To see the other types of publications on this topic, follow the link: Complexes CTC.
Journal articles on the topic 'Complexes CTC'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Complexes CTC.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Kancono, N., and H. B. Senin. "Gelification Effects on the Structure and Birefringence of Charge Transfer Complex Terthiophene Bisililated – TCNQ Hybrid Materials." Materials Science Forum 517 (June 2006): 257–61. http://dx.doi.org/10.4028/www.scientific.net/msf.517.257.
Full textAbstract:
Charge transfer complexes (CTC) can be readily introduced into materials by cohydrolysis-copolymerisation of bis-silylated ter-thiophenes as precursors with TMOS and TEOS in the presence of TCNQ. CTC formation is shown in the visible spectrum of the xerogel by the band at 850 nm characteristic of the TCNQ·- radical anion. Vibrational spectra have shown that strong vibration of C≡N bond at 2184, 2120 and 1595 cm-1 as peaks characteristics of CTC. The CTC bands are weak and the complex is easily destroyed by washing with acetone, which removes the TCNQ. The gelification effect of the charge transfer complexes on the hybrid materials of 2,5’’- bis(trime thoxysilyl)terthiophene/TCNQ/ TMOS showed that the peak with distance of more than 11.68 Å, formed by precursors and matrices, as a lamellar structure. The birefringence of xerogel BTS3T in presence of alkoxysilane showed that the value is near the detection limit of 0.1 – 0.4 x 10-3, which is weaker than BTS3T / THF with the birefringence value of 4.5 x 10-3.
2
Chen, Hong, Mehdi Vahdati, Pu Xiao, Frédéric Dumur, and Jacques Lalevée. "Water-Soluble Visible Light Sensitive Photoinitiating System Based on Charge Transfer Complexes for the 3D Printing of Hydrogels." Polymers 13, no. 18 (September 21, 2021): 3195. http://dx.doi.org/10.3390/polym13183195.
Full textAbstract:
The development of visible-light 3D printing technology by using water-soluble initiating systems has attracted widespread attention due to their potential applications in the manufacture of hydrogels. Besides, at present, the preparation of water-soluble photoinitiators suitable for visible light irradiation (such as LEDs) still remains a challenge. Therefore, this work is devoted to developing water-soluble photoinitiators (PI)/photoinitiating systems (PIS) upon irradiation with a LED @ 405 nm. In detail, a new water-slightly-soluble chalcone derivative dye [(E)-3-(4-(dimethylamino) phenyl)-1-(4-(2-(2-(2-methoxyethoxy) ethoxy) ethoxy) phenyl) prop-2-en-1-one] was synthesized here and used as a PI with a water-soluble coinitiator, i.e., triethanolamine (TEA) which was also used as an electron donor. When combined together, a charge transfer complex (CTC) formed immediately which exhibited excellent initiating ability for the free radical photopolymerization of poly(ethyleneglycol)diacrylate (PEG-DA). In light of the powerful CTC effect, the [dye-TEA] CTC could not only exhibit enhanced water solubility and mechanical properties but could also be effectively applied for 3D printing. This CTC system is environmentally friendly and cost-saving which demonstrates a great potential to prepare hydrogels via photopolymerization.
3
Loughrey, Jonathan J., Colin A. Kilner, Michaele J. Hardie, and Malcolm A. Halcrow. "Six new crystalline clathrates of cyclotricatechylene (CTC) including two donor–acceptor complexes." Supramolecular Chemistry 24, no. 1 (October 7, 2011): 2–13. http://dx.doi.org/10.1080/10610278.2011.611246.
Full text
4
Crémoux, T., I. Batonneau-Gener, A. Moissette, J. L. Paillaud, M. Hureau, E. Ligner, C. Morais, S. Laforge, C. Marichal, and H. Nouali. "Influence of framework Si/Al ratio and topology on electron transfers in zeolites." Physical Chemistry Chemical Physics 21, no. 27 (2019): 14892–903. http://dx.doi.org/10.1039/c9cp01166h.
Full textAbstract:
From experimental results on H-ZSM-5 and H-*BEA zeolites, it is shown that the stability of radical cations and of charge transfer complexes (CTC) is highly dependent on the distance between Brønsted sites and strong Lewis sites or Brønsted Strong Lewis Pairs (BSLPs).
5
Shen, Dong, Yan Wu, Ming-Fai Lo, and Chun-Sing Lee. "Charge transport properties of co-evaporated organic–inorganic thin film charge transfer complexes: effects of intermolecular interactions." Journal of Materials Chemistry C 8, no. 47 (2020): 16725–29. http://dx.doi.org/10.1039/d0tc04278a.
Full textAbstract:
Charge transport properties of the organic–inorganic MoO3 : 6T CTC thin film can be dramatically tuned via rubbing. The effects of intermolecular interactions on these changes are studied in detail.
6
Demirhan, Hulya, Mustafa Arslan, Mustafa Zengin, and Mustafa Kucukislamoglu. "Investigation of Charge Transfer Complexes Formed between Mirtazapine and Someπ-Acceptors." Journal of Spectroscopy 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/875953.
Full textAbstract:
Charge transfer complexes (CTC) of mirtazapine with tetracyanoethylene (TCNE), 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ), and tetracyanoquinodimethane (TCNQ) have been studied spectrophotometrically in dichloromethane at room temperature. The stoichiometries of the complexes were found to be 1 : 1 ratio by the Job Method between mirtazapine and the acceptors. The equilibrium constants and thermodynamic parameters of the complexes were determined by the Benesi-Hildebrand and Van't Hoff equations. Mirtazapine in pure and dosage form was applied in this study. The results indicate that the formation constants for the complexes depend on the nature of electron acceptors and donor. And also the spectral studies of the complexes were determined by FT-IR and NMR spectroscopy.
7
LEVAVASSEUR, Françoise, Jocelyne LIÉTARD, Kohei OGAWA, Nathalie THÉRET, Peter D. BURBELO, Yoshihiko YAMADA, André GUILLOUZO, and Bruno CLÉMENT. "Expression of laminin γ 1 cultured hepatocytes involves repeated CTC and GC elements in the LAMC1 promoter." Biochemical Journal 313, no. 3 (February 1, 1996): 745–52. http://dx.doi.org/10.1042/bj3130745.
Full textAbstract:
Laminin γ1 chain is present in all basement membranes and is expressed at high levels in various diseases, such as hepatic fibrosis. We have identified cis- and trans-acting elements involved in the regulation of this gene in normal rat liver, as well as in hepatocyte primary cultures and hepatoma cell lines. Northern-blot analyses showed that laminin γ1 mRNA was barely detectable in freshly isolated hepatocytes and expressed at high levels in hepatocyte primary cultures, as early as 4 h after liver dissociation. Actinomycin D and cycloheximide treatment in vivo and in vitro indicated that laminin γ1 overexpression in cultured hepatocytes was under the control of transcriptional mechanisms. Transfection of deletion mutants of the 5´ flanking region of murine LAMC1 gene in hepatoma cells that constitutively express laminin γ1 indicated that regulatory elements were located between -594 bp and -94 bp. This segment included GC- and CTC-containing motifs. Gel-shift analyses showed that two complexes were resolved with different affinity for the CTC sequence depending on the location of the GC box. The pattern of complex formation with nuclear factors from freshly isolated and cultured hepatocytes was different from that obtained with total liver and similar to that with hepatoma cells. Southwestern analysis indicated that several polypeptides bound the CTC-rich sequence. Affinity chromatography demonstrated that a Mr 60000 polypeptide was a major protein binding to the CTC motif. This polypeptide is probably involved in the transcriptional activation of various proto-oncogenes and extracellular matrix genes that are expressed at high levels in both hepatoma cells and early hepatocyte cultures.
8
Zapunidy, Sergey A., Dmitry S. Martyanov, Elena M. Nechvolodova, Marina V. Tsikalova, Yuri N. Novikov, and Dmitry Yu Paraschuk. "Approaches to low-bandgap polymer solar cells: Using polymer charge-transfer complexes and fullerene metallocomplexes." Pure and Applied Chemistry 80, no. 10 (January 1, 2008): 2151–61. http://dx.doi.org/10.1351/pac200880102151.
Full textAbstract:
Polymer solar cells have shown high potential to convert solar energy into electricity in a cost-effective way. One of the basic reasons limiting the polymer solar cell efficiency is insufficient absorption of the solar radiation by the active layer that limits the photocurrent. To increase the photocurrent, one needs low-bandgap materials with strong absorption below 2 eV. In this work, we study two types of low-bandgap materials: ground-state charge-transfer complexes (CTCs) of a conjugated polymer, MEH-PPV (poly[2-methoxy-5-(2'-ethyl-hexyloxy)-1,4-phenylenevinylene]), and an exohedral metallocomplex of fullerene, (η2-C60)IrH(CO)[(+)DIOP] (IrC60). We demonstrate that the CTC formed between MEH-PPV and conjugated molecules with high electron affinity, namely, 2,4,7-trinitrofluorenone (TNF) and 1,5-dinitroantraquinone (DNAQ), can have strong optical absorption extending down to the near infrared. We have observed that the photoexcited CTC can generate free charges. We also report on optical studies of IrC60 as a possible acceptor for polymer/fullerene solar cells. IrC60 strongly absorbs in the visible spectral range, in particular in the red part, and therefore has a potential for increasing the photocurrent as compared with polymer/methanofullerene solar cells. Our studies of MEH-PPV/IrC60 blended films show that long-lived charges are efficiently generated at MEH-PPV upon photoexcitation of the blend.
9
Mohamdi, Messaouda, Nadjia Bensouilah, and Mohamed Abdaoui. "Investigation of charge transfer complexes formed between (S, S)-bis-N,N-sulfonyl bis-L-phenylalanine dimethylester donor with tetracyanoethylene and chloranil as π-acceptors: Experimental and DFT studies." Journal of Theoretical and Computational Chemistry 15, no. 01 (February 2016): 1650009. http://dx.doi.org/10.1142/s0219633616500097.
Full textAbstract:
Two novel charge transfer complexes CTC ([D[Formula: see text]TCNE] and [D[Formula: see text]CHL] : D [Formula: see text] (S, S)-bis-N,N-sulfonyl bis-L-phenylalanine dimethylester; TCNE [Formula: see text] Tetracyanoethylene; CHL [Formula: see text] Chloranil) were synthesized and characterized by elemental analysis: Electronic absorption, spectrophotometric titration, IR. The obtained results indicate the formation of 1:1 for both complexes. The experimental studies were complemented by quantum chemical calculations at DFT/CAM-B3LYP level of theory. Optimized geometrical structures, the electronic spectroscopy, excited-state properties and the descriptions of frontier molecular orbitals were computed and discussed by time-dependent density functional theory (TD-DFT). In addition, vibrational frequency calculations, the natural population analysis (NPA) confirms the presence of intermolecular interactions and natural bonding orbitals (NBO) calculation was carried out in order to elucidate the interactions between TCNE [Formula: see text]-acceptor and donor molecule.
10
Liu, Jiamin, Jianjun Li, Juan Liang, Chengjun Jing, and Jiaxiu Guo. "Synergistic effect of citric acid and carbon dots modified g-C3N4 for enhancing photocatalytic reduction of Cr(VI)." Journal of Water Supply: Research and Technology-Aqua 70, no. 4 (April 7, 2021): 570–86. http://dx.doi.org/10.2166/aqua.2021.163.
Full textAbstract:
Abstract Carbon dot (CD)-modified graphitic carbon nitride (g-C3N4) photocatalysts were synthesized through a one-step homogeneous thermal pyrolysis. The synergetic effect of citric acid (Cit) and g-C3N4/CDs for high-performance visible light Cr(VI) photocatalytic reduction had been investigated. Cit was not only acted as a hole scavenger, but might also form surface charge transfer complexes (CTC) with g-C3N4 which delivered electrons on the Highest Occupied Molecular Orbital (HOMO) of Cit to the conduction band (CB) of g-C3N4. CDs decorated on g-C3N4 could provide channels for the preferential transfer of electrons on CTC to the CB of g-C3N4 as well as improved separation of the charge carriers. Owing to these synergistic effects, g-C3N4/CDs displayed much higher photocatalytic performance for the reduction of Cr(VI), which was 1.89 times higher than g-C3N4. Moreover, the synergetic photocatalytic reduction mechanisms of aqueous Cr(VI) were proposed to elucidate the active species formation and photogenerated electron transfer. The results suggested that the in situ generated hydrogen peroxide (H2O2) dominated the reduction of Cr(VI). The addition of Cit could trigger the in situ generation of H2O2 and the decorated CDs further enhanced the reaction. This work demonstrated the role of widely existed Cit on the photocatalytic reduction of Cr(VI) in natural aquatic environment.
11
Chi, Kim N., Sebastien J. Hotte, Susan Ellard, Joel Roger Gingerich, Anthony Michael Joshua, Evan Y. Yu, and Martin Edwin Gleave. "A randomized phase II study of OGX-427 plus prednisone (P) versus P alone in patients (pts) with metastatic castration resistant prostate cancer (CRPC)." Journal of Clinical Oncology 30, no. 15_suppl (May 20, 2012): 4514. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.4514.
Full textAbstract:
4514 Background: Heat Shock Protein 27 (Hsp27) is a multi-functional chaperone protein that regulates cell signaling and survival pathways implicated in cancer progression. In prostate cancer models, Hsp27 complexes with androgen receptor (AR) and enhances transactivation of AR-regulated genes. OGX-427 is a 2nd generation antisense oligonucleotide that inhibits Hsp27 expression with in vitro and in vivo efficacy and was well tolerated with single agent activity in phase I studies. Methods: Chemotherapy-naïve pts with no/minimal symptoms were randomized to receive OGX-427 600 mg IV x 3 loading doses then 1000 mg IV weekly with P 5 mg PO BID or P only. Primary endpoint was the proportion of pts progression free (PPF) at 12 weeks (PCWG2 criteria). A 2-stage MinMax design (H0 = 5%, HA >20%, α=0.1, β=0.1) with 32 pts/arm provides 70% power to detect the difference at 0.10 1-sided significance. Secondary endpoints include PSA decline, measurable disease response, and circulating tumour cell (CTC) enumeration. Results: 38 pts have been enrolled; 1st stage of accrual completed with 2nd stage accruing. In the 1st 32 pts randomized (17 to OGX-427+P, 15 to P), baseline median age was 71 years (53-89), ECOG PS 0 or 1 in 66% and 34% of pts, median PSA 66 (6-606), metastases in bone/lymph nodes/liver or lung was 75/56/9%, 31% had prior P treatment, and 93% had ≥5 CTC/7.5 ml. Predominantly grade 1/2 infusion reactions (chills, diarrhea, flushing, nausea, vomiting) occurred in 47% of pts receiving OGX-427+P. One pt on OGX-427+P developed hemolytic uremic syndrome. A PSA decline of ≥50% occurred in 41% of pts on OGX-427+P, and 20% of pts treated with P. A measurable disease partial response was seen in 3/8 (38%) evaluable pts on OGX-427+P and 0/9 pts on P. CTC conversion from ≥5 to <5/7.5 ml occurred in 50% of pts on OGX-427+P and 31% treated with P. Thus far, in 26 evaluable pts the PPF at 12 weeks was 71% (95% CI: 42-92) in OGX-427+P treated pts and 33% (95% CI: 10-65) in pts on P. Conclusions: These data provide clinical evidence for the role of Hsp27 as a therapeutic target in prostate cancer and support continued evaluation of OGX-427 for pts with CRPC. Funded by a grant from the Terry Fox Research Institute.
12
Fees, Jörg, H. D. Hausen, and Wolfgang Kaim. "Molekül- und Kristallstrukturen zweier einkerniger Ruthenium(II)-Komplexe mit dem starken π·Akzeptorliganden 2,2'-Azobis(pyridin) (abpy): [mer-Ru(abpy)3](PF6)2 und Ru(abpy)2Cl2 (ctc-Isomer) / Molecular and Crystal Structures of Two Mononuclear Ruthenium(II) Complexes with the Strongly π Accepting Ligand 2,2'-Azobis(pyridine) (abpy): [mer-Ru(abpy)3](PF6)2 and Ru(abpy)2Cl2 (ctc Isomer)." Zeitschrift für Naturforschung B 50, no. 1 (January 1, 1995): 15–22. http://dx.doi.org/10.1515/znb-1995-0104.
Full textAbstract:
Crystal structure analyses of the title complexes have been carried out in order to establish their molecular configurations. The tris(chelate) complex dication in (1)(PF6)2 exhibits a mer configuration of pyridine and azo nitrogen atoms in an approximately octahedral arrangement at the metal. The dichlororuthenium bis(chelate) compound 2 has the halide ligands and the coordinated azo nitrogen centers in an equatorial cis arrangement whereas two pyridyl groups (one of each abpy ligand) occupy the axial positions. The bond distances from the metal to the nitrogen donor centers are systematically smaller for the stronger π accepting azo functions than for the more basic but less π acidic pyridyl groups, a result which differs from that obtained for Mo(0) and Cu(I) complexes of abpy. All Ru—N distances are shorter in the neutral dichloro complex 2. The non-coordinated pyridyl rings of the potentially tetradentate abpy ligands are tilted into approximate s-cis/(NN-trans)/s-trans positions (dihedral angle ω ≈ 145°) as to minimize steric repulsion, however, they do not coordinate to the metal (dRu-N > 327 pm). While there are no significant intermolecular interactions, the observed conformation implies that considerable structural reorganization is necessary for the formation of oligonuclear complexes.
13
Chi, Kim N., Sebastien J. Hotte, Susan Ellard, Joel Roger Gingerich, Anthony Michael Joshua, Christian K. Kollmannsberger, Evan Y. Yu, and Martin Edwin Gleave. "A randomized phase II study of OGX-427 plus prednisone versus prednisone alone in patients with chemotherapy-naive metastatic castration-resistant prostate cancer." Journal of Clinical Oncology 30, no. 5_suppl (February 10, 2012): 121. http://dx.doi.org/10.1200/jco.2012.30.5_suppl.121.
Full textAbstract:
121 Background: Heat Shock Protein 27 (Hsp27) is a stress-activated, multi-functional chaperone protein highly expressed in cancer that regulates cell signaling and survival pathways implicated in cancer progression. In prostate cancer models, Hsp27 complexes with androgen receptor (AR) and enhances transactivation of AR-regulated genes. OGX-427 is a 2nd generation antisense that inhibits Hsp27 expression with in vitro and in vivo efficacy. Phase I studies have demonstrated tolerability and single agent activity. Methods: Patients (pts) with CRPC, no/minimal symptoms and any prior treatment other than chemotherapy were randomized 1:1 to receive prednisone (P) 5 mg PO BID or P with OGX-427 600 mg IV x 3 loading doses followed by 1000 mg IV weekly. Primary endpoint was the proportion of pts progression free (PSAWG 2 criteria) at 12 weeks. A 2-stage MinMax design (H0 = 5%, HA >20%, α=0.1, β=0.1) will enrol 32 pts total per arm and provide 70% power to detect the difference at a 0.10 1-sided significance. Secondary endpoints include PSA decline, measurable disease response, and circulating tumour cell (CTC) enumeration. Results: In the first 22 pts randomized (11 to OGX-427+P, 11 to P), baseline median age was 71 years (53-86), ECOG PS 0 or 1 in 64% and 36% of pts, median PSA 89 (6-606), metastases in bone/lymph nodes/liver or lung in 77%/64%/10%, 23% had prior treatment with P, and 91% had ≥5 CTC/7.5 ml (median 18/7.5 ml). Thus far, 82% of pts randomized to OGX-427+P have had a PSA decline (55% with ≥30% decline) and 18% a PSA increase; 40% of pts treated with P have had a PSA decline (20% with ≥30% decline), 10% no change and 50% with PSA increase. CTC conversion from ≥5 to <5/7.5 ml has occurred in 60% of pts randomized to OGX-427+P and 20% of pts treated with P alone. Grade 1-2 infusion reactions (e.g., chills, diarrhea, flushing) have occurred in 45% of pts receiving OGX-427+P and 1 pt developed hemolytic uremic syndrome after week 7 probably related to OGX. Conclusions: Preliminary data provide clinical support for the role of Hsp27 in AR signalling and as a therapeutic target for prostate cancer. Enrolment on this study continues. Funded by a grant from the Terry Fox Research Institute.
14
Nakayama, Yoshie, Shunji Takahashi, and Yoshinori Ito. "A phase II clinical trial of neoadjuvant therapy with zoledronic acid for operable breast cancer." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): e22027-e22027. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e22027.
Full textAbstract:
e22027 Background: The neoadjuvant use of bisphosphonates has been reported to increase rates of pathological response in patients with operable breast cancer. We conducted a study to determine whether treatment with zoledronic acid (ZOL) would improve outcomes in such patients when it was added to standard neoadjuvant chemotherapy (NAC). Methods: From April 2010 to June 2012, 30 patients with HER2-negative operable breast cancer were eligible for the study. The patients received NAC (4 cycles of CEF: intravenous epirubicin 100mg/m2, cyclophosphamide 500mg/m2, and fluorouracil 500mg/m2 on day 1, then 12 cycles of weekly paclitaxel 80mg/m2) with ZOL. The ZOL was administered after each cycle of NAC in a 4-mg dose by intravenous infusion every 3 to 4 weeks. The primary end point of the study was pathological response (pPR) rate. The secondary end points were pathological complete response (pCR) rate, adverse effects (AEs) and the changes in the numbers of disseminated tumor cells (DTC), circulating tumor cells (CTC), and circulating endothelial cells (CEC). We evaluated the efficacy and safety of ZOL in the NAC in patients by use of Fisher’s exact test. Results: 29 patients completed surgery after NAC, and pathological analyses and the numbers of CTC and CEC were assessed. Two patients had progressive disease during NAC, with brain metastasis and bone metastasis, respectively. The pPR rate, pCR rate, was 31.0%, 10.4%, respectively. CTC were detected in 3 patients at baseline, and CTC had changed to positive in one patient in D15 of the first cycle, then after NAC, all four patients changed to negative. CTC were negative in two patients at baseline, but after NAC, changed to positive. There were no factors associated with the change rates of CTC. CEC after NAC increased from baseline significantly (p<0.001). The increase rates of CEC were not significantly different among premenopausal and postmenopausal, PR and non-PR, and luminal and triple negative patients. There were no severe AEs associated with ZOL to discontinue treatment. Conclusions: These data suggested ZOL in combination with NAC might not have a significant direct anti-tumor effect. Change of CEC would be a biomarker for NAC, but further analyses would be necessary. Clinical trial information: 000003498.
15
Trzeciak, P., R. R. Starzynski, L. Rapala, S. Dabrowski, and A. M. Duszewska. "167 THE EFFECT OF ELEVATED TEMPERATURE ON THE HEAT SHOCK PROTEIN 70 (HSP70) EXPRESSION IN BOVINE CUMULUS–OOCYTE COMPLEXES AFTER IN VITRO MATURATION." Reproduction, Fertility and Development 27, no. 1 (2015): 174. http://dx.doi.org/10.1071/rdv27n1ab167.
Full textAbstract:
Elevated temperature during in vitro maturation negatively affects the oocyte's developmental competence, leading to disturbances in nuclear and cytoplasmic maturation. In previous studies, the role of cumulus granulosa cells (CGC) in cumulus-oocyte complexes' (COC) response to elevated temperature was underestimated. However, CGC play an essential role in folliculogenesis, supporting the oocyte's metabolism as well as meiotic progression. Thus, CGC may be engaged in COC response to heat shock. Heat shock protein 70 (HSP70) is the major protein engaged in cellular response to heat stress. The aim of this study was to determine the HSP70 expression in both CGC and oocytes in response to elevated temperature after COC in vitro maturation. COC were collected from bovine ovarian follicles (diameter 2–6 mm) from slaughtered cows and divided into two treatment groups: I (control) – COC were in vitro matured in control temperature (38.5°C); II (experimental) – COC were in vitro matured in elevated temperature (41°C). After in vitro maturation they were mechanically separated into CGC and oocyte. In vitro maturation was conducted in TCM199 25 mM HEPES medium supplemented with 10% FBS, 0.02 IU mL–1 NIH-pFSH, 1 μg mL–1 17β-oestradiol, 22 μg mL–1 Na-pyruvate and 10 μg mL–1 gentamicin, adjusted to pH 7.4 in 5% CO2. The HSP70 expression in CGC and oocytes was performed by real-time PCR and normalized to S18/H2A and S18 gene expression, respectively. In addition, the immunocytofluorescent analysis of HSP70 expression in CGC and oocyte's were performed. The HSP70 was stained using primary monoclonal mouse antibodies raised against bovine HSP70 and secondary antibody raised against mouse conjugated with Alexa 488. Nuclei were stained with Hoechst 33342. Slides were analysed under laser confocal microscope (FV-500, Olympus, Center Valley, PA, USA). The HSP70 expression in CGC was measured as total optical density of HSP70 and normalized to total optical density of nuclei. Statistical analyses were performed by Portable Statgraphics 5.0 Centurion. Mean values of HSP70 expression in CGC and oocytes in real-time PCR and immunofluorescent analysis in CGC were compared using Tukey's HSD test (α = 0.01). After in vitro maturation, the expression of HSP70 in CGC was higher (0.13 ± 0.052 a.u., n = 35) in experimental temperature compared to the control (0.058 ± 0.008 a.u., n = 35) (P < 0.01). In oocytes, HSP70 expression in experimental temperature (32.5 ± 5.2 a.u., n = 12) was similar to control (29.8 ± 5.6 a.u., n = 12). The immunofluorescent analysis confirmed data from real-time PCR analysis, indicating that the HSP70 expression in CGCs in experimental temperature was higher (0.46 ± 0.07 a.u., n = 10) compared to the control (0.12 ± 0.02 a.u., n = 10; P < 0.01). After COC in vitro maturation in stress conditions, the HSP70 expression was up-regulated in CGC, but not in oocytes. That may indicate that CGCs play the major role in COC response to elevated temperature; however, the analysis of other heat shock proteins expression in CGC and oocytes need to be conducted. Research was supported by 505-10-023300-K00169-99.
16
Mijušković, Zoran, Ljiljana Rackov, Janko Pejović, Sandra Živanović, Jelica Stojanović, and Zoran Kovačević. "Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis Patients." Journal of Medical Biochemistry 28, no. 3 (July 1, 2009): 166–71. http://dx.doi.org/10.2478/v10011-009-0016-9.
Full textAbstract:
Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis PatientsRheumatoid arthritis (RA) is predominantly an intraarticular inflammatory and autoimmune disease that involves different autoantibodies and effector mechanisms. The aim of the study was to determine the utility of Circulating Immune Complexes (CIC) and complement components (C3c, C4) as possible markers for the disease activity in laboratory diagnostics. In a cross-section study 59 patients, according to the clinical criteria, were categorized into two groups: group with moderate (MA, n=24), and group with severe activity (SA, n=35) of RA. The concentration of CIC, C3c and C4 in sera (S) and synovial fluids (SF) was examined by an immunonephelometric method in both groups and compared with values in the control group (n=15) of patients with lesions of the menisci. Obtained results showed that there was no statistical significance in the values of C3c and C4, in both biological fluids, among all tested groups. Significant differences were found in the levels of CIC in both fluids, while testing the parameters (× ± SD, IU/mL) in the sera of groups with SA and MA of RA: 7.43 ± 13.40; 3.01 ± 2.92 (p<0.05) and SF: 13.47 ± 21.1, 5.33 ± 7.53 (p<0.001), respectively. These differences were higher between the group with SA and CG. Results for the concentrations of CIC were significantly higher in SF compared to sera: in the RA group with SA by 77% and group with MA by about 82%. These data could provide a confirmation of the hypothesis about local, intraarticular autoantibodies and subsequent CIC production. It can be concluded that the examination of CIC concentration in serum, and where it is possible in SF, is a useful marker of disease activity in RA patients, in contrast to the tested components of the complement. This statement does not exclude their consumption within immune effector mechanisms, but elicits the possibility that lower molecular fragments (C3d, C4d), as well as the novel activation products, could be better disease activity markers in RA patients.
17
Chang, Po-Hao, Min-Che Chen, Ya-Ping Tsai, Grace Y. T. Tan, Pang-Hung Hsu, Yung-Ming Jeng, Yi-Fang Tsai, Muh-Hwa Yang, and Wendy W. Hwang-Verslues. "Interplay between desmoglein2 and hypoxia controls metastasis in breast cancer." Proceedings of the National Academy of Sciences 118, no. 3 (January 11, 2021): e2014408118. http://dx.doi.org/10.1073/pnas.2014408118.
Full textAbstract:
Metastasis is the major cause of cancer death. An increased level of circulating tumor cells (CTCs), metastatic cancer cells that have intravasated into the circulatory system, is particularly associated with colonization of distant organs and poor prognosis. However, the key factors required for tumor cell dissemination and colonization remain elusive. We found that high expression of desmoglein2 (DSG2), a component of desmosome-mediated intercellular adhesion complexes, promoted tumor growth, increased the prevalence of CTC clusters, and facilitated distant organ colonization. The dynamic regulation of DSG2 by hypoxia was key to this process, as down-regulation of DSG2 in hypoxic regions of primary tumors led to elevated epithelial−mesenchymal transition (EMT) gene expression, allowing cells to detach from the primary tumor and undergo intravasation. Subsequent derepression of DSG2 after intravasation and release of hypoxic stress was associated with an increased ability to colonize distant organs. This dynamic regulation of DSG2 was mediated by Hypoxia-Induced Factor1α (HIF1α). In contrast to its more widely observed function to promote expression of hypoxia-inducible genes, HIF1α repressed DSG2 by recruitment of the polycomb repressive complex 2 components, EZH2 and SUZ12, to the DSG2 promoter in hypoxic cells. Consistent with our experimental data, DSG2 expression level correlated with poor prognosis and recurrence risk in breast cancer patients. Together, these results demonstrated the importance of DSG2 expression in metastasis and revealed a mechanism by which hypoxia drives metastasis.
18
Andrew, Rhiann E., Lucero González-Sebastián, and Adrian B. Chaplin. "NHC-based pincer ligands: carbenes with a bite." Dalton Transactions 45, no. 4 (2016): 1299–305. http://dx.doi.org/10.1039/c5dt04429d.
Full textAbstract:
In this frontier article we overview the emergence and scope of NHC-based CCC and CNC pincer systems, i.e. complexes containing mer-tridentate ligands bearing two NHC donor groups, comment on their effectiveness in applications, and highlight areas for future development and exploitation.
19
Abeydeera, Lalantha R., Hiroaki Funahashi, Nam-Hyung Kim, and Billy N. Day. "Chlortetracycline fluorescence patterns and in vitro fertilisation of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations." Zygote 5, no. 2 (May 1997): 117–25. http://dx.doi.org/10.1017/s0967199400003798.
Full textAbstract:
SummaryPorcine oocyte-cumulus complexes were cultured in bovine serum albumin(BSA)-free North Carolina State University (NCSU)23 medium containing porcine follicular fluid (10%), cysteine (0.1 mg/ml)and hormonal supplements (eCG and hCG: 10IU/ml each) for 22h. They were then cultured in the same medium but without hormonal supplements for an additional 22h. After culture, cumulus cells were removed and oocytes were co-incubated with frozen-thawed ejaculated boar spermatozoa in tissue culture medium (TCM)199 containing caffeine (5mM), fetal calf serum (FCS; 10%) and varying concentrations (26–56 mM)of NaHCO3 for 9h(experiment 1). In experiment 2, chlortetracycline (CTC) was used to assess the functional state of spermatozoa incubated under different NaHCO3 concentrations. Experiment 3 examined the effect of FCS (1% and 10%)and NaHCO3 (26 and 46 mM) on fertilisation parameters. Compared with 26 mM, penetration rate was significantly higher (p<0.05)at 36–56mM NaHCO3. Polyspermy showed a similar pattern although no difference was observed between 26 and 36 mM. At 46mM NaHCO3, the mean number of spermatozoa (MNS) penetrated per oocyte increased significantly (p< 0.05). A significantly higher proportion of spermatozoa were capacitated and acrosome reacted at 46 and 56mM NaHCO3, respectively. The fertilisation medium containing 46mM NaHCO3 and 1% FCS showed a higher penetration rate (84%)with a relatively low incidence of polyspermy (39%). The results indicate that NaHCO3 stimulates capacitation and/or the acrosome reaction of boar spermatozoa in a dose-dependent manner and thus affects fertilisation parameters.
20
Truong, Thu Ha, Elizabeth A. Benner, Kyla M. Hagen, Nuri A. Temiz, Carlos Perez Kerkvliet, Ying Wang, Emilio Cortes-Sanchez, et al. "Steroid Receptor Co-Activators Regulate Metabolic Kinases to Drive Therapy Resistant ER+ Breast Cancer." Journal of the Endocrine Society 5, Supplement_1 (May 1, 2021): A1031—A1032. http://dx.doi.org/10.1210/jendso/bvab048.2111.
Full textAbstract:
Abstract Recurrence of metastatic breast cancer stemming from acquired endocrine and chemotherapy resistance remains a health burden for women with luminal (ER+) breast cancer. Disseminated ER+ tumor cells can remain viable but quiescent for years to decades. Contributing factors to metastatic spread include the maintenance and expansion of breast cancer stem cells (CSCs). Breast CSCs are poorly proliferative and frequently exist as a minority population in therapy resistant tumors. Our objective is to define novel signaling pathways that govern therapy resistance in ER+ breast cancer. In this study, we show that cytoplasmic complexes composed of steroid receptor (SR) co-activators, PELP1 and SRC-3, modulate breast CSC expansion through upregulation of the HIF-activated metabolic target genes PFKFB3 and PFKFB4. Seahorse metabolic assays demonstrated that cytoplasmic PELP1 influences cellular metabolism by increasing both glycolysis and mitochondrial respiration. PELP1 interacts with PFKFB3 and PFKFB4 proteins, and inhibition of PFKFB3 and PFKFB4 kinase activity blocks PELP1-induced tumorspheres and protein-protein interactions with SRC-3. PFKFB4 knockdown inhibited in vivo emergence of circulating tumor cell (CTC) populations in ER+ mammary intraductal (MIND) xenografts. Application of PFKFB inhibitors in combination with ER targeted therapies blocked tumorsphere formation in multiple models of advanced breast cancer, including tamoxifen (TamR) and paclitaxel (TaxR) resistant models and ER+ patient-derived organoids (PDxO). Together, our data suggest that PELP1, SRC-3, and PFKFBs cooperate to drive ER+ tumor cells that include CSCs and CTCs. Identifying non-ER pharmacological targets offers a useful approach to blocking metastatic escape from standard of care ER/estrogen (E2)-targeted strategies to overcome endocrine and chemotherapy resistance in ER+ breast cancer.
21
Moreira, Rafaele R., Natalia A. Peres, and Louise L. May De Mio. "Colletotrichum acutatum and C. gloeosporioides Species Complexes Associated with Apple in Brazil." Plant Disease 103, no. 2 (February 2019): 268–75. http://dx.doi.org/10.1094/pdis-07-18-1187-re.
Full textAbstract:
Glomerella leaf spot (GLS) is an apple disease that concerns growers due to the increases in severity over the years and the difficulties in control. Species within the Colletotrichum acutatum and C. gloeosporioides species complexes cause GLS, but the proportion of species within each complex in Brazilian apple orchards is not known. The objectives of this study were to identify isolates of Colletotrichum causing GLS on apple orchards in the main Brazilian producing regions to the species level. Two hundred and seven isolates were obtained in orchards in São Paulo (SP), Parana (PR), Santa Catarina (SC), and Rio Grande do Sul (RS) states. Genomic DNA was extracted, and the ITS, GAPDH, CHS-1, and TUB2 genes were amplified and sequenced. The phylogenetic trees were generated using a concatenated alignment. One hundred and fourteen isolates were identified as belonging to the C. acutatum species complex (Cac) and 93 to the C. gloeosporioides species complex (Cgc). Five phylogenetic species were identified: C. melonis (1.9%), C. nymphaeae (47.4%), C. paranaense (2.4%), C. limetticola (3.4%), and C. fructicola (44.9%). In SC, Cgc predominates, but in the states of SP, PR, and RS, Cac was predominant. This is the first report of C. limetticola from apple.
22
Gründemann, Stephan, Martin Albrecht, Jennifer A. Loch, Jack W. Faller, and Robert H. Crabtree. "Tridentate Carbene CCC and CNC Pincer Palladium(II) Complexes: Structure, Fluxionality, and Catalytic Activity." Organometallics 20, no. 25 (December 2001): 5485–88. http://dx.doi.org/10.1021/om010631h.
Full text
23
Paraan, Mohammadreza, Joshua Mendez, Savanna Sharum, Danielle Kurtin, Huan He, and Scott M. Stagg. "The structures of natively assembled clathrin-coated vesicles." Science Advances 6, no. 30 (July 2020): eaba8397. http://dx.doi.org/10.1126/sciadv.aba8397.
Full textAbstract:
Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.
24
Klyushkin, I. V., E. E. Krasnoshchekova, K. T. Valeeva, and M. N. Nasrullaev. "Assessment of the immunological status in patients of a surgical hospital." Kazan medical journal 74, no. 3 (June 15, 1993): 218–19. http://dx.doi.org/10.17816/kazmj64704.
Full textAbstract:
In patients with a surgical profile, the humoral link of the immune system was studied for the production of 3 classes of immunoglobulins (IgA, IgM, IgG), the formation of circulating immune complexes (CIC), bactericidal activity of blood serum (BASK) in comparison with the clinical picture of the disease. Serum immunoglobulins were determined according to Mancini, CEC by the conventional method according to D. K. Novikov (1987), BASK by the method of V. I. Nikitenko and O. V. Bukharin.
25
Garcia, Jorge A., Paul Elson, Matthew M. Cooney, Allison Janine Tyler, Victoria Rezash, Ernest C. Borden, and Robert Dreicer. "Inhibition of the IGF pathway in metastatic castrate resistant prostate cancer (CRPC): Results from a phase II study of OSI-906 (linsitinib)." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): e16022-e16022. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e16022.
Full textAbstract:
e16022 Background: AR ligand independent pathways are responsible for the development of CRPC. IGF-1R and its ligands (IGF-1/IGF-2) play a key role in regulating growth, resistance to apoptosis, and invasion in PCa. IGF-1R is overexpressed in PCa and mediates resistance to ADT. Linsitinib is a small molecule potent dual inhibitor of IGF-1R and insulin receptor TK activity. To determine the activity of linsitinib in men with CRPC a Simon two-stage phase II study was conducted. Methods: Men with asymptomatic or mildly symptomatic Met CRPC were eligible. ECOG 0-1, adequate organ function, and no prior chemotherapy for CRPC were required. Pts received linsitinib at 150mg orally twice daily on a 28-day cycle. Endpoints included PSA response at 12 wks, safety, RECIST-defined response, time to PSA progression, and OS. Correlative studies explored the impact of linsitinib on AR signaling (androstenedione, DHEA, DHEA-sulfate, p-IGF-1R, p-IR), markers of PD (TGF-b1, IL-6, TNF-a, MCP-1), and CTC and CECs. Results: Eighteen pts were enrolled and completed 12 wks of therapy. Median age 68 (55-78); Median pre-treatment PSA was 55.23 (range 2.46-277.60); 41% of pts had bone-only disease, 29% soft-tissue only and 29% had both. Although transient PSA declines were noted (8/18), seventeen pts discontinued therapy sec PSA progression. RECIST-defined SD was observed in 8 pts. Pts received a median of 3 cycles of therapy (range 2-6+). Most common G1/2 AEs included AST/ALT changes observed in 41 and 18% of patients respectively, fatigue (59%); prolonged QT interval (35%) and nausea/vomiting (35%). No significant change in the number of CTC and CEC counts was observed. An association between pre-treatment PSA and pre-treatment CTC was observed (Spearman r=0.49, p=.04). No correlation between CTC changes and PSA progression was observed .Evaluation of other molecular markers of PD is underway. Conclusions: Treatment with linsitinib is safe however no PCa activity was seen. No changes in CTC/CECs were noted. Markers of PD might help elucidate potential mechanisms of escape to this alternative pathway in CRPC. Linsitinib plus an AA might provide synergistic activity in this setting. Clinical trial information: NCT01533246.
26
Zhang, Jingsong, Jesse Mocha, and Ghassan El-Haddad. "Changes in prostate cancer circulating tumor cell (CTC) numeration and gamma H2AX positivity before and after treatment with radium 223 for metastatic castrate resistant prostate cancer." Journal of Clinical Oncology 36, no. 6_suppl (February 20, 2018): 211. http://dx.doi.org/10.1200/jco.2018.36.6_suppl.211.
Full textAbstract:
211 Background: As an alpha-particle emitting radiopharmaceutical, R is indicated for symptomatic mCRPC patients with bone predominant metastases and no visceral metastases. The current R administration dose was calculated based on body weight and decay factor. This may not be an ideal dose to individual patient (pt) with different body mass and different burdens of bone metastases. We hypothesize that changes in CTC # and the DNA damage marker, γH2AX, could serve as readout for on target effects of R and conducted a pilot prospective biomarker study. Methods: Pts who received R as standard of care for mCRPC and had a baseline CTC # of ≥ 2 were eligible. CTC samples were collected prior to and between 24 and 96 hours post dose 1, 3 and 6 of R. CTC # and γH2AX positivity were performed by Janssen Diagnostics Lab. Results: 10 pts were enrolled and all had > 15 bone metastases at enrollment. 7/10 pts had prior Taxane-based chemotherapy. The median CTC # and γH2AX positivity prior to R were 21 (range: 3-250), and 33% (range: 3% to 80%) respectively. 8 pts completed all 6 doses of R; 2 pts completed 2 doses then elected to hospice due to rapid clinical decline. Among pts completed R, 4 (50%) had bone scan progression within 1 month. No decline in ALKP, PSA, CTC # or increase in gamma H2AX positivity were seen during treatment with R in these 4 pts. 2 pts had CTC # conversion to 0 after dose 3 R at week 9. Both pts had ALKP decline, but no PSA decline during treatment. 1 of these 2 pts had 100% positivity for gamma H2AX after dose 6 R and had prolonged post R PSA response with stable bone scans at last follow up 24 months after the first dose of R. Increases in CTCs’ γH2AX positivity, and > 50% decline of ALKP were observed in 2 pts with germline deleterious mutations in BRCA2 and PALB2. Both pts, however, had a rapid surge in CTC # and PSA after dose 3. Despite stable bone scans, both proceeded with chemotherapy within 2 months after completing dose 6 R. Conclusions: Increase in CTC # and no increase in γH2AX positivity were associated with lack of response to R. The lack of increase in the DNA damage marker, γH2AX, in pts without declines in ALKP, PSA, and CTC # suggests their R doses may need to be increased. Clinical trial information: NCT02981797.
27
Boysen, Marta, Laura Schlicksupp, Ingeborg Dreher, Ralf Loebbert, and Mario Richter. "SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix." Journal of Immunology Research 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/9096059.
Full textAbstract:
Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles’ heel, it is their potential immunogenicity. To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions inMacaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC).
28
Mueller, V., S. Riethdorf, S. Loibl, M. Komor, J. Houber, I. Schrader, U. Conrad, M. Untch, G. vMinckwitz, and K. Pantel. "Prospective monitoring of circulating tumor cells in breast cancer patients treated with primary systemic therapy—A translational project of the German Breast Group study GeparQuattro." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 21085. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.21085.
Full textAbstract:
21085 Background: The impact of circulating tumor cells (CTC) in patients with primary breast cancer is still unclear. Primary systemic treatment (PST) allows the assessment of therapeutic efficacy in breast cancer patients without long follow-up periods. Here we present first results on the presence of CTC in peripheral blood of patients enrolled in the “GeparQuattro” study. Methods: This study incorporates three different chemotherapy approaches and additional Trastuzumab (Herceptin®) treatment for patients with HER-2/neu-positive tumors. Recruitment finished in November 2006 and not all patients have completed therapy yet. We used the CellSearch™ system to evaluate CTC before PST from 245 patients and after PST from 67 patients. CTC from 45 samples were also examined for HER-2/neu expression by immunocytochemistry in the CellSearch™ system. Results: Before PST we detected CTC in 54/245 patients (22%). CTC numbers in 7.5 mL blood ranged between 1 and 200 (mean 6.5). In 8 CTC-positive samples (3.3%) = 5 CTC were found. Her-2/neu-positive CTC were observed in 25/45 cases (55.6%). CTC could be detected in 7/67 patients (10.4%) after PST (1 or 2 CTC). Before and after PST blood was analyzed from 43 patients, 27 of them were CTC-negative at both time points. Ten initially CTC-positive cases were CTC-negative after PST whereas 6 cases were detected CTC-positive after PST although no CTC could be found before PST. Conclusions: With the CellSearch™ system CTC can be detected in non-metastatic breast cancer patients at primary diagnosis and also after PST. To our knowledge, this is the largest study evaluating the presence of CTC in this context. With the availability of response information from more patients, it will be possible to examine the correlation between the incidence of CTC and response as well as kinetics of HER-2/neu expression during Trastuzumab treatment. [Table: see text]
29
Ikonomov, V., W. Samtleben, B. Schmidt, M. Blumenstein, and H. J. Gurland. "Adsorption Profile of Commercially Available Adsorbents: An in Vitro Evaluation." International Journal of Artificial Organs 15, no. 5 (May 1992): 312–19. http://dx.doi.org/10.1177/039139889201500511.
Full textAbstract:
Adsorbents from four commercially available devices, Protein A-Sepharose (Immunosorba Protein A-62,5; Excorim KB, Lund Sweden), Tryptophan-PVA (Immusorba TR-350; Asahi Medical Co., Tokyo, Japan), Phenylalanine-PVA (Immusorba PH-350; Asahi Medical Co., Tokyo, Japan), and Dextran sulfate (Liposorber LA-15; Kanegafuchi Chemical Co. Ltd, Osaka, Japan) were tested under optimal in vitro conditions to determine their adsorption capability for several plasma constituents which are usually the target of plasma therapy. The parameters of interest were: double stranded DNA-antibodies (anti-dsDNA), antiglomerular basement membrane antibodies (anti-GBM), antiacetylcholin receptor antibodies (AChRAb), circulating immune complexes (CIC), rheumatoid factor (RF), IgA, IgG, IgM, IgE, C3c, C4, LDL-cholesterol, total cholesterol, erythropoietin (EPO) and β2-microglobulin (β2M). The IgG auto antibodies, CIC and RF can be removed by Protein A-Sepharose, Try-PVA and Phe-PVA. IgG is best adsorbed by Protein A-Sepharose, while IgE can be removed effciently by Try-PVA. Dextran sulfate is without doubt the best adsorbent for LDL-cholesterol. All four adsorbents bind also complement components C3c and C4. No significant adsorption was found for EPO and β2M. The four devices exhibit a quite different adsorption profile which can be used as a guide for the optimal selection of an adsorption column in clinical apheresis.
30
Garcia, Jorge A., Paul Elson, Matthew M. Cooney, Allison Janine Tyler, Victoria Rezash, Ernest C. Borden, and Robert Dreicer. "A phase II study of linsitinib (OSI-906) in patients with asymptomatic or mildly symptomatic (non-opioid requiring) metastatic castrate resistant prostate cancer (CRPC)." Journal of Clinical Oncology 31, no. 6_suppl (February 20, 2013): 197. http://dx.doi.org/10.1200/jco.2013.31.6_suppl.197.
Full textAbstract:
197 Background: Novel agents capable of blocking AR ligand independent pathways responsible for the development of CRPC are needed. IGF-1R and its ligands (IGF-1/IGF-2) play a key role in regulating growth, resistance to apoptosis, and invasion in PCa. IGF-1R is overexpressed in PCa and mediates resistance to ADT. OSI-906 is a small molecule potent dual inhibitor of IGF-1R and insulin receptor TK activity. To determine the activity of OSI-906 in men with CRPC a Simon two-staged phase II study was conducted. Methods: Men with asymptomatic or mildly symptomatic Met CRPC were eligible. ECOG 0-1, adequate organ function, and no prior chemotherapy for CRPC were required. Pts received OSI-906 at 150mg orally twice daily on a 28-day cycle. Endpoints included PSA response at 12 wks, safety, RECIST-defined response, time to PSA progression, and OS. Correlative studies explored the impact of OSI-906 on AR signaling (androstenedione, DHEA, DHEA-sulfate, p-IGF-1R, p-IR), markers of PD (TGF-b1, IL-6, TNF-a, MCP-1), and CTC and CECs. Results: To date 18 pts have enrolled and completed 12 wks of therapy. Median age 68 (55-78); Median pre-treatment PSA was 55.23 (range 2.46-277.60); 41% of pts had bone-only disease, 29% soft-tissue only and 29% had both. Although transient PSA declines were noted, 17/18 pts discontinued therapy sec PSA progression. RECIST-defined SD was observed in 8 pts. Pts received a median of 3 cycles of therapy (range 2-6+). Most common G1/2 AEs included AST/ALT changes observed in 41 and 18% of patients respectively, fatigue (59%); prolong QT interval (35%) and nausea/vomiting (35%). No significant change in the number of CTC and CEC counts was observed. An association between pre-treatment PSA and pre-treatment CTC was observed (Spearman r=0.49, p=.04). No correlation between CTC changes and PSA progression was observed .Evaluation of other molecular markers of PD is underway. Conclusions: Treatment with OSI-906 is safe. No PSA responses or changes in CTC/CECs were noted. Markers of PD might help elucidate potential mechanisms of escape to this alternative pathway in CRPC. OSI-906 plus an AA might provide synergistic activity in this setting. Clinical trial information: NCT01533246.
31
Wang, Ben, Jun Ye, and Jian Xiong. "The Effect of pH on the Fluorescence Properties of Thermally Stable CMC/Eu Complexes." Advanced Materials Research 1061-1062 (December 2014): 141–44. http://dx.doi.org/10.4028/www.scientific.net/amr.1061-1062.141.
Full textAbstract:
CMC/Eu complexes were synthesized in different pH values by reacting with Eu3+ and CMC, a kind of biodegradable polymer. FT-IR results confirmed that, on CMC chains, carboxyl groups and the oxygen atom of unsubstituted hydroxyl groups and ether bonds were all involved in the reaction with Eu3+. TG analysis showed that CMC/Eu complexes were thermally stable in a wide temperature range from 30 to 300°C. The emission intensity decreased when the pH value increased from 7.0 to 12.0. However, at pH=12, because that the extent of reaction of Eu3+ and CMC decreased and CMC degraded, the complexes had a weak fluorescence intensity.
32
MaGee, Karen D. M., Guy Travers, Brian W. Skelton, Massimilliano Massi, Alan D. Payne, and David H. Brown. "Synthesis, Solid-state Structures, Solution Behaviour and Catalysis Studies of Nickel Complexes of Bis(benzimidazolin-2-ylidene)pyridine Pincer Ligands." Australian Journal of Chemistry 65, no. 7 (2012): 823. http://dx.doi.org/10.1071/ch12044.
Full textAbstract:
N-Heterocyclic carbene–nickel complexes with five- and four-coordinate geometries [(CNC)NiBr2] and [(CNC)NiBr]X (X = PF6 or BPh4) have been prepared with the pincer ligands 2,6-bis(N-octylbenzimidazolin-2-ylidene)pyridine and 2,6-bis(N-butyl-5,6-dimethoxybenzimidazolin-2-ylidene)pyridine. The addition of the n-octyl substituent significantly extends the solubility of the complexes and has allowed UV-vis solution studies of the complexes in dichloromethane and methanol. The four- and five-coordinate species exist in equilibrium in solution and this equilibrium has been explored by UV-vis studies. The complexes have also been characterized by NMR studies, and single crystal X-ray diffraction studies have been performed on [(CNC)NiBr2] (where CNC = 2,6-bis(N-octylbenzimidazolin-2-ylidene)pyridine) and [(CNC)NiBr]BPh4 (where CNC = 2,6-bis(N-butyl-5,6-dimethoxybenzimidazolin-2-ylidene)pyridine).
33
Corral, Ricardo, Héctor Freilij, and Saúl Grinstein. "Specific circulating immune complexes in acute chagas' disease." Revista do Instituto de Medicina Tropical de São Paulo 29, no. 1 (February 1987): 26–32. http://dx.doi.org/10.1590/s0036-46651987000100004.
Full textAbstract:
The presence of circulating immune complexes formed by IgM and IgG (CIC-IgM and CIC-IgG) was investigated, using antigen-specific enzyme-immunoassays (ELISA), in 30 patients with acute Chagas' disease who showed parasitemia and inoculation chagoma. Control population consisted of patients with chronic T. cruzi infection (30), acute toxoplasmosis 10), leishmaniasis (8), rheumatoid arthritis (3) and healthy individuals with negative serology for Chagas* disease (30). Acute chagasic patients were 100% CIC-IgG and 96.66% CIC-IgM positive whereas immunofluorescence tests yielded 90% and 86.66% of positivity for specific IgG and IgM antibodies, respectively. Chronic patients were 68% CIC-IgG and 0% CIC-IgM positive. The 30 negative and the 21 cross-reaction controls proved negative for ELISA (CIC-IgM and CIC-IgG). The high sensitivity of ELISA assays would allow early immunologic diagnosis, as well as prompt treatment, of acute T. cruzi infection, thus eliminating the problem of the false-positive and false-negative results which affects traditional methods for detection of circulating antibodies.
34
DeCastro, Guarionex Joel, Wilson Sui, Jamie S. Pak, Corinne T. Abate-Shen, Shing Mirn Lee, Christopher B. Anderson, Dara Holder, and James M. McKiernan. "A phase I trial for the use of intravesical cabazitaxel, gemcitabine, and cisplatin (CGC) in the treatment of BCG-refractory nonmuscle invasive urothelial carcinoma of the bladder." Journal of Clinical Oncology 35, no. 6_suppl (February 20, 2017): 313. http://dx.doi.org/10.1200/jco.2017.35.6_suppl.313.
Full textAbstract:
313 Background: Salvage intravesical chemotherapy has shown benefit in patients with high-risk non-muscle invasive bladder cancer who fail first line therapy. Our preclinical murine intravesical trial showed a combination therapy was superior to single-agents. Our objective was to investigate the safety of intravesical triple agent salvage chemotherapy consisting of cabazitaxel, gemcitabine and cisplatin (CGC). Methods: Patients with BCG refractory or recurrent high-risk non-muscle invasive bladder cancer who refused radical cystectomy were enrolled. All patients underwent a pre-treatment transurethral resection of bladder tumor and then received a 6-week regimen. All patients received the same dose of gemcitabine (2000mg) while the dose of cisplatin and cabazitaxel were escalated as shown in table 1. Toxicity was categorized according to CTC for Adverse Events v4 and included hematuria, dysuria, bladder spasm, urinary retention or frequency. A complete response (CR) was defined as a negative random bladder biopsy and negative cytology six weeks after treatment. Results: Median age of the 9 patients was 74 years (table 1) and the median number of prior intravesical therapies was 4 (range 2-5). All patients completed 6 weeks of induction CGC. Any local toxicity was found in 7 patients with 5 experiencing at least 1 grade 1 toxicity and 4 experiencing at least 1 grade 2 toxicity. Seven of eight patients were complete responders and initiated maintenance therapy. Conclusions: CGC appears to be a well-tolerated intravesical salvage chemotherapy regimen for the treatment of BCG-refractory NMIBC. Clinical trial information: NCT02202772. [Table: see text]
35
Berland, Kevin, Justin B. Renaud, and Paul M. Mayer. "Utilizing ion mobility and tandem mass spectrometry to evaluate the structure and behaviour of multimeric cyclodextrin complexes." Canadian Journal of Chemistry 93, no. 12 (December 2015): 1313–19. http://dx.doi.org/10.1139/cjc-2014-0419.
Full textAbstract:
Characterizing noncovalent complexes of molecular dimers and higher complexes using tandem mass spectrometry (MS/MS) can be hindered due to spectral overlap in both the MS and the MS/MS. We investigated the structures and dissociation energetics of multimeric β-cyclodextrin (β-CD) complexes alone or with substrates using combinations of ion mobility spectrometry (IMS), MS/MS, and Rice–Ramsperger–Kassel–Marcus (RRKM) unimolecular rate modelling. The doubly charged β-CD dimers ([(β-CD)2 – 2H+]2−) dissociate to two [β-CD – H+]− ions with the same m/z. IMS was used to separate source generated [(β-CD)2 – 2H+]2− from [β-CD – H+]− and the extent of [(β-CD)2 – 2H+]2− dissociation versus collision energy was determined by modelling changes in the ion’s isotopic profile. The RRKM derived critical energy (E0) for dissociation of [(β-CD)2 – H+]− and [(β-CD)2 – 2H+]2− were 1.85 ± 0.11 eV and 1.79 ± 0.09 eV, respectively, corresponding to a slight decrease in complex stability due to increased charge–charge repulsion in the dianion. This approach was extended to include dimeric complexes complexed to 4,4′-(propane-1,3-diyl) dibenzoic acid (PDDA) and ibuprofen (Ibu).
36
Wiest, D. L., K. P. Kearse, E. W. Shores, and A. Singer. "Developmentally regulated expression of CD3 components independent of clonotypic T cell antigen receptor complexes on immature thymocytes." Journal of Experimental Medicine 180, no. 4 (October 1, 1994): 1375–82. http://dx.doi.org/10.1084/jem.180.4.1375.
Full textAbstract:
CD3 signal transducing proteins are thought to be expressed on the surface of T cells only as part of clonotypic T cell receptor (TCR) complexes. Contrary to this paradigm, the present study describes surface expression of CD3 proteins independently of clonotypic TCR complexes, but only on immature thymocytes. Such novel clonotype-independent CD3 (CIC) complexes are composed primarily of CD3 gamma epsilon and secondarily of CD3 delta epsilon heterodimers that are independent of one another and are expressed on the cell surface in association with an unknown 90-100 kD protein termed CD3-associated protein (CD3AP). CIC complexes are expressed in normal mice on early thymocytes through the CD4+CD8+ stage of development, but not on mature peripheral T cells. Furthermore, CIC complexes are expressed by both TCR- severe combined immunodeficiency (SCID) thymocytes and thymoma cell lines, in the absence of any clonotypic chains. The isolation and biochemical characterization of surface CIC complexes provides a structural basis for the signaling effects of anti-CD3 epsilon antibody treatment in early thymocyte development.
37
Dębowski, Mateusz, Zuzanna Szymańska, Jacek Z. Kubiak, and Mirosław Lachowicz. "Mathematical Model Explaining the Role of CDC6 in the Diauxic Growth of CDK1 Activity during the M-Phase of the Cell Cycle." Cells 8, no. 12 (November 28, 2019): 1537. http://dx.doi.org/10.3390/cells8121537.
Full textAbstract:
In this paper we propose a role for the CDC 6 protein in the entry of cells into mitosis. This has not been considered in the literature so far. Recent experiments suggest that CDC 6 , upon entry into mitosis, inhibits the appearance of active CDK 1 and cyclin B complexes. This paper proposes a mathematical model which incorporates the dynamics of kinase CDK 1 , its regulatory protein cyclin B, the regulatory phosphatase CDC 25 and the inhibitor CDC 6 known to be involved in the regulation of active CDK 1 and cyclin B complexes. The experimental data lead us to formulate a new hypothesis that CDC 6 slows down the activation of inactive complexes of CDK 1 and cyclin B upon mitotic entry. Our mathematical model, based on mass action kinetics, provides a possible explanation for the experimental data. We claim that the dynamics of active complexes CDK 1 and cyclin B have a similar nature to diauxic dynamics introduced by Monod in 1949. In mathematical terms we state it as the existence of more than one inflection point of the curve defining the dynamics of the complexes.
38
Meropol, N. J., S. J. Cohen, N. Iannotti, B. H. Saidman, K. D. Sabbath, M. C. Miller, G. V. Doyle, H. Tissing, L. W. M. M. Terstappen, and C. J. A. Punt. "Circulating tumor cells (CTC) predict progression free (PFS) and overall survival (OS) in patients with metastatic colorectal cancer." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 4010. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.4010.
Full textAbstract:
4010 Background: Treatment options for patients with metastatic colorectal cancer (mCRC) have recently expanded. However, the introduction of new regimens has highlighted the need for biomarkers to guide and monitor therapy. This study investigates the hypothesis that CTC levels can predict clinical outcomes in patients with mCRC. Methods: In a prospective multi-center study, 7.5 mL of blood from 456 patients with mCRC were tested for CTC using immunomagnetic separation before starting 1st, 2nd or 3rd line therapy and at subsequent radiographic follow-up timepoints. Response on imaging was determined by participating centers. Results: Patients were stratified into unfavorable and favorable prognostic groups based on levels of =3 or <3 CTC per 7.5mL, respectively. Median PFS, OS and significance between the two groups (log rank test) at different timepoints after the initiation of therapy is indicated in the table . The OS of patients converting to or maintaining unfavorable CTC at follow up timepoints was worse than for patients maintaining favorable CTC (12.6, 7.0 vs. 21.1 months, respectively, p<0.0001). In multivariate analyses, which included age, ECOG performance status, and the line and type of therapy, CTC remained the most significant independent predictor of outcome. Conclusions: CTC levels before treatment and at subsequent timepoints are an independent predictor of PFS and OS in patients with mCRC. CTC levels of =3 per 7.5mL of blood after initiation of therapy are highly predictive of shorter PFS and OS. A large randomized phase III study to confirm these findings in mCRC patients receiving first line therapy recently completed accrual (DCCG CAIRO- 2). [Table: see text] No significant financial relationships to disclose.
39
Papenfuhs, Bernd, Thomas Dirnberger, and Helmut Werner. "Preparation of hydrido(vinyl)iridium(III) complexes from functionalized olefins by CH activation." Canadian Journal of Chemistry 84, no. 2 (February 1, 2006): 205–13. http://dx.doi.org/10.1139/v05-234.
Full textAbstract:
The four-coordinate iridium(I) precursor trans-[IrCl(N2)(PPh3)2] (1) reacts with functionalized olefins RCH=C(R′)C(O)R′′ by displacement of the dinitrogen ligand and oxidative addition of the CH bond to the metal center to give six-coordinate hydrido(vinyl)iridium(III) complexes [IrH(Cl){κ2(C,O)-C(R)=C(R′)C(R′′)=O}(PPh3)2] (212) in good to excellent yields. The reaction of [IrH(Cl){κ2(C,O)-CH=CHC(Me)=O}(PPh3)2] (2) with AgClO4 affords the cationic compound [IrH{κ2(C,O)-CH=CHC(Me)=O}(PPh3)2]ClO4 (15), which in solution equilibrates with the uncharged isomers [IrH(OClO3){κ2(C,O)-CH=CHC(Me)=O}(PPh3)2] (15a and 15b). In contrast, five-coordinate [IrH{κ2(C,O)-CH=CHC(Et)=O}(PPh3)2]ClO4 (16), prepared from 3 and AgClO4, is stable and undergoes addition reactions with CO, PPh3, C2H4, CH3C≡CH, MeCN, and PhCN to give the six-coordinate complexes [IrH(L){κ2(C,O)-CH=CHC(Et)=O}(PPh3)2]ClO4 (1722). The neutral hydrido(thiolato) compound [IrH(SPh){κ2(C,O)-CH=CHC(Et)=O}(PPh3)2] (23) was obtained on treatment of 21 with NaSPh. Key words: iridium, CH activation, hydrido complexes, vinyl complexes, phosphine complexes.
40
Liu, M. C., P. Shields, C. Isaacs, R. Warren, P. Cohen, M. Wilkinson, Y. Zhang, R. Shen, K. Luyegu, and A. L. Gallagher. "Circulating tumor cells (CTC): Assessment of treatment efficacy in metastatic breast cancer (MBC)." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 10535. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.10535.
Full textAbstract:
10535 Background: Preliminary data in MBC suggest that ≥5 CTC/7.5 mL blood is associated with worse progression free survival (PFS) and overall survival (OS), and that the persistence of ≥5 CTC/7.5 mL blood after the initiation of therapy predicts for treatment failure (NEJM 2004. 351:781.). We are conducting a prospective clinical research study to validate the prognostic and predictive significance of this serum biomarker in MBC. Methods: Serial CTC levels are obtained in patients starting a new systemic treatment regimen for progressive, radiographically measurable MBC. 10 mL samples of peripheral blood are collected before the start of treatment and then at 3–4 week intervals. All subjects are followed prospectively for PFS and OS, and they are offered the opportunity to continue CTC testing upon disease progression. CTC enumeration is performed on a 7.5 mL blood volume using the CellSearch technology (Veridex, LLC; Warren, NJ). Epithelial cells are immunomagnetically separated and fluorescently labeled, and nucleated (DAPI+) cells with the EpCAM+, cytokeratin 8/18/19+, and CD45- phenotype are counted as CTC. Clinical outcomes are based on radiographic studies and physical examination in accordance with RECIST criteria. Results: 46 of 100 subjects have been accrued, and 33 have completed at least one radiographic staging evaluation with a median follow up of 7 mos (range 2–18 mos). Treatment for the 33 evaluable patients includes chemotherapy (27%), endocrine therapy (46%), and combination therapy with a biologic agent (27%). At baseline, 85% (28/33) had at least 1 CTC/7.5 mL (range 1–78), and 27% (9/33) had ≥5 CTC/7.5 mL. Median PFS was 2.57 months and 6.77 months for subjects with ≥5 vs <5 CTC/7.5 mL at baseline, respectively (p=0.02). Conclusions: The current data validate the observation that baseline CTC levels correlate with PFS in patients with MBC and measurable disease. Patient accrual and data analysis are ongoing to confirm that persistent CTC levels ≥5/7.5 mL correlate with a lack of treatment efficacy and therefore are a reliable surrogate marker of disease responsiveness and PFS. [Table: see text]
41
Falco, J. R. P., and M. L. S. Mello. "Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants." Genetics and Molecular Biology 22, no. 2 (June 1999): 197–200. http://dx.doi.org/10.1590/s1415-47571999000200010.
Full textAbstract:
The critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in DNA-protein complexes but is of little phylogenetic value.
42
Cristiano, Antonio, Valentina Fortunati, Fabio Cherubini, Sergio Bernardini, and Marzia Nuccetelli. "Anti-phospholipids antibodies and immune complexes in COVID-19 patients: a putative role in disease course for anti-annexin-V antibodies." Clinical Rheumatology 40, no. 7 (January 19, 2021): 2939–45. http://dx.doi.org/10.1007/s10067-021-05580-3.
Full textAbstract:
Abstract Introduction Besides distinctive respiratory and digestive hallmarks, COVID-19 has been recently associated with a high prevalence of pro-inflammatory and hypercoagulable states known as “COVID-19 Associated Coagulopathy” (CAC), corresponding to a worsening in patients’ conditions, whose causes are still to be elucidated. A link between anti-phospholipid antibodies (aPLs) and viral infections has long been suggested. APLs are assessed for anti-phospholipid syndrome (APS) diagnosis, characterized by thrombocytopenia, thrombosis, and coagulopathy. Furthermore, circulating immune complexes (CICs), arisen upon inflammatory responses and related immune dysregulation, can lead to endothelial cell damage and thrombotic complications. Method We performed an extended panel including IgG/IgM anti-cardiolipin, IgG/IgM anti-β2-glycoprotein-1, coupled with IgG/IgM anti-prothrombin, IgG/IgM anti-annexin-V on two COVID-19 patient groups (early and late infection time), and a negative control group. IgG CIC analysis followed to evaluate inflammatory status, through a possible complement system activation. Results Our results showed low positive case percentage in IgG/IgM anti-cardiolipin and IgG/IgM anti-β2-glycoprotein-1 assays (4.54%, 6.25%, and 4.55%; in early infection group, late infection group, and control group, respectively); few positive cases in IgG/IgM anti-prothrombin and IgG/IgM anti-annexin-V immunoassays; and no IgG CIC positivity in any patient. Conclusions In conclusion, our data show a low aPL prevalence, likely excluding an involvement in the pathogenesis of CAC. Interestingly, IgG/IgM anti-prothrombin and anti-annexin-V positive cases, detected in late infection group, suggest that aPLs could temporarily increase or could trigger a “COVID-19-induced-APS-like-syndrome” in predisposed patients. Key Points•To our knowledge, anti-prothrombin (aPT) antibodies, anti-annexin-V antibodies and CICs in COVID-19 patients have not been reported in the scientific literature.•Lack of uniformity and the low percentage of aCL/aβ2GP1 positivity preclude a putative role in CAC pathogenesis.•IgG/IgM anti-prothrombin and IgG/IgM anti-annexin-V data show that distribution of positive case number increases in late infection patients, significantly in anti-annexin-V results, suggesting a possible role for these anti-phospholipid antibodies in disease course.•aPLs can arise transiently in some patients with critical illness and SARS-CoV-2 infection (disappearing in a few weeks), as well as in other genetically predisposed patients; they could trigger a “COVID-19-induced-APS-like-syndrome”.
43
Ferreira, Francisco J. F., Luiz Fornazzari Neto, Luizemara S. A. Szameitat, Gilson B. Guimarães, Victor M. Oliveira Martin, Hélcio Prazeres Filho, and Horstpeter H. Ulbrich. "GAMMA-RAY SPECTROMETRY AS A TOOL FOR MAPPING PETROGRAPHIC DOMAINS IN GRANITOIDS: THE EXAMPLES OF THE CUNHAPORANGA AND TRÊS CÓRREGOS GRANITIC COMPLEXES, PARANÁ STATE, SOUTHERN BRAZIL." Revista Brasileira de Geofísica 32, no. 3 (September 1, 2014): 465. http://dx.doi.org/10.22564/rbgf.v32i3.521.
Full textAbstract:
ABSTRACT. The Aerogeophysical Project Serra do Mar Sul compiled gamma-ray data in the 70’s, for a large basement area (50,000 km2) in Paraná state, southernBrazil. The cps data were corrected in 1997 by BARMP, Brazil Airborne Radiometric Mapping Project, and are available now as TC (eU), K (%), eTh and eU (in ppm). Thedata for the two large intrusions in the region, the Cunhaporanga Granitic Complex (CGC) and Trˆes C´orregos Granitic Complex (TCGC) were used to generate maps forK, eTh, eU, total counts, and several derived parameters: eTh/K, eU/K, eU/eTh, F. Contrasts between the two complexes are explained as a result of outcrop pattern andweathering (CGC more weathered than TCGC) and differences in overall Th content (eastern TCGC domains richer in the element), but a more uniform U distribution.The differences are enhanced in the eTh/K map (TCGC with lower ratios). The late alaskitic Serra do Carambe´ı intrusion in the CGC shows the highest Th and U values.Gamma-ray patterns in granites subjected to strong subtropical alterations, with heavy seasonal rains, are more difficult to interpret, considering the possibility of strongleaching of K by weathering, and also supergene mobility of U, factors that are absent, or less important, in areas with drier climates. The gamma-ray patterns of thethree elements are influenced by their original distribution in the granites, but can be drastically changed by late hydrothermal alteration and weathering.Keywords: granitic complexes, gamma-ray spectrometric maps, BARMP. RESUMO. O Projeto Aerogeofísico Serra do Mar Sul, da década de 70, gerou mapas gamaespectrométricos para uma região de 50.000 km2 de embasamentocristalino no Estado do Paraná, Brasil meridional. Valores em cps foram corrigidos para TC (eU), K (%), eTh e eU (em ppm) no Brazil Airborne Radiometric MappingProject, BARMP, em 1997. Dados para as grandes intrusões da região, os complexos Graníticos Cunhaporanga (CGC) e Três Córregos (TCGC), foram utilizadospara gerar mapas de K, eTh, eU, contagem total e dos parâmetros eTh/K, eU/K, eU/eTh, F. Os contrastes entre os dois complexos são explicados pelo padrão de afloramentos e intemperismo (CGC mais intemperizado que TCGC) e diferenças no teor geral de Th (domínios leste do TCGC enriquecidos no elemento) mais que do U. Estas diferenças são salientadas no mapa eTh/K (TCGC com razões menores). O Granito Serra do Carambeí, uma intrusão alasquítica tardia no CGC, mostra os teoresmais elevados de Th e U. Padrões de raios gama em granitos submetidos a um acentuado intemperismo subtropical (com fortes chuvas sazonais) são de interpretação mais dif´ícil que os de regiões com climas mais secos, em razão da possibilidade de lixiviamento total de K por intemperismo, e mobilidade supêrgena do U. Essesmapas indicam o padrão geral de distribuição de K, Th e U, eventualmente com mudanças drásticas impostas por alteração hidrotermal e especialmente intemperismo.Palavras-chave: complexos graníticos, mapas gamaespectrométricos, BARMP.
44
Hoetger, Cosima, Rose S. Bono, Nicole E. Nicksic, Andrew J. Barnes, and Caroline O. Cobb. "Influence of Electronic Cigarette Characteristics on Susceptibility, Perceptions, and Abuse Liability Indices among Combustible Tobacco Cigarette Smokers and Non-Smokers." International Journal of Environmental Research and Public Health 16, no. 10 (May 23, 2019): 1825. http://dx.doi.org/10.3390/ijerph16101825.
Full textAbstract:
This study assessed how electronic cigarette (ECIG) characteristics amenable to regulation—namely nicotine content, flavor, and modified risk messages—impact ECIG use susceptibility, harm/addiction perceptions, and abuse liability indices among combustible tobacco cigarette (CTC) smokers and non-smokers. CTC smokers and non-smokers varying in ECIG use recruited via Amazon Mechanical Turk (MTurk) completed an online survey in 2016 (analytic n = 706). Participants were randomly assigned to one of eight conditions differing in ECIG characteristics: nicotine content (no, low, high), flavor (menthol, tobacco, fruit), or modified risk message (reduced harm, reduced carcinogen exposure). Regressions assessed ECIG susceptibility, harm/addiction perceptions, and abuse liability indices (purchase task measures of breakpoint/intensity) within each regulatory domain (nicotine content, flavor, message) and their interactions with CTC/ECIG status. Differential effects on ECIG susceptibility, harm/addiction perceptions, and abuse liability indices were observed by regulatory domain with many effects moderated by CTC/ECIG status. ECIG nicotine content and flavor conditions were the most influential across outcomes. Greater nicotine content, tobacco-flavored and reduced carcinogen exposure ECIGs were more highly preferred by CTC smokers with some differing preferences for non-users. Findings reinforce consideration of discrete ECIG preferences across tobacco use status to improve regulatory efficacy.
45
Silva, Virgílio Souza e., Emne Ali Abdallah, Angelo Borsarelli Carvalho de Brito, Alexcia Camila Braun, Milena Shizue Tariki, Celso Abdon Lopes de Mello, Vinicius Fernando Calsavara, Rachel Riechelmann, and Ludmilla Thomé Domingos Chinen. "Baseline and Kinetic Circulating Tumor Cell Counts Are Prognostic Factors in a Prospective Study of Metastatic Colorectal Cancer." Diagnostics 11, no. 3 (March 12, 2021): 502. http://dx.doi.org/10.3390/diagnostics11030502.
Full textAbstract:
The discovery of predictive biomarkers in metastatic colorectal cancer (mCRC) is essential to improve clinical outcomes. Recent data suggest a potential role of circulating tumor cells (CTCs) as prognostic indicators. We conducted a follow-on analysis from a prospective study of consecutive patients with mCRC. CTC analysis was conducted at two timepoints: baseline (CTC1; before starting chemotherapy), and two months after starting treatment (CTC2). CTC isolation/quantification were completed by ISET® (Rarecells, France). CTC expressions of drug resistance-associated proteins were evaluated. Progression-free survival (PFS) and overall survival (OS) were estimated by the Kaplan–Meier method. Seventy-five patients were enrolled from May 2012 to May 2014. A CTC1 cut-off of >1.5 CTCs/mL was associated with an inferior median OS compared to lower values. A difference of CTC2−CTC1 > 5.5 CTCs/mL was associated with a reduced median PFS. By multivariate analysis, CTC1 > 1.5 CTCs/mL was an independent prognostic factor for worse OS. Multi-drug resistance protein-1 (MRP-1) expression was associated with poor median OS. CTC baseline counts, kinetics, and MRP-1 expression were predictive of clinical outcomes. Larger studies are warranted to explore the potential clinical benefit of treating mCRC patients with targeted therapeutic regimens guided by CTC findings.
46
Lucena, D. V., L. V. Amorim, and H. L. Lira. "Análise da reatividade de folhelhos do Recôncavo Baiano." Cerâmica 62, no. 362 (June 2016): 163–69. http://dx.doi.org/10.1590/0366-69132016623621976.
Full textAbstract:
Resumo O problema da estabilidade do poço em formações expansivas frustrou os engenheiros de campos de petróleo desde o início da exploração de petróleo e gás natural. A estabilidade de folhelhos ricos em argila é profundamente afetada por suas interações físicas e químicas complexas com fluidos de perfuração. Assim, estas rochas com alto teor de argila mostraram alterações significativas, tais como inchaço ou expansão, quando colocadas em contato com fluidos aquosos para a adsorção de moléculas polares de água ou íons hidratados dissolvidos no meio. Essas mudanças podem causar colapso da rocha durante a perfuração com fluidos de perfuração à base de água. Para uma melhor compreensão dos fenômenos de interação entre a rocha e o fluido de perfuração, este trabalho objetiva a análise da reatividade de folhelhos provenientes do Recôncavo Baiano por meio da sua caracterização. Para isto, os folhelhos foram submetidos a ensaios de CTC, FRX, DRX e inchamento de Foster. Foi possível detectar que os folhelhos apresentaram significativos valores de CTC, bem como FRX com composição próxima da esmectita e padrão de DRX com picos característicos da esmectita. Em relação ao inchamento de Foster, o folhelho com maior CTC também apresentou o maior valor de inchamento indicando que a CTC pode ser aplicada como parâmetro indicativo da reatividade.
47
Babintseva, Yanina D., A. M. Sergeeva, V. P. Karagodin, and A. N. Orekhov. "Atherogenesis in human - clinical aspects of circulating immune complexes." Clinical Medicine (Russian Journal) 94, no. 5 (June 20, 2016): 325–32. http://dx.doi.org/10.18821/0023-2149-2016-94-5-325-332.
Full textAbstract:
It has been suggested that circulating immune complexes containing low density lipoproteins (LDL-CIC) play a role in atherogenesis and are involved in the formation of early atherosclerotic lesions. The complexes, as well as anti-LDL antibody were found in the blood of patients with atherosclerotic process in various cardiovascular diseases, well as in the blood of animals with experimentally modulated atherosclerosis. One can assume that the presence anti-LDL antibodies in blood is a result of an immune response that is induced by modification of lipoproteins. LDL-CIC differ from native LDL in many aspects. They have much lower levels of sialic acid, a smaller diameter and a higher density electronegativity than native LDL. The fraction of the LDL-CIC in serum is an important manifestation of the atherosclerotic process. LDL-CIC, unlike the native LDL is able to induce intracellular accumulation of neutral lipids, especially esterified cholesterol in cell cultures obtained from healthy human aortic intima and macrophages in culture. After removal of the LDL-CIC, the serum of CHD-patients loses its atherogenic properties. The titer of the LDL-CIC in the blood serum significantly correlate with the progression of atherosclerosis and in vivo has the highest diagnostic yield of measured among other lipid parameters. Increasing CIC- cholesterol could also increase the risk of coronary artery atherosclerosis.
48
Furuhata, Masahiko, Radostin Danev, Kuniaki Nagayama, Yoshifumi Yamada, Hiroko Kawakami, Kazunori Toma, Yoshiyuki Hattori, and Yoshie Maitani. "Decaarginine-PEG-Artificial Lipid/DNA Complex for Gene Delivery: Nanostructure and Transfection Efficiency." Journal of Nanoscience and Nanotechnology 8, no. 5 (May 1, 2008): 2308–15. http://dx.doi.org/10.1166/jnn.2008.170.
Full textAbstract:
Oligoarginine conjugates are highly efficient vectors for the delivery of plasmid DNA into cells. Decaarginine-conjugated lipid (Arg10-PEG-lipid) was synthesized and the effects of Arg10-PEG-lipid concentration at a fixed DNA concentration on transfection efficiency and the structure of the complexes were studied below and above critical micelle concentration (CMC), and at the lipid nitrogen/DNA phosphate (N/P) ratio corresponding to transfection, respectively. Arg10-PEG-lipid at the concentration below CMC showed stronger interaction with DNA by fluorescence intensity distribution analysis, and significantly higher luciferase and green fluorescent protein expression than that above CMC. A phase-contrast cryo-transmission electron microscope (cryo-TEM) experiment showed that the morphology of the complexes depended on the N/P ratio. At a low N/P ratio corresponding to that in transfection at a lipid concentration below CMC, a net-like structure developed in which plasmid DNA was involved. A further increase in the N/P ratio, a large fibrous nanostructure of complexes, was also observed. Without DNA, these structures were not obtained. The cellular uptake mechanism of complexes using flow cytometry with inhibitors suggested that complexes with two different morphologies showed similar cellular uptake and uptake mechanism, macropinocytosis. Differences in transfection efficiency of the complexes may be explained by a large fibrous nanostructure inhibiting the cellular internalization of complexes or the release of DNA from macropinosomes into cytoplasm. Arg10-PEG-lipid/DNA complexes formed a favorable nanostructure for gene delivery, depending on the N/P ratio in water.
49
Nakakoji, M., and H. Funahashi. "265 HYALURONAN SYNTHESIS ABILITY OF PORCINE CUMULUS - OOCYTE COMPLEXES DERIVED FROM SMALL FOLLICLES." Reproduction, Fertility and Development 25, no. 1 (2013): 280. http://dx.doi.org/10.1071/rdv25n1ab265.
Full textAbstract:
The degree of cumulus expansion, an important step in oocyte maturation, of porcine cumulus–oocyte complexes (COC) derived from small follicles (SF: 1 to 2 mm in diameter) is known to be lower than those derived from middle follicles (MF: 3 to 6 mm in diameter). The objective of this study was to compare the abilities of hyaluronan (HA) synthesis of COC from SF and MF. Furthermore, the effect of oestradiol during pre-incubation of COC on proliferation of the cumulus cells was examined. Cumulus–oocyte complexes from SF and MF of porcine ovaries were cultured for in vitro maturation [IVM, in modified porcine oocyte medium (Yoshioka et al. 2008 J. Reprod. Dev. 54, 208–213) supplemented with 50 µM β-mercaptoethanol, 10 IU mL–1 of eCG, 10 IU mL–1 of hCG, and 1 mM dbcAMP for 20 h and then in the fresh medium without those supplements for another 24 h]. Hyaluronan production was quantified at 20 h after the start of IVM with a commercial HA-ELISA kit (20 COC/tube × 4 times). The number of cumulus cells was assessed 0 and 20 h after the start of IVM (50 COC × 4 times). Furthermore, proliferation of cumulus cells was examined after pre-culture of COC (n = 40 COC × 5 times) in modified porcine oocyte medium with various concentrations of oestradiol (0, 0.1, 1, and 10 ng mL–1) for 6 h. Statistical analyses of results from 4 to 5 replicated trials were performed by ANOVA with a Bonferroni-Dunn post-hoc test (significance, P < 0.05). The degree of cumulus expansion of COC from MF (n = 152) was higher than that of COC from SF (n = 156). The incidence of metaphase-II oocytes was significantly lower in COC from SF (n = 133; 48.9%) than in COC from MF (n = 148; 74.7%). The HA content of COC was higher in those from MF (20.8 µg/COC) than in those from SF (10.8 µg/COC), whereas the content per cumulus cell was not different because the numbers of cumulus cells at 0 and 20 h were also higher in COC (n = 200 in each group) from MF (3.0 × 103 and 3.3 × 103 cells, respectively) than from SF (2.0 × 103 and 2.5 × 103 cells, respectively). Cumulus cells proliferated significantly in the presence of oestradiol, regardless of the concentration, during pre-incubation for 6 h (2.5 to 2.8 × 103 cells), as compared with the oestradiol-free controls (2.2 × 103 cells). These results demonstrate that the different abilities of cumulus expansion between COC (n = 200 in each group) from SF and MF may be due to the number of cumulus cells per COC. Pre-incubation in the presence of oestradiol stimulates the proliferation of cumulus cells and may improve the oocyte maturation of COC derived from SF.
50
González-Fernández, L., M. J. Sánchez-Calabuig, M. G. Alves, P. F. Oliveira, S. Macedo, A. Gutiérrez-Adán, A. Rocha, and B. Macías-García. "Expanded equine cumulus–oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus–oocyte complexes." Reproduction, Fertility and Development 30, no. 2 (2018): 297. http://dx.doi.org/10.1071/rd16441.
Full textAbstract:
Equine cumulus–oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30 h, at which time maturation rate, glucose metabolism and the expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. There were significant differences between eCOCs and cCOCs in maturation rate (50% vs 21.7% (n = 192 and 46) respectively; P < 0.001), as well as mean (± s.e.m.) glucose consumption (1.8 ± 0.5 vs 27.9 ± 5.9 nmol per COC respectively) and pyruvate (0.09 ± 0.01 vs 2.4 ± 0.8 nmol per COC respectively) and lactate (4.7 ± 1.3 vs 64.1 ± 20.6 nmol per COC respectively; P < 0.05 for all) production. Glucose consumption in EC and CC did not differ significantly. Expression of hyaluronan-binding protein (tumour necrosis factor alpha induced protein 6; TNFAIP6) was increased in eCOCs and EC, and solute carrier family 2 member 1 (SLC2A1) expression was increased in eCOCs, but there were no differences in the expression of glycolysis-related enzymes and solute carrier family 2 member 3 (SLC2A3) between the COC or mural granulosa cell types. The findings of the present study demonstrate that metabolic and genomic differences exist between eCOCs and cCOCs and mural granulosa cells in the horse.