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Dissertations / Theses on the topic 'Column chromatography'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

UMEMURA, TOMONARI, RYO KOMIYAMA, and KAZUHIRO YAMAMOTO. "NUMERICAL SIMULATION ON FLOW IN COLUMN CHROMATOGRAPHY." World Scientific Publishing, 2013. http://hdl.handle.net/2237/20053.

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2

Chow, W. M. L. "Capilliary column gas chromatography in forensic science." Thesis, University of Strathclyde, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371945.

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3

Leow, Pei Ling. "In-column electrochemical detection for liquid chromatography." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/5295.

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This research focuses on the development of whole column detection (WCD) for liquid chromatography (LC). The WCD uses electrochemical techniques for detecting the analytes passing through the separation column. Electrode array for in-column electrochemical detection (ICED) is fabricated along the separation column to enable whole column separation monitoring and allow better understanding on the affinity of particular analyte to the stationary and mobile phases. Numerical models were built to understand the feasibility and differences of electrochemical detection within an unpacked and packed

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4

Malkin, Douglas Scott, and Douglas Scott Malkin. "An Investigation of a Novel Monolithic Chromatography Column, Silica Colloidal Crystal Packed Columns." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/193936.

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Many researchers have investigated ways to improve the separation power of conventional chromatography, most notable is the development of ultra-high performance liquid chromatography (UHPLC). However, only slight improvements in separation efficiency have been achieved up to this point, and unfortunately, modern reversed phase liquid chromatography (RPLC) methods do not have high enough resolving power to analyze complex proteomic mixtures.Uniformly sized silica particles from 10 nm to 1 micron are known to self-assemble into a highly ordered face centered cubic crystal. Silica colloidal crys

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5

Kehoe, Jonathan Joseph. "Total marine lipid profiling by short column gas chromatography /." Internet access available to MUN users only, 2003. http://collections.mun.ca/u?/theses,157942.

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6

Dewoolkar, Veeren. "Continuous Stationary Phase Gradients for Planar and Column Chromatography." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4469.

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Surfaces that exhibit a gradual change in their chemical and/or physical properties are termed as surface gradients. Based on the changes in properties they are classified either as physical or chemical gradients. Chemical gradients show variations in properties like polarity, charge, functionality concentration and have found potential applications in fields of biology, physics, biosensing, catalysis and separation science. In this dissertation, surface gradients have been prepared using controlled rate infusion (CRI). CRI is a simple method in which a surface gradient is formed by carrying o

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7

Riddell, Nicole. "Packed Column Supercritical Fluid Chromatography : Applications in Environmental Chemistry." Doctoral thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-56826.

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Although gas and liquid chromatography have emerged as dominant separation techniques in environmental analytical chemistry, these methods do not allow for the concurrent analysis of chemically diverse groups of persistent organic pollutants (POPs). There are also a small number of compounds which are not easily amenable to either of these traditional separation techniques. The main objective of this thesis was to address these issues by demonstrating the applicability of packed column supercritical fluid chromatography (pSFC) coupled to mass spectrometry (MS) in various aspects of environment

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8

Heng, Kien Chhiev. "Effect of column temperature on elution order in gas chromatography." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-01312009-063555/.

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9

Alexander, Christine. "A study of column switching liquid chromatography in drug analysis." Thesis, Robert Gordon University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308697.

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10

Akbar, Muhammad. "Chip-Scale Gas Chromatography." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/56566.

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Instrument miniaturization is led by the desire to perform rapid diagnosis in remote areas with high throughput and low cost. In addition, miniaturized instruments hold the promise of consuming small sample volumes and are thus less prone to cross-contamination. Gas chromatography (GC) is the leading analytical instrument for the analysis of volatile organic compounds (VOCs). Due to its wide-ranging applications, it has received great attention both from industrial sectors and scientific communities. Recently, numerous research efforts have benefited from the advancements in micro-electromecha

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11

Chienthavorn, Orapin. "Detection in superheated water chromatography." Thesis, Loughborough University, 1999. https://dspace.lboro.ac.uk/2134/32394.

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Superheated water has been used successfully as an eluent in liquid chromatography and has been coupled to various modes of detection, ultraviolet (UV), fluorescence, and nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). A number of compounds were examined on poly(styrene-divinylbenzene) (PS-OVB), polybutadiene (PBO), and octadecylsilyl bonded silica (OOS) column with isothermal and temperature programmes.

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12

Clark-Baker, Karen N. "Investigation of column and instrumental parameters for fast gas chromatography analysis." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-11182008-063322/.

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13

Chan, Ryan. "Density Modulated Semi-Packed Micro Gas Chromatography Columns." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/83448.

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With the continued evolution of MEMS-based gas chromatography, the drive to develop new standalone systems with lower power consumptions and higher portability has increased. However, with improvements come tradeoffs, and trying to reduce the pressure drop requirements of previously reported semi-packed columns causes a significant sacrifice in separation efficiency. This thesis covers the techniques for evaluating the separation column in a gas chromatography system as well as the important parameters that have the most effect on a column’s efficiency. Ionic liquids are introduced as a stable

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14

Hoffman, Brian Jeffrey. "Analysis of Alcohol and Alkylphenol Polyethers via Packed Column Supercritical Fluid Chromatography." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/11174.

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Alkylphenol ethoxylates (APEOs), alcohol ethoxylates (AEOs), and alcohol propoxylates (APOs) are non-ionic surfactants used in daily care products and detergents. They are formed as an oligomeric series with a varying distribution, which determines their commercial application. The goal of the research performed was the development of sample characterization methods for non-ionic surfactants utilizing supercritical fluid chromatography (SFC) under mild instrument operating conditions. The aryl group present in APEOs allowed ultraviolet (UV) detection, with an equal molar response for oligom

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15

Montenegro, Ana M. C. "Studies of column packings for polymer separations by size exclusion and interactive chromatography." Thesis, Loughborough University, 1986. https://dspace.lboro.ac.uk/2134/11754.

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The chromatographic behaviour of a new column packing for high performance size exclusion chromatography based on crosslinked polyacrylamide particles was studied. Experimental retention data for poly(ethylene glycol)and poly(ethylene oxide) standards in water and water-methanol mixtures showed that the separation mechanism is size exclusion, since a universal calibration plot based on hydrodynamic volume was obtained. For polysaccharide standards in water and water-methanol (80/20), the universal calibration was valid for molecular weights above 4 * 10~. Below this value, secondary mechanisms

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16

Ghugare, Tushar. "Exploring Zirconia as a Column Packing Material." TopSCHOLAR®, 2010. http://digitalcommons.wku.edu/theses/194.

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Zirconia is one of the most promising column packing materials for High Performance Liquid Chromatography (HPLC). The perfect HPLC support material should be energetically homogenous, have a high surface area on which different chemical species can reversibly attach and be physically and chemically stable over a wide range of pH, temperature and solvent conditions. Most existing supports do not have all of these properties. This project is also focused on a proteomics study. Zirconia, hafnium oxide and titanium oxide which are some of the more promising materials currently available, can be us

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17

Burdette, Diana Marguerite. "Interfacing packed column supercritical fluid chromatography with mass spectrometry using the particle beam interfaceBurdette." Thesis, Georgia Institute of Technology, 1995. http://hdl.handle.net/1853/27555.

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18

Armstrong, Stephanye Dawn. "The effects of carrier gas viscosity on column efficiency in capillary gas chromatography." Thesis, This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-06162009-063602/.

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19

Wang, Anzi. "Microchip Thermal Gradient Gas Chromatography." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/4300.

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Although the airbath oven is a reliable heating method for gas chromatography (GC), resistive heating is needed for higher analytical throughput and on-site chemical analysis because of size, heating rate and power requirements. In the last thirty years, a variety of resistive heating methods were developed and implemented for both benchtop and portable GC systems. Although fast heating rates and low power consumption have been achieved, losses in column efficiency and resolution, complex construction processes and difficulties experienced in recovering damaged columns have also become problem

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20

Motley, Curtis Bobby. "The evaluation of an argon and helium highly efficient microwave induced plasma as an element selective detector for packed column super critical fluid chromatography." Diss., Virginia Tech, 1990. http://hdl.handle.net/10919/37740.

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21

Illa, Vila Xavier. "A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer." Doctoral thesis, Universitat de Barcelona, 2010. http://hdl.handle.net/10803/1518.

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This thesis describes a novel column format to be used in pressure-driven reversed-phase liquid chromatography. The column contains an ordered pillar array where the stationary phase is supported, and it is fabricated in cyclo olefin polymer (COP) by means of standard replication techniques. The pillar array column format has shown much better efficiencies than the other traditional liquid chromatography column formats due to the order achieved with the controlled pillar packing. A particularity of this work is that the unmodified surface of the pillars acts directly as the stationary phase du

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22

Ng, C. K. S. "Optimal design, operation and control of sequential multi-column chromatography for separation of biomolecules." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1419846/.

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The increasingly stringent regulatory requirements on the quality and cost-effectiveness of biopharmaceuticals as well as the escalating upstream titre are placing considerable pressure on downstream bioprocessing. In particular, batch chromatography is fast becoming the process bottleneck due to its limited capacity and expensive operation. Recent developments in multi-column counter-current chromatography (MCC) for the separation of biomolecules have demonstrated the potential to overcome these limitations. However, the uptake of MCC processes by the biopharmaceutical industry is hindered by

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23

Strode, J. Thompson B. III. "Impact of modified carbon dioxide mobile phases on detection on packed-column supercritical fluid chromatography." Diss., Virginia Tech, 1995. http://hdl.handle.net/10919/38095.

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The advantages of supercritical fluid chromatography (SFC) and supercritical fluid extraction (SFE) which utilize supercritical fluids (SF) as the mobile phase are being realized by the scientific community. SF are more advantageous than traditionally used organic solvents because SF exhibits the solvating strength of liquids while maintaining the higher mass transport properties of gases. Currently, the most common SF which is CO₂ is available in two preparations of pure CO₂ and helium headspace CO₂. SFC of polyaromatic hydrocarbons (PAH) was performed with pure carbon dioxide and helium head

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24

Cripe, M. Kathleen Leslie. "Syntheses and characterization of a t-OCTYLCALIX[5]ARENE derivatized capillary column for gas chromatography." Connect to online version at OhioLINK ETD Connect to online version at Digital.Maag, 1998. http://hdl.handle.net/1989/3749.

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25

Strode, J. Thompson B. "Impact of modified carbon dioxide mobile phases on detection on packed-column supercritical fluid chromatography /." This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-06062008-155212/.

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26

Alsudairy, Ziad. "Identification of Major Organic Constituents of Saffron Isolated by Solid Phase Extraction and Column Chromatography." DigitalCommons@Robert W. Woodruff Library, Atlanta University Center, 2019. http://digitalcommons.auctr.edu/cauetds/200.

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Water extracts of saffron, a spice derived from the plant Crocus Sativus L. obtained from India and Iran, were analyzed by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). The components in the extracts were separated on four different solid phase extraction cartridges with four different solvents and by silica gel column. Analysis was done by GC-MS, LC-MS and UV-Vis spectroscopy. The extracts separated into three distinct bands (two bright yellow and one orange) on the silica gel column. Based on GC-MS, the extracted compounds show many structu

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27

Aggarwal, Pankaj. "High-Performance Polymer Monoliths for Capillary Liquid Chromatography." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/4236.

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This dissertation focuses on improving the chromatographic efficiency of polymeric organic monoliths by characterizing and optimizing the bed morphology. In-situ characterization techniques such as capillary flow porometry (CFP), 3-dimensional scanning electron microscopy (3D SEM) and conductivity measurements were developed and implemented to quantitatively characterize the morphology of poly(ethylene glycol) diacrylate (PEGDA) monoliths. The CFP measurements for monoliths prepared by the same procedure in capillaries with different diameters (i.e., 75, 150, and 250 μm) clearly showed a chang

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28

Onesti, Riccardo. "Characterization of a ceramic monolithic support for affinity protein chromatogrophy." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2017.

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Affinity chromatography is a process for the separation of biomolecules from complex mixtures. Its versatility, high selectivity and efficiency make it the most currently used method in the final stages of purification of pharmaceutical and food industries, where a high degree of purity is required. Conventional affinity chromatography uses packed beads as solid support and suffers of several limitations, such as high material costs, high operating costs, intraparticle diffusion as primary transport phenomenon and difficulties in column packing. Therefore, it is necessary to develop and char

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29

Hamdan, Emad Aerospace Civil &amp Mechanical Engineering Australian Defence Force Academy UNSW. "Extended macroscopic dispersion model with applications to confined packed beds and capillary column inverse gas chromatography." Awarded by:University of New South Wales - Australian Defence Force Academy. School of Aerospace, Civil and Mechanical Engineering, 2008. http://handle.unsw.edu.au/1959.4/38732.

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Until present, many researchers relied on the conventional plug flow dispersion models to analyse the concentration profiles obtained from the tracer injection experiments to evaluate the dispersion coefficients in packed beds. The Fickian concept in the limit of long time duration is assumed to be applicable and it implies that the mean-square displacement of the tracer profile is constant with time and the concentration profile is Gaussian. There were very few studies on identifying the conditions under which this assumption is valid and delineate the range of applicability of the existing p

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30

MacFarlane, John K., and Philip M. Gschwend. "Field analysis of groundwater for volatile organic contaminants using on-column aqueous injection capillary gas chromatography." MIT Energy Lab, 1987. http://hdl.handle.net/1721.1/18198.

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Northeast Utilities Service Company, Southern California Edison Company, Empire State Electric Energy Research Corporation, American Power Service Company, Standard Oil Company and New England Power Service Company under the MIT Energy Laboratory Utilites Program

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31

LOUREIRO, RENAN F. "Caracterização físico-química da foliculotrofina humana (hFSH) recombinante e de suas subunidades, por cromatografia líquida de alta eficiência (HPLC) em fase reversa: comparação com a preparação de referência de hFSH de origem hipofisária do 'National Hormone and Pituitary Program' dos EUA." reponame:Repositório Institucional do IPEN, 2006. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11467.

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Alli, Azaam. "Analysis of organomercurials in environmental and biological samples by capillary column gas chromatography with atomic fluorescence detection." FIU Digital Commons, 1994. http://digitalcommons.fiu.edu/etd/1069.

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The general method for determining organomercurials in environmental and biological samples is gas chromatography with electron capture detection (GC-ECD). However, tedious sample work up protocols and poor chromatographic response show the need for the development of new methods. Here, Atomic Fluorescence-based methods are described, free from these deficiencies. The organomercurials in soil, sediment and tissue samples are first released from the matrices with acidic KBr and cupric ions and extracted into dichloromethane. The initial extracts are subjected to thiosulfate clean up and the org

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McLean, Michael M. "Phenyl Functionalized Sol-gel Silica Sorbent for Capillary Microextraction and Chromia-Based Sol-gel Ucon Stationary Phase for Capillary Gas Chromatography." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5374.

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The first chapter of this thesis presents an introduction to sol-gel methodology whose usefulness as a synthetic route will be demonstrated with two applications in chromatography. The first application involves the fabrication of a capillary micro-extraction (CME) device by coating a phenyl functionalized extracting phase on the inner surface of a fused silica capillary for analyte pre-concentration. The device was coupled on-line to a RP-HPLC system and practicality was demonstrated using allergens as target analytes. The allergens chosen as model analytes are typically found in fragrance pr

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LEBRE, DANIEL T. "Desenvolvimento de metodologia para a determinacao de herbicidas e inseticidas em aguas superficiais utilizando extracao liquido-solido e cromatografia liquida de alta eficiencia." reponame:Repositório Institucional do IPEN, 2000. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10809.

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Rocco, Anna. "Separation of Enantiomers by Means of NanoO-Liquid Chromatography." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2013~D_20130122_144808-59559.

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Nano-liquid chromatography (nano-LC) was selected as analytical tool to develop different methods for chiral separations. Nano-LC offers several advantages over conventional LC, e.g., low sample requirement, short analysis time, easy coupling with mass spectrometer, and use of small amount of reagents, with a consequent low environmental pollution. In case of chiral separations, where expensive chiral stationary phases or chiral mobile phase additives (CMPA) have to be employed, nano-LC results very useful since it allows to perform analysis with small amount of this costly material. Initiall

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Schnell, Andrew Robert. "Use of decision-centric templates in the design of a separation column for a microscale gas chromatography system." Thesis, Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-07102006-081530/.

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Thesis (M. S.)--Mechanical Engineering, Georgia Institute of Technology, 2007.<br>Dr. Martha Gallivan, Committee Member ; Dr. Chris Paredis, Committee Member ; Dr. Janet K. Allen, Committee Member ; Dr. Peter Hesketh, Committee Co-Chair ; Dr. Farrokh Mistree, Committee Co-Chair.

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Schneider, Mark S. "Analysis of Organic Pollutants by Micro Scale Liquid-Liquid Extraction and On-column Large Volume Injection Gas Chromatography." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/46329.

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The analysis of organic pollutants in water is traditionally done following EPA procedures which commonly use liquid-liquid extraction. One liter of water is extracted three times with 60 mL of an organic solvent. The extract is concentrated and analyzed by gas chromatography. This procedure is time consuming and can cause losses of semi-volatile components, in addition to requiring a relatively large amount of organic solvent (180 mL). By performing the extraction directly in a GC autosampler vial using one milliliter of contaminated water and one milliliter of organic solvent, then inject

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Surrao, Alicia M. "Advancing Column Chromatography by Improving Mobile Phase Chemistry for the Separation of Trace Uranium, Plutonium, Strontium, and Barium." University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1529666911529026.

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Duncan, Warwick. "The measurement and modelling of the intracrystalline diffusion of cyclohexane in ZSM-5 using zero length column chromatography." Doctoral thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/8743.

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Bibliography: p. 97-102.<br>Zeolites are important solid acid catalysts largely because of their microporous nature giving them strong shape selective properties. This shape selectivity may be improved by depositing an inert (e. g. silaceous) layer on the surface of the catalyst crystals, which inertises non-shape selective surface acid sites and increases diffusional transport resistances. This thesis presents an investigation, using the zero length column (ZLC) method, into changes in the diffusional properties of cyclohexane in ZSM-5 that has had tetraethoxysilane deposited on the surface i

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Jones, David. "Synthesis, fractionation, characterisation and toxicity of naphthenic acids from complex mixtures." Thesis, University of Plymouth, 2013. http://hdl.handle.net/10026.1/1612.

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Amongst the polar organic compounds occurring in unrefined and refined crude oils and the associated polluted production waters, complex mixtures of acids, known historically as naphthenic acids (NAs), have achieved prominence. This is particularly because NAs have been designated a toxicant class of concern in the oil sands process-affected water (OSPW) that has accumulated in vast quantities following exploitation of the oil sands of Northern Alberta, Canada in recent years. However, though there have been calls for NAs to be added to pollutant inventories, at the initiation of the current s

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OLIVEIRA, JOAO E. de. "Purificacao de hormonio de crescimento humano recombinante obtido no espaco periplasmico de ESCHERICHIA COLI, visando sua aplicacao clinica." reponame:Repositório Institucional do IPEN, 1999. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10755.

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Lefebvre, David. "Étude de nouveaux matériaux poreux pour le développement de micro-colonnes en silicium pour la chromatographie en phase gazeuse." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10314/document.

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En chromatographie en phase gazeuse, la miniaturisation ouvre la voie pour de nouveaux appareils portables, consommant peu d'énergie et de gaz et permettant des analyses directement sur site en évitant ainsi le transport d'échantillons. Depuis les années 70, l'essentiel des travaux s'est focalisé sur les hydrocarbones les plus lourds, avec 5 atomes de carbones ou plus. Ce travail expose le développement de nouvelles phases stationnaires de silice mésostructurées pour la séparation des alcanes légers (avec moins de 5 atomes de carbones) dans des colonnes micro-fabriquées, suivant deux procédés

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Ortega, Scarlet Nere. "Síntese e avaliação de sorventes seletivos MIP e RAMIP para análise online de fluoroquinolonas em matrizes complexas ambientais por column-switching LC-MS/MS." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-15092017-112940/.

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A elevada introdução de antibióticos no ambiente aquático tem aumentado a preocupação em relação ao desenvolvimento de microrganismos resistentes aos agentes antimicrobianos e seu potencial impacto na biota aquática. Com o objetivo de monitorar a presença, persistência e destinação desses fármacos no meio ambiente, há a necessidade de se desenvolver métodos analíticos altamente sensíveis a baixas concentrações desses antibióticos, na ordem de ng L-1

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FURGERI, CAMILO. "Efeito do processamento por radiacao de sup(60)Co na erva-mate (llex paraguariensis)." reponame:Repositório Institucional do IPEN, 2009. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9375.

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Zope, Anjali U. (Anjali Umesh). "Synthesis and Characterization of Methylated PCU Dimers." Thesis, University of North Texas, 1993. https://digital.library.unt.edu/ark:/67531/metadc500801/.

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Conversion of 1-Methylpentacyclo[5.4.0.0²⋅⁶.0³⋅¹⁰.0⁵⋅⁹]undecane- 8,11-dione into the corresponding mono(ethylene ketal) followed by Wolff-Kishner reduction resulted in a mixture of two isomers (i.e., 1- and 7-methyl-8-[2',-(1',3',dioxolano)]pentacyclo[5.4.0.0²⋅⁶.0³⋅¹⁰.0⁵⋅⁹] undecane. Hydrolysis of each isomer in turn resulted in 1- and 7- methyl pentacyclo[5.4.0.0²⋅⁶.0³⋅¹⁰.0⁵⋅⁹ ]undecan-8-ones (i.e.,"methylated PCU-8-ones"), respectively. "Titanium-promoted reductive dimerization of each of the methylated pentacycloundecane (PCU)-8-ones afforded mixtures of "methylated PCU alkene dimers". Indi

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Cáhová, Miroslava. "Stanovení těžkých polycyklických aromatických uhlovodíků v půdách a sedimentech." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216379.

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This diploma thesis will be focused on the identification and quantification of PAHs with molecular mass exceeding 278 Da by separation and spectrometric methods available at the laboratories of ICTEP.

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Balci, Oguz. "Affinity chromatographic purification of recombinant human growth hormone." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/2/12609269/index.pdf.

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The purpose of the study is to purify human growth hormone from the fermentation broth by affinity chromatography. For this purpose, human growth hormone specific oligonucleotide aptamers are selected among an aptamer library<br>selected oligonucleotides were synthesized and used as ligands. Effect of pH on ligand-human growth hormone complex formation was investigated and the highest complex formation was obtained at pH= 7.0. Human growth hormone is separated from the fermentation broth with 99.8% purity and 41% overall yield. The equilibrium data obtained was described by Langmuir type isoth

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KUSSUMI, TEREZA A. "Desenvolvimento de método multirresíduo para determinação de pesticidas benzimidazóis, carbamatos e triazinas em milho por cromatografia líquida acoplada à espectrometria de massas em Tandem e sua certificação." reponame:Repositório Institucional do IPEN, 2007. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11583.

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Made available in DSpace on 2014-10-09T12:53:32Z (GMT). No. of bitstreams: 0<br>Made available in DSpace on 2014-10-09T13:58:56Z (GMT). No. of bitstreams: 0<br>Dissertação (Mestrado)<br>IPEN/D<br>Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP

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Kirk, E. M. "Biomedical applications of narrow-bore liquid chromatography with computer-aided detection : Application of multivariate digital techniques to biomedical samples in narrow-bore column high-performance liquid chromatography with photodiode array detection." Thesis, University of Bradford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384276.

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Patterson, Andrea. "Sorption of Anionic Organic Contaminants to Goethite." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1543427642730863.

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