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Journal articles on the topic 'Column chromatography'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Memon, Najma, Tahira Qureshi, Muhammad Iqbal Bhanger, and Muhammad Imran Malik. "Recent Trends in Fast Liquid Chromatography for Pharmaceutical Analysis." Current Analytical Chemistry 15, no. 4 (2019): 349–72. http://dx.doi.org/10.2174/1573411014666180912125155.

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Background: Liquid chromatography is the workhorse of analytical laboratories of pharmaceutical companies for analysis of bulk drug materials, intermediates, drug products, impurities and degradation products. This efficient technique is impeded by its long and tedious analysis procedures. Continuous efforts of scientists to reduce the analysis time resulted in the development of three different approaches namely, HTLC, chromatography using monolithic columns and UHPLC. Methods: Modern column technology and advances in chromatographic stationary phase including silica-based monolithic columns

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2

Patel, Niti Bharatbhai, Surati Jasmina, Akbari Ashok, Patel Sagar, Shah Ketan, and Solanki Divya. "A Review on UHPLC Instrumentation with Advancements." Journal of Advances in Pharmaceutical Sciences 1, no. 1 (2023): 33–44. https://doi.org/10.5281/zenodo.7932671.

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<em>The Ultra High Performance Liquid Chromatography (UHPLC) is the technique of liquid chromatography which improves speed, resolution and sensitivity. The instrumentation involves the advance requirement that helps the quick response of the peaks. The advances in instrumentation improve the fast separation with the help of UHPLC column involved in the front line of liquid chromatography column developed by giving higher quality chromatographic data in less time. The advancement in column like Columns packed with 2.6-2.7-μm SCPs, Columns packed with sub-2-μm SCPs and Charged surface-par

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3

Firestone, David. "Determination of Dioxins and Furans in Foods and Biological Tissues: Review and Update." Journal of AOAC INTERNATIONAL 74, no. 2 (1991): 375–84. http://dx.doi.org/10.1093/jaoac/74.2.375.

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Abstract Determination of trace residues of polychlorlnated dlbenzo- p-dloxlns and dibenzofurans (PCDDs and PCDFs) In various matrixes Is carried out by a limited number of laboratories In the United States, Canada, and other countries. Current methods for analysis of foods and biological tissues Include a combination of preparation, extraction, cleanup, isolation, determination, and Identity confirmation procedures. Soxhlet, liquid/liquid, solid-phase, and column extraction procedures are used as well as treatment with acid or base before solvent extraction. Cleanup and isolation steps Includ

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4

Patel, Nishiben, Krishna R. Gupta, and Milind J. Umekar. "An Overview of Monolithic Column: Types, Parameters and Applications." Journal of Drug Delivery and Therapeutics 12, no. 4-S (2022): 223–31. http://dx.doi.org/10.22270/jddt.v12i4-s.5521.

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The column is the main component for chromatographic separation. Nowadays, monolithic columns are graining more popularity in the field of separation media for liquid chromatography. The monolith columns possess great potential as compared to the conventional packed column in terms of preparing complex mixtures. These columns provide various properties like higher permeability, high-efficiency fast separations, high flow rate with lower backpressure, fast mass transfer kinetics with a high binding capacity. It is categories into three columns and they are organic monolithic column, inorganic m

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5

Roehrer, Simon, and Mirjana Minceva. "Evaluation of Inter-Apparatus Separation Method Transferability in Countercurrent Chromatography and Centrifugal Partition Chromatography." Separations 6, no. 3 (2019): 36. http://dx.doi.org/10.3390/separations6030036.

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In the countercurrent chromatography and centrifugal partition chromatography, separation method transfer and scale-up is often described as an easy and straightforward procedure. Separation methods are usually developed on lab scale columns and subsequently transferred using linear scale-up factors to semi-preparative or preparative columns of the same column design. However, the separation methods described in the literature have been developed on various columns of different design and size. This is accompanied by differences in the separation behavior of the columns and therefore makes sep

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6

HORTON>, BRENDAN. "Chromatography column." Nature 379, no. 6566 (1996): 659–60. http://dx.doi.org/10.1038/379659a0.

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7

Chakravarti, Bulbul, Buddhadeb Mallik, and Deb N. Chakravarti. "Column Chromatography." Current Protocols Essential Laboratory Techniques 00, no. 1 (2008): 6.2.1–6.2.14. http://dx.doi.org/10.1002/9780470089941.et0602s00.

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8

Sandesh, J. Srikanth Sandesh, Shyamala, Kurva Swapana, Sirra Balaiah, and J. V. C. Sharma. "A REVIEW ON FLASH CHROMATOGRAPHY AND ITS PHARMACEUTICAL APPLICATIONS." Journal of Biomedical and Pharmaceutical Research 10, no. 1 (2021): 120–24. http://dx.doi.org/10.32553/jbpr.v10i1.850.

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Earlier column chromatography was used for preparative purposes as well as for reaction control in organic synthesis. Column chromatography is an extremely tedious stage in any laboratory and can quickly become a point of congestion for any process lab. Flash chromatography is a technique developed as a modification of preparative column chromatography. This is an air pressure driven technique comprising of medium and short column chromatography, optimised for rapid separation of organic compounds. Modern flash chromatographic system consists of pre- packed plastic cartridges where in the solv

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9

Zhang, Jing, and Hongye Zhang. "Research progress on the detection of water-soluble vitamins in food using liquid chromatography." Theoretical and Natural Science 37, no. 1 (2024): 158–69. http://dx.doi.org/10.54254/2753-8818/37/20240183.

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Currently, liquid chromatography is widely used for the analysis and determination of water-soluble vitamins (WSVs) in functional foods. However, due to the diversity of sample characteristics and the requirement for purification accuracy, the chromatographic conditions and specific methods used vary. This paper reviews the chromatographic conditions of liquid chromatography techniques for detecting WSVs in food, as well as the selection of liquid chromatography methods in different application fields. The chromatographic conditions include chromatographic columns, column temperature, mobile p

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10

Peterson, Robert E., Gail M. Shannon, and Odette L. Shotwell. "Purification of Cyclopiazonic Acid by Liquid Chromatography." Journal of AOAC INTERNATIONAL 72, no. 2 (1989): 332–35. http://dx.doi.org/10.1093/jaoac/72.2.332.

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Abstract A purification procedure for cyclopiazonic acid has been developed, using sequential preparative and semi-preparative liquid chromatography. Crude cyclopiazonic acid (324 mg) was extracted from a 1 L fermentation medium with chloroform-methanol (80 + 20), dried, dissolved in chloroform, and chromatographed on an oxalic acid/ silica preparative column with chloroform-methanol (99 + 1) as the eluant. A semi-preparative oxalic acid/silica column and chloroform- methanol (99.5 + 0.5) were then used for rechromatography of the partially purified cyclopiazonic acid. This second chromatograp

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11

Ramesh, Dattatraya Bhusal* Deepali Mahir Nahar Prashant Bhimrao Dalvi. "REVIEW ON: FLASH COLUMN CHROMATOGRAPHY." INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH 07, no. 01 (2017): 7353–58. https://doi.org/10.5281/zenodo.1006789.

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In previous days, Column chromatography was used for qualitative as well as quantitative analysis but Column chromatography is an extremely time consuming process. This leads to the development of novel preparative liquid chromatography in which mobile phase flows down by positive air pressure called as Flash chromatography. Flash Chromatography is a rapid form of preparative column chromatography. Flash chromatography, also known as medium pressure chromatography, was popularized several years ago by Clark Still of Columbia University, as an alternative to slow and often inefficient gravity-f

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12

Jamshaid, Tahir, Khalid Sajjad Feras, Majed Musalim Albluwi, and Abid Hussain. "Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis." Arab Journal of Forensic Sciences and Forensic Medicine 6, Special Issue (2024): 165–74. http://dx.doi.org/10.26735/ikrk2529.

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Reverse Phase High Performance Liquid Chromatography (RP-HPLC) is an efficient and reliable technique employed in pharmaceutical and forensic analysis. The assay determination in pharmaceutical and certain molecular detection is performed using this technique. Performance of chromatographic columns play a central role in development of more efficient separation methods. It is one of the main component of system suitability testing in a chromatographic system. The column efficiency can be assessed by using various traditional methods and reproducible results can be evaluated using liquid chroma

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13

Omelchuk, Sergii T., Alina I. Syrota, and Anna V. Blagaia. "THE NEED FOR IMPROVEMENT OF FUNGICIDES RESIDUAL QUANTITIES CONTROL METHODS IN THE CONDITIONS OF THE DOMESTIC REGULATORY BASE HARMONIZATION." Wiadomości Lekarskie 75, no. 10 (2022): 2455–61. http://dx.doi.org/10.36740/wlek202210126.

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The aim: To develop highly sensitive analytical methods for the determination of the systemic phenylamide class fungicide – Metalaxyl-M residues in watermelons and grapes to reduce the risk of hazardous effects on workers’ and public health. Materials and methods: Conditions for Metalaxyl-M detection by gas-liquid chromatography (GLC) using a chromatographic capillary column SH-Rxi-5ms (length – 30 m, inner diameter – 0.25 mm, layer thickness – 0.25 μm) were determined. The optimal conditions for chromatography of Metalaxyl-M were established: column thermostat temperature – 220°С, evaporator

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14

Hatfield, Robert G., and Andrew D. Turner. "Rapid Liquid Chromatography for Paralytic Shellfish Toxin Analysis Using Superficially Porous Chromatography with AOAC Official MethodSM 2005.06." Journal of AOAC INTERNATIONAL 95, no. 4 (2012): 1089–96. http://dx.doi.org/10.5740/jaoacint.12-005.

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Abstract The bioaccumulation of paralytic shellfish toxins in mussels, oysters, cockles, hard clams, razors, and king scallops is monitored in England, Scotland, and Wales by AOAC Official MethodSM 2005.06 LC-with fluorescence detection (FLD). One of the commonly perceived disadvantages of using this method is the long turnaround time and low throughput in a busy laboratory environment. The chromatographic analysis of each sample typically utilizes a 15 min cycle time to achieve toxin oxidation product separation and column equilibration prior to subsequent analysis. A standard RP C18 analytic

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15

Leela, Bhaskar K. 1* Vasubabu P. 2. Kanthi Sheela A. 3. Sri Pavan Kumar Ch 4. Sunand Kumar M. 5. "A REVIEW ON CORE SHELL TECHNOLOGY IN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY: DRUG ANALYSIS." Journal of Pharma Research 7, no. 10 (2018): 231–35. https://doi.org/10.5281/zenodo.1471873.

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<strong><em>ABSTRACT</em></strong> <strong><em>C</em></strong><em>olumn is the heart of the chromatographic systems. Now a days in order to increase the separation efficiency a new technology of column packed with core shell particles was used in HPLC .The core shell particles revolutionized the chromatography industry by providing faster methods without sacrificing resolution .this specialized particles provides higher efficiency and through put which leads to a reduction in solvent consumption. The literature here by describe the performance difference between  the core shell particles

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16

Efimenko, A. P., I. I. Naumenko, V. K. Soboleva, and M. N. Baldin. "Application of multicapillary columns for the fast separation of BTEX aromatic hydrocarbons from ambient air samples." Аналитика и контроль 29, no. 1 (2025): 75–82. https://doi.org/10.15826/analitika.2025.29.1.005.

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Air pollution affects public health and the environment, and must be controlled. Measurement of the spatial distribution of pollutants by a mobile laboratory requires fast response of the analytical instruments used. Optical methods of detecting nitrogen oxides, suspended particles, ozone, sulfur dioxide and carbon monoxide in the air allow performing up to 10 measurements per second. Determination of the content of aromatic hydrocarbons (benzene, toluene, ethylbenzene and xylenes, collectively designated as BTEX) is carried out by mass spectrometry and gas chromatography. However, a mass spec

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17

Nguyen, Thinh Duc, Thu Thi Nguyet Nguyen, Diem Ngoc Duong, Son Pham Ngoc Chu, and Thuoc Linh Tran. "Preparation of an immunoaffinity chromatography column for chloramphenicol binding." Science and Technology Development Journal 19, no. 4 (2016): 14–23. http://dx.doi.org/10.32508/stdj.v19i4.688.

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Anti-chloramphenicol rabbit antibody was covalently bound to Sepharose CL 4B-CNBr gel at 5, 10 and 15 mg antibody per mL gel and was fed into columns to prepare three respective immunoaffinity chromatography columns for chloramphenicol (CAP) binding. Examination of CAP-binding efficiency of these columns being fed with 0.5, 1 and 10 ng CAP showed that the column with 10 mg antibody per ml gel (IAC-CAP-10 column) could bind more than 90 % of the CAP amount having been fed into the column at all three examined CAP amounts. This IAC-CAP-10 column showed a coefficient of variants (CV) between colu

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18

Arzu Ibragimova, Arzu Ibragimova. "BENEFITS OF USING A FID TO MEASURE THE MULTICOMPONENT GAS MIXTURES." PIRETC-Proceeding of The International Research Education & Training Centre 27, no. 06 (2023): 131–39. http://dx.doi.org/10.36962/piretc27062023-131.

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The development of the oil and gas complex is one of the priority areas of the Azerbaijan economy. Oil and gas are among the most competitive Azerbaijan goods and are in high and stable demand from global consumers. Therefore, increased attention is paid to product quality. One of the methods for quality control of petroleum products is gas chromatography. Today it is a widely used physical and chemical research method. The capabilities of a gas chromatography are mainly determined by the enormous separating power of the chromatographic columns and the characteristics of the detectors. If the

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19

Sentkowska, Aleksandra, Magdalena Biesaga, and Krystyna Pyrzynska. "Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids inGenista tinctoriaExtract." Journal of Analytical Methods in Chemistry 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/3789348.

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Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of organic modifier were studied. On ZIC column retention factors were observed to be inversely proportional to the buffer content in the mobile phase, which is the typical partitioning mechanism. In the case

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20

Barth, Howard G., William E. Barber, Charles H. Lochmueller, Ronald E. Majors, and F. E. Regnier. "Column liquid chromatography." Analytical Chemistry 60, no. 12 (1988): 387–435. http://dx.doi.org/10.1021/ac00163a025.

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21

Barth, Howard G., Charles H. Lochmuller, Ronald E. Majors, and Fred E. Regnier. "Column liquid chromatography." Analytical Chemistry 58, no. 5 (1986): 211–50. http://dx.doi.org/10.1021/ac00296a016.

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22

Oden, Katarina, and Jaap de Zeeuw. "Column Overload in PLOT Columns." LCGC North America, 2022. http://dx.doi.org/10.56530/lcgc.na.uw7875j1.

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Injecting too much sample onto a chromatographic system will eventually overload the column. Analytical chemists are concerned about column overloading because it reduces column efficiency, and, as a result, the resolution is sacrificed. Surprisingly, there is little data in the literature about the overloading of the porous layer open tubular (PLOT) columns. This month’s article looks at the basic types of column overload, volume and mass, and defines the variables that most affect the sample loading capacity in PLOT column chromatography.

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23

"A Concise Review on High-Performance Liquid Chromatography." Journal of Pharmaceutical Research 8, no. 2 (2023). http://dx.doi.org/10.33140/jpr.08.02.13.

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A review of High-Performance Liquid Chromatography included an introduction, chromatographic terms, different classes, and types of HPLC techniques. Also included a brief introduction to HPLC principle and instrumentation. Columns, pumps, and detectors used in HPLC are also included in detail with their efficiency. Applications of the HPLC and overall new advantage cues are included in this review article.: High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples. The chromat

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24

Sajeevan J, Siddharth Jaya, Reza Salehi, Ryan Jacob Burk, et al. "Chiral Chromatographic Separation of Fifteen New Hexahydroquinoline Derivatives." Chirality 37, no. 5 (2025). https://doi.org/10.1002/chir.70033.

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ABSTRACTHexahydroquinoline (HHQ) scaffold attracts great interest due to its diverse pharmacological activities. HHQ framework also includes 1,4‐dihydropyridine (DHP) ring, the pharmacophore of the most popular group of drugs known as calcium channel blockers, which are frequently used in the treatment of cardiovascular conditions. In this work, we synthesized 15 HHQ‐based potential calcium channel modulators (EM1–EM15) as racemic mixtures. The 15 chiral compounds were assayed on five high‐efficiency liquid chromatography columns containing different small chiral selectors: derivatized β‐cyclo

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25

"COLUMN CHROMATOGRAPHY." A-to-Z Guide to Thermodynamics, Heat and Mass Transfer, and Fluids Engineering c (2006). http://dx.doi.org/10.1615/atoz.c.colchr.

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26

Chakravarti, Bulbul, Buddhadeb Mallik, and Deb N. Chakravarti. "Column Chromatography." Current Protocols Essential Laboratory Techniques 13, no. 1 (2016). http://dx.doi.org/10.1002/cpet.6.

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27

Meyers, C. L. F. "Column Chromatography." Current Protocols in Nucleic Acid Chemistry 3, no. 1 (2000). http://dx.doi.org/10.1002/0471142700.nca03es03.

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28

Franco, Jimmy. "Column Chromatography." Journal of Visualized Experiments, February 5, 2024. http://dx.doi.org/10.3791/10217.

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29

Ramesh, Dattatraya Bhusal* Deepali Mahir Nahar Prashant Bhimrao Dalvi. "REVIEW ON: FLASH COLUMN CHROMATOGRAPHY." January 31, 2017. https://doi.org/10.5281/zenodo.2381151.

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In previous days, Column chromatography was used for qualitative as well as quantitative analysis but Column chromatography is an extremely time consuming process. This leads to the development of novel preparative liquid chromatography in which mobile phase flows down by positive air pressure called as Flash chromatography. Flash Chromatography is a rapid form of preparative column chromatography. Flash chromatography, also known as medium pressure chromatography, was popularized several years ago by Clark Still of Columbia University, as an alternative to slow and often inefficient gravity-f

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30

Hemmer, Selina, Sascha K. Manier, Lea Wagmann, and Markus R. Meyer. "Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma." Metabolomics 20, no. 3 (2024). http://dx.doi.org/10.1007/s11306-024-02115-0.

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Abstract Introduction Untargeted metabolomics studies are expected to cover a wide range of compound classes with high chemical diversity and complexity. Thus, optimizing (pre-)analytical parameters such as the analytical liquid chromatography (LC) column is crucial and the selection of the column depends primarily on the study purpose. Objectives The current investigation aimed to compare six different analytical columns. First, by comparing the chromatographic resolution of selected compounds. Second, on the outcome of an untargeted toxicometabolomics study using pooled human liver microsome

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31

"Liquid column chromatography." Journal of Chromatography A 861, no. 2 (1999): 511–37. http://dx.doi.org/10.1016/s0021-9673(00)86516-4.

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32

"Liquid column chromatography." Journal of Chromatography A 784, no. 1 (1997): B243—B294. http://dx.doi.org/10.1016/s0021-9673(00)89099-8.

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"Liquid column chromatography." Journal of Chromatography A 783, no. 1 (1997): B1—B52. http://dx.doi.org/10.1016/s0021-9673(00)89104-9.

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"Liquid column chromatography." Journal of Chromatography A 748, no. 1 (1996): B235—B293. http://dx.doi.org/10.1016/s0021-9673(00)89108-6.

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"Liquid column chromatography." Journal of Chromatography A 747, no. 2 (1996): B115—B173. http://dx.doi.org/10.1016/s0021-9673(00)89112-8.

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"Liquid column chromatography." Journal of Chromatography A 335 (January 1985): B1—B173. http://dx.doi.org/10.1016/s0021-9673(00)91527-9.

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"Liquid column chromatography." Journal of Chromatography A 335 (January 1985): B175—B280. http://dx.doi.org/10.1016/s0021-9673(00)91528-0.

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"Liquid column chromatography." Journal of Chromatography A 335 (January 1985): B281—B396. http://dx.doi.org/10.1016/s0021-9673(00)91529-2.

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"Liquid column chromatography." Journal of Chromatography A 335 (January 1985): B397—B514. http://dx.doi.org/10.1016/s0021-9673(00)91530-9.

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"Liquid column chromatography." Journal of Chromatography A 335 (January 1985): B515—B648. http://dx.doi.org/10.1016/s0021-9673(00)91531-0.

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"Liquid column chromatography." Journal of Chromatography A 335 (January 1985): B649—B840. http://dx.doi.org/10.1016/s0021-9673(00)91532-2.

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"Chromatography column simulator." Journal of Chemical Education 70, no. 11 (1993): 882. http://dx.doi.org/10.1021/ed070p882.

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"Liquid column chromatography." Journal of Chromatography A 714, no. 1 (1995): B253—B307. http://dx.doi.org/10.1016/s0021-9673(00)80001-1.

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"Liquid column chromatography." Journal of Chromatography A 714, no. 2 (1995): B383—B429. http://dx.doi.org/10.1016/s0021-9673(00)80005-9.

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"Liquid column chromatography." Journal of Chromatography A 714, no. 2 (1995): B489—B500. http://dx.doi.org/10.1016/s0021-9673(00)80010-2.

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"Liquid column chromatography." Journal of Chromatography A 713, no. 2 (1995): B135—B187. http://dx.doi.org/10.1016/s0021-9673(00)80015-1.

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"Liquid column chromatography." Journal of Chromatography A 713, no. 1 (1995): B1—B53. http://dx.doi.org/10.1016/s0021-9673(00)80024-2.

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"Liquid column chromatography." Journal of Chromatography A 747, no. 1 (1996): 1–43. http://dx.doi.org/10.1016/s0021-9673(00)80029-1.

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"Liquid column chromatography." Journal of Chromatography A 748, no. 2 (1996): B363—B409. http://dx.doi.org/10.1016/s0021-9673(00)80033-3.

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"Liquid column chromatography." Journal of Chromatography A 748, no. 2 (1996): B473—B483. http://dx.doi.org/10.1016/s0021-9673(00)80038-2.

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