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Journal articles on the topic 'Combined medium for autologous'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Naumann, A., N. Rotter, J. Bujía, and J. Aigner. "Tissue Engineering of Autologous Cartilage Transplants for Rhinology." American Journal of Rhinology 12, no. 1 (1998): 59–64. http://dx.doi.org/10.2500/105065898782102972.

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In reconstructive surgery there is increasing demand for cartilage transplants to fill defects, especially nose and/or outer ear defects. Tissue engineering is one of the most modern pathways to generate autologous cartilage transplants. Isolated chondrocytes obtained from a tiny patient's biopsy were seeded on bioresorbable preshaped cell carriers to provide a 3-dimensional cell arrangement as in vivo. The combined use of these cell carriers in form of a non-woven mesh and a constant medium perfusion was performed to generate a cartilage-like cell-polymer-construct, which was finally subcutan

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2

Muir, Andrew J. T., Andrew J. Niehaus, Joseph W. Lozier, et al. "Autologous platelet-rich plasma effects on Staphylococcus aureus–induced chondrocyte death in an in vitro bovine septic arthritis model." American Journal of Veterinary Research 83, no. 2 (2022): 119–26. http://dx.doi.org/10.2460/ajvr.21.01.0007.

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Abstract OBJECTIVE To investigate the chondroprotective effects of autologous platelet-rich plasma (PRP), ampicillin-sulbactam (AmpS), or PRP combined with AmpS (PRP+AmpS) in an in vitro chondrocyte explant model of bovine Staphylococcus aureus–induced septic arthritis. SAMPLE Autologous PRP and cartilage explants obtained from 6 healthy, adult, nonlactating Jersey-crossbred cows. ProcedureS Autologous PRP was prepared prior to euthanasia using an optimized double centrifugation protocol. Cartilage explants collected from grossly normal stifle joints were incubated in synovial fluid (SF) alone

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Li, Chengke, Weihai Song, Yanwen Lei, Songgen Peng, Weiying Chu, and Guochao Deng. "Clinical Application of Artificial Dermis and Autologous Skin in Repairing Skin and Soft Tissue Defects of Hands and Feet with Bone Exposure Injuries." Evidence-Based Complementary and Alternative Medicine 2021 (October 16, 2021): 1–8. http://dx.doi.org/10.1155/2021/1202826.

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Patients with skin and soft tissue defects are very common. Mild trauma often causes mild skin damage, while severe injuries are often accompanied by bone and tendon exposure, which brings great pain to patients. For the defect of skin and soft tissue, the traditional treatment methods are mostly medium or full-thickness skin or skin flap transplantation. These methods are effective in wound repair, but there are still many problems. In recent years, with the improvement of tissue engineering technology, the use of artificial skin to repair various skin wounds is gradually becoming clinical, a

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Jiang, Aimei, Ming Li, Wenjing Duan, Yilong Dong, and Yanmei Wang. "Improvement of the Survival of Human Autologous Fat Transplantation by Adipose-Derived Stem-Cells-Assisted Lipotransfer Combined with bFGF." Scientific World Journal 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/968057.

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Adipose-derived stem cells (ASCs) transplanted along with autologous adipose tissue may improve fat graft survival; however, the efficacy of ASCs has been diluted by low vascularization. This study was designed to test the hypothesis that basic fibroblast growth factor (bFGF) may improve the effects of ASCs because it owns the property to boost angiogenesis. In the present study, human fat tissues were mixed with ASCs, ASCs plus 100 U bFGF, or medium as the control and then injected subcutaneously into immunologically compromised nude mice for 12 weeks. Our findings demonstrated that mixture w

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Roeth, Alexander, Lisa Schneider, Gabriela Baerlocher, and Ulrich Duehrsen. "A Better Strategy To Extend the Proliferative Lifespan of Human T-Cells In Vitro." Blood 106, no. 11 (2005): 2398. http://dx.doi.org/10.1182/blood.v106.11.2398.2398.

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Abstract Telomerase maintains telomere length by adding TTAGGG repeats to the 3′-ends of chromosomes. In human T-cells telomerase is transiently expressed upon activation and stimulation and, as shown previously, telomerase levels are able to control their lifespan. To understand the effects of culture parameters on telomerase activity and cell lifespan is of critical importance to improve T-cell expansion. Here, we investigated the influence of culture conditions and of stimulation on lifespan, clonogenicity (number of positive wells), cell cycle and telomerase activity in T-cells in vitro. I

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Pham, Phuc Van, Ngoc Bich Vu, Thuy Thi-Thanh Dao, Ha Thi-Ngan Le, Lan Thi Phi, and Ngoc Kim Phan. "ID: 1016 Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages." Biomedical Research and Therapy 4, S (2017): 91. http://dx.doi.org/10.15419/bmrat.v4is.294.

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Endothelial progenitor cells (EPCs) play an important role in angiogenesis. However, they exist in limited numbers in the human body. This study was aimed to produce EPCs, for autologous transplantation, using direct reprogramming of skin fibroblasts under GMP-compliant conditions. Fibroblasts were collected and cultured from the skin in DMEM/F12 medium supplemented with 5% activated platelet-rich plasma and 1% antibiotic-antimycotic solution. They were then transfected with mRNA ETV2 and incubated in culture medium under hypoxia (5% oxygen) for 14 d. Phenotype analysis of transfected cells co

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S, Gopinathan. "Clinical Study about Latissimus Dorsi Based Breast Reconstruction at a Tertiary Health Centre in Mangalore, India." Journal of Evolution of Medical and Dental Sciences 10, no. 34 (2021): 2905–9. http://dx.doi.org/10.14260/jemds/2021/592.

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BACKGROUND The most common cancer among urban Indian women is breast cancer. Mastectomy causes loss of body image and negatively impacts the quality of life. Restoration of body image is an important step for breast cancer survivors. Recent reconstruction options are autologous or implant-based reconstruction or combined approach. This present clinical study was done to describe about latissimus dorsi based breast reconstruction (BR) at a tertiary health centre. METHODS This retrospective study was conducted at a tertiary health care centre from Jun 2018 to Dec 2020. Eligible desiring patients

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Vranckx, Jan Jeroen, and Margot Den Hondt. "Tissue engineering and surgery: from translational studies to human trials." Innovative Surgical Sciences 2, no. 4 (2017): 189–202. http://dx.doi.org/10.1515/iss-2017-0011.

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AbstractTissue engineering was introduced as an innovative and promising field in the mid-1980s. The capacity of cells to migrate and proliferate in growth-inducing medium induced great expectancies on generating custom-shaped bioconstructs for tissue regeneration. Tissue engineering represents a unique multidisciplinary translational forum where the principles of biomaterial engineering, the molecular biology of cells and genes, and the clinical sciences of reconstruction would interact intensively through the combined efforts of scientists, engineers, and clinicians. The anticipated possibil

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Qin, Shuai, Shuai Zheng, Bing Qi, Rui Guo, and Guanghui Hou. "Decellularized Human Stromal Lenticules Combine with Corneal Epithelial-Like Cells: A New Resource for Corneal Tissue Engineering." Stem Cells International 2019 (December 7, 2019): 1–10. http://dx.doi.org/10.1155/2019/4252514.

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The lack of donor corneal tissue or the immunological rejection remains a challenge for individuals with limbal stem cell deficiency (LSCD) who are treated with keratoplasty. Numerous lenticules which were extracted by small incision lenticule extraction (SMILE) appear to be useful materials for keratoplasty. In order to reduce the incidence of allograft rejection, lenticules would be decellularized. Lenticules which were treated with liquid nitrogen and nucleases had no cellular and nuclear materials remained. Human induced pluripotent stem cells (iPSCs) can be generated from the patient who

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Lemoli, RM, C. Gasparetto, DA Scheinberg, MA Moore, BD Clarkson, and SC Gulati. "Autologous bone marrow transplantation in acute myelogenous leukemia: in vitro treatment with myeloid-specific monoclonal antibodies and drugs in combination." Blood 77, no. 8 (1991): 1829–36. http://dx.doi.org/10.1182/blood.v77.8.1829.1829.

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Abstract We report the results of a preclinical study comparing four different purging protocols using a promyelocytic human cell line HL-60 and myeloid leukemic progenitor cells (colony-forming unit-leukemic [CFU- L]) from acute myelogenous leukemia (AML) patients assayed in semisolid culture. We studied the antileukemic effect of (1) Single-cycle complement-mediated lysis by two different monoclonal antibodies (MoAbs) (M195 [CD33] and F23 [CD13] 40 micrograms/mL), reactive with distinct antigens found on early myeloid cells and monocytes, used alone and in combinations; (2) 4-Hydroperoxycycl

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Lemoli, RM, C. Gasparetto, DA Scheinberg, MA Moore, BD Clarkson, and SC Gulati. "Autologous bone marrow transplantation in acute myelogenous leukemia: in vitro treatment with myeloid-specific monoclonal antibodies and drugs in combination." Blood 77, no. 8 (1991): 1829–36. http://dx.doi.org/10.1182/blood.v77.8.1829.bloodjournal7781829.

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We report the results of a preclinical study comparing four different purging protocols using a promyelocytic human cell line HL-60 and myeloid leukemic progenitor cells (colony-forming unit-leukemic [CFU- L]) from acute myelogenous leukemia (AML) patients assayed in semisolid culture. We studied the antileukemic effect of (1) Single-cycle complement-mediated lysis by two different monoclonal antibodies (MoAbs) (M195 [CD33] and F23 [CD13] 40 micrograms/mL), reactive with distinct antigens found on early myeloid cells and monocytes, used alone and in combinations; (2) 4-Hydroperoxycyclophospham

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Świerczek-Lasek, Barbara, Lukasz Tolak, Lukasz Bijoch, et al. "Comparison of Muscle Regeneration after BMSC-Conditioned Medium, Syngeneic, or Allogeneic BMSC Injection." Cells 11, no. 18 (2022): 2843. http://dx.doi.org/10.3390/cells11182843.

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For many years optimal treatment for dysfunctional skeletal muscle characterized, for example, by impaired or limited regeneration, has been searched. Among the crucial factors enabling its development is finding the appropriate source of cells, which could participate in tissue reconstruction or serve as an immunomodulating agent (limiting immune response as well as fibrosis, that is, connective tissue formation), after transplantation to regenerating muscles. MSCs, including those derived from bone marrow, are considered for such applications in terms of their immunomodulatory properties, as

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13

Anders, Sven, Martin Volz, Hubert Frick, and Jörg Gellissen. "A Randomized, Controlled Trial Comparing Autologous Matrix-Induced Chondrogenesis (AMIC®) to Microfracture: Analysis of 1- and 2-Year Follow-Up Data of 2 Centers." Open Orthopaedics Journal 7, no. 1 (2013): 133–43. http://dx.doi.org/10.2174/1874325001307010133.

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Microfracture (MFx) is currently the recommended option for the treatment of small cartilage defects but is not regarded as suitable for the treatment of defects larger than 2.5 cm2. To extent its applicability to medium-sized defects MFx has been combined with a collagen type I/III matrix (Chondro-Gide®). This technique is called Autologous Matrix-Induced Chondrogenesis (AMIC®) and meanwhile a clinically established treatment option for localized full-thickness small- to medium-sized cartilage defects. Despite its more spreading clinical use, clinical data published so far are limited to main

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Smagur, Andrzej, Iwona Mitrus, Sebastian Giebel, et al. "Comparision Of Different Cryoprotective Solution - Albumin Vs Autologous Plasma - Its Effect On Cell Recovery and Clonogenic Potential Of Cryopreserved Peripheral Blood Hematopoietic Stem and Progenitor Cells." Blood 122, no. 21 (2013): 4522. http://dx.doi.org/10.1182/blood.v122.21.4522.4522.

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Background Peripheral blood is a preferable source of hematopoietic stem and progenitor cells (HSPCs) used for autologous transplantation. HSPCs are mobilized to peripheral blood and collected by leukapheresis. Prior to cryopreservation the cells need to be processed including the addition of cryoprotective mixture as dimethyl sulfoxide (DMSO) prediluted in human serum albumin solution (HSAS). In Europe there is no commercially available albumin manufactured in packs with tubing which would enable the use of sterile tubing welder. Alternatively cryoprotective solution can be prepared using aut

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Wilgenhof, S., A. M. Van Nuffel, D. Benteyn, et al. "Therapeutic vaccination with an autologous TriMix-Dendritic cell vaccine combined with sequential interferon alfa-2b in patients with advanced melanoma." Journal of Clinical Oncology 27, no. 15_suppl (2009): 9024. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.9024.

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9024 Background: Electroporation of dendritic cells (DC) with mRNA encoding fusion-proteins of a HLA-class II targeting signal and a melanoma associated antigen (MAA) together with mRNA encoding CD40 ligand, a constitutively active TLR4 and CD70 (TriMix) improves the immunostimulatory capacity of autologous DC. Methods: Following leukapheresis, immature DCs (derived from adherent PBMC cultured for 6 days in IL-4 / GM-CSF supplemented medium) are electroporated with mRNA encoding MAGE-A3, MAGE-C2, Tyrosinase and gp100 linked to DC-LAMP, and TriMix mRNA. TriMix-DC (12.5 106/antigen) are cryopres

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Jiao, Weijie, Chen Liu, Jingxin Shan, Zhiyuan Kong, and Xiaohong Wang. "Construction and Evaluation of Small-Diameter Bioartificial Arteries Based on a Combined-Mold Technology." Polymers 14, no. 15 (2022): 3089. http://dx.doi.org/10.3390/polym14153089.

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Arterial stenosis or blockage is the leading cause of cardiovascular disease, and the common solution is to substitute the arteries by autologous veins or bypass the blood vessels physically. With the development of science and technology, arteries with diameter larger than 6 mm can be substituted by unbiodegradable polymers, such as polytetrafluoroethylene, clinically. Nevertheless, the construction of a small-diameter (less than 6 mm) artery with living cells has always been a thorny problem. In this study, a suit of combined mold was designed and forged for constructing small-diameter arter

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Kazantsev, A. N., K. P. Chernykh, R. Yu Leader, et al. "Glomus-saving carotid endarterectomy by A. N. Kazantsev. Hospital and medium-remote results." Patologiya krovoobrashcheniya i kardiokhirurgiya 24, no. 3 (2020): 70. http://dx.doi.org/10.21688/1681-3472-2020-3-70-79.

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<p><strong>Aim.</strong> Analysis of the results of hospital and medium-long-term results obtained using a new method of glomus-saving carotid endarterectomy (CEE) according to A. N. Kazantsev.</p><p><strong>Methods.</strong> This prospective study was conducted during January 2018 to April 2020 on 214 patients who were operated for occlusive stenotic lesions of the internal carotid artery (ICA) using holomus-saving CEE as per the method described by <br />A.N. Kazantsev. The average observation duration was 17.2 ± 6.5 months.<br />Glomus-s

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Bodine, DM, T. Moritz, RE Donahue, et al. "Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34+ bone marrow cells." Blood 82, no. 7 (1993): 1975–80. http://dx.doi.org/10.1182/blood.v82.7.1975.1975.

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Abstract Retroviral mediated gene transfer into stem cells has been proposed as therapy for many inherited hematopoietic diseases. Deficiency of the enzyme adenosine deaminase (ADA) results in depletion of T lymphocytes, causing severe combined immunodeficiency syndrome (SCIDS). In this report, we describe retroviral mediated gene transfer of a murine ADA cDNA into Rhesus monkey hematopoietic stem cells. Immunoselected CD34+ bone marrow cells were exposed to medium containing the ADA retrovirus during culture on a stromal cell line engineered to express the transmembrane form of stem cell fact

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Bodine, DM, T. Moritz, RE Donahue, et al. "Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34+ bone marrow cells." Blood 82, no. 7 (1993): 1975–80. http://dx.doi.org/10.1182/blood.v82.7.1975.bloodjournal8271975.

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Retroviral mediated gene transfer into stem cells has been proposed as therapy for many inherited hematopoietic diseases. Deficiency of the enzyme adenosine deaminase (ADA) results in depletion of T lymphocytes, causing severe combined immunodeficiency syndrome (SCIDS). In this report, we describe retroviral mediated gene transfer of a murine ADA cDNA into Rhesus monkey hematopoietic stem cells. Immunoselected CD34+ bone marrow cells were exposed to medium containing the ADA retrovirus during culture on a stromal cell line engineered to express the transmembrane form of stem cell factor. After

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Baba, Rikiya, Tomohiro Onodera, Masatake Matsuoka, et al. "Bone Marrow Stimulation Technique Augmented by an Ultrapurified Alginate Gel Enhances Cartilage Repair in a Canine Model." American Journal of Sports Medicine 46, no. 8 (2018): 1970–79. http://dx.doi.org/10.1177/0363546518770436.

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Background: The optimal treatment for a medium- or large-sized cartilage lesion is still controversial. Since an ultrapurified alginate (UPAL) gel enhances cartilage repair in animal models, this material is expected to improve the efficacy of the current treatment strategies for cartilage lesions. Hypothesis: The bone marrow stimulation technique (BMST) augmented by UPAL gel can induce hyaline-like cartilage repair. Study Design: Controlled laboratory study. Methods: Two cylindrical osteochondral defects were created in the patellar groove of 27 beagle dogs. A total of 108 defects were divide

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Li, Xin, Wei Ding, and Jun Wang. "Negative pressure sealing drainage with antibiotic bone cement for the treatment of skin and soft tissue defects in the older population with bone exposure." Journal of Wound Care 32, no. 2 (2023): 104–8. http://dx.doi.org/10.12968/jowc.2023.32.2.104.

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Objective: To observe the clinical efficacy of vacuum sealing drainage (VSD) combined with antibiotic bone cement in the treatment of skin and soft tissue defects of the extremities with bone exposure in the older population. Method: From January 2016 to December 2018, VSD combined with antibiotic bone cement was used to treat 12 older patients with skin and soft tissue defects of the extremities and bone exposure. The study cohort consisted of eight male patients and four female patients aged between 60–95 years, with a median of 75 years. The injury sites included four cases of hand, one cas

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Poletti, Valentina, Sabine Charrier, Samia Martin, et al. "Preclinical Development of Gene Therapy for X-Linked Severe Combined Immunodeficiency (SCID-X1)." Blood 128, no. 22 (2016): 4705. http://dx.doi.org/10.1182/blood.v128.22.4705.4705.

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Abstract X-linked severe combined immunodeficiency (SCID-X1) is caused by mutations in the gene encoding the interleukin-2 receptor γ chain (IL2RG), and is characterized by lack of response to common γ-chain-dependent cytokines and profound defects in T-, B- and NK-cell functions. Previous gene therapy clinical trials based on autologous transplantation of hematopoietic stem/progenitor cells (HSPCs) genetically corrected with MLV-derived retroviral vectors expressing IL2RG in non-conditioned patients showed long-lasting restoration of T-cell immunity in most cases but resulted in vector-induce

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Yoshida, Atsushi, Reiko Tanaka, Akira Kodama, Naoki Yamamoto, Aftab A. Ansari, and Yuetsu Tanaka. "Identification of HIV-1 Epitopes that Induce the Synthesis of a R5 HIV-1 Suppression Factor by Human CD4+T Cells Isolated from HIV-1 Immunized Hu-PBL SCID Mice." Clinical and Developmental Immunology 12, no. 4 (2005): 235–42. http://dx.doi.org/10.1080/17402520500391557.

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We have previously reported that immunization of the severe combined immunodeficiency (SCID) mice reconstituted with human peripheral blood mononuclear cells (PBMC) (hu-PBL-SCID mice) with inactivated human immunodeficiency virus type-1 (HIV-1)-pulsed-autologous dendritic cells (HIV-DC) elicits HIV-1-reactive CD4+T cells that produce an as yet to be defined novel soluble factorin vitrowith anti-viral properties against CCR5 tropic (R5) HIV-1 infection. These findings led us to perform studies designed to identify the lineage of the cell that synthesizes such a factorin vitroand define the epit

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Castaño, C., C. Cubells, S. Remollo, MR García-Sort, and M. Terceño. "Use of a complete autologous blood recovery system (the Sorin Xtra® Autotransfusion System) during mechanical thrombectomy of extensive cerebral venous sinus thrombosis." Interventional Neuroradiology 23, no. 5 (2017): 531–37. http://dx.doi.org/10.1177/1591019917720908.

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Background The endovascular therapy for cerebral venous sinus thrombosis (CVST) is currently accepted as a second-line treatment for patients who have failed or those in whom systemic anticoagulation is contraindicated or in a subgroup of patients presenting with rapid neurologic deterioration. A number of different mechanical and pharmacologic endovascular strategies have been reported, either as separate or combined approaches. These new catheters and aspiration systems have a high power and vacuum capacity, which carries a risk of anemization of the patient and hypovolemic shock, being nece

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Bąkowski, Paweł, Kamilla Grzywacz, Agnieszka Prusińska, Kinga Ciemniewska-Gorzela, Justus Gille, and Tomasz Piontek. "Autologous Matrix-Induced Chondrogenesis (AMIC) for Focal Chondral Lesions of the Knee: A 2-Year Follow-Up of Clinical, Proprioceptive, and Isokinetic Evaluation." Journal of Functional Biomaterials 13, no. 4 (2022): 277. http://dx.doi.org/10.3390/jfb13040277.

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(1) Background: The autologous matrix-induced chondrogenesis (AMIC) is a bio-orthopedic treatment for articular cartilage damage. It combines microfracture surgery with the application of a collagen membrane. The aim of the present study was to report a medium-term follow-up of patients treated with AMIC for focal chondral lesions. (2) Methods: Fourty-eight patients treated surgically and 21 control participants were enrolled in the study. To evaluate the functional outcomes, the proprioceptive (postural stability, postural priority) and isokinetic (peak value of maximum knee extensor and flex

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Ossendorff, Robert, Sibylle Grad, Martin J. Stoddart та ін. "Autologous Chondrocyte Implantation in Osteoarthritic Surroundings: TNFα and Its Inhibition by Adalimumab in a Knee-Specific Bioreactor". American Journal of Sports Medicine 46, № 2 (2017): 431–40. http://dx.doi.org/10.1177/0363546517737497.

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Background: Autologous chondrocyte implantation (ACI) fails in up to 20% of cases. Advanced intra-articular degeneration paired with an inflammatory environment may be closely related to implantation failure. Certain cytokines have been identified to play a major role during early osteoarthritis. Purpose: To investigate the effects of tumor necrosis factor α (TNFα) and its potential inhibition by adalimumab on cartilage regeneration in an in vitro model of ACI. Study Design: Controlled laboratory study. Methods: Bovine articular chondrocytes were cultivated and transferred at passage 3 to fibr

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Frühwirth, Margareta, Daniela Thaler, Andreas Reinisch, et al. "Combined Action of Endothelial and Mesenchymal Niche Cells to Amplify Hematopoietic Progenitor Expansion in a Humanized System." Blood 112, no. 11 (2008): 2410. http://dx.doi.org/10.1182/blood.v112.11.2410.2410.

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Abstract The hematopoietic stem cell (HSC) niche is an anatomically confined space governing HSC proliferation, differentiation and self renewal. Recent research identified distinct compartments described as stromal and vascular niches. This study was initiated to directly compare mesenchymal stromal cell (MSC) and vascular endothelial progenitor cell (EPC) contribution to niche functions in a humanized co-culture system. MSCs and EPCs were propagated under animal protein-free conditions following recently established protocols. Autologous MSC-EPC pairs were established to avoid donor variatio

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Chai, K., Yq Ai, and Lw Jiang. "Phase II study of dendritic cell vaccination combined with recombinant adenovirus-p53 in treatment for patients with advanced pancreatic carcinoma." Journal of Clinical Oncology 31, no. 15_suppl (2013): 3049. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.3049.

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3049 Background: There are few choices of treatments for advanced pancreatic carcinoma (PC) due to the resistances to chemo- or radio-therapy. Immunotherapy based on dendritic cell (DC) vaccines and p53-based gene therapy are two promising therapeutic modalities. They also demonstrated favorable safety profiles. In this study, we compared the immunological and clinical response between DC vaccine therapy and DC vaccine combined with recombinant adenovirus-p53 (rAd-p53) gene therapy. Methods: Thirty-six patients with a stage IV pancreatic cancer, 21 men and 15 women with an average age of 56.2

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Murlistyarini, Sinta, Lulus Putri Aninda, Ufida Aini Afridafaz, Sri Widyarti, Agustina Tri Endharti, and Teguh Wahju Sardjono. "The combination of ADSCs and 10% PRP increases Rb protein expression on senescent human dermal fibroblasts." F1000Research 10 (June 30, 2021): 516. http://dx.doi.org/10.12688/f1000research.53170.1.

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Background: The senescence process in human dermal fibroblasts (HDFs) is caused by cell cycle withdrawal processes, one of which is the result of the retinoblastoma (Rb) protein being in a hypo-phosphorylated state. Since adipose-derived stem cells (ADSCs) have a paracrine effect, ADSCs were utilized to improve the senescence process of HDFs. The use of non-autologous cell culture media to grow ADSCs can be legally problematic; therefore, platelet-rich plasma (PRP) can be considered as an alternative medium. PRP contains various growth factors that can be used to process the reversal of senesc

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Warth, Ryan J., Polina Matre, Adam Kozemchak, et al. "The Subacromial Bursa is a Viable Source of Autologous Mesenchymal Stem Cells for Rotator Cuff Repair." Orthopaedic Journal of Sports Medicine 7, no. 7_suppl5 (2019): 2325967119S0027. http://dx.doi.org/10.1177/2325967119s00279.

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Objectives: Chronic rotator cuff tears still represent a significant source of morbidity and functional decline in the general population. The purpose of this study was to establish protocols for isolation and expansion of bursa-derived mesenchymal stem cells (BDSCs) and to evaluate their differentiation capacity, including tenogenesis. We hypothesized that BDSCs would be capable of multilineage differentiation (including tenogenesis) and represent an important source for autologous stem cells for patients undergoing rotator cuff repair. Methods: After IRB approval, 10 patients (ages 43-65 yea

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Wang, Feng-Jie, Dan Cui, and Wei-Dong Qian. "Therapeutic Effect of CD4+CD25+ Regulatory T Cells Amplified In Vitro on Experimental Autoimmune Neuritis in Rats." Cellular Physiology and Biochemistry 47, no. 1 (2018): 390–402. http://dx.doi.org/10.1159/000489919.

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Background/Aims: This study aimed to explore whether the adoptive transfusion of autologous CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) has a therapeutic effect on Experimental autoimmune neuritis (EAN) model rats, and it provides new experimental and theoretical bases for the immunotherapy of Guillain-Barre syndrome (GBS). Methods: CD4+CD25+ Tregs were sorted from the spleens of rats using immunomagnetic bead separation techniques combined with flow cytometry. Their in vitro inhibitory function was determined using a lymphocyte proliferation inhibition test, and their purity was confirmed

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Lamana, María L., Rosa M. Yañez, and Juan A. Bueren. "Retroviral Gene Transfer of Suicide Genes in Human T Lymphocytes: Improved Transduction Conditions for a Clinical Protocol." Blood 104, no. 11 (2004): 5247. http://dx.doi.org/10.1182/blood.v104.11.5247.5247.

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Abstract Aiming to control GVHD by means of the transfer of suicide genes into donor T cells, we seeked to optimise the transduction of human T cells using immobilized anti-CD3i/28i MoAbs for T cell stimulation. In previous studies we showed that reducing the concentration of anti-CD3i, from 1,000ng/ml down to 1ng/ml, helps to preserve the cytotoxicity of the samples to an allogeneic stimulus and maintains the susceptibility of the T cells to the retroviral transduction. Aiming to reduce the cost of the manipulation procedure, we also tried to reduce the amount of anti-CD28 MoAb during the act

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Smagur, Andrzej, Iwona Mitrus, Sebastian Giebel, et al. "Effect of Various Concentrations of Dimethyl Sulfoxide (DMSO) on Cell Recovery and Clonogenic Potential of Cryopreserved Peripheral Blood Hematopoietic Progenitors." Blood 118, no. 21 (2011): 1930. http://dx.doi.org/10.1182/blood.v118.21.1930.1930.

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Abstract Abstract 1930 BACKGROUND: Hematopoietic stem cells mobilized to peripheral blood and collected by leukapheresis are predominantly used for autologous transplantation. Prior to cryopreservation the cells need to be processed including the addition of DMSO (dimethyl sulfoxide), which enhance cell survival, but is potentially toxic to stem cell recipient. The most commonly used concentration of DMSO is 10%. The goal of our study was to test if the concentration may be reduced without negative impact on cell recovery and clonogenicity. METHODS: Samples were prospectively collected from 12

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Argiti, Katerina, Mukesch J. Shah, Kevin Joseph, et al. "Platelet rich fibrin and commercial sealants for dural closure in neurosurgery: An in vitro study." PLOS ONE 20, no. 4 (2025): e0319349. https://doi.org/10.1371/journal.pone.0319349.

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Background Watertight closure of the dura mater is essential after neurosurgical interventions to avoid complications such meningitis, intracranial hypotension and surgical site infections. In addition to conventional suturing techniques, various biomaterials have been proposed as sealants to minimize the risk of leakage. We previously reported that patient derived platelet-rich fibrin (PRF), an autologous biomaterial, significantly improves watertight dural closure when applied as an onlay over a standard running suture. To demonstrate the efficacy of this method, we aimed to compare this ort

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Fostier, Karel, Jurgen Corthals, Carlo Heirman, et al. "Immunomodulatory Drugs Restore Effector Cell Immune Functions In Myeloma Patients With Low Disease Burden After Autologous Stem Cell Transplantation." Blood 122, no. 21 (2013): 3214. http://dx.doi.org/10.1182/blood.v122.21.3214.3214.

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Abstract The micro-environment in multiple myeloma (MM) is highly immunosuppressive with increased numbers of regulatory T cells (Tregs) and myeloid derived suppressor cells (MDSCs) favoring tumorcell survival and hampering immunotherapeutic strategies such as dendritic cell vaccination. Immunomodulatory drugs (IMiDs) are known to enhance T- and NK-cell function. In this study we evaluated the effects of low dose (0.5 microM) lenalidomide (Len) and pomalidomide (Pom) on the functionality of CD8+ and CD4+ T cells, MDSCs, Tregs and ex-vivo generated mononuclear derived dendritic cells (moDCs) ob

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Yeung, H. Y., J. Z. Liu, Ling Qin, et al. "A Potent Osteogenic Composite of Stem Cells – Tricalcium Phosphate Ceramic Enhanced the Posterior Spinal Fusion." Key Engineering Materials 288-289 (June 2005): 39–42. http://dx.doi.org/10.4028/www.scientific.net/kem.288-289.39.

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Bone marrow mesenchymal stem cells (BMSCs) possess a high replicative capacity and have the capacity to differentiate into various connective tissue cell types. With the advance in cell culture technique, the BMSCs have been induced to differentiate to osteoblastics linage. To improve the situation of non-union in posterior spinal fusion (PSF), tissue engineering approach to combine BMSCs supported by the calcium phosphate ceramics was applied in PSF and its effect was investigated in the present study. Autologous BMSCs from 16-week-old rabbit tibiae were expanded and induced to differentiate

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Feinman, Rena, Tracy Andrews, Iriana Colorado, et al. "Influence of the Gut Microbiome on Clinical Outcomes in the CPIT-001 Trial, a Phase Ib-IIA Study of Combined Checkpoint Inhibition with Nivolumab and Ipilimumab after Autologous Hematopoietic Stem Cell Transplantation in Patients at High-Risk for Post-Transplant Recurrence." Blood 132, Supplement 1 (2018): 3443. http://dx.doi.org/10.1182/blood-2018-99-117669.

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Abstract Background: Autologous stem cell transplantation (ASCT) remains a standard of care for patients with high-risk and recurrent diffuse large B cell lymphoma (DCLBL), T cell lymphoma (TCL) and multiple myeloma (MM). The discovery that differences in the composition of the gut microbiome contribute to the heterogeneity of response to chemotherapies or immunotherapies has prompted us to investigate the influence of the gut microbiome on progression-free survival (PFS) and overall survival in these patient populations treated with combined nivolumab (Nivo) and ipilimumab (Ipi) in the post-A

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Garcia-Urkia, Nerea, Jon Luzuriaga, Veronica Uribe-Etxebarria, et al. "Enhanced Adipogenic Differentiation of Human Dental Pulp Stem Cells in Enzymatically Decellularized Adipose Tissue Solid Foams." Biology 11, no. 8 (2022): 1099. http://dx.doi.org/10.3390/biology11081099.

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Engineered 3D human adipose tissue models and the development of physiological human 3D in vitro models to test new therapeutic compounds and advance in the study of pathophysiological mechanisms of disease is still technically challenging and expensive. To reduce costs and develop new technologies to study human adipogenesis and stem cell differentiation in a controlled in vitro system, here we report the design, characterization, and validation of extracellular matrix (ECM)-based materials of decellularized human adipose tissue (hDAT) or bovine collagen-I (bCOL-I) for 3D adipogenic stem cell

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Aparicio-Soto, Marina, Caterina Curato, Franziska Riedel, Hermann-Josef Thierse, Andreas Luch, and Katherina Siewert. "In Vitro Monitoring of Human T Cell Responses to Skin Sensitizing Chemicals—A Systematic Review." Cells 11, no. 1 (2021): 83. http://dx.doi.org/10.3390/cells11010083.

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Background: Chemical allergies are T cell-mediated diseases that often manifest in the skin as allergic contact dermatitis (ACD). To prevent ACD on a public health scale and avoid elicitation reactions at the individual patient level, predictive and diagnostic tests, respectively, are indispensable. Currently, there is no validated in vitro T cell assay available. The main bottlenecks concern the inefficient generation of T cell epitopes and the detection of rare antigen-specific T cells. Methods: Here, we systematically review original experimental research papers describing T cell activation

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Kreutz, Marina, Karin Fischer, Petra Hoffmann, et al. "Inhibitory Effects of Lactic Acid on Human Antigen-Specific CD8+ T-Cells." Blood 104, no. 11 (2004): 3844. http://dx.doi.org/10.1182/blood.v104.11.3844.3844.

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Abstract A characteristic feature of inflammatory lesions or tumor sites is local acidosis, which is attributed to the local increase in lactic acid production. We studied the effect of such an acidic environment on the immune functions of antigen-specific CD8+ T-cells by incubating the cells in the presence of various concentrations of lactic acid for up to 48h. CD8+ T-cells were isolated from healthy donors and expanded by weekly stimulation with autologous dendritic cells pulsed with a mutated HLA-A2-binding Melan-A (ELAGIGILTV) peptide. The obtained T cell population consisted of at least

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Golay, Josée, Anna D’amico, Gianmaria Borleri, et al. "Massive, Clinical Grade Expansion Of Polyclonal T Cells Using Blinatumomab For Adoptive Autologous Cellular Therapy Of CLL Patients." Blood 122, no. 21 (2013): 3272. http://dx.doi.org/10.1182/blood.v122.21.3272.3272.

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Abstract Background The combined use of chemotherapy and monoclonal antibodies has proved highly effective for the treatment of CLL but often results in severe life threatening immunosuppression. The development of adoptive therapy with autologous T cells could be clinically relevant to overcome these problems. Methods We have devised a novel, simple and efficient method for ex vivo expansion of normal autologous T cells from the peripheral blood of CLL patients for adoptive therapy, using blinatumomab (CD3xCD19) and rhIL-2 in serum-free medium. The complete phenotype of in vitro expanded T ce

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Merling, Randall K., Colin L. Sweeney, Uimook Choi, et al. "A Platform Minigene AAVS1 Targeted Safe Harbor Approach For Genetic Correction Of iPSC Derived From Patients With Each Of The 5 Genetic Forms Of Chronic Granulomatous Disease." Blood 122, no. 21 (2013): 1024. http://dx.doi.org/10.1182/blood.v122.21.1024.1024.

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Abstract There are 5 genetic forms of chronic granulomatous disease (CGD), resulting from mutations in one of the CYBB, CYBA, NCF1, NCF2, or NCF4 genes encoding, respectively, the subunits of the phagocyte oxidase (phox) gp91phox, p22phox, p47phox, p67phox and p40phox. Phox is required for blood neutrophils and monocytes to produce microbicidal superoxide and hydrogen peroxide. We previously demonstrated zinc finger nuclease (ZFN) targeting of a corrective minigene encoding gp91phox to the AAVS1 safe harbor site of iPSC from a patient with X-linked CGD (XCGD), which restored phox activity in n

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Saitoh, Anri, Miwako Narita, Akie Yamahira, et al. "Effectiveness of WT1 Peptide Vaccination Combined with Imatinib in a Patient with CML in Chronic Phase." Blood 114, no. 22 (2009): 4253. http://dx.doi.org/10.1182/blood.v114.22.4253.4253.

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Abstract Abstract 4253 Cytotoxic T lymphocytes (CTLs) are presumed to kill the relevant antigen-expressing leukemia cells including leukemic stem cells, which display intrinsic resistance against tyrosine kinase inhibitors such as imatinib in CML patients. In order to clarify the safety and effectiveness of WT1 peptide vaccination for the patients with CML, we started WT1 peptide vaccination in combination with imatinib therapy for a CML patient who could not acquire a major molecular response by the administration of imatinib. In addition, we tried to evaluate the kinetics of WT1-specific CTL

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Detterich, Jon, Adam M. Bush, Roberta Miyeko Kato, et al. "Abnormal Red Cell Deformability and Aggregation in Sickle Cell Trait." Blood 120, no. 21 (2012): 1001. http://dx.doi.org/10.1182/blood.v120.21.1001.1001.

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Abstract Abstract 1001 Introduction: SCT occurs in 8% of African Americans and is not commonly associated with clinical disease. Nonetheless, the United States Armed Forces has reported that SCT conveys a 30-fold risk of sudden cardiac arrest and a 200-fold risk from exertional rhabdomyolysis. In fact, rhabdomyolysis in athletes with SCT has been the principal cause of death in NCAA football players in the last decade, leading to recently mandated SCT testing in all Division-1 players. In SCT, RBC sickle only under extreme conditions and with slow kinetics. Therefore, rhabdomyolysis most likel

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Liu, Yu, Li Wang, Haocheng Yu, Samir S. Parekh, Eric Schadt, and Jun Zhu. "ISS stage and network risk score to predict benefits of multiple myeloma treatment options." Journal of Clinical Oncology 38, no. 15_suppl (2020): e20511-e20511. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.e20511.

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e20511 Background: Multiple myeloma (MM) is molecularly heterogeneous with many treatment regimens developed targeting different aspects of the disease. Multi-omics studies are now widely available, facilitating a precision medicine approach for personalized MM treatments. However, data from these studies vary with respect to treatment regimens, patient age, disease stage, and genomic alterations, thus complicating identification of treatment effects. Methods: From a Multiple Myeloma Molecular Causal Network (M3CN) model we previously described, we identified a prognostic subnetwork (prognNET)

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Pascutti, Maria F., Jacqueline M. Tromp, Margot Jak, et al. "Role of T Cell-Derived IL-21 in the Proliferation of Chronic Lymphocytic Leukemia Cells,." Blood 118, no. 21 (2011): 3871. http://dx.doi.org/10.1182/blood.v118.21.3871.3871.

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Abstract Abstract 3871 Introduction: Lymph nodes (LN) from chronic lymphocytic leukaemia (CLL) patients contain characteristic proliferation centres, which are interspersed with CD154+ CD4+ T cells. We have previously shown that in vitro stimulation of peripheral blood (PB) CLL cells with CD154-expressing fibroblasts (3T40L) results in an apoptotic profile similar to the one found in LN (Smit et al, Blood 2007 Feb 15;109(4):1660; Kater et al, Br J Haematol 2004;127(4):404). However this stimulus fails to induce proliferation of CLL cells. In fact, the signals involved in CLL proliferation in v

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Zhao, Bing, Tingjun Dai, Dandan Zhao, et al. "Clinicopathologic Profiles of Sporadic Late-Onset Nemaline Myopathy." Neurology - Neuroimmunology Neuroinflammation 9, no. 4 (2022): e1184. http://dx.doi.org/10.1212/nxi.0000000000001184.

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Background and ObjectivesSporadic late-onset nemaline myopathy (SLONM) is a treatable or otherwise fatal myopathy. Diagnosis of SLONM is still challenging, and no therapeutic consensus has been achieved. Here, we reported the clinicopathologic features and long-term follow-up data of SLONM in a Chinese cohort.MethodsWe performed a retrospective evaluation of clinical, pathologic, and treatment outcomes of 17 patients with SLONM diagnosed between March 1986 and April 2021 at our neuromuscular center. Immunohistochemistry (IHC) with antibodies against 5 Z-disc–associated proteins was performed i

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Ganesan, Saravanan, Ezhilarasi Chendamarai, Jayandharan G. Rao, et al. "Rationale and Efficacy of Bortezomib in the Treatment of Acute Promyelocytic Leukemia in Combination with Arsenic Trioxide: In-Vitro and Phase I Data." Blood 118, no. 21 (2011): 947. http://dx.doi.org/10.1182/blood.v118.21.947.947.

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Abstract Abstract 947 About 5–10% newly diagnosed and about 20–30% of relapsed acute promyelocytic leukaemia (APL) patients will have disease recurrence after receiving currently accepted standards of care. There are a limited number of drugs in the armamentarium for treatment of APL. Preliminary work from our laboratory suggests that stromal cell adhesion mediated drug resistance (CAM-DR) is probably significant with arsenic trioxide (ATO) and is seen both in APL cell lines (n=8; figure 1A) and primary APL cells (n=26; data not shown). Preliminary data suggests that bortezomib (Bo) has cytoto

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Gupta, Rohun, Jithin John, Monik Gupta, et al. "A Cross-Sectional Analysis of Breast Reconstruction with Fat Grafting Content on TikTok." Archives of Plastic Surgery 49, no. 05 (2022): 614–16. http://dx.doi.org/10.1055/s-0042-1756296.

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AbstractAs of November 2021, TikTok has one billion monthly active users and is recognized as the most engaging social media platform. TikTok has seen a surge in users and content creators, ranging from athletes to medical professionals. In the past year, content creators have utilized the app to advocate for social reforms, education, and other uses that were not previously considered. Breast cancer is the most commonly diagnosed cancer in women, with an expected 281,550 new cases of invasive breast cancer in 2021. As more individuals with breast cancer choose to undergo resection, the demand

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Gaundar, Shiva, Emily Blyth, Leighton Clancy, Renee Simms, and David Gottlieb. "In Vitro Generation of Influenza-Virus Specific T Cells for Adoptive Immunotherapy,." Blood 118, no. 21 (2011): 4040. http://dx.doi.org/10.1182/blood.v118.21.4040.4040.

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Abstract Abstract 4040 Influenza viruses cause fatal respiratory infections in stem cell transplant patients. Specific T cells provide long-lived adaptive immunity to influenza and the potential for generating such cells for clinical use was investigated. The inactivated influenza vaccine (Fluvax, CSL Australia) was used as the antigen source. Only reagents and culture media approved for clinical manufacture were used. Monocyte-derived dendritic cells (MoDC) pulsed with Fluvax were used to stimulate autologous PBMC at a responder to stimulator ratio of 10:1. On Day 7, a second stimulation was

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