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Journal articles on the topic 'Colorimetric'
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Lixiu, He, Hu Yuefei, and Lv Zhiqiao. "Research Progress in Detection of Explosives by Chemical Colorimetric Method." Expert Review of Chinese Chemical 2, no. 1 (2024): 1–4. http://dx.doi.org/10.62022/ercc.issn3006-0095.2024.01.001.
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This article reviews the current application status and research progress of colorimetric detection methods based on chemical colorimetry in the detection of explosives.It mainly introduced colorimetric sensors, colorimetric sensor arrays, and chemical colorimetric sensors based on novel material substrates. The application prospect of chemical colorimetric method in the field of explosives detection was prospected.
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Xu, Ning, Shuang Jin, and Li Wang. "Metal nanoparticles-based nanoplatforms for colorimetric sensing: A review." Reviews in Analytical Chemistry 40, no. 1 (2020): 1–11. http://dx.doi.org/10.1515/revac-2021-0122.
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Abstract With the progress of analysis technology and nanotechnology, colorimetric detection has become one of the research hotspots in the field of analytical chemistry. Compared with traditional detection methods, the colorimetric method has many advantages, such as high sensitivity, good selectivity, convenience and fast, as well as low cost. In recent years, metal nanoparticles have been introduced into colorimetry, making the research and application of colorimetry develop rapidly. In this work, we summarize the usual colorimetric detection methods based on metal nanoparticles-based nanozymes and their applications in the last five years. We hope that this work will help readers understand the mechanism and practical application value of nanozyme-based colorimetric biosensors. Meanwhile, this work may give some hints and references for future colorimetric detection research to promote the application and development of nanozyme-based colorimetry in biomedical and environmental analysis.
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Yefymenko, Serhii, Ihor Hryhorenko, Iurii Khoroshailo, and Svitlana Hryhorenko. "Applying covariance analysis to determine the factor influence on the control parameter in colorimetric study." Ukrainian Metrological Journal, no. 3 (September 30, 2022): 49–55. http://dx.doi.org/10.24027/2306-7039.3.2022.269783.
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The paper considers solutions to a scientific and practical problem of applying covariance analysis to determine the factor influence on the functional transformation of the control parameter in colorimetric study. The study implies determining the factor influence on the additive and multiplicative components of the measurement error of the colorimetric control parameter to assess the validity of conclusions about the factor influence on the transformation of the control parameter. The limitations on the number of basic levels (control parameters) and the factors influencing the result of colorimetric control are determined. During the study, the equations to assess the validity of statistical conclusions about the informational significance of the colorimetric control indicators for a simplified model of cross-classification were obtained.
 The need for the study is due to the fact that in colorimetric control of cereal grains, the measurement uncertainty of the results of measuring the values of the controlled indicators at given levels of the control parameter is quite high.
 In modern industry, colorimetry has a number of advantages over other methods, such as weight analysis. Colorimetric determinations are performed much faster. In the case of weight analysis, the chemical reaction is at the beginning of the determination followed by a series of long operations, while in the case of colorimetry, the colors are compared immediately after the chemical reaction. Therefore, the colorimetric method belongs to the methods of express control.
 The main task of colorimetric express control (in our case) is to determine the quality of cereal grains. The task is important and relevant because grain, namely flour, is the main ingredient of many foods, e.g. bread, pasta, pastries, and cookies. Quality products can be obtained only from quality raw materials. Thus, at present time, it is relevant to determine the quality of cereal grains, and flour as the derived product, in Ukraine.
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Pierre, Lissage, Julio Elías Bruna Bugueño, Patricio Alejandro Leyton Bongiorno, Alejandra Torres Mediano, and Francisco Javier Rodríguez-Mercado. "Colorimetric Indicator Based on Gold Nanoparticles and Sodium Alginate for Monitoring Fish Spoilage." Polymers 16, no. 6 (2024): 829. http://dx.doi.org/10.3390/polym16060829.
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In this work, a colorimetric indicator based on gold nanoparticles (AuNP) and a biodegradable and eco-friendly polymer (sodium alginate, Alg.), was developed for the real-time detection of fish spoilage products. The AuNPs and the colorimetric indicator were characterized using UV-VIS, FTIR spectroscopies, TGA, DSC, XRD, TEM, and colorimetry. The UV-VIS spectrum and TEM showed the successful synthesis, the spherical shape, and the size of AuNPs. The results indicated color changes of the indicator in packaged fish on day 9 of storage at a refrigerated temperature (5 °C. These results showed the successful application of the colorimetric indicator in the detection of TVB-N in packaged fish.
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Li, Xianming, Lu Gao, Feng Li, Xiandeng Hou, and Peng Wu. "Universal and label-free photosensitization colorimetric assays enabled by target-induced termini transformation of dsDNA resistant to Exo III digestion." Chemical Communications 55, no. 50 (2019): 7211–14. http://dx.doi.org/10.1039/c9cc03551f.
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Matula, J. "Determination of dissolved reactive and dissolved total phosphorus in water extract of soils." Plant, Soil and Environment 57, No. 1 (2011): 1–6. http://dx.doi.org/10.17221/185/2010-pse.
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The simplified acid peroxydisulphate digestion of soil water extract was evaluated for determination of total dissolved phosphorus by molybdenum-blue colorimetry in comparison with direct P-detection in water extract by the ICP-AES technique. The research was conducted on 79 agricultural soils with different agrochemical characteristics. The results of the colorimetric P detection in water extract without digestion and ICP phosphorus detection were different. The median of values determined by ICP-AES was 1.7 times higher than that of colorimetry, but the correlations between the two measurements were quite close (r = 0.993). Differences between the colorimetric phosphorus and ICP-AES phosphorus were irregular, increasing as the phosphorus level in soils decreased. The simplified procedure of acid peroxydisulphate digestion is useful for routine determination of total water-extracted phosphorus in soils when the soil testing laboratory is not equipped with the ICP-AES technique. The two-tailed paired t-test did not prove any difference in the values between the direct ICP-AES P-detection in water extract of soils and colorimetric P-detection in the acid peroxydisulphate digest.  
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Majdinasab, Marjan, Sondes Ben Aissa, and Jean Louis Marty. "Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring." Toxins 13, no. 1 (2020): 13. http://dx.doi.org/10.3390/toxins13010013.
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Mycotoxins contamination is a global public health concern. Therefore, highly sensitive and selective techniques are needed for their on-site monitoring. Several approaches are conceivable for mycotoxins analysis, among which colorimetric methods are the most attractive for commercialization purposes thanks to their visual read-out, easy operation, cost-effectiveness, and rapid response. This review covers the latest achievements in the last five years for the development of colorimetric methods specific to mycotoxins analysis, with a particular emphasis on their potential for large-scale applications in food industries. Gathering all types of (bio)receptors, main colorimetric methods are critically discussed, including enzyme-linked assays, lateral flow-assays, microfluidic devices, and homogenous in-solution strategies. This special focus on colorimetry as a versatile transduction method for mycotoxins analysis is comprehensively reviewed for the first time.
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AMELIN, VASILY G., SHOGAH ZEN ALABDEN CHALAWI, and DMITRY S. BOLSHAKOV. "IDENTIFICATION AND AUTHENTICATION OF COW’S MILK POWDER USING A SMARTPHONE AND CHEMOMETRIC ANALYSIS." Lomonosov chemistry journal 64, no. 1, 2023 (2023): 49–59. http://dx.doi.org/10.55959/msu0579-9384-2-2023-64-1-49-59.
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The possibility of identi cation and authentication of cow’s milk powder by the method of chemical colorimetry of the samples’ own uorescence using a smartphone camera and chemometric analysis is shown. A fundamental test device for direct colorimetric analysis of powdered milk is proposed, excluding the stage of sample preparation. Fluorescence excitation was carried out with a portable source of monochromatic UV radiation (λ = 365 nm). Colorimetric parameters of powdered milk uorescence in RGB space were recorded using a smartphone camera. We used chemometric processing of the calculated analytical signal, which made it possible to reduce the analysis time and visualize the study data. The evaluation of the data array of uorescence colorimetric parameters (RGB) was carried out by principal component analysis (PCA) and hierarchical clustering analysis (HCA) using the XLSTAT software.
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Jiang, Lei, Shi Sheng Zhou, and Ying Cui. "Model of Ink Quantity Control Based on Colorimetry System." Applied Mechanics and Materials 262 (December 2012): 258–62. http://dx.doi.org/10.4028/www.scientific.net/amm.262.258.
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Abstract. In the offset printing process，the ink quantity control in the ink zone, color and ink quantity transformation algorithm and the establishment of database，are all based on principle of density measurement. With the establishment and wide spread use of the standard ISO12647-2 for offset, colorimetric measurement is becoming a tendency. By experimental research，this article analyses the relationship between ink thickness and colorimetric value(L*a*b*) by regression method, determines the best ink quantity parameter, establishes the model of ink quantity controlling based on colorimetry system.
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Yoon, Jiyoung, Cheolhwan Kim, and Shihyun Ahn. "Colorimetric Matching for Medical Cameras." International Journal of Computer and Electrical Engineering 8, no. 3 (2016): 227–33. http://dx.doi.org/10.17706/ijcee.2016.8.3.227-233.
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Zhbanova, Vera L. "Features Of Digital Colourimetry Application in Modern Scientific Research." Issue 03-2021, no. 03-2021 (June 2021): 146–58. http://dx.doi.org/10.33383/2021-028.
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The paper is devoted to digital colorimetry, has a character of review, and deals with the main issues of colorimetric calculations and transformations during digital colour registration. The presented materials are intended to help researchers already at the stage of registration to exclude colour losses and, accordingly, errors of measurements, taking into account the following features: – The connection of colorimetric measurements with digital circuit design is shown, which results in digital colorimetry as a tool for acquiring and processing accurate colour information about the object of study; – Important issues such as: selection criteria for parameters of photodetector arrays, colour separation systems, working colour spaces, formats of colour images, and post-processing methods for colour information of images; – Colour separation systems of photodetector arrays, their advantages and disadvantages, influence on the registration result and prospects of new developments in this field are described; – Working colour spaces of digital recording systems, and also colour systems are considered allowing maximum saving colour parameters of digital impression; – To understand human colour perception, the problem of hardware-independent reproduction of colour and images after image registration is considered; – It is proposed to use such colorimetric systems in the analysis of colour parameters in scientific studies, which give understanding of human image perception in the analysis of image quality. The paper gives a broad overview of the digital colorimetry main aspects and researches on this topic for specialists who use digital colour recorders as a tool in their experimental research. This material can also be useful for specialists with deep knowledge in colorimetry who use digital colour recorders as part of their main tasks in the definite field of science and technology.
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Garmay, Andrei V., Oksana V. Monogarova, Kirill V. Oskolok, and Pavel М. Radzikovitskiy. "DETERMINATION OF NITRATE IN SOILS BY DIGITAL COLORIMETRY." Lomonosov chemistry journal 66, no. 1, 2025 (2025): 52–59. https://doi.org/10.55959/msu0579-9384-2-2025-66-1-52-59.
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A method for determining nitrate in soil by digital colorimetry is proposed. The developed approach is based on appropriate photometric techniques of nitrate determination after potassium chloride extraction from soil samples. The original method of determining nitrate in the form of an azo dye formed by the azo coupling reaction between 1-naphtylamine and sulfanilamide has been modifi ed to achieve a higher sensitivity and accuracy of colorimetric determination. This approach ensures metrological characteristics of the digital colorimetric technique, close to spectrophotometric. The adequacy of the proposed method was confi rmed by analyzing standard samples and soil samples collected in districts of the Moscow region.
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Dyaninggar, Pratyangga Surya, Sri Juari Santosa, and Bambang Rusdiarso. "Preliminary Research on the Analysis of Chromium(III) Based on Digital Image Processing Method Utilizing Gold Nanoparticles as Colorimetric Sensor." Nano Hybrids and Composites 37 (August 31, 2022): 15–22. http://dx.doi.org/10.4028/p-t3ga7q.
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Gold nanoparticles (AuNPs) have been successfully synthesized using L-Ascorbic Acid as a reducing agent and p-Aminobenzoic Acid as a capping agent. These AuNPs were used to detect and quantify Chromium(III) as a pollutant utilizing its unique characteristic called surface plasmon resonance (SPR). The change of SPR and color of AuNPs then was employed to determine the concentration of Cr(III) by using colorimetric and Digital Image Colorimetric methods. Digital Image Colorimetry allowed to development of an easier and cheaper way to detect and quantify Cr(III). AuNPs capped p-aminobenzoic acid (AuNPs-PABA) were synthesized at room temperature without using any heating method. Then synthesized AuNPs-PABA were characterized using UV-Visible spectrometer and Transmission Electron Microscope. This AuNPs-PABA then got contacted to Cr(III) and color-changing happened in AuNPs-PABA was analyzed using UV-Vis Spectrometer and captured for Digital Image Colorimetric. The result showed that Digital Image Colorimetric method has a very big potential to be developed for AuNPs-based detection and quantification of Cr(III) as confirmed by good linearity of AuNPs-PABA-Cr(III) respect with Difference Intensity of Red Color Component, wider dynamic range, and Lower Limit of Detection (LoD).
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Xuan, Shi-Hai, Yu-Gui Zhou, Bo Shao, et al. "Enzymic colorimetry-based DNA chip: a rapid and accurate assay for detecting mutations for clarithromycin resistance in the 23S rRNA gene of Helicobacter pylori." Journal of Medical Microbiology 58, no. 11 (2009): 1443–48. http://dx.doi.org/10.1099/jmm.0.010785-0.
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Macrolide drugs, such as clarithromycin (CAM), are a key component of many combination therapies used to eradicate Helicobacter pylori. However, resistance to CAM is increasing in H. pylori and is becoming a serious problem in H. pylori eradication therapy. CAM resistance in H. pylori is mostly due to point mutations (A2142G/C, A2143G) in the peptidyltransferase-encoding region of the 23S rRNA gene. In this study an enzymic colorimetry-based DNA chip was developed to analyse single-nucleotide polymorphisms of the 23S rRNA gene to determine the prevalence of mutations in CAM-related resistance in H. pylori-positive patients. The results of the colorimetric DNA chip were confirmed by direct DNA sequencing. In 63 samples, the incidence of the A2143G mutation was 17.46 % (11/63). The results of the colorimetric DNA chip were concordant with DNA sequencing in 96.83 % of results (61/63). The colorimetric DNA chip could detect wild-type and mutant signals at every site, even at a DNA concentration of 1.53×102 copies μl−1. Thus, the colorimetric DNA chip is a reliable assay for rapid and accurate detection of mutations in the 23S rRNA gene of H. pylori that lead to CAM-related resistance, directly from gastric tissues.
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ZANUNCIO, ANTONIO JOSÉ VINHA, EMANUEL ARNONI COSTA, AMÉLIA GUIMARÃES CARVALHO, VINÍCIUS RESENDE DE CASTRO, ANGÉLICA DE CASSIA OLIVEIRA CARNEIRO, and SOLANGE DE OLIVEIRA ARAÚJO. "ARTIFICIAL INTELLIGENCE AND COLORIMETRY AS A COMBINED NON-DESTRUCTIVE METHOD TO PREDICT PROPERTIES OF HEAT-TREATED WOOD." Cellulose Chemistry and Technology 56, no. 9-10 (2022): 941–48. http://dx.doi.org/10.35812/cellulosechemtechnol.2022.56.84.
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Colorimetric evaluation is practical, accurate and fast. Starting from the generally established fact that a heat treatment changes the wood properties, the present paper aimed to predict the properties of heat-treated wood by using colorimetry and artificial neural networks (ANNs). Eucalyptus grandis and Pinus caribaea wood samples were heat-treated to evaluate their color, as well as physical and mechanical properties. The relationship between the wood color and its physical and mechanical properties was evaluated through multilayer perceptron (MLP) neural network. The heat treatment darkened the wood, increased its dimensional stability and reduced its mechanical resistance. Artificial neural networks based on colorimetric and temperature parameters were efficient in modeling the wood properties, with better results to predict its physical parameters. The coefficient of determination (R2) of the models was high and the root mean squared error (RMSE%) low – with homogeneous distribution. The findings suggest that colorimetry is adequate as a non-destructive tool to evaluate heat-treated wood.
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Kumpanenko, I. V., A. V. Roshchin, N. A. Ivanova, A. V. Bloshenko, N. A. Shalynina, and T. N. Korneeva. "Colorimetry: Choice of colorimetric parameters for chromophore concentration measurements." Russian Journal of General Chemistry 84, no. 11 (2014): 2295–304. http://dx.doi.org/10.1134/s1070363214110498.
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Korzhan, L., S. Kulichenko, and S. Lelyushok. "Smart Colorimetric Determination of Ionic Surfactants with Bromothymol Blue at the Presence of Nonionic Surfactant Triton X-100." Methods and Objects of Chemical Analysis 18, no. 3 (2023): 136–42. http://dx.doi.org/10.17721/moca.2023.136-142.
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The application of digital colorimetry for the determination of ionic surfactants, moderately hydrophobic medicinal substances of ionic nature in the system with bromothymol blue (BTB) in aqueous solutions using a smartphone has been shown. The influence of the solution nonionic surfactant Triton X-100 (TX-100) on the parameters of the colorimetric determination of bromothymol blue has been studied. It was established that the addition of TX-100 improves the linearity of the graduation dependence of the colorimetric signal of bromothymol blue (r ˃ 0.99) and reduces the detection limit of the dye by 15 %. The optimal conditions for the colorimetric determination of the cationic surface-active substance cetylpyridinium chloride (CPC), reagent concentration, and pH were established (LOD = 2.5·10-6 mol L-1, Sr ˂ 2 %). The spike recovery method checked the trueness of the results obtained in the model solutions analysis. The procedure for determining CPC in the drug "Septolete Total" was verified by the addition technique. The ability of a nonionic surface-active substance to stabilize the colloidal chemical state of solutions of hydrophobic counterions associates was used for the colorimetric determination of the anionic surfactant sodium dodecyl sulfate (r = 0.99; LOD = 7.5·10-7 mol L-1) and the less hydrophobic indomethacin (LOD = 6.6·10-6 mol L-1) with the BTB-TX-100-CPC reagent system.
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Marakaeva, Anastasia V., and Irina V. Kosyreva. "Visual Colorimetric and Colorimetric Determination of Cefuroxime." Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology 20, no. 1 (2020): 16–23. http://dx.doi.org/10.18500/1816-9775-2020-20-1-16-23.
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Garmay, Andrei V., Kirill V. Oskolok, Oksana V. Monogarova, and Stepan N. Chichulin. "Colorimetric determination of ammonium in soils samples." Lomonosov chemistry journal 66, no. 3, 2025 (2025): 206–12. https://doi.org/10.55959/msu0579-9384-2-2025-66-3-206-212.
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A technique for determining ammonium as an indophenolic dye in soil samples by digital colorimetry using household optical means of obtaining raster images is proposed. The developed approach is based on photometric methods for determining ammonium after extraction with potassium chloride from the soil. This technique provides accuracy and sensitive digital colorimetric techniques close to spectrophotometric ones. The adequacy of the proposed approach was confi rmed by analyzing standard samples and soil samples collected in the districts of the Moscow region.
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Du, Bin, Shu Geng, Wei Cao, et al. "Investigation of the Colorimetric Characteristics of VX in Squaraine-Based Solutions." Chemosensors 11, no. 2 (2023): 137. http://dx.doi.org/10.3390/chemosensors11020137.
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Colorimetry is an important on-site detection method for organophosphorus compounds. O-Ethyl S-(2-diisopropylaminoethyl) methylphosphonothioate (VX) is recognized as one of the deadliest organophosphorus chemical agents, and the rapid on-site detection of VX is of great significance to public safety. In this paper, a squaraine derivative was synthesized as probe molecules, and the sensing characteristics of VX in a colorimetric solution system containing tetrabutylammonium fluoride (TABF) were studied with UV−Vis spectroscopy, nuclear magnetic resonance (1H NMR), and mass spectrometry. The results showed that the binding of the thiol moiety of VX to the quaternary ring of the squaraine probe changed the molecular conjugation system, and that the rapid colorimetric detection of micro-trace VX was achieved based on color change before and after interaction with squaraine, enabling the detection limit of VX to be as low as 0.4 μg/mL. Moreover, the colorimetry method also possessed satisfactory sensitivity and could detect VX from other organophosphorus pesticides (e.g., parathion and dichlorvos), phosphorus-containing reagents (e.g., diethyl chlorophosphate and dimethyl methylphosphonate), a benzene series (e.g., toluene), and acid and base agents (e.g., acetic acid and triethylamine, respectively), which demonstrated that squaraine-based colorimetry could provide fast, on-site measurement results for VX detection. The strategy of this research could be extended as a common approach for the detection of other organophosphorus nerve agents or organophosphorus pesticides.
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Guerreiro, Julio César, Ana Paula Azevedo, Evandro Pereira Prado, et al. "CASSAVA ROOTS DAMAGED BY MIGDOLUS FRYANUS PRODUCE POOR QUALITY STARCH." REVISTA DE AGRICULTURA NEOTROPICAL 9, no. 2 (2022): e6884. http://dx.doi.org/10.32404/rean.v9i2.6884.
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The aim of this study was to evaluate the quality of starch produced by plants that suffer damage caused by M. fryanus, analyzed by colorimetric and morphological indices. Colorimetric data were composed of luminosity indices, “a” and “b” chroma coordinates and darkening index. For morphological data, the perimeter and area of starch granules extracted from cassava roots were analyzed. The variables were summarized in a principal component analysis and the axes scores retained for interpretation were correlated with the damage caused by the M. fryanus. From these analyses and observing the main effects, linear regression models for the variables were determined, according to the level of damage caused by the pest. The damage caused by M. fryanus in the roots strongly influenced the indices related to starch colorimetry, hence, under conditions of greater damage, the tendency of the chroma coordinates to vary toward red and yellow was observed and a decrease in starch luminosity occurred, inversely proportional to the increase in darkening. Similar to what was observed in the colorimetric data, a drop in the size of starch granules was noted in the roots, which suffered greater damage from the pest.
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Kanakashree, H. R., T. N. Kishora, and T. N. Ramesh. "Colorimetric Studies for the Detection of Dopamine Using Vanillin." Oriental Journal Of Chemistry 40, no. 6 (2024): 1768–73. https://doi.org/10.13005/ojc/400629.
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Complex formation between dopamine hydrochloride and vanillin using a colorimetric method was investigated to develop a simple and effective approach for the detection and quantification of dopamine. Dopamine, a neurotransmitter with significant biological importance, reacts with vanillin, an aromatic aldehyde in ethanol, resulting in a color change (colorless to light brown) that was measured using colorimetry. The optimal conditions for complex formation with a specific molar ratio of dopamine hydrochloride to vanillin have been studied to establish a calibration curve correlating absorbance with dopamine hydrochloride concentration, allowing for the accurate quantification of dopamine hydrochloride. This colorimetric method provides a cost-effective, accessible, and reliable means of detecting dopamine, with potential applications in clinical diagnostics, pharmaceutical analysis, and neurological research. The above method offers a quantitative way to determine up to 4 ppm levels of dopamine hydrochloride.
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Vucāne, Sanita, Ingmars Cinkmanis, and Mārtiņš Šabovics. "Colorimetric Measurements of Vegetable Oils by Smartphone-Based Image Analysis." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 76, no. 1 (2022): 110–15. http://dx.doi.org/10.2478/prolas-2022-0017.
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Abstract One of the most important indicators of quality of vegetable oils is colour, which can be detected with colorimetric measurements. The determination of colour is traditionally done using colorimeters, spectrometers, tintometers, and other analytical equipment. As an alternative to replace the classical analytical methods, smartphone-based colorimetry using digital image analysis can be used. For colorimetric detection of colour in vegetable oils, a Huawei P30 lite smartphone and android application “Colour Picker” with an image matching algorithm RGB model was used. The image of sample and standard solutions was captured in a polyvinyl chloride box with light-emitting diode (LED) lamps. The aim of the study was to detect the colour of vegetable oils with smartphone-based image analysis. The detected colour of eleven vegetable oils (sea buckthorn, sunflower, rice, macadamia nut, hemp, corn, grape, linseed, rapeseed, olive, and milk thistle oils) was compared with standard solutions of iodine with a concentration range from 0 to 100 mg·100 ml−1. The results show that smartphone-based colorimetry can be used for detection of the colour of vegetable oils and it is possible to compare the colour with standard solutions of iodine. The colour of vegetable oils was expressed as the colour number obtained from an iodine standard solution prepared in deionised water.
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Harpaz, Dorin, Evgeni Eltzov, Timothy S. E. Ng, Robert S. Marks, and Alfred I. Y. Tok. "Enhanced Colorimetric Signal for Accurate Signal Detection in Paper-Based Biosensors." Diagnostics 10, no. 1 (2020): 28. http://dx.doi.org/10.3390/diagnostics10010028.
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Paper-based colorimetric biosensors combine the use of paper with colorimetric signal detection. However, they usually demonstrate lower sensitivities because a signal amplification procedure has not been used. Stopping the reaction of colorimetric signal generation is often used in lab-based assays in order to amplify and stabilize the colorimetric signal for detection. In this study, the generation of a stopped colorimetric signal was examined for accurate and enhanced signal detection in paper-based biosensors. The colorimetric reaction in biosensors is usually based on the interaction between the enzyme horseradish peroxidase (HRP) and a selected chromogenic substrate. The two most commonly used HRP substrates, 3,3’,5,5’-tetramethylbenzidine (TMB) and 2’-azinobis (3-ethylbenzothiazoline-6-sulfonic-acid) (ABTS), were compared in terms of their ability to generate a stopped colorimetric signal on membrane. The stopped colorimetric signal was visible for TMB but not for ABTS. Moreover, the generation of stopped colorimetric signal was dependent on the presence of polyvinylidene-difluoride (PVDF) membrane as the separation layer. With PVDF the colorimetric signal (color intensity) was higher (TMB: 126 ± 6 and ABTS: 121 ± 9) in comparison to without PVDF (TMB: 110 ± 2 and ABTS: 102 ± 4). The TMB stopped colorimetric signal demonstrated a more stable signal detection with lower standard deviation values. To conclude, a stopped colorimetric signal can be generated in paper-based biosensors for enhanced and accurate signal detection.
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Parker, Gregory B., Olin D. Smith, and W. James Grichar. "Colorimetric Assessment of Pod Disease in Peanuts: Comparison with Visual Methods and Efficacy of Use in Selection1." Peanut Science 16, no. 2 (1989): 76–82. http://dx.doi.org/10.3146/i0095-3679-16-2-5.
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Abstract Colorimetry was evaluated as a method to assess pod disease in peanuts (Arachis hypogaea L.) caused primarily by Pythium myriotylum Drechs., Sclerotium rolfsii Sacc., and Rhizoctonia solani Kuhn. Data analyzed were from nineteen replicated tests conducted from 1982 to 1987, exclusive of 1985, in three South Texas locations. Each plot was scored for pod disease colorimetrically and visually. A negative linear relationship (R2>96%) was found between Hunter color values (L and b) and percent infection measured visually for samples hand selected to approximate eleven disease levels varying from 0-100%. Variability among readings was less at extremes of infection. Correlation both between visual ratings and between visual and colorimetric ratings was affected by soil differences, pathogens infecting the pods, pod genotype, and level of infection present. Correlation among visual raters was generally higher than that between color value ratings. Two-thirds of the lines in these tests classified visually as being in the best 50% for pod disease were also in the best 50% according to colorimetric scores. Use of colorimetry in conjunction with a single visual rating was estimated to increase efficiency and reduce costs of evaluation compared to multiple visual ratings.
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Layne, Tiffany R., Anchi Scott, Larissa L. Cunha, Rachelle Turiello, and James P. Landers. "Three-Dimensional-Printed Instrument for Isothermal Nucleic Acid Amplification with Real-Time Colorimetric Imaging." Micromachines 15, no. 2 (2024): 271. http://dx.doi.org/10.3390/mi15020271.
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Isothermal amplification methods have become popular in research due to the simplicity of the technology needed to run the reactions. Specifically, loop-mediated isothermal amplification (LAMP) has been widely used for various applications since first reported in 2000. LAMP reactions are commonly monitored with the use of colorimetry. Although color changes associated with positive amplification are apparent to the naked eye, this detection method is subjective due to inherent differences in visual perception from person to person. The objectivity of the colorimetric detection method may be improved by programmed image capture over time with simultaneous heating. As such, the development of a novel, one-step, automated, and integrated analysis system capable of performing these tasks in parallel is detailed herein. The device is adaptable to multiple colorimetric dyes, cost-effective, 3D-printed for single-temperature convective heating, and features an easy-to-use LabVIEW software program developed for automated image analysis. The device was optimized and subsequently validated using four messenger-RNA targets and mock forensic samples. The performance of our device was determined to be comparable to that of a conventional thermal cycler and smartphone image analysis, respectively. Moreover, the outlined system is capable of objective colorimetric analysis, with exceptional throughput of up to 96 samples at once.
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Park, Seo Yeon, Rajamanickam Sivakumar, and Nae Yoon Lee. "D-Glucose-Mediated Gold Nanoparticle Fabrication for Colorimetric Detection of Foodborne Pathogens." Biosensors 14, no. 6 (2024): 284. http://dx.doi.org/10.3390/bios14060284.
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Gold nanoparticle (AuNP) fabrication via the oxidation of D-glucose is applied for detecting two foodborne pathogens, Enterococcus faecium (E. faecium) and Staphylococcus aureus (S. aureus). D-glucose is used as a reducing agent due to its oxidation to gluconic acid by sodium hydroxide (NaOH), resulting in the formation of AuNPs. Based on this mechanism, we develop AuNP-based colorimetric detection in conjunction with loop-mediated isothermal amplification (LAMP) for accurately identifying the infectious bacteria. Here, Au+ ions bind to the base of double-stranded DNA. In the presence of D-glucose and NaOH, the LAMP amplicon-Au+ complex maintains its bound state at 65 °C for 10 min while it is reduced to AuNPs in a dispersed form, exhibiting a red color. We aimed to pre-mix D-glucose with LAMP reagents before amplification and induce successful colorimetry without inhibiting amplification to simplify the experimental process and decrease the reaction time. Therefore, the entire process, including LAMP and colorimetric detection, is accomplished in approximately 1 h. The limit of detection of E. faecium and S. aureus is confirmed using the introduced method as 101 CFU/mL and 100 fg/μL, respectively. We expect that colorimetric detection using D-glucose-mediated AuNP synthesis offers an application for simple and immediate molecular diagnosis.
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Gibbs, Harold A. A., and Leonard W. O'Garro. "Capsaicin Content of West Indies Hot Pepper Cultivars Using Colorimetric and Chromatographic Techniques." HortScience 39, no. 1 (2004): 132–35. http://dx.doi.org/10.21273/hortsci.39.1.132.
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Colorimetric and chromatographic methods were used to assess capsaicinoid levels in a pungent Caribbean-grown pepper collection comprising 28 accessions of Capsicum chinense and one each of C. annuum and C. frutescens. Two colorimetric methods, one commonly used and attributed to Bajaj (1980) and a modification of the Bajaj method were also compared for congruity and ease of use. Capsaicin content of the cultivars ranged from 37.6 to 497.0 mg/100 g in ripe fruits and 27.8 to 404.5 mg/100 g in green fruit, as determined by Bajaj's method. The corresponding Scoville units of pungency varied from 15,000 to 300,000 for ripe fruit and 7,500 to 270,000 for green fruit. Levels of capsaicin assessed by the modified Bajaj method varied from 15.0 to 402.4 mg/100 g and 13.7 to 356.4 mg/100 g in ripe and green fruit, respectively. On the basis of capsaicin levels assessed by each colorimetric method, the pepper cultivars were differentiated into seven distinct pungency groups. For each method, similar groupings of cultivars were observed for ripe and green fruit and groups of the same numerical designation were mainly comprised of common assessions. These results indicate that the two colorimetric methods generally agree. In contrast, the modified colorimetric method was more efficient than Bajaj's procedure, which required pretreatment of pepper extracts to remove the extracting solvent by evaporation and interfering chromogenic pigments by column chromatography. Phase separation of capsaicin and interfering pigments in pepper extracts by use of dilute acid was the only pretreatment required in the modified Bajaj method before colorimetry. High performance liquid chromatography performed on fruit extracts of the cultivars revealed the presence of the capsaicinoids capsaicin, homocapsaicin, dihydrocapsaicin, nordihydrocapsaicin, and homodihydrocapsaicin. Capsaicin and homocapsaicin were detected in greater abundance than dihydrocapsaicin and nordihydrocapsaicin in fruit of all cultivars. Homodihydrocapsaicin was the least abundant of the capsaicinoids and was generally absent in ripe fruit.
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Chaplan, Cory A., Haydn T. Mitchell, and Andres W. Martinez. "Paper-based standard addition assays." Anal. Methods 6, no. 5 (2014): 1296–300. http://dx.doi.org/10.1039/c4ay00205a.
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Standard addition assays conducted on paper-based microfluidic devices are introduced as an alternative to external standards for calibrating quantitative tests. To demonstrate this technique, a colorimetric, paper-based, standard addition assay was optimized for the determination of glucose concentrations in the range of 0 to 5 mM. Comparable results were obtained from the assay via digital image colorimetry under three different lighting conditions.
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Audrynachristie, Brigitta Stella, Mochammad Amin Alamsjah, and Kismiyati. "Use of Fruit Waste as Natural Dyes in pH-Sensitive Colorimetric Sensors for Tilapia Fillets Quality Decline." Jurnal Ilmiah Perikanan dan Kelautan 17, no. 2 (2025): 512–21. https://doi.org/10.20473/jipk.v17i2.57245.
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Graphical Abstract Highlight Research 1. The use of dragon fruit peel, mangosteen peel and onion peel waste as natural dyes in colorimetric sensors was analyzed in this study. 2. Natural dyes are added to bioplastics with kappa carrageenan polymer, a mixture of corn starch and kappa carrageenan, and corn starch to determine the characteristics of the best bioplastic to be used as a colorimetry sensor. 3. The quality of the fish fillet compared to the color changes in colorimetry sensor was analysed in this study. 4. Mixture of kappa carrageenan and corn starch as bioplastic with casting method was analyzed in this study. Abstract Tilapia fillet aimed to prolonging shelf life, may still experience quality deterioration posing food safety risks. Colorimetric pH indicators offer a simple and affordable solution to the food industry to evaluating fish spoilage. Natural dyes reduce potential health risks associated with synthetic dyes. Anthocyanin sources which haven’t been explored like dragon fruit rind, mangosteen rind, and red onion skin serve as real time quality and safety assessment tools for consumers. This study explores the potential of anthocyanin extracted from dragon fruit rind, mangosteen rind, and red onion skin as colorimetric sensors for evaluating the quality and safety of tilapia fillets. The anthocyanin-based sensors developed using kappa carrageenan and corn starch polymers to achieve sustainability fisheries program and were characterized according to the Japanese Industrial Standard for bioplastics. The results show that anthocyanin from dragon fruit skin exhibited the best color change in response to pH changes in the tilapia fillet, indicating its potential as a reliable indicator of spoilage. This research highlights the feasibility of using natural dyes as colorimetric sensors, reducing the risk of health hazards associated with synthetic dyes. This study also shows that different polymers give different characteristic of bioplastic. Carrageenan bioplastic shows best thickness values of 0,118 mm; Carrageenan and corn starch bioplastics show best tensile strength values of 20,62 MPa; Carrageenan bioplastic shows best elongation values of 254%; and all polymers show the same biodegradation rate values of 14,29%. Further studies are needed to explore other natural dyes and optimize the polymers for optimal bioplastic characteristics.
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Khoroshailo, Yurii, Iryna Sezonova, Oleksandr Degtiarov, Maksym Korbetskyy, and Pavlo Biletskyy. "RESEARCH ON THE POSSIBILITIES OF USING COLORIMETRY IN MEDICINE." Metrology and instruments, no. 2 (December 13, 2024): 07. https://doi.org/10.30837/2663-9564.2024.2.07.
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Studies of the possibility of using colorimetry in medicine aim to use modern methods and devices for diagnosing the concentration of substances in natural solutions. Colorimetry as a method of measuring the concentration of substances is based on the ability of solutions of certain substances to change color at different concentrations. The method allows quantitatively determining the concentration of a substance based on the color intensity recorded by a device - a colorimeter. The basic principles of colorimetry include spectrophotometry, application of the Bouguer-Lambert-Beer law, calibration of the device, selection of wavelength, assessment of light absorption and comparison with standards. The paper presents the main aspects and principles of colorimetric analysis, which are important for obtaining accurate results.
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Mao, Xinxin, Yulong Wang, Lan Jiang, et al. "A Polydopamine-Coated Gold Nanoparticles Quenching Quantum Dots-Based Dual-Readout Lateral Flow Immunoassay for Sensitive Detection of Carbendazim in Agriproducts." Biosensors 12, no. 2 (2022): 83. http://dx.doi.org/10.3390/bios12020083.
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In this study, a fluorometric and colorimetric dual-readout lateral flow immunoassay (LFIA) using antibody functionalized polydopamine-coated gold nanoparticles (Au@PDAs) as a probe was developed for the detection of carbendazim (CBD). Colloidal gold nanoparticles (AuNPs) were coated with polydopamines (PDA) by the oxidation of dopamine to synthesize Au@PDA nanoparticles. The Au@PDA nanoparticles mediated ZnCdSe/ZnS quantum dots (QDs) fluorescence quenching and recovery, resulting in a reverse fluorescence enhancement detection format of CBD. The CBD detection was obtained by the competition between the CBD and the immobilized antigen for Au@PDAs labelled antibody binding, resulting in a significant fluorescence increase and colorimetry decrease corresponded to the concentration of CBD. Dual readout modes were incorporated into the LFIA using the colorimetry signal under natural light and the fluorescence signal under UV light. The cut-off value in the mode of the colorimetric signal and fluorometric signal for CBD detection was 0.5 μg/mL and 0.0156 μg/mL, respectively. The sensitivity of LFIA of the fluorescence mode was 32 times higher than that of the colorimetry mode. There was negligible cross reactivity obtained by using LFIA for the determination of thiabendazole, benomyl, thiophanate-methyl, and thiophanate-ethyl. Consistent and satisfactory results have been achieved by comparing the results of Au@PDAs-QDs-LFIA and liquid chromatography-tandem mass spectrometry (LC—MS/MS) testing spiked cucumber and strawberry samples, indicating good reliability of the Au@PDAs-QDs-LFIA.
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Bello, Thaísa B., Anderson G. Costa, Thainara R. da Silva, Juliana L. Paes, and Marcus V. M. de Oliveira. "Tomato quality based on colorimetric characteristics of digital images." Revista Brasileira de Engenharia Agrícola e Ambiental 24, no. 8 (2020): 567–72. http://dx.doi.org/10.1590/1807-1929/agriambi.v24n8p567-572.
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ABSTRACT Results of evaluations using optical evaluation methods may be correlated with tomato quality and maturation. In this context, the objective of this study was to evaluated the correlation between tomato colorimetric and physico-chemical variables, clustering them as a function of maturation stages, using multivariate analysis. The experiment was conducted using 150 fruits and three maturation stages (immature, light red and mature). The physico-chemical variables were evaluated through traditional methods. The colorimetric variables were assessed on images in RGB color model taken with a digital camera. The correlation between colorimetric and physico-chemical variables was analyzed using the Pearson’s coefficient. Principal components analysis and k-means clustering method was applied to three data set: RGB isolated variables; colorimetric variables calculated by relation between the RGB bands (colorimetric indexes); and physico-chemical variables. The colorimetric variables present higher explanatory capacity of the maturation variation than physico-chemical variables. The colorimetric indexes presented higher performance in clustering (accuracy of 0.98) tomatoes as a function of maturation.
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Mo, Manni, Bo Fu, Piyush Hota, et al. "Threshold-Responsive Colorimetric Sensing System for the Continuous Monitoring of Gases." Sensors 23, no. 7 (2023): 3496. http://dx.doi.org/10.3390/s23073496.
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Colorimetric sensors are widely used because of their inherent advantages including accuracy, rapid response, ease-of-use, and low costs; however, they usually lack reusability, which precludes the continuous use of a single sensor. We have developed a threshold-responsive colorimetric system that enables repeated analyte measurements by a single colorimetric sensor. The threshold responsive algorithm automatically adjusts the sensor exposure time to the analyte and measurement frequency according to the sensor response. The system registers the colorimetric sensor signal change rate, prevents the colorimetric sensor from reaching saturation, and allows the sensor to fully regenerate before the next measurement is started. The system also addresses issues common to colorimetric sensors, including the response time and range of detection. We demonstrate the benefits and feasibility of this novel system, using colorimetric sensors for ammonia and carbon dioxide gases for continuous monitoring of up to (at least) 60 detection cycles without signs of analytical performance degradation of the sensors.
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Labadie, Michael P., and Charles E. Boufford. "Gas Chromatographic Assay of Supplemental Vitamin E Acetate Concentrates: Collaborative Study." Journal of AOAC INTERNATIONAL 71, no. 6 (1988): 1168–71. http://dx.doi.org/10.1093/jaoac/71.6.1168.
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Abstract A gas chromatographic (GC) method for determination of supplemental a-tocopheryl acetate in high potency vitamin £ powders and oils was collaboratively studied as an alternative to the AOAC colorimetric method (43.147-43.151, Emmerie-Engel), which requires saponification, extraction of the saponifiable fraction, and quantitation by colorimetry. The simpler GC procedure requires only extraction and/or enzymatic digestion and dilution before quantitation. Six blind duplicates were distributed to 10 laboratories; all 10 returned results. Repeatability (sr) and reproducibility (sR), % vitamin E/g, for the feed oil concentrates was 1.1 and 1.3, respectively; for the feed adsorbates 1.0 and 1.5; and for the spray-formulated powders 1.4 and 1.3. These results compare favorably with results obtained in a comparison study of the GC and Emmerie-Engel methods conducted by BASF in 1985. The method has been approved interim official first action for determination of a-tocopheryl acetate in vitamin E acetate concentrates as an alternative for those products only to AOAC colorimetric method 43.147-43.151
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Gavrilenko, Nataliya A., Nadezda V. Saranchina, Aleksey V. Sukhanov, Mikhail A. Gavrilenko, and Elena V. Zenkova. "Colorimetric Polymethacrylate Sensor." Advanced Materials Research 880 (January 2014): 19–24. http://dx.doi.org/10.4028/www.scientific.net/amr.880.19.
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The paper describes a new colorimetric sensor. The colorimetric sensors were made of optically transparent polymethacrylate matrix with physically immobilized analytical reagent which is responsible for the extraction of the analyte into the sensing material and changing its color. The developed colorimetric sensor can be used in determination of various analytes using both solid-phase spectrophotometer and naked eye.
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Liu, Xiaoting, Dan Wu, Abbas Ali Abid, Ying Liu, Jianfeng Zhou, and Qichun Zhang. "Determination of Paddy Soil Ammonia Nitrogen Using Rapid Detection Kit Coupled with Microplate Reader." Toxics 10, no. 12 (2022): 725. http://dx.doi.org/10.3390/toxics10120725.
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Indophenol blue colorimetry has been widely used for determining soil ammonia nitrogen, but this method has some disadvantages, such as complex reagent preparation, high toxicity, and long colorimetric time. Hence, we aimed to develop a rapid soil ammonia nitrogen determination method using a rapid detection kit. In order to select a suitable extractant, different concentrations of KCl and NaCl solutions were used to extract soil. The ammonia nitrogen content in different types of soils was determined using a rapid detection kit (purchased from Zhejiang Luheng Environmental Technology Limited Company) coupled with a microplate reader. The kit method was compared with the traditional indophenol blue colorimetry method. The results showed no significant difference between the 1 mol·L−1 KCl extraction kit method and indophenol blue colorimetry (p > 0.05). The linearity of the working curve was smooth, the linear detection range was 0.0–2.00 mg·L−1, the average relative standard deviation was 7.00% (n = 5), the standard addition recovery rate was 89.31–118.23%, and the detection limit were was 0.074 mg·L−1. We concluded that the 1 mol·L−1 KCl extraction kit method can be applied to determine the ammonia nitrogen content of paddy soil with different chemical properties. The 1 mol·L−1 KCl extraction kit method has the advantage over indophenol blue colorimetry due to its simple reagent preparation, convenient operation, and shorter detection time (the coloring and colorimetric time for 96 samples was only 30 min using the kit method coupled with a microplate reader). Hence, it has the potential for application due to its rapid determination of soil samples in large quantities.
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Zhang, Liang, Huai Xiu Lu, Long Quan Shao, et al. "Soak Colored Zirconia Ceramics and its Colorimetric Plate." Key Engineering Materials 492 (September 2011): 362–65. http://dx.doi.org/10.4028/www.scientific.net/kem.492.362.
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Objective: To test specimens of soak-colored Vita In-Ceram YZ zirconia ceramics and the colorimetric data of staining solution colorimetric plate. Methods: five sets of specimens were prepared, soaking and coloring them with LL1 to LL5 staining solution for 2 min respectively. Specimens were sintered for 2 h at 1530°C, and grinded one side of the specimens to 1.5 mm in thickness. The colors of the specimens and staining solution colorimetric plate were tested by spectrophotometer in black background. The chromatic aberration between the specimens and colorimetric plate were calculated as well as measured the lightness difference, hue angle difference, and saturation difference. Results: the colorimetric data of the dyed specimens are L: 59.81 ~ 78.93; a: 0.36 ~ 9.36; b: 19.62 ~ 26.91. The color space of the specimens and staining solution colorimetric plate was similar and the chromatic aberration was 4.02-6.96 NBS units. The biggest difference between them was the hue angle difference, mean 7.38, followed by the saturation difference, mean 2.31; minimum lightness difference, mean 2.29. Conclusion: staining solution colorimetric plate was suitable to be colorimetric reference for the color-matching of the basic layer of the ceramic. Proper chromatic aberration leaves color revision space for the translucent porcelain veneer.
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Ahmad, Noor Azizah, Lee Yook Heng, Faridah Salam, Mohd Hazani Mat Zaid, and Sharina Abu Hanifah. "A Colorimetric pH Sensor Based on Clitoria sp and Brassica sp for Monitoring of Food Spoilage Using Chromametry." Sensors 19, no. 21 (2019): 4813. http://dx.doi.org/10.3390/s19214813.
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A developed colorimetric pH sensor film based on edible materials for real-time monitoring of food freshness is described. The mixed natural dyes from edible plants Clitoria sp and Brassica sp were extracted and incorporated into ι-carrageenan film as a colorimetric pH sensor film for monitoring food spoilage and its freshness. The color changes of the developed colorimetric sensor film were measured with chromametry and UV-vis spectroscopy, respectively. Experimental results show that colorimetric pH sensor film demonstrated statistically significant differences (p < 0.05) between CIE-L*a*b* coordinates color system indicated that the developed colorimetric sensor film was able to give a gradual change in color over a wide pH range. The color of the colorimetric sensor film also changes discretely and linearly with factors that contribute to food spoilage using shrimp and durian samples. Moreover, the developed colorimetric pH sensor film has the potential to be used as a safe, non-destructive testing and also a flexibly visual method for direct assessment of food freshness indicator during storage.
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Lee, Yubeen, Izzati Haizan, Sang Baek Sim, and Jin-Ha Choi. "Colorimetric Biosensors: Advancements in Nanomaterials and Cutting-Edge Detection Strategies." Biosensors 15, no. 6 (2025): 362. https://doi.org/10.3390/bios15060362.
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Colorimetric-based biosensors are practical detection devices that can detect the presence and concentration of biomarkers through simple color changes. Conventional laboratory-based tests are highly sensitive but require long processing times and expensive equipment, which makes them difficult to apply for on-site diagnostics. In contrast, the colorimetric method offers advantages for point-of-care testing and real-time monitoring due to its flexibility, simple operation, rapid results, and versatility across many applications. In order to enhance the color change reactions in colorimetric techniques, functional nanomaterials are often integrated due to their desirable intrinsic properties. In this review, the working principles of nanomaterial-based detection strategies in colorimetric systems are introduced. In addition, current signal amplification methods for colorimetric biosensors are comprehensively outlined and evaluated. Finally, the latest trends in artificial intelligence (AI) and machine learning integration into colorimetric-based biosensors, including their potential for technological advancements in the near future, are discussed. Future research is expected to develop highly sensitive and multifunctional colorimetric methods, which will serve as powerful alternatives for point-of-care testing and self-testing.
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Luo, Cheng, Wei Wen, Fenge Lin, Xiuhua Zhang, Haoshuang Gu, and Shengfu Wang. "Simplified aptamer-based colorimetric method using unmodified gold nanoparticles for the detection of carcinoma embryonic antigen." RSC Advances 5, no. 15 (2015): 10994–99. http://dx.doi.org/10.1039/c4ra14833a.
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A new colorimetric aptasensor for the detection of CEA was developed. Aptamer-based colorimetric method with nanoparticles was used for the detection of CEA. The colorimetric aptasensor has potential for the detection of other proteins or nucleotides.
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Gardner, R. A. "Colorimetric flow visualization." Experiments in Fluids 3, no. 1 (1985): 33–34. http://dx.doi.org/10.1007/bf00285268.
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Wu, Yu, Jing Feng, Guang Hu, En Zhang, and Huan-Huan Yu. "Colorimetric Sensors for Chemical and Biological Sensing Applications." Sensors 23, no. 5 (2023): 2749. http://dx.doi.org/10.3390/s23052749.
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Colorimetric sensors have been widely used to detect numerous analytes due to their cost-effectiveness, high sensitivity and specificity, and clear visibility, even with the naked eye. In recent years, the emergence of advanced nanomaterials has greatly improved the development of colorimetric sensors. This review focuses on the recent (from the years 2015 to 2022) advances in the design, fabrication, and applications of colorimetric sensors. First, the classification and sensing mechanisms of colorimetric sensors are briefly described, and the design of colorimetric sensors based on several typical nanomaterials, including graphene and its derivatives, metal and metal oxide nanoparticles, DNA nanomaterials, quantum dots, and some other materials are discussed. Then the applications, especially for the detection of metallic and non-metallic ions, proteins, small molecules, gas, virus and bacteria, and DNA/RNA are summarized. Finally, the remaining challenges and future trends in the development of colorimetric sensors are also discussed.
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Quispe-Quispe, Arturo, Liz N. Huamani Navarro, and Ruth M. Ccopa Flores. "Colorimetric and Textile Properties of Huacaya Alpaca Fibers Dyed With Cochineal: A Sustainable Approach." Revista de Gestão Social e Ambiental 19, no. 1 (2025): e011033. https://doi.org/10.24857/rgsa.v19n1-150.
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Objective: To investigate the colorimetric and textile properties of Huacaya alpaca fibers dyed with cochineal, promoting a sustainable approach in the textile industry through the use of natural resources and traditional methods, improving product quality and minimizing environmental impact. Theoretical Framework: The main concepts and theories that support the research on the colorimetric and textile properties of Huacaya alpaca fibers dyed with cochineal dye are presented, focusing on sustainability. Method: The methodology included a randomized experimental design with a factorial arrangement to evaluate the impact of different mordants and concentrations of cochineal dye on the colorimetric and textile properties of alpaca fiber, with data collection through experimental methods. Results and Discussion: The results showed significant differences in colorimetry parameters, textile characteristics, and chemical bands, depending on the type of mordant and dye concentration used. Wash fastness was notably high in the MAL-2 and MCOL-2 samples, indicating the effectiveness of sustainable practices. However, the study's limitations might be related to the experimental design, sample selection, and analysis method employed. Research Implications: The results promote the use of natural dyes in textile production, encouraging sustainable practices, reducing harmful chemicals, and conserving biological resources, which influences conservation policies and supports fair trade and local products. Originality/Value: This study applies innovative methodologies to evaluate Huacaya alpaca fibers dyed with cochineal, promoting sustainability, the use of natural dyes, conservation of biological resources, fair trade, and sustainable management policies.
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Cabral, Isabel, Amanda Schuch, and Fernanda Steffens. "Color Biomimetics in Textile Design: Reproduction of Natural Plant Colors through Instrumental Colorant Formulation." Journal of Imaging 10, no. 7 (2024): 150. http://dx.doi.org/10.3390/jimaging10070150.
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This paper explores the intersection of colorimetry and biomimetics in textile design, focusing on mimicking natural plant colors in dyed textiles via instrumental colorant formulation. The experimental work was conducted with two polyester substrates dyed with disperse dyes using the exhaustion process. Textiles dyed with different dye colors and concentrations were measured in a spectrophotometer and a database was created in Datacolor Match Textile software version 2.4.1 (0) with the samples’ colorimetric properties. Colorant recipe formulation encompassed the definition and measurement of the pattern colors (along four defined natural plants), the selection of the colorants, and the software calculation of the recipes. After textile dyeing with the lowest expected CIELAB color difference (ΔE*) value recipe for each pattern color, a comparative analysis was conducted by spectral reflectance and visual assessment. Scanning electron microscopy and white light interferometry were also used to characterize the surface of the natural elements. Samples dyed with the formulated recipe attained good chromatic similarity with the respective natural plants’ colors, and the majority of the samples presented ΔE* between 1.5 and 4.0. Additionally, recipe optimization can also be conducted based on the colorimetric evaluation. This research contributes a design framework for biomimicking colors in textile design, establishing a systematic method based on colorimetry and color theory that enables the reproduction of nature’s color palette through the effective use of colorants.
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Oh, Hyun Ju, Byeong Jin Yeang, Young Ki Park, et al. "Washable Colorimetric Nanofiber Nonwoven for Ammonia Gas Detection." Polymers 12, no. 7 (2020): 1585. http://dx.doi.org/10.3390/polym12071585.
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The colorimetric sensor is a facile, cost-effective, and non-power-operated green energy material for gas detection. In this study, the colorimetric sensing property of a meta-aramid/dye 3 nanofiber sensor for ammonia (NH3) gas detection was investigated. This colorimetric sensor was prepared using various dye 3 concentrations via electrospinning. Morphological, thermal, structural, and mechanical analyses of the sensor were carried out by field-emission scanning electron microscopy, thermogravimetric analysis, Fourier-transform infrared spectroscopy, and a universal testing machine, respectively. A homemade computer color matching machine connected with a gas flow device characterized the response of the meta-aramid/dye 3 nanofiber colorimetric sensor to various exposure levels of NH3 gas. From the results, we confirmed that this colorimetric green energy sensor could detect ammonia gas in the concentration of 1–10 ppm with a sensing response time of 10 s at room temperature. After washing with laundry detergent for 30 min, the colorimetric sensors still exhibited sensing property and reversibility.
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Liu, Lin, Yuanqiang Hao, Dehua Deng, and Ning Xia. "Nanomaterials-Based Colorimetric Immunoassays." Nanomaterials 9, no. 3 (2019): 316. http://dx.doi.org/10.3390/nano9030316.
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Colorimetric immunoassays for tumor marker detection have attracted considerable attention due to their simplicity and high efficiency. With the achievements of nanotechnology and nanoscience, nanomaterials-based colorimetric immunoassays have been demonstrated to be promising alternatives to conventional colorimetric enzyme-linked immunoassays. This review is focused on the progress in colorimetric immunoassays with the signal amplification of nanomaterials, including nanomaterials-based artificial enzymes to catalyze the chromogenic reactions, analyte-induced aggregation or size/morphology change of nanomaterials, nanomaterials as the carriers for loading enzyme labels, and chromogenic reactions induced by the constituent elements released from nanomaterials.
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Lee, Sang Yoon, Hyo Je Park, Cheol Woo Han, Seung Yun Lee, and Gi Young Kweon. "Validation of a Rapid Colorimetric Method with Field Soils." Transactions of the ASABE 60, no. 4 (2017): 1271–78. http://dx.doi.org/10.13031/trans.12204.
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Abstract. Regularly monitoring the variations in soil composition is an essential part of proper cultivation management. Some conventional soil characterization methods are often costly and require experienced labor. The spectroscopic method is a simpler procedure that minimizes experimental steps and is faster and more economical than conventional methods. Wide-ranging analytical methods have been developed to determine soil phosphorus concentrations in the field; however, no method has been commercialized. Analytical methods based on colorimetric Mehlich-3 and Lancaster soil tests, developed to reduce measurement time in laboratories in the U.S. and South Korea, have been efficient. However, due to laboratory procedures, the soil was pretreated and not verified with field soil. We tested the rapid colorimetric method, which is for on-site plant-available soil phosphorus tests in the field and allows reduced analysis time. The target accuracy of the field tests was &gt;70% correlation with laboratory test results. This method consists of three steps: fast extraction, fast filtration, and fast color development. In a comparison of the rapid colorimetric method and the standard laboratory procedure, the R2 values were 0.78, 0.71, 0.72, and 0.74, respectively, for field soils collected in four regions of South Korea. However, the results showed significant differences in some areas. When the moisture content and electrical conductivity of soil from each region were included in the multiple regression analysis, the cross-validation results showed superior output with no significant difference (p &gt; 0.05). Keywords: Colorimetry, Phosphorus, Precision agriculture, Sensor, Soil.
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Nosov, K. V., Y. G. Bespalov, O. V. Vysotska, H. M. Strashnenko, and E. S. Ivanova. "Modeling the colorimetric parameters of microcosms to improve the operation modes of test microecosystems." Bionics of Intelligence 1, no. 90 (2018): 31–36. https://doi.org/10.30837/bi.2018.1(90).04.
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The article is devoted to the determination of the microcosms system colorimetric parameters, providing an increase in the colorimetric uniformity of the surface, as part of the test micro ecosystems., An idealized system trajectory was obtained with the help of discrete models of dynamic systems. It reflects many non-recurring combinations of colorimetric parameters of the microcosm surface. The efficiency of using the found system colorimetric parameter was verified by image processing of the gastropod pulmonary mollusk, the nature of which is a sign of the presence or absence of toxicity. This is an important factor to increase the effectiveness of the test microecosystems use in real situations — by eliminating the masking effect of the microcosm surface diversity on colorimetric signs of toxicity.
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Yoo, Woo Sik, Jung Gon Kim, Kitaek Kang, and Yeongsik Yoo. "Development of Static and Dynamic Colorimetric Analysis Techniques Using Image Sensors and Novel Image Processing Software for Chemical, Biological and Medical Applications." Technologies 11, no. 1 (2023): 23. http://dx.doi.org/10.3390/technologies11010023.
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Colorimetric sensing techniques for point(s), linear and areal array(s) were developed using image sensors and novel image processing software for chemical, biological and medical applications. Monitoring and recording of colorimetric information on one or more specimens can be carried out by specially designed image processing software. The colorimetric information on real-time monitoring and recorded images or video clips can be analyzed for point(s), line(s) and area(s) of interest for manual and automatic data collection. Ex situ and in situ colorimetric data can be used as signals for process control, process optimization, safety and security alarms, and inputs for machine learning, including artificial intelligence. As an analytical example, video clips of chromatographic experiments using different colored inks on filter papers dipped in water and randomly blinking light-emitting-diode-based decorative lights were used. The colorimetric information on points, lines and areas, with different sizes from the video clips, were extracted and analyzed as a function of time. The video analysis results were both visualized as time-lapse images and RGB (red, green, blue) color/intensity graphs as a function of time. As a demonstration of the developed colorimetric analysis technique, the colorimetric information was expressed as static and time-series combinations of RGB intensity, HSV (hue, saturation and value) and CIE L*a*b* values. Both static and dynamic colorimetric analysis of photographs and/or video files from image sensors were successfully demonstrated using a novel image processing software.