Relevant bibliographies by topics / Concordances coptes / Journal articles
To see the other types of publications on this topic, follow the link: Concordances coptes.

Journal articles on the topic 'Concordances coptes'

Author: Grafiati
Published: 4 June 2021
Last updated: 28 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Concordances coptes.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Vinayagamoorthy, Dilanthi, Jennifer Walsh, Kierra Gipson, et al. "Detecting EGFR mutations (L858R, T790M) using allele specific multiplex sequencing: A comparison with Pyrosequencing and TruSeq." Journal of Solid Tumors 10, no. 1 (2020): 19. http://dx.doi.org/10.5430/jst.v10n1p19.

Full text
Abstract:
We are presenting an evaluation of Allele Specific Multiplex Sequencing (ASMS) to detect two EGFR somatic mutations (L858R, T790M). Late stage lung cancer samples were tested for both EGFR mutations and were compared to either pyrosequencing or TruSeq. The analytical lower limit of detection (LLOD) for the ASMS-L858R assay was found to be 36 copies, and 72 copies for the ASMS-T790M assay. The forty-one FFPE samples that were tested for T790M showed 100% concordance with the respective comparative method. The forty-five FFPE samples tested previously by Truseq for L858R showed 100% concordance
APA, Harvard, Vancouver, ISO, and other styles
2

Coyle, J. Kevin. "Concordance des Textes de Nag Hammadi. Le codex VI Pierre Cherix Coll. «Bibliothèque copte de Nag Hammadi — Concordances», 2 Sainte-Foy, Les Presses de l'Université Laval, 1993. 495 p." Studies in Religion/Sciences Religieuses 23, no. 3 (1994): 377–78. http://dx.doi.org/10.1177/000842989402300320.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Mor, Orna, Yael Gozlan, Marina Wax, et al. "Evaluation of the RealTime HIV-1, Xpert HIV-1, and Aptima HIV-1 Quant Dx Assays in Comparison to the NucliSens EasyQ HIV-1 v2.0 Assay for Quantification of HIV-1 Viral Load." Journal of Clinical Microbiology 53, no. 11 (2015): 3458–65. http://dx.doi.org/10.1128/jcm.01806-15.

Full text
Abstract:
HIV-1 RNA monitoring, both before and during antiretroviral therapy, is an integral part of HIV management worldwide. Measurements of HIV-1 viral loads are expected to assess the copy numbers of all common HIV-1 subtypes accurately and to be equally sensitive at different viral loads. In this study, we compared for the first time the performance of the NucliSens v2.0, RealTime HIV-1, Aptima HIV-1 Quant Dx, and Xpert HIV-1 viral load assays. Plasma samples (n= 404) were selected on the basis of their NucliSens v2.0 viral load results and HIV-1 subtypes. Concordance, linear regression, and Bland
APA, Harvard, Vancouver, ISO, and other styles
4

Yoo, In Young, Hyun Soo Seok, Joo An Kwon, et al. "Evaluation of the BioFire® FilmArray® Pneumonia Panel with Conventional Bacterial Culture in Conjunction with Leukocyte Esterase Test." Diagnostics 13, no. 11 (2023): 1847. http://dx.doi.org/10.3390/diagnostics13111847.

Full text
Abstract:
We evaluated the performance of the BioFire® FilmArray® Pneumonia panel (PN-panel) in detecting bacterial pathogens by comparing it to cultures and to the usefulness of the leukocyte esterase (LE) urine strip test. Between January and June 2022, a total of 67 sputum specimens were obtained from community-acquired pneumonia patients. The PN-panel and LE test were performed simultaneously with conventional cultures. The pathogen detection rates of the PN-panel and culture were 40/67 (59.7%) and 25/67 (37.3%), respectively. The concordance rate between the PN-panel and culture was high (76.9%) wh
APA, Harvard, Vancouver, ISO, and other styles
5

Fabeni, Lavinia, Giulia Berno, Valentina Svicher, et al. "Genotypic Tropism Testing in HIV-1 Proviral DNA Can Provide Useful Information at Low-Level Viremia." Journal of Clinical Microbiology 53, no. 9 (2015): 2935–41. http://dx.doi.org/10.1128/jcm.00893-15.

Full text
Abstract:
The possibility of performing genotypic tropism testing (GTT) with proviral DNA (pvDNA) even during suppressed viremia would facilitate the use of CCR5 inhibitors as part of switching, simplification, or intensification strategies. Thus, we aimed to evaluate the tropism concordance between plasma RNA and pvDNA samples and to assess which factors could affect possible discrepancies between the two compartments. GTT was performed using both plasma RNA and pvDNA from 55 sample pairs from drug-experienced patients. Potential differences between the two compartments were evaluated by analyzing core
APA, Harvard, Vancouver, ISO, and other styles
6

Gabrielli, Liliana, Miriam Tomaiuolo, Isabella Banchini, et al. "Performance Evaluation of Multiplex Molecular Syndromic Panel vs. Singleplex PCR for Diagnosis of Acute Central Nervous System Infections." Microorganisms 13, no. 4 (2025): 892. https://doi.org/10.3390/microorganisms13040892.

Full text
Abstract:
Acute central nervous system (CNS) infections, such as meningitis and encephalitis, represent medical emergencies that require rapid identification of the causative pathogen to guide appropriate therapeutic interventions. The QIAstat-Dx® Meningitis/Encephalitis (QIA/ME) is a molecular syndromic panel that enables the simultaneous detection of multiple pathogens and provides the visualization of cycle threshold (Ct) values, offering rapid results for prompt clinical management. This study retrospectively tested, with the QIA/ME panel, 170 cerebrospinal fluid (CSF) samples from patients with CNS
APA, Harvard, Vancouver, ISO, and other styles
7

Iribarnegaray, Victoria, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, and Rodrigo Puentes. "Droplet Digital PCR Enhances Sensitivity of Canine Distemper Virus Detection." Viruses 16, no. 11 (2024): 1720. http://dx.doi.org/10.3390/v16111720.

Full text
Abstract:
Canine distemper virus (CDV) poses a substantial threat to diverse carnivorans, leading to systemic and often fatal diseases. Accurate and prompt diagnosis is paramount for effective management and curbing further transmission. This study evaluates the diagnostic performance of droplet digital PCR (ddPCR) in comparison to conventional reverse-transcription (RT-PCR) and quantitative reverse-transcription real-time PCR (RT-qPCR). Seventy-six clinical samples were collected from dogs with CDV symptoms diagnosed by specialized veterinarians, and sixteen samples from apparently healthy individuals.
APA, Harvard, Vancouver, ISO, and other styles
8

Sinclair, Alistair, Catherine Arnold, and Neil Woodford. "Rapid Detection and Estimation by Pyrosequencing of 23S rRNA Genes with a Single Nucleotide Polymorphism Conferring Linezolid Resistance in Enterococci." Antimicrobial Agents and Chemotherapy 47, no. 11 (2003): 3620–22. http://dx.doi.org/10.1128/aac.47.11.3620-3622.2003.

Full text
Abstract:
ABSTRACT Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.
APA, Harvard, Vancouver, ISO, and other styles
9

Mellert, Hestia S., Leisa Jackson, and Gary Anthony Pestano. "Performance verification of a plasma-based PD-L1 test that reliably measures mRNA expression from patients with NCSLC." Journal of Clinical Oncology 36, no. 5_suppl (2018): 156. http://dx.doi.org/10.1200/jco.2018.36.5_suppl.156.

Full text
Abstract:
156 Background: The detection of circulating nucleic acids using non-invasive blood-draws has become highly relevant to clinical testing. In this study we report on the development of a blood-based PD-L1 test for immunotherapy selection. Methods: We have previously reported on the analytic performance of a droplet digital™ PCR (ddPCR) assay for circulating cytokeratin 19 and PD-L1. Using a variable threshold based on a logistic regression score for the blood assay and a 1% IHC (immunohistochemistry) tissue cut-off, concordance was 80% (n = 16). Positive calls for the blood-based PD-L1 assay ra
APA, Harvard, Vancouver, ISO, and other styles
10

Saravanan, Shanmugam, Selvamurthi Gomathi, Allison Delong, et al. "High discordance in blood and genital tract HIV-1 drug resistance in Indian women failing first-line therapy." Journal of Antimicrobial Chemotherapy 73, no. 8 (2018): 2152–61. http://dx.doi.org/10.1093/jac/dky154.

Full text
Abstract:
AbstractObjectivesExamine HIV-1 plasma viral load (PVL) and genital tract (GT) viral load (GVL) and drug resistance in India.MethodsAt the YRG Centre for AIDS Research and Education, Chennai, we tested: PVL in women on first-line ART for ≥6 months; GVL when PVL >2000 copies/mL; and plasma, genital and proviral reverse transcriptase drug resistance when GVL >2000 copies/mL. Wilcoxon rank-sum and Fisher's exact tests were used to identify failure and resistance associations. Pearson correlations were calculated to evaluate PVL–GVL associations. Inter-compartmental resistance discordance wa
APA, Harvard, Vancouver, ISO, and other styles
11

Egharevba, Jolly Osaretin, and Festus Amasikomwan Atewe. "Insecurity and the Coping Strategies of Residents in Benin Metropolis, Nigeria." Ghana Journal of Geography 16, no. 3 (2024): 108–19. http://dx.doi.org/10.4314/gjg.v16i3.11.

Full text
Abstract:
This paper examines urban insecurity and the coping strategies of residents in the Benin metropolis. The main objective of the research is to determine insecurity challenges and coping strategies of residents in the Benin metropolitan area. The research adopted the survey method. A total of 384 copies of the questionnaire were administered to the residents in the study area. However, only 367 valid copies of the questionnaire were returned. The results of the analysis revealed that armed robbery was the most common crime incident in the study area with Kendall’s Coefficient of Concordance valu
APA, Harvard, Vancouver, ISO, and other styles
12

Singh, Samir P., Hugh Salamon, Carol J. Lahti, Mehran Farid-Moyer, and Peter M. Small. "Use of Pulsed-Field Gel Electrophoresis for Molecular Epidemiologic and Population Genetic Studies ofMycobacterium tuberculosis †." Journal of Clinical Microbiology 37, no. 6 (1999): 1927–31. http://dx.doi.org/10.1128/jcm.37.6.1927-1931.1999.

Full text
Abstract:
Pulsed-field gel electrophoresis (PFGE) is a powerful molecular biology technique which has provided important insights into the epidemiology and population biology of many pathogens. However, few studies have used PFGE for the molecular epidemiology ofMycobacterium tuberculosis. A laboratory protocol was developed to determine the typeability, stability, and reproducibility of PFGE typing of M. tuberculosis. Formal data-analytical techniques were used to assess the genetic diversity elucidated by PFGE analyses using four separate restriction enzymes and by IS6110 RFLP analyses, as well as to
APA, Harvard, Vancouver, ISO, and other styles
13

Günthard, Huldrych F., Joseph K. Wong, Caroline C. Ignacio, et al. "Human Immunodeficiency Virus Replication and Genotypic Resistance in Blood and Lymph Nodes after a Year of Potent Antiretroviral Therapy." Journal of Virology 72, no. 3 (1998): 2422–28. http://dx.doi.org/10.1128/jvi.72.3.2422-2428.1998.

Full text
Abstract:
ABSTRACT Potent antiretroviral therapy can reduce human immunodeficiency virus (HIV) in plasma to levels below the limit of detection for up to 2 years, but the extent to which viral replication is suppressed is unknown. To search for ongoing viral replication in 10 patients on combination antiretroviral therapy for up to 1 year, the emergence of genotypic drug resistance across different compartments was studied and correlated with plasma viral RNA levels. In addition, lymph node (LN) mononuclear cells were assayed for the presence of multiply spliced RNA. Population sequencing of HIV-1 pol w
APA, Harvard, Vancouver, ISO, and other styles
14

Bateman, Allen C., Alexander L. Greninger, Ederlyn E. Atienza, Ajit P. Limaye, Keith R. Jerome, and Linda Cook. "Quantification of BK Virus Standards by Quantitative Real-Time PCR and Droplet Digital PCR Is Confounded by Multiple Virus Populations in the WHO BKV International Standard." Clinical Chemistry 63, no. 3 (2017): 761–69. http://dx.doi.org/10.1373/clinchem.2016.265512.

Full text
Abstract:
Abstract BACKGROUND The WHO recently released a BK virus (BKV) international standard. This study evaluated the WHO international standard and commercially available BKV standards by quantitative real-time PCR (qPCR) and droplet digital PCR (ddPCR). METHODS WHO, Exact Diagnostics, Acrometrix, and Zeptometrix BKV standards were tested by qPCR and ddPCR. Two preparations of NIST BKV clones were also tested. Nucleic acid was extracted with the Roche MP96 and MPLC, followed by quantification in duplicate. To resolve discrepancies, we sequenced the WHO and NIST materials. RESULTS Manufacturers' exp
APA, Harvard, Vancouver, ISO, and other styles
15

Milosevic, Dragana, John R. Mills, Michael B. Campion, et al. "Applying Standard Clinical Chemistry Assay Validation to Droplet Digital PCR Quantitative Liquid Biopsy Testing." Clinical Chemistry 64, no. 12 (2018): 1732–42. http://dx.doi.org/10.1373/clinchem.2018.291278.

Full text
Abstract:
Abstract BACKGROUND Droplet digital PCR (ddPCR) is an emerging technology for quantitative cell-free DNA oncology applications. However, assay performance criteria must be established in a standardized manner to harness this potential. We reasoned that standard protocols used in clinical chemistry assay validation should be able to fill this need. METHODS We validated KRAS, EGFR, and BRAF quantitative ddPCR assays based on the Clinical Laboratory Improvement Act regulations for laboratory-developed tests in clinical chemistry and the matching Clinical and Laboratory Standards Institute guideli
APA, Harvard, Vancouver, ISO, and other styles
16

Park, Bosung, Eun Jeong Won, Heungsup Sung, and Mi-Na Kim. "Performance validation of the BD MAX Enteric Parasite Panel using simulated samples in low endemic regions." Parasites, Hosts and Diseases 63, no. 1 (2025): 50–56. https://doi.org/10.3347/phd.24071.

Full text
Abstract:
Molecular diagnostics are essential for detecting intestinal parasites, but evaluating clinical samples from low endemic areas, including Korea, is challenging. We tested the performance of the BD MAX Enteric Parasite Panel in simulated samples for clinical use. Simulated samples were prepared with residual stool samples to confirm the diagnostic performance of the kits. Standard materials for Cryptosporidium parvum, Giardia lamblia, and Entamoeba histolytica were obtained for assessment. Limit of detection was determined by diluting standard materials into multiple concentrations and testing
APA, Harvard, Vancouver, ISO, and other styles
17

Mossoro-Kpinde, Christian Diamant, Ralph-Sydney Mboumba Bouassa, Mohammad-Ali Jenabian, et al. "Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification." AIDS Research and Treatment 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/7954810.

Full text
Abstract:
Objectives. We evaluated the performances of Amplix real-time PCR platform developed by Biosynex (Strasbourg, France), combining automated station extraction (Amplix station 16 Dx) and real-time PCR (Amplix NG), for quantifying plasma HIV-1 RNA by lyophilized HIV-1 RNA-based Amplix reagents targeting gag and LTR, using samples from HIV-1-infected adults from Central African Republic. Results. Amplix real-time PCR assay showed low limit of detection (28 copies/mL), across wide dynamic range (1.4–10 log copies/mL), 100% sensitivity and 99% specificity, high reproducibility, and accuracy with mea
APA, Harvard, Vancouver, ISO, and other styles
18

Janku, Filip, Afsaneh Barzi, Andrea Sartore-Bianchi, et al. "Mutation enrichment next-generation sequencing for quantitative detection of KRAS mutations in urine cell-free DNA from patients with advanced colorectal and other cancers." Journal of Clinical Oncology 35, no. 4_suppl (2017): 602. http://dx.doi.org/10.1200/jco.2017.35.4_suppl.602.

Full text
Abstract:
602 Background: Molecular testing of cell-free (cf) DNA from urine is a completely non-invasive approach for detection of actionable mutations in cancer. Methods: A quantitative mutation enrichment next-generation sequencing (NGS) urine cell-free (cf) DNA KRASG12/G13mutation test was developed and results compared to clinical testing of archival tumor tissue and research testing of plasma cfDNA from patients with advanced colorectal (n=56, 79%) and other advanced cancers (n=15, 21%). Results: The analytical sensitivity of the KRASG12/G13 cfDNA test was 0.002%-0.006% mutant copies in wild-type
APA, Harvard, Vancouver, ISO, and other styles
19

Gavito, Cory M. "“Quasi industre giardiniero”." Journal of Musicology 33, no. 4 (2016): 522–68. http://dx.doi.org/10.1525/jm.2016.33.4.522.

Full text
Abstract:
Among the roughly 150 Italian songbooks published between 1610 and 1665 with the guitar tablature known as alfabeto, about thirteen are anthologies. These anthologies often advertise the role of a compiler who has gathered together music by diverse authors. The extent to which compilers also functioned as authors and editors is not well understood. This essay considers the case of Giovanni Stefani, a compiler who, in the preface to his Scherzi amorosi of 1622, describes the anthology as a collection of his choosing that contains “varie compositioni de Virtuosi della prima classe” (various comp
APA, Harvard, Vancouver, ISO, and other styles
20

Delic, Dragan, Zorica Nesic, Jasmina Simonovic, Neda Svirtlih, Ljubisa Dokic, and Gorana Neskovic. "Chronic hepatitis C virus infection: Is there a correlation between HCV genotypes and the level of viremia?" Medical review 59, no. 5-6 (2006): 230–34. http://dx.doi.org/10.2298/mpns0606230d.

Full text
Abstract:
Introduction. Hepatitis C virus (HCV) RNA status and HCV genotypes have become extremely important for exact diagnosis, prognosis, duration of treatment and monitoring of antiviral therapy of chronic HCV infection. Material and methods. For the purpose of precise and objective assessment of virologic analyses, such as the determination of the number of virus copies and virus genotypes, 110 patients with chronic HCV infection were tested. Genotyping of HCV isolates and HCV RNA quantification were performed by using the PCR method. Genotype lb infection was verified in 49.1% of patients, genotyp
APA, Harvard, Vancouver, ISO, and other styles
21

Hall, Andrew G., Sarina Sulong, Christine Harrison, et al. "Assessment of Aneuploidy in Childhood Acute Lymphoblastic Leukaemia Using High Density Oligonucleotide Arrays." Blood 108, no. 11 (2006): 104. http://dx.doi.org/10.1182/blood.v108.11.104.104.

Full text
Abstract:
Abstract Chromosomal copy number (CN) is an important prognostic index in childhood acute lymphoblastic leukaemia (ALL). High hyperdiploidy (51–65 chromosomes) is associated with a good prognosis and near haploidy (23–29) a poor outcome. Conventional cytogenetics produces accurate estimates of CN in the majority of cases but sometimes cytogenetic fails or the morphology of chromosomes in ALL is too poor for accurate identification. We have used single nucleotide polymorphism arrays (SNPA) to determine CN in 86 childhood ALL patients and have compared the results with conventional cytogenetic a
APA, Harvard, Vancouver, ISO, and other styles
22

Lippert, Eric, François Girodon, Emma Hammond, et al. "Concordance of Assays Designed for the Quantitation of JAK2 1849G>T (V617F): A Multi-Centre Study." Blood 110, no. 11 (2007): 2529. http://dx.doi.org/10.1182/blood.v110.11.2529.2529.

Full text
Abstract:
Abstract Studies of myeloproliferative disorders (MPDs) aiming to evaluate the fraction of the clone bearing the JAK2 1849T mutation occasionally report discordant findings. One reason could be different sensitivity and accuracy of the various assays designed for the detection and quantitation of JAK2 1849G>T. We studied the concordance of 10 published JAK2 1849G>T assays. 29 samples of genomic DNA were distributed to 14 laboratories in France, USA, Australia, Germany, Holland, Italy and Switzerland for blinded assessment of JAK2 1849T levels. DNA was extracted from granulocytes
APA, Harvard, Vancouver, ISO, and other styles
23

Kanakry, Jennifer Ann, Lan L. Gellert, Yvette L. Kasamon, M. Victor Lemas, Marie Valerie Toure, and Richard F. Ambinder. "Cell-free EBV DNA in Hodgkin lymphoma." Journal of Clinical Oncology 30, no. 30_suppl (2012): 7. http://dx.doi.org/10.1200/jco.2012.30.30_suppl.7.

Full text
Abstract:
7 Background: EBV is associated with a subset of Hodgkin lymphoma (HL), identified by tissue-based techniques such as EBER in situ hybridization of tumor specimens. Recent evidence suggests that cell-free EBV DNA may be a surrogate for EBER and serve as a tumor marker in HL. Methods: EBV DNA copy number was quantitated by RT-PCR of plasma, serum, and peripheral blood mononuclear cells (PBMCs) from HL patients and compared using Pearson’s correlation. Receiver operator characteristic (ROC) curves determined thresholds for EBV DNA copy number to discriminate EBER status. Kaplan-Meier curves and
APA, Harvard, Vancouver, ISO, and other styles
24

Oscorbin, Igor P., Georgiy Yu Shevelev, Ksenia A. Pronyaeva, et al. "Detection of SARS-CoV-2 RNA by a Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Melting Curves Analysis." International Journal of Molecular Sciences 22, no. 11 (2021): 5743. http://dx.doi.org/10.3390/ijms22115743.

Full text
Abstract:
Loop-mediated isothermal amplification (LAMP) is a method of nucleic acid amplification that is more stable and resistant to DNA amplification inhibitors than conventional PCR. LAMP multiplexing with reverse transcription allows for the single-tube amplification of several RNA fragments, including an internal control sample, which provides the option of controlling all analytical steps. We developed a method of SARS-CoV-2 viral RNA detection based on multiplex reverse-transcription LAMP, with single-tube qualitative analysis of SARS-CoV-2 RNA and MS2 phage used as a control RNA. The multiplexi
APA, Harvard, Vancouver, ISO, and other styles
25

Zhang, Letian, Zhiwen Jiang, Zitong Zhou, et al. "A TaqMan Probe-Based Multiplex Real-Time PCR for Simultaneous Detection of Porcine Epidemic Diarrhea Virus Subtypes G1 and G2, and Porcine Rotavirus Groups A and C." Viruses 14, no. 8 (2022): 1819. http://dx.doi.org/10.3390/v14081819.

Full text
Abstract:
Porcine viral diarrhea diseases affect the swine industry, resulting in significant economic losses. Porcine epidemic diarrhea virus (PEDV) genotypes G1 and G2, and groups A and C of the porcine rotavirus, are major etiological agents of severe gastroenteritis and profuse diarrhea, particularly among piglets, with mortality rates of up to 100%. Based on the high prevalence rate and frequent co-infection of PEDV, RVA, and RVC, close monitoring is necessary to avoid greater economic losses. We have developed a multiplex TaqMan probe-based real-time PCR for the rapid simultaneous detection and di
APA, Harvard, Vancouver, ISO, and other styles
26

Pang, Tatiana A., та Giovanni Enrico Stary. "The Content and Concordance of the Chapters from the Manchu-Chinese Manuscript Emu Tanggû Orin Sakda-i Gisun Sarkiyan 百二老人語録 Kept in the IOM, RAS". Written Monuments of the Orient 10, № 1 (2024): 62–79. http://dx.doi.org/10.55512/wmo633237.

Full text
Abstract:
The Manchu text “The stories of one hundred and twenty old men” Emu tanggû orin sakda-i gisun sarkiyan compiled by Songyûn (Songyun 松筠) in 1790 was edited by Furentai, and then translated into Chinese by Fugiyûn (Fujun 富俊) in 1809. The text exists only in a manuscript form and was never published. Monolingual Manchu and bilingual Manchu-Chinese versions of this text are scattered all over the world. For nearly twenty years the text existed only in Manchu and had suffered edition and rearrangement of the stories’ order. That fact inspired the late Prof. Giovanni Stary to compare the available c
APA, Harvard, Vancouver, ISO, and other styles
27

Kan, Xingchi, Yue Wu, Xiyue Zhang, et al. "Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a." Transboundary and Emerging Diseases 2024 (May 28, 2024): 1–12. http://dx.doi.org/10.1155/2024/5002521.

Full text
Abstract:
The recent high incidence of Mycoplasma pneumoniae (Mp) infections has raised widespread public health concerns. Therefore, rapid and accurate diagnosis of respiratory pathogenic microbial infections is of paramount importance to provide clinicians with accurate diagnostic insights and guide clinical medication. In response to this urgent need, we developed a one-tube Palm CRISPR/Cas12a Diagnostic (PaCD) method. This method facilitates the rapid detection of Mp infections, as well as three other prevalent respiratory pathogens, Chlamydia pneumoniae (Cp), Haemophilus influenzae (Hi), and Strept
APA, Harvard, Vancouver, ISO, and other styles
28

Armenia, Daniele, Luca Carioti, Valeria Micheli, et al. "Comparison of Different HIV-1 Resistance Interpretation Tools for Next-Generation Sequencing in Italy." Viruses 16, no. 9 (2024): 1422. http://dx.doi.org/10.3390/v16091422.

Full text
Abstract:
Background: Next-generation sequencing (NGS) is gradually replacing Sanger sequencing for HIV genotypic drug resistance testing (GRT). This work evaluated the concordance among different NGS-GRT interpretation tools in a real-life setting. Methods: Routine NGS-GRT data were generated from viral RNA at 11 Italian laboratories with the AD4SEQ HIV-1 Solution v2 commercial kit. NGS results were interpreted by the SmartVir system provided by the kit and by two online tools (HyDRA Web and Stanford HIVdb). NGS-GRT was considered valid when the coverage was >100 reads (100×) at each PR/RT/IN resist
APA, Harvard, Vancouver, ISO, and other styles
29

Schäfer, Peter, Werner Tenschert, Matthias Schröter, Kai Gutensohn, and Rainer Laufs. "False-Positive Results of Plasma PCR for Cytomegalovirus DNA due to Delayed Sample Preparation." Journal of Clinical Microbiology 38, no. 9 (2000): 3249–53. http://dx.doi.org/10.1128/jcm.38.9.3249-3253.2000.

Full text
Abstract:
Positive results by cytomegalovirus (CMV) PCR of plasma are considered predictive of active CMV infection in kidney allograft recipients. To assess whether contamination with leukocyte-derived CMV DNA can distort the results, aliquots of whole-blood samples from 60 CMV immunoglobulin G-positive patients with leukocyte CMV DNAemia were stored for up to 24 h at room temperature (RT) and at 4°C before plasma preparation. Native and ultrafiltered plasma samples were tested by CMV and β-globin PCRs. Among 30 latently infected patients (negative for CMV pp65 antigens), low baseline rates (10%) and l
APA, Harvard, Vancouver, ISO, and other styles
30

de Sousa, Karoline Almeida Felix, Carolina Kymie Vasques Nonaka, Ricardo Khouri, Clarissa Araújo Gurgel Rocha, Carlos Gustavo Regis-Silva, and Bruno Solano de Freitas Souza. "Rapid Detection of SARS-CoV-2 Based on the LAMP Assay Associated with the CRISPRCas12a System." Diagnostics 13, no. 13 (2023): 2233. http://dx.doi.org/10.3390/diagnostics13132233.

Full text
Abstract:
Background: The global public health system has been severely tested by the COVID-19 pandemic. Mass testing was essential in controlling the transmission of the SARS-CoV-2; however, its implementation has encountered challenges, particularly in low-income countries. The urgent need for rapid and accurate tests for SARS-CoV-2 has proven to be extremely important. Point-of-care tests using the CRISPR system for COVID-19 have shown promise, with a reported high sensitivity and rapid detection. The performance of a CRISPR-based SARS-CoV-2 testing system was reported in this study. Methods: A total
APA, Harvard, Vancouver, ISO, and other styles
31

Harvey, Justin C., Lisa M. Cambridge, Charles W. Ellen, et al. "Analytical Validation of Cxbladder® Detect, Triage, and Monitor: Assays for Detection and Management of Urothelial Carcinoma." Diagnostics 14, no. 18 (2024): 2061. http://dx.doi.org/10.3390/diagnostics14182061.

Full text
Abstract:
Background: Cxbladder® assays are reverse transcription-quantitative polymerase chain reaction (RT-qPCR) tests incorporating five genetic biomarkers (CDK1, MDK, IGFBP5, HOXA13, and CXCR2) to provide risk stratification for urothelial carcinoma (UC) in patients with hematuria or undergoing surveillance for recurrent disease. This study evaluated the analytical validity of the Cxbladder Detect, Triage, and Monitor assays. Methods: Pre-specified acceptance criteria, including the assays’ fundamental aspects (sample and reagent stability, RNA extraction quality, RT-qPCR linearity, and analytical s
APA, Harvard, Vancouver, ISO, and other styles
32

Chen, Wenwen, Jiaying Zheng, Chang Wu, et al. "Breast Cancer Subtype Classification Using 4-Plex Droplet Digital PCR." Clinical Chemistry 65, no. 8 (2019): 1051–59. http://dx.doi.org/10.1373/clinchem.2019.302315.

Full text
Abstract:
Abstract BACKGROUND Infiltrating ductal carcinoma (IDCA) is the most common form of invasive breast cancer. Immunohistochemistry (IHC) is widely used to analyze estrogen receptor 1 (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) that can help classify the tumor to guide the medical treatment. IHC examinations require experienced pathologists to provide interpretations that are subjective, thereby lowering the reproducibility of IHC-based diagnosis. In this study, we developed a 4-plex droplet digital PCR (ddPCR) for the simultaneous and quantitative analys
APA, Harvard, Vancouver, ISO, and other styles
33

Đinh, Thu Hằng, Đăng Dũng Nguyễn, Thu Hà Hoàng та Xuân Sử Hoàng. "SO SÁNH HAI MASTER MIX PCR PROBE TRONG KỸ THUẬT REAL-TIME PCR XÁC ĐỊNH BK POLYOMAVIRUS". Tạp chí Khoa học và Công nghệ Nhiệt đới, № 36 (29 квітня 2025): 96–105. https://doi.org/10.58334/vrtc.jtst.n36.10.

Full text
Abstract:
COMPARISON OF TWO MASTER MIXES REAL-TIME POLYMERASE CHAIN REACTION PROBE FOR BK POLYOMAVIRUS DETECTIONComparison of the efficiency of probe real-time PCR master mixes for BKV detection offers additional PCR reagent options for clinical laboratory tests. In the present study, AceQ qPCR Probe Master Mix was compared with QuantiTect Probe PCR Master Mix in the real-time PCR assay targeting the VP1-BKV gene. The Rotor-Gene Q platform (Qiagen, Germany) and a 10-fold serial dilution of recombinant BKV-VP1 plasmid ranging from 2.10-1 copies/µl to 2.105 copies/µl were used to evaluate. There were diff
APA, Harvard, Vancouver, ISO, and other styles
34

Cheng, Zheng-Jiang, Li-Hua Hu, Wen-Rong Fu, and Yi-Rong Li. "Rapid quantification of hepatitis B virus DNA by direct real-time PCR from serum without DNA extraction." Journal of Medical Microbiology 56, no. 6 (2007): 766–71. http://dx.doi.org/10.1099/jmm.0.47154-0.

Full text
Abstract:
The purpose of this study was to quantify hepatitis B virus DNA by direct real-time PCR from serum without the need for DNA extraction. Crossing point (Cp) values were determined automatically using the second derivative maximum mode. Since serum samples from patients are inevitably haemolysed, lipaemic or icteric, the interference of endogenous substances from the serum in real-time PCR was evaluated. The result showed that, although serum protein quenched the intensity of fluorescence, the Cp value adopted to calculate the quantity of DNA copies remained unchanged. Importantly, real-time PCR
APA, Harvard, Vancouver, ISO, and other styles
35

Koryukov, Maksim A., Igor P. Oscorbin, Lidiya M. Novikova, et al. "A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses." International Journal of Molecular Sciences 25, no. 13 (2024): 7215. http://dx.doi.org/10.3390/ijms25137215.

Full text
Abstract:
Human adenoviruses (HAdVs) are common pathogens that are associated with a variety of diseases, including respiratory tract infections (RTIs). Without reliable, fast, and cost-effective detection methods for HAdVs, patients may be misdiagnosed and inappropriately treated. To address this problem, we have developed a multiplex loop-mediated isothermal amplification (LAMP) assay for the detection of the species Human adenovirus B (HAdV-B), Human adenovirus C (HAdV-C) and Human adenovirus E (HAdV-E) that cause RTIs. This multiplexing approach is based on the melting curve analysis of the amplicon
APA, Harvard, Vancouver, ISO, and other styles
36

Holmes, Ann E., Melinda R. Baerwald, Jeff Rodzen, Brian M. Schreier, Brian Mahardja, and Amanda J. Finger. "Evaluating environmental DNA detection of a rare fish in turbid water using field and experimental approaches." PeerJ 11 (January 2, 2024): e16453. http://dx.doi.org/10.7717/peerj.16453.

Full text
Abstract:
Detection sensitivity of aquatic species using environmental DNA (eDNA) generally decreases in turbid water but is poorly characterized. In this study, eDNA detection targeted delta smelt (Hypomesus transpacificus), a critically endangered estuarine fish associated with turbid water. eDNA sampling in the field was first paired with a trawl survey. Species-specific detection using a Taqman qPCR assay showed concordance between the methods, but a weak eDNA signal. Informed by the results of field sampling, an experiment was designed to assess how turbidity and filtration methods influence detect
APA, Harvard, Vancouver, ISO, and other styles
37

Conciatori, Valeria, Sarah Di Sopra, Elisa Franchin, et al. "Implementation of a Laboratory-Developed Test for the Diagnosis of Mycoplasma pneumoniae Using a High-Throughput Approach." Pathogens 14, no. 7 (2025): 692. https://doi.org/10.3390/pathogens14070692.

Full text
Abstract:
Mycoplasma pneumoniae is a significant causative agent of atypical pneumonia in both children and adults. Timely and accurate diagnosis is crucial for appropriate patient management. Conventional methods for detecting M. pneumoniae, such as culture and serology, exhibit several limitations regarding sensitivity, specificity, and turnaround time. In contrast, real-time PCR is considered the most reliable, rapid, and sensitive technique for the diagnosis of M. pneumoniae infection. In this study, we adapted and validated an in-house real-time PCR assay for use on the fully automated Panther Fusi
APA, Harvard, Vancouver, ISO, and other styles
38

Vodicka, Josef, Martin Pesta, Vlastimil Kulda, et al. "Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure." Cells 9, no. 12 (2020): 2611. http://dx.doi.org/10.3390/cells9122611.

Full text
Abstract:
The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examin
APA, Harvard, Vancouver, ISO, and other styles
39

Damm-Welk, Christine, Federica Lovisa, Giorgia Contarini, et al. "Quantification of Minimal Disease by Digital PCR in ALK-Positive Anaplastic Large Cell Lymphoma: A Step towards Risk Stratification in International Trials?" Cancers 14, no. 7 (2022): 1703. http://dx.doi.org/10.3390/cancers14071703.

Full text
Abstract:
Minimal disseminated and residual disease (MDD/MRD) analyzed by qualitative PCR for NPM-ALK fusion transcripts are validated prognostic factors in pediatric ALK-positive anaplastic large cell lymphoma (ALCL). Although potentially promising, MDD quantification by quantitative real-time PCR in international trials is technically challenging. Quantification of early MRD might further improve risk stratification. We aimed to assess droplet digital PCR for quantification of minimal disease in an inter-laboratory setting in a large cohort of 208 uniformly treated ALCL patients. Inter-laboratory qual
APA, Harvard, Vancouver, ISO, and other styles
40

SoRelle, Jeffrey A., Ithiel Frame, Alejandra Falcon, et al. "Clinical Validation of a SARS-CoV-2 Real-Time Reverse Transcription PCR Assay Targeting the Nucleocapsid Gene." Journal of Applied Laboratory Medicine 5, no. 5 (2020): 889–96. http://dx.doi.org/10.1093/jalm/jfaa089.

Full text
Abstract:
Abstract Background Detection of SARS-CoV-2 viral RNA is important for the diagnosis and management of COVID-19. Methods We present a clinical validation of a reverse transcription PCR (RT-PCR) assay for the SARS-CoV-2 nucleocapsid (N1) gene. Off-board lysis on an automated nucleic acid extraction system was optimized with endemic coronaviruses (OC43 and NL63). Genomic RNA and SARS-CoV-2 RNA in a recombinant viral protein coat were used as control materials and compared for recovery from nucleic acid extraction. Results Nucleic acid extraction showed decreased recovery of endemic Coronavirus i
APA, Harvard, Vancouver, ISO, and other styles
41

Myint, Lay, Koya Ariyoshi, Hua Yan, et al. "Mutagenically Separated PCR Assay for Rapid Detection of M41L and K70R Zidovudine Resistance Mutations in CRF01_AE (Subtype E) Human Immunodeficiency Virus Type 1." Antimicrobial Agents and Chemotherapy 46, no. 12 (2002): 3861–68. http://dx.doi.org/10.1128/aac.46.12.3861-3868.2002.

Full text
Abstract:
ABSTRACT A rapid zidovudine (ZDV) resistance genotypic assay was developed based on the mutagenically separated PCR (MS-PCR) technique to detect two ZDV-resistant mutations, M41L and K70R in CRF01_AE (subtype E). Endpoint dilution analysis revealed that the newly constructed MS-PCR assay could successfully detect three to nine copies of human immunodeficiency virus type 1 template RNA. The test against wild-type and mutant template mixtures in different ratios demonstrated that the assay could detect 10% minor population, at least. Fifty-one subtype E clinical samples were analyzed by the newl
APA, Harvard, Vancouver, ISO, and other styles
42

Wang, De Guo. "Rapid Detection of Mycobacterium tuberculosis Complex by Loop-Mediated Isothermal Amplification Combined with Chemosensor." Advanced Materials Research 749 (August 2013): 449–52. http://dx.doi.org/10.4028/www.scientific.net/amr.749.449.

Full text
Abstract:
Loop-mediated isothermal amplification (LAMP) allowed rapid amplification of nucleic acids under isothermal conditions. It can be combined with a chemosensor for much more efficient, field-friendly detection of Mycobacterium tuberculosis complex. In this report, LAMP was performed at 65 °C for 10 min, followed by a rapid reaction of DNA amplification by-product, pyrophosphate ion, with chemosensor resulted in red disappearance. The detection limit of Mycobacterium tuberculosis complex by LAMP-Chemosensor was 3-5 copies, and the total assay time including 10 min for rapid DNA extraction was app
APA, Harvard, Vancouver, ISO, and other styles
43

A Castaneda, Carlos, Miluska Castillo, Joselyn Sanchez, et al. "Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR." Future Microbiology 15, no. 12 (2020): 1131–37. http://dx.doi.org/10.2217/fmb-2019-0280.

Full text
Abstract:
Aim: Helicobacter pylori is usually detected based on hematoxylin–eosin (H–E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H–E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H–E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detecti
APA, Harvard, Vancouver, ISO, and other styles
44

Saito, Ryoichi, Yoshiki Misawa, Kyoji Moriya, Kazuhiko Koike, Kimiko Ubukata, and Noboru Okamura. "Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae." Journal of Medical Microbiology 54, no. 11 (2005): 1037–41. http://dx.doi.org/10.1099/jmm.0.46071-0.

Full text
Abstract:
A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Mycoplasma pneumoniae was developed and evaluated. The assay specifically amplified only M. pneumoniae sequences, and no cross-reactivity was observed for other Mycoplasma species or respiratory bacterial species. The detection limit for this assay was found to be 2 × 102 copies, corresponding to 2–20 colour changing units of M. pneumoniae in 1 h, as observed in a real-time turbidimeter and electrophoretic analysis. The accuracy of the LAMP reaction was confirmed by restriction endonuclease analysis as well as dir
APA, Harvard, Vancouver, ISO, and other styles
45

Mohd Nor, Nor Arifah, Wardatul Akmam Din, Irma Wani Othman, and Norazah Mohd Suki. "Comparing Useful Words in Second Language Adult Learners’ Writings with BNC Wordlist: A Prototype Approach." Labuan e-Journal of Muamalat and Society (LJMS) 8 (June 30, 2014): 50–57. http://dx.doi.org/10.51200/ljms.v8i.3018.

Full text
Abstract:
This research focusses on the preliminary investigation of vocabulary knowledge on teachers undergoing the Teacher Graduate Programme (PPG) in Universiti Malaysia Sabah (UMS). By conducting this research it is possible to determine whether these teachers are familiar with the same set of words listed in BNC therefore have sufficient vocabulary knowledge which they most probably impart to their students. Forty-four adult studentswer. e asked to write an English essay based on the topic given by the teacher. Soft copies of their essays were compiled and converted to .txt files which were then tr
APA, Harvard, Vancouver, ISO, and other styles
46

Li, Renfeng, Xiangqin Tian, Wenyan Cao, et al. "Development of a Paper-Based Microfluidic Chip for Point-of-Care Detection of PEDV." Veterinary Sciences 12, no. 5 (2025): 427. https://doi.org/10.3390/vetsci12050427.

Full text
Abstract:
PEDV poses a significant threat to the global swine industry, necessitating rapid and accurate diagnostic methods for effective disease management. In this study, we developed a foldable, easy-to-use paper-based microfluidic analytical device (μPAD) for on-site detection of PEDV. The device seamlessly integrates paper-based nucleic acid enrichment, LAMP reaction, and visual lateral flow detection into a single platform. Key parameters, including nucleic acid extraction protocols, chromatographic channel configurations, colorimetric indicators, and reaction temperature and duration, were system
APA, Harvard, Vancouver, ISO, and other styles
47

Lv, Lindan, Hao Mu, Shaomei Li, et al. "Establishment of a One–Pot RAA–CRISPR/Cas13a Assay-Based TGEV S Gene Detection." Veterinary Sciences 12, no. 5 (2025): 464. https://doi.org/10.3390/vetsci12050464.

Full text
Abstract:
Porcine transmissible gastroenteritis virus (TGEV) is a highly contagious pathogen causing severe diarrhea in pigs, particularly piglets, leading to significant economic losses. Distinguishing TGEV from the genetically similar porcine respiratory coronavirus (PRCV) remains challenging due to their high genomic homology. In this study, we developed a one–pot assay combining recombinase-aided amplification (RAA) and CRISPR/Cas13a technology, targeting the TGEV S gene. This method was optimized for sensitivity and specificity, with orthogonal tests determining the optimal reagent concentrations.
APA, Harvard, Vancouver, ISO, and other styles
48

Curti, Lucía Ana, Ivana Primost, Sofia Valla, et al. "Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2." Viruses 13, no. 3 (2021): 420. http://dx.doi.org/10.3390/v13030420.

Full text
Abstract:
We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the
APA, Harvard, Vancouver, ISO, and other styles
49

Jones, Les, Abhijeet Bakre, Hemant Naikare, et al. "Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabs." PLOS ONE 16, no. 9 (2021): e0257563. http://dx.doi.org/10.1371/journal.pone.0257563.

Full text
Abstract:
The COVID-19 pandemic caused by the SARS-CoV-2 is a serious health threat causing worldwide morbidity and mortality. Real-time reverse transcription PCR (RT-qPCR) is currently the standard for SARS-CoV-2 detection. Although various nucleic acid-based assays have been developed to aid the detection of SARS-CoV-2 from COVID-19 patient samples, the objective of this study was to develop a diagnostic test that can be completed in 30 minutes without having to isolate RNA from the samples. Here, we present an RNA amplification detection method performed using reverse transcription loop-mediated isot
APA, Harvard, Vancouver, ISO, and other styles
50

Lapointe, H. R., W. Dong, G. Q. Lee, et al. "HIV Drug Resistance Testing by High-Multiplex “Wide” Sequencing on the MiSeq Instrument." Antimicrobial Agents and Chemotherapy 59, no. 11 (2015): 6824–33. http://dx.doi.org/10.1128/aac.01490-15.

Full text
Abstract:
ABSTRACTLimited access to HIV drug resistance testing in low- and middle-income countries impedes clinical decision-making at the individual patient level. An efficient protocol to address this issue must be established to minimize negative therapeutic outcomes for HIV-1-infected individuals in such settings. This is an observational study to ascertain the potential of newer genomic sequencing platforms, such as the Illumina MiSeq instrument, to provide accurate HIV drug resistance genotypes for hundreds of samples simultaneously. Plasma samples were collected from Canadian patients during rou
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography