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1

Förster, Matthias, Marie-Alexandrine Bolzinger, Gilles Montagnac, and Stéphanie Briançon. "Confocal Raman microspectroscopy of the skin." European Journal of Dermatology 21, no. 6 (2011): 851–63. http://dx.doi.org/10.1684/ejd.2011.1494.

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2

Abramczyk, Halina, Beata Brozek-Pluska, Jakub Surmacki, Jacek Musial, and Radzislaw Kordek. "Oncologic photodynamic diagnosis and therapy: confocal Raman/fluorescence imaging of metal phthalocyanines in human breast cancer tissue in vitro." Analyst 139, no. 21 (2014): 5547–59. http://dx.doi.org/10.1039/c4an00966e.

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Raman microspectroscopy and confocal Raman imaging combined with confocal fluorescence were used to study the distribution and aggregation of aluminum tetrasulfonated phthalocyanine (AlPcS<sub>4</sub>) in breast tissues.
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3

Baldwin, K. J., and D. N. Batchelder. "Confocal Raman Microspectroscopy through a Planar Interface." Applied Spectroscopy 55, no. 5 (2001): 517–24. http://dx.doi.org/10.1366/0003702011952190.

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4

Edengeiser, Eugen, Konrad Meister, Erik Bründermann, Steffen Büning, Simon Ebbinghaus, and Martina Havenith. "Non-invasive chemical assessment of living human spermatozoa." RSC Advances 5, no. 14 (2015): 10424–29. http://dx.doi.org/10.1039/c4ra12158a.

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5

Braziel, S., K. Sullivan, and S. Lee. "Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer." Analyst 143, no. 3 (2018): 747–55. http://dx.doi.org/10.1039/c7an01349c.

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6

Lin, Shan-Yang, Ko-Hua Chen, Wen-Ting Cheng, Chi-Tien Ho та Shun-Li Wang. "Preliminary Identification of β-Carotene in the Vitreous Asteroid Bodies by Micro-Raman Spectroscopy and HPLC Analysis". Microscopy and Microanalysis 13, № 2 (2007): 128–32. http://dx.doi.org/10.1017/s143192760707002x.

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β-carotene was first identified from the vitreous asteroid bodies (ABs) excised from one patient with asteroid hyalosis (AH) by confocal Raman microspectroscopy and was also verified by high performance liquid chromatography (HPLC). Two patients had been diagnosed with AH and intervened by surgical vitrectomy due to blurred vision. The morphology and components of both AB specimens were observed by optical microscopy and determined by using confocal Raman microspectroscopy and HPLC analysis, respectively. Surprisingly, two unique peaks at 1528 and 1157 cm−1were found in the Raman spectrum for
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7

Tfaili, Sana, Cyril Gobinet, Gwendal Josse, Jean-François Angiboust, Michel Manfait, and Olivier Piot. "Confocal Raman microspectroscopy for skin characterization: a comparative study between human skin and pig skin." Analyst 137, no. 16 (2012): 3673–82. http://dx.doi.org/10.1039/c2an16292j.

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8

Yabumoto, Sohshi, and Hiro-o. Hamaguchi. "Tilted Two-Dimensional Array Multifocus Confocal Raman Microspectroscopy." Analytical Chemistry 89, no. 14 (2017): 7291–96. http://dx.doi.org/10.1021/acs.analchem.7b00614.

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9

Gallardo, A., R. Navarro, H. Reinecke, and S. Spells. "Correction of diffraction effects in confocal Raman microspectroscopy." Optics Express 14, no. 19 (2006): 8706. http://dx.doi.org/10.1364/oe.14.008706.

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10

Kador, Lothar, Tobias Schittkowski, Markus Bauer, and Yuwei Fan. "Three-dimensional materials analysis by confocal Raman microspectroscopy." Applied Optics 40, no. 28 (2001): 4965. http://dx.doi.org/10.1364/ao.40.004965.

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11

Sweetenham, Claire S., and Ioan Notingher. "Raman spectroscopy methods for detecting and imaging supported lipid bilayers." Spectroscopy 24, no. 1-2 (2010): 113–17. http://dx.doi.org/10.1155/2010/273028.

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We have developed a Raman microspectroscopy system optimised for studying supported lipid bilayers (SLB). This system combines the benefits of Raman spectroscopy with the high spatial resolution of confocal microscopy. Furthermore, the additional incorporation of an atomic force microscope (AFM) makes it possible to directly correlate chemical information with spatial features of samples at the nanoscale. We focus on the limits of this system for detecting a single SLB and imaging its microdomains, and employ surface-enhanced Raman spectroscopy (SERS) to improve the sensitivity achieved with R
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12

Patil, Chetan A., Christopher L. Arrasmith, Mark A. Mackanos, David L. Dickensheets, and Anita Mahadevan-Jansen. "A handheld laser scanning confocal reflectance imaging–confocal Raman microspectroscopy system." Biomedical Optics Express 3, no. 3 (2012): 488. http://dx.doi.org/10.1364/boe.3.000488.

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13

Blücher, Christina, Carolin Zilberfain, Tom Venus, et al. "Single cell study of adipose tissue mediated lipid droplet formation and biochemical alterations in breast cancer cells." Analyst 144, no. 18 (2019): 5558–70. http://dx.doi.org/10.1039/c9an00816k.

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14

Williams, K. P. J., G. D. Pitt, D. N. Batchelder, and B. J. Kip. "Confocal Raman Microspectroscopy Using a Stigmatic Spectrograph and CCD Detector." Applied Spectroscopy 48, no. 2 (1994): 232–35. http://dx.doi.org/10.1366/0003702944028407.

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Confocal Raman microspectroscopy has previously used pinholes placed at the back focal plane of the microscope to provide depth resolution along the optical axis. The process of optimizing the pinhole alignment can often be difficult and time-consuming. We demonstrate a different approach to setting up a confocal Raman microscope using a stigmatic spectrograph and a CCD detector. This arrangement is easy to use and provides a depth resolution of ∼2 μm.
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15

Kochan, K., K. M. Marzec, E. Maslak, S. Chlopicki, and M. Baranska. "Raman spectroscopic studies of vitamin A content in the liver: a biomarker of healthy liver." Analyst 140, no. 7 (2015): 2074–79. http://dx.doi.org/10.1039/c4an01878h.

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16

Tabaksblat, Ronald, Robert J. Meier, and Bert J. Kip. "Confocal Raman Microspectroscopy: Theory and Application to Thin Polymer Samples." Applied Spectroscopy 46, no. 1 (1992): 60–68. http://dx.doi.org/10.1366/0003702924444434.

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Raman microspectroscopy can be used effectively to study very small samples or to study small areas within a transparent sample. With the application of the technique of confocal microscopy to a Raman microscope, the depth resolution of the instrument can be enhanced considerably. Confocal microscopy uses a pinhole, placed in the back image plane of the microscope objective, to block light from outside the focal plane. In this way the signal from the small volume element one wants to study can be better separated from the signals arising from the surrounding material. In this paper we show tha
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17

Tfaili, Sana, Cyril Gobinet, Gwendal Josse, Jean-François Angiboust, Michel Manfait, and Olivier Piot. "Correction: Confocal Raman microspectroscopy for skin characterization: a comparative study between human skin and pig skin." Analyst 145, no. 13 (2020): 4699–700. http://dx.doi.org/10.1039/d0an90060e.

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Correction for ‘Confocal Raman microspectroscopy for skin characterization: a comparative study between human skin and pig skin’ by Sana Tfaili et al., Analyst, 2012, 137, 3673–3682, DOI: 10.1039/C2AN16292J.
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18

Kong, Lingbo, Pengfei Zhang, Peter Setlow, and Yong-qing Li. "Multifocus confocal Raman microspectroscopy for rapid single-particle analysis." Journal of Biomedical Optics 16, no. 12 (2011): 120503. http://dx.doi.org/10.1117/1.3662456.

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19

Maquelin, K., L. P. Choo-Smith, H. P. Endtz, H. A. Bruining, and G. J. Puppels. "Rapid Identification of Candida Species by Confocal Raman Microspectroscopy." Journal of Clinical Microbiology 40, no. 2 (2002): 594–600. http://dx.doi.org/10.1128/jcm.40.2.594-600.2002.

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20

Tomba, J. Pablo, José M. Carella, and José M. Pastor. "Interphase Evolution in Polymer Films by Confocal Raman Microspectroscopy." Applied Spectroscopy 60, no. 2 (2006): 115–21. http://dx.doi.org/10.1366/000370206776023377.

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21

Puppels, G. J., C. Otto, and J. Greve. "Confocal Raman microspectroscopy in biology: Applications and future developments." TrAC Trends in Analytical Chemistry 10, no. 8 (1991): 249–53. http://dx.doi.org/10.1016/0165-9936(91)85131-a.

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22

Bednárová, Lucie, Jan Palacký, Václava Bauerová, Olga Hrušková-Heidingsfeldová, Iva Pichová, and Peter Mojzeš. "Raman Microspectroscopy of the Yeast Vacuoles." Spectroscopy: An International Journal 27 (2012): 503–7. http://dx.doi.org/10.1155/2012/746597.

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In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was investigated and critically assessed. Simple, economical, and practical protocols of the yeast immobilization suitable for less laborious, high-throughput, and spatially resolved Raman measurements were tested for their possible impacts on physiological states and viability of the cells. We have demonstrated that, acquiring Raman spectra from statistically sound sets of immobilized cells and employing advanced multivariate methods for spectral an
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23

Wang, Shuang, Jianhua Zhao, Harvey Lui, Qingli He, and Haishan Zeng. "A modular Raman microspectroscopy system for biological tissue analysis." Spectroscopy 24, no. 6 (2010): 577–83. http://dx.doi.org/10.1155/2010/592315.

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Raman spectroscopy has been used as a sensitive tool for studying biological tissue and evaluating disease. In many applications, microscopic level resolution spectral analysis is desirable. And this has been performed mostly by expensive commercial confocal micro-Raman systems. In this research, we present a simple method for building an economical and modular Raman microspectroscopy system that combines a microscope with a Raman spectrometer using an optical fiber bundle. The bundle with a circular collection end is positioned at an image plane of the microscope to collect Raman signals from
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24

Moissette, A., M. Hureau, M. Moreau, and J. P. Cornard. "Pore selectivity and electron transfers in HZSM-5 single crystals: a Raman microspectroscopy mapping and confocal fluorescence imaging combined study." Physical Chemistry Chemical Physics 22, no. 22 (2020): 12745–56. http://dx.doi.org/10.1039/d0cp02018d.

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Electron transfers at the single particle level in HZSM-5 zeolite are followed by combining Raman microspectroscopy mapping and confocal fluorescence imaging. The effects of pore accessibility and guest diffusion on reactivity are investigated.
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25

Hsieh, Tzu-Feng, Ken-Jen Yu, and Shan-Yang Lin. "Possible Application of Raman Microspectroscopy to Verify the Interstitial Cystitis Diagnosis after Potassium Sensitivity Test: Phenylalanine or Tryptophan as a Biomarker." Disease Markers 23, no. 3 (2007): 147–52. http://dx.doi.org/10.1155/2007/705630.

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There is lack of a worldwide standard technique for clinical diagnosis of interstitial cystitis (IC). Raman spectroscopy with higher specificity and sensitivity has been extensively used to act as a non-destructive analytical technique without special sample preparation. In this preliminary study, possible use of Raman microspectroscopy as an IC diagnostic tool was attempted. Twenty-two participants were screened by clinical features, history, urodynamic evaluations and potassium sensitivity test (PST). The freeze-dried water samples voided from all the participants after PST were directly det
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26

Wang, Fengping, Xingtai Zhou, Jigang Zhou, Tsun-Kong Sham, and Zhifeng Ding. "Observation of Single Tin Dioxide Nanoribbons by Confocal Raman Microspectroscopy." Journal of Physical Chemistry C 111, no. 51 (2007): 18839–43. http://dx.doi.org/10.1021/jp709701s.

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27

Puppels, G. J., F. F. M. de Mul, C. Otto, et al. "Studying single living cells and chromosomes by confocal Raman microspectroscopy." Nature 347, no. 6290 (1990): 301–3. http://dx.doi.org/10.1038/347301a0.

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28

Sacristán, Javier, Carmen Mijangos, Helmut Reinecke, Stephen Spells, and Jack Yarwood. "Depth profiling of modified PVC surfaces using confocal Raman microspectroscopy." Macromolecular Rapid Communications 21, no. 13 (2000): 894–96. http://dx.doi.org/10.1002/1521-3927(20000801)21:13<894::aid-marc894>3.0.co;2-1.

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29

Tfaili, Sana, Gwendal Josse, Jean-François Angiboust, Michel Manfait, and Olivier Piot. "Monitoring caffeine and resveratrol cutaneous permeation by confocal Raman microspectroscopy." Journal of Biophotonics 7, no. 9 (2013): 676–81. http://dx.doi.org/10.1002/jbio.201300011.

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30

Otto, C., N. M. Sijtsema, and J. Greve. "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes." European Biophysics Journal 27, no. 6 (1998): 582–89. http://dx.doi.org/10.1007/s002490050169.

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31

Vyörykkä, J., J. Paaso, M. Tenhunen, et al. "Analysis of Depth Profiling Data Obtained by Confocal Raman Microspectroscopy." Applied Spectroscopy 57, no. 9 (2003): 1123–28. http://dx.doi.org/10.1366/00037020360695982.

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32

Yu, Chenxu, Erin Gestl, Kristin Eckert, David Allara, and Joseph Irudayaraj. "Characterization of human breast epithelial cells by confocal Raman microspectroscopy." Cancer Detection and Prevention 30, no. 6 (2006): 515–22. http://dx.doi.org/10.1016/j.cdp.2006.10.007.

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33

Kunstar, Aliz, Anne M. Leferink, Paul I. Okagbare, et al. "Label-free Raman monitoring of extracellular matrix formation in three-dimensional polymeric scaffolds." Journal of The Royal Society Interface 10, no. 86 (2013): 20130464. http://dx.doi.org/10.1098/rsif.2013.0464.

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Monitoring extracellular matrix (ECM) components is one of the key methods used to determine tissue quality in three-dimensional scaffolds for regenerative medicine and clinical purposes. Raman spectroscopy can be used for non-invasive sensing of cellular and ECM biochemistry. We have investigated the use of conventional (confocal and semiconfocal) Raman microspectroscopy and fibre-optic Raman spectroscopy for in vitro monitoring of ECM formation in three-dimensional poly(ethylene oxide terephthalate)–poly(butylene terephthalate) (PEOT/PBT) scaffolds. Chondrocyte-seeded PEOT/PBT scaffolds were
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34

Tomba, J. Pablo, Luis M. Arzondo, and José M. Pastor. "Depth Profiling by Confocal Raman Microspectroscopy: Semi-Empirical Modeling of the Raman Response." Applied Spectroscopy 61, no. 2 (2007): 177–85. http://dx.doi.org/10.1366/000370207779947477.

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35

Richters, Renée J. H., Denise Falcone, Natallia E. Uzunbajakava, et al. "Sensitive Skin: Assessment of the Skin Barrier Using Confocal Raman Microspectroscopy." Skin Pharmacology and Physiology 30, no. 1 (2017): 1–12. http://dx.doi.org/10.1159/000452152.

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36

Wang, Yong, and Paulette Spencer. "Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy." Journal of Biomedical Materials Research 59, no. 1 (2001): 46–55. http://dx.doi.org/10.1002/jbm.1215.

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37

Stamatas, G., E. Boireau-Adamezyk, and T. Oddos. "690 In vivo assessment of cleanser mildness by confocal raman microspectroscopy." Journal of Investigative Dermatology 138, no. 5 (2018): S117. http://dx.doi.org/10.1016/j.jid.2018.03.699.

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38

Wu, J., and T. G. Polefka. "Confocal Raman microspectroscopy of stratum corneum: a pre-clinical validation study." International Journal of Cosmetic Science 30, no. 1 (2008): 47–56. http://dx.doi.org/10.1111/j.1468-2494.2008.00428.x.

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39

Ye, Yuhuang, Yang Chen, Yongzeng Li, et al. "Characterization and discrimination of nasopharyngeal carcinoma and nasopharyngeal normal cell lines using confocal Raman microspectroscopy." Spectroscopy 25, no. 5 (2011): 217–24. http://dx.doi.org/10.1155/2011/405457.

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Raman microspectroscopy can provide molecular-level information about the biochemical composition and structure of cells and tissues with excellent spatial resolution. In this study, Raman spectroscopy of individual cells from nasopharyngeal carcinoma cell lines C666-1, CNE2 and nasopharyngeal normal cell line NP69 are investigated for their differences. The spectral intensity ratio at 1449 and 1657 cm−1with a decision line of I1449/I1657=1.10 can very easily separate the tumor and normal cell lines into two groups. Principal component analysis (PCA) and linear discriminant analysis (LDA) are
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40

Feofanov, Alexei V., Alexei I. Grichine, Larissa A. Shitova, et al. "Confocal Raman Microspectroscopy and Imaging Study of Theraphthal in Living Cancer Cells." Biophysical Journal 78, no. 1 (2000): 499–512. http://dx.doi.org/10.1016/s0006-3495(00)76612-4.

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41

Sacristán, Javier, Carmen Mijangos, Helmut Reinecke, Stephen Spells, and Jack Yarwood. "Selective Surface Modification of PVC Films As Revealed by Confocal Raman Microspectroscopy." Macromolecules 33, no. 16 (2000): 6134–39. http://dx.doi.org/10.1021/ma000272m.

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42

Okuno, Masanari, and Hiro-o. Hamaguchi. "Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells." Optics Letters 35, no. 24 (2010): 4096. http://dx.doi.org/10.1364/ol.35.004096.

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43

Tomba, J. Pablo, María de la Paz Miguel, and Claudio J. Perez. "Correction of optical distortions in dry depth profiling with confocal Raman microspectroscopy." Journal of Raman Spectroscopy 42, no. 6 (2011): 1330–34. http://dx.doi.org/10.1002/jrs.2843.

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44

Terentis, Andrew C., Sara A. Fox, Samantha J. Friedman, and Emily S. Spencer. "Confocal Raman microspectroscopy discriminates live human metastatic melanoma and skin fibroblast cells." Journal of Raman Spectroscopy 44, no. 9 (2013): 1205–16. http://dx.doi.org/10.1002/jrs.4363.

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45

Gong, Aiping, Weimin Gu, Zhenyu Zhao, and Yongni Shao. "Identification of heavy metal by testing microalgae using confocal Raman microspectroscopy technology." Applied Optics 58, no. 31 (2019): 8396. http://dx.doi.org/10.1364/ao.58.008396.

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46

Djaker, Nadia, Claudine Wulfman, Michaël Sadoun, and Marc Lamy de la Chapelle. "Zirconia dental implants degradation by confocal Raman microspectroscopy: analytical simulation and experiments." Biomedical Optics Express 4, no. 5 (2013): 725. http://dx.doi.org/10.1364/boe.4.000725.

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47

Hajatdoost, Sohail, and Jack Yarwood. "Depth Profiling of Poly(Methyl Methacrylate), Poly(Vinyl Alcohol) Laminates by Confocal Raman Microspectroscopy." Applied Spectroscopy 50, no. 5 (1996): 558–64. http://dx.doi.org/10.1366/0003702963905961.

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We report a molecular depth profiling study of a PMMA/PVOH laminate on quartz using confocal Raman microspectroscopy. It is demonstrated that this technique can be successfully employed to study the hydrogen-bonding interaction between the ester and alcohol groups near the interfacial region. The carbonyl, v(C=O), band of PMMA shows significant broadening in the interfacial region. Various PMMA/PVOH laminates with different PMMA molecular weights have been studied, and it is demonstrated that the PMMA layers with lower molecular weight show a greater degree of interpenetration for a given anne
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48

Pelletier, M. J., J. Slater, K. L. Davis, W. K. Kowalchyk, and I. R. Lewis. "Robust Fiber-Optic Coupled Confocal Raman Microscopy for Research and Quality Control Applications." Microscopy and Microanalysis 3, S2 (1997): 823–24. http://dx.doi.org/10.1017/s1431927600011004.

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In this paper we report the continuing development of a novel fiber-optic coupled confocal Raman microscope which can offer spectral resolutions up to 0.6 cm−1 per pixel, spatial resolutions of 1 micron or less with axial resolutions of 2-3 microns. The system is based around a compact base unit which comprises a compact solid-state laser, a proprietary f/1.8 imaging spectrograph, and a TE-cooled CCD detector operating at −70°C.In Figure 1 a schematic of the fiber coupled microscope is shown. The microscope includes a integrated holographic filter module to prevent silica Raman generated in th
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49

Sun, Yingying, Masahiro Yanagisawa, Masahiro Kunimoto, Masatoshi Nakamura, and Takayuki Homma. "Depth profiling of APTES self-assembled monolayers using surface-enhanced confocal Raman microspectroscopy." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 184 (September 2017): 1–6. http://dx.doi.org/10.1016/j.saa.2017.04.036.

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50

Tomba, J. Pablo, and José M. Pastor. "Confocal Raman microspectroscopy with dry objectives: A depth profiling study on polymer films." Vibrational Spectroscopy 44, no. 1 (2007): 62–68. http://dx.doi.org/10.1016/j.vibspec.2006.08.001.

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