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Journal articles on the topic 'Contaminant Detection and Isolation'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Alexis, Kyriacou, Michaelides Michalis, Reppa Vasso, Timotheou Stelios, G. Panayiotou Christos, and M. Polycarpou Marios. "Distributed Contaminant Detection and Isolation for Intelligent Buildings." IEEE Transactions on Control Systems Technology 26, no. 6 (2017): 1925–41. https://doi.org/10.5281/zenodo.1012296.

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The automatic preservation of the Indoor Air Quality is an important task of the intelligent building design in order to ensure the health and safety of the occupants. The indoor air quality, however, is often compromised by various airborne contaminants that penetrate the indoor environment as a result of accidents or planned attacks. In this work, we provide the detailed analysis, implementation and evaluation of a distributed methodology for detecting and isolating multiple contaminant events in large-scale buildings. Specifically, we consider the&nbs

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Kyriacou, Alexis, Michalis P. Michaelides, Vasso Reppa, Stelios Timotheou, Christos G. Panayiotou, and Marios M. Polycarpou. "Distributed Contaminant Detection and Isolation for Intelligent Buildings." IEEE Transactions on Control Systems Technology 26, no. 6 (2018): 1925–41. http://dx.doi.org/10.1109/tcst.2017.2754986.

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3

Baranowski, T. M., and E. J. LeBoeuf. "Consequence Management Optimization for Contaminant Detection and Isolation." Journal of Water Resources Planning and Management 132, no. 4 (2006): 274–82. http://dx.doi.org/10.1061/(asce)0733-9496(2006)132:4(274).

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4

Alexis, Kyriacou, P. Michaelides Michalis, G. Eliades Demetrios, G. Panayiotou Christos, and M. Polycarpou Marios. "COMOB: A MATLAB toolbox for sensor placement and contaminant event monitoring in multi-zone buildings." Building and Environment Volume 154 (May 1, 2019): 348–61. https://doi.org/10.1016/j.buildenv.2019.02.013.

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The monitoring of the indoor air quality against the presence of contaminant sources is of paramount importance since it directly influences the occupants’ comfort, productivity, health and safety. Various approaches exist in the literature for monitoring and controlling the building environment. However, there is an identified lack of automated tools for evaluating, testing and comparing such approaches. This paper presents COMOB (COntaminat MOnitoring in Buildings), a developed MATLAB toolbox for sensor placement and contaminant monitoring in buildings. Specifically, the COMOB toolbox

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Alexis, Kyriacou, Timotheou Stelios, Michaelides Michalis, Panayiotou Christos, and Polycarpou Marios. "Partitioning of Intelligent Buildings for Distributed Contaminant Detection and Isolation." IEEE Transactions on Emerging Topics in Computational Intelligence 1, no. 2 (2017): 72–86. https://doi.org/10.5281/zenodo.1012286.

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Intelligent buildings are responsible for ensuring indoor air quality for their occupants under normal operation as well as under possibly harmful contaminant events. An emerging environmental application involves the monitoring of intelligent buildings against harmful events by incorporating various sensing technologies and using sophisticated algorithms to detect and isolate such events. In this context, both centralized and distributed approaches have been proposed, with the latter having significant benefits in terms of complexity, scalability, reliabilit

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Kyriacou, Alexis, Stelios Timotheou, Michalis P. Michaelides, Christos Panayiotou, and Marios Polycarpou. "Partitioning of Intelligent Buildings for Distributed Contaminant Detection and Isolation." IEEE Transactions on Emerging Topics in Computational Intelligence 1, no. 2 (2017): 72–86. http://dx.doi.org/10.1109/tetci.2017.2665119.

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Blumenthal, M. M., and J. R. Stockler. "Isolation and detection of alkaline contaminant materials (ACM) in used frying oils." Journal of the American Oil Chemists' Society 63, no. 5 (1986): 687–88. http://dx.doi.org/10.1007/bf02638238.

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Eman A.R. Al-Mazorey and Saleh Easa Mohammad. "Isolation and Identification of Some contaminant fungi and Detection of Aflatoxin in flour and produced bread and loaf in Duhok city." Tikrit Journal of Pure Science 21, no. 7 (2023): 7–14. http://dx.doi.org/10.25130/tjps.v21i7.1103.

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This study aimed to isolate and detect some contaminant fungi and its produced mycotoxins on flour and its produced bread and loaf in Duhok city, Kurdistan Region, Iraq, This study included samples of local and imported flour (White & Black), and also the produced bread and loaf. The study showed different species of contaminant fungi , and the fungus Aspergillus flavus was the dominant fungus, which isolated from both the local black flour and Iranian black flour, and fungus Penicillium sp, which isolated from both the local white flour and Iranian black flour, then fungus A.niger,

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Creger, Richard J., Kisa E. Weeman, Michael R. Jacobs, et al. "Lack of Utility of the Lysis-Centrifugation Blood Culture Method for Detection of Fungemia in Immunocompromised Cancer Patients." Journal of Clinical Microbiology 36, no. 1 (1998): 290–93. http://dx.doi.org/10.1128/jcm.36.1.290-293.1998.

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We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and clinical criteria. Fifty-three patients had 66 positive blood cultures for fungi, nine on multiple occasions. In 20 episodes true pathogens were recovered, 6 from broth medium alone, 4 from the Isolator system alone, and 10 from both systems. False-negative cultures were noted in 4 o

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10

Hanson, Patricia, Nicole Mitchell, S. Brian Caudle, et al. "A Study to Evaluate the Need for Comminution in the Preparation of Food Materials for Testing of Listeria monocytogenes, Staphylococcus Species, and Generic Escherichia coli." Journal of AOAC INTERNATIONAL 103, no. 6 (2020): 1639–45. http://dx.doi.org/10.1093/jaoacint/qsaa074.

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Abstract Background Comminution reduces the sampling error arising from distributional heterogeneity of the target contaminant/target analyte in the material, facilitating the selection of a more representative test portion. A laboratory sampling method incorporating comminution prior to selection of the test portion (Sampling Method B) was compared to current sampling methods that used no comminution step (Sampling Method A). Objective This required the development of an efficient process for comminution of food samples prior to removal of the test portion for the detection and isolation of L

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11

Westmeijer, George, Cristina Escudero, Claudia Bergin, et al. "Continental scientific drilling and microbiology: (extremely) low biomass in bedrock of central Sweden." Biogeosciences 21, no. 2 (2024): 591–604. http://dx.doi.org/10.5194/bg-21-591-2024.

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Abstract. Scientific drilling expeditions offer a unique opportunity to characterize microbial communities in the subsurface that have long been isolated from the surface. With subsurface microbial biomass being low in general, biological contamination from the drilling fluid, sample processing, or molecular work is a major concern. To address this, characterization of the contaminant populations in the drilling fluid and negative extraction controls are essential for assessing and evaluating such sequencing data. Here, rock cores down to 2250 m depth, groundwater-bearing fractures, and the dr

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12

Ibrahim, Hytham H., Manar F. Seioudy, Shaimaa A. Mohamed, and Magda M. Sayed. "Detection and characterization of endogenous avian leukosis virus obtained from contaminated avian vaccines." Global Animal Science Journal 8, no. 1 (2020): 17–31. https://doi.org/10.5281/zenodo.3663601.

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In this study the reverse transcriptase polymerase chain reaction (RT- PCR) and the enzyme linked immunosorbent assay (ELISA) were utilized for screening of different live avian viral vaccines to detect biocontamination with avian leukosis virus (ALV). By using ELISA test, the group specific antigen of avian leukosis virus was detected in three batches of Marek’s disease virus (MDV) vaccines as well as in one batch of avian encephalomyelitis virus (AEV) vaccine. To identify the subgroup of the contaminant ALV, contaminated samples were tested by RT – PCR using sets of primers speci

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Hammadi, Raad Jabbar. "Detection of E. coli, as a contaminant of minced meat in certain locations of Baqubah city-Diyala Province-Iraq." Kufa Journal For Veterinary Medical Sciences 8, no. 1 (2017): 72–78. http://dx.doi.org/10.36326/kjvs/2017/v8i14312.

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This survey investigated the prevalence of Escherichia coli (E.coli)in raw minced beef meat collected from different areas of Baqubah city in Diyala Province for detection of contamination with E.coli. A(90) samples were collected at (10)samples from each area in Baqubah city.and different dilutions were made for each one (10-1 , 10-2 , 10-3 ,10-4 ).These sample were pour onto MacConkey Agar and thenstreak onto eosin methylene blue agar(EMB) plates.The results was shown presence of high contamination ratio of the microbial isolation from minced beef meat of E.coli bacteria in different areas o

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Hammadi, Raad Jabbar. "Detection of E. coli, as a contaminant of minced meat in certain locations of Baqubah city-Diyala Province-Iraq." Kufa Journal For Veterinary Medical Sciences 8, no. 1 (2017): 72–78. http://dx.doi.org/10.36326/kjvs/2017/v8i14312.

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This survey investigated the prevalence of Escherichia coli (E.coli)in raw minced beef meat collected from different areas of Baqubah city in Diyala Province for detection of contamination with E.coli. A(90) samples were collected at (10)samples from each area in Baqubah city.and different dilutions were made for each one (10-1 , 10-2 , 10-3 ,10-4 ).These sample were pour onto MacConkey Agar and thenstreak onto eosin methylene blue agar(EMB) plates.The results was shown presence of high contamination ratio of the microbial isolation from minced beef meat of E.coli bacteria in different areas o

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15

Kalinina, O. S. "Viruses in food products." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 23, no. 103 (2021): 15–20. http://dx.doi.org/10.32718/nvlvet10303.

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Data on viral food contaminants that are actually or potentially capable of realizing the food route of infection are presented. The main sources of infection of food with viruses are named: human waste / faeces, contaminated food processing facilities, animals-carriers of zooanthroponotic infections. The groups of viruses transmitted through food are characterized: 1) gastroenteritis pathogens – Sapporo and Norwalk viruses from the family Caliciviridae; Rotavirus A from the family Reoviridae; Mammastroviruses 1, 6, 8 and 9 from the family Astroviridae; Human mastadenovirus F from the family A

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Jimenez, Luis, and Stacey Smalls. "Molecular Detection of Burkholderia cepacia in Toiletry, Cosmetic, and Pharmaceutical Raw Materials and Finished Products." Journal of AOAC INTERNATIONAL 83, no. 4 (2000): 963–66. http://dx.doi.org/10.1093/jaoac/83.4.963.

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Abstract A polymerase chain reaction (PCR) assay was developed and compared with standard methods for rapid detection of Burkholderia cepacia, a major industrial contaminant, in cosmetic and pharmaceutical raw materials and finished products. Artificially contaminated samples were incubated for 24 h in trypticase soy broth containing 4% Tween 20 and 0.5% soy lecithin. DNA was extracted from each sample using a proteinase K-tris-EDTA-Tween 20 treatment at 35°C. The extracted DNA was added to Ready-To-Go PCR beads and specific DNA primers for B. cepacia. The B. cepacia DNA primers coded for a 20

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Afshar, Abbas, and Ehsan Najafi. "Consequence management of chemical intrusion in water distribution networks under inexact scenarios." Journal of Hydroinformatics 16, no. 1 (2013): 178–88. http://dx.doi.org/10.2166/hydro.2013.125.

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The US Environmental Protection Agency (EPA)'s Response Protocol Toolbox provides a list of recommendations on actions that may be taken to minimize the potential threats to public health following a contamination threat. This protocol comprises three steps: (1) detection of contaminant presence, (2) source identification and (3) consequence management. This paper intends to explore consequence management under source uncertainty, applying Minimize Maximum Regret (MMR) and Minimize Total Regret (MTR) approaches. An ant colony optimization algorithm is coupled with the EPANET network solver for

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18

Noble, Cora A., and Steven Stevenson. "Detection and Isolation of Isomerically Pure C120 Fullertubes." ECS Meeting Abstracts MA2022-01, no. 11 (2022): 834. http://dx.doi.org/10.1149/ma2022-0111834mtgabs.

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The emergence of macroscopic amounts of C60 fullerenes and carbon nanotubes in the 1990s has led to an explosion of growth in new structures of carbon nanomaterials. Over the last decades, there have been theoretical predictions of a particular “hybrid” family of mathematical structures. Fullertubes represent a new area of fundamental research in molecular carbon. They are similar to fullerenes in structure, but with increasingly long cylindrical, single-wall nanotube belts in the middle of the molecules. These nanotube belts are similar to single-layer, rolled graphene. In 2020 we published t

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19

Huang, Kun, Sudha Garimella, Alyssa Clay-Gilmour, et al. "Comparison of Human Urinary Exosomes Isolated via Ultracentrifugation Alone versus Ultracentrifugation Followed by SEC Column-Purification." Journal of Personalized Medicine 12, no. 3 (2022): 340. http://dx.doi.org/10.3390/jpm12030340.

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Chronic kidney disease is a progressive, incurable condition that involves a gradual loss of kidney function. While there are no non-invasive biomarkers available to determine whether individuals are susceptible to developing chronic kidney disease, small RNAs within urinary exosomes have recently emerged as a potential candidate to use for assessing renal function. Ultracentrifugation is the gold standard for urinary exosome isolation. However, extravesicular small RNA contamination can occur when isolating exosomes from biological fluids using ultracentrifugation, which may lead to misidenti

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20

Rusiňáková, Katarína, Michal Kirchner, and Svetlana Hrouzková. "Analytical Methods for the Detection of Contaminants in Crustaceans and Mollusks." Chemické listy 116, no. 8 (2022): 481–93. http://dx.doi.org/10.54779/chl20220481.

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The paper overviews analytical methods for the detection of a variety of contaminants, such as pesticide residues, polyaromatic hydrocarbons, polychlorinated biphenyls, etc. in environmental samples – crustaceans and mollusks. Complexity of analyzed samples requires a sophisticated sample preparation: mostly two-step procedures include an extraction followed by a clean-up of the extract. QuEChERES technique complies with the requirements of the green analytical chemistry and is preferred for the isolation of pesticide residues. Polyaromatic hydrocarbons are extracted from samples utilizing Sox

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DERZELLE, SYLVIANE, FRANÇOISE DILASSER, VÉRONIQUE MALADEN, et al. "Comparison of Three Chromogenic Media and Evaluation of Two Molecular-Based Identification Systems for the Detection of Enterobacter sakazakii from Environmental Samples from Infant Formulae Factories." Journal of Food Protection 70, no. 7 (2007): 1678–84. http://dx.doi.org/10.4315/0362-028x-70.7.1678.

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Enterobacter sakazakii is an occasional contaminant of powdered infant formula that can cause rare but severe foodborne infections in infants. To determine optimal methods for the detection and identification of E. sakazakii, 38 naturally contaminated samples from infant formulae factories were analyzed by two PCR-based methods and by a method (TS 22964/RM 210) developed by the International Organization for Standardization and the International Dairy Federation (ISO-IDF) using three different commercial chromogenic agars. The ISO-IDF method includes two enrichment steps, plating of the second

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Xu, Guohao, Gaozhe Cai, Lijuan Liang, et al. "Recent Advances in Microfluidics-Based Monitoring of Waterborne Pathogens: From Isolation to Detection." Micromachines 16, no. 4 (2025): 462. https://doi.org/10.3390/mi16040462.

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Waterborne pathogens seriously threaten human life and can cause diarrhea, gastrointestinal disorders, and more serious systemic infections. These pathogens are usually caused by contaminated water sources that contain disease-causing microorganisms, such as bacteria, viruses, and parasites, which cause infection and disease when they enter the human body through drinking water or other means. Due to the wide range of transmission routes and the high potential risk of waterborne pathogens, there is an urgent need for an ultrasensitive, rapid, and specific pathogenic microorganism monitoring pl

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23

Loor-Giler, Anthony, Marcela Robayo-Chico, Byron Puga-Torres, et al. "Escherichia coli O157:H7, a Common Contaminant of Raw Milk from Ecuador: Isolation and Molecular Identification." Foods 14, no. 3 (2025): 410. https://doi.org/10.3390/foods14030410.

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Escherichia coli (E. coli), especially the Shiga toxin-producing O157:H7 strain, poses severe health risks. In rural Ecuador, raw milk consumption heightens contamination risks. This study analyzed 633 raw milk samples from Pichincha and Manabí to assess E. coli O157:H7 prevalence. The samples were enriched using BHI broth, and then specific culture media were used to isolate E. coli O157:H7. The pathogen in the enriched raw milk was identified, and the isolates were specifically confirmed through the application of a newly designed qPCR assay. The novel qPCR assay demonstrated remarkable sens

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Liu, Yuan, Jian Wang, Fengli Wang, et al. "Preparation of Molecularly Imprinted Polymer for Selective Solid-Phase Extraction and Simultaneous Determination of Five Sulfonylurea Herbicides in Cereals." Sains Malaysiana 51, no. 6 (2022): 1707–24. http://dx.doi.org/10.17576/jsm-2022-5106-09.

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Molecular imprinting polymer (MIP) has been increasingly employed for sulfonylurea herbicides (SUHs) detection in different matrices. A novel MIP that was effective as a highly class-selective sorbent in molecularly imprinted solid-phase extraction (MISPE) was successfully prepared for isolation and purification of SUHs, namely, metsulfuron-methyl, chlorsulfuron, chlorimuron-ethyl, prosulfuron, and pyrazosulfuron-ethyl, in rice, corn and soybean samples. The MIP was synthesized by precipitation polymerization using metsulfuron-methyl as the template, 4-vinylpyridine as the functional monomer,

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Xu, Wenyan, and Linlin Zhao. "An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA–Protein Cross-Links from Mammalian Cells." DNA 2, no. 4 (2022): 264–78. http://dx.doi.org/10.3390/dna2040019.

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DNA–Protein cross-links (DPCs) are cytotoxic DNA lesions with a protein covalently bound to the DNA. Although much has been learned about the formation, repair, and biological consequences of DPCs in the nucleus, little is known regarding mitochondrial DPCs. This is due in part to the lack of robust and specific methods to measure mitochondrial DPCs. Herein, we reported an enzyme-linked immunosorbent assay (ELISA)-based method for detecting mitochondrial DPCs formed between DNA and mitochondrial transcription factor A (TFAM) in cultured human cells. To optimize the purification and detection w

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26

Hanifa, Muril Hizriyana, Wiwiek Tyasningsih, and Maya Nurwantanti Yunita. "Detection of Contamination Salmonella sp. of Beef in Banyuwangi Traditional Market." Jurnal Medik Veteriner 5, no. 2 (2022): 247–51. http://dx.doi.org/10.20473/jmv.vol5.iss2.2022.247-251.

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Salmonella sp. is one of the bacteria from Food-Borne Disease agents, can contaminate meat and cause salmonellosis. According to National Indonesian Standard 2009, meat is safe for consumption if it has a negative result of salmonella in 25 grams of meat. The purpose of this study was to determine whether there was contamination of Salmonella sp. on beef sold in the traditional market of Banyuwangi District. A total of 18 samples used in this study were taken according to the criteria for isolation and identification. All samples were enriched with the pre-enrichment stage using Lactose Broth

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Mahmood, Majid Mohammed, Moutaz Abdul Wahid Abdul-AL-Mounam, and Basil Razook Faraj. "Detection of Bacterial and Fungal Contaminants of the Imported Semen Straws Used for Cows." Kufa Journal For Veterinary Medical Sciences 4, no. 1 (2013): 23–30. http://dx.doi.org/10.36326/kjvs/2013/v4i13921.

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The study was conducted to detect the presence of bacterial and fungal contamination in imported semen straws used for Artificial Insemination (AI) in cows and antibiotic sensitivity test for those isolates. For this purpose, a total of 118 imported semen straws from local markets in Baghdad and some veterinarian clinics from December 2011 to February 2012 transported directly to the laboratories of Unit of Zoonotic Diseases (College of Veterinary Medicine / Baghdad University) frozen in liquid nitrogen (-196 ºC), the cultured on brain and heart broth and Sabouraud dextrose broth with routine

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Mahmood, Majid Mohammed, Moutaz Abdul Wahid Abdul-AL-Mounam, and Basil Razook Faraj. "Detection of Bacterial and Fungal Contaminants of the Imported Semen Straws Used for Cows." Kufa Journal For Veterinary Medical Sciences 4, no. 1 (2013): 23–30. http://dx.doi.org/10.36326/kjvs/2013/v4i13921.

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The study was conducted to detect the presence of bacterial and fungal contamination in imported semen straws used for Artificial Insemination (AI) in cows and antibiotic sensitivity test for those isolates. For this purpose, a total of 118 imported semen straws from local markets in Baghdad and some veterinarian clinics from December 2011 to February 2012 transported directly to the laboratories of Unit of Zoonotic Diseases (College of Veterinary Medicine / Baghdad University) frozen in liquid nitrogen (-196 ºC), the cultured on brain and heart broth and Sabouraud dextrose broth with routine

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29

HARA-KUDO, Y., Y. KASUGA, A. KIUCHI, T. HORISAKA, T. KAWASUMI, and S. KUMAGAI. "Increased Sensitivity in PCR Detection of tdh-Positive Vibrio parahaemolyticus in Seafood with Purified Template DNA." Journal of Food Protection 66, no. 9 (2003): 1675–80. http://dx.doi.org/10.4315/0362-028x-66.9.1675.

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PCR is an important method for the detection of thermostable direct hemolysin gene (tdh)–positive (pathogenichemolysin-producing) strains of Vibrio parahaemolyticus in seafood because tdh-negative (nonpathogenic) V. parahaemolyticus strains often contaminate seafood and interfere with the direct isolation of tdh-positive V. parahaemolyticus. In this study, the use of PCR to detect the tdh gene of V. parahaemolyticus in various seafoods artificially contaminated with tdh-positive V. parahaemolyticus was examined. PCR was inhibited by substances in oysters, squid, mackerel, and yellowtail but no

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Gorny, Adrienne M., Frank S. Hay, Xiaohong Wang, and Sarah J. Pethybridge. "Isolation of nematode DNA from 100 g of soil using Fe3O4 super paramagnetic nanoparticles." Nematology 20, no. 3 (2018): 271–83. http://dx.doi.org/10.1163/15685411-00003140.

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An economical method for extracting nematode DNA from 100 g of soil was developed to facilitate nematode detection and quantification, and tested using the Northern root-knot nematode,Meloidogyne hapla. The method utilised enzymatic laundry detergent lysis, Fe3O4super paramagnetic iron oxide nanoparticle (SPION) capture, and polyvinylpolypyrrolidone (PVPP) purification. Resultant DNA from this SPION capture method was approximately 100-fold less but of similar quality to DNA obtained from a standard phenol procedure and a commercial DNA extraction kit. An addition of 10 mg of nanoparticles to

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Hsieh, Yi-Cheng, Kyung-Min Lee, Toni Poole, Mick Runyon, Ben Jones, and Timothy J. Herrman. "Detection and Isolation of Salmonella spp. in Animal Feeds from 2007-2011." Journal of Regulatory Science 2, no. 1 (2014): 14–27. http://dx.doi.org/10.21423/jrs.regsci.2113.

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Salmonella species (spp.) are zoonotic pathogens that contaminate animal ingredients and finished feed and represent a significant hazard as identified by the Codex Animal Feed Taskforce. The United States (US) Food and Drug Administration (FDA) promulgated regulations prohibiting Salmonella contamination in feed and has since published a draft guidance document expressing their current strategy involving regulatory oversight of Salmonella contamination in feed. The Office of the Texas State Chemist (OTSC) initiated the broad surveillance of Salmonella spp. in 2007 in response of a Salmonella

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SATOKARI, REETTA, RIIKKA JUVONEN, ATTE VON WRIGHT, and AULI HAIKARA. "Detection of Pectinatus Beer Spoilage Bacteria by Using the Polymerase Chain Reaction." Journal of Food Protection 60, no. 12 (1997): 1571–73. http://dx.doi.org/10.4315/0362-028x-60.12.1571.

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Anaerobic bacteria of the genus Pectinatus cause beer spoilage by producing off flavors and turbidity. In unpasteurized beer even a small initial amount of contamination is likely to lead to a defective product. Detection of Pectinatus by traditional microbiological techniques is time-consuming and not practical as a preventive product control measure. In this paper Pectinatus-specific primers capable of discriminating among other beer contaminants by polymerase chain reaction are described. The present procedure, which includes the isolation of DNA from the contaminated beer sample, the polym

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STRAUB, JOACHIM A., CHRISTIAN HERTEL, and WALTER P. HAMMES. "A 23S rDNA-Targeted Polymerase Chain Reaction–Based System for Detection of Staphylococcus aureus in Meat Starter Cultures and Dairy Products." Journal of Food Protection 62, no. 10 (1999): 1150–56. http://dx.doi.org/10.4315/0362-028x-62.10.1150.

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A polymerase chain reaction–based system for detection of Staphylococcus aureus was developed. The system consisted of the following components: (i) selective enrichment, (ii) DNA isolation, (iii) amplification of DNA with primers targeted against the 23S rRNA gene, and (iv) evaluation of the specificity of the polymerase chain reaction by Southern hybridization and nested polymerase chain reaction. The method achieved a high degree of sensitivity and unambiguity as required for the detection of contaminants in food starter preparations. The method permitted detection of Staphylococcus aureus

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Blažková, M., M. Koets, J. H Wichers, A. van Amerongen, L. Fukal, and P. Rauch. "Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food." Czech Journal of Food Sciences 27, Special Issue 1 (2009): S350—S353. http://dx.doi.org/10.17221/959-cjfs.

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Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the pr

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Iancu, Vasile Ion, Jana Petre, Toma Galaon, et al. "LIQUID PHASE CHROMATOGRAPHIC METHODS APPLIED TO DETERMINE EMERGING CONTAMINANTS IN ENVIRONMENTAL SAMPLES." Romanian Journal of Ecology & Environmental Chemistry 2, no. 1 (2020): 11–20. http://dx.doi.org/10.21698/rjeec.2020.102.

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Emerging contaminants are a heterogeneous group of chemicals that include daily personal care products and pharmaceuticals (PPCPs), flame retardants, endocrine-disrupting chemicals (EDCs) and nanoparticles (NPs) present in environment which are unregulated. In this review, we present the methods of analysis conducted by INCD-ECOIND regarding some classes of emerging contaminants (neonicotinoid pesticides, beta-blocker drugs) that are not regulated by the legislation, in different types of environmental samples (wastewater, surface water). The present review presents the selective solid-phase e

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Kozhevnikova, Daria, Vasiliy Chernyshev, and Alexey Yashchenok. "Progress in Isolation and Molecular Profiling of Small Extracellular Vesicles via Bead-Assisted Platforms." Biosensors 13, no. 7 (2023): 688. http://dx.doi.org/10.3390/bios13070688.

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Tremendous interest in research of small extracellular vesicles (sEVs) is driven by the participation of vesicles in a number of biological processes in the human body. Being released by almost all cells of the body, sEVs present in complex bodily fluids form the so-called intercellular communication network. The isolation and profiling of individual fractions of sEVs secreted by pathological cells are significant in revealing their physiological functions and clinical importance. Traditional methods for isolation and purification of sEVs from bodily fluids are facing a number of challenges, s

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Przybylska, Anna, Grzegorz Bazylak, Robert Kosicki, et al. "Advantageous Extraction, Cleanup, and UHPLC-MS/MS Detection of Patulin Mycotoxin in Dietary Supplements and Herbal Blends Containing Hawberry from Crataegus spp." Journal of Analytical Methods in Chemistry 2019 (February 6, 2019): 1–13. http://dx.doi.org/10.1155/2019/2159097.

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Patulin (PAT) is a highly genotoxic mycotoxin still found as the common contaminant of various kinds of spoiled fruits and related commodities which are often endorsed as the health-enhancing products. Thus, a fast and convenient liquid-solid extraction followed by a solid-phase cleanup with the MycoSep®228 AflaPat multifunctional column was used for the highly efficient isolation of PAT with an average recovery of 112.7% from commercial dietary supplements and herbal blends formulated with dried hawberry. Analysis of the PAT content was carried out using gradient elution with a Synergi Polar

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Parkinson, Don-Roger, and Julian M. Dust. "Overview of the current status of sediment chemical analysis: trends in analytical techniques." Environmental Reviews 18, NA (2010): 37–59. http://dx.doi.org/10.1139/a09-015.

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This article reviews selected techniques and current trends in the analysis of contaminants in sediments since the year 2000. Because of the variety of anthropogenic target analytes encountered in sediments, the monograph is separated into inorganic and organic subsections. Practical aspects, including advances in: analysis of standards, biological methods, instrumental methods, modeling aspects, sample preparation and extraction methods, and speciation techniques are discussed. The sediment matrices are complex and require an integrated approach encompassing sampling, preparation, extraction,

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Antonopoulos, Dionysios, Irene Tsilioni, Sophia Tsiara, et al. "ExoProK: A Practical Method for the Isolation of Small Extracellular Vesicles from Pleural Effusions." Methods and Protocols 4, no. 2 (2021): 31. http://dx.doi.org/10.3390/mps4020031.

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Extracellular vesicles (EVs) are cell-secreted, lipid membrane-enclosed nanoparticles without functional nucleus. EV is a general term that includes various subtypes of particles named microvesicles, microparticles, ectosomes or exosomes. EVs transfer RNA, DNA and protein cargo between proximal and distant cells and tissues, thus constituting an organism-wide signal transduction network. Pathological tissues secrete EVs that differ in their cargo composition compared to their healthy counterparts. The detection of biomarkers in EVs from biological fluids may aid the diagnosis of disease and/or

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Gavrilă, Ana-Mihaela, Mariana Ioniță, and Gabriela Toader. "Recent Advances in Molecularly Imprinted Polymers and Emerging Polymeric Technologies for Hazardous Compounds." Polymers 17, no. 8 (2025): 1092. https://doi.org/10.3390/polym17081092.

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Addressing hazards from dangerous pollutants requires specialized techniques and risk-control strategies, including detection, neutralization and disposal of contaminants. Smart polymers, designed for specific contaminants, provide powerful solutions for hazardous compound challenges. Their remarkable performance capabilities and potential applications present exciting opportunities for further exploration and development in this field. This editorial aims to provide a comprehensive overview of smart materials with unique features and emerging polymeric technologies that are being developed fo

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Ríos, Melina Ocampo, Paula Fernández, and Marcelo Carmona. "Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique." Fitopatologia Brasileira 32, no. 5 (2007): 415–18. http://dx.doi.org/10.1590/s0100-41582007000500007.

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Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Mal

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Han, Jung Y., Michael Yeh, and Don L. DeVoe. "Nanogap traps for passive bacteria concentration and single-point confocal Raman spectroscopy." Biomicrofluidics 17, no. 2 (2023): 024101. http://dx.doi.org/10.1063/5.0142118.

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A microfluidic device enabling the isolation and concentration of bacteria for analysis by confocal Raman spectroscopy is presented. The glass-on-silicon device employs a tapered chamber surrounded by a 500 nm gap that serves to concentrate cells at the chamber apex during sample perfusion. The sub-micrometer gap retains bacteria by size exclusion while allowing smaller contaminants to pass unimpeded. Concentrating bacteria within the fixed volume enables the use of single-point confocal Raman detection for the rapid acquisition of spectral signatures for bacteria identification. The technolog

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Banu, S. Amitha, Shubham Saini, Khan Sharun, et al. "Isolation and Characterization of Bacterial Contaminants from Bone Marrow-Derived Mesenchymal Stem Cell Cultures." Journal of Pure and Applied Microbiology 18, no. 1 (2024): 653–61. http://dx.doi.org/10.22207/jpam.18.1.50.

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This study aimed to assess and manage bacterial contamination in multiple batches of mesenchymal stem cell (MSC) cultures derived from rabbit bone marrow. Routine visual inspection and microscopic examination were employed for the detection of the contaminated cultures. The contaminated cultures were inoculated on Nutrient agar and multiple isolated colonies were subjected to Gram staining and biochemical characterization. Further, molecular identification of the bacterial isolates was performed using polymerase chain reaction. The determination of antibiotic susceptibility patterns was conduc

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Holz, Nathalie, Nils Wax, Boris A. Illarionov, Margarita Iskhakova, and Markus Fischer. "Food Authentication: The Detection of Arbutus unedo and Olea europaea Leaves as an Admixture of Oregano Using LAMP- and Duplex LAMP-Based Test Systems with Lateral-Flow Assays." Agriculture 14, no. 4 (2024): 597. http://dx.doi.org/10.3390/agriculture14040597.

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The Mediterranean herb oregano is one of the most frequently adulterated foods. Often morphologically similar leaf material is used as a filler, which can generally be detected using DNA-based methods. Loop-mediated isothermal amplification (LAMP) has high potential for point-of-care testing as it requires only a simple device for sample incubation and is less sensitive to inhibition by co-isolated metabolites compared to conventional PCRs (polymerase chain reactions). In this work, we have developed two LAMP assays for the specific detection of the adulterants olive (Olea europaea) and strawb

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Zhang, Liding, Xuewei Du, Congjie Chen, et al. "Development and Characterization of Double-Antibody Sandwich ELISA for Detection of Zika Virus Infection." Viruses 10, no. 11 (2018): 634. http://dx.doi.org/10.3390/v10110634.

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Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that can cause severe disease, including congenital birth defect and Guillain−Barré syndrome during pregnancy. Although, several molecular diagnostic methods have been developed to detect the ZIKV, these methods pose challenges as they cannot detect early viral infection. Furthermore, these methods require the extraction of RNA, which is easy to contaminate. Nonstructural protein 1 (NS1) is an important biomarker for early diagnosis of the virus, and the detection methods associated with the NS1 protein have recently been reporte

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Hassan, Md Kamrul, Munalisa Poppy, Tanvir Ahamed, et al. "Bacterial Flora Isolated From Dried Fishes Sold at Retail Markets Within Dhaka City Corporation of Bangladesh." Research in Agriculture Livestock and Fisheries 8, no. 1 (2021): 109–15. http://dx.doi.org/10.3329/ralf.v8i1.53272.

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Along with other contaminants, bacterial contamination in the dried fishes is a common issue which severely affects the quality of cured fishes. Hence, the present study was aimed to isolate and identify different bacterial flora contaminating different dried fishes, sold at different retail markets within Dhaka Metropolitan city, Dhaka, Bangladesh. A total of 25 different dried fishes were collected from Dhaka North city corporation area (Town Hall kacha bazar, Krishi market, and Mohammadpur kacha bazar) and Dhaka South city corporation area (New market kacha bazar, and Jatrabari chowrasta ba

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Rokhim, Saiku, Inggrit Tyautari, M. Aliffiyan Firmansyah, and Yuanita Rachmawati. "End Point Polymerase Chain Reaction for Porcine Detection on Food Product of UIN Sunan Ampel Surabaya Canteen." Indonesian Journal of Halal Research 3, no. 1 (2021): 21–26. http://dx.doi.org/10.15575/ijhar.v3i1.11149.

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Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PC

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MOIO, LUIGI, CRISTINA MARCHISANO та FRANCESCO ADDEO. "Isolation of specific oligoclonal antibodies against bovine αs1-casein by FPLC tandem immunoaffinity of the polyclonal antibodies". Journal of Dairy Research 65, № 3 (1998): 515–20. http://dx.doi.org/10.1017/s002202999800291x.

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Polyclonal antibodies specifically directed towards native casein fractions have been recently used to solve some analytical problems such as localization of casein antigenic sites (Otani et al. 1985; Ametani et al. 1987), identification of casein variants (Moio et al. 1989a; Chianese et al. 1992), detection of bovine casein adulterating goats', ewes' and water-buffalo milk and cheese (Elbertzhagen & Wenzel, 1982; Bernhauer et al. 1983; Aranda et al. 1988; Moio et al. 1992; Rolland et al. 1993, 1995; Addeo et al. 1995b), evaluation of the efficiency of chromatographic fractionation of case

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Vahkal, Brett, Jamie Kraft, Emanuela Ferretti, Minyoung Chung, Jean-François Beaulieu, and Illimar Altosaar. "Review of Methodological Approaches to Human Milk Small Extracellular Vesicle Proteomics." Biomolecules 11, no. 6 (2021): 833. http://dx.doi.org/10.3390/biom11060833.

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Proteomics can map extracellular vesicles (EVs), including exosomes, across disease states between organisms and cell types. Due to the diverse origin and cargo of EVs, tailoring methodological and analytical techniques can support the reproducibility of results. Proteomics scans are sensitive to in-sample contaminants, which can be retained during EV isolation procedures. Contaminants can also arise from the biological origin of exosomes, such as the lipid-rich environment in human milk. Human milk (HM) EVs and exosomes are emerging as a research interest in health and disease, though the exp

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Putri, Vita Septiana Sulaiman, Faturrahman Faturrahman, Sarkono Sarkono, Galuh Tresnani, and Bambang Fajar Suryadi. "Detection of Bacterial Contaminants and Identification of Salmonella Sp. in Cilok Snack in Selaparang District, Mataram." Samota Journal of Biological Sciences 2, no. 1 (2023): 1–6. https://doi.org/10.29303/sjbios.v2i1.2885.

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Cilok is a boiled snack made from starch with or without a mixture of minced meat, has a savory and chewy taste. Cilok is one of the favorite snacks in Indonesia. Most cilok sellers sell their cilok right on the side of the main road for hours. This allows cilok that are sold to be contaminated with various bacteria. This study aims to calculate the Total Plate Count (TPC) of bacteria and to identify Salmonella sp. in cilok and its sauce in Selaparang District, Mataram City. This study used quantitative method in the form of calculating the TPC of the bacteria and qualitative method in the for

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