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Journal articles on the topic 'Confocal microscopy'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

J. H., Youngblom, Wilkinson J., and Youngblom J.J. "Telepresence Confocal Microscopy." Microscopy and Microanalysis 6, S2 (2000): 1164–65. http://dx.doi.org/10.1017/s1431927600038319.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments. While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar ca

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Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Telepresence Confocal Microscopy." Microscopy Today 8, no. 10 (2000): 20–21. http://dx.doi.org/10.1017/s1551929500054146.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments, While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar ca

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Yin, Siyuan, Shibao Wu, Zhanming Li, et al. "Photonic timestamped confocal microscopy." Advanced Imaging 1, no. 2 (2024): 021005. http://dx.doi.org/10.3788/ai.2024.10011.

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4

Jester, J. V., H. D. Cavanagh, and M. A. Lemp. "In vivo confocal imaging of the eye using tandem scanning confocal microscopy (TSCM)." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 56–57. http://dx.doi.org/10.1017/s0424820100102365.

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New developments in optical microscopy involving confocal imaging are now becoming available which dramatically increase resolution, contrast and depth of focus by optically sectioning through structures. The transparency of the anterior ocular structures, cornea and lens, make microscopic visualization and optical sectioning of the living intact eye an interesting possibility. Of the confocal microscopes available, the Tandem Scanning Reflected Light Microscope (referred to here as the Tandem Scanning Confocal Microscope), developed by Professors Petran and Hadravsky at Charles University in

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Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Confocal Laser Scanning Microscopy By Remote Access." Microscopy Today 7, no. 7 (1999): 32–33. http://dx.doi.org/10.1017/s1551929500064798.

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In recent years there have been a growing number of facilities interested in developing remote access capabilities to a variety of microscopy systems. While certain types of microscopes, such as electron microscopes and scanning probe microscopes have been well established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal microscope.At California State University, home to the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote acce

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Jason Kirk. "Beyond the Hype - Is 2-Photon Microscopy Right for You?" Microscopy Today 11, no. 2 (2003): 26–29. http://dx.doi.org/10.1017/s1551929500052469.

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Confocal microscopes have come a long way in the past decade. Not only are they more stable and easier to use than ever before, but their cost has dropped enough that multi-user facilities are finding competition from individual labs using the new breed of "personal" confocals. In fact it has, in some cases, become the de facto standard for fluorescence imaging regardless of whether the user actually has requirements for it or not.But, researchers always have an ear out for something better. Enter 2-photon microscopy (2PLSM). The “bigger & badder” cousin of the confocal microscope has beco

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Stefani, Caroline, Adam Lacy-Hulbert, and Thomas Skillman. "ConfocalVR: Immersive Visualization for Confocal Microscopy." Journal of Molecular Biology 430, no. 21 (2018): 4028–35. http://dx.doi.org/10.1016/j.jmb.2018.06.035.

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8

Govil, Anurag, David M. Pallister, and Michael D. Morris. "Three-Dimensional Digital Confocal Raman Microscopy." Applied Spectroscopy 47, no. 1 (1993): 75–79. http://dx.doi.org/10.1366/0003702934048497.

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We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging.

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9

Chapman, George B., and T. Wilson. "Confocal Microscopy." Transactions of the American Microscopical Society 110, no. 2 (1991): 194. http://dx.doi.org/10.2307/3226760.

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10

Wilson, T., and Barry R. Masters. "Confocal microscopy." Applied Optics 33, no. 4 (1994): 565. http://dx.doi.org/10.1364/ao.33.000565.

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11

Beuerman, Roger W. "Confocal Microscopy." Cornea 14, no. 1 (1995): 1???2. http://dx.doi.org/10.1097/00003226-199501000-00001.

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12

Lichtman, Jeff W. "Confocal Microscopy." Scientific American 271, no. 2 (1994): 40–45. http://dx.doi.org/10.1038/scientificamerican0894-40.

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13

Luck, B., K. Carlson, T. Collier, and Kung-Bin Sung. "Confocal microscopy." IEEE Potentials 23, no. 1 (2004): 14–17. http://dx.doi.org/10.1109/mp.2004.1266933.

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14

Grant-Kels, Jane M., Giovanni Pellacani, and Caterina Longo. "Confocal Microscopy." Dermatologic Clinics 34, no. 4 (2016): i. http://dx.doi.org/10.1016/s0733-8635(16)30089-4.

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15

Chatterjee, Samrat, and Deesphikha Agrawal. "Confocal Microscopy." Ophthalmology 119, no. 2 (2012): 428–29. http://dx.doi.org/10.1016/j.ophtha.2011.10.027.

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16

Hendee, William R. "Confocal Microscopy." Academic Radiology 9, no. 5 (2002): 503. http://dx.doi.org/10.1016/s1076-6332(03)80325-2.

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17

Kaufman, Stephen C., David C. Musch, Michael W. Belin, et al. "Confocal microscopy." Ophthalmology 111, no. 2 (2004): 396–406. http://dx.doi.org/10.1016/j.ophtha.2003.12.002.

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18

Pellacani, G. "Confocal microscopy." Melanoma Research 20 (June 2010): e9-e10. http://dx.doi.org/10.1097/01.cmr.0000382763.16366.d1.

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19

Volkov, I. A., N. V. Frigo, L. F. Znamenskaya, and O. R. Katunina. "Application of Confocal Laser Scanning Microscopy in Biology and Medicine." Vestnik dermatologii i venerologii 90, no. 1 (2014): 17–24. http://dx.doi.org/10.25208/0042-4609-2014-90-1-17-24.

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Fluorescence confocal laser scanning microscopy and reflectance confocal laser scanning microscopy are up-to-date highend study methods. Confocal microscopy is used in cell biology and medicine. By using confocal microscopy, it is possible to study bioplasts and localization of protein molecules and other compounds relative to cell or tissue structures, and to monitor dynamic cell processes. Confocal microscopes enable layer-by-layer scanning of test items to create demonstrable 3D models. As compared to usual fluorescent microscopes, confocal microscopes are characterized by a higher contrast

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20

Plant, Randall L., and David H. Burns. "Confocal Scanning Slit Microscopy using a Linear Detector Array." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 172–73. http://dx.doi.org/10.1017/s0424820100158406.

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Confocal microscopy has been shown to produce improved lateral and axial image resolution compared with conventional microscopy. Image degradation due to scattering is reduced since only those portions of the specimen in the focal plane of the objective will contribute to image formation at the light detector. Previous work has shown that slit apertures can be used instead of pinholes to achieve the advantages of confocal microscopy with increased illumination and deceased scan line artifact. Slit scanning microscopes are also simpler in design than pinhole scanners but their aperture forms an

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21

Elaine C. Humphrey. "Teaching Microscopy by Workshop." Microscopy and Microanalysis 7, S2 (2001): 800–801. http://dx.doi.org/10.1017/s1431927600030075.

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The Biosciences Electron Microscopy Facility at UBC has at present two TEMs, one SEM, two confocals. several fluorescent light microscopes and an above average amount of digital imaging equipment used p by graduate students. The facility supports the Faculty of Medicine and the Faculty of Science and is run by a Director (myself) with one assistant. It is busy having an average of 600 individual users per year. The only way this number of users can be accommodated is by making them independent of staff. Training students had been previously on a one-to-one basis. This is very time consuming. F

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22

Stelzer, Ernst H. K., and Steffen Lindek. "3D Microscopy using Confocal Microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 54 (August 11, 1996): 270–71. http://dx.doi.org/10.1017/s0424820100163812.

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The importance of confocal fluorescence microscopy in modem biological research results from its optical sectioning capability, which allows the three-dimensional analysis of thick specimens. This property is due to the combination of a point-like light source and a point-like detector, which restrict the illumination and detection volumes, respectively. Only the volume that is illuminated and detected is relevant to the confocal observation volume. The smaller it is, the better is the resolution. The performance of a confocal microscope is thus primarily specified by the spatial extent of the

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23

Madrid-Wolff, Jorge, and Manu Forero-Shelton. "Protocol for the Design and Assembly of a Light Sheet Light Field Microscope." Methods and Protocols 2, no. 3 (2019): 56. http://dx.doi.org/10.3390/mps2030056.

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Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must col

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24

Monti, Manuela. "Basic confocal microscopy." European Journal of Histochemistry 56, no. 1 (2012): 3. http://dx.doi.org/10.4081/ejh.2012.br3.

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25

WILSON, T. "Confocal Light Microscopy." Annals of the New York Academy of Sciences 483, no. 1 Recent Advanc (1986): 416–27. http://dx.doi.org/10.1111/j.1749-6632.1986.tb34551.x.

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26

Petroll, W. Matthew, H. Dwight Cavanagh, and James V. Jester. "Clinical confocal microscopy." Current Opinion in Ophthalmology 9, no. 4 (1998): 59–65. http://dx.doi.org/10.1097/00055735-199808000-00011.

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27

Webb, Robert H. "Confocal optical microscopy." Reports on Progress in Physics 59, no. 3 (1996): 427–71. http://dx.doi.org/10.1088/0034-4885/59/3/003.

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28

Cox, Guy. "Biological confocal microscopy." Materials Today 5, no. 3 (2002): 34–41. http://dx.doi.org/10.1016/s1369-7021(02)05329-4.

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29

Müller, J., W. Ibach, K. Weishaupt, and O. Hollricher. "Confocal Raman Microscopy." Microscopy and Microanalysis 9, S02 (2003): 1084–85. http://dx.doi.org/10.1017/s143192760344542x.

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30

Summers, Robert. "Confocal Microscopy Listserver." Microscopy Today 2, no. 9 (1994): 8. http://dx.doi.org/10.1017/s1551929500067626.

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The Confocal microscopy listserver discussion group was put on the air over 5 years ago by Don Parsons (Albany), Steve Paddock (Wisconsin), P.C. Cheng (Buffalo) and me at the suggestion of Parsons that there should be an open discussion forum for this rapidly developing technology. Parsons is an expert in infomatics (among other things) and appreciated the potential of the “mail reflector” concept at an early stage in its development.Listserver groups were pretty uncommon then but we figured that since we were dealing with digital microscopists, most of those interested would have computers an

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31

Shahriari, Neda, Jane M. Grant-Kels, Harold Rabinovitz, Margaret Oliviero, and Alon Scope. "Reflectance confocal microscopy." Journal of the American Academy of Dermatology 84, no. 1 (2021): 1–14. http://dx.doi.org/10.1016/j.jaad.2020.05.153.

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32

Shahriari, Neda, Jane M. Grant-Kels, Harold Rabinovitz, Margaret Oliviero, and Alon Scope. "Reflectance confocal microscopy." Journal of the American Academy of Dermatology 84, no. 1 (2021): 17–31. http://dx.doi.org/10.1016/j.jaad.2020.05.154.

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33

Benedetti, P. A., V. Evangelista, D. Guidarini, and S. Vestri. "Confocal-line microscopy." Journal of Microscopy 165, no. 1 (1992): 119–29. http://dx.doi.org/10.1111/j.1365-2818.1992.tb04309.x.

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34

Evans, John A., and Norman S. Nishioka. "Endoscopic confocal microscopy." Current Opinion in Gastroenterology 21, no. 5 (2005): 578–84. http://dx.doi.org/10.1097/01.mog.0000174217.62214.10.

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35

Smith, Carolyn L. "Basic Confocal Microscopy." Current Protocols in Neuroscience 00, no. 1 (1997): 2.2.1–2.2.13. http://dx.doi.org/10.1002/0471142301.ns0202s00.

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36

Schatten, G., S. Paddock, P. Cooke, and J. Pawley. "Confocal microscopy at the integrated microscopy resource for biomedical research (IMR) of the university of wisconsin." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 92–93. http://dx.doi.org/10.1017/s0424820100102547.

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Confocal microscopy holds great promise for improved imaging of fluorescent or reflective biomedical specimens. The IMR is actively investigating the advantages and optimal usage of the Medical Research Council's Lasersharp laser - scanning confocal microscope and Tracor/Northern's Tandem Scanning Microscope, which benefits from the principles outlined by Petran et al. and Boyde.Quantitative evaluation of microscopic images has always been complicated by the effect of out-of-focus structures on the final image. These effects can be greatly reduced if the conventional light microscope is replac

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37

White, J. G., W. B. Amos, and M. Fordham. "An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy." Journal of Cell Biology 105, no. 1 (1987): 41–48. http://dx.doi.org/10.1083/jcb.105.1.41.

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Scanning confocal microscopes offer improved rejection of out-of-focus noise and greater resolution than conventional imaging. In such a microscope, the imaging and condenser lenses are identical and confocal. These two lenses are replaced by a single lens when epi-illumination is used, making confocal imaging particularly applicable to incident light microscopy. We describe the results we have obtained with a confocal system in which scanning is performed by moving the light beam, rather than the stage. This system is considerably faster than the scanned stage microscope and is easy to use. W

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38

McMillan, William. "Laser Scanning Confocal Microscopy for Materials Science." Microscopy Today 6, no. 5 (1998): 20–23. http://dx.doi.org/10.1017/s1551929500067791.

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Confocal microscopy has gained great popularity in biology and medical research because of the ability to image three-dimensional objects at greater resolution than conventional optical microscopes. In a typical Laser Scanning Confocal Microscope (LSCM), the specimen stage is stepped up or down to collect a series of two-dimensional images (or slices) at each focal plane. Conventional light microscopes create images with a depth of field, at high power, of 2 to 3 μm. The depth of field of confocal microscopes ranges from 0.5 to 1.5 μm, which allows information to be collected from a well defin

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39

Hell, Stefan W., Pekka E. Hanninen, Martin Schrader, Tony Wilson, and Erkki Soini. "Resolution beyond the diffraction limit: 4PI-confocal-, STED-, and GSD- fluorescence microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 53 (August 13, 1995): 56–57. http://dx.doi.org/10.1017/s0424820100136659.

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In far-field light microscopy resolution is determined by diffraction. In a far-field light microscope such as the confocal scanning light microscope, the resolution is governed by the extent of the squared intensity distribution in the focal region. Precise measurements of the confocal PSF have shown that the axial and lateral resolution of a confocal microscope (NA=1.4 oil, 1= 633 nm) is 520nm and 200nm (FWHM), respectively. At a wavelength of 375nm, this amounts to a resolution of 300 nm (axial) and 120 nm (lateral), obtainable with a standard confocal microscope of high aperture.A 3-7 fold

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40

W. Piston, David. "Two-Photon Excitation Imaging of Glucose Metabolism in Living Tissue." Microscopy and Microanalysis 3, S2 (1997): 305–6. http://dx.doi.org/10.1017/s1431927600008412.

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Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. It provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. This results in inherent submicron optical sectioning by excitation alone. In practice, TPEM is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing o

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41

Cherney, Daniel P. "Chemical Mapping of Rubbers and Polymers via Confocal Raman Spectroscopic Imaging." Rubber Chemistry and Technology 82, no. 4 (2009): 418–29. http://dx.doi.org/10.5254/1.3548255.

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Abstract Confocal Raman Spectroscopic Imaging has been demonstrated as a non-destructive technique to determine the spatial and chemical content of rubbers and polymers. Raman spectroscopy is a particularly useful tool for characterizing chemicals and mixtures because each chemically-distinct species has a unique Raman spectrum. The addition of confocal optics to the microscope greatly improves both the lateral and axial spatial resolution of the instrument. The lateral resolution of the instrument is less than one-third of a micron. The axial resolution, the resolution in the direction of inc

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42

Zhibin Wang, Zhibin Wang, Guohua Shi Guohua Shi, and Yudong Zhang Yudong Zhang. "Adaptive aberration correction in confocal scanning fluorescence microscopy." Chinese Optics Letters 12, s1 (2014): S11103–311105. http://dx.doi.org/10.3788/col201412.s11103.

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43

van Hook, Lee. "Entangled Microscopy." Microscopy Today 7, no. 3 (1999): 6–7. http://dx.doi.org/10.1017/s1551929500064038.

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The major factor in light microscopy limiting resolution in practice is not so much the wavelength of light and the resolving power of microscope optics, but the scattering of iight by the specimen. This extraneous scattered light interferes with the light used to image the specimen, effectively reducing the contrast of the imaging light and causing other annoying problems, There have been several inventions dedicated to solving this problem: various forms of interference-based microscopies, confocal microscopy, and most recently, multi-photon confocal microscopy.

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44

Keith, Charles H., and Mark A. Farmer. "Visualization of the Microtubules of Glutaraldehyde-Fixed Cells by Reflection-Enhanced Backscatter Confocal Microscopy." Microscopy and Microanalysis 12, no. 2 (2005): 113–23. http://dx.doi.org/10.1017/s1431927606060016.

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Performing reflection-mode (backscatter-mode) confocal microscopy on cells growing on reflective substrates gives images that have improved contrast and are more easily interpreted than standard reflection-mode confocal micrographs (Keith et al., 1998). However, a number of factors degrade the quality of images taken with the highest-resolution microscope objectives in this technique. We here describe modifications to reflection-enhanced backscatter confocal microscopy that (partially) overcome these factors. With these modifications of the technique, it is possible to visualize structures the

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45

Lemon, Gordon D., and Usher Posluszny. "A new approach to the study of apical meristem development using laser scanning confocal microscopy." Canadian Journal of Botany 76, no. 5 (1998): 899–904. http://dx.doi.org/10.1139/b98-043.

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Epi-illumination light microscopy and scanning electron microscopy have been standard techniques for developmental studies of shoot apices. Recently, laser scanning confocal microscopy has gained popularity as a tool for biological imaging. We have adapted laser scanning confocal microscopy to study development in whole shoot apices. It was tested on angiosperm and fern apices using three fluorescent dyes; acriflavine, safranin O, and acid fuchsin, and compared with epi-illumination light microscopy and scanning electron microscopy. In all cases, acid fuchsin proved to be the best fluorochrome

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46

Youngblom, Janey H., James J. Youngblom, and Jerry Wilkinson. "TelePresence Confocal Laser Scanning Microscopy." Microscopy and Microanalysis 7, no. 3 (2001): 241–48. http://dx.doi.org/10.1007/s100050010073.

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AbstractThe advent of the Internet has allowed the development of remote access capabilities to a growing variety and number of microscopy systems. To date, the confocal microscope has not been included among these systems. At the California State University (CSU) Confocal Microscopy Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system. Our Leica TCS NT confocal system is accessible to any authorized user via the Internet by using a free software program called VNC

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47

Juškaitis, R., N. P. Rea, and T. Wilson. "Semiconductor laser confocal microscopy." Applied Optics 33, no. 4 (1994): 578. http://dx.doi.org/10.1364/ao.33.000578.

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48

Linna, Tuuli, Helena Mikkil??, Anni Karma, Ilkka Sepp??l??, W. Matthew Petroll, and Timo Tervo. "In Vivo Confocal Microscopy." Cornea 15, no. 6 (1996): 639. http://dx.doi.org/10.1097/00003226-199611000-00018.

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Lisch, Walter. "In Vivo Confocal Microscopy." Cornea 15, no. 6 (1996): 640. http://dx.doi.org/10.1097/00003226-199611000-00019.

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&NA;. "CONFOCAL MICROSCOPY OF ACANTHAMOEBAKERATITIS." Cornea 20, no. 7 (2001): 778. http://dx.doi.org/10.1097/00003226-200110000-00030.

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