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Journal articles on the topic 'Confocal scanning laser microscopy'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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1

J. H., Youngblom, Wilkinson J., and Youngblom J.J. "Telepresence Confocal Microscopy." Microscopy and Microanalysis 6, S2 (August 2000): 1164–65. http://dx.doi.org/10.1017/s1431927600038319.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments. While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal laser scanning microscope.At California State University-Stanislaus, home of the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system. Our Leica TCS NT confocal system, with an interchangeable upright (DMRXE) and inverted microscope (DMIRBE) set up,

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Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Telepresence Confocal Microscopy." Microscopy Today 8, no. 10 (December 2000): 20–21. http://dx.doi.org/10.1017/s1551929500054146.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments, While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal laser scanning microscope.

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3

Paddock, Stephen W. "Confocal Laser Scanning Microscopy." BioTechniques 27, no. 5 (November 1999): 992–1004. http://dx.doi.org/10.2144/99275ov01.

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4

HASEGAWA, Hirokazu. "Laser Scanning Confocal Microscopy." Kobunshi 55, no. 12 (2006): 961–65. http://dx.doi.org/10.1295/kobunshi.55.961.

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5

Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Confocal Laser Scanning Microscopy By Remote Access." Microscopy Today 7, no. 7 (September 1999): 32–33. http://dx.doi.org/10.1017/s1551929500064798.

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In recent years there have been a growing number of facilities interested in developing remote access capabilities to a variety of microscopy systems. While certain types of microscopes, such as electron microscopes and scanning probe microscopes have been well established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal microscope.At California State University, home to the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system.

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Yimei Huang, Yimei Huang, Hongqin Yang Hongqin Yang, Xiuqiu Shen Xiuqiu Shen, Yuhua Wang Yuhua Wang, Liqin Zheng Liqin Zheng, Hui Li Hui Li, and Shusen Xie Shusen Xie. "Visualizing NO in live cells by confocal laser scanning microscopy." Chinese Optics Letters 10, s1 (2012): S11701–311703. http://dx.doi.org/10.3788/col201210.s11701.

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7

Youngblom, Janey H., James J. Youngblom, and Jerry Wilkinson. "TelePresence Confocal Laser Scanning Microscopy." Microscopy and Microanalysis 7, no. 3 (May 2001): 241–48. http://dx.doi.org/10.1007/s100050010073.

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AbstractThe advent of the Internet has allowed the development of remote access capabilities to a growing variety and number of microscopy systems. To date, the confocal microscope has not been included among these systems. At the California State University (CSU) Confocal Microscopy Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system. Our Leica TCS NT confocal system is accessible to any authorized user via the Internet by using a free software program called VNC (Virtual Network Computing). Once connectivity is established, remote users are able to control virtually all the functions to conduct real-time image analysis and quantitative assessments of their specimen. They can also move the motorized stage to view different regions of their specimen by using a software program associated with the stage. At the end of the session, all files generated during the session can be downloaded to the user’s computer from a link on the CSU confocal website. A number of safeguard features have been developed to ensure security and privacy of data acquired during a remote session.

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8

Raarup, Merete Krog, and Jens Randel Nyengaard. "QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY." Image Analysis & Stereology 25, no. 3 (May 3, 2011): 111. http://dx.doi.org/10.5566/ias.v25.p111-120.

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This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation. The use of CLSM for improved stereological length estimation in thick (up to 0.5 mm) tissue is proposed. The techniques of FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), FCS (Fluorescence Correlation Spectroscopy) and FRAP (Fluorescence Recovery After Photobleaching) are introduced and their applicability for quantitative imaging of biomolecular (co-)localization and trafficking in live cells described. The advantage of two-photon versus one-photon excitation in relation to these techniques is discussed.

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9

Youngblom, Janey H., James J. Youngblom, and Jerry Wilkinson. "TelePresence Confocal Laser Scanning Microscopy." Microscopy and Microanalysis 7, no. 03 (May 2001): 241–48. http://dx.doi.org/10.1017/s1431927601010248.

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10

Bayguinov, Peter O., Dennis M. Oakley, Chien-Cheng Shih, Daniel J. Geanon, Matthew S. Joens, and James A. J. Fitzpatrick. "Modern Laser Scanning Confocal Microscopy." Current Protocols in Cytometry 85, no. 1 (June 20, 2018): e39. http://dx.doi.org/10.1002/cpcy.39.

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11

Fabich, Markus. "Advancing Confocal Laser Scanning Microscopy." Optik & Photonik 4, no. 2 (June 2009): 40–43. http://dx.doi.org/10.1002/opph.201190025.

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12

McMillan, William. "Laser Scanning Confocal Microscopy for Materials Science." Microscopy Today 6, no. 5 (July 1998): 20–23. http://dx.doi.org/10.1017/s1551929500067791.

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Confocal microscopy has gained great popularity in biology and medical research because of the ability to image three-dimensional objects at greater resolution than conventional optical microscopes. In a typical Laser Scanning Confocal Microscope (LSCM), the specimen stage is stepped up or down to collect a series of two-dimensional images (or slices) at each focal plane. Conventional light microscopes create images with a depth of field, at high power, of 2 to 3 μm. The depth of field of confocal microscopes ranges from 0.5 to 1.5 μm, which allows information to be collected from a well defined optical section rather than from most of the specimen. Therefore, due to this “thin” focal plane, out of focus light is virtually eliminated which results in an increase in contrast, clarity and detection.

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13

Pironon, Jacques, Martin Canals, Jean Dubessy, Frédéric Walgenwitz, and Cοrinne Laplace-Builhe. "Volumetric reconstruction of individual oil inclusions by confocal scanning laser microscopy." European Journal of Mineralogy 10, no. 6 (December 1, 1998): 1143–50. http://dx.doi.org/10.1127/ejm/10/6/1143.

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14

Cheng, P. C., S. J. Pan, A. Shih, W. S. Liou, M. S. Park, T. Watson, J. Bhawalkar, and P. Prasard. "Two-Photon Laser Scanning Confocal Microscopy." Microscopy and Microanalysis 3, S2 (August 1997): 847–48. http://dx.doi.org/10.1017/s1431927600011120.

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Two-photon fluorescence microscopy has become an important research tool in both biological and material sciences. The technique uses long wavelength, typically in the near IR, as the excitation light to obtain shorter wavelength fluorescence (e.g. visible light). Because of the low linear absorption coefficient of most biological and polymeric specimens, this technique allows deeper penetration of the excitation beam, achieving optical sectioning to a depth of 250μm or more into the specimen. As a result of the quadratic dependency of the two-photon induced fluorescence to the excitation intensity, the fluorescent emission and photobleaching are limited to the vicinity of focal spot. This capability of addressing a specimen’s 3D space allows exciting possibilities in biological researches, such as 3D photobleaching recovery experiment.Two-photon confocal fluorescence microscopy is ideal for the study of thick biological and material specimen in 3D. For example, Figure 1 shows a three-dimensional isosurface rendered image of a vascular bundle from a maize stem.

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15

GROSS, Janet, and Gavin BECKER. "Confocal laser scanning microscopy in nephrology." Nephrology 1, no. 3 (June 1995): 175–79. http://dx.doi.org/10.1111/j.1440-1797.1995.tb00025.x.

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16

Bacon, Jonathan P., Cytano Gonzalez, and Christopher J. Hutchinson. "Applications of confocal laser scanning microscopy." Trends in Cell Biology 1, no. 6 (December 1991): 172–75. http://dx.doi.org/10.1016/0962-8924(91)90019-6.

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17

Lemon, Gordon D., and Usher Posluszny. "A new approach to the study of apical meristem development using laser scanning confocal microscopy." Canadian Journal of Botany 76, no. 5 (May 1, 1998): 899–904. http://dx.doi.org/10.1139/b98-043.

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Epi-illumination light microscopy and scanning electron microscopy have been standard techniques for developmental studies of shoot apices. Recently, laser scanning confocal microscopy has gained popularity as a tool for biological imaging. We have adapted laser scanning confocal microscopy to study development in whole shoot apices. It was tested on angiosperm and fern apices using three fluorescent dyes; acriflavine, safranin O, and acid fuchsin, and compared with epi-illumination light microscopy and scanning electron microscopy. In all cases, acid fuchsin proved to be the best fluorochrome for examining shoot apices; having a high affinity for cell walls and nuclear material. The images produced with laser scanning confocal microscopy were sharper and clearer than images generated with epi-illumination light microscopy and scanning electron microscopy. Laser scanning confocal microscopy allows one to map patterns of cell division on the surface of an apical meristem, which is extremely difficult using other techniques such as scanning electron microscopy or epi-illumination light microscopy. Since the laser scanning light microscope records images digitally a method for digital plate production is described. Our methods can easily be applied to study the development of other plant structures on a cellular level such as root apical meristems, floral meristems, stomata, or trichomes, and reproductive organs in lower plants.Key words: confocal microscopy, apical meristem, development, fluorochrome, cytokinesis.

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18

Cooper, M. S. "Imaging cellular dynamics using scanning laser confocal microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 1 (August 1992): 12–13. http://dx.doi.org/10.1017/s0424820100120461.

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In recent years, the ability to image morphological dynamics and physiological changes in living cells and tissues has been greatly advanced by the advent of scanning laser confocal microscopy. Confocal microscopes employ optical systems in which both the condenser and objective lenses are focused onto a single volume element of the specimen. In practice, galvanometer-driven mirrors or acousto-optical deflectors are used to scan a laser beam over the specimen in a raster-like fashion through an epifluorescence microscope. The incident laser beam, as well as the collected fluorescent light, are passed through pinhole or slit apertures in image planes that are conjugate to the plane of the specimen. This method of illumination and detection prevents fluorescent light which is generated above and below the plane-of-focus from impinging on the imaging system's photodetector, thus rejecting much of the fluorescent light which normally blurs the image of a three-dimensional fluorescent specimen.

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19

Carlsson, K., P. E. Danielsson, A. Liljeborg, L. Majlöf, R. Lenz, and N. Åslund. "Three-dimensional microscopy using a confocal laser scanning microscope." Optics Letters 10, no. 2 (February 1, 1985): 53. http://dx.doi.org/10.1364/ol.10.000053.

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20

Wessendorf, Martin W., and T. Clark Brelje. "Multicolor Fluorescence Microscopy Using the Laser-Scanning Confocal Microscope." Neuroprotocols 2, no. 2 (April 1993): 121–40. http://dx.doi.org/10.1006/ncmn.1993.1017.

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21

Xie, Huimin, Satoshi Kishimoto, Bing Pan, Yanjie Li, Qinghua Wang, and Zhiqiang Guo. "OS1-2-3 Experimental Study on the Laser Scanning Confocal Microscopy Moire Method." Abstracts of ATEM : International Conference on Advanced Technology in Experimental Mechanics : Asian Conference on Experimental Mechanics 2007.6 (2007): _OS1–2–3–1—_OS1–2–3–4. http://dx.doi.org/10.1299/jsmeatem.2007.6._os1-2-3-1.

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22

Atkinson, Matthew R. "Polymer Characterization Using Confocal Scanning Laser Microscopy: A Review." Microscopy and Microanalysis 5, S2 (August 1999): 988–89. http://dx.doi.org/10.1017/s1431927600018262.

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Confocal microscopy was developed in 1957 by Minski, who was awarded a patent for this work in 1961. Since that time many advances have been in new designs and implementations. There are two basic classes of confocal microscope: the Nipkow-disk-based confocal microscope which allow real-time direct viewing of the sample, and the confocal scanning laser microscopes (CSLM). The CSLM will be focussed on in this presentation.The CSLM scans a focussed beam over, across or through the sample, collecting the reflected, scattered or emitted light. This light is directed towards an optical spatial filter, which passes light returning from the on-axis focus position, and rejects light that is returning from anywhere else. By detecting the light passing through the spatial filter synchronously with the moving beam and/or sample, an image that has only in-focus information is acquired. Typically a set of images is acquired as the sample is moved through focus: from this “image stack” both an extended-focus image and topographic information can be obtained.

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23

Fujii, H., D. J. Wood, J. M. Papadimitriou, and M. H. Zheng. "Application of Confocal Laser Scanning Microscopy in Bone." Journal of Musculoskeletal Research 02, no. 01 (March 1998): 65–71. http://dx.doi.org/10.1142/s0218957798000093.

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The optical sectioning method of confocal laser scanning microscopy provides higher resolution than standard light microscope techniques. The use of optical rather than physical sections for detailed histological analyses of bone obviates the need for either decalcification or complex plastic embedding processes which are required as a routine for the preparation of thin microtome sections. In this study we have used confocal laser scanning microscopy for the morphological analyses of fresh unembedding human cortical bone, bone allograft and bone cement interfaces. Our results have indicated that such an approach has provided a relatively easy and rapid means for the assessment of the histology of normal and pathological bone.

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24

ZHAO Wei-qian, 赵维谦, 任利利 REN Li-li, 盛. 忠. SHENG Zhong, 王. 允. WANG Yun, and 邱丽荣 QIU Li-rong. "Beam deflection scanning for laser confocal microscopy." Optics and Precision Engineering 24, no. 6 (2016): 1257–63. http://dx.doi.org/10.3788/ope.20162406.1257.

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25

Földes-Papp, Zeno, Ulrike Demel, and Gernot P. Tilz. "Laser scanning confocal fluorescence microscopy: an overview." International Immunopharmacology 3, no. 13-14 (December 2003): 1715–29. http://dx.doi.org/10.1016/s1567-5769(03)00140-1.

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26

Jones, C. W., D. Smolinski, A. Keogh, T. B. Kirk, and M. H. Zheng. "Confocal laser scanning microscopy in orthopaedic research." Progress in Histochemistry and Cytochemistry 40, no. 1 (May 2005): 1–71. http://dx.doi.org/10.1016/j.proghi.2005.02.001.

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27

Liu, Changgeng. "Spatial heterodyne scanning laser confocal holographic microscopy." Applied Optics 54, no. 34 (November 25, 2015): 10096. http://dx.doi.org/10.1364/ao.54.010096.

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28

KITAGAWA, Junichi. "Confocal Laser Scanning Microscopy for Biological Use." Journal of the Japan Society for Precision Engineering 72, no. 11 (2006): 1331–34. http://dx.doi.org/10.2493/jjspe.72.1331.

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29

HAMILTON, PETER W., and COLIN F. JOHNSTON. "DNA PLOIDY BY CONFOCAL LASER SCANNING MICROSCOPY." Journal of Pathology 181, no. 1 (January 1997): 1–2. http://dx.doi.org/10.1002/(sici)1096-9896(199701)181:1<1::aid-path718>3.0.co;2-i.

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30

Johnston, C. F., C. Shaw, D. W. Halton, and I. Fairweather. "Confocal scanning laser microscopy and helminth neuroanatomy." Parasitology Today 6, no. 9 (September 1990): 305–8. http://dx.doi.org/10.1016/0169-4758(90)90262-3.

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31

Ling, X., M. D. Pritzker, J. J. Byerley, and C. M. Burns. "Confocal scanning laser microscopy of polymer coatings." Journal of Applied Polymer Science 67, no. 1 (January 3, 1998): 149–58. http://dx.doi.org/10.1002/(sici)1097-4628(19980103)67:1<149::aid-app17>3.0.co;2-x.

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32

Schnitzler, Lena, Markus Finkeldey, Martin R. Hofmann, and Nils C. Gerhardt. "Contrast Enhancement for Topographic Imaging in Confocal Laser Scanning Microscopy." Applied Sciences 9, no. 15 (July 31, 2019): 3086. http://dx.doi.org/10.3390/app9153086.

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The influence of the axial pinhole position in a confocal microscope in terms of the contrast of the image is analyzed. The pinhole displacement method is introduced which allows to increase the contrast for topographic imaging. To demonstrate this approach, the simulated data of a confocal setup as well as experimental data is shown. The simulated data is verified experimentally by a custom stage scanning reflective microscopy setup using a semiconductor test target with low contrast structures of sizes between 200 nm and 500 nm. With the introduced technique, we are able to achieve a contrast enhancement of up to 80% without loosing diffraction limited resolution. We do not add additional components to the setup, thus our concept is applicable for all types of confocal microscopes. Furthermore, we show the application of the contrast enhancement in imaging integrated circuits.

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33

Matsumoto, Tomoko, Ryuichi Shiraki, Jin Matsumoto, Tsutomu Shiragami, and Masahide Yasuda. "Microscopic Spectrophotometry Using Confocal Laser Scanning Microscope." BUNSEKI KAGAKU 57, no. 10 (2008): 819–24. http://dx.doi.org/10.2116/bunsekikagaku.57.819.

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34

Kamanyi, Albert, Wilfred Ngwa, Timo Betz, Reinhold Wannemacher, and Wolfgang Grill. "Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy." Ultrasonics 44 (December 2006): e1295-e1300. http://dx.doi.org/10.1016/j.ultras.2006.05.030.

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35

Schatten, G., S. Paddock, P. Cooke, and J. Pawley. "Confocal microscopy at the integrated microscopy resource for biomedical research (IMR) of the university of wisconsin." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 92–93. http://dx.doi.org/10.1017/s0424820100102547.

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Confocal microscopy holds great promise for improved imaging of fluorescent or reflective biomedical specimens. The IMR is actively investigating the advantages and optimal usage of the Medical Research Council's Lasersharp laser - scanning confocal microscope and Tracor/Northern's Tandem Scanning Microscope, which benefits from the principles outlined by Petran et al. and Boyde.Quantitative evaluation of microscopic images has always been complicated by the effect of out-of-focus structures on the final image. These effects can be greatly reduced if the conventional light microscope is replaced by a scanning-confocal light microscope. In such an instrument two conditions are met: 1) only a single point of the sample is illuminated at any time and 2) this point on the sample is then imaged onto the pinhole at the entrance to the photodetector. Because little light from out-of-focus planes will pass through the pinhole, only in-focus data is recorded.

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OKA, Kotaro, and Kazuo TANISHITA. "Biological Transport Analysis by Confocal Laser Scanning Microscopy." JOURNAL OF JAPAN SOCIETY FOR LASER SURGERY AND MEDICINE 16, no. 4 (1995): 33–39. http://dx.doi.org/10.2530/jslsm1980.16.4_33.

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37

Balzano, Angela, Klemen Novak, Miha Humar, and Katarina Čufar. "Application of confocal laser scanning microscopy in dendrochronology." Les/Wood 68, no. 2 (December 30, 2019): 5–17. http://dx.doi.org/10.26614/les-wood.2019.v68n02a01.

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We used the Confocal Laser Scanning Microscope (CLSM) Olympus LEXT OLS5000 for non-destructive observation and image analysis of wood anatomy traits in growth layers of tree species from different climatic zones. In European beech (Fagus sylvatica), where tree rings can generally be recognised, we discuss the changes in tree-ring structure due to adverse effects (insect attacks). Growth layers in Mediterranean Aleppo pine (Pinus halepensis) from south-eastern Spain are not always annual and contain numerous intra-annual density fluctuations (IADFs). Ocote pine (Pinus oocarpa) growing at high elevation in Honduras showed growth layers with clear growth ring boundaries and IADFs. In both pines, CLSM allowed us to recognise and measure tracheid parameters to define density fluctuations. In tropical true mahogany (Swietenia macrophylla) from Venezuela and cedrela (Cedrela odorata) from Costa Rica, we studied the growth layers with variable dimensions of vessels demarcated by marginal axial parenchyma.

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ZHANG Yun-hai, 张运海, 杨皓旻 YANG Hao-min, and 孔晨晖 KONG Chen-hui. "Spectral imaging system on laser scanning confocal microscopy." Optics and Precision Engineering 22, no. 6 (2014): 1446–53. http://dx.doi.org/10.3788/ope.20142206.1446.

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39

Petford, N., G. Davidson, and J. A. Miller. "Pore structure determination using Confocal Scanning Laser Microscopy." Physics and Chemistry of the Earth, Part A: Solid Earth and Geodesy 24, no. 7 (January 1999): 563–67. http://dx.doi.org/10.1016/s1464-1895(99)00080-0.

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40

Hong, Mina, and Guangnan Meng. "Laser Scanning Confocal Microscopy 3D Surface Metrology Applications." Microscopy and Microanalysis 24, S1 (August 2018): 1140–41. http://dx.doi.org/10.1017/s1431927618006189.

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41

Zucker, Robert M., Aparna P. Keshaviah, Owen T. Price, and Jerome M. Goldman. "Confocal Laser Scanning Microscopy of Rat Follicle Development." Journal of Histochemistry & Cytochemistry 48, no. 6 (June 2000): 781–91. http://dx.doi.org/10.1177/002215540004800607.

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42

Lacey, N., F. M. N. Forton, and F. C. Powell. "Demodexquantification methods: limitations of confocal laser scanning microscopy." British Journal of Dermatology 169, no. 1 (July 2013): 212–13. http://dx.doi.org/10.1111/bjd.12280.

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Kumar, Rakesh K., Cheryl C. Chapple, and Neil Hunter. "Improved Double Immunofluorescence for Confocal Laser Scanning Microscopy." Journal of Histochemistry & Cytochemistry 47, no. 9 (September 1999): 1213–17. http://dx.doi.org/10.1177/002215549904700913.

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44

Paddock, Stephen W. "Principles and Practices of Laser Scanning Confocal Microscopy." Molecular Biotechnology 16, no. 2 (2000): 127–50. http://dx.doi.org/10.1385/mb:16:2:127.

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45

Laurent, Michel, Georges Johannin, Nathalie Gilbert, Laurent Lucas, Doris Cassio, Patrice X. Petit, and Anne Fleury. "Power and limits of laser scanning confocal microscopy." Biology of the Cell 80, no. 2-3 (1994): 229–40. http://dx.doi.org/10.1111/j.1768-322x.1994.tb00934.x.

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46

Nutter, Paul W., and C. David Wright. "Resolution Issues in Confocal Magnetooptic Scanning Laser Microscopy." Japanese Journal of Applied Physics 37, Part 1, No. 4B (April 30, 1998): 2245–54. http://dx.doi.org/10.1143/jjap.37.2245.

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47

Rashid, Haroon. "Application of Confocal Laser Scanning Microscopy in Dentistry." Journal of Advanced Microscopy Research 9, no. 4 (December 1, 2014): 245–52. http://dx.doi.org/10.1166/jamr.2014.1217.

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48

Peng, Lee-Cheng, Chien Chou, Chung-Wei Lyu, and Jen-Chuen Hsieh. "Zeeman laser-scanning confocal microscopy in turbid media." Optics Letters 26, no. 6 (March 15, 2001): 349. http://dx.doi.org/10.1364/ol.26.000349.

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49

Gorman, S. P., W. M. Mawhinney, C. G. Adair, and M. Issouckis. "Confocal laser scanning microscopy of peritoneal catheter surfaces." Journal of Medical Microbiology 38, no. 6 (June 1, 1993): 411–17. http://dx.doi.org/10.1099/00222615-38-6-411.

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Bohn, Sebastian, Karsten Sperlich, Thomas Stahnke, Melanie Schünemann, Heinrich Stolz, Rudolf F. Guthoff, and Oliver Stachs. "Multiwavelength confocal laser scanning microscopy of the cornea." Biomedical Optics Express 11, no. 10 (September 18, 2020): 5689. http://dx.doi.org/10.1364/boe.397615.

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