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Journal articles on the topic 'Complete Cell Biosensor'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Weingart, Oliver G., and Martin J. Loessner. "Nerve cell-mimicking liposomes as biosensor for botulinum neurotoxin complete physiological activity." Toxicology and Applied Pharmacology 313 (December 2016): 16–23. http://dx.doi.org/10.1016/j.taap.2016.10.010.

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Wang, Zhenzhen, Jiawen Cheng, Guimin Dai, Xiaoqi Sun, Xueli Yin, and Yuanyuan Zhang. "The Establishment of a Tobramycin-Responsive Whole-Cell Micro-Biosensor Based on an Artificial Ribozyme Switch." Life 13, no. 7 (2023): 1553. http://dx.doi.org/10.3390/life13071553.

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In this study, a tobramycin concentration-dependent whole-cell micro-biosensor (tob-HHAz) was constructed by fusing a tobramycin aptamer with a hammerhead ribozyme (HHR) from Schistosoma mansoni. The biosensor was obtained by integrating all the modules into one complete RNA sequence, which was easily introduced into E. coli without suffering from harsh external environments. Three independent tobramycin-sensitive RNA structures were identified via high-throughput screening in vivo and were further verified in vitro to undergo the desired self-cleavage reaction. The computation prediction of t

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Tam, Christina C., Yangyang Wang, Wen-Xian Du, Andrew R. Flannery, and Xiaohua He. "Development of a Rapid and Sensitive CANARY Biosensor Assay for the Detection of Shiga Toxin 2 from Escherichia coli." Toxins 16, no. 3 (2024): 148. http://dx.doi.org/10.3390/toxins16030148.

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Shiga-toxin-producing Escherichia coli (STEC) causes a wide spectrum of diseases including hemorrhagic colitis and hemolytic uremic syndrome (HUS). The current Food Safety Inspection Service (FSIS) testing methods for STEC use the Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) protocol, which includes enrichment, cell plating, and genomic sequencing and takes time to complete, thus delaying diagnosis and treatment. We wanted to develop a rapid, sensitive, and potentially portable assay that can identify STEC by detecting Shiga toxin (Stx) using the CANARY (Cellular

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Zaidan, Liena, Inna Novodchuk, Alexander H.Xu, et al. "Rapid, Selective, and Ultra-Sensitive Field Effect Transistor-Based Detection of Escherichia coli." Materials 17, no. 15 (2024): 3648. http://dx.doi.org/10.3390/ma17153648.

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Escherichia coli (E. coli) was among the first organisms to have its complete genome published (Genome Sequence of E. coli 1997 Science). It is used as a model system in microbiology research. E. coli can cause life-threatening illnesses, particularly in children and the elderly. Possible contamination by the bacteria also results in product recalls, which, alongside the potential danger posed to individuals, can have significant financial consequences. We report the detection of live Escherichia coli (E. coli) in liquid samples using a biosensor based on a field-effect transistor (FET) biosen

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Kostryukova, Lyubov V., Anastasia S. Serdyukova, Veronica V. Pronina, Victoria V. Shumyantseva, and Yulia A. Tereshkina. "An Electrochemical Biosensor Analysis of the Interaction of a Two-Vector Phospholipid Composition of Doxorubicin with dsDNA and Breast Cancer Cell Models In Vitro." Pharmaceutics 16, no. 11 (2024): 1412. http://dx.doi.org/10.3390/pharmaceutics16111412.

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Objectives: The main aim of our experiments was to demonstrate the suitability of cell-based biosensors for searching for new anticancer medicinal preparations. Methods: The effect of the substance doxorubicin, doxorubicin embedded in phospholipid nanoparticles, and doxorubicin with phospholipid nanoparticles modified by targeting vectors (cRGD and folic acid) on dsDNA and breast cancer cell lines (MCF-7, MDA-MB-231) was studied. Results: In the obtained doxorubicin nanoforms, the particle size was less than 60 nm. Our study of the percentage of doxorubicin inclusion showed the almost complete

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Dr., E. Gajendran, J. Vignesh Dr., and S. R. Boselin Prabhu Dr. "BIOSENSING WITH U-SHAPED FIBER SMEARED WITH GOLD NANOPARTICLES." International Journal of Multidisciplinary Research and Modern Education 3, no. 1 (2017): 410–13. https://doi.org/10.5281/zenodo.801199.

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Biosensors are logical devices composed of a recognition component of biological origin and a physico-chemical transducer. Immobilization plays a foremost character in developing the biosensor by incorporating both the above mentioned mechanisms. In this paper, an analytical review of fiber optic sensors and biosensors towards real world applications for environmental and clinical monitoring have been reviewed. The establishment of sensor systems has elated recompenses such as measurement in flammable and explosive atmospheres, resistance to electrical noises, trimness, geometrical suppleness,

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Rathi, A. Priya, and A. Vimala Juliet. "A Low Temperature Co-Fired Ceramic Microfluidic Cell Counter." Applied Mechanics and Materials 592-594 (July 2014): 2261–66. http://dx.doi.org/10.4028/www.scientific.net/amm.592-594.2261.

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A three-dimensional microfluidic biosensor has been successfully designed using a low temperature co-fired ceramic (LTCC) technology. This microfluidic sensor consists of mixing, focusing and measuring region. The mixing region is a rectangular shaped channel, to enable the complete mixing of sample and buffered saline solution. An electrode pair in the focusing region uses negative dielectrophoretic forces to direct the cells from all directions of the channel towards the center. The measuring region consists of eleven pairs of gold plated electrodes to measure the change in impedance wheneve

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Uspenskaya, Elena V., Ekaterina Kuzmina, Hoang Thi Ngoc Quynh, Maria A. Komkova, Ilaha V. Kazimova, and Aleksey A. Timofeev. "Influence of Mechanical Loading on the Process of Tribochemical Action on Physicochemical and Biopharmaceutical Properties of Substances, Using Lacosamide as an Example: From Micronisation to Mechanical Activation." Pharmaceutics 16, no. 6 (2024): 798. http://dx.doi.org/10.3390/pharmaceutics16060798.

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Many physical and chemical properties of solids, such as strength, plasticity, dispersibility, solubility and dissolution are determined by defects in the crystal structure. The aim of this work is to study in situ dynamic, dispersion, chemical, biological and surface properties of lacosamide powder after a complete cycle of mechanical loading by laser scattering, electron microscopy, FR-IR and biopharmaceutical approaches. The SLS method demonstrated the spontaneous tendency toward surface-energy reduction due to aggregation during micronisation. DLS analysis showed conformational changes of

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Mavrikou, Sofia, Vasileios Tsekouras, Kyriaki Hatziagapiou, et al. "Angiotensin-Converting Enzyme 2 (ACE2) As a Novel Biorecognition Element in A Cell-Based Biosensor for the Ultra-Rapid, Ultra-Sensitive Detection of the SARS-CoV-2 S1 Spike Protein Antigen." Chemosensors 9, no. 12 (2021): 341. http://dx.doi.org/10.3390/chemosensors9120341.

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Antigen screening for the SARS-CoV-2 S1 spike protein is among the most promising tools for the mass monitoring of asymptomatic carriers of the virus, especially in limited resource environments. Herewith, we report on the possible use of the angiotensin-converting enzyme 2 (ACE2), the natural receptor and entry point of the virus, as a biorecognition element for the detection of the S1 antigen combined with an established bioelectric biosensor based on membrane-engineered cells. The working principle of our approach is based on the measurable change of the electric potential of membrane-engin

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Elinder, Malin, Helena Nordström, Matthis Geitmann, et al. "Screening for NNRTIs with Slow Dissociation and High Affinity for a Panel of HIV-1 RT Variants." Journal of Biomolecular Screening 14, no. 4 (2009): 395–403. http://dx.doi.org/10.1177/1087057109333977.

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A lead optimization library consisting of 800 HIV-1 nonnucleoside reverse transcriptase inhibitors (NNRTIs) was screened in parallel against 4 clinically relevant variants of HIV-1 RT (Wt, L100I, Y181C, and K103N) using a surface plasmon resonance—based biosensor. The aim was to identify inhibitors suitable in specific topical microbicides efficient for preventing the transmission of a range of clinically significant strains of HIV-1. The authors hypothesized that such compounds should have high affinity and slow dissociation rates for multiple variants of the target. To efficiently analyze th

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Kume, Kohei, Liting Chen, Jaewoong Lee, and Markus Müschen. "Autonomous Ca2+ Oscillations Reflect Oncogenic Signaling in B-ALL Cells." Blood 134, Supplement_1 (2019): 1253. http://dx.doi.org/10.1182/blood-2019-130708.

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Background: Engagement of the B-cell receptor (BCR) results in Ca2+ flux and is linked to B-cell survival based on store-operated Ca2+ entry (SOCE), which is triggered by ORAI1 and stromal interaction molecule-1 (STIM1). While normal B-cells release Ca2+ only in response to external stimuli (e.g. BCR-engagement), we recently observed that transformed B-cells exhibit autonomous oscillatory Ca2+ signals that are linked to oncogene activity. Studying a novel dual biosensor system to concurrently measure Ca2+ fluctuations and oncogenic kinase activity in the same B-ALL cells, we discovered that on

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Mukundan, Arvind, Shih-Wei Feng, Yu-Hsin Weng, et al. "Optical and Material Characteristics of MoS2/Cu2O Sensor for Detection of Lung Cancer Cell Types in Hydroplegia." International Journal of Molecular Sciences 23, no. 9 (2022): 4745. http://dx.doi.org/10.3390/ijms23094745.

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In this study, n-type MoS2 monolayer flakes are grown through chemical vapor deposition (CVD), and a p-type Cu2O thin film is grown via electrochemical deposition. The crystal structure of the grown MoS2 flakes is analyzed through transmission electron microscopy. The monolayer structure of the MoS2 flakes is verified with Raman spectroscopy, multiphoton excitation microscopy, atomic force microscopy, and photoluminescence (PL) measurements. After the preliminary processing of the grown MoS2 flakes, the sample is then transferred onto a Cu2O thin film to complete a p-n heterogeneous structure.

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Mukundan, Arvind, Shih-Wei Feng, Yu-Hsin Weng, et al. "Optical and Material Characteristics of MoS2/Cu2O Sensor for Detection of Lung Cancer Cell Types in Hydroplegia." International Journal of Molecular Sciences 23, no. 9 (2022): 4745. http://dx.doi.org/10.3390/ijms23094745.

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In this study, n-type MoS2 monolayer flakes are grown through chemical vapor deposition (CVD), and a p-type Cu2O thin film is grown via electrochemical deposition. The crystal structure of the grown MoS2 flakes is analyzed through transmission electron microscopy. The monolayer structure of the MoS2 flakes is verified with Raman spectroscopy, multiphoton excitation microscopy, atomic force microscopy, and photoluminescence (PL) measurements. After the preliminary processing of the grown MoS2 flakes, the sample is then transferred onto a Cu2O thin film to complete a p-n heterogeneous structure.

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Kuzevanova, A. Yu, O. A. Khalmurzaev, A. A. Borunova, et al. "Study of the action of peripheral blood T-lymphocytes on renal cell carcinoma cells in model systems." Cancer Urology 18, no. 4 (2023): 15–24. http://dx.doi.org/10.17650/1726-9776-2022-18-4-15-24.

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Background. The introduction of immunotherapy based on immune checkpoint inhibitors has significantly improve the effectiveness of kidney cancer treatment. Nevertheless, not all patients respond to such treatment and there are no reliable predictive markers. Therefore, the development of a model system for assessing the cellular immune response to a tumor seems to be an urgent task.Aim. Development of a model to assess the T-cell immune response was the focus of this study.Materials and methods. Primary tumor cell culture and peripheral blood T-cell fraction were obtained under standard steril

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BISWAS, Subhasis B., Stephen FLOWERS, and Esther E. BISWAS-FISS. "Quantitative analysis of nucleotide modulation of DNA binding by DnaC protein of Escherichia coli." Biochemical Journal 379, no. 3 (2004): 553–62. http://dx.doi.org/10.1042/bj20031255.

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In this study, we have presented the first report of Escherichia coli DnaC protein binding to ssDNA (single stranded DNA) in an apparent hexameric form. DnaC protein transfers DnaB helicase onto a nascent chromosomal DNA replication fork at oriC, the origin of E. coli DNA replication. In eukaryotes, Cdc6 protein may play a similar role in the DNA helicase loading in the replication fork during replication initiation at the origin. We have analysed the DNA-binding properties of DnaC protein and a quantitative analysis of the nucleotide regulation of DnaC–DNA and DnaC–DnaB interactions using flu

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Lechman, Eric R., Hidefumi Hiramatsu, Bernhard Gentner, et al. "High Levels of MicroRNA-126 Bioactivity Specify the LSC Compartment in AML." Blood 112, no. 11 (2008): 510. http://dx.doi.org/10.1182/blood.v112.11.510.510.

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Abstract Leukemia stem cells (LSCs) are a biologically distinct blast population positioned at the apex of the acute myeloid leukemia (AML) developmental hierarchy. A more complete understanding of the unique properties of LSCs is crucial for the identification of novel AML regulatory pathways and the subsequent development of innovative therapies that effectively target these cells in leukemia patients. However, most studies overlook the heterogeneity of AML and the existence of LSC, potentially masking important molecular pathways. MicroRNAs (miRNAs) are an emerging class of non-coding small

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Smith, Graham, Habib Alloush, Margaret A. Smith, Philip J. Hill, and Vyvian Salisbury. "A Rapid Assay of Cytosine Arabinoside Uptake and Metabolism by Acute Myeloid Leukaemic Cells Using a Bioluminescent Bacterial Biosensor." Blood 110, no. 11 (2007): 4308. http://dx.doi.org/10.1182/blood.v110.11.4308.4308.

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Abstract We have already reported the construction and evaluation of a self-bioluminescent reporter strain of E. coli with high sensititivity to the nucleoside analogue cytosine arabinoside (Ara-C) the mainstay of treatment in patients with Acute Myeloid Leukaemia (Blood, 106 (11), Abstr. 2473; 695a). This rapid assay, which uses a bioluminescent biosensor to measure intracellular levels of Ara-C and its active metabolite Ara-CTP, has been utilised in leukaemic cell lines KG1a (FAB type M0) which is sensitive to Ara-C and THP-1 (FAB type M5) which is partially insensitive to Ara-C due to over

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Marston, Daniel J., Karen L. Anderson, Mark F. Swift, et al. "High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture." Proceedings of the National Academy of Sciences 116, no. 4 (2019): 1267–72. http://dx.doi.org/10.1073/pnas.1808830116.

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Rac1 activation is at the core of signaling pathways regulating polarized cell migration. So far, it has not been possible to directly explore the structural changes triggered by Rac1 activation at the molecular level. Here, through a multiscale imaging workflow that combines biosensor imaging of Rac1 dynamics with electron cryotomography, we identified, within the crowded environment of eukaryotic cells, a unique nanoscale architecture of a flexible, signal-dependent actin structure. In cell regions with high Rac1 activity, we found a structural regime that spans from the ventral membrane up

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Robinson, Gareth M., Katherine M. Tonks, Robin M. S. Thorn, and Darren M. Reynolds. "Application of Bacterial Bioluminescence To Assess the Efficacy of Fast-Acting Biocides." Antimicrobial Agents and Chemotherapy 55, no. 11 (2011): 5214–19. http://dx.doi.org/10.1128/aac.00489-11.

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ABSTRACTTraditional microbiological techniques are used to provide reliable data on the rate and extent of kill for a range of biocides. However, such techniques provide very limited data regarding the initial rate of kill of fast-acting biocides over very short time domains. This study describes the application of a recombinant strain ofEscherichia coliexpressing thePhotorhabdus luminescens luxoperon as a whole-cell biosensor. Light emission is linked directly to bacterial metabolism; therefore, by monitoring light output, the impact of fast-acting biocides can be assessed. Electrochemically

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Yamamoto, Shinya, Masamichi Yamamoto, Jin Nakamura, et al. "Spatiotemporal ATP Dynamics during AKI Predict Renal Prognosis." Journal of the American Society of Nephrology 31, no. 12 (2020): 2855–69. http://dx.doi.org/10.1681/asn.2020050580.

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BackgroundDepletion of ATP in renal tubular cells plays the central role in the pathogenesis of kidney diseases. Nevertheless, inability to visualize spatiotemporal in vivo ATP distribution and dynamics has hindered further analysis.MethodsA novel mouse line systemically expressing an ATP biosensor (an ATP synthase subunit and two fluorophores) revealed spatiotemporal ATP dynamics at single-cell resolution during warm and cold ischemic reperfusion (IR) with two-photon microscopy. This experimental system enabled quantification of fibrosis 2 weeks after IR and assessment of the relationship bet

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Djenoune, Lydia, Ritu Tomar, Aude Dorison, et al. "Autonomous Calcium Signaling in Human and Zebrafish Podocytes Controls Kidney Filtration Barrier Morphogenesis." Journal of the American Society of Nephrology 32, no. 7 (2021): 1697–712. http://dx.doi.org/10.1681/asn.2020101525.

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BackgroundPodocytes are critical to maintaining the glomerular filtration barrier, and mutations in nephrotic syndrome genes are known to affect podocyte calcium signaling. However, the role of calcium signaling during podocyte development remains unknown.MethodsWe undertook live imaging of calcium signaling in developing podocytes, using zebrafish larvae and human kidney organoids. To evaluate calcium signaling during development and in response to channel blockers and genetic defects, the calcium biosensor GCaMP6s was expressed in zebrafish podocytes. We used electron microscopy to evaluate

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Chen, Po-Yuan, I.-Fen Ko, Kuan-Hsing Chen, et al. "Abstract 3704: A semiconductor-based biosensor for sensitive T cell detection and common neoantigen identification in cancer immunotherapy." Cancer Research 85, no. 8_Supplement_1 (2025): 3704. https://doi.org/10.1158/1538-7445.am2025-3704.

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Abstract Background: Identifying common neoantigens and detecting T cell responses to these antigens are critical for providing therapeutic targets and monitoring treatment efficacy. However, current cancer targeted sequencing panels miss up to 95% of genes corresponding to immunogenic neoantigens, limiting treatment options. Detecting specific T cells responsive to these neoantigens is also challenging due to their low abundance and limitations of existing detection methods. Objective: We developed an innovative extended-gate field-effect transistor (EG-FET) biosensor for sensitive detection

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Gan, Han Ming, Lucas Dailey, Peter Wengert, et al. "Quorum sensing signals of the grapevine crown gall bacterium, Novosphingobium sp. Rr2-17: use of inducible expression and polymeric resin to sequester acyl-homoserine lactones." PeerJ 12 (December 20, 2024): e18657. https://doi.org/10.7717/peerj.18657.

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Background A grapevine crown gall tumor strain, Novosphingobium sp. strain Rr2-17 was previously reported to accumulate copious amounts of diverse quorum sensing signals during growth. Genome sequencing identified a single luxI homolog in strain Rr2-17, suggesting that it may encode for a AHL synthase with broad substrate range, pending functional validation. The exact identity of the complete suite of AHLs formed by novIspR1 is largely unknown. Methods This study validates the function of novIspR1 through inducible expression in Escherichia coli and in the wild-type parental strain Rr2-17. We

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Huang, Min, and Junjie Chen. "Abstract A035: Checkpoint is the control mechanism to ensure the strict order of cellular events during cell cycle. Here, we show that cells enter mitosis without completing DNA replication although with transient activation of known ATR/Chk1 checkpoint." Cancer Research 84, no. 1_Supplement (2024): A035. http://dx.doi.org/10.1158/1538-7445.dnarepair24-a035.

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Abstract Checkpoints are the control mechanisms that ensure the events of the cell cycle strictly ordered into dependent pathways. Notably, genome duplication and chromosome segregation are two major events of every cell cycle, which respectively occur in S phase and mitosis (M). Checkpoint either for the S/G2 or G2/M transition is considered of great importance to ensure the completion of DNA replication before the initiation of mitosis. Moreover, the degradation tag (dTAG) system allows precise and well-controlled analysis of essential genes by inducing immediate degradation of the protein o

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Antony-Debre, Ileana, Ananya Paul, Joana Leite, et al. "Direct Pharmacological Inhibition of the Transcription Factor PU.1 in Acute Myeloid Leukemia." Blood 130, Suppl_1 (2017): 858. http://dx.doi.org/10.1182/blood.v130.suppl_1.858.858.

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Abstract Functionally critical decreases in levels or activity of the ETS family transcription factor PU.1 are present in approximately 2/3 of patients with acute myeloid leukemia (AML), across different AML subtypes (Sive, Leukemia 2016) including at the stem cell level (Steidl, Nat Genet 2006; Will, Nat Med 2015). Thus, targeting PU.1 could be an appealing option for treatment. As complete loss of PU.1 leads to stem cell failure (Iwasaki, Blood 2005), we hypothesized that PU.1 inhibition could eradicate leukemic cells harboring already low levels of PU.1, with modest effects on normal cells.

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Frey, Wesley D., Ashlyn Y. Anderson, Hyemin Lee, et al. "Phosphoinositide species and filamentous actin formation mediate engulfment by senescent tumor cells." PLOS Biology 20, no. 10 (2022): e3001858. http://dx.doi.org/10.1371/journal.pbio.3001858.

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Cancer cells survive chemotherapy and cause lethal relapse by entering a senescent state that facilitates expression of many phagocytosis/macrophage-related genes that engender a novel cannibalism phenotype. We used biosensors and live-cell imaging to reveal the basic steps and mechanisms of engulfment by senescent human and mouse tumor cells. We show filamentous actin in predator cells was localized to the prey cell throughout the process of engulfment. Biosensors to various phosphoinositide (PI) species revealed increased concentration and distinct localization of predator PI(4) P and PI(4,5

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Rotureau, Elise, Christophe Pagnout, and Jérôme F. L. Duval. "Physicochemical Rationale of Matrix Effects Involved in the Response of Hydrogel-Embedded Luminescent Metal Biosensors." Biosensors 14, no. 11 (2024): 552. http://dx.doi.org/10.3390/bios14110552.

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There is currently a critical need for understanding how the response and activity of whole-cell bacterial reporters positioned in a complex biological or environmental matrix are impacted by the physicochemical properties of their micro-environment. Accordingly, a comprehensive analysis of the bioluminescence response of Cd(II)-inducible PzntA-luxCDABE Escherichia coli biosensors embedded in silica-based hydrogels is reported to decipher how metal bioavailability, cell photoactivity and ensuing light bioproduction are impacted by the hydrogel environment and the associated matrix effects. The

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LIU, YONGCHENG, JIANMING YE, and YANBIN LI. "Rapid Detection of Escherichia coli O157:H7 Inoculated in Ground Beef, Chicken Carcass, and Lettuce Samples with an Immunomagnetic Chemiluminescence Fiber-Optic Biosensor." Journal of Food Protection 66, no. 3 (2003): 512–17. http://dx.doi.org/10.4315/0362-028x-66.3.512.

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A biosensor was evaluated with regard to its usefulness in the rapid detection of Escherichia coli O157:H7 inoculated in ground beef, chicken carcass, and romaine lettuce samples. The biosensor consisted of a chemiluminescence reaction cell, a fiber-optic light guide, and a luminometer linked to a personal computer in conjunction with immunomagnetic separation. The samples inoculated with E. coli O157:H7 were first centrifuged and suspended in buffered peptone water and then incubated with anti–E. coli O157 antibody–coated magnetic beads and horseradish peroxidase(HRP)–labeled anti–E. coli O15

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XU, YING, HUI YU, HUA CAI, QINGJUN LIU, and PING WANG. "A NOVEL INTEGRATED CELL-BASED BIOSENSOR FOR EXTRACELLULAR ELECTROPHYSIOLOGICAL MONITORING." International Journal of Information Acquisition 04, no. 03 (2007): 193–204. http://dx.doi.org/10.1142/s0219878907001289.

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A rigorous preselection of identified compounds by in vitro cellular screening is necessary prior to using the drug candidates for the further time consuming and parallel monitoring stage. In this paper we introduced an integrated cell based chip which can detect changes of electrophysiological parameters by using microelectronic chips such as Light-Addressable Potentiometric Sensors (LAPS), Micro-electrodes Array Sensors (MEAS) and Interdigitated Array Sensor (IDA) etc. to record the extracellular metabolism chemical and biological substances, action potential and living characteristics of ce

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Cole, Michael, Nikhil Ranjan, Daniel G. Flores, et al. "Optimizing Complement Inhibitor Monitoring in PNH and Beyond." Blood 144, Supplement 1 (2024): 4071. https://doi.org/10.1182/blood-2024-200838.

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Introduction: There are now 6 FDA approved therapies for paroxysmal nocturnal hemoglobinuria (PNH). All are effective in controlling complement-mediated intravascular hemolysis (IVH) but associated with potential for breakthrough hemolysis (BTH). Differentiating pharmacokinetic (PK) from pharmacodynamic BTH and defining the exact contribution of extravascular hemolysis (EVH) to persistent anemia remains an ongoing diagnostic challenge. Outside the CH50 for eculizumab, there are no clinically available tests for monitoring of complement blockade. CH50 has variable accuracy for ravulizumab monit

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Cui, Yi, Xinjie Chen, Ze Wang, and Yuan Lu. "Cell-Free PURE System: Evolution and Achievements." BioDesign Research 2022 (September 1, 2022): 1–11. http://dx.doi.org/10.34133/2022/9847014.

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The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an in vitro open environment without a complete living cell. It has been widely used in basic and applied research fields because of its advanced engineering features in flexibility and controllability. Compared to a typical crude extract-based CFPS system, due to defined and customizable components and lacking protein-degrading enzymes, the protein synthesis using recombinant elements (PURE) system draws great attention. This review first discusses

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Choi, Hye Kyu, Jin-Ha Choi, and Jinho Yoon. "An Updated Review on Electrochemical Nanobiosensors for Neurotransmitter Detection." Biosensors 13, no. 9 (2023): 892. http://dx.doi.org/10.3390/bios13090892.

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Neurotransmitters are chemical compounds released by nerve cells, including neurons, astrocytes, and oligodendrocytes, that play an essential role in the transmission of signals in living organisms, particularly in the central nervous system, and they also perform roles in realizing the function and maintaining the state of each organ in the body. The dysregulation of neurotransmitters can cause neurological disorders. This highlights the significance of precise neurotransmitter monitoring to allow early diagnosis and treatment. This review provides a complete multidisciplinary examination of

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Chew, Teng-Leong, Wendy A. Wolf, Patricia J. Gallagher, Fumio Matsumura, and Rex L. Chisholm. "A fluorescent resonant energy transfer–based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows." Journal of Cell Biology 156, no. 3 (2002): 543–53. http://dx.doi.org/10.1083/jcb.200110161.

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Approaches with high spatial and temporal resolution are required to understand the regulation of nonmuscle myosin II in vivo. Using fluorescence resonance energy transfer we have produced a novel biosensor allowing simultaneous determination of myosin light chain kinase (MLCK) localization and its [Ca2+]4/calmodulin-binding state in living cells. We observe transient recruitment of diffuse MLCK to stress fibers and its in situ activation before contraction. MLCK is highly active in the lamella of migrating cells, but not at the retracting tail. This unexpected result highlights a potential ro

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Mauzeroll, Janine. "Neurologically Relevant Enzyme Expression and Engineering for D-Amino Acid Enzymatic Electrochemical Biosensor Development." ECS Meeting Abstracts MA2019-02, no. 55 (2019): 2428. http://dx.doi.org/10.1149/ma2019-02/55/2428.

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Our interest lies in using enzymes to develop biosensors to be employed in studying neurochemical processes occurring in the brain. We will discuss expression and characterization of three different wild type enzymes and their mutants (Glycine oxidase, D-aspartate oxidase, D-amino acid oxidase). Enzyme expression and purification will be completed through standard biochemistry techniques while activity characterization will be assessed using spectrophotometric assays. Upon expression and characterization, these enzymes will then be used to develop enzymatic bioelectrochemical sensors which wil

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Tonino, Rik Paulus Bernardus, Karen Larimer, Okke Eissen, and Martin Roelof Schipperus. "Remote Patient Monitoring in Adults Receiving Transfusion or Infusion for Hematological Disorders Using the VitalPatch and accelerateIQ Monitoring System: Quantitative Feasibility Study." JMIR Human Factors 6, no. 4 (2019): e15103. http://dx.doi.org/10.2196/15103.

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Background Frequent vital sign monitoring during and after transfusion of blood products and certain chemotherapies or immunotherapies is critical for detecting infusion reactions and treatment management in patients. Currently, patients return home with instructions to contact the clinic if they feel unwell. Continuous monitoring of vital signs for hematological patients treated with immunotherapy or chemotherapy or receiving blood transfusions using wearable electronic biosensors during and post treatment may improve the safety of these treatments and make remote data collection in an outpat

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Tawil, Stéphane, Loic Leroy, Martial Billon, Neso Sojic, and Aurélie Bouchet-Spinelli. "Electrochemiluminescence in “Planar” Micropore for Application in Single Cell Cytokine Biosensing." ECS Meeting Abstracts MA2024-01, no. 42 (2024): 2370. http://dx.doi.org/10.1149/ma2024-01422370mtgabs.

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The immune response is a complex process involving interactions mediated by cellular secretions (cytokines). These key proteins dictate each cell’s behavior and are thus important markers to decipher the immune response. Produced in very low quantities, at the single cell level, their efficient detection is still a bottleneck for the complete understanding of the immune process. Since the early 2000s, biosensors based on micro and nanopores have emerged for the detection and identification of biomolecules [1]. However, the main challenge of such biosensors remains the appropriate functionaliza

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Frey, Wesley D., Ashlyn Anderson, Julie Nguyen, Emma Cowles, and James Jackson. "Abstract 1576: Chemotherapy induced cellular cannibalism is mediated by phosphoinositide species and clathrin." Cancer Research 82, no. 12_Supplement (2022): 1576. http://dx.doi.org/10.1158/1538-7445.am2022-1576.

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Abstract Cancer cells can survive chemotherapy and drive lethal relapse if they avoid cell death in conditions of 1) DNA damage and/or mitotic stress caused by treatment; 2) nutrient depletion. We and others have previously shown that the breast cancers most likely to survive chemotherapy are TP53 wild-type, and these are among the most lethal breast cancers. For instance, chemotherapy treated TNBC patients with TP53 wild-type tumors have a median overall survival of 45 months, contrasting with 263 months for TP53 mutant tumors. TP53 wild type cells survive stress by entering a state of arrest

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Cole, Michael, Nikhil Ranjan, Gloria F. Gerber, et al. "Complement Biosensors Can be Used to Identify Classical Pathway and Alternative Pathway Dysregulation in Complement-Mediated Thrombotic Microangiopathy." Blood 144, Supplement 1 (2024): 1225. https://doi.org/10.1182/blood-2024-210885.

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Complement-mediated thrombotic microangiopathy or hemolytic uremic syndrome (CM-TMA/CM-HUS) is a thrombotic microangiopathy commonly characterized by germline variants or acquired antibodies to complement proteins and regulators. However, these variants are neither necessary nor sufficient, and many patients are found to have variants of undetermined significance complicating definitive diagnosis. New tools are needed to provide a global assessment of complement dysregulation and better differentiate TMA into categories that might predict response to complement inhibitor therapies. We develope

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Schaaf, Tory M., Kurt C. Peterson, Benjamin D. Grant, David D. Thomas, and Gregory D. Gillispie. "Spectral Unmixing Plate Reader: High-Throughput, High-Precision FRET Assays in Living Cells." SLAS DISCOVERY: Advancing the Science of Drug Discovery 22, no. 3 (2016): 250–61. http://dx.doi.org/10.1177/1087057116679637.

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We have developed a microplate reader that records a complete high-quality fluorescence emission spectrum on a well-by-well basis under true high-throughput screening (HTS) conditions. The read time for an entire 384-well plate is less than 3 min. This instrument is particularly well suited for assays based on fluorescence resonance energy transfer (FRET). Intramolecular protein biosensors with genetically encoded green fluorescent protein (GFP) donor and red fluorescent protein (RFP) acceptor tags at positions sensitive to structural changes were stably expressed and studied in living HEK cel

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Rajsri, Kritika Srinivasan, Michael P. McRae, Nicolaos J. Christodoulides, et al. "Abstract 6087: Cytomics-on-chip and AI-driven predictive analysis platform for early detection of epithelial cancers." Cancer Research 84, no. 6_Supplement (2024): 6087. http://dx.doi.org/10.1158/1538-7445.am2024-6087.

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Abstract Cancer is the 2nd leading cause of death (over 605,000 people) in the US, at an expense of over $200B, with 1 in 3 people projected to have cancer during their lifetime per CDC. Despite the significant impact of early detection and screening on prognosis, only some cancers are diagnosed at an early stage. Carcinomas, comprising &gt;80% of cancer incidence, allow ease in cytology sample access chairside, due to the lesions’ epithelial presentation. This presents a unique opportunity for early detection and screening in epithelial cancers. In low-resource healthcare settings, from c

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Murthy, Vaibhav, Cemal Erdem, Jeremy Copperman, Marc Birtwistle, and Alexander Davies. "Abstract 5535: Intracellular negative regulators of RTK-Ras-ERK signaling alter breast cancer perception of metastatic niche-derived growth factors." Cancer Research 84, no. 6_Supplement (2024): 5535. http://dx.doi.org/10.1158/1538-7445.am2024-5535.

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Abstract Triple negative breast cancer (TNBC) is an aggressive breast cancer subtype that commonly metastasizes to distant organs, such as the lung, resulting in the poor clinical outcomes. TNBC cells commonly overexpress epidermal growth factor receptor (EGFR), a receptor tyrosine kinase (RTK) which when bound by microenvironmental growth factors, results in the activation of downstream effector kinases ERK-AKT and initiates transcriptional programs that control cell fate. Tumor cells, however, simultaneously receive many growth factor inputs from their respective microenvironments, many of w

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Born, Friederike, Peter Braun, Holger C. Scholz, and Gregor Grass. "Specific Detection of Yersinia pestis Based on Receptor Binding Proteins of Phages." Pathogens 9, no. 8 (2020): 611. http://dx.doi.org/10.3390/pathogens9080611.

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The highly pathogenic bacterium Yersinia pestis is the causative agent of plague, a notorious infectious zoonotic disease. When transmitted from person to person as pneumonic plague via droplets, Y. pestis is highly contagious and in most cases is fatal if left untreated. Thus, when plague is suspected, rapid diagnosis is crucial, as a serious course of the infection is only averted by early antibiotic therapy. The bacterium is easy to cultivate, accessible and has a high potential for nefarious use such as bioterrorism. Highly specific, rapid and easy-to-use confirmatory diagnostic methods ar

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Zhang, Yang, Pablo J. Dopico, Minh-Chau N. Le, et al. "Abstract 2315: Temporal changes in circulating tumor cells and circulating tumor DNA in patients with resectable pancreatic ductal adenocarcinoma." Cancer Research 83, no. 7_Supplement (2023): 2315. http://dx.doi.org/10.1158/1538-7445.am2023-2315.

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Abstract Background: Circulating tumor cells (CTCs), circulating cell-free DNA (cfDNA), and circulating tumor DNA (ctDNA) are minimally invasive biomarkers that carry vital tumor onset, progression, and metastasis information, which is crucial for cancer diagnosis, prognosis, and management. We report here our longitudinal study of these circulating biomarkers in resectable pancreatic ductal adenocarcinoma (PDAC) patients. Although the overall 5-year survival rate of PDAC is low (~6%), truly resectable patients are expected to have better outcomes than non-resectable PDAC patients, especially

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Lo, Ting-Wen, Yuqian Zhang, Jing Liu, et al. "Abstract A030: Rapid extraction and detection of extracellular vesicle-derived PD-L1 in a microfluidic platform." Clinical Cancer Research 30, no. 21_Supplement (2024): A030. http://dx.doi.org/10.1158/1557-3265.liqbiop24-a030.

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Abstract Background. Extracellular vesicles (EV) are emerging as new biomarkers for cancer diagnostics and therapeutic monitoring1. For example, in patients that receive PD1/PD-L1 immune checkpoint inhibitors (a key pillar of cancer immunotherapy), circulating EV-derived PD-L1 is gaining attention as a non-invasive biomarker for therapeutic efficacy. Standard methodologies to measure EV-derived PD-L1 requires ultracentrifugation to first separate EVs and then quantify those that express PD-L1 using nano flow cytometry. These are tedious processes that require trained technologists in centraliz

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Foroughi, Shervin, Iris Handa, and Muthukumaran Packirisamy. "3D Printed Single Mold Multi-Level (SMILE) Interconnected Microfluidic Chip for Droplet Generation." ECS Meeting Abstracts MA2025-01, no. 62 (2025): 3056. https://doi.org/10.1149/ma2025-01623056mtgabs.

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Droplet microfluidics is essential for applications such as droplet formation1, particle synthesis2, and cell and gene manipulation3. Most droplet generators use flow-focusing technology in rectangular microchannels4, typically fabricated via lithography from polydimethylsiloxane (PDMS), polymers, or glass. However, planar designs limit chip size and create alignment challenges for inner and outer capillaries4. To address these limitations, multilevel soft lithography can be used to create non-planar channel configurations, such as 3D mixers with multi-layered microchannels for improved diffus

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Niu, Alex, Bo Ning, Francisco Socola, et al. "COVID-19 in Patients with Hematological Malignancies: High False Negative Rate with High Mortality." Blood 136, Supplement 1 (2020): 6–7. http://dx.doi.org/10.1182/blood-2020-138611.

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Introduction Patients with hematological malignancies (HM) are uniquely immunocompromised and considered at high risk for COVID-19. However, data regarding the diagnosis, clinical course, treatment, and outcomes of these patients is sparse. In particular, the ability to successfully detect SARS-CoV-2 in patients with HM remains unknown. We have previously reported 2 cases of allogeneic stem cell transplant (SCT) diagnosed with COVID-19 using clustered regularly interspaced short palindromic repeats (CRISPR) technique, following multiple negative nasopharyngeal RT-PCR testing (Niu et al. Bone M

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Erbaş, Aşkın, Selinsu Dikim, Fatma Arslan, et al. "Schiff Bases From 4‐Aminoantipyrine: Investigation of Their In Silico, Antimicrobial, and Anticancer Effects and Their Use in Glucose Biosensor Design." Bioinorganic Chemistry and Applications 2025, no. 1 (2025). https://doi.org/10.1155/bca/2786064.

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Five new Schiff bases from 4‐aminoantipyrine were synthesized, characterized, and evaluated for their antimicrobial and DNA cleavage activities, and drug similarity properties and cytotoxicity prediction using in silico analysis. All Schiff bases had good antibacterial and antifungal activities. All compounds showed self‐activating DNA cleavage ability in the absence of any reductant or oxidant at low concentrations. Modified carbon paste electrodes were prepared with all Schiff bases, and a glucose biosensor was designed. Schiff base coded (4AA‐Fc) was found to have the best sensitivity to H2

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MELNYK, OLHA, CHARANYA MURALIDHARAN, MICHELLE M. MARTINEZ IRIZARRY, JUSTIN CROWDER, and AMELIA K. LINNEMANN. "1443-P: Real-Time In Vivo Analysis of Beta-Cell Autophagy in Autoimmune Diabetes." Diabetes 71, Supplement_1 (2022). http://dx.doi.org/10.2337/db22-1443-p.

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Type 1 Diabetes (T1D) is an autoimmune disease caused by a combination of genetic and environmental factors. The onset of disease is associated with autoimmune attack against pancreatic beta-cells leading to a reduction of their number. While a role for the beta-cell in these events is widely hypothesized, a complete understanding of disease pathogenesis is still lacking. Our group recently found that beta-cell autophagy is defective in the context of human T1D as well as in the non-obese diabetic (NOD) mouse model of autoimmune diabetes. Importantly, our data suggested that defective autophag

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Li, Min, Zhenya Chen, Wuyuan Zhang, Tong Wu, Qingsheng Qi, and Yi‐Xin Huo. "Customization of Ethylene Glycol (EG)‐Induced BmoR‐Based Biosensor for the Directed Evolution of PET Degrading Enzymes." Advanced Science, February 10, 2025. https://doi.org/10.1002/advs.202413205.

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AbstractThe immense volume of plastic waste poses continuous threats to the ecosystem and human health. Despite substantial efforts to enhance the catalytic activity, robustness, expression, and tolerance of plastic‐degrading enzymes, the lack of high‐throughput screening (HTS) tools hinders efficient enzyme engineering for industrial applications. Herein, we develop a novel fluorescence‐based HTS tool for evolving polyethylene terephthalate (PET) degrading enzymes by constructing an engineered BmoR‐based biosensor targeting the PET breakdown product, ethylene glycol (EG). The EG‐responsive bi

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Padmaraj, Divya, Rohit Pande, Wanda Zagozdzon-Wosik, et al. "Biosensor for Dielectric Spectroscopy of Mitochondria and for Monitoring Ion Activities." MRS Proceedings 1236 (2009). http://dx.doi.org/10.1557/proc-1236-ss01-10.

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AbstractWe developed a BioMEMS device to study cell- mitochondrial physiological functionalities. The pathogenesis of many diseases including obesity, diabetes, heart failure as well as aging has been linked to functional defects of mitochondria. This is understandable as the mitochondria produces up to 90% of ATP, and plays a critical role in cell signaling and apoptosis. The synthesis of ATP is determined by the electrical potential across the inner mitochondrial membrane (IMM) and by the pH difference due to proton flux across it. Therefore, electrical characterization by E-fields with comp

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