Relevant bibliographies by topics / CONSTRUCTION OF LIBRARIES

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'CONSTRUCTION OF LIBRARIES'

Author: Grafiati
Published: 24 April 2022

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Journal articles on the topic "CONSTRUCTION OF LIBRARIES"

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Zhao, Chen. "Study on the Reform and Development of University Library in China." Advanced Materials Research 271-273 (July 2011): 1381–85. http://dx.doi.org/10.4028/www.scientific.net/amr.271-273.1381.

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The analysis of present situation of university libraries in China shows that the literature resources and the readers are all diversified. The urge demand for the libraries includes fast and efficient services and group work. The university libraries are characterized as knowledge carriers, information servers and literature collectors. The libraries should be reformed to provide diversified resources, precise services and easy acquisition of literatures. The reform of university libraries should follow the way from library planning, resources construction to literature sharing. The construction of library houses, literature resources and librarian teams is analyzed. The reform will force the libraries to be specialized in providing literature services. The libraries will continue to function as a typical cultural area in university campus.
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Szeberényi, Jozséf. "Test: Construction of genomic libraries." Biochemistry and Molecular Biology Education 33, no. 2 (November 3, 2006): 135–37. http://dx.doi.org/10.1002/bmb.2005.494033022438.

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Huang, Yong Xiang. "Discussion on Special Library in Department Serving to Discipline Construction." Applied Mechanics and Materials 411-414 (September 2013): 2823–26. http://dx.doi.org/10.4028/www.scientific.net/amm.411-414.2823.

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The special libraries in departments with the comprehensive library of university constitute together a multi-level, multi-faceted and multi-type college literature resources sharing system. The special libraries in department could be play an unique role for discipline construction and join in discipline construction through providing several services including literature and information service, special works service and date processing. In the construction of special library in department, special attention must be paid to the purchase of professional books, the reformation and innovation of service mode to highlight the construction of discipline, and also the enhancing professional qualities of librarians.
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Stauffer. "Libraries Are the Homes of Books: Whiteness in the Construction of School Libraries." Libraries: Culture, History, and Society 1, no. 2 (2017): 194. http://dx.doi.org/10.5325/libraries.1.2.0194.

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Cao, Gaohui, Mengli Liang, and Xuguang Li. "How to make the library smart? The conceptualization of the smart library." Electronic Library 36, no. 5 (October 1, 2018): 811–25. http://dx.doi.org/10.1108/el-11-2017-0248.

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Purpose This paper aims to clearly conceptualize the idea of the smart library and propose a holistic approach to building smart libraries, in accordance with recent practices and state-of-the-art technologies. Design/methodology/approach Drawing on an extensive review of existing literature and practice about library construction, this paper distinguishes between similar types of smart library and divides the concepts associated with smart library building into three dimensions: technology, service and human. Findings Traditional libraries can transform to smart libraries by strategic design and implementation of advanced technologies, such as cloud computing, data mining and artificial intelligence, but they also need to consider service building, user cultivation and librarian training. Originality/value Aligning to the three main dimensions of smart libraries (technology, service and human), this study clarifies the concept of the smart library and offers strategic principles: integration of infrastructures, construction of service and human learning. It provides guidelines and directions for public and academic libraries committed to becoming smart libraries.
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Li, Guixia, and Guiping Jiang. "Construction and Planning of Library Service Facilities System Based on Public Digital Culture Education in International Cultural Metropolis." Open House International 44, no. 3 (September 1, 2019): 64–67. http://dx.doi.org/10.1108/ohi-03-2019-b0017.

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To study the construction and planning of the library service facility system based on public digital culture teaching in international cultural metropolises, the main body of resources construction, channels, selection criteria, construction standards, user service policies, service methods and service objects of the National Digital Library of Russia were analyzed. The construction foundation, policy support and digital resource construction of the Chinese and Russian national digital libraries were compared. The results showed that the problem of digital copyright was solved while the library community was constructing digital resources according to the existing rules. Therefore, this method has certain implications for the construction of digital libraries in metropolitan areas in China.
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Kamen, Ruth. "Viewpoint." Art Libraries Journal 26, no. 2 (2001): 3–4. http://dx.doi.org/10.1017/s0307472200012104.

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Architecture, the focus of this issue, is held to be the ‘mother of the arts’. It is also referred to as the ‘queen of the decorative arts’. But in the family of art libraries and art librarianship, architecture is often a neglected stepchild. So I welcome the invitation extended in this issue of the Art Libraries Journal to the ‘Cinderella of the arts’, and hope that her appearance at the art ball will extend beyond midnight.Over the past quarter of a century or so that I have been an architectural librarian, I have sometimes wondered why architecture libraries are isolated from their sister libraries in the world of art and design, and why architecture libraries and librarians feel the need to separate themselves from their art and design siblings. Is it because the art community is uncomfortable with architecture? If so, is this because architecture is outside the mainstream of art education or art history teaching? Or is it because architecture libraries are generally part of professional institutes, schools of architecture or architectural firms, whose concerns and affiliations may have more in common with the engineering sciences (civil, structural, materials, environmental), the building and construction industries, urban and landscape design, town and country planning, and estate, project and business management, than with the more scholarly and historical focus of art libraries in museums and universities?
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Oloufa, Amr A., Masaaki Ikeda, and Tang-Hung Nguyen. "Resource-based simulation libraries for construction." Automation in Construction 7, no. 4 (May 1998): 315–26. http://dx.doi.org/10.1016/s0926-5805(98)00048-x.

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Dani, Maria. "PEPTIDE DISPLAY LIBRARIES: DESIGN AND CONSTRUCTION*." Journal of Receptors and Signal Transduction 21, no. 4 (January 2001): 469–88. http://dx.doi.org/10.1081/rrs-100107927.

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TECHEN, NATASCHA, RENÉE S. ARIAS, NEIL C. GLYNN, ZHIQIANG PAN, IKHLAS A. KHAN, and BRIAN E. SCHEFFLER. "Optimized construction of microsatellite‐enriched libraries." Molecular Ecology Resources 10, no. 3 (April 6, 2010): 508–15. http://dx.doi.org/10.1111/j.1755-0998.2009.02802.x.

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Dissertations / Theses on the topic "CONSTRUCTION OF LIBRARIES"

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Yaish, Sami Abdul-Rahman. "Construction and screening of plant genomic libraries." Thesis, Durham University, 1990. http://etheses.dur.ac.uk/6054/.

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A library of pea (Pisum sativum L) genomic DNA in bacteriophage EMBL3 was screened for seed storage protein genes of the legumin and vicilin families. Three genomic clones were isolated. One of the clones was found to contain a gene in the Leg A sub-family which was designated Leg E. The nucleotide and predicted amino acid sequence of Leg E were compared to those of Leg A. The coding sequences of both genes are strongly homologous with only 9 bases difference out of 1560 bases. A second genomic clone contained two genes from the Leg J subfamily. Leg J and Leg K. The clone was shown to overlap with a genomic clone isolated previously, JC5 (Gatehouse et al. 1988). Strong homology was found between the Leg K and Leg J sequences. The Leg K gene is predicted to be pseudogene, due to the conversion of the ATG methionine start codon to a GTG valine codon and the presence of a stop codon in the 5' end of the coding sequence in the reading frame predicted by the first subsequent start codon. A genomic library was constructed for Arabidopsis thaliana, using EMBL3 as a vector to sub-clone Sau3AI partially digested Arabidopsis genomic DNA. About 8 x 10(^4) random clones were obtained when the ligated vector DNA and insert were in vitro packaged. The Arabidopsis gene library was screened for clones containing sequences encoding the cell wall protein extensin, using a rape (Brassica napus L extensin cDNA as a probe. Six clones were isolated, two of which were restriction mapped. One of them was partially sequenced. This clone did not contain an extensin gene homologous to the probe sequence, and only contained a short extensin-like sequence which was responsible for the observed hybridisation. The putative gene may represent another type of protein, since it was expressed in the root of Arabidopsis and Brassica napus L, as shown by "Northern" blots which were probed with labelled DNA from the clone.
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Bouloc, Nathalie Sylvie. "New methodologies for the construction of polyether libraries." Thesis, University of Southampton, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324807.

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Braff, Jennifer C. "Construction and phenotypic screening of mid-size insert marine microbial environmental genomic libraries." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/43722.

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Thesis (S.M.)--Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering; and the Woods Hole Oceanographic Institution), 2008.
Includes bibliographical references (leaves 52-56).
Functional screening of environmental genomic libraries permits the identification of clones expressing activities of interest without requiring prior knowledge of the genes responsible. In this study, protocols were optimized for the construction of mid-size DNA insert, inducible expression environmental genomic plasmid libraries for this purpose. A library with a mean insert size of 5.2 kilobases was constructed with environmental DNA isolated from surface ocean water collected at Hawaii Ocean Time-series station ALOHA in plasmid cloning vector pMCL200 under the inducible control of the PLAC promoter. To begin to evaluate the utility of such libraries for gene expression-based screens, this library was screened phenotypically for clones expressing genes that confer fluorescence or distinctive coloration on colonies of host Escherichia coli cells, and results were compared to those for a fosmid library constructed from the same marine microbial DNA sample. Ecologically relevant sequences were identified in both libraries, and each was observed to offer both advantages and disadvantages. Results of this study suggest that mid-size insert plasmid libraries under the control of inducible promoters can provide a useful and complementary approach for both functional screening and shotgun sequencing of environmental genomic libraries.
by Jennifer C. Braff.
S.M.
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Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ficus (Signoret) /." Link to the online version, 2008. http://hdl.handle.net/10019/1884.

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Elmacı, Zehra Seda Arslanoğlu Alper. "Generation of improved E.coli strains to be used in the construction of ligand libraries/." [s.l.]: [s.n.], 2005. http://library.iyte.edu.tr/tezler/master/biyoteknoloji/T000320.pdf.

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Thesis (Master)--İzmir Institute of Technology, İzmir, 2005.
Keywords: Phage display technology, bactriophage, homologous recombination, M13, fusion protein. Includes bibliographical references (leaves. 56).
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Mann, William R. "Glucose-6-phosphate dehydrogenase : construction of yeast DNA libraries and screening for its gene." Thesis, University of Aberdeen, 1988. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU010105.

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The aim of the project was to determine the sequence of the gene encoding glucose-6-phosphate dehydrogenase (G6PDH) in the industrially used, but genetically little explored yeast Candida utilis. In the first strategy, a C. utilis DNA library was constructed in the expression vector phage lambda gt11, and was screened immunologically with antiserum raised against C. utilis G6PDH. The second strategy involved construction of libraries in the plasmid vector pTZ18R and screening with a mixed oligonucleotide probe. Two methods were developed that allow DNA, of appropriate quality for the construction of DNA libraries, to be prepared from C. utilis cells. Both involve centrifugation of C. utilis lysates through caesium trifluoroacetate gradients. A rabbit antiserum was raised against a commercial preparation of C. utilis G6PDH. It reacted with Western blotted C. utilis G6PDH, and non-specific binding of the antiserum to Western blotted E. coli protein was reduced by treatment with an E. coli extract. Antiserum pretreated in this way was used for immunological screening. Sequence information was determined for the inserts of 7 phage identified during immunological screening. None of the insert sequences was considered to contain part of the C. utilis G6PDH coding sequence. One putative positive colony was identified during the screening of the pTZ18R libraries. Sequence determination showed the insert to contain a region complementary to 16 bases in one of the 17-base long probe oligonucleotides. This insert was considered not to contain part of the C. utilis G6PDH coding sequence. Time did not permit further work which, using the methods developed, should now permit determination of the C. utilis G6PDH gene sequence.
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Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ficus (Signoret)." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/4083.

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Thesis (MSc (Genetics))--Stellenbosch University, 2008.
The vine mealybug, Planococcus ficus (Signoret), is a severe pest of grapevine in many grape and wine producing countries around the world. It is renowned not only for the considerable damage it infers to grapevine of its own accord, but in particular for its role in transmitting deleterious viral diseases such as grapevine leafroll disease, Kober stem grooving, Shiraz disease and corky bark. Incidentally, it is an exceptionally tenacious antagonist of grapevine, being resistant to both chemical and biological control mechanisms. As a result, finding an effective strategy for P. ficus control has become a main priority of viticultural industries worldwide. Possible implementation of biotechnological approaches to pest management has resulted in a need for P. ficus genetic data - of which there are currently very little available. The transcribed genes of an organism can be captured in a cDNA library, and the sequences of the various transcripts can then be characterized. In this study altogether five cDNA libraries were constructed from the transcribed sequences of Planococcus ficus (Signoret). Instrumental to their construction was the identification of an RNA extraction protocol that provided large quantities of high quality RNA from mealybugs. The five cDNA libraries were the result of a set of modifications to the Creator™ SMART™ cDNA Library Construction Kit (used for Primary Library construction), and differed mainly with regards to range of insert sizes they contain. Whereas an abundance of short fragments were found in the Primary Library (42% of screened inserts 60.5 kb, and 20% >1 kb), the Fractionated Libraries contained inserts of specific size ranges that were more-or-less equally represented. The broadest size range was found in Fractionated Library 4, for which a uniform distribution over the range 0.25 kb - 4 kb was observed. Average insert sizes of Fractionated Libraries 1 to 4 were estimated at 0.25 kb, 0.5 kb, 1 kb and 2 kb respectively. These results demonstrated the importance of using a protocol designed to circumvent the bias towards incorporation of shorter transcripts in cDNA libraries. Although the libraries were not exhaustively analyzed, the outcome of a pilot investigation indicated that 41% of the submitted sequences had matches in the non-redundant database of the National Center for Biotechnology Information (NCBI, E-value 6 10-5), and that approximately 82% of these were of insect origin. Moreover, two potential targets for an RNAi-mediated approach to P. ficus pest control were identified. With one exception, these sequences seemed to be unique to arthropods. Future research needs to investigate the efficiency by which these sequences are able to constrain P. ficus proliferation, and their suitability for grapevine transformation.
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Ugursal, Ahmet. "The effects of human behavior and information-transmitting medium on the library building typology." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1318943.

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Technological advancements in the second half of the century have significantly affected academic library institution. Libraries are undergoing a transition period and constantly updating their system to adapt to those advancements. However, library building is slow to adapt to the changes. This study examines those changes in academic libraries and buildings based on two main variables. namely human beings. and the medium. through which the information is transmitted The invention of first. electronic, and then digital systems significantly changed these two variables. The resulting library building is shaped by the new human Behavior and the form of information-mediums in a technology-oriented environment. The result of this study is 'five governing principles,' which explain the position of those two variables in a complex web of relations created by technological advancements. The same principles also explain the role of different variables in the future of the library building typology.
Department of Architecture
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Montgomery, Shirley A. "A new profile for a familiar building : a carbon-neutral public library branch in Union Township in Anderson, Indiana." Virtual Press, 2008. http://liblink.bsu.edu/uhtbin/catkey/1390313.

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Buildings can be built that use no energy from external power grids. They can be essentially carbon neutral in their environmental load, and they can be built and operated at fair market values. Although this may sound like a dream of the future, the World Business Council for Sustainable Development (WBCSD) is working toward making that dream a reality today. The Council announced on March 26, 2006 (Environment News Service) that it is forming an alliance of global companies to ensure that by 2050 all new buildings meet these standards.1The profession of architecture, at the beginning of the 21st century, finds itself in a position where architectural philosophies and methodologies must respond to the world changing events that are impacting our planet. This creative project outlines some of the methodologies used to create a carbon-neutral public library. Within a public library, architects respond to two of the primary concerns of this young century; the global need for a clean, livable planet and the proliferation of digital media and methods that are driving globalization as we are beginning to know it.The proposed new Union Township Branch of the Anderson Public Library seemed a logical subject for this Creative Project because much of the past work of our firm and much of my personal experience involved the design of public libraries. The carbon neutral concepts evolved gradually, after much study, and the goal of this creative project is that this particular library and many other sustainable libraries in the future will benefit from the concepts and methodologies outlined here, as those new buildings approach carbon neutrality.
Department of Architecture
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Jaimes, Oscar O. "Construction and characterization of cDNA libraries bearing expressed sequence tags induced after treatment of Vicia faba leaves with saliylic acid." Berlin VWF, 2005. http://deposit.ddb.de/cgi-bin/dokserv?id=2709287&prov=M&dok_var=1&dok_ext=htm.

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Books on the topic "CONSTRUCTION OF LIBRARIES"

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New York (State). Legislature. Assembly. Standing Committee on Libraries and Education Technology. Statewide library construction. Albany, N.Y: Associated Reporters International, Inc., 2005.

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Sutton, Sally. Construction. Newtown, N.S.W: Walker Books Australia, 2014.

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Hargrave, Roger. Office library systems: A guide for the construction industry. London: Architectural Press, 1987.

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Léon, Laborde. Etude sur la construction des bibliothèques. Hildesheim: Olms, 1993.

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Fu, Michael C. The SEDAR reuse libraries. [Champaign, IL]: US Army Corps of Engineers, Construction Engineering Research Laboratories, 1997.

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United States. Office of Educational Research and Improvement. Library Programs., ed. Public library construction: An overview and analysis, fiscal year 1987. Washington, D.C: Office of Educational Research and Improvement, U.S. Dept. of Education, 1990.

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Libraries. London [England]: Architectural Press, 1986.

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Anastasakis, Manolis. Redesigning libraries for schools: A proposal for secondary education. Athens: Metaichimo Publications, 2003.

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Library Association. Branch and Mobile Libraries Group., ed. Mobile libraries in England and Wales: A guide to their construction and use. 2nd ed. St. Albans: Branch and Mobile Libraries Group of the Library Association, 1985.

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Bennett, Scott. Libraries designed for learning. Washington, D.C: Council on Library and Information Resources, 2003.

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Book chapters on the topic "CONSTRUCTION OF LIBRARIES"

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Caucheteur, Déborah, Gautier Robin, Vincent Parez, and Pierre Martineau. "Construction of Synthetic Antibody Libraries." In Antibody Engineering, 93–108. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8648-4_5.

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Fehrsen, Jeanni, Susan Wemmer, and Wouter van Wyngaardt. "Construction of Chicken Antibody Libraries." In Methods in Molecular Biology, 189–203. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7447-4_10.

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Avril, Arnaud, Sebastian Miethe, Michael Hust, and Thibaut Pelat. "Construction of Macaque Immune-Libraries." In Methods in Molecular Biology, 83–112. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7447-4_5.

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Nguyen, Thi Thu Ha, Jong Seo Lee, and Hyunbo Shim. "Construction of Rabbit Immune Antibody Libraries." In Methods in Molecular Biology, 133–46. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7447-4_7.

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Quail, Mike A., Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter. "Genomic Libraries: I. Construction and Screening of Fosmid Genomic Libraries." In Methods in Molecular Biology, 37–58. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-228-1_3.

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Carstens, Carsten P., Katherine A. Felts, and Sarah E. Johns. "Construction of CRISPR Libraries for Functional Screening." In Synthetic Biology, 139–50. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7795-6_7.

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Pasello, Michela, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, and Katia Scotlandi. "Construction of Human Naïve Antibody Gene Libraries." In Antibody Engineering, 73–91. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8648-4_4.

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Nilvebrant, Johan, and Sachdev S. Sidhu. "Construction of Synthetic Antibody Phage-Display Libraries." In Methods in Molecular Biology, 45–60. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7447-4_3.

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Hust, Michael, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel. "Construction of Human Naive Antibody Gene Libraries." In Antibody Engineering, 85–107. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-974-7_5.

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Martin, Gregory B. "Construction of plant yeast artificial chromosome libraries." In Plant Molecular Biology Manual, 383–99. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0511-8_25.

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Conference papers on the topic "CONSTRUCTION OF LIBRARIES"

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Reif, Michael, Michael Eichberg, Ben Hermann, Johannes Lerch, and Mira Mezini. "Call graph construction for Java libraries." In FSE'16: 24nd ACM SIGSOFT International Symposium on the Foundations of Software Engineering. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/2950290.2950312.

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Linghui Guo. "On construction of digital libraries in universities." In 2010 3rd IEEE International Conference on Computer Science and Information Technology (ICCSIT 2010). IEEE, 2010. http://dx.doi.org/10.1109/iccsit.2010.5564750.

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Yeh, Lo-Yao, Peggy Joy Lu, and Jen-Wei Hu. "NCHC Blockchain Construction Platform (NBCP): Rapidly Constructing Blockchain Nodes around Taiwan." In 2017 ACM/IEEE Joint Conference on Digital Libraries (JCDL). IEEE, 2017. http://dx.doi.org/10.1109/jcdl.2017.7991607.

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Xinfang Yuan. "Discussion on the cultural construction of digital libraries." In 2010 3rd International Conference on Advanced Computer Theory and Engineering (ICACTE 2010). IEEE, 2010. http://dx.doi.org/10.1109/icacte.2010.5579525.

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Almaimani, A. M., and and N. O. Nawari. "BIM-Driven Islamic Construction: Part 2—Digital Libraries." In 2015 International Workshop on Computing in Civil Engineering. Reston, VA: American Society of Civil Engineers, 2015. http://dx.doi.org/10.1061/9780784479247.057.

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Wang, XinYu, and QingSong Zhang. "A Study of Key Subject Construction Oriented Document Resource Construction of University Libraries." In International Conference on Education, Management and Information Technology. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/icemit-15.2015.153.

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"Role of University Libraries+ in the Campus Culture Construction." In 2020 International Conference on Social Sciences and Social Phenomena. Scholar Publishing Group, 2020. http://dx.doi.org/10.38007/proceedings.0001100.

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Linghui Guo. "On construction of knowledge management in university digital libraries." In 2010 3rd IEEE International Conference on Computer Science and Information Technology (ICCSIT 2010). IEEE, 2010. http://dx.doi.org/10.1109/iccsit.2010.5564968.

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Li, Hao-qing, and Le-peng Wang. "Organizational Culture Construction Based on Knowledge Sharing at Libraries." In 2011 International Conference on Management and Service Science (MASS 2011). IEEE, 2011. http://dx.doi.org/10.1109/icmss.2011.5998959.

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Lv, Jinyu. "Research and Practice on the Construction of Hackerspace in Libraries." In 2nd International Conference on Electronics, Network and Computer Engineering (ICENCE 2016). Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/icence-16.2016.186.

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Reports on the topic "CONSTRUCTION OF LIBRARIES"

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Phung, Wilson, Christopher Hack, Harris Shapiro, Susan Lucas, and Jan-Fang Cheng. Scaling up the 454 Titanium Library Construction and Pooling of Barcoded Libraries. Office of Scientific and Technical Information (OSTI), March 2009. http://dx.doi.org/10.2172/1012471.

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Deshpande, Shweta, Christopher Hack, Eric Tang, Stephanie Malfatti, Aren Ewing, Susan Lucas, and Jan-Fang Cheng. Development of High Throughput Process for Constructing 454 Titanium and Illumina Libraries. Office of Scientific and Technical Information (OSTI), May 2010. http://dx.doi.org/10.2172/985370.

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Peng, Ze, Ze Peng, Matthew Hamilton, Sara Ting, Hank Tu, Eugene Goltsman, Alla Lapidus, Susan Lucas, and Jan-Fang Cheng. Modification of the GS LT Paired-end Library Protocol for Constructing Longer Insert Size Libraries. Office of Scientific and Technical Information (OSTI), May 2008. http://dx.doi.org/10.2172/935407.

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Nelson, Nathan, and Randy Schekman. Functional Biogenesis of V-ATPase in the Vacuolar System of Plants and Fungi. United States Department of Agriculture, September 1996. http://dx.doi.org/10.32747/1996.7574342.bard.

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Abstract:
The vacuolar H+-ATPase (V-ATPase) is one of the most fundamental enzymes in nature. It pumps protons into the vacuolar system of eukaryotic cells and provides the energy for numerous transport systems. Through our BARD grant we discovered a novel family of membrane chaperones that modulate the amount of membrane proteins. We also elucidated the mechanism by which assembly factors guide the membrane sector of V-ATPase from the endoplasmic reticulum to the Golgi apparatus. The major goal of the research was to understand the mechanism of action and biogenesis of V-ATPase in higher plants and fungi. The fundamental question of the extent of acidification in organelles of the vacuolar system was addressed by studying the V-ATPase of lemon fruit, constructing lemon cDNAs libraries and study their expression in mutant yeast cells. The biogenesis of the enzyme and its function in the Golgi apparatus was studied in yeast utilizing a gallery of secretory mutants available in our laboratories. One of the goals of this project is to determine biochemically and genetically how V-ATPase is assembled into the different membranes of a wide variety of organelles and what is the mechanism of its action.The results of this project advanced out knowledge along these lines.
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5

Bercovier, Herve, Raul Barletta, and Shlomo Sela. Characterization and Immunogenicity of Mycobacterium paratuberculosis Secreted and Cellular Proteins. United States Department of Agriculture, January 1996. http://dx.doi.org/10.32747/1996.7573078.bard.

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Abstract:
Our long-term goal is to develop an efficient acellular vaccine against paratuberculosis based on protein antigen(s). A prerequisite to achieve this goal is to analyze and characterize Mycobacterium paratuberculosis (Mpt) secreted and cellular proteins eliciting a protective immune response. In the context of this general objective, we proposed to identify, clone, produce, and characterize: the Mpt 85B antigen and other Mpt immunoreactive secreted proteins, the Mpt L7/L12 ribosomal protein and other immunoreactive cellular proteins, Mpt protein determinants involved in invasion of epithelial cells, and Mpt protein antigens specifically expressed in macrophages. Paratuberculosis is still a very serious problem in Israel and in the USA. In the USA, a recent survey evaluated that 21.6% of the dairy herd were infected with Mpt resulting in 200-250 million dollars in annual losses. Very little is known on the virulence factors and on protective antigens of Mpt. At present, the only means of controlling this disease are culling or vaccination. The current vaccines do not allow a clear differentiation between infected and vaccinated animals. Our long-term goal is to develop an efficient acellular paratuberculosis vaccine based on Mpt protein antigen(s) compatible with diagnostic tests. To achieve this goal it is necessary to analyze and characterize secreted and cellular proteins candidate for such a vaccine. Representative Mpt libraries (shuttle plasmid and phage) were constructed and used to study Mpt genes and gene products described below and will be made available to other research groups. In addition, two approaches were performed which did not yield the expected results. Mav or Mpt DNA genes that confer upon Msg or E. coli the ability to invade and/or survive within HEp-2 cells were not identified. Likewise, we were unable to characterize the 34-39 kDa induced secreted proteins induced by stress factors due to technical difficulties inherent to the complexity of the media needed to support substantial M. pt growth. We identified, isolated, sequenced five Mpt proteins and expressed four of them as recombinant proteins that allowed the study of their immunological properties in sensitized mice. The AphC protein, found to be up regulated by low iron environment, and the SOD protein are both involved in protecting mycobacteria against damage and killing by reactive oxygen (Sod) and nitrogen (AhpC) intermediates, the main bactericidal mechanisms of phagocytic cells. SOD and L7/L12 ribosomal proteins are structural proteins constitutively expressed. 85B and CFP20 are both secreted proteins. SOD, L7/L12, 85B and CFP20 were shown to induce a Th1 response in immunized mice whereas AphC was shown by others to have a similar activity. These proteins did not interfere with the DTH reaction of naturally infected cows. Cellular immunity provides protection in mycobacterial infections, therefore molecules inducing cellular immunity and preferentially a Th1 pathway will be the best candidate for the development of an acellular vaccine. The proteins characterized in this grant that induce a cell-mediated immunity and seem compatible with diagnostic tests, are good candidates for the construction of a future acellular vaccine.
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