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Journal articles on the topic 'Copper Uptake'

Author: Grafiati

Published: 5 June 2025

Last updated: 15 July 2025

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1

Abramson, R. G., and M. S. Lipkowitz. "Carrier-mediated concentrative urate transport in rat renal membrane vesicles." American Journal of Physiology-Renal Physiology 248, no. 4 (1985): F574—F584. http://dx.doi.org/10.1152/ajprenal.1985.248.4.f574.

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[2-14C]Urate uptake and efflux were studied in brush border and basolateral membrane vesicles of rat renal cortex that were exposed to 20 microM copper chloride. In the presence of inwardly directed NaCl gradients urate uptake was maintained at levels in excess of chemical equilibrium. Comparison of glucose and chloride uptakes revealed that equilibrium glucose uptake was not affected by copper, but chloride failed to reach equilibrium in copper-exposed vesicles. It is suggested that the persistence of an electrolyte gradient could provide a driving force to raise the concentration of free int

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2

Tsekova, Kohshka, Vera Dentcheva, and Maria Ianis. "Copper Uptake by Penicillium brevicompactum." Zeitschrift für Naturforschung C 56, no. 9-10 (2001): 803–5. http://dx.doi.org/10.1515/znc-2001-9-1019.

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Abstract Penicillium brevicompactum, Copper Uptake, Kinetics The copper binding properties of Penicillium brevicompactum biomass were influenced by growth phase of mycelium and concentration of copper in reaction mixtures. The efficiency of copper uptake increased with growth time and was largest at the mid-logarithmic growth phase. The time course of copper uptake was biphasic. Double reciprocal plots of absorption velocity of copper vs. copper concentration gave straight lines at concentration between 0.5 to 4 mᴍ . The apparent affinity of copper to the biomass of the stationary growth phase

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3

HASSETT, Richard, David R. DIX, David J. EIDE, and Daniel J. KOSMAN. "The Fe(II) permease Fet4p functions as a low affinity copper transporter and supports normal copper trafficking in Saccharomyces cerevisiae." Biochemical Journal 351, no. 2 (2000): 477–84. http://dx.doi.org/10.1042/bj3510477.

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The plasma-membrane of Saccharomycescerevisiae contains high affinity permeases for Cu(I) and Fe(II). A low affinity Fe(II) permease has also been identified, designated Fet4p. A corresponding low affinity copper permease has not been characterized, although yeast cells that lack high affinity copper uptake do accumulate this metal ion. We demonstrate in the present study that Fet4p can function as a low affinity copper permease. Copper is a non-competitive inhibitor of 55Fe uptake through Fet4p (Ki = 22µM). Fet4p-dependent 67Cu uptake was kinetically characterized, with Km and Vmax values of

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4

C.Go, Jerwin Lawrence, Cynthia F. Madrazo, Aileen H. Orbecido, Ma Ellenita G. de Castro, and Lawrence P. Belo. "Copper uptake potential of Philippine giant bamboo (Dendrocalamus asper) under varied initial copper concentration, water hardness and pH." MATEC Web of Conferences 268 (2019): 06005. http://dx.doi.org/10.1051/matecconf/201926806005.

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Copper is a commonly used metal in construction, engineering, agriculture and water treatment. Consequently, increased copper concentrations resulting in adverse environmental effects is inevitable. Phytoremediation using Dendrocalamus asper or Philippine giant bamboo (PGB) is a viable option for treatment of copper-contaminated media, but their copper uptake potential remains largely unexplored. As such, the copper uptake of PGB was evaluated under varying environmental conditions, namely initial copper concentration, water hardness and pH.Six-month old propagules were planted in artificially

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5

Grosell, Martin, and Chris M. Wood. "Copper uptake across rainbow trout gills." Journal of Experimental Biology 205, no. 8 (2002): 1179–88. http://dx.doi.org/10.1242/jeb.205.8.1179.

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SUMMARYSeveral components of branchial copper uptake were identified in juvenile freshwater rainbow trout (Oncorhynchus mykiss) using 64Cu. On the basis of competitive interactions between sodium and copper uptake,inhibition of copper uptake by a proton pump inhibitor (bafilomycin A1, 2μmol l-1) and a Na+ channel blocker (phenamil, 100μmol l-1), it appears that a proportion of the branchial copper uptake occurs via an apical Na+ channel. This sodium-sensitive copper uptake demonstrates saturation kinetics, with a Km of 7.1 nmol l-1 and a Jmax of 21.2 pmol g-1 h-1, and is characterized by an IC

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6

Robson, AD, and K. Snowball. "The effect of chlorsulfuron on the uptake and utilization of copper and zinc in wheat." Australian Journal of Agricultural Research 41, no. 1 (1990): 19. http://dx.doi.org/10.1071/ar9900019.

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The effect of the herbicide chlorsulfuron on the uptake and utilization of copper and zinc by wheat was examined in a series of glasshouse experiments. Application of chlorsulfuron induced symptoms of both copper and zinc deficiencies, and decreased copper and zinc concentrations within shoots. Chlorsulfuron accentuated deficiencies of copper and zinc by decreasing uptake of the micronutrients rather than by affecting their utilization within the plant. Effects of chlorsulfuron in decreasing uptake of copper and zinc did not appear to be due to effects on root weight or root length. However, c

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7

Zerounian, Nora R., Carmen Redekosky, Rashmi Malpe, and Maria C. Linder. "Regulation of copper absorption by copper availability in the Caco-2 cell intestinal model." American Journal of Physiology-Gastrointestinal and Liver Physiology 284, no. 5 (2003): G739—G747. http://dx.doi.org/10.1152/ajpgi.00415.2002.

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Relatively little is known about the individual steps in intestinal copper absorption and whether or how they may be regulated. Polarized Caco-2 cell monolayers with tight junctions offer an already tested model in which to study intestinal metal transport. This model was used to examine potential effects of cellular copper availability on copper absorption. Uptake and transport were determined on application of64Cu(II) to the brush border. In the range of 0.2–2 μM, uptake was dose dependent and was ∼20% of dose/90 min. Overall transport of64Cu across the basolateral surface was ∼0.3%. When ce

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8

Gu, Wenyu, Muhammad Farhan Ul Haque, Bipin S. Baral, et al. "A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b." Applied and Environmental Microbiology 82, no. 6 (2016): 1917–23. http://dx.doi.org/10.1128/aem.03884-15.

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ABSTRACTMethanobactin, a small modified polypeptide synthesized by methanotrophs for copper uptake, has been found to be chromosomally encoded. The gene encoding the polypeptide precursor of methanobactin,mbnA, is part of a gene cluster that also includes several genes encoding proteins of unknown function (but speculated to be involved in methanobactin formation) as well asmbnT, which encodes a TonB-dependent transporter hypothesized to be responsible for methanobactin uptake. To determine ifmbnTis truly responsible for methanobactin uptake, a knockout was constructed inMethylosinus trichospo

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9

Moriya, Mizue, Yi-Hsuan Ho, Anne Grana та ін. "Copper is taken up efficiently from albumin and α2-macroglobulin by cultured human cells by more than one mechanism". American Journal of Physiology-Cell Physiology 295, № 3 (2008): C708—C721. http://dx.doi.org/10.1152/ajpcell.00029.2008.

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Ionic copper entering blood plasma binds tightly to albumin and the macroglobulin transcuprein. It then goes primarily to the liver and kidney except in lactation, where a large portion goes directly to the mammary gland. Little is known about how this copper is taken up from these plasma proteins. To examine this, the kinetics of uptake from purified human albumin and α2-macroglobulin, and the effects of inhibitors, were measured using human hepatic (HepG2) and mammary epithelial (PMC42) cell lines. At physiological concentrations (3–6 μM), both cell types took up copper from these proteins i

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10

Herd, S. M., J. Camakaris, R. Christofferson, P. Wookey, and D. M. Danks. "Uptake and efflux of copper-64 in Menkes'-disease and normal continuous lymphoid cell lines." Biochemical Journal 247, no. 2 (1987): 341–47. http://dx.doi.org/10.1042/bj2470341.

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The accumulation of copper over 2 h by normal lymphoid cells and those from Menkes'-disease patients (Menkes' cells) was found to be biphasic, with an initial phase of rapid uptake, an approach to steady state at around 40-60 min, followed by a further accumulation phase. The accumulation of copper was not diminished by the addition of a variety of metabolic inhibitors, suggesting that copper uptake is not an active process. The presence of carbonyl cyanide m-chlorophenylhydrazone in the culture medium stimulated the uptake and accumulation of copper in both normal and Menkes' cells to the sam

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11

Bertinato, Jesse, Eleonora Swist, Louise J. Plouffe, Stephen P. J. Brooks, and Mary R. L'Abbé. "Ctr2 is partially localized to the plasma membrane and stimulates copper uptake in COS-7 cells." Biochemical Journal 409, no. 3 (2008): 731–40. http://dx.doi.org/10.1042/bj20071025.

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Ctr1 (copper transporter 1) mediates high-affinity copper uptake. Ctr2 (copper transporter 2) shares sequence similarity with Ctr1, yet its function in mammalian cells is poorly understood. In African green monkey kidney COS-7 cells and rat tissues, Ctr2 migrated as a predominant band of ∼70 kDa and was most abundantly expressed in placenta and heart. A transiently expressed hCtr2–GFP (human Ctr2–green fluorescent protein) fusion protein and the endogenous Ctr2 in COS-7 cells were mainly localized to the outer membrane of cytoplasmic vesicles, but were also detected at the plasma membrane. Bio

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12

Southon, Adam, Mark A. Greenough, George Ganio, Ashley I. Bush, Richard Burke, and James Camakaris. "Presenilin Promotes Dietary Copper Uptake." PLoS ONE 8, no. 5 (2013): e62811. http://dx.doi.org/10.1371/journal.pone.0062811.

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13

Zimnicka, Adriana M., Kristin Ivy, and Jack H. Kaplan. "Acquisition of dietary copper: a role for anion transporters in intestinal apical copper uptake." American Journal of Physiology-Cell Physiology 300, no. 3 (2011): C588—C599. http://dx.doi.org/10.1152/ajpcell.00054.2010.

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Copper is an essential micronutrient in humans and is required for a wide range of physiological processes, including neurotransmitter biosynthesis, oxidative metabolism, protection against reactive oxygen species, and angiogenesis. The first step in the acquisition of dietary copper is absorption from the intestinal lumen. The major human high-affinity copper uptake protein, human copper transporter hCTR1, was recently shown to be at the basolateral or blood side of both intestinal and renal epithelial cell lines and thus does not play a direct role in this initial step. We sought to function

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14

Marvin, Marcus E., Robert P. Mason, and Annette M. Cashmore. "The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1." Microbiology 150, no. 7 (2004): 2197–208. http://dx.doi.org/10.1099/mic.0.27004-0.

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The ability of Candida albicans to acquire iron from the hostile environment of the host is known to be necessary for virulence and appears to be achieved using a similar system to that described for Saccharomyces cerevisiae. In S. cerevisiae, high-affinity iron uptake is dependent upon the acquisition of copper. The authors have previously identified a C. albicans gene (CaCTR1) that encodes a copper transporter. Deletion of this gene results in a mutant strain that grows predominantly as pseudohyphae and displays aberrant morphology in low-copper conditions. This paper demonstrates that invas

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15

Arredondo, Miguel, Patricia Muñoz, Casilda V. Mura, and Marco T. Núñez. "DMT1, a physiologically relevant apical Cu1+transporter of intestinal cells." American Journal of Physiology-Cell Physiology 284, no. 6 (2003): C1525—C1530. http://dx.doi.org/10.1152/ajpcell.00480.2002.

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Despite important advances in the understanding of copper secretion and excretion, the molecular components of intestinal copper absorption remain a mystery. DMT1, also known as Nramp2 and DCT1, is the transporter responsible for intestinal iron uptake. Electrophysiological evidence suggests that DMT1 can also be a copper transporter. Thus we examined the potential role of DMT1 as a copper transporter in intestinal Caco-2 cells. Treatment of cells with a DMT1 antisense oligonucleotide resulted in 80 and 48% inhibition of iron and copper uptake, respectively. Cells incorporated considerable amo

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16

Xie, Ruzhen, Yan Jin, Yao Chen, and Wenju Jiang. "The importance of surface functional groups in the adsorption of copper onto walnut shell derived activated carbon." Water Science and Technology 76, no. 11 (2017): 3022–34. http://dx.doi.org/10.2166/wst.2017.471.

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Abstract In this study, activated carbon (AC) was prepared from walnut shell using chemical activation. The surface chemistry of the prepared AC was modified by introducing or blocking certain functional groups, and the role of the different functional groups involved in the copper uptake was investigated. The structural and chemical heterogeneity of the produced carbons are characterized by Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, Boehm titration method and N2/77 K adsorption isotherm analysis. The equilibrium and the kinetics of copper adsorption onto AC wer

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17

Greipsson, S. "Effects of P on Growth and Uptake of Cu and Fe in Rice Grown in Excess Cu." International Rice Research Newsletter 17, no. 2 (1992): 19. https://doi.org/10.5281/zenodo.7218761.

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This article 'Effects of P on Growth and Uptake of Cu and Fe in Rice Grown in Excess Cu' appeared in the International Rice Research Newsletter series, created by the International Rice Research Institute (IRRI). The primary objective of this publication was to expedite communication among scientists concerned with the development of improved technology for rice and for rice based cropping systems. This publication will report what scientists are doing to increase the production of rice in as much as this crop feeds the most densely populated and land scarce nations in the world.

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18

Han, Okhee, and Marianne Wessling-Resnick. "Copper repletion enhances apical iron uptake and transepithelial iron transport by Caco-2 cells." American Journal of Physiology-Gastrointestinal and Liver Physiology 282, no. 3 (2002): G527—G533. http://dx.doi.org/10.1152/ajpgi.00414.2001.

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The influence of copper status on Caco-2 cell apical iron uptake and transepithelial transport was examined. Cells grown for 7–8 days in media supplemented with 1 μM CuCl2had 10-fold higher cellular levels of copper compared with control. Copper supplementation did not affect the integrity of differentiated Caco-2 cell monolayers grown on microporous membranes. Copper-repleted cells displayed increased uptake of iron as well as increased transport of iron across the cell monolayer. Northern blot analysis revealed that expression of the apical iron transporter divalent metal transporter-1 (DMT1

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19

Borgmann, U., and W. P. Norwood. "Kinetics of excess (above background) copper and zinc in Hyalella azteca and their relationship to chronic toxicity." Canadian Journal of Fisheries and Aquatic Sciences 52, no. 4 (1995): 864–74. http://dx.doi.org/10.1139/f95-086.

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One-week-long metal uptake experiments with Hyalella azteca are potentially a powerful tool for rapidly assessing the impact of copper or zinc in contaminated water samples. Copper and zinc concentrations in whole body Hyalella are independent of body size for both control and metal-exposed amphipods. Uptake rates are rapid for both metals, but copper concentrations in Hyalella during continuous exposure peak at about 1 week, then gradually decline back towards control levels at an apparent rate of 0.039 d−1. Hyalella can, therefore, control body copper concentrations during exposure to elevat

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20

Tabbì, Giovanni, Lorena Maria Cucci, Calogero Pinzino, et al. "Peptides Derived from Angiogenin Regulate Cellular Copper Uptake." International Journal of Molecular Sciences 22, no. 17 (2021): 9530. http://dx.doi.org/10.3390/ijms22179530.

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The angiogenin protein (ANG) is one of the most potent endogenous angiogenic factors. In this work we characterized by means of potentiometric, spectroscopic and voltammetric techniques, the copper complex species formed with peptide fragments derived from the N-terminal domain of the protein, encompassing the sequence 1-17 and having free amino, Ang1-17, or acetylated N-terminus group, AcAng1-17, so to explore the role of amino group in metal binding and cellular copper uptake. The obtained data show that amino group is the main copper anchoring site for Ang1-17. The affinity constant values,

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21

Wu, Guangxue, and Michael Rodgers. "Inhibitory effect of copper on enhanced biological phosphorus removal." Water Science and Technology 62, no. 7 (2010): 1464–70. http://dx.doi.org/10.2166/wst.2010.431.

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Copper inhibition of enhanced biological phosphorus removal (EBPR) was examined in batch experiments under anaerobic and aerobic conditions. Inhibition was represented by both acetate uptake and phosphorus release coefficients under anaerobic conditions, and by a phosphorus uptake coefficient under aerobic conditions. The results showed that copper inhibition of EBPR occurred mainly during aerobic phosphorus uptake and a first-order phosphorus uptake coefficient can be better used to describe the inhibition effect. For the aerobic phosphorus uptake using the EBPR activated sludge, (i) copper i

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22

Al Tarawneh, Amjad, Haitham Qaralleh, Muhamad Al-limoun, and Khaled Khleifat. "Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake." Journal of Basic and Applied Research in Biomedicine 5, no. 2 (2019): 107–14. http://dx.doi.org/10.51152/jbarbiomed.v5i2.42.

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The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeruginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive Bacillus thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained by the Pseudomonas aeruginosa. The metal uptake was dependent on the type of biosorbent with different accumulation affinities toward the tested chemical form of the copper. Cells harvested at exponential growth phase showed slightly higher Cu uptake

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23

Probst, Corinna, Sarela Garcia-Santamarina, Jacob T. Brooks, et al. "Interactions between copper homeostasis and the fungal cell wall affect copper stress resistance." PLOS Pathogens 18, no. 6 (2022): e1010195. http://dx.doi.org/10.1371/journal.ppat.1010195.

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Copper homeostasis mechanisms are essential for microbial adaption to changing copper levels within the host during infection. In the opportunistic fungal pathogen Cryptococcus neoformans (Cn), the Cn Cbi1/Bim1 protein is a newly identified copper binding and release protein that is highly induced during copper limitation. Recent studies demonstrated that Cbi1 functions in copper uptake through the Ctr1 copper transporter during copper limitation. However, the mechanism of Cbi1 action is unknown. The fungal cell wall is a dynamic structure primarily composed of carbohydrate polymers, such as c

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24

Horgan, D. B., and R. E. Gaskin. "The effect of copper on the uptake and translocation of spirotetramat insecticide on kiwifruit." New Zealand Plant Protection 68 (January 8, 2015): 26–31. http://dx.doi.org/10.30843/nzpp.2015.68.5794.

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Spirotetramat (Movento) is a systemic insecticide that is used to control scale insects on kiwifruit The use of protectant copper sprays on kiwifruit has become increasingly common due to the bacterial disease Pseudomonas syringae pv actinidiae This study investigated the interaction of copper with spirotetramat and how it influenced the uptake and translocation of spirotetramat within the plant Movento 100SC sprays should not be tank with copper sprays because the uptake and translocation of spirotetramat is likely to be compromised These negative effects were minimised when an organosilicone

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25

Shinada, Mitsuhiro, Masashi Takahashi, Chika Igarashi, et al. "64Cu2+ Complexes of Tripodal Amine Ligands’ In Vivo Tumor and Liver Uptakes and Intracellular Cu Distribution in the Extrahepatic Bile Duct Carcinoma Cell Line TFK-1: A Basic Comparative Study." Pharmaceuticals 17, no. 7 (2024): 820. http://dx.doi.org/10.3390/ph17070820.

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Copper (Cu) is a critical element for cancer cell proliferation and considerably accumulates in the nucleus. 64Cu2+ is an anticancer radiopharmaceutical that targets the copper requirement of cancer cells. However, intravenously injected 64Cu2+ ions primarily accumulate in the liver. Ligand complexation of 64Cu2+ may be a promising method for increasing tumor delivery by reducing liver uptake. In this study, we used three tripodal amine ligands [tris(2-aminoethyl)amine (Tren), diethylenetriamine (Dien), and tris(2-pyridylmethyl)amine (TPMA)] to enclose 64Cu2+ ions and compared their in vivo tu

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26

Kenney, Grace E., and Amy C. Rosenzweig. "Chalkophores." Annual Review of Biochemistry 87, no. 1 (2018): 645–76. http://dx.doi.org/10.1146/annurev-biochem-062917-012300.

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Copper-binding metallophores, or chalkophores, play a role in microbial copper homeostasis that is analogous to that of siderophores in iron homeostasis. The best-studied chalkophores are members of the methanobactin (Mbn) family—ribosomally produced, posttranslationally modified natural products first identified as copper chelators responsible for copper uptake in methane-oxidizing bacteria. To date, Mbns have been characterized exclusively in those species, but there is genomic evidence for their production in a much wider range of bacteria. This review addresses the current state of knowled

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27

Civardi, Chiara, Daniel Grolimund, Mark Schubert, Peter Wick, and Francis W. M. R. Schwarze. "Micronized copper-treated wood: copper remobilization into spores from the copper-tolerant wood-destroying fungus Rhodonia placenta." Environmental Science: Nano 6, no. 2 (2019): 425–31. http://dx.doi.org/10.1039/c8en01110a.

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28

Fukushi, K., S. Babel, and H. Xuan. "Isolation and examination of copper removing bacteria from activated sludge culture." Water Science and Technology 46, no. 11-12 (2002): 189–94. http://dx.doi.org/10.2166/wst.2002.0737.

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Experiments were carried out to isolate metal removing bacteria from activated sludge culture and test the metal removal capability of isolated bacteria. Two concentrations of copper solution (CuCl2) used to select the copper-tolerance level of each isolate were 0.05 mM and 2.5 mM. Experimental data showed that isolates could be obtained by using non-selective medium (NSM) in the presence of copper. The isolates were displayed on agar plates with diverse colonial morphological characteristics. A maximum uptake of 81 mg/g dry cell mass was observed at an initial copper concentration of 50 mg/l.

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29

Lee, Terrence A., and James K. Hardy. "Copper uptake by the water hyacinth." Journal of Environmental Science and Health . Part A: Environmental Science and Engineering 22, no. 2 (1987): 141–60. http://dx.doi.org/10.1080/10934528709375340.

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30

Sheffield, Ann, and Margaret J. Doyle. "Uptake of Copper(II) by Wool." Textile Research Journal 75, no. 3 (2005): 203–7. http://dx.doi.org/10.1177/004051750507500303.

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Quartacci, Mike F., Elena Cosi, Sandra Meneguzzo, Cristina Sgherri, and Flavia Navari‐Izzo. "Uptake and Translocation of Copper inBrassicaceae." Journal of Plant Nutrition 26, no. 5 (2003): 1065–83. http://dx.doi.org/10.1081/pln-120020076.

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32

Cooksey, Donald A. "Copper uptake and resistance in bacteria." Molecular Microbiology 7, no. 1 (1993): 1–5. http://dx.doi.org/10.1111/j.1365-2958.1993.tb01091.x.

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33

Trevors, J. T., and C. M. Cotter. "Copper toxicity and uptake in microorganisms." Journal of Industrial Microbiology 6, no. 2 (1990): 77–84. http://dx.doi.org/10.1007/bf01576426.

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34

Ross, I. S., and M. J. Parkin. "Uptake of copper by Candida utilis." Mycological Research 93, no. 1 (1989): 33–37. http://dx.doi.org/10.1016/s0953-7562(89)80132-7.

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35

Lüderitz, V., and F. Scholz. "Copper Uptake by Blue-Green Algae." Acta Hydrochimica et Hydrobiologica 17, no. 5 (1989): 537–42. http://dx.doi.org/10.1002/aheh.19890170509.

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36

McArdle, Harry J., Sharon M. Gross, and David M. Danks. "Uptake of copper by mouse hepatocytes." Journal of Cellular Physiology 136, no. 2 (1988): 373–78. http://dx.doi.org/10.1002/jcp.1041360223.

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37

Li, Jinjin, Jinhong Yuan, Hui Wang, Hui Zhang, and Haiyan Zhang. "Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner." Journal of Experimental Botany 71, no. 19 (2020): 6174–86. http://dx.doi.org/10.1093/jxb/eraa352.

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Abstract The essential nutrient copper is toxic in excess. Therefore, plants must tightly control copper uptake and distribution. Arabidopsis thaliana high-affinity copper transporters (COPTs) mediate copper uptake, partitioning, and redistribution. Here we show that COPT1 localizes to the plasma membrane and endoplasmic reticulum in stably transgenic plants expressing a COPT1–green fluorescent protein (GFP) fusion protein, and the fusion protein is rapidly degraded upon plant exposure to excess copper. MG132 treatment largely abolished copper-induced degradation of COPT1, implying a link betw

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38

Marvin, Marcus E., Peter H. Williams, and Annette M. Cashmore. "The Candida albicans CTR1 gene encodes a functional copper transporter." Microbiology 149, no. 6 (2003): 1461–74. http://dx.doi.org/10.1099/mic.0.26172-0.

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Copper and iron uptake in Saccharomyces cerevisiae are linked through a high-affinity ferric/cupric-reductive uptake system. Evidence suggests that a similar system operates in Candida albicans. The authors have identified a C. albicans gene that is able to rescue a S. cerevisiae ctr1/ctr3-null mutant defective in high-affinity copper uptake. The 756 bp ORF, designated CaCTR1, encodes a 251 amino acid protein with a molecular mass of 27·8 kDa. Comparisons between the deduced amino acid sequence of the C. albicans Ctr1p and S. cerevisiae Ctr1p indicated that they share 39·6 % similarity and 33·

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39

Peña, Maria Marjorette O., Keith A. Koch, and Dennis J. Thiele. "Dynamic Regulation of Copper Uptake and Detoxification Genes in Saccharomyces cerevisiae." Molecular and Cellular Biology 18, no. 5 (1998): 2514–23. http://dx.doi.org/10.1128/mcb.18.5.2514.

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ABSTRACT The essential yet toxic nature of copper demands tight regulation of the copper homeostatic machinery to ensure that sufficient copper is present in the cell to drive essential biochemical processes yet prevent the accumulation to toxic levels. In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genesCTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, andSOD1 in response to copper. In this study, we characterize

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40

Waldrop, G. L., F. A. Palida, M. Hadi, P. A. Lonergan, and M. J. Ettinger. "Effect of albumin on net copper accumulation by fibroblasts and hepatocytes." American Journal of Physiology-Gastrointestinal and Liver Physiology 259, no. 2 (1990): G219—G225. http://dx.doi.org/10.1152/ajpgi.1990.259.2.g219.

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The liver accumulates copper rapidly and preferentially from plasma. The effects of albumin on net copper accumulation by fibroblasts and hepatocytes were compared to determine whether preferential uptake involves hepatocyte-specific sequestering of copper. Although albumin inhibits the initial rates (30 s) of copper transport by fibroblasts and hepatocytes similarly, the effects of albumin on net copper accumulation (4 h) by these cell types were strikingly different. Fibroblasts accumulate only approximately 15% as much copper when equimolar albumin is present as from albumin-free media; hep

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41

Laurén, Darrel Jon, and D. G. McDonald. "Influence of Water Hardness, pH, and Alkalinity on the Mechanisms of Copper Toxicity in Juvenile Rainbow Trout, Salmo gairdneri." Canadian Journal of Fisheries and Aquatic Sciences 43, no. 8 (1986): 1488–96. http://dx.doi.org/10.1139/f86-186.

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Juvenile rainbow trout were exposed to 25–400 μg copper∙L−1 for 24 h. Water hardness, pH, and alkalinity were varied independently at a constant [Na+]. Net and unidirectional sodium fluxes were measured in hard and soft, low-alkalinity water and in hard, high-alkalinity water at neutral pH and pH 5.0. In low alkalinity water, Na+ uptake (Jin) was inhibited at copper concentrations as low as 25 μg∙L−1, and sodium efflux (Jout) was stimulated above 100 μg∙L−1. High-alkalinity water significantly reduced the effects of copper on Jin and Jout, but there was no significant effect of increasing wate

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42

Anderson, Lars W. J., Nathan Dechoretz, David Bayer, and Gary L. Darmstadt. "Effect of Three Formulations on Uptake and Efficacy of Copper inHydrilla verticillata." Weed Science 35, no. 2 (1987): 263–69. http://dx.doi.org/10.1017/s0043174500079170.

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Copper content and growth of excised hydrilla [DioeciousHydrilla verticillata(L.f.) Royle # HYLLI] apical shoot segments were determined following exposure to copper sulfate (CuSO4), copper-triethanolamine (Cu-TEA), and copper-ethylenediamine (Cu-EDA). For all copper formulations, inhibition of growth was related to the amount of copper associated with the excised shoots. At equal copper exposure, the Cu-EDA formulation produced the greatest inhibition of growth and generally the highest copper levels in the plants. The Cu-EDA formulation inhibited dry weight gain by more than 80% 3 weeks afte

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43

Yildiz, Sibel, Sabriye Canakci, Umit C. Yildiz, Ozlem Ozgenc, and Eylem D. Tomak. "Improving of the impregnability of refractory spruce wood by Bacillus licheniformis pretreatment." BioResources 7, no. 1 (2011): 565–77. http://dx.doi.org/10.15376/biores.7.1.565-577.

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In this study it was aimed to improve impregnability of spruce (Picea orientalis L.) wood with bacteria (Bacillus licheniformis A1) pretreatment, using copper/chromium/arsenic Type C (CCA-C) andcopper azole Type A (CBA-A). The effects of Bacillus licheniformis A1 on weight loss, copper uptake, and compression strength of samples were determined. Weight loss was slightly changed by bacterial degradation in all test groups. The best copper uptake cases were 1466 ppm for CCA-C and 2730 ppm for CBA-A. Improvement on copper uptake with bacteria pretreatment was in a range of 18 to 103% compared to

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44

McArdle, H. J., S. M. Gross, D. M. Danks, and A. G. Wedd. "Role of albumin's copper binding site in copper uptake by mouse hepatocytes." American Journal of Physiology-Gastrointestinal and Liver Physiology 258, no. 6 (1990): G988—G991. http://dx.doi.org/10.1152/ajpgi.1990.258.6.g988.

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It is possible, in vitro, to label albumin with copper either exclusively on the specific binding site or partly on the specific site and also on other sites by altering the pH at which the two ligands are mixed. Copper attached exclusively to the specific site is taken up more rapidly than copper attached to that site and others on albumin. The effect is proportional to the amount of copper on the specific site. Additional histidine stimulates uptake irrespective of the copper binding site on albumin. The effect is related to the histidine on position 3 of the albumin, since it is not seen wh

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Kim, Heejeong, Hwa-Young Son, Sarah M. Bailey, and Jaekwon Lee. "Deletion of hepatic Ctr1 reveals its function in copper acquisition and compensatory mechanisms for copper homeostasis." American Journal of Physiology-Gastrointestinal and Liver Physiology 296, no. 2 (2009): G356—G364. http://dx.doi.org/10.1152/ajpgi.90632.2008.

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Copper is a vital trace element required for normal growth and development of many organisms. To determine the roles for copper transporter 1 (Ctr1) in hepatic copper metabolism and the contribution of the liver to systemic copper homeostasis, we have generated and characterized mice in which Ctr1 is deleted specifically in the liver. These mice express less than 10% residual Ctr1 protein in the liver and exhibit a small but significant growth retardation, which disappears with age. Hepatic copper concentrations and the activities of copper-requiring enzymes are reduced; however, mild copper d

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46

Yläranta, Toivo. "Uptake of heavy metals by plants from airborne deposition and polluted soils." Agricultural and Food Science 5, no. 4 (1996): 431–47. http://dx.doi.org/10.23986/afsci.72755.

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The concentrations of sulphur, zinc, copper, lead and cadmium in spring wheat grain and straw, Italian rye grass, timothy and lettuce were studied in a three-year field experiment conducted in southern Finland near a copper-nickel smelter and at nonpolluted control sites. A pot experiment with copper- and nickel-contaminated soils and with a nonpolluted soil as the control was conducted to determine the copper and nickel concentrations in soils phytotoxic for plants. Forty, 200 or 1000 mg of copper or nickel as cloride was added to 2 litres of soil. The nickel and copper concentrations in the

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47

Sung Lee, Hak, and Bohumil Volesky. "Interference of Aluminum in Copper Biosorption by an Algal Biosorbent." Water Quality Research Journal 34, no. 3 (1999): 519–32. http://dx.doi.org/10.2166/wqrj.1999.025.

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Abstract Cu and Al sorption capacities of NaOH-treated Sargassum fluitans biomass were studied using equilibrium methodology. An evaluation of sorption performance and modeling in a two-metal system was carried out using a modified multicomponent Langmuir isotherm. The maximum Cu and Al uptakes calculated from the Langmuir isotherm were 1.54 mmol/g (9.8 weight percent) and 3.75 mmol/g (10.1 weight percent) at pH 4.5, respectively. However, these values were 1.35 mmol/g for Cu and 1.58 mmol/g for Al at pH 3.5. The modified Langmuir model gave the following affinity correlated coefficients: 0.20

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Alda, J. O., and R. Garay. "Chloride (or bicarbonate)-dependent copper uptake through the anion exchanger in human red blood cells." American Journal of Physiology-Cell Physiology 259, no. 4 (1990): C570—C576. http://dx.doi.org/10.1152/ajpcell.1990.259.4.c570.

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The initial rate of Cu2+ uptake in human red blood cells was measured by atomic absorption. About 80% of Cu2+ uptake was inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) concentrations greater than 5-10 microM. DIDS-sensitive Cu2+ uptake required the presence of external HCO3- or external Cl-. Cl- strongly stimulated Cu2+ uptake following a Michaelis-like function, with apparent dissociation constant (KCl) of 72 +/- 9.4 (SD) mM (n = 6 experiments). HCO3- stimulated DIDS-sensitive Cu2+ uptake following a Michaelis-like function, with apparent dissociation constant (Kbic) of

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49

Magrì, Antonio, Diego La Mendola, and Enrico Rizzarelli. "Nerve Growth Factor Peptides Bind Copper(II) with High Affinity: A Thermodynamic Approach to Unveil Overlooked Neurotrophin Roles." International Journal of Molecular Sciences 22, no. 10 (2021): 5085. http://dx.doi.org/10.3390/ijms22105085.

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Nerve growth factor (NGF) is a protein essential to neurons survival, which interacts with its receptor as a non-covalent dimer. Peptides belonging to NGF N-terminal domain are able to mimic the activity of the whole protein. Such activity is affected by the presence of copper ions. The metal is released in the synaptic cleft where proteins, not yet identified, may bind and transfer to human copper transporter 1 (hCtr1), for copper uptake in neurons. The measurements of the stability constants of copper complexes formed by amyloid beta and hCtr1 peptide fragments suggest that beta-amyloid (Aβ)

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50

Bingham, M. J., T. J. Ong, W. J. Ingledew, and H. J. McArdle. "ATP-dependent copper transporter, in the Golgi apparatus of rat hepatocytes, transports Cu(II) not Cu(I)." American Journal of Physiology-Gastrointestinal and Liver Physiology 271, no. 5 (1996): G741—G746. http://dx.doi.org/10.1152/ajpgi.1996.271.5.g741.

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The Wilson disease adenosinetriphosphatase (ATPase; ATP7B) is believed to bind copper as Cu(I). We provide evidence to suggest that the ATPase actually transports Cu as Cu(II). When the copper is presented to rat liver microsomes as Cu(I), virtually all uptake is ATP independent. If the copper is presented as copper oxalate [Cu(II)], total uptake is reduced to approximately 10% of Cu(I) levels, but ATP-dependent uptake rises, both as a proportion of total uptake and in absolute terms. The reducing agent vitamin C and the Cu(I) chelator bathocuproine both override the effect of oxalate. The dat

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