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Academic literature on the topic 'Construct ability'

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Published: 10 March 2023

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Journal articles on the topic "Construct ability"

Ibrahim, Halijah, N. Paul Hear, and Brian Blanksby. "Exploring the General Motor Ability Construct." Perceptual and Motor Skills 113, no. 2 (October 2011): 491–508. http://dx.doi.org/10.2466/03.06.19.25.pms.113.5.491-508.

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LIU, Yuan, and Hong-Yun LIU. "Musical Ability Tests: Construct Extension and Analytical Transition." Advances in Psychological Science 20, no. 8 (June 7, 2013): 1322–28. http://dx.doi.org/10.3724/sp.j.1042.2012.01322.

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김현정. "Defining the construct of second language speaking ability." Studies in Foreign Language Education 31, no. 1 (February 2017): 113–40. http://dx.doi.org/10.16933/sfle.2017.31.1.113.

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Jaehak Chang. "The construct validity of models of L2 reading ability." English Language Teaching 20, no. 4 (December 2008): 355–72. http://dx.doi.org/10.17936/pkelt.2008.20.4.015.

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Oberauer, K., H. M. Süß, R. Schulze, O. Wilhelm, and W. W. Wittmann. "Working memory capacity — facets of a cognitive ability construct." Personality and Individual Differences 29, no. 6 (December 2000): 1017–45. http://dx.doi.org/10.1016/s0191-8869(99)00251-2.

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Titone, Connie, Sharon Sherman, and Ruth Palmer. "Cultivating Student Teachers' Disposition and Ability to Construct Knowledge." Action in Teacher Education 19, no. 4 (January 1998): 76–87. http://dx.doi.org/10.1080/01626620.1998.10462893.

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Kramer, GA, AT Kubiak, and RM Smith. "Construct and predictive validities of the Perceptual Ability Test." Journal of Dental Education 53, no. 2 (February 1989): 119–25. http://dx.doi.org/10.1002/j.0022-0337.1989.53.2.tb02290.x.

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Nafiah, Maratun, Riyadi, and Anton Noornia. "The Effect of Habits of Mind on Ability to Construct a Cognitive Instruments with Computerized Systems." International Journal of Psychosocial Rehabilitation 24, no. 03 (February 19, 2020): 2083–99. http://dx.doi.org/10.37200/ijpr/v24i3/pr200955.

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Taub, Gordon E., B. Grant Hayes, Walter R. Cunningham, and Stephen A. Sivo. "Relative Roles of Cognitive Ability and Practical Intelligence in the Prediction of Success." Psychological Reports 88, no. 3 (June 2001): 931–42. http://dx.doi.org/10.2466/pr0.2001.88.3.931.

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Initial investigations into the construct of practical intelligence have identified a new general factor of practical intelligence ( gp), which is believed to be independent of general cognitive ability. This construct, gp, is also believed to be a better predictor of success than cognitive ability, personality, or any combination of variables independent of gp. The existence of this construct and its independence from Spearman's g is, however, under debate. The purpose of the present study is to investigate both the relationship between gp and g and the relative roles of practical intelligence and cognitive ability in the prediction of success. The participants included 197 college students. Each completed both the Multidimensional Aptitude Battery and Sternberg and Wagner's measure of practical intelligence in academic psychology. The results of structural equation modeling support Sternberg and Wagner's assertion that practical intelligence and general cognitive ability are relatively independent constructs. Results of regression analysis, however, do not support their contention that practical intelligence is related to success after controlling for general cognitive ability. Implications of these results for research and theory on practical intelligence are discussed.

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Collis, Kevin F., Thomas A. Romberg, and Murad E. Jurdak. "A Technique for Assessing Mathematical Problem-Solving Ability." Journal for Research in Mathematics Education 17, no. 3 (May 1986): 206–21. http://dx.doi.org/10.5951/jresematheduc.17.3.0206.

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This report sets out the procedures followed in developing, administering, and scoring a set of mathematical problem-solving superitems and examining their construct validity through a recently developed technique of evaluation associated with a taxonomy of the structure of learned outcomes. Each superitem includes a mathematical situation and a structured set of questions about that situation. To judge whether the response patterns of students to the superitems were interpretable, two questions were raised about the response patterns. For each question, the data strongly support the validity of the underlying theoretical constructs.

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Dissertations / Theses on the topic "Construct ability"

Clenton, Jonathan. "Investigating the construct of productive vocabulary knowledge with Lex30." Thesis, Swansea University, 2010. https://cronfa.swan.ac.uk/Record/cronfa42281.

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This thesis investigates the construct of productive vocabulary knowledge with a productive vocabulary task, Lex30. The task is designed to elicit up to four vocabulary items in response to each of 30 cues. In this way, Lex30 generates a corpus for each subject up to 120 words, which is then categorised according to frequency bands. The test is scored according to the number, or proportion, of infrequent words elicited, with infrequent defined as all items excluding the most frequently occurring 1000 English words. The higher the Lex30 score, then, the more infrequent words that subject has produced in response to the cues. Each corpus generated by Lex30, therefore, offers information about subjects' relative knowledge of infrequent items, although this might only be threshold knowledge. A feature of Lex30 is that it appears to measure productive vocabulary knowledge discretely: it does not activate multiple aspects of language knowledge, and is not context engaging. This feature suggests that we can measure one of the many aspects that are commonly considered to constitute language knowledge, productive vocabulary knowledge, without interference from other aspects of language knowledge. Additionally, Lex30 offers the potential to hypothesize about subjects' relative L2 proficiency in terms of the proportion of infrequent items they provide. To investigate the construct of productive vocabulary with Lex30, this thesis examines, in a principled way, exactly what aspect of language competence it measures, and makes comparisons with other cognate tests. The test has been used in a number of contexts since its introduction; this thesis offers a thorough investigation of its reliability, different versions of the scoring system, the influence cue frequency and of specific cue items, and the mode of task delivery and response. The thesis concludes that Lex30 provides us with a helpful means to understand the construct of productive vocabulary knowledge.

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Christian, Kelly M. "The Construct of Playfulness: Relationships with Adaptive Behaviors, Humor, and Early Play Ability." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1310254723.

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Stritch, David. "A psychometric evaluation of the emotional intelligence ability construct among working adult Australians." Thesis, Stritch, David (2009) A psychometric evaluation of the emotional intelligence ability construct among working adult Australians. Professional Doctorate thesis, Murdoch University, 2009. https://researchrepository.murdoch.edu.au/id/eprint/2468/.

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The broad aim of the research reported here was to evaluate key psychometric issues identified in relation to ability measures of emotional intelligence (EI). To investigate these issues, three studies were conducted including only participants from business and organisational backgrounds. In Study 1, the responses of 231 participants were analysed through a series of confirmatory factor analysis models to evaluate the factor structure of an EI ability measure (MEIS). Results of these analyses were generally consistent with past research, including the observation of non-positive definite matrices for more complex models. Study 2 examined the discriminant validity of the MEIS by comparing the MEIS scores of 147 participants with their responses to the NEO PI-R. Consistent with past research minimal correlations were observed between MEIS scores and NEO PI-R factor scores, indicating that the two instruments measure distinct constructs. There was also good convergence between the two alternative scoring methods for the MEIS, expert- and consensus-based, suggesting that the two scoring methods are comparable when an Australian organisational consensus group is used. As others have suspected (e.g. Palmer, Gignac, Manocha & Stough, 2005) although not investigated, a significant gender by scoring method interaction was observed for the MEIS in Study 2. Study 3 investigated the convergent validity of the MEIS by comparing MEIS scores from 45 participants with their scores on a measure of verbal reasoning. Moderate yet significant correlations were observed indicating that the two measures were related but not to the extent that they were measuring the same construct.

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Wong, Ka Yee Allison. "Construct validity of the test of gross motor development - 2." HKBU Institutional Repository, 2006. http://repository.hkbu.edu.hk/etd_ra/694.

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Penk, Mildred Lotus. "Mental Imagery: The Road to Construct Validity." Thesis, University of North Texas, 1988. https://digital.library.unt.edu/ark:/67531/metadc331872/.

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Internal consistency reliability and validity were established for a new 31 item Imagery Manipulation Scale. Previous attempts to correlate subjectively rated control of visual imagery with tests of spatial ability have been unsuccessful. However, no attempt to construct a subjectively rated control of imagery scale was located which tried to establish internal consistency reliability and both content and construct validity. Further, no research was located in which subjects were requested to rate their imagery ability utilized during the performance of the actual spatial tasks used to try to establish validity. A new scale of subjectively rated control of imagery was devised in which subjects were requested to rate their imagery while solving spatial tasks which involved visualizing the manipulation of geometric forms. Content validity was established by analyzing the transformation involved while solving the spatial problems. Internal consistency reliability for the 31 item scale was established across two samples. Validity was established with the second sample (100 university students: 26 male and 74 female). The task utilized to provide validity could be objectively scored, and was made up of four spatial subtests, which were adapted from the Vandenberg and Kuse Mental Rotations Test, the Kosslyn Directions Test, performed in both the forward and backward direction, and a block task utilized by Snyder. A convergent and discriminant validity analysis established construct validity. Further, the hypotheses of three investigators, Kosslyn, Shepard and his colleagues, and Snyder, were supported by the results of the present investigation, thus substantiating the conclusion that reported control of imagery processing can be operationalized with performance scores on spatial ability tasks.

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Bender, Christine. "ENHANCING LEADERSHIP ABILITY IN FIRST-TIME FRESHMEN THROUGH MENTORSHIP: A GROUNDED THEORY APPROACH." CSUSB ScholarWorks, 2015. https://scholarworks.lib.csusb.edu/etd/163.

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According to the Center for American Progress, women make up 52% of all professional-level jobs, however, they only make up 14.6% of executive officers, 8.1% of top salary earners, and only 4.6% of Fortune 500 CEOs. Considering this disparity in leadership, it brings to question if women are generally taking on leadership throughout their lives. The purpose of this study was to examine how mentorship affects first-time female freshmen’s willingness to take on leadership opportunities. Data were collected from three mentees and three mentors through an initial interest survey, semi-structured personal interviews, and the 3D Wisdom Scale Assessment. Data were analyzed using a ground theory approach which consisted of open, axial, and selective coding; leading to a discursive set of theoretical propositions. Interviews focused on the mentee’s experience through the mentorship program, and the mentor’s perspective of the mentorship program on their mentees. Additionally, the 3D Wisdom Scale developed was utilized to evaluate growth in wisdom dimensions which directly correlated to personal construct corollaries. The findings of the study included: 1) The mentees and mentors expressed growth in their self-esteem and self-confidence. This increase in self-awareness led to a willingness to take risk and face potential failure; 2) Leadership development took place on several levels; 3) The mentees had a realization of the impact of their gender; 4) Two-thirds of the mentees showed a substantial understanding and practice in all three of respective corollaries; and 5) The mentees and mentors displayed general development and growth. The substantive theory that emerged from this grounded theory study was: ‘Through proper mentorship, a first-time female freshman’s’ self-esteem and willingness to take on leadership opportunities will increase.’ Unlike the majority of upperclassmen who have had a mentor, all of the freshmen participating never had a mentor prior to their participation in the program. Mentors played a substantial role in the development of these freshmen and their willingness to take on leadership opportunities. Recommendations for further research include a longitudinal study examining if these freshmen continue to involve themselves in leadership opportunities throughout their college career.

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Parker, Kathy L. "Construct validity of the Differential Ability Scales with a mentally handicapped population : an investigation into the interpretability of cluster scores." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/1019480.

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The purpose of the present study was to investigate the construct validity of the Differential Ability Scales (DAS) with a mentally handicapped population. The DAS is an individually administered, standardized test of intelligence. The stated purposes of the DAS are to provide a composite measure of conceptual reasoning abilities for classification and placement decisions and to provide a reliable profile of relative strengths and weaknesses for diagnostic purposes. With these goals in mind, it follows that this cognitive measure would be used often with mentally handicapped students. The DAS was developed using an hierarchical model based upon exploratory and confirmatory factor analyses. The model assumes that ability measures or subtests will load on a general factor g and will form subfactors at a lower level. The model also assumes that as children get older, the number of subfactors will increase because of development and differentiation of abilities. How mentally handicapped children would fit into this model was the subject of the current research.Using a sample of 100 mildly and moderately handicapped children ages 8 years, 0 months to 17 years, 5 months, confirmatory factor analysis was used to explore the factor structure of the DAS with this population. Three separate models were investigated: Model I, in which a one factor solution was proposed, Model II, in which two factors, Verbal Ability and Nonverbal Ability, were proposed, and Model III, in which three factors, Verbal Ability, Nonverbal Reasoning Ability, and Spatial Ability, as proposed by the test's authors, were investigated. Results of the analyses support the use of a one factor interpretation when using the DAS with mentally handicapped students. In practice, only the broadest score, the General Conceptual Ability Score (GCA), can be interpreted with confidence. Further, case study investigation illustrates the inconsistencies encountered in scoring at the lower end of the norms, as well as in using the outof-level procedure proposed by the test's authors.
Department of Educational Psychology

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Bunin, J. Christopher. "A Study of the Relationship Between Current Event Knowledge and the Ability to Construct a Mental Map of the World." Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/31030.

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This thesis studied the relationship between current event knowledge and the ability to construct a mental map of the world. It was hypothesized that participates with more current event knowledge would demonstrate better mental mapping abilities. The study was designed using two activities recommended for 12th graders by Geography for Life, National Geography Standards 1994, and the theory of spatial familiarity (Kitchen, 1994b; Gale et al., 1990, Golledge & Spector, 1978). One hundred-twenty eight students drawn from two courses offered at Virginia Tech completed a participant profile questionnaire, a current event quiz, drew a map of the world outlining the seven continents, and located and labeled 27 cities on a world map. Using ATLAS GIS the sketch maps and place locations were digitally transformed and scored for accuracy. Descriptive statistics were used to analyze current event knowledge, place location ability, and sketch mapping ability. Using Spearman rank correlation, the relationship between current event knowledge and mental mapping abilities was assessed at a number of levels. Results indicate that participants with a stronger understanding of current events tended to create a more accurate mental map of the world. That is, place location accuracy and drawing accuracy correlated with current event knowledge. However, similar to previous research (Cross, 1987; Helgren, 1983; Muller, 1985) place location knowledge outside of North America and Western Europe was poor. The results of this thesis offer baseline data that can be used for future research to study the effectiveness of the national standards set forth in Geography for Life.
Master of Science

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Ip, Tsang Chui-hing Betty, and 葉鈤翠卿. "The construct validity of the aptitude test for prevocational schools." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1986. http://hub.hku.hk/bib/B3862770X.

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Trippe, David Matthew. "Reexamining the Role of General Cognitive Ability and Specific Abilities in the Prediction of Job Performance Using a Construct-oriented Approach: Not Much More Than g?" Diss., Virginia Tech, 2005. http://hdl.handle.net/10919/27128.

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The purpose of the present study was to attempt to reconcile the seemingly overwhelming body of empirical evidence arguing for the preeminence of general cognitive ability in relation to specific abilities with the general resistance of the majority of Industrial-Organizational psychologists to such a position. The contention of the present study was that the primary evidence used to support the view that specific abilities are of little importance relative to general cognitive ability did not faithfully represent the classic selection model and was based on tenuous assumptions about the operationalizations of general and specific cognitive abilities. By virtue of being defined in un-interpretable terms with respect to content or function, prior operationalizations of specific abilities did not lend themselves to logical and theoretical relationships with job specific job performance. The general thesis of the present study was that if a â construct oriented approachâ that is largely based on this classic selection model were implemented, a composite of psychologically interpretable job related specific abilities would prove equivalent or even superior to general cognitive ability in the prediction of job performance. Results suggest implementation of the construct oriented approach demonstrates potential for the value of this approach with respect to balancing criterion related validity and social equity.
Ph. D.

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Books on the topic "Construct ability"

Towards identifying the C-Test construct. Frankfurt am Main: Peter Lang, 2004.

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Vi-Nhuan, Le, Snow Richard E, and Educational Resources Information Center (U.S.), eds. Construct validation of mathematics achievement: Evidence from interview procedures. Los Angeles, CA: Center for the Study of Evaluation, National Center for Research on Evaluation, Standards, and Student Testing, Graduate School of Education & Information Studies, University of California, Los Angeles, 1999.

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Schwager, Elisa H. An exploration of the construct validity of a leadership behavior rating system. Alexandria, Va: U.S. Army Research Institute for the Behavioral and Social Sciences, 1996.

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Trim, J. L. M. (John Leslie Melville) and Hawkey Roger, eds. Language functions revisited: Theoretical and empirical bases for language construct definition across the ability range. Cambridge: Cambridge University Press, 2011.

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Ludo, Verhoeven, and Jong, John H. A. L. de., eds. The construct of language proficiency: Applications ofpsychological models to language assessment. Amsterdam: John Benjamins Pub. Co, 1992.

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Th, Verhoeven Ludo, and Jong, John H. A. L. de, 1947-, eds. The Construct of language proficiency: Applications of psychological models to language assessment. Amsterdam: John Benjamins Pub. Co., 1992.

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Boldt, Robert F. Multimethod construct validation of the Test of spoken English. Princeton, N.J: Educational Testing Service, 1993.

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Ludo, Verhoeven, and Jong, John H. A. L. de., eds. The Construct of language proficiency: Applications of psychological models to language assessment. Amsterdam: John Benjamins, 1992.

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Yu yan ce shi gou nian xiao du yan jiu: Construct validation in language testing. Beijing Shi: Sichuan da xue chu ban she, 2009.

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Freedle, Roy O. The prediction of TOEFL listening comprehension item difficulty for minitalk passages: Implications for construct validity. Princeton, N.J: Educational Testing Service, 1996.

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Book chapters on the topic "Construct ability"

Angelelli, Claudia V. "Using a rubric to assess translation ability: Defining the construct." In American Translators Association Scholarly Monograph Series, 13–47. Amsterdam: John Benjamins Publishing Company, 2009. http://dx.doi.org/10.1075/ata.xiv.03ang.

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Veraksa, Nikolay, and Aleksander Veraksa. "Developing the Ability of Children to Use and Construct Visual Models." In International Handbook of Early Childhood Education, 1421–37. Dordrecht: Springer Netherlands, 2018. http://dx.doi.org/10.1007/978-94-024-0927-7_73.

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Bachman, Lyle F. "Some construct validity issues in interpreting scores from performance assessments of language ability." In New Perspectives and Issues in Educational Language Policy, 63–90. Amsterdam: John Benjamins Publishing Company, 2001. http://dx.doi.org/10.1075/z.104.07bac.

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Melin, Jeanette, and Leslie R. Pendrill. "The Role of Construct Specification Equations and Entropy in the Measurement of Memory." In Springer Series in Measurement Science and Technology, 269–309. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-07465-3_10.

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AbstractMemory ability, together with many other constructs related to disability and quality of life, is of growing interest in the social sciences, psychology and in health care examinations. This chapter will focus on two elements aiming at understanding, predicting, measuring and quality-assuring constructs with examples from memory measurements: (i) explicit methods for testing theories of the measurement mechanism and establishment of metrological standards and (ii) substantive theories explaining the constructs themselves. Building on entropy as a principal explanatory variable, analogous to its use in thermodynamics and information theory, we demonstrate how more fit-for-purpose and valid memory measurements can be enabled. Firstly, memory task difficulty, extracted from a Rasch psychometric analysis of memory measurements of experimental data such as from the European NeuroMET project, can be explained with a construct specification equation (CSE). Based on that understanding, the CSE can facilitate the establishment of objective and scalable units through the generation of novel certified reference “materials” for metrological traceability and comparability. These formulations of CSEs can also guide how best to compose new memory metrics, through a judicious choice of items from various legacy tests guided by entropy-based equivalence, which opens up opportunities for formulating new, less onerous but more sensitive and representative tests. Finally, we propose and demonstrate how to formulate CSEs for person ability, correlated statistically and clinically with sets of biomarkers, that can be a means of providing diagnostic information to enhance clinical decisions and targeted interventions.

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Stenner, A. Jackson. "Theory Referenced Measurement: Combining Substantive Theory and the Rasch Model." In Explanatory Models, Unit Standards, and Personalized Learning in Educational Measurement, 121–25. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-3747-7_9.

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AbstractA construct theory is the story we tell about what it means to move up and down the scale for a variable of interest (e.g., temperature, reading ability, short term memory). Why is it, for example, that items are ordered as they are on the item map? The story evolves as knowledge regarding the construct increases. We call both the process and the product of this evolutionary unfolding "construct definition" (Stenner et al., Journal of Educational Measurement 20:305–316, 1983). Advanced stages of construct definition are characterized by calibration equations (or specification equations) that operationalize and formalize a construct theory. These equations, make point predictions about item behavior or item ensemble distributions. The more closely theoretical calibrations coincide with empirical item difficulties, the more useful the construct theory and the more interesting the story. Twenty-five years of experience in developing the Lexile Framework for Reading enable us to distinguish five stages of thinking. Each subsequent stage can be characterized by an increasingly sophisticated use of substantive theory. Evidence that a construct theory and its associated technologies have reached a given stage or level can be found in the artifacts, instruments, and social networks that are realized at each level.

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Stenner, A. Jackson, and Richard J. Rohlf. "Construct Definition Methodology and Generalizability Theory Applied to Career Education Measurement." In Explanatory Models, Unit Standards, and Personalized Learning in Educational Measurement, 17–30. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-3747-7_2.

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AbstractThe field of career education measurement is in disarray. Evidence mounts that today’s career education instruments are verbal ability measures in disguise. A plethora of trait names such as career maturity, career development, career planning, career awareness, and career decision making have, in the last decade, appeared as labels to scales comprised of multiple choice items. Many of these scales appear to be measuring similar underlying traits and certainly the labels have a similar sound or “jingle” to them. Other scale names are attached to clusters of items that appear to measure different traits and at first glance appear deserving of their unique trait names, e.g., occupational information, resources for exploration, work conditions, personal economics. The items of these scales look different and the labels correspondingly are dissimilar or have a different “jangle” to them.

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Liu, Yubo, Yihua Luo, Qiaoming Deng, and Xuanxing Zhou. "Exploration of Campus Layout Based on Generative Adversarial Network." In Proceedings of the 2020 DigitalFUTURES, 169–78. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-33-4400-6_16.

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AbstractThis paper aims to explore the idea and method of using deep learning with a small amount sample to realize campus layout generation. From the perspective of the architect, we construct two small amount sample campus layout data sets through artificial screening with the preference of the specific architects. These data sets are used to train the ability of Pix2Pix model to automatically generate the campus layout under the condition of the given campus boundary and surrounding roads. Through the analysis of the experimental results, this paper finds that under the premise of effective screening of the collected samples, even using a small amount sample data set for deep learning can achieve a good result.

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Bond, Melissa, and Nina Bergdahl. "Student Engagement in Open, Distance, and Digital Education." In Handbook of Open, Distance and Digital Education, 1–16. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-0351-9_79-1.

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AbstractEngaging students in their learning, and within their learning community, is a key goal of educators. However, ongoing discussions about its nature, conceptualization, and measurement have led to a diffusion of the concept’s understanding, and ability to apply it within both research and practice. This chapter draws on theoretical and empirical primary and secondary ODDE research, and provides an overview of student engagement and disengagement, particularly as they relate to educational technology. The four dimensions of behavioral, affective/emotional, cognitive, and social (dis-)engagement are presented, alongside example indicators. In addition, a bioecological model of student engagement is explored with explicit links to digital learning. The chapter concludes by providing open questions and directions for future research, including further emphasis and exploration needed on the role of social engagement in ODDE contexts, as well as disengagement as a separate construct.

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Bond, Melissa, and Nina Bergdahl. "Student Engagement in Open, Distance, and Digital Education." In Handbook of Open, Distance and Digital Education, 1309–24. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-2080-6_79.

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Chergui, Akram, Nicolas Beraud, Frédéric Vignat, and François Villeneuve. "Finite Element Modeling and Validation of Metal Deposition in Wire Arc Additive Manufacturing." In Lecture Notes in Mechanical Engineering, 61–66. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-70566-4_11.

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AbstractWire arc additive manufacturing allows the production of metallic parts by depositing beads of weld metal using arc-welding technologies. This low-cost additive manufacturing technology has the ability to manufacture large-scale parts at a high deposition rate. However, the quality of the obtained parts is greatly affected by the various thermal phenomena present during the manufacturing process. Numerical simulation remains an effective tool for studying such phenomena. In this work, a new finite element technique is proposed in order to model metal deposition in WAAM process. This technique allows to gradually construct the mesh representing the deposited regions along the deposition path. The heat source model proposed by Goldak is adapted and combined with the proposed metal deposition technique taking into account the energy distribution between filler material and the molten pool. The effectiveness of the proposed method is validated by series of experiments, of which an example is detailed in this paper.

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Conference papers on the topic "Construct ability"

Yih, Jeng-Ming. "Construct ability indicators of learning Algebra." In 2016 International Conference on Applied System Innovation (ICASI). IEEE, 2016. http://dx.doi.org/10.1109/icasi.2016.7539919.

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Speirs, J. Caleb, William N. Ferm Jr., MacKenzie R. Stetzer, and Beth A. Lindsey. "Probing Student Ability to Construct Reasoning Chains: A New Methodology." In 2016 Physics Education Research Conference. American Association of Physics Teachers, 2016. http://dx.doi.org/10.1119/perc.2016.pr.077.

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Stefanone, Michael A., Alaina Iacobucci, and Elena Svetieva. "Developing the Network Awareness Construct: Evidence Supporting the Ability to Understand Social Situations." In 2016 49th Hawaii International Conference on System Sciences (HICSS). IEEE, 2016. http://dx.doi.org/10.1109/hicss.2016.256.

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Jia, Guangcai. "The Cultivation of College Students' Ability to Construct Mathematical Model in Campus Education." In IPEC 2021: 2021 2nd Asia-Pacific Conference on Image Processing, Electronics and Computers. New York, NY, USA: ACM, 2021. http://dx.doi.org/10.1145/3452446.3452684.

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Mertasari, Ni Made Sri, and I. Made Candiasa. "Multitrait-Multimethod Technique for Construct Validity: A Case Study for Instruments of Critical Thinking Ability and Creative Thinking Ability in Programming Course." In Proceedings of the 4th International Conference on Vocational Education and Technology, IConVET 2021, 27 November 2021, Singaraja, Bali, Indonesia. EAI, 2022. http://dx.doi.org/10.4108/eai.27-11-2021.2315544.

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Huiyu, Zhao, Yan Ming, and Yin Boming. "Application of grey analytic hierarchy process to construct the middle-school student innovation ability index system." In 2013 IEEE International Conference on Grey Systems and Intelligent Services (GSIS). IEEE, 2013. http://dx.doi.org/10.1109/gsis.2013.6714773.

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Stavrou, Eliana. "A situation-aware user interface to assess users' ability to construct strong passwords: A conceptual architecture." In 2017 International Conference On Cyber Situational Awareness, Data Analytics And Assessment (Cyber SA). IEEE, 2017. http://dx.doi.org/10.1109/cybersa.2017.8073385.

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Fisher, Matthew B., Nicole Söegaard, David R. Steinberg, and Robert L. Mauck. "Trajectory-Based Tissue Engineering for Cartilage Repair: Correlation Between Maturation Rate and Integration Capacity." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14572.

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Given the limitations of current surgical approaches to treat articular cartilage injuries, tissue engineering (TE) approaches have been aggressively pursued over the past two decades. Although biochemical and biomechanical properties on the order of the native tissue have been achieved (1–5), several in-vitro and in-vivo studies indicate that increased tissue maturity may limit the ability of engineered constructs to remodel and integrate with surrounding cartilage, although results are highly variable (2, 6–8). Thus, “static” measures of construct maturity (e.g. compressive modulus) upon implantation may not be the best indicators of in-vivo success, which likely requires implanted TE constructs to mature, remodel, and integrate with the host over time to achieve optimal results. We recently introduced the concept of “trajectory-based” tissue engineering (TB-TE), which is based on the general hypothesis that time-dependent increases in construct maturation in-vitro prior to implantation (i.e. positive rates) may provide a better predictor of in-vivo success (9). As a first step in evaluating this concept, in the current study we hypothesized that time-dependent increases in equilibrium modulus (a metric of growth) would be correlated to ability of constructs to integrate to cartilage using an in-vitro assay. To test this hypothesis, the current objective was to determine and model the time course of maturation of TE constructs during in-vitro culture and to assess the ability of these constructs to integrate to cartilage at various points during their maturation.

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Sejati, Andri Estining, La Ode Amaluddin, Desi N. Hidayati, and Sitti Kasmiati. "The Effect of Outdoor Study on the Geography Scientific Paper Writing Ability to Construct Student Character in Senior High School." In 5th SEA-DR (South East Asia Development Research) International Conference 2017 (SEADRIC 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/seadric-17.2017.22.

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Farrell, Megan J., Eric S. Comeau, and Robert L. Mauck. "Dynamic Culture Improves Mechanical Functionality of MSC-Laden Tissue Engineered Constructs in a Depth-Dependent Manner." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53442.

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Limitations associated with the use of autologous chondrocytes (CH) for cartilage tissue engineering beget the need for alternative cell sources. Mesenchymal stem cells (MSC) are clinically attractive due to their ability to undergo chondrogenesis in three-dimensional culture [1,2]; however, when compared to CH, MSC fail to develop functional equivalence [2,3]. We have previously shown a marked depth-dependence in local equilibrium modulus of MSC-laden gels, with the superficial zones (where maximal media exchange occurs) considerably stiffer than regions removed from nutrient supply (center and bottom of construct); less dramatic depth-dependence was observed in CH-laden gels [4]. Similarly, other studies have shown depth-dependent properties in CH-laden gels with the construct edge generally stiffer than the center [5]. Given this apparent influence of nutrient supply, the objective of the current study was to assess the impact of dynamic culture (via orbital shaking) on the development of depth-dependent mechanical properties in both MSC and CH-laden hydrogels. Furthermore, we assessed cell viability and matrix content throughout the construct depth to determine the mechanism by which this depth-dependency arises. We hypothesized that improved nutrient transport would reduce construct inhomogeneity (particularly for MSC-laden constructs) and improve bulk mechanical properties.

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Reports on the topic "Construct ability"

Roberts, Scott Alan, and Mark Edward Ferraro. CABS Milestone I3 Report: Demonstration of ability to construct 3D meshes of electrodes using reconstructions from micro-tomography. Office of Scientific and Technical Information (OSTI), December 2016. http://dx.doi.org/10.2172/1505390.

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Palmer, Guy H., Eugene Pipano, Terry F. McElwain, Varda Shkap, and Donald P. Knowles, Jr. Development of a Multivalent ISCOM Vaccine against Anaplasmosis. United States Department of Agriculture, July 1993. http://dx.doi.org/10.32747/1993.7568763.bard.

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Anaplasmosis is an arthropod+borne disease of cattle caused by the rickettsia Anaplasma marginale and an impediment to efficient production of healthy livestock in both Israel and the United States. Our research focuses on development of a recombinant membrane surface protein (MSP) immunogen to replace current vaccines derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Briefly, we accomplished the following in our BARD supported research: i) characterization of the intramolecular and intermolecular relationships of the native Major Surface Proteins (MSP) in the outer membrane; ii) expression, purification, and epitope characterization of the recombinant MSP-2, MSP-3, MSP-4, and MSP-5 proteins required to construct the recombinant ISCOM; iii) demonstration that the outer membrane-Quil A induces CD4+ T lymphocytes specific for the outer membrane polypeptides; iv) identification of CD4+ T lymphocytes that recognize outer membrane polypeptide epitopes conserved among other wise antigenically distinct strains; v) determination that immunization with the outer membrane-Quil A construct does not affect the ability of ticks to acquire or transmit A. marginale; and vi) demonstration that the outer membrane-Quil A construct induces complete protection against rickettsemia upon homologous challenge and significant protection against challenge with antigenically distinct strains, including tick transmission. Importantly, the level of protection against homologous challenge in the MSP vaccinates was comparable to that induced by live blood-based vaccines and demonstrates that development of a new generation of vaccines is feasible.

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Tillett, Will, and Oliver Jones. Improving Rural Sanitation in Challenging Contexts. Institute of Development Studies (IDS), October 2021. http://dx.doi.org/10.19088/slh.2021.020.

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Of the two billion people worldwide lacking access to at least basic sanitation, seven out of ten live in rural areas. Progress has been made on increasing rural sanitation and access levels are rising, but barriers remain in reaching the ‘last mile’ or some 10 to 20 per cent of the population who live in the most challenging contexts. The factors affecting the ability of households to construct and use toilets, as well as the challenges sanitation programmes face in reaching specific groups, are highly diverse. Applying one-size fits all approaches has been proven not to work; therefore, we need more nuanced, adapted, and targeted approaches to capture the universality element of the Sustainable Development Goals (SDGs) and ensure that no one is left behind. However, we recognise that challenges can be persistent and there are limited documented examples of how to overcome these challenges at scale. The Sanitation Learning Hub, UNICEF, and WaterAid commissioned this study to map rural sanitation approaches in challenging contexts and the guidance currently being used, drawing out emerging experiences and lessons. It involved key informant interviews (KIIs) with 44 interviewees, and consulting over 180 documented resources. This Learning Brief provides an overview of the study findings.

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Tillett, Will, and Oliver Jones. ‘Improving Rural Sanitation in Challenging Contexts’ Sanitation Learning Hub Learning Brief 8. The Sanitation Learning Hub, Institute of Development Studies, March 2021. http://dx.doi.org/10.19088/slh.2021.006.

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Osadchyi, Viacheslav V., Hanna B. Varina, Kateryna P. Osadcha, Olha V. Kovalova, Valentyna V. Voloshyna, Oleksii V. Sysoiev, and Mariya P. Shyshkina. The use of augmented reality technologies in the development of emotional intelligence of future specialists of socionomic professions under the conditions of adaptive learning. CEUR Workshop Proceedings, July 2020. http://dx.doi.org/10.31812/123456789/4633.

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In modern conditions, innovative augmented reality technologies are actively developing, which are widespread in many areas of human activity. Introduction of advanced developments in the process of professional training of future specialists of socionomic professions in the conditions of adaptive training, contributes to the implementation of the principles of a personalized approach and increase the overall level of competitiveness. The relevant scientific article is devoted to the theoretical and empirical analysis result of conducting a psychodiagnostic study on an innovative computer complex HC-psychotest. of the features of the implementation of augmented reality technologies in the construct of traditional psychological and pedagogical support aimed at the development of emotional intelligence of the future specialist. The interdisciplinary approach was used while carrying out the research work at the expense of the general fund of the state budget: “Adaptive system for individualization and personalization of professional training of future specialists in the conditions of blended learning”. A comprehensive study of the implementation of traditional psychological-pedagogical and innovative augmented reality technologies was conducted in the framework of scientific cooperation of STEAM-Laboratory, Laboratory of Psychophysiological Research and Laboratory of Psychology of Health in Bogdan Khmelnitsky Melitopol State Pedagogical University. The theoretical analysis considers the structural model of emotional intelligence of the future specialist of socionomic professions, which is represented by two structural components: intrapersonal construct of emotional intelligence and interpersonal construct of emotional intelligence. Each component mediates the inherent emotional intelligence of interpretive, regulatory, adaptive, stress-protective and activating functions. The algorithm of the empirical block of research is presented by two stages: ascertaining and forming research. According to the results of the statement, low indicators were found on most scales, reflecting the general level of emotional intelligence development of future specialists, actualizing the need to find and implement effective measures for the development of emotional intelligence components in modern higher education and taking into account information development and digitalization. As part of the formative stage of the research implementation, a comprehensive program “Development of emotional intelligence of future professionals” was tested, which integrated traditional psychological and pedagogical technologies and innovative augmented reality technologies. This program is designed for 24 hours, 6 thematic classes of 4 hours. According to the results of a comprehensive ascertaining and shaping research, the effectiveness of the influence of augmented reality technologies on the general index of emotional intelligence is proved. The step-by-step model of integration of augmented reality components influencing the ability to analyze, understand and regulate emotional states into a complex program of emotional intelligence development is demonstrated. According to the results of the formative study, there is a dominance of high indicators of the following components: intrapersonal (50%), interpersonal (53.3%). Thus, we can say that intrapersonal and interpersonal emotional intelligence together involve the actualization of various cognitive processes and skills, and are related to each other. Empirical data were obtained as a

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Manulis-Sasson, Shulamit, Christine D. Smart, Isaac Barash, Laura Chalupowicz, Guido Sessa, and Thomas J. Burr. Clavibacter michiganensis subsp. michiganensis-tomato interactions: expression and function of virulence factors, plant defense responses and pathogen movement. United States Department of Agriculture, February 2015. http://dx.doi.org/10.32747/2015.7594405.bard.

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Clavibactermichiganensissubsp. michiganensis(Cmm), the causal agent of bacterial wilt and canker of tomato, is the most destructive bacterial disease of tomato causing substantial economic losses in Israel, the U.S.A. and worldwide. The goal of the project was to unravel the molecular strategies that allow Cmm, a Gram-positive bacterium, to develop a successful infection in tomato. The genome of Cmm contains numerous genes encoding for extracellular serine proteases and cell wall degrading enzymes. The first objective was to elucidate the role of secreted serine proteases in Cmm virulence. Mutants of nine genes encoding serine proteases of 3 different families were tested for their ability to induce wilting, when tomato stems were puncture-inoculated, as compared to blisters formation on leaves, when plants were spray-inoculated. All the mutants showed reduction in wilting and blister formation as compared to the wild type. The chpCmutant displayed the highest reduction, implicating its major role in symptom development. Five mutants of cell wall degrading enzymes and additional genes (i.e. perforin and sortase) caused wilting but were impaired in their ability to form blisters on leaves. These results suggest that Cmm differentially expressed virulence genes according to the site of penetration. Furthermore, we isolated and characterized two Cmmtranscriptional activators, Vatr1 and Vatr2 that regulate the expression of virulence factors, membrane and secreted proteins. The second objective was to determine the effect of bacterial virulence genes on movement of Cmm in tomato plants and identify the routes by which the pathogen contaminates seeds. Using a GFP-labeledCmm we could demonstrate that Cmm extensively colonizes the lumen of xylem vessels and preferentially attaches to spiral secondary wall thickening of the protoxylem and formed biofilm-like structures composed of large bacterial aggregates. Our findings suggest that virulence factors located on the chp/tomAPAI or the plasmids are required for effective movement of the pathogen in tomato and for the formation of cellular aggregates. We constructed a transposon plasmid that can be stably integrated into Cmm chromosome and express GFP, in order to follow movement to the seeds. Field strains from New York that were stably transformed with this construct, could not only access seeds systemically through the xylem, but also externally through tomato fruit lesions, which harbored high intra-and intercellular populations. Active movement and expansion of bacteria into the fruit mesocarp and nearby xylem vessels followed, once the fruit began to ripen. These results highlight the ability of Cmm to invade tomato fruit and seed through multiple entry routes. The third objective was to assess correlation between disease severity and expression levels of Cmm virulence genes and tomato defense genes. The effect of plant age on expression of tomato defense related proteins during Cmm infection was analyzed by qRT-PCR. Five genes out of eleven showed high induction at early stages of infection of plants with 19/20 leaves compared to young plants bearing 7/8 leaves. Previous results showed that Cmm virulence genes were expressed at early stages of infection in young plants compared to older plants. Results of this study suggest that Cmm virulence genes may suppress expression of tomato defense-related genes in young plants allowing effective disease development. The possibility that chpCis involved in suppression of tomato defense genes is currently under investigation by measuring the transcript level of several PR proteins, detected previously in our proteomics study. The fourth objective was to define genome location and stability of virulence genes in Cmm strains. New York isolates were compared to Israeli, Serbian, and NCPPB382 strains. The plasmid profiles of New York isolates were diverse and differed from both Israeli and Serbian strains. PCR analysis indicated that the presence of putative pathogenicity genes varied between isolates and highlighted the ephemeral nature of pathogenicity genes in field populations of Cmm. Results of this project significantly contributed to the understanding of Cmm virulence, its movement within tomato xylem or externally into the seeds, the role of serine proteases in disease development and initiated research on global regulation of Cmm virulence. These results form a basis for developing new strategies to combat wilt and canker disease of tomato.

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Palmer, Guy, Varda Shkap, Wendy Brown, and Thea Molad. Control of bovine anaplasmosis: cytokine enhancement of vaccine efficacy. United States Department of Agriculture, March 2007. http://dx.doi.org/10.32747/2007.7695879.bard.

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Anaplasmosis an arthropod-born disease of cattle caused by the rickettsia Anaplasma marginale and is an impediment to efficient production of healthy livestock in both Israel and the United States. Currently the only effective vaccines are derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Consequently development of a safe, effective vaccine is a high priority. In this collaborative project we focused on two approaches to vaccine development. The first focused o n improving antigen delivery to livestock and specifically examined how DNA vaccines could be improved to enhance priming and expansion of the immune response. This research resulted in development and testing of two novel vaccine delivery systems--one that targeted antigen spread among dendritic cells (the key cell in priming immune responses and a follow-on construct that also specifically targeted antigen to the endosomal-lysosomal compartment the processing organelle within the dendritic cell that directs vaccine antigen to the MHC class ll-CD4* T cell priming pathway). The optimized construct targeting vaccine antigen to the dendritic cell MHC class II pathway was tested for ability to prime A. marginale specific immune responses in outbred cattle. The results demonstrated both statistically significant effects of priming with a single immunization, continued expansion of the primary immune response including development of high affinity lgG antibodies and rapid recall of the memory response following antigen challenge. This portion of the study represented a significant advance in vaccine delivery for livestock. Importantly the impact of these studies is not limited to A. marginale a s the targeting motifs are optimized for cattle and can be adapted to other cattle vaccinations by inserting a relevant pathogen-specific antigen. The second approach (which represented an addition to the project for which approval was requested as part of the first annual report) was a comparative approach between A . marginale and the Israel A . centrale vaccines train. This addition was requested as studies on Major Surface Protein( MSP)- 2 have shown that this antigen is highly antigenically variable and presented solely as a "static vaccine" antigen does not give cross-strain immunity. In contrast A. . centrale is an effective vaccine which Kimron Veterinary institute has used in the field in Israel for over 50 years. Taking advantage of this expertise, a broad comparison of wild type A. marginale and vaccine strain was initiated. These studies revealed three primary findings: i) use of the vaccine is associated with superinfection, but absence of clinical disease upon superinfection with A. marginale; ii) the A. centrale vaccine strain is not only less virulent but transmission in competent in Dermacentor spp. ticks; and iii) some but not all MSPs are conserved in basic orthologous structure but there are significant polymorphisms among the strains. These studies clearly indicated that there are statistically significant differences in biology (virulence and transmission) and provide a clear path for mapping of biology with the genomes. Based on these findings, we initiated complete genome sequencing of the Israel vaccine strain (although not currently funded by BARD) and plant to proceed with a comparative genomics approach using already sequenced wild-type A. marginale. These findings and ongoing collaborative research tie together filed vaccine experience with new genomic data, providing a new approach to vaccine development against a complex pathogen.

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Alfano, James, Isaac Barash, Thomas Clemente, Paul E. Staswick, Guido Sessa, and Shulamit Manulis. Elucidating the Functions of Type III Effectors from Necrogenic and Tumorigenic Bacterial Pathogens. United States Department of Agriculture, January 2010. http://dx.doi.org/10.32747/2010.7592638.bard.

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Many phytopathogenic bacteria use a type III protein secretion system (T3SS) to inject type III effectors into plant cells. In the experiments supported by this one-year feasibility study we investigated type III effector function in plants by using two contrasting bacterial pathogens: Pseudomonas syringae pv. tomato, a necrotrophic pathogen and Pantoea agglomerans, a tumorigenic pathogen. The objectives are listed below along with our major conclusions, achievements, and implications for science and agriculture. Objective 1: Compare Pseudomonas syringae and Pantoea agglomerans type III effectors in established assays to test the extent that they can suppress innate immunity and incite tumorigenesis. We tested P. agglomerans type III effectors in several innate immunity suppression assays and in several instances these effectors were capable of suppressing plant immunity, outputs that are suppressed by P. syringae effectors. Interestingly, several P. syringae effectors were able to complement gall production to a P. agglomerans pthGmutant. These results suggest that even though the disease symptoms of these pathogens are dramatically different, their type III effectors may function similarly. Objective 2: Construct P. syringae mutants in different combinations of type III-related DNA clusters to reduce type III effector redundancy. To determine their involvement in pathogenicity we constructed mutants that lack individual and multiple type III-related DNA clusters using a Flprecombinase-mediated mutagenesis strategy. The majority of single effector mutants in DC3000 have weak pathogenicity phenotypes most likely due to functional redundancy of effectors. Supporting this idea, Poly-DNAcluster deletion mutants were more significantly reduced in their ability to cause disease. Because these mutants have less functional redundancy of type III effectors, they should help identify P. syringae and P. agglomerans effectors that contribute more significantly to virulence. Objective 3: Determine the extent that P. syringae and P. agglomerans type III effectors alter hormone levels in plants. Inhibition of auxin polar transport by 2,3,5-triiodobenzoic acid (TIBA) completely prevented gall formation by P. agglomerans pv. gypsophilae in gypsophila cuttings. This result supported the hypothesis that auxin and presumably cytokinins of plant origin, rather than the IAA and cytokinins secreted by the pathogen, are mandatory for gall formation. Transgenic tobacco with pthGshowed various phenotypic traits that suggest manipulation of auxin metabolism. Moreover, the auxin levels in pthGtransgenic tobacco lines was 2-4 times higher than the control plants. External addition of auxin or cytokinins could modify the gall size in gypsophila cuttings inoculated with pthGmutant (PagMx27), but not with other type III effectors. We are currently determining hormone levels in transgenic plants expressing different type III effectors. Objective 4: Determine whether the P. agglomerans effectors HsvG/B act as transcriptional activators in plants. The P. agglomerans type III effectors HsvG and HsvB localize to the nucleus of host and nonhost plants and act as transcription activators in yeast. Three sites of adjacent arginine and lysine in HsvG and HsvB were suspected to act as Nuclear localization signals (NLS) domains. A nuclear import assay indicated two of the three putative NLS domains were functional NLSs in yeast. These were shown to be active in plants by fusing HsvG and HsvB to YFP. localization to the nucleus was dependent on these NLS domains. These achievements indicate that our research plan is feasible and suggest that type III effectors suppress innate immunity and modulate plant hormones. This information has the potential to be exploited to improve disease resistance in agricultural crops.

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Fluhr, Robert, and Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7697123.bard.

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Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TIR LECTIN WOUNDRESPONSIVE1). The TLW1 gene encodes a protein with two domains: a TIR domain linked to a lectin-containing domain. Our specific aim in this proposal was to examine the ramifications of the TL1-glycan interaction by; A) The functional characterization of TL1 activity in the context of plant wound response and B) Examine the hypothesis that wounding induced specific polysaccharides and examine them as candidates for TL-1 interactive glycan compounds. The Weizmann group showed TLW1 transcripts are rapidly induced by wounding in a JA-independent pathway and T-DNA-tagged tlw1 mutants that lack TLW1 transcripts, fail to initiate the full systemic wound response. Transcriptome methodology analysis was set up and transcriptome analyses indicates a two-fold reduced level of JA-responsive but not JA-independent transcripts. The TIR domain of TLW1 was found to interact directly with the KAT2/PED1 gene product responsible for the final b-oxidation steps in peroxisomal-basedJA biosynthesis. To identify potential binding target(s) of TL1 in plant wound response, the CCRC group first expressed recombinant TL1 in bacterial cells and optimized conditions for the protein expression. TL1 was most highly expressed in ArcticExpress cell line. Different types of extraction buffers and extraction methods were used to prepare plant extracts for TL1 binding assay. Optimized condition for glycan labeling was determined, and 2-aminobenzamide was used to label plant extracts. Sensitivity of MALDI and LC-MS using standard glycans. THAP (2,4,6- Trihydroxyacetophenone) showed minimal background peaks at positive mode of MALDI, however, it was insensitive with a minimum detection level of 100 ng. Using LC-MS, sensitivity was highly increased enough to detect 30 pmol concentration. However, patterns of total glycans displayed no significant difference between different extraction conditions when samples were separated with Dionex ICS-2000 ion chromatography system. Transgenic plants over-expressing lectin domains were generated to obtain active lectin domain in plant cells. Insertion of the overexpression construct into the plant genome was confirmed by antibiotic selection and genomic DNA PCR. However, RT-PCR analysis was not able to detect increased level of the transcripts. Binding ability of azelaic acid to recombinant TL1. Azelaic acid was detected in GST-TL1 elution fraction, however, DHB matrix has the same mass in background signals, which needs to be further tested on other matrices. The major findings showed the importance of TLW1 in regulating wound response. The findings demonstrate completely novel and unexpected TIR domain interactions and reveal a control nexus and mechanism that contributes to the propagation of wound responses in Arabidopsis. The implications are to our understanding of the function of TIR domains and to the notion that early molecular events occur systemically within minutes of a plant sustaining a wound. A WEB site (http://genome.weizmann.ac.il/hormonometer/) was set up that enables scientists to interact with a collated plant hormone database.

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Morin, Shai, Gregory Walker, Linda Walling, and Asaph Aharoni. Identifying Arabidopsis thaliana Defense Genes to Phloem-feeding Insects. United States Department of Agriculture, February 2013. http://dx.doi.org/10.32747/2013.7699836.bard.

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The whitefly (Bemisia tabaci) is a serious agricultural pest that afflicts a wide variety of ornamental and vegetable crop species. To enable survival on a great diversity of host plants, whiteflies must have the ability to avoid or detoxify numerous different plant defensive chemicals. Such toxins include a group of insect-deterrent molecules called glucosinolates (GSs), which also provide the pungent taste of Brassica vegetables such as radish and cabbage. In our BARD grant, we used the whitefly B. tabaci and Arabidopsis (a Brassica plant model) defense mutants and transgenic lines, to gain comprehensive understanding both on plant defense pathways against whiteflies and whitefly defense strategies against plants. Our major focus was on GSs. We produced transgenic Arabidopsis plants accumulating high levels of GSs. At the first step, we examined how exposure to high levels of GSs affects decision making and performance of whiteflies when provided plants with normal levels or high levels of GSs. Our major conclusions can be divided into three: (I) exposure to plants accumulating high levels of GSs, negatively affected the performance of both whitefly adult females and immature; (II) whitefly adult females are likely to be capable of sensing different levels of GSs in their host plants and are able to choose, for oviposition, the host plant on which their offspring survive and develop better (preference-performance relationship); (III) the dual presence of plants with normal levels and high levels of GSs, confused whitefly adult females, and led to difficulties in making a choice between the different host plants. These findings have an applicative perspective. Whiteflies are known as a serious pest of Brassica cropping systems. If the differences found here on adjacent small plants translate to field situations, intercropping with closely-related Brassica cultivars could negatively influence whitefly population build-up. At the second step, we characterized the defensive mechanisms whiteflies use to detoxify GSs and other plant toxins. We identified five detoxification genes, which can be considered as putative "key" general induced detoxifiers because their expression-levels responded to several unrelated plant toxic compounds. This knowledge is currently used (using new funding) to develop a new technology that will allow the production of pestresistant crops capable of protecting themselves from whiteflies by silencing insect detoxification genes without which successful host utilization can not occur. Finally, we made an effort to identify defense genes that deter whitefly performance, by infesting with whiteflies, wild-type and defense mutated Arabidopsis plants. The infested plants were used to construct deep-sequencing expression libraries. The 30- 50 million sequence reads per library, provide an unbiased and quantitative assessment of gene expression and contain sequences from both Arabidopsis and whiteflies. Therefore, the libraries give us sequence data that can be mined for both the plant and insect gene expression responses. An intensive analysis of these datasets is underway. We also conducted electrical penetration graph (EPG) recordings of whiteflies feeding on Arabidopsis wild-type and defense mutant plants in order to determine the time-point and feeding behavior in which plant-defense genes are expressed. We are in the process of analyzing the recordings and calculating 125 feeding behavior parameters for each whitefly. From the analyses conducted so far we conclude that the Arabidopsis defense mutants do not affect adult feeding behavior in the same manner that they affect immatures development. Analysis of the immatures feeding behavior is not yet completed, but if it shows the same disconnect between feeding behavior data and developmental rate data, we would conclude that the differences in the defense mutants are due to a qualitative effect based on the chemical constituency of the phloem sap.

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Academic literature on the topic 'Construct ability'

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