Relevant bibliographies by topics / Conventional staining

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  2. Dissertations / Theses
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  4. Conference papers

Academic literature on the topic 'Conventional staining'

Author: Grafiati
Published: 4 June 2021
Last updated: 19 June 2025

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Journal articles on the topic "Conventional staining"

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Merghani A Elmahdi, Faris, Thabat Ibrahim Alrfaie, Ehdaa Mahmmoud Alsubaihi, et al. "Comparative Study of Conventional Staining Techniques in Cytology." International Journal of Science and Research (IJSR) 11, no. 5 (2022): 876–79. http://dx.doi.org/10.21275/sr22514114506.

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Sawicki, Wojciech, and Stanislaw Moskalewski. "Hoechst 33342 Staining Coupled with Conventional Histological Technique." Stain Technology 64, no. 4 (1989): 191–96. http://dx.doi.org/10.3109/10520298909106998.

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Jellinger, K. "A conventional approach to dorsal raphe nucleus staining." Neuropathology and Applied Neurobiology 26, no. 6 (2000): 571–72. http://dx.doi.org/10.1046/j.1365-2990.2000.00285-2.x.

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Raja, Veena, ChinnayaSubramaniyamBabuRajendra Prasad, and Lavanya Rajagopal. "Rapid Cytodiagnosis By Different Staining Techniques In Comparison With Conventional Stains In Thyroid Cytology." Annals of Pathology and Laboratory Medicine 4, no. 6 (2017): A761—A767. http://dx.doi.org/10.21276/apalm.1621.

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Mary Ranadheer, Jamullamudi Bhaskar, B. V. Sivamma, P. Ratna kumari, and Yona Manchikalapati. "A Comparative analysis of Conventional Staining methods versus Fluorescent staining in detection of pulmonary tuberculosis." Indian Journal of Medical Microbiology 39 (September 2021): S93—S94. http://dx.doi.org/10.1016/j.ijmmb.2021.08.324.

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Parise, Patricia P., Rita M. P. Avancini, and Shirlei M. Recco-Pimentel. "Karyotypic characterization ofMuscina stabulans(Fallen) (Diptera: Muscidae) using conventional staining, silver staining and C-banding." Caryologia 49, no. 1 (1996): 13–20. http://dx.doi.org/10.1080/00087114.1996.10797345.

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Panneerselvam, Karthika, KRaghavendhar Karthik, Ramya Ramadoss, ARamesh Kumar, and K. Rajkumar. "Rapid economical acetic acid Papanicolaou staining procedure versus conventional staining procedure in normal oral mucosa." Journal of Oral and Maxillofacial Pathology 26, no. 2 (2022): 285. http://dx.doi.org/10.4103/jomfp.jomfp_135_20.

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Osipov, Andreyan, Ekaterina Arkhangelskaya, Alexei Vinokurov, Nadezhda Smetaninа, Alex Zhavoronkov, and Dmitry Klokov. "DNA Comet Giemsa Staining for Conventional Bright-Field Microscopy." International Journal of Molecular Sciences 15, no. 4 (2014): 6086–95. http://dx.doi.org/10.3390/ijms15046086.

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Berghorn, K. A., J. H. Bonnett, and G. E. Hoffman. "cFos immunoreactivity is enhanced with biotin amplification." Journal of Histochemistry & Cytochemistry 42, no. 12 (1994): 1635–42. http://dx.doi.org/10.1177/42.12.7983364.

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Through modification of the protocol by Adams, we developed a biotin amplification procedure for immunofluorescence staining of immediate early gene proteins and also applied biotin amplification for metal enhancement of diaminobenzidine staining in an immunoperoxidase protocol. Commercially available anti-cFos antisera were used to compare conventional "Elite" avidin-biotin complex reactions with biotin amplification reactions (visualized with peroxidase staining or streptavidin-Texas Red fluorescence). Biotin amplification and peroxidase staining (with or without nickel salts) enabled detect
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Hye, Rizwana Abdul, Bindiya Gisuthan, and Indira Kariveettil. "A Comparative Study between Conventional and Modified Leishman Stain." International Journal of Research and Review 8, no. 2 (2021): 5–12. http://dx.doi.org/10.52403/ijrr.20210202.

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Introduction: Leishman stain has been used as the stain of choice for peripheral blood films since many decades .But it has a disadvantage of consuming 15 minutes for the procedure alone thereby increasing the turn around time of peripheral smear reporting. In this study modified Leishman stain was made by adding phenol to conventional Leishman to reduce the staining time to 3 minutes without interfering with the quality of stain. Aim: To study the quality of modified Leishman stain in comparison with conventional preparation on peripheral blood smears. Materials and Methods: The present cross
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Dissertations / Theses on the topic "Conventional staining"

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SILVA, Maria Luiza da. "Citogenética em espécies do gênero Eichhornia Kunth, Pontederiaceae Kunth." Universidade Federal Rural de Pernambuco, 2014. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4891.

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Submitted by (edna.saturno@ufrpe.br) on 2016-06-29T13:52:35Z No. of bitstreams: 1 Maria Luiza da Silva.pdf: 1369807 bytes, checksum: 27a12fb618790d4807325a30dfc6fc12 (MD5)<br>Made available in DSpace on 2016-06-29T13:52:35Z (GMT). No. of bitstreams: 1 Maria Luiza da Silva.pdf: 1369807 bytes, checksum: 27a12fb618790d4807325a30dfc6fc12 (MD5) Previous issue date: 2014-02-10<br>Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq<br>Eichhornia is a Neotropical genus belonging to the family Pontederiaceae. It occurs in aquatic environments with outstanding ecologi
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Woerner, Rachel Anna. "Development of cryopreparation methods and improved staining techniques for transmission electron microscopy of wool." Thesis, Queensland University of Technology, 2002.

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Book chapters on the topic "Conventional staining"

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Ellis, E. Ann. "Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and En Bloc Stains." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-776-1_4.

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"Staining and Banding Techniques for Conventional Cytogenetic Studies 1." In Medical Cytogenetics. CRC Press, 2000. http://dx.doi.org/10.1201/9781482292992-5.

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Ingham, C. A. "Immunocytochemistry II: post-embedding staining." In Experimental Neuroanatomy. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199633265.003.0006.

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Abstract Post-embedding immunocytochemistry involves carrying out immuno cytochemical techniques on tissue which has been fixed, dehydrated, and embedded in a material suitable for cutting sections for subsequent light or electron microscopy, (e.g. wax, celloidin, resin). It is beyond the scope of this chapter to discuss all the available techniques and so it will concentrate on post-embedding methods applied to material which has been prepared in the conventional manner for electron microscopy, including post-fixation with osmium tetroxide and embedding in epoxy resin. Many antigens do not survive this treatment; their antigenicity may be affected by osmium tetroxide treatment, by resin infiltration (possibly due to interactions of the antigen with epoxy groups), and by heat (1-3). However, amino acids such as -y aminobutyric acid (GABA), glutamate, aspartate, taurine, glycine, and glutamine have been successfully localized using these methods (4).
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Ibrahim, Merdol, and Guy Orchard. "Immunocytochemical techniques." In Histopathology, edited by Guy Orchard and Brian Nation. Oxford University Press, 2017. http://dx.doi.org/10.1093/hesc/9780198717331.003.0008.

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This chapter addresses immunocytochemical techniques and their role in diagnostic histopathology. Immunocytochemistry (ICC) is used as a second string of investigations following conventional staining techniques. The role of the primary investigations is to demonstrate the morphological appearance of the cell and tissue architecture. This enables identification of the disease process, and in a substantial number of cases is adequate to formulate a final diagnosis. However, the demonstration of morphological detail alone may not enable a diagnosis to be made in all cases, and in such instances an assessment of the cell marker (antigen) expression on the cell membrane, cytoplasm, or nucleus is required. This provides selective information that can be used to define certain cell types. This is the role of ICC, which, in some instances, is closely allied to molecular diagnostics. The chapter then details the process of tissue preparation and antigen retrieval.
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Wackerbauer, Karl, Michel Camielo, and Rolf Hardt. "Video/Image analysis system for the calcofluor malt modification method." In European Brewery Convention. Oxford University PressOxford, 1993. http://dx.doi.org/10.1093/oso/9780199634668.003.0053.

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Abstract Sample preparation is decisive for the quality of the results in the analysis of malt modification. The sanding and staining procedures have been optimized in order to get best contrast between fluorescent and non¬ fluorescent parts of the malt grains. By this the evaluation of the prepared clayblocks becomes much easier and the analysis results show better reproducibility.
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Davies, Derek C., and Bryan D. Youngt. "Chromosome analysis and sorting by flow cytometry." In Flow Cytometry. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199638253.003.0012.

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Abstract Many types of cancer and genetic disease are characterized by chromosomal abnormalities. These can be detected by conventional cytogenetics, which involves photographing banded chromosomes on metaphase spreads. Although this is a widely used technique in haematology, oncology, and pre-natal diagnosis, it is a time-consuming process that relies heavily on the skill and experience of the cytogeneticist. In addition, in cancerous cells, the sometimes complex karyotypes encountered mean that such analysis is often extremely difficult. An alternative approach is to prepare a monodispersed suspension of chromosomes, stain with one or two fluorescent DNA dyes, and pass them through a flow cytometer. The intensity of the fluorescence signal from each chromosome is recorded, the values being dependent on their DNA content. In single-colour analysis, a non-base-specific dye such as ethidium bromide is used. Accumulating data from 50000 chromosomes and presenting this as a histo gram of fluorescence intensity produces a distinctive species-specific pattern of peaks. However, not all chromosomes appear as a single peak. An improvement is to use bivariate analysis that exploits the base-pair binding preferences of DNA specific dyes: usually Hoechst 33258 (which has an adenine/thymidine (AT) binding preference) in combination with chromomycin A3 (which has a guanine/cytosine (GC) binding preference). The intensity of staining with these fluorochromes is dependent not only on the DNA content but also on the base composition of each chromosome. The data accumulated can be presented as an isometric plot or, better, as a dot plot or contour map (see Figure 1). The bivariate plot resolves all the human chromosomes as separate peaks, with the exception of chromosomes 9, 10, 11, and 12.
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Peladan, F., and R. Leitz. "New method for the differential staining of dead and living cells of yeasts and bacteria." In European brewery convention. Oxford University PressOxford, 1991. http://dx.doi.org/10.1093/oso/9780199632831.003.0059.

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Abstract The proposed method uses two fluorescent dyes. It allows to count, in 30’ and with a hight sensitivity (1 cell/100 ml), dead and alive yeasts and bacteria. In other respects, none of the described problems arise and this method could be used all along the beer process.
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Contag, Christopher H. "Revealing the Subtleties of Disease and the Nuances of the Therapeutic Response with Optical Reporter Genes." In Biomedical Optical Imaging. Oxford University PressNew York, NY, 2009. http://dx.doi.org/10.1093/oso/9780195150445.003.0015.

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Abstract The use of optical imaging to study biology in animal models and to detect markers of disease in humans provides a window through which we can view features of disease that are either not detectable by conventional approaches or that can be detected more efficiently with optics. Moreover, with optics we can often appreciate patterns in biological processes that would be otherwise overlooked or missed. Because the nuances of disease mechanisms and the subtleties of the response to therapy are key to understanding and resolving disease, imaging has become an essential tool for revealing pathogenic mechanisms and for developing effective therapeutic strategies. Optical imaging tools comprise a key set of approaches for the study of biology in animal models, and are emerging as powerful methods for early disease detection, staging of progression, and assessing response to therapy in the clinic. Sensitive and rapid imaging methods based on the optical properties of tissues or the detection of reporter proteins and dyes provide real-time information that will contribute to our ability to unravel the mechanisms of disease, enable us to intervene with treatment strategies, and allow us to assess therapeutic outcomes. Because optical imaging methods cover a range of scales, with resolutions from microns to centimeters, the preclinical and clinical applications are far-reaching, with great potential for changing the way these studies are performed. The clinical utility of optical imaging, however, is limited to a narrow set of applications in which the depth of penetration of light is sufficient to interrogate the intended target in mammalian tissues. These intended targets could be biological structures or molecules that are intrinsic to the tissue, or exogenous reagents that can label cells or stain tissues. The small number of approved optical contrast agents for human use further confines optical imaging in the clinic to specific application areas. In contrast, in preclinical studies the applications are limitless because the study subjects are typically small laboratory rodents in which many tissues and organs are accessible to optical tools. In addition, the cells and tissues in laboratory animals are amenable to staining with a range of exogenous dyes and may even be manipulated genetically to express optical reporters. Perhaps the greatest impact that optical imaging will have on human health is by providing a greater understanding of mammalian biology and more rapid evaluation of new therapies in laboratory animals.
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Conference papers on the topic "Conventional staining"

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Sabbir, Md Ahsan, Saiada Fuadi Fancy, Kingsley Lau, and Dale DeFord. "Assessment of Novel Coatings in Comparison to Conventional Zinc-Rich Coating for Bridge Application." In SSPC 2018. SSPC, 2018. https://doi.org/10.5006/s2018-00020.

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Abstract Coatings are widely used to mitigate corrosion of structural steel in aggressive humid environments. Zinc-rich primer three-coat paint systems are widely used to mitigate corrosion of steel bridges. However, the associated costs of its required maintenance are high. As part of a research program, chemically bonded phosphate ceramics (CBPC), thermal diffusion galvanizing (TDG) and metallizing coatings along with the current 3-coat systems were exposed in outdoor conditions for up to 2 years and in salt-fog exposure for up to 14,600 hours. Testing included visual photo-documentation, co
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Kearns, J. R., L. R. Barkowski, M. J. Johnson, and P. J. Pavlik. "The Corrosion and Appearance of Stainless Steels in the Atmosphere." In CORROSION 1987. NACE International, 1987. https://doi.org/10.5006/c1987-87418.

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Abstract The resistance of 38 types of stainless steel to degradation in the atmosphere was directly related to alloy chromium and molybdenum contents by spectrocol-ormetric measurements and a visual rating system. Only AISI Type 410 showed any signs of corrosion in a semirural environment. Significant corrosion product staining occurred on all non-molybdenum-bearing stainless grades that had been exposed for 2 to 15 years at the 250-m lot on Kure Beach, N.C. Stainless grades that had been sensitized by an autogenous welding operation were susceptible to preferential corrosion at weld and heat
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Chaubey, Shivam Kumar, Mohit Rathor, Rupen Tamang, Biplob Koch, and Rakesh Kumar Singh. "Enhanced live cell imaging through polarization digital holographic microscope." In JSAP-Optica Joint Symposia. Optica Publishing Group, 2024. https://doi.org/10.1364/jsapo.2024.16p_a37_2.

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Polarization is significant in understanding complex optical behaviors and revealing properties that conventional methods often miss. However, traditional polarization measurement techniques typically involve multiple captures, which are not ideal for live cell imaging. This study presents our advancements in developing a polarization digital holographic microscope (PDHM) tailored for spatially resolved, label-free imaging. Our approach focuses on single-shot polarization imaging, which significantly enhances the feasibility of real-time observations [1]. We demonstrate the practical applicati
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Ting, S. L., P. K. Tan, T. T. Yu, P. T. Ng, and C. Q. Chen. "Using FIB Grooving to Prepare Top-down-Nanoprobed Sample for Site-Specific Cross-Sectional Nanoprobing Analysis." In ISTFA 2024. ASM International, 2024. http://dx.doi.org/10.31399/asm.cp.istfa2024p0411.

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Abstract Cross-sectional analysis plays a crucial role in failure analysis for the identification of root causes associated with implants or junction profiles. Traditionally, this step involves junction staining. Recently, Electron Beam Induced Current (EBIC) analysis has emerged as a valuable alternative, offering the key advantage of visualizing various implantations and junction profiles through non-chemical means. This paper presents an innovative sample preparation technique for cross-sectional EBIC analysis, incorporating an additional step of FIB (Focused Ion Beam) grooving at the targe
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Tanaka, T., T. Ishikawa, K. Numayama-Tsuruta, et al. "Collection of Cancer Cells From Blood Samples Using Inertial Migration Forces." In ASME 2011 6th Frontiers in Biomedical Devices Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/biomed2011-66034.

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Circulating Tumor Cell (CTC) test has been widely adopted for evaluating the prognosis or cure effect of breast cancer. In this test, situations of breast cancer patients are evaluated by counting the number of CTCs in peripheral blood samples. Since conventional method needs fluorescent staining to count CTCs, there are problems of high costs and long process time.
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Bini, A., R. Mesa-Tejada, J. Fenoglio, B. Kudryk, and K. L. Kaplan. "SPECIFIC DETECTION OF FIBRIN IN HUMAN TISSUES BY A NEW IMMUNOHISTOCHEMICAL TECHNIQUE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643316.

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Human biopsy (30), surgical (50) and autopsy (14) specimens of different embryonic origin (skin, blood vessel, kidney, lymph nodes, prostate, lung, liver, and intestine) were stained by the avidin-biotin complex immunoperoxidase technique (ABC-IP) with monoclonal antibodies (MAbs). MAb T2G1 (recognizes 315-42 and detects fibrin II in tissues), MAb I8C6 (recognizes BS1-42 and indicates fibrinogen and fibrin I), MAb GC4 (specific for fragments D and D-D), and a polyclonal antiserum for fibrinogen. The method can be applied to frozen or Boilin’s fixed paraffin embedded tissues with good preservat
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Nakarada, Đura, T. Tomašević, A. Dragićević, Dijana Mitić, Aleksandar Savić, and Miloš Mojović. "ELECTROCHEMICAL AND EPR CHARACTERIZATION OF STEM CELLS FOR APPLICATIONS IN REGENERATIVE MEDICINE." In 17th International Conference on Fundamental and Applied Aspects of Physical Chemistry. Society of Physical Chemists of Serbia, 2024. https://doi.org/10.46793/phys.chem24i.239n.

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Stem cell research has emerged as a promising course for regenerative medicine. A critical aspect of stem cell applications is the accurate assessment of cell viability and redox status. Traditional methods for evaluating cell viability, such as morphological assessment, cell counting, viability staining, metabolic activity assays, apoptosis detection, and functional assays, are often time- consuming, labor-intensive, and costly. In this study, we explore the use of electrochemical techniques, specifically cyclic voltammetry and electrochemical impedance spectroscopy, to provide a rapid and ef
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Whited, Bryce M., Matthias C. Hofmann, Chris G. Rylander, et al. "Non-Destructive Real-Time Imaging of Cell Seeded Tissue Engineered Scaffolds." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53721.

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The use of tissue engineered scaffolds in combination with progenitor cells has emerged as a promising strategy to restore or replace tissues damaged by disease or trauma. In addition to being biocompatible and exhibiting appropriate mechanical properties, scaffolds must be designed to sustain cell attachment, proliferation, and differentiation to ultimately produce the desired tissue once implanted in the patient [1]. Conventional techniques used to assess successful scaffold design include cell viability stains, DNA assays, and histological sectioning/staining. While significant information
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Tung, Yen-Ting, and Gou-Jen Wang. "In Vitro Development of Microvessels Using a Scaffold of Cylindrical PLGA." In ASME 2016 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/detc2016-59550.

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The microvascular network is a simple but critical system that is responsible for various important biological mechanisms in the bodies of all animals. The ability to generate a functional microvessel in vitro not only makes it possible to engineer vital tissue of considerable size but also serves as a platform for biomedical studies. In this study, we propose a simple method for fabricating customized cylinder micro-scaffolds for the in vitro development of microvascular networks. By integrating micro-electro-mechanical systems techniques with thermal reflow, we design and fabricate a micro-s
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García-Aguilar, Karol, Yareth Gopar-Cuevas, María-de-Lourdes Chávez-Briones, Marta Ortega-Martínez, and Gilberto Jaramillo-Rangel. "Optimization of Gram Stain for Its Application in Tissues." In International Medicine and Health Sciences Congress. ECER, 2024. https://doi.org/10.53375/imhsc.2024.30.

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Gram stain remains one of the most universally used techniques for microbiological diagnosis. In clinical practice, situations may arise in which the establishment of the etiology of certain bacterial infections is limited to histological findings. Although conventional Gram stain works very well to identify bacteria in smears from liquid samples, its application is not appropriate for tissue sections, because connective tissue and other elements of biopsies stain easily and nonspecifically with the dyes used in the technique, which complicates the visualization of bacteria. Therefore, the obj
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