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Journal articles on the topic 'Concentration cell'

Author: Grafiati

Published: 9 March 2023

Last updated: 31 July 2025

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1

Kusuma, Riska Anggri, Linda Suyati, and Wasino Hadi Rahmanto. "Effect of Lactose Concentration as Lactobacillus bulgaricus Substrate on Potential Cells Produced in Microbial Fuel Cell Systems." Jurnal Kimia Sains dan Aplikasi 21, no. 3 (2018): 144–48. http://dx.doi.org/10.14710/jksa.21.3.144-148.

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The effect of laxose concentration as Lactobacillus bulgaricus bacterial substrate on the cell potential produced in Microbial Fuel Cell System has been done. This study aims to determine the effect of lactose concentration as bacterial substrate, to generate electricity, maximum electric potential and determine the potential value of standard lactose (E ° Lactose.) Based on Nernst equation. The MFC system of two compartments and bridges of salt as a linkage is used in this study. Anode contains lactose with variation of concentration 3 - 7% and bacteria. The cathode contains a 1M KMO4. The el

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Kisitu, Jaffar. "Chemical concentrations in cell culture compartments (C5) – concentration definitions." ALTEX 36, no. 1 (2019): 154–60. http://dx.doi.org/10.14573/altex.1901031.

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3

Z, Ding. "Concentration Polarization of Ox-LDL and Its Effect on Cell Proliferation and Apoptosis in Human Endothelial Cells." Journal of Cardiology and Cardiovascular Medicine 1, no. 1 (2016): 011–18. http://dx.doi.org/10.29328/journal.jccm.1001003.

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4

Tong, Xiwen, Xiaojian Zhu, Su Shuai, et al. "Impact of Selinexor on AML Cell Lines and Healthy Donor T Cell Function: Enhanced Treg Cell Proportion." Blood 144, Supplement 1 (2024): 5817. https://doi.org/10.1182/blood-2024-205174.

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Introduction: Selinexor is an oral, first-in-class selective inhibitor of nuclear export (SINE), specifically blocking XPO1. Selinexor has shown promising results in maintenance therapy for post-transplant AML and MDS . However, its specific impact on T cell function in post-transplant patients remains unclear. Therefore, this study conducted a series of investigations into the effects of selinexor on T cell function. Methods: Effects of selinexor on proliferation and apoptosis of four types of AML cells and FLT3-mutant AML cells detected by CCK-8 assay and flow cytometry. The cytotoxicity, ex

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Kiuchi, Tai, Tomoaki Nagai, Kazumasa Ohashi, and Kensaku Mizuno. "Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension." Journal of Cell Biology 193, no. 2 (2011): 365–80. http://dx.doi.org/10.1083/jcb.201101035.

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To understand the intracellular role of G-actin concentration in stimulus-induced actin assembly and lamellipodium extension during cell migration, we developed a novel technique for quantifying spatiotemporal changes in G-actin concentration in live cells, consisting of sequential measurements of fluorescent decay after photoactivation (FDAP) of Dronpa-labeled actin. Cytoplasmic G-actin concentrations decreased by ∼40% immediately after cell stimulation and thereafter the cell area extended. The extent of stimulus-induced G-actin loss and cell extension correlated linearly with G-actin concen

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Jia, Chen, Abhyudai Singh, and Ramon Grima. "Concentration fluctuations in growing and dividing cells: Insights into the emergence of concentration homeostasis." PLOS Computational Biology 18, no. 10 (2022): e1010574. http://dx.doi.org/10.1371/journal.pcbi.1010574.

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Intracellular reaction rates depend on concentrations and hence their levels are often regulated. However classical models of stochastic gene expression lack a cell size description and cannot be used to predict noise in concentrations. Here, we construct a model of gene product dynamics that includes a description of cell growth, cell division, size-dependent gene expression, gene dosage compensation, and size control mechanisms that can vary with the cell cycle phase. We obtain expressions for the approximate distributions and power spectra of concentration fluctuations which lead to insight

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Trifilio, Steven M., Paul R. Yarnold, Marc H. Scheetz, Judy Pi, Gennethel Pennick, and Jayesh Mehta. "Serial Plasma Voriconazole Concentrations after Allogeneic Hematopoietic Stem Cell Transplantation." Antimicrobial Agents and Chemotherapy 53, no. 5 (2009): 1793–96. http://dx.doi.org/10.1128/aac.01316-08.

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ABSTRACT Plasma voriconazole concentrations vary considerably between patients receiving standard dosing, and trough voriconazole concentrations are known to affect efficacy and toxicity. Temporal variations in serial plasma voriconazole concentrations through the course of therapy in hematopoietic stem cell transplantation patients has not been carefully described. Paired voriconazole concentrations in 64 patients were studied to determine the predictability of the second concentration based on the first. The difference between the two values was ≤5% in six patients. In 25 patients, the secon

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Rowley, SD, WI Bensinger, TA Gooley, and CD Buckner. "Effect of cell concentration on bone marrow and peripheral blood stem cell cryopreservation." Blood 83, no. 9 (1994): 2731–36. http://dx.doi.org/10.1182/blood.v83.9.2731.2731.

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Abstract The effects of cell concentration during cryopreservation on bone marrow (BM) or peripheral blood (PB)-derived hematopoietic progenitor cells have not been described. The much greater numbers of cells harvested for autologous PB stem cell (PBSC) transplantation requires that the cells be frozen at higher cell concentrations, or in much greater volumes, compared with BM. We cryopreserved 108 PBSC collections from 30 patients at an average (+/- SD) cell concentration of 3.7 +/- 1.9 x 10(8) nucleated cells per mL in 127 +/- 45 mL. The proportion of mononuclear cells was 52.9% +/- 27.2%.

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Rowley, SD, WI Bensinger, TA Gooley, and CD Buckner. "Effect of cell concentration on bone marrow and peripheral blood stem cell cryopreservation." Blood 83, no. 9 (1994): 2731–36. http://dx.doi.org/10.1182/blood.v83.9.2731.bloodjournal8392731.

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The effects of cell concentration during cryopreservation on bone marrow (BM) or peripheral blood (PB)-derived hematopoietic progenitor cells have not been described. The much greater numbers of cells harvested for autologous PB stem cell (PBSC) transplantation requires that the cells be frozen at higher cell concentrations, or in much greater volumes, compared with BM. We cryopreserved 108 PBSC collections from 30 patients at an average (+/- SD) cell concentration of 3.7 +/- 1.9 x 10(8) nucleated cells per mL in 127 +/- 45 mL. The proportion of mononuclear cells was 52.9% +/- 27.2%. The produ

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Kočí, Vladimír, Darek Dragoun, and Jaromír Lukavský. "Determination of algal cell culture (Desmodesmus subspicatus) concentration using a microplate reader." Algological Studies/Archiv für Hydrobiologie, Supplement Volumes 122 (December 1, 2006): 123–35. http://dx.doi.org/10.1127/1864-1318/2006/0122-0123.

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11

Salmman, Israa Sekar. "Effect crude aquatic extract of leaves Ocimum basilicum on caner and normal cell lines in vitro." Journal of Biotechnology Research Center 7, no. 1 (2013): 5–13. http://dx.doi.org/10.24126/jobrc.2013.7.1.233.

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Leaves of Ocimum basilicum were extracted with distilled water to prepare aquatic crude extract, five concentration from this extract (1000, 500, 250, 125, 62.5)µg/ml were used to study the effect of extract on cancer cell lines(Larynex carcinoma hep-2, cervix carcinoma Hela and mammary gland adenocarcinoma AMN-3) and the time exposure 24 and 48 hours as well as the effect of aquous extract on normal cell line for embryonic mice fibroblast(MEF) was studied in vitro at 48hrs. exposure. The result showed that aqueous crude extract of Ocimum basilicum leaves has different effects on cancer cell l

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12

Gülden, M., S. Mörchel, and H. Seibert. "Factors influencing nominal effective concentrations of chemical compounds in vitro: cell concentration." Toxicology in Vitro 15, no. 3 (2001): 233–43. http://dx.doi.org/10.1016/s0887-2333(01)00008-x.

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13

Feng, Zhenyu, Chao Chen, Zhenyu Ye, et al. "The Effect of Icariin on Human Hepatoellular Carcinomas Cell Apoptosis and Cell Cycle Arrest." Journal of Biomaterials and Tissue Engineering 11, no. 12 (2021): 2389–94. http://dx.doi.org/10.1166/jbt.2021.2814.

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Background: Natural products often have novel frameworks and unique mechanisms, It is an important way to develop new anti-tumor drugs.The paper explored the effect of Icariin on HepG2 cell apoptosis and cell cycle arrest. Material and methods: Human liver cancer HepG2 cells were studied. The biological activity of Icariin on HepG2 cells was comprehensively investigated. The mechanism was preliminarily explored from the aspects of proliferation, cell cycle, and apoptosis. Results: Icariin showed significant inhibitory effect on cell proliferation after administration, and the effect was time-a

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Kempe, Hermannus, Anne Schwabe, Frédéric Crémazy, Pernette J. Verschure, and Frank J. Bruggeman. "The volumes and transcript counts of single cells reveal concentration homeostasis and capture biological noise." Molecular Biology of the Cell 26, no. 4 (2015): 797–804. http://dx.doi.org/10.1091/mbc.e14-08-1296.

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Transcriptional stochasticity can be measured by counting the number of mRNA molecules per cell. Cell-to-cell variability is best captured in terms of concentration rather than molecule counts, because reaction rates depend on concentrations. We combined single-molecule mRNA counting with single-cell volume measurements to quantify the statistics of both transcript numbers and concentrations in human cells. We compared three cell clones that differ only in the genomic integration site of an identical constitutively expressed reporter gene. The transcript number per cell varied proportionally w

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Katsogiannou, EG, PD Katsoulos, C. Ziogas, et al. "Blood cell count and morphology, and vitamin B12 concentration in pre- and post-weaned calves." Veterinární Medicína 66, No. 12 (2021): 513–19. http://dx.doi.org/10.17221/12/2021-vetmed.

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Haematological indicators may resent physiological variation by age. Vitamin B12 promotes haematopoiesis. The aims of this study were: 1) to compare the values of the haematological variables and the concentration of vitamin B12 in pre- or post-weaned veal calves and 2) to identify the possible association between the values of the haematological variables and the concentration of B12 in the blood of veal calves. Blood was collected on the same day from 31 pre-weaned and 31 weaned calves of the Limousine breed from the same farm. The complete blood count, including the blood cell morphology ev

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Nagarad Dasavandi Krishnamurthy, Vinay. "Hydrogen Concentration Sensors in Fuel Cell Electric Vehicles: Design Considerations, Advancements and Challenges." International Journal of Science and Research (IJSR) 11, no. 2 (2022): 1335–38. http://dx.doi.org/10.21275/sr24517162834.

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17

Liu, Sheng-Long, Lu Yang, Cheng-Jun Zhu, Kai Liu, Wei Han, and Jia-Feng Yao. "A method of identifying cell suspension concentration based on bioimpedance spectroscopy." Acta Physica Sinica 71, no. 7 (2022): 078701. http://dx.doi.org/10.7498/aps.71.20211837.

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Based on bioimpedance spectroscopy technology, a method of automatically identifying the cell suspension concentration is proposed. This method combines multiple linear regression algorithm and bioimpedance spectroscopy technology, which can identify the concentration of cell suspension quickly and accurately. Firstly, a strategy of random distribution of cell locations is proposed to simulate the true existence of cells. Secondly, 2400 groups of normal, cancerous and mixed cell models with different concentrations are generated by numerical simulation and their bioimpedance spectroscopy data

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18

Kamath, Meghana, Isaac Houston, Alexander Janovski, et al. "Myeloid Gene Activation and T Cell/Natural Killer Cell Gene Repression in Cells Expressing Two Distinct PU.1 Concentrations." Blood 110, no. 11 (2007): 1242. http://dx.doi.org/10.1182/blood.v110.11.1242.1242.

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Abstract The Ets transcription factor PU.1 (encoded by the gene Sfpi1) functions in a concentration-dependent manner as a hematopoietic cell fate determinant. PU.1 levels are uniform in early hematopoiesis, increase during myeloid differentiation, and decrease after erythrocyte and T cell/natural killer cell commitment. It is unknown how downstream target genes respond to changes in PU.1 concentration. To address this, we generated mice with two distinct hypomorphic alleles of Sfpi1 and analyzed interleukin-3 dependent cell lines from fetal liver cells homozygous for either allele. PU.1 was pr

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Kamal, Achmad Fauzi, Akbar Rizki Beni Asdi, Ahmad Jabir Rahyussalim, et al. "Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study." Stem Cells International 2020 (September 1, 2020): 1–10. http://dx.doi.org/10.1155/2020/8827192.

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Background. Various chemical agents have been used as an adjuvant treatment for giant cell tumor (GCT). However, the comparative effect of these chemicals remains unclear. Methods. Multinucleated and spindle cells from cultured GCT patients, characterized by Nanog and Oct4 expression with RT-PCR, were directly administered, in vitro, with concentrations of 1%, 3%, and 5% of H2O2 and 75%, 85%, and 95% of ethanol for 10 minutes and concentrations of 0.003%, 0.005%, 0.01%, 0.03%, 0.1%, and 0.3% of H2O2 for 5 minutes and were incubated for 24 hours. Cell morphology, cell viability, and flow cytome

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20

Han, Song-I., Hyun Soo Kim, and Arum Han. "In-droplet cell concentration using dielectrophoresis." Biosensors and Bioelectronics 97 (November 2017): 41–45. http://dx.doi.org/10.1016/j.bios.2017.05.036.

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21

Kilbride, Peter, Julie Meneghel, and John Morris. "Cell Concentration And Sedimentation In Cryopreservation." Cryobiology 91 (December 2019): 193. http://dx.doi.org/10.1016/j.cryobiol.2019.10.180.

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22

Hassan, Adnan F., Ibtihaj R. Alshirmani, and Ali H. khwayyir. "Pyranine Dye as Solar Cell Concentration." International Journal of Applied Physics 6, no. 2 (2019): 73–78. http://dx.doi.org/10.14445/23500301/ijap-v6i2p111.

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23

Loges, K. M., P. Wiedemann, B. Hitzmann, and K. Preuß. "Automated cell concentration control in bioreactors." Chemie Ingenieur Technik 92, no. 9 (2020): 1337–38. http://dx.doi.org/10.1002/cite.202055091.

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24

Sheehan, D. P. "A Self-Charging Concentration Cell: Theory." Batteries 9, no. 7 (2023): 372. http://dx.doi.org/10.3390/batteries9070372.

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Batteries are a key resource in the quest for sustainable energy. Here, the theoretical basis is presented for a new type of electrochemical concentration cell that might contribute to this enterprise. The cell, which has been successfully demonstrated in the laboratory, incorporates a chemically asymmetric membrane to drive anisotropic diffusion between two solution chambers; the resulting concentration difference powers the cell. In this study, the membrane’s operation is validated via three theoretical approaches: (i) traditional equilibrium thermodynamics; (ii) balancing drift and diffusio

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Ismiyati, Titik, Widowati Siswomihardjo, Marsetyawan H. N. E. Soesatyo, and Rochmadi Rochmadi. "UJI SITOKSISITAS CAMPURAN RESIN AKRILIK DENGAN KITOSAN SEBAGAI BAHAN GIGI TIRUAN ANTI JAMUR." Jurnal Teknosains 5, no. 2 (2017): 97. http://dx.doi.org/10.22146/teknosains.27580.

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Denture acrylic resin contain monomer residue that can cause allergic reactions and inflammation in the mouth. Chitosan has advantage biocompatible and antifungal. The purpose of this study was to examine toxicity acrylic resin blends with chitosan as denture antifungal in fibroblast cell culture. This study uses chitosan concentration 0.5%, 1%, 2% and 4% of 2.5 ml were blends with acrylic resin. Fibroblast cell cellular responses were assessed using MTT assay. Data at the cell viability analyzes used Anova one path (p <0.05). The results showed the greatest average adsorbansi fibroblast ce

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Eberhardt, S. H., F. Marone, M. Stampanoni, F. N. Büchi, and T. J. Schmidt. "Quantifying phosphoric acid in high-temperature polymer electrolyte fuel cell components by X-ray tomographic microscopy." Journal of Synchrotron Radiation 21, no. 6 (2014): 1319–26. http://dx.doi.org/10.1107/s1600577514016348.

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Synchrotron-based X-ray tomographic microscopy is investigated for imaging the local distribution and concentration of phosphoric acid in high-temperature polymer electrolyte fuel cells. Phosphoric acid fills the pores of the macro- and microporous fuel cell components. Its concentration in the fuel cell varies over a wide range (40–100 wt% H3PO4). This renders the quantification and concentration determination challenging. The problem is solved by using propagation-based phase contrast imaging and a referencing method. Fuel cell components with known acid concentrations were used to correlate

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Naundorf, Gerardo, and Nicholas G. Aumen. "Ammonia-induced cell envelope injury in Escherichia coli and Enterobacter aerogenes." Canadian Journal of Microbiology 36, no. 8 (1990): 525–29. http://dx.doi.org/10.1139/m90-092.

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Ammonia-induced cell envelope injury was examined in pure cultures of Escherichia coli and Enterobacter aerogenes. Cell injury, as determined by the ratio of colony-forming units on m-T7 agar to colony-forming units on m-Endo agar, increased with exposure to increasing concentrations of ammonia. Cell envelopes appeared to be the site of injury as indicated by increasing susceptibility to lysozyme with increasing ammonia concentration. Cells exposed to ammonia also exhibited more cellular leakage than control cells. Leakage from cells exposed to ammonia included proteins, and all leaked substan

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Al-Zeiny, Saadia Saleh Mehdy, Kifah Jabbar Alyaqubi, and Duraid Abdul Hadi Abbas. "In vitro: anticancer effect of oily and methanolic extracts of Al-Zahdi (Phoenix dactylifera L.) from dry dates and leaves on AMN3, Hela and Ref cancer cell cultures." Kufa Journal For Veterinary Medical Sciences 13, no. 2 (2022): 1–12. http://dx.doi.org/10.36326/kjvs/2022/v13i23303.

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The antioxidant and anti-tumor effects of extracts from various herbs and medicinal plants were measured using various in vitro and in vivo methods. The current study investigates and compare the anticancer effects of the two palm part extracts against cancer cell line in cultured cell line of AMN3, Hela and Ref. The results showed a concentration dependent inhibitory cytotoxic effects during 72 hrs of exposure for methanolic and oily crude extracts of date and leaves on AMN3, Hela and Ref cell lines. The highest significant effect of both dry date and leave methanolic extracts were achieved a

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Novotná, Barbora, Pavel Holík, Yuliya Morozova, Matej Rosa, Adéla Galandáková, and Kateřina Langová. "Evaluation of Cytotoxicity of the Dental Materials TheraCal LC, TheraCal PT, ApaCal ART and Biodentine Used in Vital Pulp Therapy: In Vitro Study." Dentistry Journal 12, no. 8 (2024): 249. http://dx.doi.org/10.3390/dj12080249.

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(1) Background: The aim of this study was to compare the cytotoxicity of selected resin-modified materials used in direct contact with the dental pulp (TheraCal LC, TheraCal PT, and ApaCal ART) with calcium silicate cement (Biodentine). (2) Methods: The mouse fibroblast Balb/3T3 cell line and the extracts of tested materials in four concentrations were used for the testing. An MTT assay was performed in three independent experiments with six replicates for each concentration of tested material. The cell viability (%) and cytotoxicity were expressed (cytotoxic effect is considered in cases wher

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Wagner, Johanna E., Janice L. Huff, William L. Rust, Karl Kingsley, and George E. Plopper. "Perillyl Alcohol Inhibits Breast Cell Migration without Affecting Cell Adhesion." Journal of Biomedicine and Biotechnology 2, no. 3 (2002): 136–40. http://dx.doi.org/10.1155/s1110724302207020.

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The monoterpene d-limonene exhibits chemotherapeutic and chemopreventive potential in breast cancer patients. D-limonene and its related compounds, perillyl alcohol and perillyl aldehyde, were chosen as candidate drugs for application in a screen for nontoxic inhibitors of cell migration. Using the nontumorigenic human breast cell line MCF-10A, we delineated the toxicity as greatest for the perillyl aldehyde, intermediate for perillyl alcohol, and least for limonene. A noncytotoxic concentration of 0.5 mmol/L perillyl alcohol inhibited the migration, while the same concentration of limonene fa

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Laila, Farida, Dedi Fardiaz, Nancy Dewi Yuliana, M. Rizal M. Damanik, and Fitriya Nur Annisa Dewi. "Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr." International Journal of Food Science 2020 (January 25, 2020): 1–12. http://dx.doi.org/10.1155/2020/9068326.

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Coleus amboinicus(Lour) (CA) has been reported to possess many pharmacological activities. In this study, evaluation of cytotoxicity using brine shrimp lethality bioassay and MTT assay using WiDr cell lines was carried out. The expression of several genes responsible for programmed cell death of the methanol extract of CA was also investigated. The morphology of the cells undergoing apoptosis was detected using Hoechst staining assay. The gene expression of BAX, BCL2, P53, Caspase 1, 7, 8, and 9 of treated samples with different concentrations (10, 15, 25 & 50 µg/ml) were measured with RT

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Ren, Kai, Yong X. Gan, Efstratios Nikolaidis, Sharaf Al Sofyani, and Lihua Zhang. "Electrolyte Concentration Effect of a Photoelectrochemical Cell Consisting of TiO2 Nanotube Anode." ISRN Materials Science 2013 (March 20, 2013): 1–7. http://dx.doi.org/10.1155/2013/682516.

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The photoelectrochemical responses of a TiO2 nanotube anode in ethylene glycol (EG), glycerol, ammonia, ethanol, urea, and Na2S electrolytes with different concentrations were investigated. The TiO2 nanotube anode was highly efficient in photoelectrocatalysis in these solutions under UV light illumination. The photocurrent density is obviously affected by the concentration change. Na2S generated the highest photocurrent density at 0, 1, and 2 V bias voltages, but its concentration does not significantly affect the photocurrent density. Urea shows high open circuit voltage at proper concentrati

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Zhao, Yu, Xiao Bin Wang, Peng Li, and Yan Ping Sun. "The Influence of Mediator Concentration on the Performance of Microbial Fuel Cell." Advanced Materials Research 512-515 (May 2012): 1520–24. http://dx.doi.org/10.4028/www.scientific.net/amr.512-515.1520.

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Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), power density and anode potential are used to characterize the mediator microbial fuel cell at different methylene blue (MB) concentrations. At lower MB concentration between 9.98×10-3 mmol/L and 1.66×10-1 mmol/L, the increased power density is enabled by using high mediator concentrations. Higher peak power density of 159.6 mw/m2 is observed compared with the peak power density of 36.0 mw/m2. But MB at too high concentration is disadvantageous to the perform of MFC. At the MB concentration of 2.50×10-1 mmol/L, the peak pow

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Ściskalska, Milena, Grzegorz Marek, Zygmunt Grzebieniak, and Halina Milnerowicz. "Resistin as a Prooxidant Factor and Predictor of Endothelium Damage in Patients with Mild Acute Pancreatitis Exposed to Tobacco Smoke Xenobiotics." Mediators of Inflammation 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/3039765.

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Objectives. The study was aimed to assess the influence of tobacco smoke exposure on the intensity of inflammation measured by IL-6, α1-antitripsin (AAT) and α1-acid glycoprotein (AGP) concentrations, and Cd level and oxidative stress intensity measured by advanced oxidation protein product (AOPP) concentration in the blood of healthy subjects and AP patients during hospitalization. Endothelin-1 (ET-1) and resistin concentrations, markers of endothelium injury, were determined. Results. An increased IL-6 concentration in healthy smokers compared to nonsmokers and AP patients compared to contro

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Dinh Lam, Nguyen, Le Thuy Trang, Nguyen Thi Mui, Pham Van Vinh, Vuong Van Cuong, and Nguyen Van Hung. "INFLUENCES OF Sn DOPING CONCENTRATION ON CHARACTERISTICS OF ZnO FILMS FOR SOLAR CELL APPLICATIONS." Journal of Science, Natural Science 60, no. 7 (2015): 41–46. http://dx.doi.org/10.18173/2354-1059.2015-0030.

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Alyousif, Nassir Abdullah, Yasin Y. Y. Al-luaibi, and Wijdan H. Al-tamimi. "Evaluation of bacterial biosurfactant activities as an anticancer and antibiofilm agent." Journal of Applied and Natural Science 17, no. 1 (2025): 313–19. https://doi.org/10.31018/jans.v17i1.6372.

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Rhamnolipids are glycolipid biosurfactants produced by Pseudomonas sp. that can be applied in many fields, such as medicine, pharmaceuticals, cosmetics and food processing. The rhamnolipid utilized in the present study was produced from Pseudomonas aeruginosa which was isolated from hydrocarbon-contaminated soil. Different rhamnolipid concentrations were evaluated as anticancer agents against cancer cell lines, including the Hela cell line and the L20B cell line, and as antibiofilm agents against four pathogenic bacteria, including Escherichia coli, Bacillus cereus, Staphylococcus aureus and K

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Arjmandi, Hamidreza, Kajsa P. Kanebratt, Liisa Vilén, Peter Gennemark, and Adam Noel. "3D cell aggregates amplify diffusion signals." PLOS ONE 19, no. 9 (2024): e0310109. http://dx.doi.org/10.1371/journal.pone.0310109.

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Biophysical models can predict the behavior of cell cultures including 3D cell aggregates (3DCAs), thereby reducing the need for costly and time-consuming experiments. Specifically, mass transfer models enable studying the transport of nutrients, oxygen, signaling molecules, and drugs in 3DCA. These models require the defining of boundary conditions (BC) between the 3DCA and surrounding medium. However, accurately modeling the BC that relates the inner and outer boundary concentrations at the border between the 3DCA and the medium remains a challenge that this paper addresses using both theore

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Anand, Mahek, Bence Lázár, Roland Tóth, et al. "Enhancement of chicken primordial germ cell in vitro maintenance using an automated cell image analyser." Acta Veterinaria Hungarica 66, no. 4 (2018): 518–29. http://dx.doi.org/10.1556/004.2018.046.

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Primordial germ cells (PGCs) were isolated from blood samples of chicken embryos. We established four PGC lines: two males (FS-ZZ-101, GFP-ZZ-4ZP) and two females (FS-ZW-111, GFP-ZW-5ZP). We could not detect a significant difference in the marker expression profile, but there was a remarkable difference between the proliferation rates of these PGC lines. We monitored the number of PGCs throughout a three-day period using a high-content screening cell imaging and analysing system (HCS). We compared three different initial cell concentrations in the wells: ~1000 cells (1×, ~4000 (4× and ~8000 (8

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Sanz del Pozo, Mónica, Cristina Plaza Alonso, Álvaro Linacero Gracia, et al. "Prognostic Implications of Serum Carbonic Anhydrase IX in Clear Cell Renal Cell Carcinoma." Journal of Urologic Oncology 23, no. 1 (2025): 54–62. https://doi.org/10.22465/juo.255000060003.

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Purpose: The present study aimed at determining the expression of carbonic anhydrase IX (CAIX) in tissue and serum of patients with clear cell renal cell carcinoma (ccRCC) and its relationship with clinical-pathological parameters; assessing CAIX as a marker of progression and survival; and studying the relationship of CAIX concentration in serum before and after surgical intervention.Materials and Methods: Immunohistochemistry was used to assess the expression of CAIX in tumor and adjacent healthy renal tissues. The concentration of CAIX in serum was determined using commercial enzyme-linked

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Nakajima, Naoki, and Yoshito Ikada. "Effect of Solution Osmotic Pressure on Cell Fusion by Poly(Ethylene Glycol)." Journal of Bioactive and Compatible Polymers 10, no. 1 (1995): 14–27. http://dx.doi.org/10.1177/088391159501000103.

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Effects of the osmotic pressure of culture medium on the membrane fusion of L929 cells in the monolayer state were investigated using polyethylene glycol) (PEG) with the molecular weight of 3,000 at various concentrations at phosphate buffer saline (PBS). Cell incubation for fusion was performed via three stages; (1) incubation before PEG treatment (preincubation), (2) incubation in the presence of PEG (PEG incubation), and (3) incubation after PEG treatment (postincubation). The PBS concentrations half that of a isotonic solution in the pre- and postincubation stages significantly accelerated

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Cheng, Ka Yu, Caroline C. Rubina Acuña, Naomi J. Boxall, et al. "Effect of Initial Cell Concentration on Bio-Oxidation of Pyrite before Gold Cyanidation." Minerals 11, no. 8 (2021): 834. http://dx.doi.org/10.3390/min11080834.

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Bio-oxidation of refractory sulfidic gold minerals has been applied at the commercial scale as a pre-treatment to improve gold yields and reduce chemical consumption during gold cyanidation. In this study, the effect of initial cell concentration on the oxidation of pyritic gold ore was evaluated with four aerated bioreactors at 30 °C with 10% pulp density and pH maintained at 1.4 with NaOH. Results of NaOH consumption and changes in soluble Fe and S concentrations indicated that increasing the initial cell concentration from 2.3 × 107 to 2.3 × 1010 cells mL−1 enhanced pyrite oxidation during

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Siddiqui, MSI, R. Parvin, M. Giasuddin, SMZH Chowdhury, MR Islam, and EH Chowdhury. "The effect of different concentrations of Dimethyl sulfoxide (DMSO) and glycerol as cryoprotectant in preserving Vero cells." Bangladesh Veterinarian 33, no. 1 (2017): 1–7. http://dx.doi.org/10.3329/bvet.v33i1.33307.

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Different concentrations of dimethyl sulfoxide (DMSO) and glycerol were used for cryopreservation of Vero cells. After total cell count Vero cells were preserved in liquid nitrogen. Two frozen stocks were made simultaneously from the cell suspensions of same concentrations using DMSO or glycerol at concentrations of 2.5%, 5%, 10% and 15%. After six months of cryopreservation both frozen stocks were used providing same nutrients and environment for the viability of the Vero cells. The cell viability analysis was performed immediately after thawing by Trypan Blue Exclusion Test. Both cryoprotect

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Khayoon, Hussein A. "Cytotoxic Effect of Resveratrol on Colorectal Cancer Cell Line." Iraqi Journal of Veterinary Medicine 44, no. 1 (2020): 68–74. http://dx.doi.org/10.30539/ijvm.v44i1.939.

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This study aimed to examine the cytotoxic effect of resveratrol as an anticancer in human colorectal cancer (HRT) cell line by assessment of its half-maximal inhibitory concentration (IC50) and its ability to inhibit the growth of these cancerous cells. Resveratrol inhibited the proliferation of HRT cell lines when used at different increased concentrations in this study (25, 50, 100, 200, 300) μmol respectively. These increased concentrations of resveratrol caused a corresponding significant inhibition in the growth percentage of the tested cancerous cell line (13%, 31.33%, 53.66%, 63.66 %, a

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Maclay, Tyler, Joseph Vacca, Casey McComas, Alfredo Castro, Melinda Day, and Kevin Mills. "CYT01B, a Novel RAD51 Inhibitor, Act Synergistically with Both Targeted and Chemotherapeutic Anti-Cancer Agents." Blood 132, Supplement 1 (2018): 3963. http://dx.doi.org/10.1182/blood-2018-99-119373.

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Abstract We have developed CYT01B, a novel RAD51 inhibitor, that sensitizes cells to Activation Induced Cytidine Deaminase (AID) activity. In cancer cells, AID causes significant genotoxic stress through DNA replication fork damage, creating a dependency upon the homologous recombination repair factor, RAD51, for survival. CYT01B acts by destabilizing RAD51 focus formation, leading to its premature nuclear export and degradation. We have shown CYT01B to be effective in AID expressing cells, however, we had yet to address if inhibition of RAD51 could act as a sensitizer to current therapeutics.

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Stavrianakou, Sotiria, Georgios Liakopoulos, Evangelos Karvonis, Evangelia Resta, and George Karabourniotis. "Research note: Low-boron acclimation induces uptake of boric acid against a concentration gradient in root cells of Olea europaea." Functional Plant Biology 33, no. 2 (2006): 189. http://dx.doi.org/10.1071/fp05097.

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Low concentrations of boron (B) in the external medium can induce uptake mechanisms whereby plants can develop a concentration gradient for B against the external medium. These mechanisms seem to be widespread among herbaceous species. In this study, olive (Olea europaea L.) plants were acclimated to either high (23 μm; controls) or low (0.5 μm; low-B plants) concentrations of B for 45 d, in a hydroponic culture. Afterwards, a 7-h uptake experiment was conducted by transferring plants of both groups to a series of nutrient solutions with B concentrations ranging from 0.5 to 23 μm. Analysis of

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Cheimarios, Nikolaos, Barbara Pem, Andreas Tsoumanis, et al. "An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform." Nanomaterials 12, no. 22 (2022): 3935. http://dx.doi.org/10.3390/nano12223935.

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A freely available “in vitro dosimetry” web application is presented enabling users to predict the concentration of nanomaterials reaching the cell surface, and therefore available for attachment and internalization, from initial dispersion concentrations. The web application is based on the distorted grid (DG) model for the dispersion of engineered nanoparticles (NPs) in culture medium used for in vitro cellular experiments, in accordance with previously published protocols for cellular dosimetry determination. A series of in vitro experiments for six different NPs, with Ag and Au cores, are

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Bryzgunova, O. E., S. N. Tamkovich, A. V. Cherepanova, et al. "Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients." Acta Naturae 7, no. 2 (2015): 115–18. http://dx.doi.org/10.32607/20758251-2015-7-2-115-118.

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A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total poo

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Canché-Collí, César, Felipe Barahona, Luis A. Medina-Medina, and Azucena Canto. "The effect of sugar concentration on yeast growth associated with floral nectar and honey." Scientia Fungorum 52 (September 8, 2021): e1288. http://dx.doi.org/10.33885/sf.2021.52.1288.

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Background: Floral nectar and honey vary in sugar concentration, from low concentration in nectar to high concentration in honey. Variation in sugar concentration is a gradient that determines yeast growth and can lead to its ecological niche specialization. Objective: Evaluate the effect of a sugar concentration gradient on the growth kinetics and cell size of yeasts isolated from the floral nectar and honey of Melipona beecheii. Methods: Four strains identified as Metschnikowia koreensis and Sympodiomycopsis paphiopedili, isolated from floral nectar, and Starmerella apicola and Starmerella a

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Zakariah, Nor Azalina, Norazah Abd Rahman, and Noor Amelina Abdul Rahim. "Chlorella sorokiniana: Effect of Nitrate Replete Concentration on Biomass Yield, Cell and Nitrate Concentration." Key Engineering Materials 797 (March 2019): 365–72. http://dx.doi.org/10.4028/www.scientific.net/kem.797.365.

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Effect of various nitrate replete concentrations have been investigated in terms of biomass yield, cell and nitrate concentrations. Media used in this study is Bold’s basal medium which contains nitrate that act as nutrient. Its concentrations have been varied for obtaining the high biomass yield. The nitrate concentrations used were 30 mg nitrate/L, 35 mg nitrate/L, 40 mg nitrate/L, 45 mg nitrate/L and 50 mg nitrate/L) with 30 mg nitrate/L as a control. Microalgae Chlorella sp. is cultured and aerated in a Schott bottle with presents of light. Results showed that the best concentration to obt

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Ishikawa, Nilton Massuo, Maria José Tavares Ranzani-Paiva, Julio Vicente Lombardi, and Cláudia Maris Ferreira. "Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury." Brazilian Archives of Biology and Technology 50, no. 4 (2007): 619–26. http://dx.doi.org/10.1590/s1516-89132007000400007.

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Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglo

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