx.doi.org/10.3390/antibiotics8040222.

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The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged
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Schmitt, Kira, Stefan P. Kuster, Katrin Zurfluh, et al. "Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface." Antibiotics 10, no. 2 (2021): 171. http://dx.doi.org/10.3390/antibiotics10020171.

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Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in a high-risk environment in a companion animal clinic and two animal patients’ households. In an intensive care unit (ICU), rectal swabs from 74 dogs and cats, 74 hand swabs from staff and 298 swabs from surfaces were analyzed for ESBL-E. Seventeen hospitalized patients (23%) and ten (3%) surfaces in the ICU tested ESBL-E positive. Transmission chains for Klebsiella pneumoniae ST307 blaCTX-M-15 and Escherichia coli ST38 blaCT
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Giufrè, Maria, Giulia Errico, Monica Monaco, et al. "Whole Genome Sequencing and Molecular Analysis of Carbapenemase-Producing Escherichia coli from Intestinal Carriage in Elderly Inpatients." Microorganisms 10, no. 8 (2022): 1561. http://dx.doi.org/10.3390/microorganisms10081561.

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The spread of carbapenemase-producing (CP) Enterobacterales is currently a worldwide concern, especially in the elderly. Twelve CP-E. coli isolated from rectal swabs of colonized inpatients aged ≥65 years from four hospitals in two Italian cities (Milan and Rome) were analyzed by whole genome sequencing (WGS) to obtain multi-locus sequence typing (MLST), identification of carbapenemase-encoding genes, resistome, plasmid content, and virulence genes. MLST analysis showed the presence of 10 unrelated lineages: ST410 (three isolates from three different hospitals in two cities) and ST12, ST38, ST
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Aldaz, Nabila, Karen Loaiza, César Marcelo Larrea-Álvarez, Miroslava Anna Šefcová, and Marco Larrea-Álvarez. "In Silico Detection of Virulence Genes in Whole-Genome Sequences of Extra-Intestinal Pathogenic Escherichia coli (ExPEC) Documented in Countries of the Andean Community." Current Issues in Molecular Biology 47, no. 3 (2025): 169. https://doi.org/10.3390/cimb47030169.

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E. coli pathotypes, which cause extra-intestinal infections, pose significant public health challenges, emphasizing the need for virulence gene surveillance to understand their dynamics. Key virulence genes have been identified in E. coli from Andean community countries, predominantly linked to human and animal sources. However, detailed data on virulence profiles from environmental and food sources remain limited. This study utilized an in silico approach to analyze 2402 whole-genome sequences from EnteroBase, known for associations with antimicrobial resistance genes. Of the isolates, 30% we
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Mukerji, Shewli, Marc Stegger, Alec Vincent Truswell, et al. "Resistance to critically important antimicrobials in Australian silver gulls (Chroicocephalus novaehollandiae) and evidence of anthropogenic origins." Journal of Antimicrobial Chemotherapy 74, no. 9 (2019): 2566–74. http://dx.doi.org/10.1093/jac/dkz242.

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Abstract Objectives Antimicrobial resistance (AMR) to critically important antimicrobials (CIAs) amongst Gram-negative bacteria can feasibly be transferred amongst wildlife, humans and domestic animals. This study investigated the ecology, epidemiology and origins of CIA-resistant Escherichia coli carried by Australian silver gulls (Chroicocephalus novaehollandiae), a gregarious avian wildlife species that is a common inhabitant of coastal areas with high levels of human contact. Methods Sampling locations were widely dispersed around the perimeter of the Australian continent, with sites separ
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28

Chenouf, Nadia Safia, Chafik Redha Messaï, Isabel Carvalho, et al. "Serogrouping and Molecular Characterization of ESBL-Producing Avian Pathogenic Escherichia coli from Broilers and Turkeys with Colibacillosis in Algeria." Antibiotics 14, no. 4 (2025): 356. https://doi.org/10.3390/antibiotics14040356.

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Avian colibacillosis caused by avian pathogenic Escherichia coli (APEC) strains is a bacterial disease responsible for enormous economic losses in the poultry industry, due to high mortality rates in farms, antibiotic therapy costs, and seizures at slaughterhouses. The aim of this study was to characterize the serogroups and molecular features of extended spectrum β-lactamase (ESBL)-producing APEC isolates recovered from 248 liver samples of 215 broilers and 33 turkeys with colibacillosis lesions in northeast Algeria. For this, microbiological tests were carried out, according to the recommend
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Hertz, Frederik Boëtius, Jesper Boye Nielsen, Kristian Schønning, et al. "“Population structure of Drug-Susceptible, -Resistant and ESBL-producing Escherichia coli from Community-Acquired Urinary Tract Infections”." BMC Microbiology 16, no. 1 (2016): 63. https://doi.org/10.1186/s12866-016-0681-z.

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<strong>Background: </strong> <i>Escherichia coli</i> is the most common cause of urinary tract infection (UTI). The pathogenic isolates are becoming increasingly resistant to antibiotics; with a worldwide dissemination of resistant sequence types (ST). We characterized three different uropathogenic <i>E. coli</i> populations, from non-hospitalized patients to describe the genetic kinship between resistant and susceptible isolates. We studied the populations by use of multi-locus sequence typing (MLST) and abbreviated-multi locus variable number of tandem repeat analysis (a-MLVA). Urine sample
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Cai, Jia Chang, Rong Zhang, Yan Yan Hu, Hong Wei Zhou, and Gong-Xiang Chen. "Emergence of Escherichia coli Sequence Type 131 Isolates Producing KPC-2 Carbapenemase in China." Antimicrobial Agents and Chemotherapy 58, no. 2 (2013): 1146–52. http://dx.doi.org/10.1128/aac.00912-13.

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ABSTRACTTwenty-two KPC-2-producingEscherichia coliisolates were obtained from three hospitals in Hangzhou, China, from 2007 to 2011. One isolate, with OmpC porin deficiency, exhibited high-level carbapenem resistance. Pulsed-field gel electrophoresis showed that few isolates were indistinguishable or closely related. Multilocus sequence typing indicated that sequence type 131 (ST131) was the predominant type (9 isolates, 40.9%), followed by ST648 (5 isolates), ST405 (2 isolates), ST38 (2 isolates), and 4 single STs, ST69, ST2003, ST2179, and ST744. Phylogenetic analysis indicated that 9 group
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REHMAN, MUHAMMAD ATTIQ, HEIDI REMPEL, CATHERINE D. CARRILLO, et al. "Virulence Genotype and Phenotype of Multiple Antimicrobial-Resistant Escherichia coli Isolates from Broilers Assessed from a “One-Health” Perspective." Journal of Food Protection 85, no. 2 (2021): 336–54. http://dx.doi.org/10.4315/jfp-21-273.

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ABSTRACT Extraintestinal pathogenic Escherichia coli (ExPEC) include several serotypes that have been associated with colibacillosis in poultry and with urinary tract infections (UTIs) and newborn meningitis in humans. In this study, 57 antimicrobial-resistant E. coli from apparently healthy broiler chickens were characterized for their health and safety risks. These isolates belonged to 12 serotypes, and isolates of the same serotype were clonal based on single nucleotide variant analysis. Most of the isolates harbored plasmids; IncC and IncFIA were frequently detected. The majority of the re
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Gashaw, Mulatu, Esayas Kebede Gudina, Guenter Froeschl, et al. "Resistome and Phylogenomics of Escherichia coli Strains Obtained from Diverse Sources in Jimma, Ethiopia." Antibiotics 14, no. 7 (2025): 706. https://doi.org/10.3390/antibiotics14070706.

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Introduction: In recent years, antimicrobial resistance (AMR) rates have increased significantly in bacterial pathogens, particularly extended beta-lactam resistance. This study aimed to investigate resistome and phylogenomics of Escherichia coli (E. coli) strains isolated from various sources in Jimma, Ethiopia. Methods: Phenotypic antibiotic resistance patterns of E. coli isolates were determined using automated Kirby–Bauer disc diffusion and minimum inhibitory concentration (MIC). Isolates exhibiting phenotypic resistance to beta-lactam antibiotics were further analyzed with a DNA microarra
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Grünzweil, Olivia M., Lauren Palmer, Adriana Cabal та ін. "Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals". International Journal of Molecular Sciences 22, № 11 (2021): 5905. http://dx.doi.org/10.3390/ijms22115905.

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Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) w
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Nesporova, Kristina, Adam Valcek, Costas Papagiannitsis, et al. "Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries." Microorganisms 9, no. 4 (2021): 866. http://dx.doi.org/10.3390/microorganisms9040866.

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Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms. A total of 62 Escherichia coli and 22 Klebsiella pneumoniae isolates were obtained and representative isolates were subjected to whole-genome sequencing. Relatively high prevalence of phylogenetic group D and F was observed in E. coli isolates and several zoonotic sequence types (STs) including ST4
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Febrianti, Tati, Nelly Puspandasari, Dwi Febriyana, Tom Weaver, and Anis Karuniawati. "Genotypic pattern of fluoroquinolone resistance among extended-spectrum beta-lactamase-producing <em>Escherichia coli</em>." Medical Journal of Indonesia 33, no. 4 (2025): 213–24. https://doi.org/10.13181/mji.oa.247544.

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BACKGROUND Fluoroquinolone (FQ) is one of the therapeutic options for treating extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) infection, but its use could increase the resistance level of ESBL-Ec. This study aimed to analyze the resistant genes responsible for ESBL production and FQ resistance among the ESBL-Ec isolated from healthy humans and communal wastewater. METHODS This was an observational study using stored isolates and laboratory data. Genome sequencing was done on 43 E. coli DNA isolates before resistance genes, mutations, and high-risk clones were exam
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Bouaziz, Amira, Lotfi Loucif, Ammar Ayachi, Karima Guehaz, Esma Bendjama, and Jean-Marc Rolain. "Migratory White Stork (Ciconia ciconia): A Potential Vector of the OXA-48-Producing Escherichia coli ST38 Clone in Algeria." Microbial Drug Resistance 24, no. 4 (2018): 461–68. http://dx.doi.org/10.1089/mdr.2017.0174.

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Duffy, Nadezhda, Davina Campbell, Hannah E. Reses, et al. "Epidemiologic Characteristics of ESBL-Producing ST131 E. coli Identified Through the Emerging Infections Program, 2017." Infection Control & Hospital Epidemiology 41, S1 (2020): s214—s215. http://dx.doi.org/10.1017/ice.2020.758.

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Background: Extended-spectrum β-lactamase–producing (ESBL) Escherichia coli infection incidence is increasing in the United States. This increase may be due to the rapid expansion of ST131, which is now the predominant ESBL strain globally, often multidrug resistant, and has been shown to establish longer-term human colonization than other E. coli strains. We assessed potential risk factors that distinguish ST131 from other ESBL E. coli. Methods: From October 1 through December 31, 2017, 5 CDC Emerging Infections Program (EIP) sites pilot tested active, laboratory-based surveillance in selecte
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Martino, Florencia, Alejandro Petroni, María Alejandra Menocal, Alejandra Corso, Roberto Melano, and Diego Faccone. "New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates." PLOS ONE 19, no. 2 (2024): e0294820. http://dx.doi.org/10.1371/journal.pone.0294820.

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Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteria including carbapenem-resistant Enterobacterales. There are ten mcr genes described in different Gram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far the most frequent combination. In Argentina, mcr-1 gene was characterized only on plasmids belonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboring plasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolates carrying the mcr-1 gene were sequenced using next
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Mhaya, Amel, Rahma Trabelsi, Sabine Aillerie, et al. "Detection of Clones B2-ST131-C2 and A-ST617 in Escherichia coli Producing Both CTX-M-15 and CTX-M-27 from Tunisian Community Patients." Antibiotics 11, no. 10 (2022): 1329. http://dx.doi.org/10.3390/antibiotics11101329.

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During a two-month period (2017–2018), 336 urine samples positive for Escherichia coli were collected from Tunisian patients. Of the 336 samples, 266 were collected from community patients and 70 from hospital settings. In all, 15 ESBL producers were identified (8 and 7, respectively) and assigned to 13 pulsotypes, including four ESBL-producing E. coli (ESBL-E) with E1 and E2 profiles (2 isolates each) from community patients. The two strains E1 were identified as B2-ST131 subclade C2 and the two isolates E2, A-ST617. The four strains carrying both CTX-M-15 and CTX-M-27, exhibited the multirep
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Byarugaba, Denis K., Bernard Erima, Godfrey Wokorach, et al. "Resistome and virulome of high-risk pandemic clones of multidrug-resistant extra-intestinal pathogenic Escherichia coli (ExPEC) isolated from tertiary healthcare settings in Uganda." PLOS ONE 18, no. 11 (2023): e0294424. http://dx.doi.org/10.1371/journal.pone.0294424.

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Multi-drug resistant (MDR) globally disseminated extraintestinal pathogenic high-risk Escherichia coli (ExPEC) clones are threatening the gains in bacterial disease management. In this study, we evaluated the genomic structure including the resistome and virulome of the E. coli isolates from extraintestinal infections using whole genome sequencing (WGS). The results highlight that isolates were highly resistant (≥ 90.0%) to commonly used antibiotics (Ampicillin, Trimethoprim-Sulfamethoxazole, Nalidixic acid, and Piperacillin) and were less (&lt;14%) resistant to last resort antibiotics; Imipen
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Kaur, Harpreet, Inderjit Singh, Vinay Modgil, Nisha Singh, Balvinder Mohan, and Neelam Taneja. "Genome sequence of pan drug-resistant enteroaggregative Escherichia coli belonging to ST38 clone from India, an emerging EAEC/UPEC hybrid pathotype." Indian Journal of Medical Microbiology 49 (May 2024): 100606. http://dx.doi.org/10.1016/j.ijmmb.2024.100606.

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Sekizuka, Tsuyoshi, Mari Matsui, Kunikazu Yamane, et al. "Complete Sequencing of the blaNDM-1-Positive IncA/C Plasmid from Escherichia coli ST38 Isolate Suggests a Possible Origin from Plant Pathogens." PLoS ONE 6, no. 9 (2011): e25334. http://dx.doi.org/10.1371/journal.pone.0025334.

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Falgenhauer, Linda, Patrice Nordmann, Can Imirzalioglu, et al. "Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland." International Journal of Antimicrobial Agents 56, no. 6 (2020): 106157. http://dx.doi.org/10.1016/j.ijantimicag.2020.106157.

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Yao, Fanghui, Qian Zhao, Di Wang, and Xuewen Li. "Genetic Characterization, Transmission Pattern and Health Risk Analysis of Intestinal Colonization ESBL-Producing Escherichia coli in Vegetable Farming Population." Microorganisms 12, no. 12 (2024): 2646. https://doi.org/10.3390/microorganisms12122646.

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The surging prevalence rates of ESBL-producing Escherichia coli (ESBL-Ec) pose a serious threat to public health. To date, most research on drug-resistant bacteria and genes has focused on livestock and poultry breeding areas, hospital clinical areas, natural water environments, and wastewater treatment plants. However, few studies have been conducted on drug-resistant bacteria in vegetable cultivation. In this study, a total of vegetable farmers (n = 59) from six villages were surveyed. Fecal samples were collected from vegetable farmers; we also collected environmental samples, including riv
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Hameed, Muhammad Fazal, Yanan Chen, Hazrat Bilal, et al. "The Co-occurrence of mcr-3 and fosA3 in IncP plasmid in ST131 Escherichia coli: A novel case." Journal of Infection in Developing Countries 16, no. 04 (2022): 622–29. http://dx.doi.org/10.3855/jidc.15943.

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Introduction: Plasmid-mediated colistin resistance genes, especially mcr-3 combined with the fosfomycin resistance gene fosA3, are a grave health concern. Our study was designed to determine the epidemiological characteristics of the combination of mcr-3 and fosA3 in Anhui province, China.&#x0D; Methodology: A total of 127 multi-drug-resistant (MDR) E. coli strains were assessed for antibiotic resistance/sensitivity to detect mcr-3 and fosA3 using polymerase chain reaction (PCR) and sequencing. The genes of interest were conjugated using EC600, and replicon and sequence types (STs) were identi
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Pankok, Frederik, Frieder Fuchs, Ulrike Loderstädt, et al. "Molecular Epidemiology of Escherichia coli with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016." Microorganisms 10, no. 12 (2022): 2448. http://dx.doi.org/10.3390/microorganisms10122448.

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Colonization and infection with bacteria with acquired antibiotic resistance are among the risks for soldiers on international deployments. Enterobacterales with resistance against third-generation cephalosporines are amongst the most frequently imported microorganisms. To contribute to the scarcely available epidemiological knowledge on deployment-associated resistance migration, we assessed the molecular epidemiology of third-generation cephalosporine-resistant Escherichia coli isolated between 2007 and 2016 from German soldiers after deployments, with a particular focus on the African Sahel
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Guiral, Elisabet, Milene Gonçalves Quiles, Laura Muñoz та ін. "Emergence of Resistance to Quinolones and β-Lactam Antibiotics in Enteroaggregative and Enterotoxigenic Escherichia coli Causing Traveler’s Diarrhea". Antimicrobial Agents and Chemotherapy 63, № 2 (2018): e01745-18. http://dx.doi.org/10.1128/aac.01745-18.

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ABSTRACT The objective of this study was to assess the antimicrobial resistance of enteroaggregative Escherichia coli (EAEC) and enterotoxigenic E. coli (ETEC) strains causing traveler’s diarrhea (TD) and to investigate the molecular characterization of antimicrobial resistance genes to third-generation cephalosporins, cephamycins, and quinolones. Overall, 39 EAEC and 43 ETEC clinical isolates were studied. The susceptibilities of EAEC and ETEC against ampicillin, amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin, az
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Markovska, Rumyana, Petya Stankova, Temenuga Stoeva, et al. "Dissemination of High-Risk Clones Enterobacterales among Bulgarian Fecal Carriage Isolates." Microorganisms 10, no. 11 (2022): 2144. http://dx.doi.org/10.3390/microorganisms10112144.

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The gastrointestinal tract is an important reservoir of high-risk Enterobacteria clones and a driver of antimicrobial resistance in hospitals. In this study, patients from six hospitals in four major Bulgarian towns were included in this study. Overall, 205 cefotaxime-resistant isolates (35.3%) of Enterobacterales order were detected in fecal samples among 580 patients during the period of 2017–2019. ESBL/carbapenemase/plasmidic AmpC producer rates were 28.8%, 2.4%, and 1.2%, respectively. A wide variety of ESBLs: CTX-M-15 (41%), CTX-M-3 (24%), CTX-M-27 (11%), and CTX-M-14 (4%) was found. The
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Rafaque, Z., J. I. Dasti, and S. C. Andrews. "Draft genome sequence of a uropathogenic Escherichia coli isolate (ST38 O1:H15) from Pakistan, an emerging multidrug-resistant sequence type with a high virulence profile." New Microbes and New Infections 27 (January 2019): 1–2. http://dx.doi.org/10.1016/j.nmni.2018.10.004.

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MEZIANI, Meriem, and Kaddour BENLABED. "Carbapenem resistance due to BlaOXA-48 Clone ST38 among ESBL-producing Escherichia coli isolates from patients hospitalised at the university hospital of Constantine in Algeria." International Journal of Advanced Research 4, no. 7 (2016): 36–42. http://dx.doi.org/10.21474/ijar01/903.

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