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Dissertations / Theses on the topic 'Colorimetric method'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Olsson, Hanna. "Smartphone-based Colorimetric Diagnosis : DEVELOPMENT OF A METHOD FOR AUTOMATIC COMPENSATION OF IMPACT OF LIGHT SETTING." Thesis, KTH, Skolan för teknik och hälsa (STH), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173907.

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During the last years many mobile health applications have emerged on the market. Most of these collect and compiles physical data that can be followed over time. Now the next generation of health care applications are on their way. With an increasing capacity and high quality sensors, smartphones have the potential to be used as diagnostic tools. Calmark Sweden AB is a company that has developed a smartphone based diagnostic platform for analysis of colorimetric assays integrated on a disposable plastic chip. Their first product, the hilda Neo system is a Point of care test (POCT) for semi qu

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Ghosh, Debabrata. "Pharmacognosy of different clones of Camellia sinensis (L.) O. Kuntze, cultivated in Darjeeling hills with special emphasis on the establishment of a new and rapid colorimetric method for quantitative estimation of caffeine." Thesis, University of North Bengal, 2000. http://hdl.handle.net/123456789/1068.

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Lui, Manjuan. "Studies in combretaceae : development of a colorimetric microdilution antifungal screen and a computational method for characterisation of phytochemicals." Thesis, University of Strathclyde, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441880.

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4

Dai, Jianming. "Microwave-assisted extraction (MAE) of neem and the development of a colorimetric method for the determination of azadirachtin related limonoids (AZRL)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape3/PQDD_0035/MQ64339.pdf.

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5

Yang, Lixia. "Local investigations of gas-liquid mass transfer around Taylor bubbles flowing in straight and meandering millimetric channels using a colorimetric method." Thesis, Toulouse, INSA, 2017. http://www.theses.fr/2017ISAT0027/document.

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Les réacteurs-échangeurs à plaques (HEX) sont une technologie clé en intensification des procédés. Cependant, la plupart des recherches existantes portant sur ce type d'équipement ont été réalisées dans le cas d’écoulements monophasiques. Pour les réactions gaz-liquide, peu d'études ont été conduites. Cette thèse a pour objectif d’étudier localement le transfert de matière gaz-liquide autour de bulles de Taylor en écoulement dans des canaux millimétriques droits et ondulés par une méthode dite colorimétrique. Ceci constitue une étape préliminaire indispensable avant la mise en œuvre de système

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Slabbert, Chrizaan. "Evaluation and validation of methods to determine parasitemia in malaria cell cultures / Chrizaan Slabbert." Thesis, North-West University, 2008. http://hdl.handle.net/10394/2332.

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Dinh, Quang Hung. "Development of a Rapid and Easy Measurement Protocol for Perfluorinated Carboxylic Acids (PFCAs) by a Continuous Flow Analysis." 京都大学 (Kyoto University), 2016. http://hdl.handle.net/2433/204596.

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8

Kamoi, T. "Developing an optimal method for producing a tearless onion." Diss., Lincoln University, 2008. http://hdl.handle.net/10182/1008.

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People experience the irritating tearing and burning sensation of lachrymatory factor (LF, propanthial S-oxide) when cutting or chopping onion bulbs. LF is produced by lachrymatory factor synthase (LFS) specifically from 1-propenyl sulfenic acid, a breakdown product of trans-1-propenyl-L-cysteine sulfoxide (1-PRENCSO) by alliinase. This thesis describes strategies to produce a tearless onion by using RNA interference (RNAi) silencing. To determine whether a gene silencing cassette can silence lfs gene transcripts from onion (Allium cepa L.), a crop recalcitrant to genetic transformation, a gen

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Ferraz, Neto José. "Desenvolvimento de metodologia para determinação de matéria orgânica do solo por análise de imagens." Universidade do Estado de Santa Catarina, 2015. http://tede.udesc.br/handle/handle/2313.

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Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2017-12-05T12:41:21Z No. of bitstreams: 1 PGCS15MA124.pdf: 1312847 bytes, checksum: 750d88452b24e573464fff88790dbef6 (MD5)<br>Made available in DSpace on 2017-12-05T12:41:21Z (GMT). No. of bitstreams: 1 PGCS15MA124.pdf: 1312847 bytes, checksum: 750d88452b24e573464fff88790dbef6 (MD5) Previous issue date: 2015-05-15<br>The soil organic matter (SOM) is formed by a set of chemical compounds which have different decomposition rates and products of these processes result as the main source of sulfur, phosphorus and nitrogen into the soil. Beca

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Barrios, Marco Rogério. "Contribuição à automação do processo de flotação do fosfato de cajati." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/3/3134/tde-18082016-081606/.

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Flotação é uma operação unitária à base de química de superfície que envolve a captura de partículas hidrofóbicas por bolhas de ar na suspensão aquosa, na qual são transportadas para a superfície, com posterior remoção desta camada de espuma. Por outro lado, porque as partículas hidrofílicas não aderem a bolhas de ar, elas tendem a afundar e ir para o fluxo inferior do tanque de flotação. A separação de apatita-ganga através de flotação de espuma é o controle eficaz dos fenômenos de molhabilidade, que, por sua vez, determina a seletividade do processo. A regulação da interação de minerais (apa

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11

Silva, Mariana Rosa da. "Padronização de método colorimétrico para avaliação de atividade biológica de substâncias sobre formas taquizoítas de Toxoplasma gondii, com a avaliação de triterpenos ácidos sobre o parasito." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29052009-154530/.

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Toxoplasma gondii é um protozoário pertencente ao filo Apicomplexa, de distribuição mundial, e que infecta diversas espécies hospedeiros, como mamíferos e aves, possuindo como hospedeiros definitivos o gato e outros felídeos, enquanto o homem e outros animais são os seus hospedeiros intermediários. O tratamento é feito, na maioria das vezes, com uma combinação de sulfadiazina e pirimetamina, agindo na via metabólica do ácido fólico, o qual é necessário para a biossíntese de purinas, pirimidinas e certos aminoácidos. Propusemos estabelecer um protocolo de avaliação sobre esse protozoário, assim

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Martins, Luciana Foltram. "Desenvolvimento de equipamento de baixo custo instrumentalizar o metodo ASTM D 1209-00." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250214.

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Orientadores: Adriana Vitorino Rossi, Matthieu Tubino<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-11T04:54:42Z (GMT). No. of bitstreams: 1 Martins_LucianaFoltram_M.pdf: 1935744 bytes, checksum: f05f16f5c0380e513d0ae7b7af183efe (MD5) Previous issue date: 2008<br>Resumo: O método ASTM D 1209-00 descreve o procedimento para a medida visual da coloração de líquidos levemente coloridos, que envolve a comparação visual entre a coloração de amostras com a coloração de padrões Pt-Co com concentração variando de 1 a 500 na

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North, Amy D. "Investigation of observer variability using a new method for determining color matching functions /." Online version of thesis, 1991. http://hdl.handle.net/1850/11099.

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Petersen, Kelvin H. "An investigation of new methods to improve the accuracy of some modern color spectrophotometers /." Online version of thesis, 1989. http://hdl.handle.net/1850/11548.

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Miwa, Adriana Cristina Poli. "Comparação e avaliação dos métodos colorimétricos utilizados para determinação de proteínas em lagoas de estabilização." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-17042006-142148/.

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As lagoas de estabilização representam um dos métodos mais simples utilizados para tratamento de esgoto sanitário. Grande parte da matéria orgânica presente no esgoto sanitário é constituída por proteínas, a qual torna-se fator limitante aos processos biológicos de tratamento de águas residuárias em que se encontram presentes. O objetivo deste trabalho foi avaliar e comparar métodos colorimétricos para determinação de proteínas, definindo qual é o mais apropriado para esse tipo de ambiente. Os métodos colorimétricos utilizados foram o de Lowry, de Bradford, do Biureto e do Ácido Bicinconínico.

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Peters, Kelley L. "Development of Presumptive and Confirmatory Analytical Methods for the Simultaneous Detection of Multiple Improvised Explosives." FIU Digital Commons, 2014. http://digitalcommons.fiu.edu/etd/1659.

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In recent years, there has been a dramatic increase in the use of improvised explosive devices (IEDs) due to ease of synthesis and improved controls placed on commercial/military explosives. Commonly used materials for IED preparations include fertilizers and industrial chemicals containing oxidizers such as ClO3-, ClO4-, and NO3-, as well as other less stable compounds, such as peroxides. Due to these materials having a wide range of volatility, polarity, and composition, detection can be challenging, increasing the amount of time before any analytical information on the identity of the explo

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17

Mori, Eva Kaiser. "Caracterização de pinturas do artista Alfredo Volpi por meio de métodos não destrutivos: espectrofotômetro, EDXRF, MEV e imageamento multiespectral." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/44/44144/tde-17112015-151200/.

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Alfredo Volpi (1896 - 1988) é um dos mais importantes pintores brasileiros contemporâneos. Sua extensa obra foi marcada, principalmente, por composições cromáticas e abstrações geométricas. Foi um artista que dava importância muito grande à técnica e aos materiais, tendo desenvolvido uma maneira própria e sistemática de trabalhar: confeccionava suas próprias telas, tintas e até pigmentos, com terras naturais. Analisou-se ao todo 11 pinturas a têmpera em diversos suportes (tela, aglomerado de madeira e mural), 10 delas realizadas na década de 1950, período em que a obra do artista passou

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Mizumoto, Camilo [UNESP]. "Investigação da reação álcali-agregado (RAA) em testemunhos de concreto e agregados constituintes." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/91463.

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Made available in DSpace on 2014-06-11T19:25:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-29Bitstream added on 2014-06-13T20:32:50Z : No. of bitstreams: 1 mizumoto_c_me_ilha.pdf: 6141199 bytes, checksum: d708c1fab69dfe7948269a1d6ffae9a2 (MD5)<br>Fundação de Ensino Pesquisa e Extensão de Ilha Solteira (FEPISA)<br>A reação álcali-agregado (RAA) é um fenômeno expansivo que ocorre em diversas estruturas de concreto que vivenciam freqüentes condições de umidade. O princípio desta reação fundamenta-se em interações químicas entre a sílica constituinte do agregado e os álcalis prese

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Mizumoto, Camilo. "Investigação da reação álcali-agregado (RAA) em testemunhos de concreto e agregados constituintes /." Ilha Solteira : [s.n.], 2009. http://hdl.handle.net/11449/91463.

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Orientador: Antonio Anderson da Silva Segantini<br>Banca: Selmo Chapira Kuperman<br>Banca: Marcelo Ornaghi Orlandi<br>Resumo: A reação álcali-agregado (RAA) é um fenômeno expansivo que ocorre em diversas estruturas de concreto que vivenciam freqüentes condições de umidade. O princípio desta reação fundamenta-se em interações químicas entre a sílica constituinte do agregado e os álcalis presentes no concreto, em presença de umidade. O produto formado é um "gel" expansivo que ocasiona processos de fissuração e deslocamentos diferenciais em estruturas de concreto. Atualmente, vários centros de pe

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Guglielmino, Maud. "Développement d'une nouvelle méthode analytique du formaldéhyde dans l'air basée sur un dispositif microfluidique." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAF048.

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Le formaldéhyde (HCHO) est un polluant majeur de l’air intérieur. L’objectif de cette thèse est de réaliser les avancées scientifiques et technologiques nécessaires à l’obtention d’une méthode analytique basée sur un dispositif microfluidique de mesure du formaldéhyde dans l’air associant précision, sélectivité, rapidité d’analyse avec pour objectif majeur une autonomie suffisante sur de longues durées, typiquement un mois. Le principe de la méthode reposait initialement sur trois étapes clés, à savoir le piégeage du formaldéhyde gazeux en solution, la réaction du formaldéhyde avec un agent dé

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Van, Houwelingen ArJan. "Liquid-solid contacting in trickle-bed reactors." Thesis, University of Pretoria, 2009. http://hdl.handle.net/2263/30008.

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Several types of reactors are encountered in industry where reagents in a gas and a liquid phase need to be catalysed by a solid catalyst. Common reactors that are used to this end, are trickle-bed reactors, where gas and liquid flow cocurrently down a packed bed of catalyst. Apart from the catalytic process itself, several mass transfer steps can influence the rate and/or selectivity of a solid catalysed gas-liquid reaction. In trickle-bed reactors, flow morphology can have a major effect on these mass transfer steps. This study investigates the interaction between liquid flow morphology and

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22

Dias, Liliana Sofia Ribeiro. "Optimization of a colorimetric method for determination of fluoride in saliva." Master's thesis, 2012. https://repositorio-aberto.up.pt/handle/10216/87917.

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Dias, Liliana Sofia Ribeiro. "Optimization of a colorimetric method for determination of fluoride in saliva." Dissertação, 2012. https://repositorio-aberto.up.pt/handle/10216/87917.

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Eriksson, Louise. "Validation of a colorimetric method for determination of fructosamine in plasma usingMindray BS-380." Thesis, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-349084.

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HbA1c and glucose are the most widely used indicators for glucose control, but they havesome disadvantages. Improving the diagnosis of diabetes is always ongoing, other markersare needed as a complement when standard measurements are not sufficient. One alternativeis analysis of fructosamine, which is commercially available and inexpensive.The main aim with this study was to validate a colorimetric method for analyzingfructosamine including investigation of precision, linearity and stability. Fructosamine valueswas compared with HbA1c values with and without genetic variations in the hemoglobi

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Hwang, Kuan-Luen, and 黃冠綸. "Interference Study of the Colorimetric Method for the Determination of Dissolved Oxygen in Seawater." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/93641270020129813057.

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碩士<br>國立臺灣大學<br>海洋研究所<br>94<br>Four potential interference factors involved in colorimetric determination of dissolved oxygen were studied. Those factors include the concentrations of oxygen and iodide in the sample and reagent, the wavelength and temperature at which the detection is made. Evaluations were made both theoretically and experimentally on the linearity of the calibration curve. The results show that concentrations of dissolved oxygen and iodide cause variation of the ratio between molecular iodine and tri-iodide, thus leading to a bending phenomenon of the calibration curve. Dete

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Wang, Tzu-Pao, and 王子豹. "The Influence on Different Voltages of Accelerated Chloride Migration Test by Using Colorimetric Method." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/41931470175376726852.

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碩士<br>國立臺灣海洋大學<br>材料工程研究所<br>94<br>Abstract The accelerated chloride migration test was used to investigate the different voltages (24V, 30V, and 48V), different w/b ratios (0.35, 0.45, 0.55, and 0.65) ,different ages (28days, 91days) and the ordinary Portland cement concrete and concrete containing different type of mineral admixtures (fly ash, slag and ash with slag replacement for 20%, 40% and 30% weight of the total cementitious content) effect of the total charge passeds in concrete. The colorimetric method test was used to measure the chloride ion penetration depth in specimen of concr

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"Novel Colorimetric Sensors with Extended Lifetime for Personal Exposure Monitoring." Doctoral diss., 2019. http://hdl.handle.net/2286/R.I.55643.

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abstract: Air pollution has been linked to various health problems but how different air pollutants and exposure levels contribute to those diseases remain largely unknown. Researchers have mainly relied on data from government air monitoring stations to study the health effects of air pollution exposure. The limited information provided by sparse stations has low spatial and temporal resolution, which is not able to represent the actual exposure of individuals. A tool that can accurately monitor personal exposure provides valuable data for epidemiologists to understand the relationship betwee

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Lin, Yu-Lung, and 林裕榮. "Application of gold nanoparticles colorimetric method for the detection of mercury ion in aqueous solution." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/25763130916226331235.

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碩士<br>高雄醫學大學<br>醫藥暨應用化學研究所<br>101<br>Mercury (II) ion (Hg2+) not only has high toxicity but also causes serious pollution problems. Meanwhile, it also harms people’s health. Therefore, to develop an easy and quick way to detect Hg2+ has become important. This research aims to The main principle of this study is to use the gold nanoparticles’ surface plasmon resonance (SPR) effect. During the detection, the aggregation of AuNPs after the addition of aggregation agents is affected by the presence of Hg2+ and the degree of aggregation shows an inverse proportional to the concentration of Hg2+. Th

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Zhong, Rui-Gong，, and 鍾瑞恭. "Using the Rapid Chloride Migration to Determine the Difference between Colorimetric Method and Chloride Profile." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/3qyhvu.

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碩士<br>國立臺灣海洋大學<br>材料工程研究所<br>107<br>According to the mechanism of ion transport, it can be divided into natural diffusion test and electrical migration test generally. In order to improve efficiency, many researchers adopt the electrical migration test, such as Rapid Chloride Penetration Test (RCPT) and Rapid Chloride Migration Test (RCM). Because Rapid Chloride Penetration Test (RCPT) is controversial, we took Rapid Chloride Migration Test (RCM) to do the research. Rapid Chloride Migration Test (RCM) used Colorimetric method to calculation non-steady state migration coefficient. This test may

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Su, Sheng-Yuan, and 蘇聖淵. "Development of rapid colorimetric assay and real-time duplex LAMP method to detect viral nucleic acid." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/48596477047745340466.

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碩士<br>國立臺灣大學<br>醫學檢驗暨生物技術學研究所<br>100<br>Loop-mediated isothermal amplification (LAMP) was established by Notomi et al. (2000). It uses 4-6 specific primers to recognize the target gene sequence and rapidly amplify under isothermal condition. Using this technique, we established a rapid colorimetric assay and a real-time duplex LAMP (dLAMP) method for viral nucleic acid detection. We anticipated that these two assays could be applied in blood screening, clinical diagnosis, and epidemiological investigation in the future. In the rapid colorimetric assay, we first optimized and validated the HCMV

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Chiu, Yu-An, and 邱鈺安. "Research of substrate specificity and a quick colorimetric activity assay method of UDP-glycosyltransferase from Bacillus cereus." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/22122637940286054559.

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碩士<br>國立交通大學<br>應用化學系分子科學碩博士班<br>99<br>Glycosyltransferases have many important biological functions in all species and in the use of disease treatment. Thus we need a real time and high-throughput method to analyze glycosyltransferase activity. In this research, we focus on the UDP-glycosyltransferase from Bacillus cereus (BcGT-1) catalytic reaction. BcGT-1 was successfully over-expressed in E. coli BL21 (DE3) and purified by DEAE column. In this research, we use a quick colorimetric activity assay for glycosyltransferase BcGT-1. The assay principle is based on the glycosylation activity of B

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Lee, Cheng-Ho, and 李承和. "High Precision Colorimetric Method for the Determination of Dissolved Oxygen in Seawater and Studies of Possible Interfering Factors." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/03230894811690077672.

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碩士<br>國立臺灣大學<br>海洋研究所<br>101<br>This study investigates effects of temperature, iodide concentration, oxidants and suspended solid on the colorimetric determination of oxygen in natural waters. Since the measurement involves the mixture of two iodine species (i.e. I2 and I3-), the equilibrium between them becomes a crucial factor to the precision of the method. A high concentration of iodide in the pickling reagent was found essential to ensure a stable [I_3^- ]/[I_2 ] ratio. Besides, it was also found that elevated temperature can cause a lower signal of molecular iodine, but increase the abs

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Kaur, Daljit. "Development of a cheap and rapid method to determine calcium in milk fractions in an industrial environment." 2007. http://hdl.handle.net/10292/742.

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Milk contains high concentrations of calcium. It occurs in two forms, a free ionic form, and calcium associated with milk proteins (caseins). The latter association is called colloidal calcium phosphate. New Zealand Dairy Foods of Takanini is marketing a range of commercial milks in supermarkets. The company uses ultra filtration to concentrate milk proteins and calcium in different milk products. During ultra filtration, the fraction that is retained by the membrane is rich in calcium bound to proteins and the portion that passes through the membrane is richer in the free ionic form. The comp

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Wu, Cheng-Chieh, and 吳承杰. "A WEIGHTED MASKING MODEL BASED ON THE MODIFIED METHOD OF UNDER COLOR REMOVAL FOR ACCURATE COLORIMETRIC CHARACTERIZATION OF LCDS." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/12039913472122815774.

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碩士<br>大同大學<br>通訊工程研究所<br>96<br>Flat-panel displays have become increasingly popular because of its low power consumption and portability. In a color management system (CMS), accurate colorimetric characterization of a display device plays an important role in achieving device-independent color reproduction. In the case of a CRT display, colorimetric characterization based on a gain-offset-gamma (GOG) model is accurate enough for this purpose. However, that is not effective to characterize the colorimetry of liquid crystal displays (LCDs) since there are many difficulties, such as channel inter

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Li, Wong-Ta, and 李旺達. "The Influence of Chloride Concentration in Color Change Region with Colorimetric Method on the Non-steady-state Chloride Diffusibility of Concrete." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/58904069461795974065.

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碩士<br>國立臺灣海洋大學<br>材料工程研究所<br>94<br>The purpose of this thesis is using Accelerated Chloride Migration Test ( ACMT ) and the colorimetric method to determinate the non-steady -state chloride migration coefficient. To make sure the chloride ion must not pass through the concrete specimen, and to discuss the chloride migration coefficient under the non-steady-state situation, this thesis using ACMT with concrete specimen of 5 cm thickness and 24 hours test duration. After ACMT, the analysis of experiment is divided into two parts. One is to use colorimetric method to determinate the chloride pene

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Lee, Yi-Hsin, and 李奕欣. "Implementation of a fast indophenol blue colorimetric method for the determination of dissolved total ammonia using manganese (II) as a catalyst." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/ra6yqm.

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碩士<br>國立臺灣大學<br>海洋研究所<br>106<br>The indophenol blue method for the determination of dissolved ammonia has been reassessed in several analytical respects including pH control, reaction kinetics, choice of reagents and reagent stability. It was found that an optimal reaction rate (t99%<3 min at room temperature) can be achieved using phenol as the coupling agent, hypochlorite as the oxidant and manganese (II) as the catalyst. The key conditions are that the formation of the intermediate product-chloramine needs to form at pH 7-8, which further reacts with phenol at pH 8-8.5 and the final formati

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Chen, Li-fei, and 陳麗妃. "Study of Colorimetry Using Vat Dyes with Padding Method on Cotton." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/28136330435321989898.

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碩士<br>逢甲大學<br>紡織工程所<br>96<br>Cotton and cellulose fibers are very important textile. They are used in whole world and their consumption is increasing. Vat dye is one of the most popular dye classes used for coloration of cotton in today’s dyeing industry because vat dyes yield coloured fiber of excellent all-round fastness, particularly to light, washing and chlorine bleaching. Vat dyes play an important role in blue jeans. Although only 10 g of indigo is necessary for dyeing one pair of trousers, the vast annual sales of 109 blue jeans in the whole world. By the trend of time, the quality of

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Zunnoon, Khan Sara. "Towards Development of an Immunoassay Utilizing Circularly Permutated Proteins to Detect Environmental Contaminants." Thesis, 2013. http://hdl.handle.net/10214/7434.

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A fusion protein composed of antibody fragments and β-lactamase was earlier created by Kojima et al. (2011), with antigen specificities against a bone disease marker and a pesticide. The enzyme was circularly permutated and fused to the variable heavy and light chain antibody fragments, thereby ensuring inactivity until binding of the target antigen triggered enzyme activation. Upon activation, the β-lactamase produced a colorimetric signal, which indicated antigen presence. In this work, a similar strategy was used to create two novel fusion proteins composed of circularly permuted β-lactama

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39

Cheng, Chia-Lun, and 鄭家侖. "comparison of the two modified colorimetric methods on the feasibility of measuring oxygen in acidic mine water." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/44248655324408219220.

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碩士<br>國立臺灣大學<br>海洋研究所<br>95<br>Oxygen in acidic mine water is difficult to measure by colorimetry because such water contains high amount of dissolved iron which interferes. Both ferric and ferrous ions produce excess molecular iodine and triiodide thus leading to overestimated results. To solve this problem, a modified colorimetric method is proposed in which the sample is added with permanganate prior to the pickling procedure and all ferrous ions are converted to ferric ions. The sample was further treated with K2C2O4 and KF to remove the interference of permanganate and ferric ions. After

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