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Journal articles on the topic 'Corn anthracnose'
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1
Jirak-Peterson, Jennifer C., and Paul D. Esker. "Tillage, Crop Rotation, and Hybrid Effects on Residue and Corn Anthracnose Occurrence in Wisconsin." Plant Disease 95, no. 5 (2011): 601–10. http://dx.doi.org/10.1094/pdis-11-10-0837.
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Corn anthracnose (Colletotrichum graminicola) is an important disease of field corn (Zea mays). Two phases, leaf blight and stalk rot, can reduce yield through either premature leaf senescence or reduced grain harvest due to stalk lodging. Corn residue is an important source of primary inoculum and is increased through cultural practices such as no-tillage and continuous corn cropping, which are common practices in Wisconsin. Field studies conducted at the Arlington Agricultural Research Station (ARS) and the West Madison ARS showed that the incidence and severity of anthracnose leaf blight were higher in continuous-corn crop rotations than in soybean–corn rotations (91% higher incidence, 24 to 78% higher severity). Anthracnose stalk rot was marginally affected by tillage in 2008 (P = 0.09), with higher incidence in chisel-plowed treatments. There was a positive association between spring residue cover and anthracnose leaf blight but no association was found between residue and stalk rot. No association was found between anthracnose leaf blight and stalk rot. There was a negative association between anthracnose leaf blight and yield but not between anthracnose stalk rot and yield. Managing residue levels through crop rotation would help to reduce anthracnose leaf blight but further work is needed to elucidate factors that lead to stalk lodging prior to harvest.
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Lipps, Patrick E. "Spread of Corn Anthracnose from Surface Residues in Continuous Corn and Corn-Soybean Rotation Plots." Phytopathology 78, no. 6 (1988): 756. http://dx.doi.org/10.1094/phyto-78-756.
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Toman, Jr.,, J. "Inheritance of Resistance to Anthracnose Stalk Rot of Corn." Phytopathology 83, no. 7 (1993): 981. http://dx.doi.org/10.1094/phyto-83-981.
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Reid, L. M., X. Zhu, C. Voloaca, et al. "CO450 corn inbred line." Canadian Journal of Plant Science 94, no. 1 (2014): 161–67. http://dx.doi.org/10.4141/cjps2013-190.
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Reid, L. M., Zhu, X., Voloaca, C., Wu, J., Woldemariam, T., Martin, R. A. and Beres, B. L. 2014. CO450 corn inbred line. Can. J. Plant Sci. 94: 161–167. CO450 is a short-season early-maturing (average 71 d to flowering) corn (Zea mays L.) inbred line with excellent combining ability and superior resistance to eyespot (Aureobasidium zeae). This is the first inbred to be released from AAFC's eyespot resistance breeding program. CO450 also has moderate resistance to common rust, intermediate resistance to common smut and both fusarium and anthracnose stalk rot. Excellent hybrid yields and performance data were achieved when CO450 was crossed with stiff stalk inbreds such as MBS1130 and an Iodent inbred, MBS8148. CO450 also exhibited good performance at sites with 2400 CHU or less in combination with a very early inbred, CL30.
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Lipps, P. E. "Influence of Inoculum From Buried and Surface Corn Residues on The Incidence of Corn Anthracnose." Phytopathology 75, no. 11 (1985): 1212. http://dx.doi.org/10.1094/phyto-75-1212.
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Bergstrom, Gary C., and Ralph L. Nicholson. "The Biology of Corn Anthracnose: Knowledge to Exploit for Improved Management." Plant Disease 83, no. 7 (1999): 596–608. http://dx.doi.org/10.1094/pdis.1999.83.7.596.
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Adee, Eric, and Stewart Duncan. "Timing of Strobilurin Fungicide for Control of Top Dieback in Corn." Plant Health Progress 18, no. 2 (2017): 129–35. http://dx.doi.org/10.1094/php-03-17-0020-rs.
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Significant yield losses can result from top dieback (TDB) in dent corn (Zea mays L.), which is caused by infection by the fungus Colletotrichum graminicola, which also causes anthracnose. Research on the effectiveness of fungicide application is limited because of the unpredictable nature of the disease. Three field studies were established to assess the timing of fungicide application for foliar diseases that developed TDB, one in Illinois (2010) and the other two in Kansas (2015 and 2016). Fungicide applications at tasseling and later were effective in reducing the incidence of TDB by over 20% and increasing yield over 900 kg/ha, or over 7%, while earlier applications (V5 to V8) did not reduce TDB nor increase yield compared with the untreated check.
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Cota, Luciano V., Rodrigo V. da Costa, Dagma D. Silva, Carlos R. Casela, and Douglas F. Parreira. "Quantification of Yield Losses Due to Anthracnose Stalk Rot on Corn in Brazilian Conditions." Journal of Phytopathology 160, no. 11-12 (2012): 680–84. http://dx.doi.org/10.1111/jph.12008.
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Lyons, Philip C., and Ralph L. Nicholson. "Evidence that cyclic hydroxamate concentrations are not related to resistance of corn leaves to anthracnose." Canadian Journal of Plant Pathology 11, no. 3 (1989): 215–20. http://dx.doi.org/10.1080/07060668909501102.
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Jauhari, Chafif, and Abdul Majid. "KAJIAN JENIS FUNGISIDA DAN INTERVAL APLIKASI TERHADAP PERKEMBANGAN PENYAKIT ANTRAKNOSA PADA KEDELAI." JURNAL BIOINDUSTRI 2, no. 1 (2019): 307–18. http://dx.doi.org/10.31326/jbio.v2i1.477.
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Soybean is an important commodity after rice and corn. The attack of plant pest organisms in cultivation decreased soybean productivity. Anthracnose is a disease that can reduce soybean production to 95%. The use of excessive synthetic chemical fungicides causes resistance to plant disturbing organisms and leaves chemical residues. The solution to overcome this problem is pay attention using types of fungicide active ingredients and span application time. This study aims to determine the effect of several types of fungicides and span application time on the development of anthracnose and yields. This research was carried out from February to April 2018 on Agroteknopark Jubung land of Jember University. This study used a Factorial Randomized Group Design model with 9 combinations of treatments and 4 replications. The first factor of the type of fungicide consisted of three levels, namely Triadimefon, T. harzianum and betel leaf. The second factor is the span application time of 3.5 and 7 days. The research data were analyzed using variance analysis, if there were significant differences Duncan's Multiple Range Test (DMRT) test was carried out at the level of 5%. Observation parameters included the disease incidence, diseases severity, rate of infection, and weight of pods. The results of the study showed that the combination treatment of PT3 (vegetable fungicide with betel leaves with span application time of 7 days) was the most efficient than the other treatments and gave the results of the rate of disease infection (0.84 units per day).Keywords: anthracnose, fungicide, infection rate, soybeans
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Crouch, Jo Anne, Bruce B. Clarke, and Bradley I. Hillman. "Unraveling Evolutionary Relationships Among the Divergent Lineages of Colletotrichum Causing Anthracnose Disease in Turfgrass and Corn." Phytopathology® 96, no. 1 (2006): 46–60. http://dx.doi.org/10.1094/phyto-96-0046.
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Colletotrichum species cause anthracnose diseases on a number of grass hosts and are common inhabitants of many others. They are divided into four species: C. sublineolum is pathogenic to Sorghum spp.; C. caudatum is found on C4 grasses such as indiangrass and big bluestem; C. falcatum causes red rot of sugarcane; and C. graminicola sensu lato is a broadly defined species including isolates that attack maize, wheat, oats, and many forage, turf, and amenity grasses of the subfamily Pooideae. In this paper, a combination of hierarchal- and nonhierarchal-based analyses were employed to examine evolutionary relationships among the grass-infecting Colletotrichum species, with special emphasis on isolates from turf and other grasses in the subfamily Pooideae. Reconstructions performed with data sets from over 100 Colletotrichum isolates at three variable loci using phylogenetic and network-based methodologies unambiguously supported the taxonomic separation of maize-infecting isolates of C. graminicola from the pooid-infecting strains of Colletotrichum. To reflect the evolutionary relationships that exist between these distinct lineages, we propose the resurrection of the species name C. cereale to describe the pooid-infecting isolates. There was also support for further subdivision of C. cereale, but the current data are insufficient to confidently subdivide the species, as there was some evidence of recombination between lineages of this species.
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Keller, N. P. "Potential Yield Reductions in Maize Associated with an Anthracnose/European Corn Borer Pest Complex in New York." Phytopathology 76, no. 6 (1986): 586. http://dx.doi.org/10.1094/phyto-76-586.
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Nicholson, R. L. "Single and Combined Effects of the Lesion Nematode andColletotrichum graminicolaon Growth and Anthracnose Leaf Blight of Corn." Phytopathology 75, no. 6 (1985): 654. http://dx.doi.org/10.1094/phyto-75-654.
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Rehman, Fazal Ur, Muhammad Adnan, Maria Kalsoom, et al. "Seed-Borne Fungal Diseases of Maize (Zea mays L.): A Review." Agrinula : Jurnal Agroteknologi dan Perkebunan 4, no. 1 (2021): 43–60. http://dx.doi.org/10.36490/agri.v4i1.123.
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 Introduction: Maize (Zea mays) is one of the most important cereal crops. It is ranked as 3rd after wheat and rice. Due to its wide adaptability, diversified uses, and low production costs, it has great potential as a cereal crop. In the case of yield losses, various factors are involved. The fungal diseases of maize play a significant role in the reduction of both quantity as well as the quality of maize.
 Review Results: At the seedling stage, maize suffers from numerous diseases and many of them are seed-borne diseases. Anthracnose stalk rot (Colletotrichum graminicola), Charcoal rot of maize (Macrophomina phaseolina), Crazy top downy mildew disease (Sclerophthora macrospora), Corn grey leaf spot disease (Cercospora zeae-maydis), Aspergillus ear and kernel rot (Aspergillus flavus), Corn smut (Ustilago maydis), Southern corn leaf blight disease (Bipolaris maydis) etc. are important among these diseases.Chemical control of seed-borne pathogens of maize is rather difficult to achieve as a reasonably good. Due to the hazardous environmental effects of chemicals, the Integrated Management of the seed-borne fungal pathogens of corn is mostly preferred. The distribution, disease cycle, symptoms of the damage, effects of environmental factors, economical importance of disease, and integrated disease management options of major seed-borne fungal pathogens of maize have been reviewed in this review article from various currently available sources.
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Nyhus, K. A. "Reaction of Two Maize Synthetics to Anthracnose Stalk Rot and Northern Corn Leaf Blight Following Recurrent Selection for Resistance to Diplodia Stalk Rot and European Corn Borer." Phytopathology 79, no. 2 (1989): 166. http://dx.doi.org/10.1094/phyto-79-166.
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Krijger, Jorrit-Jan, Ralf Horbach, Michael Behr, Patrick Schweizer, Holger B. Deising, and Stefan G. R. Wirsel. "The Yeast Signal Sequence Trap Identifies Secreted Proteins of the Hemibiotrophic Corn Pathogen Colletotrichum graminicola." Molecular Plant-Microbe Interactions® 21, no. 10 (2008): 1325–36. http://dx.doi.org/10.1094/mpmi-21-10-1325.
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The hemibiotroph Colletotrichum graminicola is the causal agent of stem rot and leaf anthracnose on Zea mays. Following penetration of epidermal cells, the fungus enters a short biotrophic phase, followed by a destructive necrotrophic phase of pathogenesis. During both phases, secreted fungal proteins are supposed to determine progress and success of the infection. To identify genes encoding such proteins, we constructed a yeast signal sequence trap (YSST) cDNA-library from RNA extracted from mycelium grown in vitro on corn cell walls and leaf extract. Of the 103 identified unigenes, 50 showed significant similarities to genes with a reported function, 25 sequences were similar to genes without a known function, and 28 sequences showed no similarity to entries in the databases. Macroarray hybridization and quantitative reverse-transcriptase polymerase chain reaction confirmed that most genes identified by the YSST screen are expressed in planta. Other than some genes that were constantly expressed, a larger set showed peaks of transcript abundances at specific phases of pathogenesis. Another set exhibited biphasic expression with peaks at the biotrophic and necrotrophic phase. Transcript analyses of in vitro-grown cultures revealed that several of the genes identified by the YSST screen were induced by the addition of corn leaf components, indicating that host-derived factors may have mimicked the host milieu.
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Boyette, C. Douglas, and Robert E. Hoagland. "Biological Control of Hemp sesbania (Sesbania exaltata) and Sicklepod (Senna obtusifolia) in Soybean with Anthracnose Pathogen Mixtures." Weed Technology 24, no. 4 (2010): 551–56. http://dx.doi.org/10.1614/wt-08-154.1.
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In greenhouse and field experiments conducted over 3 yr, tank mixtures of spores of the fungiColletotrichum truncatumandColletotrichum gloeosporioides, formulated in unrefined corn oil and Silwet L-77 surfactant, were evaluated as a mycoherbicide mixture for simultaneous control of hemp sesbania and sicklepod, respectively. In greenhouse tests, 100% mortality and dry weight reduction of hemp sesbania occurred 6 d after treatment (DAT), whereas 15 d were required to achieve 100% mortality and dry weight reduction of sicklepod. In field experiments conducted in narrow-row (51-cm) soybean test plots, a single application of the fungal mixture formulated as described controlled hemp sesbania and sicklepod 94% and 88%, respectively, 28 DAT. Neither fungus, applied as aqueous conidial suspensions, provided control of their respective weed hosts. These results indicate that tank mixtures of these anthracnose-forming pathogens can effectively control hemp sesbania and sicklepod with a single application.
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Waxman, K. D., and G. C. Bergstrom. "First Report of Anthracnose Caused by Colletotrichum navitas on Switchgrass in New York." Plant Disease 95, no. 8 (2011): 1032. http://dx.doi.org/10.1094/pdis-03-11-0164.
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Switchgrass (Panicum virgatum L.) is a perennial grass with significant potential as a biofuel crop. From 2007 to 2010, foliar lesions were observed in new and mature stands of switchgrass in various locations in New York. Lesions were elliptical with purple margins and white necrotic centers, generally <3 cm long, ~1 mm wide, often coalesced, and containing black setae. Upon incubation, symptomatic leaf tissue developed acervuli with masses of salmon-colored spores. The fungus was identified as Colletotrichum nativas Crouch on the basis of typical cultural characteristics and conidial morphology (1). Conidia were one-celled, hyaline, fusiform, and generally falcate. Conidial length averaged 40 μm (22 to 47 μm) and width averaged 5 μm (4 to 7 μm). Compared with other graminicolous species of Colletotrichum, the conidia were larger and varied from straight to irregularly bent. Sequences of the rDNA internal transcribed spacer (ITS) regions of three isolates (Cornell accession and corresponding GenBank Nos.: Cn071NY08 (from a >20-year-old naturalized stand of switchgrass in Steuben County), JF437053; Cn080NY08 (from ‘Pathfinder’ in Chemung County), JF437054; and Cn101NY09 (from ‘Blackwell’ in Chemung County), JF437055) exhibited 100% nucleotide identity to the type isolate of C. nativas (GenBank No. GQ919068) collected from switchgrass selection ‘Brooklyn’ in New Jersey (1). Pathogenicity of the sequenced isolates along with seven other isolates (Cn105NY09 from ‘Sunburst’ in Tompkins County; Cn107NY09 from ‘Trailblazer’ in Tompkins County; Cn109NY09 from ‘Forestburg’ in Tompkins County; Cn111NY09 and Cn112NY09 from ‘Shelter’ in Tompkins County; and Cn122NY09 and Cn123NY09 from ‘Cave-in-Rock’ in Genesee County) was evaluated in greenhouse experiments. Seven- to eight-week-old switchgrass plants were inoculated with conidial suspensions (1 × 106 conidia/ml) of C. nativas. Inoculum or sterilized water was sprayed until runoff. Three plants of each of ‘Cave-in-Rock’ and ‘Kanlow’ were sprayed per treatment and the experiment was repeated for 3 of the 10 isolates. Inoculated plants were placed in a mist chamber for 48 h before they were returned to the greenhouse and observed for disease development, which occurred within 1 week of inoculation for both cultivars. No symptoms developed on the control plants. Foliar lesions closely resembled those observed in the field. C. nativas was consistently reisolated from symptomatic tissue collected from greenhouse experiments. Switchgrass anthracnose associated with C. graminicola sensu lata has been reported in many U.S. states (2). On the basis of molecular phylogenetics and distinguishing morphological characters, Crouch et al. erected C. navitas as a novel species distinct from C. graminicola sensu stricto, a taxon restricted to the corn anthracnose pathogen (1). C. nativas was first documented on switchgrass in New Jersey (1) and appears to be the same pathogen causing anthracnose of switchgrass in the adjoining state of Pennsylvania (1,3). To our knowledge, this is the first report of C. nativas causing anthracnose of switchgrass in New York. References: (1) J. A. Crouch et al. Mycol. Res. 113:1411, 2009. (2) D. F. Farr and A. Y. Rossman. Fungal Databases, Systematic Mycology and Microbiology Laboratory, ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ , May 5, 2011. (3) M. A. Sanderson et al. Agron. J. 100:510, 2008.
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Soltani, Nader, and George Lazarovits. "486 Growth Responses of Tomato, Pepper, Broccoli, and Corn Grown in Soils Amended with Ammonium Lignosulfonate." HortScience 34, no. 3 (1999): 528E—529. http://dx.doi.org/10.21273/hortsci.34.3.528e.
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Ammonium lignosulfonate (ALS) is a liquid waste by-product of pulp and paper industry that may be a source of organic fertilizer. Four plots each of tomato, pepper, broccoli, and corn were set up in a randomized block design on the AAFC-SCPFRC farm in the Spring 1998. Treatments were untreated control, 0.5% (v/w) ALS, and 1% (v/w) ALS. Soil samples were taken at 0, 2, 4, 8, and 22 weeks after amendment incorporation and analyzed for pH, microbial population, and water soluble ions. Soil temperature was measured at 8-cm depth. Leaf chlorophyll content was measured at four sampling dates. Tomato and pepper fruit were evaluated for symptoms of diseases. Soil temperature in 0.5% and 1.% ALS treatments were 2 and 7 °C warmer, respectively, than the control. Soil pH was lower in ALS-treated plots. 1% ALS caused more than 10-fold increase in bacterial population. Fungal populations in both 0.5% and 1% ALS treatments were 10- to 100-fold higher than control soil and continued to be higher to the last sampling date. Weeds were reduced by more than 50% by 0.5% or 1% ALS treatments. Both ALS rates caused an initial increase in NH4, NO3, NO2, K, Na, Cl, PO4, Ca, and SO4. NH4 and SO4 remained elevated for 22 weeks in both ALS treatments. ALS slightly increased chlorophyll content in tomato, pepper, and corn, but not in broccoli plants. The number of diseased tomato fruit in ALS plots were reduced by 50% to 70%. Bacterial spot decreased by more than 50% in both ALS-treated plots, while anthracnose declined by 50% to 75%. There were no significant differences in early and total yield of tomato, peppers, and corn. Early broccoli yield decreased in ALS treatments, while total yield increased over that of control in both ALS treatments.
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Ludwig, Nancy, Marco Löhrer, Marcus Hempel, et al. "Melanin Is Not Required for Turgor Generation but Enhances Cell-Wall Rigidity in Appressoria of the Corn Pathogen Colletotrichum graminicola." Molecular Plant-Microbe Interactions® 27, no. 4 (2014): 315–27. http://dx.doi.org/10.1094/mpmi-09-13-0267-r.
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The ascomycete and causative agent of maize anthracnose and stem rot, Colletotrichum graminicola, differentiates melanized infection cells called appressoria that are indispensable for breaching the plant cell wall. High concentrations of osmolytes accumulate within the appressorium, and the internal turgor pressure of up to 5.4 MPa provides sufficient force to penetrate the leaf epidermis directly. In order to assess the function of melanin in C. graminicola appressoria, we identified and characterized the polyketide synthase 1 (CgPKS1) gene which displayed high similarity to fungal polyketide synthases (PKS) involved in synthesis of 1,3,6,8-tetrahydronaphthalene, the first intermediate in melanin biosynthesis. Cgpks1 albino mutants created by targeted gene disruption were unable to penetrate intact leaves and ruptured frequently but, surprisingly, were able to penetrate ultrathin polytetrafluoroethylene membranes mimicking the plant surface. Nonmelanized Cgpks1 appressoria were sensitive to externally applied cell-wall-degrading enzymes whereas melanized appressoria were not affected. Expression studies using a truncated CgPKS1 fused to green fluorescent protein revealed fluorescence in immature appressoria and in setae, which is in agreement with transcript data obtained by RNA-Seq and quantitative polymerase chain reaction. Unexpectedly, surface scans of mutant and wild-type appressoria revealed considerable differences in cell-wall morphology. Melanization of appressoria is indispensable for successful infection of intact leaves. However, cell collapse experiments and analysis of the appressorial osmolyte content by Mach-Zehnder interferometry convincingly showed that melanin is not required for solute accumulation and turgor generation, thus questioning the role of melanin as a barrier for osmolytes in appressoria of C. graminicola.
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Carruthers, R. I., G. C. Bergstrom, and P. A. Haynes. "Accelerated Development of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Pyralidae), Induced by Interactions with Colletotrichum graminicola (Melanconiales: Melanconiaceae), the Causal Fungus of Maize Anthracnose." Annals of the Entomological Society of America 79, no. 3 (1986): 385–89. http://dx.doi.org/10.1093/aesa/79.3.385.
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Rosas, Juan C., Aracely Castro, and Edwin Flores. "Mejoramiento genético del frijol rojo y negro mesoamericano para Centroamérica y El Caribe." Agronomía Mesoamericana 11, no. 2 (2006): 37. http://dx.doi.org/10.15517/am.v11i2.17305.
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Bean production in Central America is mainly a small farm operation on hillside, marginal areas, limited by several biotic and abiotic constraints. More than 350,000 t of beans are produced in nearly 0.5 millions of hectáreas, with a rather low yield average of 678 kg/ha. The majority of bean producers utilize low inputs and few farms are mechanized. Autoconsumption is rather high; however, a great portion of the beans is sold by intermediaries. In Honduras, beans are the 7th most important crop in economic value, and has the highest economic return among corn, rice and sorghum. The major market classes in Central America are small red and black (race Mesoamerican) beans. Improvement of these bean types is focused in developing resistance to diseases (common mosaic, golden mosaic, anthracnose, angular leaf spot, rust, web blight, and common bacterial blight) and pests (mainly pod weevil); and tolerance to low fertility, drought and heat. Appropriate hybridization and selection programs are utilized for developing multiple resistant, high yielding, well adapted and commercially accepted cultivars. A broad genetic base is accomplished by using Andean and Mesoamerican sources of germplasm in the hybridization stage. Simultaneous selection for various traits and multilocation testing of advanced lines are practiced. Yield and adaptation nurseries and trials of improved lines are distributed to Central America, Mexico, Panama and Caribbean countries. Testing of advanced lines and on-farm validation and varietal release, are carried out by National Bean Programs and institutions, members of the PROFRIJOL regional program, in collaboration with Zamorano, CIAT and the Bean/Cowpea CRSP.
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Islam, F. M. A., K. E. Basford, R. J. Redden, C. Jara, and S. Beebe. "Patterns of resistance to angular leaf spot, anthracnose and common bacterial blight in common bean germplasm." Australian Journal of Experimental Agriculture 42, no. 4 (2002): 481. http://dx.doi.org/10.1071/ea01035.
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Diseases and insect pests are major causes of low yields of common bean (Phaseolus vulgaris L.) in Latin America and Africa. Anthracnose, angular leaf spot and common bacterial blight are widespread foliar diseases of common bean that also infect pods and seeds. One thousand and eighty-two accessions from a common bean core collection from the primary centres of origin were investigated for reaction to these three diseases. Angular leaf spot and common bacterial blight were evaluated in the field at Santander de Quilichao, Colombia, and anthracnose was evaluated in a screenhouse in Popay�n, Colombia. By using the 15-group level from a hierarchical clustering procedure, it was found that 7 groups were formed with mainly Andean common bean accessions (Andean gene pool), 7 groups with mainly Middle American accessions (Middle American gene pool), while 1�group contained mixed accessions. Consistent with the theory of co-evolution, it was generally observed that accessions from the Andean gene pool were resistant to Middle American pathogen isolates causing anthracnoxe, while the Middle American accessions were resistant to pathogen isolates from the Andes. Different combinations of resistance patterns were found, and breeders can use this information to select a specific group of accessions on the basis of their need.
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Araya, Carlos M., and Rodolfo Araya. "Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L.) en Costa Rica." Agronomía Mesoamericana 11, no. 2 (2006): 25. http://dx.doi.org/10.15517/am.v11i2.17302.
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In this research a broad set of bean genotypes were evaluated in the search for sources of resistance to both, anthracnose (Colletotrichum lindemuthianum) and angular leaf spot (Pahaeoisriopsis griseola). The germplasm evaluated came from the National Bean Breeding Program and international nurseries from CIAT: VIFURE, VIPADOGEN and CORE COLLECTION. Experimental plots were establised in Puriscal (1017 masl), Alajuela (814 masl) and Fraijanes (1650 masl), during the 97-98 and 98-99 growing seasons. Experimental plots were single or two rows from two to six m in length depending on seed availability. Disease reaction was scored at six and eight weeks after seeding using a 1 to 9 severity scale. Only genotypes showing 1-3 disease reaction for anthracnose and 1-4 for angular leaf spot were selected. In the germplas from the National Bean Breeding Program nine lines resistant to both pathogens were identified, of those, five lines were specifically resistant to anthracnose and two angular leaf spot. In the VIPADOGEN from CIAT, 25 lines were resistant to anthracnose and only two were resistant to both diseases. These materials also displayed adaptation to either drought or low soil fertility. The CORE COLLECTION nursery provided 82 lines resistant to anthracnose, 12 lines resistant to angular leaf spot and 26 lines were resistant to both pathogens. The most outstanding sources of resistance to anthracnose and angular leaf spot will be made available in a regional nursery.
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Hagan, Austin, and Jackie Mullen. "Occurrence of Dogwood Anthracnose, Spot Anthracnose, and Botrytis Blight in Native Stands of Flowering Dogwood in North Alabama." Journal of Environmental Horticulture 18, no. 3 (2000): 154–59. http://dx.doi.org/10.24266/0738-2898-18.3.154.
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Abstract In North Alabama, dogwood anthracnose (Discula destructiva) was the most common and damaging disease identified in stands of native flowering dogwood (Cornus florida). In the spring of 1992 and 1993, this disease was observed at 59% and 64%, respectively, of the forest and park sites surveyed. Highest incidence and severity of dogwood anthracnose was recorded in the Appalachian Mountains and adjoining foothills of the Piedmont at elevations of 372 to 558 m (1200 to 1800 ft) in the northeastern corner of Alabama near Georgia and Tennessee. At selected sites in Cherokee, Cleburne, DeKalb, Jackson, and Madison Counties, approximately 90% to 100% of the trees examined displayed diagnostic symptoms of dogwood anthracnose. Extensive blighting of the leaves, shoot dieback, epicormic shoot formation, and sometimes tree death were noted. Lower levels of anthracnose damage were recorded on trees in several additional counties in northeast Alabama. Survey results indicate that this disease has not spread onto flowering dogwood in other counties in North Alabama. Spot anthracnose (Elsinoe corni) and Botrytis blight (Botrytis cinerea) were found far less frequently and at fewer locations on flowering dogwood than dogwood anthracnose. Typically, damage attributed to either disease was unobtrusive and of little threat to tree health.
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Sharma, Rajan, H. D. Upadhyaya, S. V. Manjunatha, V. P. Rao, and R. P. Thakur. "Resistance to Foliar Diseases in a Mini-Core Collection of Sorghum Germplasm." Plant Disease 96, no. 11 (2012): 1629–33. http://dx.doi.org/10.1094/pdis-10-11-0875-re.
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Anthracnose, leaf blight, and rust are important biotic constraints to grain and forage sorghum production worldwide and are best managed through host plant resistance. A sorghum mini-core collection, consisting of 242 germplasm accessions developed from a core collection of 2,246 landrace accessions originating from 58 countries, was evaluated to identify sources of resistance to foliar diseases. The mini-core accessions were evaluated in anthracnose- and leaf-blight-screening nurseries under artificial inoculation in the rainy and late rainy seasons, respectively, during 2009 and 2010. For rust resistance, screening was done under artificial inoculation in the greenhouse as well as in the field under natural infection. In all, 13 accessions were found resistant (score ≤3.0 on a 1-to-9 scale) to anthracnose and 27 to leaf blight in both 2009 and 2010. Six accessions exhibited resistance to rust in both the greenhouse and the field. In the resistant accessions, a wide range of diversity was observed for agronomic traits such as days to 50% flowering, plant height, and grain yield/plant, and morphological characteristics such as grain or glume color, glume coverage, endosperm texture, and panicle type (ear head compactness). Three mini-core accessions (IS 473, IS 23684, and IS 23521) exhibited resistance to all three diseases. These accessions with multiple disease resistance will be useful in sorghum disease resistance breeding programs.
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Lewers, K. S., W. W. Turechek, S. C. Hokanson, et al. "Evaluation of Elite Native Strawberry Germplasm for Resistance to Anthracnose Crown Rot Disease Caused by Colletotrichum Species." Journal of the American Society for Horticultural Science 132, no. 6 (2007): 842–49. http://dx.doi.org/10.21273/jashs.132.6.842.
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Anthracnose crown rot of cultivated strawberry (Fragaria ×ananassa Duchesne ex Rozier) has been a major disease problem in the strawberry producing regions of the southeastern United States since the early 1970s. Chemical controls are often inadequate, but use of resistant cultivars is seen as a credible option for managing this disease. Only a small portion of Fragaria L. germplasm has been screened for resistance to anthracnose crown rot. A core subset of the Fragaria collection maintained at the U.S. Department of Agriculture National Clonal Repository in Corvallis, OR, has been constructed to contain an elite group of native F. virginiana Mill. and F. chiloensis (L.) Mill. This collection, referred to as the “core collection,” has been characterized for many horticultural traits, including reactions to several common foliar diseases, resistance to black root rot (causal organisms unknown), and resistance to northern root-knot nematode (Meloidogyne hapla Chitwood) and root-lesion nematode [Pratylenchus penetrans (Cobb) Filipjev & Shuurmans Stekhoven]. Our objective was to evaluate the core collection for resistance to a selection of isolates of three Colletotrichum Corda species known to cause strawberry anthracnose, Colletotrichum fragariae A.N. Brooks, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. [teleomorph Glomerella cingulata (Stoneman) Spauld. & H. Schrenk], and Colletotrichum acutatum J.H. Simmonds (teleomorph Glomerella acutata J.C. Guerber & J.C. Correll). No Fragaria subspecies or geomorph was more resistant than any other; rather, individual genotypes within these groups were identified as sources from which resistance can be obtained. Collecting germplasm in areas of intense disease pressure may not be as beneficial as one might assume, at least where anthracnose crown rot disease is concerned.
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Hagan, A. K., B. Hardin, C. H. Gilliam, G. J. Keever, J. D. Williams, and J. Eakes. "Susceptibility of Cultivars of Several Dogwood Taxa to Powdery Mildew and Spot Anthracnose." Journal of Environmental Horticulture 16, no. 3 (1998): 147–51. http://dx.doi.org/10.24266/0738-2898-16.3.147.
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Abstract Incidence of powdery mildew (Microsphaera penicillata) and spot anthracnose (Elsinoe corni) was assessed on 37 selections or cultivars of flowering dogwood (Cornus florida), kousa dogwood (C. kousa), hybrid dogwood (C. kousa x florida and C. nuttallii x florida) and giant dogwood (C. controversa). Across all cultivars, the flowering dogwood and C. nuttallii x florida ‘Eddie's White Wonder’ are more susceptible to powdery mildew and spot anthracnose than the kousa, C. kousa x florida hybrids and giant dogwood. Among the cultivars of flowering dogwood screened, ‘Cherokee Brave’, ‘Cherokee Chief’, ‘Welch's Bay Beauty’ and ‘Weaver's White’ were partially to highly resistant to both diseases. With few exceptions, the kousa dogwood and C. kousa x florida hybrids, and giant dogwood suffered very little powdery mildew or spot anthracnose-related damage.
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O'Neill, Nichole R., and Gary R. Bauchan. "Sources of Resistance to Anthracnose in the Annual Medicago Core Collection." Plant Disease 84, no. 3 (2000): 261–67. http://dx.doi.org/10.1094/pdis.2000.84.3.261.
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The annual genus Medicago core collection, consisting of 201 accessions, represents the genetic diversity inherent in 3,159 accessions from 36 annual Medicago species. This germ plasm was evaluated for resistance to anthracnose caused by Colletotrichum trifolii. Anthracnose is a major disease in perennial alfalfa (Medicago sativa L.) grown in North America and disease control is based principally on the use of resistant varieties. Evaluation of the core collection was conducted using standardized environmental conditions in growth chambers, and included the M. sativa standard reference cvs. Arc (resistant) and Saranac (susceptible). The degree of resistance found among accessions within species was highly variable; however, most annual species and accessions were susceptible. Only 14 accessions from seven species exhibited resistance greater than 40% seedling survival. These included accessions of M. murex, M. muricoleptis, M. polymorpha var. brevispina, M. polymorpha var. polymorpha, M. radiata, M. soleirolii, M. truncatula, and M. turbinata. Of the 12 accessions of M. polymorpha var. polymorpha, 4 exhibited more than 50% resistance, but 3 accessions were 100% susceptible. Most of the M. truncatula and M. turbinata accessions exhibited significantly more resistance than accessions of other species. Plant introduction (PI) accession number PI 495401 of M. muricoleptis exhibited 90.3% resistance. Accessions of M. scutellata were uniformly susceptible. Histological examinations of 14 of the most anthracnose-resistant accessions revealed that C. trifolii spores germinated and produced typical appressoria, but failed to penetrate and produce the primary and secondary hyphae characteristic of susceptible interactions. Resistant reactions were similar to those found in incompatible interactions with C. trifolii and alfalfa, which have been associated with specific genes leading to the production of isoflavonoid phytoalexins. The large genetic variability in annual Medicago spp. offers potential for locating and utilizing disease resistance genes through breeding or genetic engineering that will enhance the utilization of Medicago spp. as a forage crop.
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Mahuku, George S., Carlos E. Jara, César Cajiao, and S. Beebe. "Sources of Resistance to Colletotrichum lindemuthianum in the Secondary Gene Pool of Phaseolus vulgaris and in Crosses of Primary and Secondary Gene Pools." Plant Disease 86, no. 12 (2002): 1383–87. http://dx.doi.org/10.1094/pdis.2002.86.12.1383.
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Use of genetic resistance is the most practical and economic way to manage anthracnose of common bean. Colletotrichum lindemuthianum, the causal agent of bean anthracnose, is a highly variabile pathogen, and there are no host resistance genes that are effective against all known races of the pathogen. To diversify sources of resistance, we screened the core collection of the secondary gene pool of Phaseolus spp. and interspecific lines derived from simple and complex crosses of primary and secondary genotypes for their resistance to anthracnose. High levels of resistance were observed in the secondary gene pool. None of the 162 accessions tested was susceptible to C. lindemuthianum. Of the two species composing the secondary gene pool, P. polyanthus displayed higher levels of resistance than P. coccineus, and all accessions tested were resistant. The response of P. coccineus was more variable, with six genotypes showing an intermediate reaction. Among the 75 lines from interspecific crosses, 49 were resistant to the three races (races 6, 15, and 3481) used in this study, and higher levels of resistance were found in lines that had P. polyanthus as one of the parents in the crosses than in the lines derived from P. coccineus. These lines constitute a valuable source of resistance and may aid in the development of stable resistance to anthracnose.
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Boersma, J. G., R. L. Conner, P. M. Balasubramanian, K. Yu, and A. Hou. "Marker-assisted dissection of anthracnose resistance in the dry bean cultivar Morden003." Canadian Journal of Plant Science 93, no. 6 (2013): 1115–23. http://dx.doi.org/10.4141/cjps2013-085.
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Boersma, J. G., Conner, R. L., Balasubramanian, P. M., Yu, K. and Hou, A. 2013. Marker-assisted dissection of anthracnose resistance in the dry bean cultivar Morden003. Can. J. Plant Sci. 93: 1115–1123. The dry bean cultivar Morden003 is resistant to anthracnose races 73 and 105, the two most prevalent races in western Canada. Previous studies found that Morden003 carried markers OF10530r, SCAreoli and SAS13 that are linked to the Co-1, Co-2 and Co-4 resistance genes on chromosomes Pv01, Pv11 and Pv08, respectively. Morden003 had a reported resistance spectrum similar to three other cultivars that carry the Co-1 5 resistance gene. Using F2 and F2:3 populations from the reciprocal crosses of Morden003/OAC Rex, we mapped two race-specific resistance gene loci. An examination of known anthracnose resistance and other core markers showed no evidence of resistance being associated with the Co-1, Co-2, or Co-4 loci. Instead, the resistance genes were co-located in the vicinity of the Co-3 locus on Pv04. They were 2 cM apart and flanked by markers SAH181100 and BM161. The map generated in this research also showed strong linkage of the anthracnose resistance loci to markers SW12, PVctt001 and SF10, which were associated with the Co-3 and Co-10 loci by previous researchers. A weak, distant linkage of marker SB12 to the Co-3 locus was also detected.
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Monteagudo, Ana B., A. Paula Rodiño, Margarita Lema, et al. "Resistance to Infection by Fungal, Bacterial, and Viral Pathogens in a Common Bean Core Collection from the Iberian Peninsula." HortScience 41, no. 2 (2006): 319–22. http://dx.doi.org/10.21273/hortsci.41.2.319.
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Availability of germplasm with high level of resistance is essential for broadening the genetic base and breeding crop cultivars resistant to abiotic and biotic stresses. The objective of this study was to determine reaction of a common bean core collection from the Iberian Peninsula to anthracnose, rust, common and halo blights, bean common mosaic virus (BCMV, a potyvirus) and bean common mosaic necrosis virus (BCMNV, a potyvirus) pathogens. Of 43 accessions evaluated, 14 large-seeded Andean type, seven small-seeded Middle American type and seven with intermediate characteristics or recombinant type between the two gene pools had resistant reaction to one or more diseases. Resistance to race 17 or 23 of anthracnose pathogen was present in 17 accessions and four accessions were resistant to both races. Resistance to race 38 or 53 of rust pathogen was shown by 22 accessions and five accessions were resistant to both races. All accessions were susceptible to common bacterial blight and 12 accessions had resistance to halo blight. Ten accessions showed resistance to BCMV, none to BCMNV, and two were variable to both viruses. Accessions such as PHA-0573 (pinto), PHA-0589 (marrow), PHA-0654 (favada pinto), and PHA-0706 (favada) showed resistance to two or more diseases. These accessions may be valuable in breeding Andean bean for enhancing simultaneous utilization of both large seed size and disease resistance.
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Cui, Yaya, Dongqin Chen, Yuexu Jiang, Dong Xu, Peter Balint-Kurti, and Gary Stacey. "Variation in Gene Expression between Two Sorghum bicolor Lines Differing in Innate Immunity Response." Plants 10, no. 8 (2021): 1536. http://dx.doi.org/10.3390/plants10081536.
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Microbe associated molecular pattern (MAMPs) triggered immunity (MTI) is a key component of the plant innate immunity response to microbial recognition. However, most of our current knowledge of MTI comes from model plants (i.e., Arabidopsis thaliana) with comparatively less work done using crop plants. In this work, we studied the MAMP triggered oxidative burst (ROS) and the transcriptional response in two Sorghum bicolor genotypes, BTx623 and SC155-14E. SC155-14E is a line that shows high anthracnose resistance and the line BTx623 is susceptible to anthracnose. Our results revealed a clear variation in gene expression and ROS in response to either flagellin (flg22) or chitin elicitation between the two lines. While the transcriptional response to each MAMP and in each line was unique there was a considerable degree of overlap, and we were able to define a core set of genes associated with the sorghum MAMP transcriptional response. The GO term and KEGG pathway enrichment analysis discovered more immunity and pathogen resistance related DEGs in MAMP treated SC155-14E samples than in BTx623 with the same treatment. The results provide a baseline for future studies to investigate innate immunity pathways in sorghum, including efforts to enhance disease resistance.
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Kaiser, W. J., M. Mihov, F. J. Muehlbauer, and R. M. Hannan. "First Report of Anthracnose of Lentil Incited by Colletotrichum truncatum in Bulgaria." Plant Disease 82, no. 1 (1998): 128. http://dx.doi.org/10.1094/pdis.1998.82.1.128c.
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In June 1992 and 1995, anthracnose of lentil (Lens culinaris Medik.) incited by Colletotrichum truncatum (Schwein.) Andrus & W. D. Moore was widespread in field trials at the Institute for Wheat and Sunflower ‘Dobroudja’ near General Toshevo in northeastern Bulgaria. Lesions on the leaves, stems, and pods were usually white to grayish on younger plants, often turning brown as plants matured. Severe infection usually resulted in dieback and/or death of plants. Acervuli containing spores and dark setae were observed within lesions, and conidia from the acervuli produced pure cultures of C. truncatum. Conidia were hyaline, onecelled, falcate to nearly straight with a prominent clear area in the center of highly granular cytoplasm, and measured 17.6 to 19.8 × 4.4 μm. C. truncatum was seed-borne in naturally infected lentil cv. Tadjikskaya 95 at low frequencies (<2%). Koch's postulates were fulfilled by inoculating the foliage of lentil cvs. Brewer and Pardina and reisolating the fungus from stem and petiole lesions. In pathogenicity tests, three isolates of C. truncatum from the foliage and seeds of lentil caused severe symptoms on inoculated lentil cvs. Brewer and Pardina, similar to those observed on diseased lentils in Bulgaria. The fungus also caused moderate symptoms on inoculated faba bean (Vicia faba L.) and pea (Pisum sativum L.), and light symptoms on inoculated chickpea (Cicer arietinum L.). In 1995, 258 USDA Plant Introduction (PI) accessions from the USDA lentil core collection were screened in replicated trials in northeastern Bulgaria and disease symptoms were observed in >90% of the lines. Anthracnose severity ranged from light to severe. A few accessions appeared to have acceptable levels of resistance to the disease. These included accessions from Iran (PI 431714 and 431717) and Spain (PI 533693). Also that year, C. truncatum was isolated from stem lesions of naturally infected bitter vetch (Vicia ervilia (L.) Willd.) at the Institute for Wheat and Sunflower ‘Dobroudja’. The disease in Bulgaria appears to be identical to one causing anthracnose of lentil in Canada (1) and the United States (2). This is the first report of C. truncatum causing anthracnose of lentil in Bulgaria. References: (1) R. A. A. Morrall. Plant Dis. 72:994, 1988. (2) J. R. Venette et al. Plant Dis. 78:1216, 1994.
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Staub, Jack E., Fenny Dane, Kathleen Reitsma, Gennaro Fazio, and Anabel López-Sesé. "The Formation of Test Arrays and a Core Collection in Cucumber Using Phenotypic and Molecular Marker Data." Journal of the American Society for Horticultural Science 127, no. 4 (2002): 558–67. http://dx.doi.org/10.21273/jashs.127.4.558.
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Genetic relationships among 970 cucumber (Cucumis sativus L.) plant introductions (PIs) in the U.S. National Plant Germplasm System (NPGS) were assessed by observing variation at 15 isozyme loci. Allozyme frequency data for these PIs were compared to allozyme variation in heirloom and modern (H&M) cultivars released from 1846-1985 (H&M cultivars; 178 accessions), and experimental commercial (EC) germplasm (EC germplasm; 82 accessions) in use after 1985. Multivariate analysis defined four distinct groups of accessions (Groups A-D), where Group A consisted of PIs received by the NPGS before 1992, Group B contained PIs from India and China obtained by NPGS after 1992, Group C consisted of EC germplasm, and Group D contained H&M cultivars. Morphological, abiotic stress (water and heat stress tolerance) and disease resistance evaluation data from the Germplasm Resources Information Network (GRIN) for the PIs examined were used in conjunction with estimates of population variation and genetic distance estimates to construct test arrays and a core collection for cucumber. Disease resistance data included the evaluation of angular leafspot [Pseudomonas lachrymans (E.F. Smith) Holland], anthracnose [Colletotrichum lagenarium (Ross.) Ellis & Halst], downy mildew [Pseudoperonospora cubensis (Berk. & Curt) Rostow], rhizoctonia fruit rot (Rhizoctonia solani Kuhn), and target leafspot [Corynespora cassiicola (Berk. & Curt) Wei] pathogenicity. The test arrays for resistance-tolerance to angular leafspot, anthracnose, downy mildew, rhizoctonia fruit rot, target leafspot, and water and heat stress consisted of 17, 16, 17, 16, 17, 16, and 16 accessions, respectively. The core collection consisted of accessions in these test arrays (115) and additional 32 accessions that helped circumscribe the genetic diversity of the NPGS collection. The core collection of 147 accessions (115 + 32) represents ≈11% of the total collection's size (1352). Given estimates of genetic diversity and theoretical retention of diversity after sampling, this core collection could increase curatorial effectiveness and the efficiency of end-users as they attempt to identify potentially useful germplasm.
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Grabowski, Marek Franciszek. "Incidence of postharvest fungal diseases of apples in integrated fruit production." Acta Scientiarum Polonorum Hortorum Cultus 20, no. 1 (2021): 123–29. http://dx.doi.org/10.24326/asphc.2021.1.12.
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In 2014–2017 an investigation was carried out into the occurrence of fungal storage diseases of five apple varieties (Red Jonaprince, Gala, Golden Delicious, Gloster and Ligol) in the Sandomierz orchard region. The fruit was stored at a CA cold storage room with ULO controlled atmosphere for six months. Occurrence of eight storage diseases was found. The most frequently occurring disease was bull’s eye rot and the losses caused thereby were even 24% of the affected fruit. The cultivars most susceptible to this disease were the Golden Delicious and Ligol apples; the least susceptible were the Gloster ones. The apples were significantly less affected by the fungi that cause brown rot, grey mould rot, blue mould rot and apple scab. Very seldom were the symptoms of calyx end rot, mouldy core and core rot, and anthracnose. Varying severity of infection of the varieties was noted in each season of observation.
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Ahn, Ezekiel, Zhenbin Hu, Ramasamy Perumal, et al. "Genome wide association analysis of sorghum mini core lines regarding anthracnose, downy mildew, and head smut." PLOS ONE 14, no. 5 (2019): e0216671. http://dx.doi.org/10.1371/journal.pone.0216671.
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Freeman, Stanley, Ezra Shabi, and Talma Katan. "Characterization of Colletotrichum acutatum Causing Anthracnose of Anemone (Anemone coronaria L.)." Applied and Environmental Microbiology 66, no. 12 (2000): 5267–72. http://dx.doi.org/10.1128/aem.66.12.5267-5272.2000.
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ABSTRACT Anthracnose, or leaf-curl disease of anemone, caused byColletotrichum sp., has been reported to occur in Australia, western Europe, and Japan. Symptoms include tissue necrosis, corm rot, leaf crinkles, and characteristic spiral twisting of floral peduncles. Three epidemics of the disease have been recorded in Israel: in 1978, in 1990 to 1993, and in 1996 to 1998. We characterized 92Colletotrichum isolates associated with anthracnose of anemone (Anemone coronaria L.) for vegetative compatibility (72 isolates) and for molecular genotype (92 isolates) and virulence (4 isolates). Eighty-six of the isolates represented the three epidemics in Israel, one isolate was from Australia, and five isolates originated from western Europe. We divided these isolates into three vegetative-compatibility groups (VCGs). One VCG (ANE-A) included all 10 isolates from the first and second epidemics, and 13 of 62 examined isolates from the third epidemic in Israel, along with the isolate from Australia and 4 of 5 isolates from Europe. Another VCG (ANE-F) included most of the examined isolates (49 of the 62) from the third epidemic, as well as Colletotrichum acutatum from strawberry, in Israel. Based on PCR amplification with species-specific primers, all of the anemone isolates were identified as C. acutatum. Anemone and strawberry isolates of the two VCGs were genotypically similar and indistinguishable when compared by arbitrarily primed PCR of genomic DNA. Only isolate NL-12 from The Netherlands, confirmed as C. acutatum but not compatible with either VCG, had a distinct genotype; this isolate represents a third VCG of C. acutatum. Isolates from anemone and strawberry could infect both plant species in artificial inoculations. VCG ANE-F was recovered from natural infections of both anemone and strawberry, but VCG ANE-A was recovered only from anemone. This study of C. acutatum from anemone illustrates the potential of VCG analysis to reveal distinct subspecific groups within a pathogen population which appears to be genotypically homogeneous by molecular assays.
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Rungjindamai, Nattawut. "Isolation and evaluation of biocontrol agents in controlling anthracnose disease of mango in Thailand." Journal of Plant Protection Research 56, no. 3 (2016): 306–11. http://dx.doi.org/10.1515/jppr-2016-0034.
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Abstract The agricultural based economy is a core business in Thailand and food export is one of the main sources of income for the Thai population. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Mango (Mangifera indica L.) is widely planted in Thailand and is one of the major cash crops for international export. However, mango suffers from various diseases especially anthracnose, a fungal disease caused by Colletotrichum gloeosporioides. One hundred and twelve isolates of epiphytic microbes were isolated from healthy leaves and fruits of mangoes; this included 93 and 19 isolates of epiphytic bacteria and yeasts, respectively. They were screened for bioactivity against a pathogenic strain of C. gloeosporioides isolated from diseased mangoes using a dual culture technique. Out of 112 isolates, eight isolates exhibited at least 60% inhibition. These isolates were further screened for their inhibition on mango using fruit inoculation. Two isolates reduced the lesion sizes caused by C. gloeosporioides compared to control treatment. These two isolates, based on phenotypical and biochemical tests, were identified as Bacillus sp. MB61 and Bacillus sp. LB72.
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Wang, Xiaolian, Dongxiao Lu, and Chengming Tian. "CgEnd3 Regulates Endocytosis, Appressorium Formation, and Virulence in the Poplar Anthracnose Fungus Colletotrichum gloeosporioides." International Journal of Molecular Sciences 22, no. 8 (2021): 4029. http://dx.doi.org/10.3390/ijms22084029.
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The hemibiotrophic ascomycete fungus Colletotrichum gloeosporioides is the causal agent of anthracnose on numerous plants, and it causes considerable economic losses worldwide. Endocytosis is an essential cellular process in eukaryotic cells, but its roles in C. gloeosporioides remain unknown. In our study, we identified an endocytosis-related protein, CgEnd3, and knocked it out via polyethylene glycol (PEG)-mediated protoplast transformation. The lack of CgEnd3 resulted in severe defects in endocytosis. C. gloeosporioides infects its host through a specialized structure called appressorium, and ΔCgEnd3 showed deficient appressorium formation, melanization, turgor pressure accumulation, penetration ability of appressorium, cellophane membrane penetration, and pathogenicity. CgEnd3 also affected oxidant adaptation and the expression of core effectors during the early stage of infection. CgEnd3 contains one EF hand domain and four calcium ion-binding sites, and it is involved in calcium signaling. A lack of CgEnd3 changed the responses to cell-wall integrity agents and fungicide fludioxonil. However, CgEnd3 regulated appressorium formation and endocytosis in a calcium signaling-independent manner. Taken together, these results demonstrate that CgEnd3 plays pleiotropic roles in endocytosis, calcium signaling, cell-wall integrity, appressorium formation, penetration, and pathogenicity in C. gloeosporioides, and it suggests that CgEnd3 or endocytosis-related genes function as promising antifungal targets.
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Liu, B., M. Munster, C. Johnson, and F. J. Louws. "First Report of Anthracnose Caused by Colletotrichum fragariae on Cyclamen in North Carolina." Plant Disease 95, no. 11 (2011): 1480. http://dx.doi.org/10.1094/pdis-06-11-0475.
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In November 2009, cyclamen (Cyclamen persicum) plants with disease symptoms from a commercial greenhouse operation in the western part of North Carolina were sent to the Plant Diseases and Insect Clinic at North Carolina State University. Symptoms consisted of coalescing reddish and tan necrotic leaf spots with concentric circles. Other symptoms included darkened vascular tissue and decay of the corm, large roots, and petioles. Diseased leaves and stems were surface sterilized in 0.5% sodium hypochlorite for 3 min, air dried, and placed in petri dishes containing alkaline water agar. After 3 days of incubation at room temperature, fungal colonies were transferred to acidified potato dextrose agar. Isolation frequency after 5 days was 33% (three of nine pieces) and 16% (one of six pieces) from small leaf spots and petioles, respectively. Pure cultures of isolates were gray and black with abundant, aerial, gray whitish mycelia. Diseased plants were also incubated in a moist chamber at room temperature and sporulation was observed within 7 days. Conidia were tapered with rounded ends and produced in the acervulus and on the tips of setae, which is consistent with the morphology of described isolates of Colletotrichum fragariae. Similar setae were also observed directly on the fine roots of the original sample. The pathogenicity of single-spore cultures was tested by spraying four 2-month-old cyclamen plants with a conidial suspension (106 conidia/ml) and the plants were kept in a humid chamber for 24 h. Noninoculated controls (four plants) were sprayed with distilled water and subjected to the same conditions. The pathogenicity test was also repeated. Inoculated plants and controls were placed in a greenhouse with a temperature range from 22 to 25°C. After 7 to 10 days, symptomatic leaves and stems were observed on all the inoculated plants but not on the control plants. Fungi reisolated from 10 symptomatic leaf tissues had identical morphological features as the original isolates. Fungal DNA was extracted with DNeasy Plant Mini DNA Extraction Kits following the manufacturer's protocol (Qiagen Inc., Valencia, CA). Sequence analysis of the rRNA internal transcribed spacer (ITS) region of the cyclamen isolate (GenBank Accession No. HQ188923), based on the fragment amplified with ITS1 and ITS4 primers, showed 100% similarity to isolates of C. fragariae deposited in GenBank (Accession Nos. FJ172290 [ATCC MYA-4443 from cyclamen] and FJ810510 [ATCC MYA-4442 from silver date palm]) and Florida isolate C16 isolated from strawberry (1). In addition, the morphology and ITS sequences of the cyclamen isolate were identical to those of the C. fragariae voucher isolate from strawberry (GU174546). Results from disease symptoms, colony and spore morphology, pathogenicity tests, and ITS sequence analysis suggest that C. fragariae was the pathogen responsible for the disease symptoms on cyclamens. To our knowledge, this is the first report of a disease caused by C. fragariae on cyclamen in North Carolina and complements an earlier report from Florida (1). Reference: (1) S. J. MacKenzie et al. Plant Dis. 92:1432, 2008.
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Johnson, Monte P., John R. Hartman, Robert E. McNiel, and William M. Fountain. "Evaluation of Dogwood and Birch Species and Cultivars for Resistance to Key Insect Pests and Diseases." Journal of Environmental Horticulture 19, no. 2 (2001): 73–78. http://dx.doi.org/10.24266/0738-2898-19.2.73.
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Abstract Ten cultivars of dogwoods (Cornus spp.) were evaluated in multi-year trials for relative reisistance to the dogwood borer, Synanthedon scitula (Harris); cold injury; canker disease, Botryosphaeria dothidea; spot anthracnose, Elsinoe corni; and powdery mildew, Oidium sp., Microsphaera sp., and Phyllactinia sp. Similarly, eight cultivars of birch (Betula spp.) were evaluated for resistance to the birch leafminer, Fenusa pusilla (Lepeletier); Japanese beetle, Popillia japonica Newman; aphids, Hamamelistes spinosus Shimer; the bronze birch borer, Agrilus anxius Gory; and leaf-spot, Cryptocline betularum. All cultivars of C. florida, C. kousa and C. kousa x florida were susceptible to dogwood borer, although the C. florida cultivars were surviving better than the others. Cornus mas and C. kousa cultivars were relatively resistant to powdery mildew while C. florida x kousa hybrids and C. florida ‘Cherokee Brave’ were intermediately resistant. Betula platyphylla szechuanica ‘Purpurea’ was highly susceptible to the bronze birch borer, whereas B. nigra and B. n. ‘Heritage’ were the most susceptible birches to aphid damage. Betula jacquemontii was highly susceptible to Japanese beetle defoliation. Betula pendula, B. nigra, and B. n. ‘Heritage’ were most susceptible to defoliation by birch leaf spot. This study suggests that dogwood and birch cultivars vary in susceptibility to key insect pests and diseases. Planting relatively resistant cultivars may be useful in managing perennial pests in urban landscapes.
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He, Chengyong, Ke Duan, Liqing Zhang, et al. "Fast Quenching the Burst of Host Salicylic Acid Is Common in Early Strawberry/Colletotrichum fructicola Interaction." Phytopathology® 109, no. 4 (2019): 531–41. http://dx.doi.org/10.1094/phyto-02-18-0043-r.
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The fungus Colletotrichum fructicola (a species of C. gloeosporioides complex) causes devastating anthracnose in strawberry. Like other species of the genus Colletotrichum, it uses a composite strategy including both the biotrophic and necrotrophic processes for pathogenesis. Host-derived hormones are central regulators of immunity, among which salicylic acid (SA) is the core defense one against biotrophic and hemibiotrophic pathogens. However, the manner and timing of pathogen manipulation of SA are largely elusive in strawberry. To achieve better understanding of the early challenges that SA-mediated defense experiences during strawberry/C. fructicola interaction, dynamic changes of SA levels were followed through the high-performance liquid chromatography method. A very early burst of free SA at 1 h postinoculation (hpi) followed by a fast quenching during the next 12 h was noticed, although rhythm variations were present in two hosts. Transcriptional characterization of genes related to SA pathway in two varieties on C. fructicola inoculation revealed that these genes were differentially expressed, although they were all induced at different time points. At the same time, three types of genes encoding homologous effectors interfering with SA accumulation were found to be first inhibited but sequentially activated during the first 24 hpi. Furthermore, subcellular localization analysis suggests that CfShy1 is a weapon of C. fructicola for strawberry invasion. Based on these results, we propose that the infection strategy that C. fructicola utilizes on strawberry is specialized, which is implemented through the optimized expression of a specific set of effector genes. Transcriptional characterization of host genes supports that de novo SA biosynthesis and the free SA release from methyl salicylate might contribute equally to the intricate control of SA homeostasis in strawberry. C. fructicola manipulation of SA-dependent resistance in strawberry might be closely related to multihormonal interplay among SA, jasmonic acid, abscisic acid, and cytokinin.
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Krut, M. "Innovations on scientific support of grain crop breeding for resistance to diseases and pests." Interdepartmental Thematic Scientific Collection of Plant Protection and Quarantine, no. 66 (December 24, 2020): 137–45. http://dx.doi.org/10.36495/1606-9773.2020.66.137-145.
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Goal. Database formation of innovations in the scientific provision of breeding crops for resistance to pathogens and pests — grounds for creating sustainable varieties.
 Methods. Analysis of innovative development of the Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine and other institutions of the Scientific and Methodological Center «Protection of Plants» for 2001—2020. Isolation of those relating to the problem of grain crops’ resistance to pests and pathogens.
 Results. Methods of selection of wheat and barley for resistance to basic pathogens of diseases and methodology for assessing the stability of winter wheat stability to pests to create complex resistant varieties are developed. A database for the resistance of corn hybrids against main pests was created. Methods of determining the stability of grain crops to high and low temperatures are developed. A collection of samples of wild relative of wheat Aegilops biuncialis L. that are sources of new genes for plant resistance to diseases and pests was created. Genes of resistance of soft winter wheat to diseases by DNA markers have been identified. A set of varieties of winter wheat with group and complex resistance to diseases and pests has been formed. Physiological and biochemical mechanisms of soybean resistance to pathogens of diseases are established. Collection of soybean lines is created on the basis of complex resistance to white rot and anthracnosis. The varieties and selection numbers of spring barley, oats, resistant to basic diseases, as well as rice varieties that are resistant to pathogens of diseases and pests were identified. Resistance to viral diseases has been found in barley plants.
 Conclusions. The established innovations can be widely used by breeding centers and other scientific institutions of the agrarian profile when of output sustainable grain and leguminous grain crops. In this case, the timing of the selection process can be accelerated by 40—60%.
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Oszust, Karolina, Michał Pylak, and Magdalena Frąc. "Trichoderma-Based Biopreparation with Prebiotics Supplementation for the Naturalization of Raspberry Plant Rhizosphere." International Journal of Molecular Sciences 22, no. 12 (2021): 6356. http://dx.doi.org/10.3390/ijms22126356.
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The number of raspberry plants dying from a sudden outbreak of gray mold, verticillium wilt, anthracnosis, and phytophthora infection has increased in recent times, leading to crop failure. The plants suffer tissue collapse and black roots, symptoms similar to a Botrytis–Verticillium–Colletotrichum–Phytophthora disease complex. A sizeable number of fungal isolates were acquired from the root and rhizosphere samples of wild raspberries from different locations. Subsequent in vitro tests revealed that a core consortium of 11 isolates of selected Trichoderma spp. was the most essential element for reducing in phytopathogen expansion. For this purpose, isolates were characterized by the efficiency of their antagonistic properties against Botrytis, Verticillium, Colletotrichum and Phytophthora isolates and with hydrolytic properties accelerating the decomposition of organic matter in the soil and thus making nutrients available to plants. Prebiotic additive supplementation with a mixture of adonitol, arabitol, erythritol, mannitol, sorbitol, and adenosine was proven in a laboratory experiment to be efficient in stimulating the growth of Trichoderma isolates. Through an in vivo pathosystem experiment, different raspberry naturalization-protection strategies (root inoculations and watering with native Trichoderma isolates, applied separately or simultaneously) were tested under controlled phytotron conditions. The experimental application of phytopathogens attenuated raspberry plant and soil properties, while Trichoderma consortium incorporation exhibited a certain trend of improving these features in terms of a short-term response, depending on the pathosystem and naturalization strategy. What is more, a laboratory-scale development of a biopreparation for the naturalization of the raspberry rhizosphere based on the Trichoderma consortium was proposed in the context of two application scenarios. The first was a ready-to-use formulation to be introduced while planting (pellets, gel). The second was a variant to be applied with naturalizing watering (soluble powder).
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Rooney-Latham, S., L. L. Gallegos, P. M. Vossen, and W. D. Gubler. "First Report of Neofabraea alba Causing Fruit Spot on Olive in North America." Plant Disease 97, no. 10 (2013): 1384. http://dx.doi.org/10.1094/pdis-04-13-0394-pdn.
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Olive (Olea europaea) is a widely planted evergreen tree primarily grown for its oil, fruit for pickling, and landscape appeal in Mediterranean and temperate climates. California produces most of the olives grown in the United States; its industry was valued at $53 million in 2011 (4). In 2005 and 2008, fruit spotting occurred on coratina and picholine cultivars in two commercial orchards in Sonoma County. The spots were scattered, slightly sunken and brown, and surrounded by a green halo. Many of the spots were associated with lenticels. A slow to moderate growing, cream to rose-colored fungus was isolated from the spots onto potato dextrose agar (PDA) amended with 0.01% tetracycline hydrochloride. Sporulation was observed in vitro on PDA after 40 days under near-UV light. Macroconidia, produced from conidiomata, were hyaline, aseptate, cylindrical to fusiform-allantoid, slightly curved, and 17 to 27 × 2.5 to 3.5 μm (average 21.1 × 2.9 μm). Microconidia were aseptate, strongly curved, hyaline, and 14 to 18 × 0.75 to 1 μm (average 16.1 × 0.9 μm). rDNA sequences of the internal transcribed spacer (ITS) region of the isolate (GenBank KC751540), amplified using primers ITS1 and ITS4, were 99.8% identical to Neofabraea alba (E.J. Guthrie) Verkley (anamorph Phlyctema vagabunda) (=Gloeosporium olivae) (AF141190). Pathogenicity was tested on detached, green fruit (cv. frantoio). Olives were surface sterilized in 10% sodium hypochlorite for 5 min and air dried. Five olives were wounded with a needle and 10 μl spore suspension (105 spores/ml) was placed on each wound. An equal amount of spore suspension was placed on five unwounded olives. Water was also placed on wounded and unwounded olives to serve as a control. The olives were placed on racks in 22.5 × 30 cm crispers lined with wet paper towels and incubated at 23°C. After 21 days, the olives began to turn red. Olives wounded and inoculated with N. alba had a distinct green ring around the inoculation point where maturity was inhibited. Control olives uniformly turned red. After 35 days, wound-inoculated olives began to form a sunken, brown lesion at the inoculation point where aerial mycelium was visible. After 51 days, lesions were visibly sunken and immature conidiomata began to form in concentric rings giving a bull's eye-like appearance. Unwounded fruit exhibited uneven maturity and green spots associated with the lenticels throughout the experiment but did not develop sunken lesions. Control fruit showed no symptoms and ripened normally. After 56 days, fruit was surface sterilized in 10% sodium hypochlorite for 5 min and plated onto PDA. N. alba was isolated from the sunken and green areas of all of the wounded and unwounded fruit. No fungi grew from the control fruit. The experiment was repeated once with similar results. N. alba has been reported to cause an anthracnose disease on fruit and leaves of olives in Spain and Italy (1,2). In North America, N. alba causes a bull's eye rot on fruit of Malus and Pyrus spp. in the Pacific Northwest and coin canker of Fraxinus spp. in Michigan and Canada (3). To our knowledge, this is the first report of N. alba causing disease on olive in North America. References: (1) J. Del Maral de la Vega et al. Bol. San Veg. Plagas. 12:9. 1986. (2) S. Foschi. Annali. Sper. Agr., n.s. 9:911. 1955. (3) T. D. Gariepy et al. Can. J. Plant Pathol. 27:118. 2005. (4) United States Department of Agriculture, National Agricultural Statistics Service, California Field Office, California Agriculture Statistics, Crop Year 2011.
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Pandey, Abhay K., Ashwani K. Basandrai, Daisy Basandrai, et al. "Field-Relevant New Sources of Resistance to Anthracnose Caused by Colletotrichum truncatum in a Mungbean Mini-Core Collection." Plant Disease, August 5, 2021, PDIS—12–20–2722. http://dx.doi.org/10.1094/pdis-12-20-2722-re.
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Anthracnose is a prevalent disease of mungbean in Asian countries and Sub-Saharan Africa. It is caused by multiple Colletotrichum species. The high levels of anthracnose resistance in mungbean have not been studied in depth in India, but genetic resistance is desired. In this study, we identified the causal agent of mungbean anthracnose in two regions of India as Colletotrichum truncatum through morphological and molecular methods. A set of 296 mungbean mini-core accessions developed by WorldVeg was screened under a natural disease pressure from July to September (kharif season) in 2016, 2017, and 2018 in Hyderabad (a hot spot for anthracnose) to identify anthracnose resistance. Based on disease severity scores, 22 accessions were consistently anthracnose resistant under the categories of immune, highly resistant, and resistant with scores ranging from ≥1.0 to ≤3.0 during the period of study. Furthermore, based on the agronomic performance, anthracnose resistance in Hyderabad, and other desirable traits, a subset of 74 mungbean accessions was selected from 296 mini-core accessions. These accessions were evaluated under natural disease pressure from July to September in 2018 and 2019 in Palampur (another hot spot for anthracnose) to determine the variation in anthracnose resistance. Out of the 74 accessions, two accessions were resistant in 2018; in 2019, one was immune, nine were highly resistant, and 15 were resistant. Combined analysis of variance of 65 accessions common in Hyderabad and Palampur revealed highly significant effects of environment, genotype (accessions), and genotype × environment interaction on the disease severity. The combined GGE biplot analysis of data across years and locations confirmed that the seven accessions MC-24, MC-51, MC-75, MC-127, MC-207, MC-208, and MC-292 were resistant during 2016 to 2018 in Hyderabad, and only in 2019 in Palampur, and the same accessions were moderately resistant in 2018 in Palampur. The seven resistant accessions identified from both test locations could be used as potential donors in the anthracnose resistance breeding program. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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Carbone, María Julia, Victoria Moreira, Pedro Mondino, and Sandra Alaniz. "First report of Anthracnose on Peach Fruit Caused by Colletotrichum siamense in Uruguay." Plant Disease, June 18, 2021. http://dx.doi.org/10.1094/pdis-05-21-0950-pdn.
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Peach (Prunus persica L.) is an economically important deciduous fruit crop in Uruguay. Anthracnose caused by species of the genus Colletotrichum is one of the major diseases in peach production, originating significant yield losses in United States (Hu et al. 2015), China (Du et al. 2017), Korea (Lee et al. 2018) and Brazil (Moreira et al. 2020). In February 2017, mature peach fruits cv. Pavia Canario with symptoms resembling anthracnose disease were collected from a commercial orchard located in Rincon del Colorado, Canelones, in the Southern region of Uruguay. Symptoms on peach fruit surface were characterized as circular, sunken, brown to dark-brown lesions ranging from 1 to 5 cm in diameter. Lesions were firm to touch with wrinkled concentric rings. All lesions progressed to the fruit core in a V-shaped pattern. The centers of the lesions were covered by orange conidial masses. Monosporic isolates obtained from the advancing margin of anthracnose lesions were grown on PDA at 25ºC and 12h photoperiod under fluorescent light. The representative isolates DzC1, DzC2 and DzC6 were morphologically and molecularly characterized. Upper surface of colonies varied from white or pale-gray to gray and on the reverse dark-gray with white to pale-gray margins. Conidia were cylindrical, with both ends predominantly rounded or one slightly acute, hyaline and aseptate. The length and width of conidia ranged from 9.5 to 18.9 µm (x ̅=14.1) and from 3.8 to 5.8 µm (x ̅=4.6), respectively. The ACT, βTUB2, GAPDH, APN2, APN2/MAT-IGS, and GAP2-IGS gene regions were amplified and sequenced with primers ACT-512F/ACT-783R (Carbone and Kohn, 1999), BT2Fd/BT4R (Woudenberg et al. 2009), GDF1/GDR1 (Guerber et al. 2003), CgDLR1/ColDLF3, CgDLF6/CgMAT1F2 (Rojas et al. 2010) and GAP1041/GAP-IGS2044 (Vieira et al. 2017) respectively and deposited in the GenBank database (MZ097888 to MZ097905). Multilocus phylogenetic analysis revealed that Uruguayan isolates clustered in a separate and well supported clade with sequences of the ex-type (isolate ICMP 18578) and other C. siamense strains (isolates Coll6, 1092, LF139 and CMM 4248). To confirm pathogenicity, mature and apparently healthy peach fruit cv. Pavia Canario were inoculated with the three representative isolates of C. siamense (six fruit per isolate). Fruit were surface disinfested with 70% ethanol and wounded with a sterile needle at two equidistant points (1 mm diameter x 1 mm deep). Then, fruit were inoculated with 5 µl of a spore suspension (1×106 conidia mL-1) in four inoculation points per fruit (two wounded and two unwounded). Six fruit mock-inoculated with 5 µl sterile water were used as controls. Inoculated fruit were placed in moist chamber and incubated at 25°C during 10 days. Anthracnose lesions appeared at 2 and 4 days after inoculation in wounded and unwounded points, respectively. After 7 days, disease incidence was 100% and 67% for wounded and unwounded fruit, respectively. The control treatment remained symptomless. The pathogens were re-isolated from all lesions and re-identified as C. siamense. C. siamense was previously reported in South Carolina causing anthracnose on peach (Hu et al. 2015). To our knowledge, this is the first report of anthracnose disease on peach caused by C. siamense in Uruguay. Effective management strategies should be implemented to control anthracnose and prevent the spread of this disease to other commercial peach orchards.
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Cuevas, Hugo E., and Louis K. Prom. "Evaluation of genetic diversity, agronomic traits, and anthracnose resistance in the NPGS Sudan Sorghum Core collection." BMC Genomics 21, no. 1 (2020). http://dx.doi.org/10.1186/s12864-020-6489-0.
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Kamfwa, Kelvin, Paul Gepts, Swivia Hamabwe, Zombe Kapata Nalupya, Chikoti Mukuma, and Davis Lungu. "Characterization of Colletotrichum lindemuthianum Races in Zambia and Evaluation of the CIAT Phaseolus Core Collection for Resistance to Anthracnose." Plant Disease, May 14, 2021. http://dx.doi.org/10.1094/pdis-02-21-0363-re.
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Colletotrichum lindemuthianum, the causal pathogen of common bean (Phaseolus vulgaris) anthracnose, is highly variable. Therefore, understanding its race structure and identification of new sources of resistance is necessary for the development of varieties with durable resistance. The objectives of this study were (i) to characterize isolates of C. lindemuthianum collected from three major bean-growing regions in Zambia, and (ii) evaluate the CIAT Phaseolus core collection for resistance to C. lindemuthianum races 37, 73, and 566, and a blend of 20 races. Isolates collected from three major bean-growing districts in Zambia, namely Mporokoso, Mpika, and Mbala, were characterized as race 37, 73, and 566, respectively. A subset of the CIAT core collection comprised of 885 accessions of common bean, 13 accessions of scarlet runner bean (P. coccineus), and 11 accessions of year bean (P. dumosus) were evaluated for resistance to races 37, 73 and 566, and a blend of 20 races in a greenhouse at University of Zambia, Lusaka, Zambia. A total of 72%, 66%, 48% and 9% of P. vulgaris accessions evaluated were highly resistant to races 37, 73, 566 and a blend of 20 races, respectively. Also, accessions of P. coccineus and P. dumosus, highly resistant to races 37, 73 and 566, were identified. Only eight of the 331 P. vulgaris accessions were highly resistant to all three individual races (37, 73, and 566) and to a blend of 20 races. These eight accessions constitute a valuable breeding resource for developing varieties with durable resistance to C. lindemuthianum.