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1
Thurston, Graham S. (Graham Stanley) 1958. "Host plant resistance and entomogenous nematodes for controlling the northern corn rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae)." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66175.
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Chauhan, Ramola. "A study of filamentous viruses in maize and smallgrains." Master's thesis, University of Cape Town, 1985. http://hdl.handle.net/11427/22013.
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Bibliography: pages 175-184.The occurrence of maize dwarf mosaic virus (MDMV) in field grown maize was investigated. For this purpose, maize showing mosiac symptoms was collected from different maize growing areas in South Africa by Prof. M.B. von Wechmar. These samples from Transvaal, Orange Free State and Natal were then investigated for the presence of MDMV and possible strains of this virus. Three virus isolates were purified and partially characterised. These isolates were serologically compared together with a fourth isolate SCMV 4975, obtained from the U.S., to establish strain relationships.
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Midgarden, David Gunder. "Spatial distribution of western corn rootworm (Diabrotica virgifera virgifera Leconte) trap counts and evaluation of a novel method for sampling soil pests of field corn." Thesis, This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-06302009-040212/.
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Jackson, Tori Lee. "Alternative Controls for Helicoverpazea on Sweet Corn: Phytotoxicity and Pollination Inhibition from Direct Silk Applications." Fogler Library, University of Maine, 2004. http://www.library.umaine.edu/theses/pdf/JacksonTL2004.pdf.
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Fandohan, Pascal. "Fusarium infection and mycotoxin contamination in preharvest and stored maize in Benin, West Africa." Thesis, University of Pretoria, 2004. http://hdl.handle.net/2263/24999.
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Obopile, Motshwari. "INTERACTIONS AMONG MAIZE PHENOLOGIES, TRANSGENIC BACILLUS THURINGIENSIS MAIZE AND SEED TREATMENT FOR MANAGEMENT OF PESTS AND DISEASES OF MAIZE." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243020914.
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Tremblay, Annie C. "The role of birds as predators and potential biocontrol agents of insect pests in corn fields." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ50898.pdf.
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Presello, Daniel A. "Studies on breeding of maize for resistance to ear rots caused by Fusarium spp. and on the occurrence of viruses in maize in eastern Canada." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38260.
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Responses from pedigree selection for resistance to gibberella ear rot were assessed in four maize (Zea mays L.) populations, two selected after inoculation of Fusarium graminearum (Schwabe) macroconidia into the silk channel and two selected after inoculation into developing kernels. Responses were significant in both populations selected for silk resistance and in one of the populations selected for kernel resistance. Selection was more effective in later generations and genetic gains were associated with among-family selection but not with within-family selection. Results obtained here indicate that responses to selection could be more efficiently obtained by applying high selection intensities in advanced generations, by managing earlier generations as bulks and by reducing the number of plants per family. In another experiment, a wide sample of Argentine maize germplasm was evaluated for silk and kernel resistance to gibberella ear rot and to fusarium ear rot (caused by F. verticillioides (Saccardo) Nirenberg [=F. moniliforme (Sheldon)]. Several entries exhibited disease resistance in comparison with local check hybrids, particularly for fusarium ear rot, the most prevalent ear rot in Argentina. Results obtained in this study suggested the presence of general mechanisms controlling silk and kernel resistance to both diseases. In a supplementary study, viral diseases were surveyed in maize fields from the provinces of Ontario and Quebec in 1999 and 2000. Barley yellow dwarf was found in 1999. Sugarcane mosaic, maize dwarf mosaic and wheat streak mosaic were found in 2000. These diseases were not important for grain-maize planted in May, the most prevalent kind of maize crop in these provinces. Some of these diseases, such as sugarcane maize mosaic and maize dwarf mosaic were found important only in maize fields planted during or after the month of June, and this is of commercial relevance only for sweet corn.
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Kadango, Tendayi Lovemore. "Farmers' perceptions of insect pests in the Eastern Cape maize-based cropping systems and the effects of crop residue management on insect pest populations." Thesis, University of Fort Hare, 2018. http://hdl.handle.net/10353/6202.
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Maize (Zea mays L.) is one of the most important crops in South Africa that is cultivated for food, feed and as a cash crop. Maize in the Eastern Cape is typically grown for consumption and the Province is one of the least producers owing to small landholdings, poor production conditions and pests. This study investigated: (i) the different maize cropping systems smallholder farmers practice in the Eastern Cape (EC), (ii) the major maize insect pests within the identified cropping systems, (iii) the perceptions of Eastern Cape smallholder maize farmers on the effects of insect pests, and (iv) the effects of crop residues on insect pests’ population dynamics. Four major cropping systems namely: maize sole cropping without rotations by government sponsored farmers (GCP) (94 percent), maize intercropping without rotations by independent farmers (IF) (79 percent), maize sole cropping without rotations by IF (67 percent) and maize intercropping without rotations by GCP (61 percent) were identified. The choice of a cropping system by the smallholder farmers was significantly influenced by source of sponsorship, the difference in their district localities, tenure system, availability of inputs and farming equipment, percentage of farmland farmers allocate to maize, maize varieties, farming experience, fallow operations and access to irrigation facilities. The major constraints faced by the farmers in maize production are the attack by insect pests, weeds competition, drought, the lack of fencing around the fields and destruction by mammals. The major insect pests identified were stalk borers (Busseola fusca, Chilo partellus), cutworms (Agrotis spp.), aphids (Rhopalosiphum maidis), bollworms (Helicoverpa armigera) and weevils (Sitophylus spp.). The most popular control measures employed were the use of GM maize and pesticides. Results from the field trial showed that field crickets were the major ground dwelling insect pests observed followed by bollworms and cutworms. However, there were no significant influence of residue retention on the insects’ diversity indices, richness, and evenness. Above ground insects sampling revealed the domination by four major insects namely, cutworms, maize stalk borer, bollworms, and spotted stalk borer. Residue management significantly influenced the infestation by spotted stalk borer only on maize plots. However, the general trend was that there was less infestation by the insects in plots where residues were retained than the ones with residue removed. Results from the study indicate that government sponsorship is significantly influencing the maize cropping systems of the smallholder farmers of the Eastern Cape.
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Traut, Eduardo Jorge. "Bipolaris zeicola: physiological races, morphology and resistance on maize." Diss., Virginia Tech, 1993. http://hdl.handle.net/10919/40449.
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Gelderblom, Wentzel Christoffel Andreas. "Mycotoxicological properties of fusarium verticillioides and the fumonisins : mechanisms and implications for setting risk assessment parameters in humans." Thesis, Stellenbosch : Stellenbosch University, 2009. http://hdl.handle.net/10019.1/3971.
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Thesis (DSc (Biochemistry))--Stellenbosch University, 2009.The fumonisin mycotoxins are known to be the causative principle for several animal diseases and are associated with the development of liver and oesophagus cancer and neural tube defects in humans. The thesis focuses mainly on the characterisation of the compounds from maize cultures of the fungus Fusarium verticillioides, isolated from maize, the toxicological effects in animals, mechanism involved in hepato- and nephrocarcinogenicity and discussing the major differences and contradictions in the literature together with their impact on setting relevant risk assessment parameters to safeguard human health. Controversies include the importance of non-genotoxicity vs genotoxicity in the development of cancer, the role of threshold effects in carcinogenesis and the establishment of realistic risk assessment parameters that will also be applicable in developing countries. Recent approaches suggest that thresholds should also apply for genotoxic carcinogens as interaction with the DNA is only one event in the multi-step process of cancer development and therefore could not be taken as the basis for applying a no-effect threshold for genotoxins. It would appear that a carcinogen such as fumonisin, whether it is labeled genotoxic or non-genotoxic per se, exhibits some degree of risk at any level due to additive or synergistic interactions with other xenobiotics and/or dietary constituents. The underlying mechanisms of fumonisin-induced carcinogenicity includes the disruption of sphingolipid, phospholipids and fatty acid metabolism, which plays a major role in the modulation of apoptotic and cell proliferative pathways related to cancer development. Interactive responses between arachidonic acid and ceramide affect downstream cell signal transduction pathways and depending on the cell type the disruption of these pathways could either stimulate or inhibit cell proliferation which eventually will determine the induction of apoptosis and hence affect cell survival. The modulating roles of dietary constituents such as vitamins, protein and the South African herbal teas are also highlighted as they affected the outcome of toxicological assays, thus determining thresholds of the adverse effects in specific target organs that will impact risk assessment parameters. Regulation of the fumonisins in food and the associated risk are debated from many perspectives. In developing countries there is a lack of quality control implying that maize highly contaminated with mycotoxins may directly enter the food chain of adults and children as control of mycotoxins is difficult or in some cases totally absent. The interaction of politics, economy and technology will eventually determine the impact on health as the regulation of fumonisin in food differs between countries. Knowledge about the biological effects of the fumonisins is currently playing an important role in the development of simple and inexpensive methods to reduce the levels of the fumonisin in maize by targeting specific populations at risk.
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Highland, H. Brett. "Ecology of the stalk borer Papaipema nebris (Guenee), (Lepidoptera:noctuidae), in the southwestern Virginia no-till corn agroecosystem." Diss., Virginia Polytechnic Institute and State University, 1986. http://hdl.handle.net/10919/49794.
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Dupeux, Yann Alain. "The effect of cultivation and intercropping on the incidence of ear rot of corn and head blight of wheat." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23269.
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Three cultivators, Rabewerk, Kongskilde and Hiniker, and three intercrops, soybean, lupin and red clover + rye grass were investigated for their impact on the incidence of ear rot of corn, a common disease of maize in eastern Canada. Wheat was seeded in the corn rows to serve as an additional indicator of cultivation and intercropping effects on the pathogen. An artificial inoculum of F. graminearum that produced perithecia and ascospores was used to mimic natural inoculum.In 1993 and 1994, the infection in the corn was not very severe and there were no differences between the treatments and the controls.
In 1993 and 1994, at both sites, wheat seeds from cultivation trials showed a tendency for greater disease incidence in the non-cultivated herbicide treatment when compared to any of the other cultivator treatments. Cultivators till the soil and bury corn residues, this action led to the destruction of some of the inoculum and a subsequent reduction of the disease incidence in the cultivated plots.
In the intercrop trial of 1993 and 1994, wheat infection was moderate to severe, except at L'Assomption in 1993, but no significant differences were observed among the treatments. It is believed that interplot interference, due to ascospores moving from one plot to the next, masked differences.
The results indicated that weed cultivation would have a negligible or no effect on the development of fusarium ear rot of corn in Quebec. (Abstract shortened by UMI.)
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Brunet, Francine. "Intercropping and mechanical weeding : effects on insects of Zea mays." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23990.
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A study was undertaken from 1993 to 1994 to determine the effects of intercropping (with Glycine max L Merr., Lupinus sp., Trifolium pratense L.-Lolium multiflorum Lam., or Secale cereale L.) and mechanical weeding (with a rigid tines cultivator, Danish tines cultivator or ridge cultivator) on the population dynamics of insect pests (Diabrotica longicornis Say, Lygus lineolaris Palisot de Beauvois, Ostrinia nubilalis Hubner, and Pseudaletia unipuncta Haworth) on corn (Zea mays L.) in L'Assomption and Sainte-Anne-de-Bellevue, Quebec, Canada.D. longicornis or P. unipuncta populations did not increase.
In 1994, intercropping with T. pratense-L. multiflorum (Sainte-Anne-de-Bellevue) or weeding with the Danish tines cultivator (L'Assomption) increased L. lineolaris populations.
In 1994, intercropping with S. cereale (L'Assomption) ot T. pratense-L. multiflorum (Sainte-Anne-de-Bellevue) increased O. nubilalis populations.
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Donahue, Patrick J. "Inheritance of reactions to gray leaf spot and maize dwarf mosaic virus in maize and their associations with physiological traits." Diss., Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/54518.
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Gray leaf spot, caused by Cercospora zeae-maydis, can be a yield-limiting factor in maize where continuous minimum tillage practices are followed. Commercial corn hybrids were evaluated for response to gray leaf spot for seven years at two Virginia locations (Shenandoah and Wythe Counties) and one year at a third location in Virginia (Montgomery County). Yield losses, when comparing resistant to susceptible classes, were approximately 2,000 kg ha⁻¹ at Wythe County in 1982, 750 kg ha⁻¹ at Shenandoah County in 1984, and 2,150 kg ha⁻¹ at Montgomery County in 1988. The inheritance of reaction to gray leaf spot was studied using a 14 inbred diallel in Montgomery and Wythe Counties, Virginia in 1987 and 1988 planted in randomized complete block designs. Resistance was found to be highly heritable and controlled by additive gene action. Inbreds producing high yielding, resistant, and agronomically superior hybrids were identified (B68, NC250, Pa875, Va14, Va17, and Va85); and several hybrids between these lines had high levels of resistance, high yield, and good general agronomic characters (B68 x KB1250, KB1250 x Pa875, and NC250 x Pa875). Currently available inbreds could be used to produce hybrids with higher levels of resistance than hybrids currently available to growers, and these could serve as a basis for gray leaf spot breeding programs. Lesion size measurements were not correlated with disease scores. Late-season photosynthesis rates were associated positively with resistance. The hybrids of some inbreds were found to produce high levels of pigment (believed to be anthocyanins) around the gray leaf spot lesions. These did not limit the size of the individual lesion later in the season. Some pigment(s)-producing genotypes were found to be resistant when the pigment character was expressed. This type of resistance must prevent or inhibit infection of the leaf but not later colonization, once established. Maize dwarf mosaic virus (MDMV) also limits maize production in some areas where johnsongrass (Sorghum halepense L.) is a problem. Resistance to MDMV was found to be mainly additive and highly heritable. However, a strong specific combining ability component was found, indicating that the background of the material receiving resistance genes may have a strong effect on the expression of resistance. Inbreds capable of producing high-yielding, resistant, and agronomically acceptable hybrids are available (B68, NC250, A632, Pa875, Va17, and Va85); and several hybrids between these lines have high levels of resistance, high yield, and good general agronomic characters (B68 x KB1250, KB1250 x Pa875, and NC250 x Pa875).Ph. D.
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Skenjana, Nolitha Leonora. "Identification and documentation of ethnobiological methods used by rural farmers to control stalk borers on maize in the Eastern Cape Province of South Africa." Thesis, University of Fort Hare, 2015. http://hdl.handle.net/10353/d1019852.
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Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
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Clark, M. Sean. "Generalist predators in reduced-tillage corn : predation on armyworm, habitat preferences, and a method to estimate absolute densities /." Thesis, This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-11102009-020213/.
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Dhau, Inos. "Detection, identification, and mapping of maize streak virus and grey leaf spot diseases of maize using different remote sensing techniques." Thesis, University of Limpopo, 2019. http://hdl.handle.net/10386/2866.
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Thesis (PhD. (Geography)) --University of Limpopo, 2019Of late climate change and consequently, the spread of crop diseases has been identified as one of the major threat to crop production and food security in subSaharan Africa. This research, therefore, aims to evaluate the role of in situ hyperspectral and new generation multispectral data in detecting maize crop viral and fungal diseases, that is maize streak virus and grey leaf spot respectively. To accomplish this objective; a comparison of two variable selection techniques (Random Forest’s Forward Variable, (FVS) and Guided Regularized Random Forest: (GRRF) was done in selecting the optimal variables that can be used in detecting maize streak virus disease using in-situ resampled hyperspectral data. The findings indicated that the GRRF model produced high classification accuracy (91.67%) whereas the FVS had a slightly lower accuracy (87.60%) based on Hymap when compared to the AISA. The results have shown that the GRRF algorithm has the potential to select compact feature sub sets, and the accuracy performance is better than that of RF’s variable selection method. Secondly, the utility of remote sensing techniques in detecting the geminivirus infected maize was evaluated in this study based on experiments in Ofcolaco, Tzaneen in South Africa. Specifically, the potential of hyperspectral data in detecting different levels of maize infected by maize streak virus (MSV) was tested based on Guided Regularized Random Forest (GRRF). The findings illustrate the strength of hyperspectral data in detecting different levels of MSV infections. Specifically, the GRRF model was able to identify the optimal bands for detecting different levels of maize streak disease in maize. These bands were allocated at 552 nm, 603 nm, 683 nm, 881 nm, and 2338 nm. This study underscores the potential of using remotely sensed data in the accurate detection of maize crop diseases such as MSV and its severity which is critical in crop monitoring to foster food security, especially in the resource-limited subSaharan Africa. The study then investigated the possibility to upscale the previous findings to space borne sensor. RapidEye data and derived vegetation indices were tested in detecting and mapping the maize streak virus. The results revealed that the use of RapidEye spectral bands in detection and mapping of maize streak virus disease yielded good classification results with an overall accuracy of 82.75%. The inclusion of RapidEye derived vegetation indices improved the classification accuracies by 3.4%. Due to the cost involved in acquiring commercial images, like xviii RapidEye, a freely available Landsat-8 data can offer a new data source that is useful for maize diseases estimation, in environments which have limited resources. This study investigated the use of Landsat 8 and vegetation indices in estimating and predicting maize infected with maize streak virus. Landsat 8 data produced an overall accuracy of 50.32%. The inclusion of vegetation indices computed from Landsat 8 sensor improved the classification accuracies by 1.29%. Overally, the findings of this study provide the necessary insight and motivation to the remote sensing community, particularly in resource-constrained regions, to shift towards embracing various indices obtained from the readily-available and affordable multispectral Landsat-8 OLI sensor. The results of the study show that the mediumresolution multispectral Landsat 8-OLI data set can be used to detect and map maize streak virus disease. This study demonstrates the invaluable potential and strength of applying the readily-available medium-resolution, Landsat-8 OLI data set, with a large swath width (185 km) in precisely detecting and mapping maize streak virus disease. The study then examined the influence of climatic, environmental and remotely sensed variables on the spread of MSV disease on the Ofcolaco maize farms in Tzaneen, South Africa. Environmental and climatic variables were integrated together with Landsat 8 derived vegetation indices to predict the probability of MSV occurrence within the Ofcolaco maize farms in Limpopo, South Africa. Correlation analysis was used to relate vegetation indices, environmental and climatic variables to incidences of maize streak virus disease. The variables used to predict the distribution of MSV were elevation, rainfall, slope, temperature, and vegetation indices. It was found that MSV disease infestation is more likely to occur on low-lying altitudes and areas with high Normalised Difference Vegetation Index (NDVI) located at an altitude ranging of 350 and 450 m.a.s.l. The suitable areas are characterized by temperatures ranging from 24°C to 25°C. The results indicate the potential of integrating Landsat 8 derived vegetation indices, environmental and climatic variables to improve the prediction of areas that are likely to be affected by MSV disease outbreaks in maize fields in semi-arid environments. After realizing the potential of remote sensing in detecting and predicting the occurrence of maize streak virus disease, the study further examined its potential in mapping the most complex disease; Grey Leaf Spot (GLS) in maize fields using WorldView-2, Quickbird, RapidEye, and Sentinel-2 resampled from hyperspectral data. To accomplish this objective, field spectra were acquired from healthy, moderate and xix severely infected maize leaves during the 2013 and 2014 growing seasons. The spectra were then resampled to four sensor spectral resolutions – namely WorldView-2, Quickbird, RapidEye, and Sentinel-2. In each case, the Random Forest algorithm was used to classify the 2013 resampled spectra to represent the three identified disease severity categories. Classification accuracy was evaluated using an independent test dataset obtained during the 2014 growing season. Results showed that Sentinel-2 achieved the highest overall accuracy (84%) and kappa value (0.76), while the WorldView-2, produced slightly lower accuracies. The 608 nm and 705nm were selected as the most valuable bands in detecting the GLS for Worldview 2, and Sentinel-2. Overall, the results imply that opportunities exist for developing operational remote sensing systems for detection of maize disease. Adoption of such remote sensing techniques is particularly valuable for minimizing crop damage, improving yield and ensuring food security.
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Bujold, Isabelle. "The use of a fungal antagonist to reduce the initial inoculum of Gibberella zeae on wheat and corn debris /." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31199.
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Gibberella zeae (anamorph: Fusarium graminearum) is the causal agent of fusarium head blight (FHB) and maize ear rot, two major diseases of wheat and corn in Eastern Canada.In Quebec, Microsphaeropsis sp., an antagonist of Venturia inaequalis, the causal agent of apple scab, was isolated from the apple leaf litter. This fungus, well adapted to Quebec climate, can reduce the initial inoculum of V. inaequalis. FHB and Gibberella ear rot are similar to apple scab because the major inoculum source comes from melanized structures produced on crop residues. Consequently, we evaluated the potential of Microsphaeropsis sp. (isolate P130A) to inhibit ascospore production of G. zeae when applied to crop residues as post harvest or pre-planting applications. Under in vitro conditions, the antagonist significantly reduced ascospore production on wheat and corn residues, when applied prior to (82% and 92% respectively) or at the same time as the pathogen (36% and 58% respectively). Under field conditions, the antagonist had no effect on the pattern of perithecia maturation but significantly reduced the number of ascospores produced on two sampling dates, May 1998 and July 1999. (Abstract shortened by UMI.)
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Odeyemi, Oluwakemi Oluwaseyi. "Studies on the use of essential oils for the control of Sitophilus Zeamais (Motschulsky) (Coleoptera; Curculionidae): a pest of stored maize grains." Thesis, University of Fort Hare, 2008. http://hdl.handle.net/10353/168.
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The common maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), a well known pest of stored-maize grain in most parts of the world, was identified as one of the major constraints of harvested maize grains in the Eastern Cape Province, South Africa. The use of plants or their products is one of the recent methods being investigated for insect pest control worldwide. Therefore, the main aim of the present study was to identify available plants in the Eastern Cape that could be used to combat the problem of Sitophilus zeamais in stored-maize grains. For the realization of the aims of this research, the following studies were carried out; a preliminary survey was conducted to obtain baseline information on the farmers’ knowledge and experience of indigenous insect pest control methods in the Eastern Cape. Also, studies on the insecticidal potential of the essential oils of some plants were investigated against the maize weevil. The quality parameters of maize grains treated with the essential oils was also studied and, using a rat model, the toxicity of the essential oils was investigated. The outcome from this study revealed that there is awareness amongst the farmers in the Eastern Cape on the use of plants or their products to control insect pests. Unfortunately, such methods are currently being neglected and the knowledge of their application was found to be eroding. Among the various essential oils screened were those from Mentha longifolia L. and Tagetes minuta L. which evoked an appreciable level of contact, fumigant and repellent toxicity on the maize weevil. Further work done to determine the effects of the oils on maize stored over a period of three months revealed that the two oils had no adverse effect on the proximate compositions and some quality parameters of the stored maize. However, the toxicological study conducted on rats showed that the oils at tested concentrations exhibited some level of toxicity. It is, therefore, suggested that the essential oils of M. longifolia and T. minuta should not be used to treat maize grains intended for human consumption.
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Oliveira, Dillaine Hennig de. "Ocorrência de fumonisinas em milho e derivados, destinados à alimentação humana." Universidade Tecnológica Federal do Paraná, 2016. http://repositorio.utfpr.edu.br/jspui/handle/1/1876.
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A cultura do milho é um dos segmentos mais importantes para a economia do agronegócio brasileiro, sendo responsável por 37% dos grãos produzidos no país. Entretanto, devido ao seu alto valor nutritivo, possui grande suscetibilidade ao crescimento fúngico, com destaque para Fusarium sp. que além de agente deteriorante é responsável por produzir fumonisinas, um grupo de micotoxinas de caráter termoestável e possivelmente carcinogênico, associada a surtos ocorridos em equinos, suínos e aves. Assim, este trabalho teve como objetivo avaliar a ocorrência de fumonisinas em milho e seus derivados durante o processo de industrialização. Um total de 1200 amostras (300 de milho, 300 de canjica, 300 de grits e 300 de farinha de milho) foram coletadas durante o processamento do milho no mês de maio de 2016 e submetidas a quantificação de fumonisinas pelo método de imunoensaio ROSA® Fumonisin Quantitative, cujo limite de detecção foi de 250 µg/kg. Paralelamente foram coletadas aleatoriamente 23 amostras (9 de milho e 14 de farinha), que foram submetidas simultaneamente a quantificação de fumonisinas pelo método ROSA® e pelo método Cromatografia Líquida de Alta Eficiência, acoplado a espectrometria de massa sequencial LC MS/MS, a fim de estudar a existência de uma correlação entre os métodos. Das 1200 amostras analisadas, 370 (30,83%) apresentaram resultado positivo para fumonisinas totais, com concentrações variando de 278 a 1.522 µg/kg no milho, de 293 a 1.240 µg/kg na canjica, de 269 a 445 µg/kg no grits e de 267 a 512 µg/kg na farinha de milho. Entretanto, os teores de fumonisinas detectados no milho e derivados encontraram-se abaixo dos limites máximos tolerados pela legislação brasileira, sendo portanto, considerados aptos para consumo humano. O método ROSA® utilizado para quantificação de fumonisinas neste trabalho apresentou uma correlação de Pearson positiva forte (r = 0,98) com o método oficialmente empregado, indicando a possibilidade de utilização na quantificação de micotoxinas pelas indústrias processadoras, que necessitam de resultados rápidos, confiáveis e de baixo custo. Contudo, apesar dos níveis de fumonisinas detectados não representarem um risco à saúde dos consumidores, sugere-se um monitoramento constante de produtos à base de milho, tendo em vista que a contaminação por micotoxinas depende de um conjunto de fatores e representa um grande desafio para as indústrias processadoras de milho.The culture of corn is one of the most important segments for the economy of the Brazilian agriculture industry, being responsible for 37 % of the grains produced in the country. Therefore, due to its high nutritious value, it possesses a great vulnerability of fungus growth, especially for the Fusarium sp., which is a deteriorate agent and responsible for producing fumonisin, a group of mycotoxins of carcinogenic character and term stable, associated to outbreaks that reach to equine, suine and poultry and also lead to esophagus cancer in humans. Thus, the aim of this work was to evaluate the incidence of fumonisin in the corn and its derivatives during the industrialization process. A total of 1200 samples (300 maize, 300 of hominy, 300 of grits and 300 of corn flour) was collected among the processing of corn during May 2016 and submitted to fumonisin quantification by method of immunoassay ROSA® Fumonisin Quantitative Method. The detection limit was 250 µg/kg. In order to verify the existence of a Pearson correlation between two different methods for fumonisin quantification, other 23 samples (9 maize and 14 flour) were randomly collected in a parallel study, followed by fumonisin quantification by simultaneously ROSA® Fumonisin Quantitative Method and the Liquid Chromatography-Mass Spectrometry LC-MS/MS. Of the 1200 samples analyzed, 370 (30,83%) tested positive for total fumonisins with concentrations ranging 278-1.522 µg/kg in corn, 293-1.240 µg/kg in hominy, 269-445 µg/kg in grits and 267-512µg/kg in corn flour. However, fumonisin levels detected in corn and derivatives were lower than the maximum level established by Brazilian law, and therefore able for human consumption. The ROSA® method for fumonisin quantitation used in this work showed a strong positive Pearson correlation (r = 0.98) with the official method employed, indicating the possibility of its use in the quantification of mycotoxins by the processing industries, that require fast, reliable and cost-effective results. Despite the detected levels fumonisins did not represent any risks to the consumers health, the corn products monitoring there must be constant, seeing that the contamination through mycotoxins depends on a group of factors and represents a great challenge to the corn manufacturing industries.
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Silva, Adriana Moreira da Silva e. "Estudo dos mecanismos de defesa de plantas de milho atraves das abordagens de analise proteomica e mapeamento de QTLs." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314675.
Full textAbstract:
Orientador: Sergio MarangoniTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: As plantas são capazes de responder e resistir ao ataque de patógenos ativando uma diversidade de estratégias de defesa. A maioria delas exibe uma estratégia geral onde são ativadas respostas bioquímicas de maneira coordenada, incluindo a reprogramação do metabolismo celular, o reforço das barreiras celulares e produção de compostos antimicrobianos e proteínas que agem diretamente sobre o patógeno. Mas existem também respostas específicas das plantas a determinados patógenos, onde são ativadas vias de defesa específicas.Apesar da crescente quantidade de dados em literatura descrevendo genes envolvidos na patogênese vegetal, pouco se sabe sobre as modificações ao nível de proteoma associadas com estas interações. Neste trabalho é apresentado um estudo proteômico comparativo de plantas de milho, de dois genótipos contrastantes em relação à resistência ao fungo Puccinia polysora, em que foram caracterizadas as diferenças no perfil de expressão de proteínas em sementes dos dois genótipos. Através de uma ampla caracterização dos perfis de proteína por diferentes métodos eletroforéticos, foram reveladas 12 proteínas diferencialmente expressas no genótipo de maior resistência. Destas, 5 foram identificadas por espectrometria de massas por MALDI-TOF, sendo 3 delas identificadas como proteínas com atividade de defesa: uma lipoxigenase de 96 kDa, uma proteína Vicilin-like de 66 kDa e uma proteína Heat-Shockde 70 kDa. No segundo capítulo da tese, apresentamos um estudo complementar de mapeamento de QTLs em plantas de milho para a identificação dos genes reguladores da síntese de DIMBOA, um composto secundário que atua na defesa de plantas contra fungos e insetos. Neste estudo foi construído um mapa genético de ligação de uma população F2 resultante do cruzamento de uma linhagem com alta produção de DIMBOA e outra linhagem de baixa produção deste composto. O mapa, com um tamanho total de 1432,9 centimorgans, foi construído com 123 marcadores SSR. Com este mapa foi possível identificar quatro QTLs potencialmente relacionadas com a regulação da via de síntese de DIMBOA, sendo uma no cromossomo 1 (bin 1.08), duas no cromossomo 6 (bin 6.01 e bin 6.02) e uma no cromossomo 10 (bin 10.05)
Abstract: Plants have the ability to respond to invasion by pathogens through activation of a variety of defense strategies. Most plants exhibit a general defense strategy in which a wide range of biochemical responses are induced in a coordinated manner, including reprogramming of cellular metabolism, accumulation of barrier-forming substances and production of antimicrobial compounds and proteins that act directly to prevent pathogen invasion. Some plants show also specific pathogen responses, in a different strategy pathway. Although there is an increasing amount of literature dealing with genes involved in bacterial and fungal pathogenesis, very few reports have addressed proteome modifications associated with such interactions. In the present work we show a comparative proteomic analysis of maize plants, resistant and susceptible genotypes to Puccinia polysora fungi infection and the characterization of differences in protein expression profiles of seeds. Protein profiles of both genotypes were analyzed by a broad range of electrophoresis methods and we could identify 12 proteins differentially expressed in resistant genotype. Five of them were identified by MALDI-TOF mass spectrometry and 3 of them were identified as defense related proteins, a 96 kDa lipoxygenase, 66 kDa Vicilin-like protein and 70 kDa Heat-Shock protein. We also present a complementary study of QTL mapping for identification of regulatory genes controlling DIMBOA synthesis in maize. In this study is shown a linkage map of a F2 population resultant from crossing between two maize lines presenting high DIMBOA production and low DIMBOA production. This map presents 1432,9 centimorgans and was constructed using 123 SSR markers. Using this map we could identify four QTLs possibly related with DIMBOA production, one QTL on bin 1.08 of chromossome 1, two QTLs on bins 6.01 and 6.02 on chromossome 6, and one last QTL on bin 10.05 on chromossome 10
Doutorado
Bioquimica
Doutor em Biologia Funcional e Molecular
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Borin, Regis Callegaro. "Fungicidas associados à fosfitos e complexos nutricionais interferem na qualidade de sementes de milho." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2429.
Full textAbstract:
O milho (Zea mays L.) é um dos cereais mais cultivados no mundo e apresenta papel importante na economia mundial. Com a expansão do plantio da cultura, reduziu-se o convívio harmônico entre a cutlura e as doenças, aumentando com isso, os danos causados, principalmente por fungos fitopatogênicos. Sabendo-se destas necessidades, foram realizados dois estudos que envolveram o uso de fosfitos isolados e, associados com fungicidas comerciais recomendados para o tratamento de sementes. Objetivou-se avaliar o potencial dos fosfitos sobre Fusarium verticilliodes e Fusarium graminearum em condições in vitro e em in vivo sobre a sanidade e qualidade fisiológica das sementes e uma possível ativação do metabolismo de defesa das plantulas. Para o primeiro estudo, foi realizado um experimento in vitro, objetivando avaliar o efeito dos fosfitos, nas doses correspondentes à 0; 0,2; 0,4; 0,6; 0,8; e 1mL/1,0 Kg de sementes. Os fosfitos foram depositados em meio de cultura vertidos em meio BDA, ajustando-se o pH para 5,6. Em placas de Petri, foram dispostos discos de micélio de 7 mm dos fungos F. verticilliodes e F. graminearum. As placas foram incubadas em temperatura de 24 + ou – 1ºC com fotoperíodo de 12 horas luz/escuro. Então, avaliou-se o crescimento micelial em resposta aos tratamentos. Ainda, nesse estudo avaliou-se a sanidade de sementes de milho, tratadas com misturas de fosfitos e fungicidas comerciais. Para o tratamento das sementes, usou-se os fungicidas carbendazim + thiram, fludioxonil + metalaxyl−m em associação com fertilizantes a base de fosfito de potássio, de cobre, complexo nutricional e complexo potencializador, nas doses de 1,5; 3,0; 1; 1; 2 e 3 mL/1 Kg de sementes respectivamente. Para o segundo estudo, os fertilizantes a base de fosfitos de potássio e de cobre foram associados aos fungicidas comerciais carbendazim + thiram, fludioxonil + metalaxyl−m, para avaliar os seus efeitos sobre o desempenho fisiológico das sementes e identificar as possíveis rotas metabólicas envolvidas no processo de defesa vegetal. Foram quantificadas a viabilidade, por meio do teste de germinação e o vigor, submetendo-se as sementes aos testes de envelhecimento acelerado, teste de frio, comprimento e materia seca de plântulas e emergencia em campo, calculando-se o indice de velocidade de emergência, velocidade de emergência e coeficiente de velocidade de emergência. O material vegetal, foi coletado e realizadas as análises bioquímicas de proteínas totais, e atividade das enzimas fenilalanina amônialiase, quitinase e β 1,3 glucanase. O crescimento micelial in vitro dos dois fungos foi afetado pelos tratamentos à base de fosfitos. Ocorreu maior supressão micelial com o fosfito de potássio e fosfito de manganês. Verificou-se que o melhor estado sanitário das sementes foi obtido quando utilizou-se a mistura de carbendazim + thiram e fosfito de potássio. O efeito dos fosfitos de potássio e cobre no tratamento de sementes de milho, associados a fungicidas incrementaram o seu potencial fisiológico. Os tratamentos não interferiram na ativação da rota dos fenilpropanóides e na síntese das enzimas quitinase e β 1,3 glucanase, quando utilizados para o tratamento de sementes de milho.Corn (Zea mays L.) is one of the most cultivated cereals in the world and plays an important role in the world economy. With the expansion of planting of the culture, the harmonic conviviality between the cutlura and the diseases was reduced, increasing with this, the damages caused, mainly by phytopathogenic fungi. Knowing these needs, two studies were carried out that involved the use of isolated phosphites and, associated with commercial fungicides recommended for the treatment of seeds. The objective of this study was to evaluate the potential of phosphites on Fusarium verticilliodes and Fusarium graminearum in in vitro and in vivo conditions on the physiological quality and health of the seeds and a possible activation of the seedling defense metabolism. For the first study, an in vitro experiment was carried out to evaluate the effect of the phosphites at the doses corresponding to 0; 0.2; 0.4; 0.6; 0.8; And 1mL / 1.0 Kg of seeds. The phosphites were deposited in culture medium poured into BDA medium, adjusting the pH to 5.6. In Petri dishes, 7 mm mycelium discs of the fungi F. verticilliodes and F. graminearum were arranged. The plates were incubated at a temperature of 24 ± 1 ° C in a 12-hour light / dark photoperiod. Then, the mycelial growth in response to the treatments was evaluated. Also, in this study, the sanity of maize seeds, treated with mixtures of commercial phosphites and fungicides, was evaluated. For the treatment of the seeds, the fungicides carbendazim + thiram, fludioxonil + metalaxyl-m were used in association with fertilizers based on potassium phosphite, copper, nutritional complex and potentiating complex, at doses of 1,5; 3.0; 1; 1; 2 and 3 mL / 1 Kg of seeds respectively. For the second study, fertilizers based on potassium and copper phosphites were associated with the commercial fungicides carbendazim + thiram, fludioxonil + metalaxyl-m, to evaluate their effects on the physiological performance of the seeds and to identify the possible metabolic routes involved in the Process of plant defense. The viability, through the germination test and the vigor, were quantified by subjecting the seeds to the tests of accelerated aging, cold test, seedling length and dry matter and field emergence, calculating the rate of emergency, emergency speed and emergency speed coefficient. Plant material, was collected and performed the biochemical analyzes of total proteins, and activity of the enzymes phenylalanine ammonia lyase, chitinase and β-1,3 glucanase. In vitro mycelial growth of the two fungi was affected by the phosphite treatments. Mycelial suppression occurred with potassium phosphite and manganese phosphite. It was found that the best sanitary condition of the seeds was obtained when the mixture of carbendazim + thiram and potassium phosphite was used. The effect of potassium and copper phosphites on corn seed treatment associated with fungicides increased their physiological potential. The treatments did not interfere in the activation of the phenylpropanoic route and in the synthesis of the enzymes chitinase and β-1,3 glucanase when used for the treatment of corn seeds.
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Panzo, Josue Domingos. "The incidence of fungi and their mycotoxins in Angolan food and crops with particular reference to maize." Thesis, 2012. http://hdl.handle.net/10210/5344.
Full textAbstract:
M.Tech.Fungi and their mycotoxins are major contaminants of cereals and maize which is the major staple food for Angolan population. The consumption of fungal and mycotoxins contaminated maize can induce health problems as these mycotoxins are known to induce immune suppression and other chronic diseases known as mycotoxicoses. In addition, to these mycotoxins also induce chronic diseases such as cancers of various types (liver, oesophageal, brain). Mycotoxins also immensely affect organs such as fragility and haemorrhage in tissues, liver-necrosis, bile-duct proliferation, caustic effects, intestinal haemorrhage and diarrhoea, adenomatosis, tremors, inco-ordination, mania, coma, photosensitization, sloughing of the extremities, nephrosis, uremia, infertility, prolonged oestrus, huge implications and infections in people with Human immunodeficiency virus (HIV)- AIDS. There is a very little known about the incidence of mycotoxins in Angola, which are associated with Food, Environment and Health. Therefore, the aim of this scientific novel study in history of Angola was to analyse maize samples obtained from major markets in Angola and to establish their quality with regard to mycotoxins and fungi and well as to establish possible links to the prevalent diseases as well as chronic diseases recorded among Angolan populations. To attain these aims, 60 samples of maize were randomly selected and purchased from open rural and nonregulated informal (dusty and food exposed to open air) markets around Luanda, Angola. Luanda, being the capital city of Angola was found to be the best place to sample, because of the presence of huge markets in which food products as well as maize produced in different provinces are delivered and sold to its high population. Thus, sampling in these markets was representative of the whole country and the results obtained give an approximate the status of the quality of maize consumed in different parts of Angola. The result from this study approximates the status of possible health risks that consumers can be exposed due to consumption of these mycotoxins. The samples were analysed in the Food Environmental and Health Research Laboratories at the University of Johannesburg for fungi and mycotoxins contamination using fungal screening methodologies. In terms of mycotoxins extraction, a multi-mycotoxin (solvent-solvent) extraction, solid phase extraction using SAX column for fumonisins (FBs) and immuno-affinity column (VICAM) were used for confirmation of results. Normal thin layer chromatography (TLC), reversed phase thin layer chromatography (RPTLC) (both for FBs confirmation), fluorometer “VICAM” and high performance liquid chromatography (HPLC) were techniques used for mycotoxins detection, identification and quantification. From the results obtained in this study, fungal isolation revealed contamination of the three most important toxigenic species: Aspergillus, Penicillium, Fusarium spp.
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Chilaka, Cynthia Adaku. "A survey of South African commercial feed grade maize for mycotoxins with particular reference to fumonisins using different analytical techniques." Thesis, 2012. http://hdl.handle.net/10210/8057.
Full textAbstract:
M.Tech.Maize (Zea may) is the third most important cereal in the world serving various purpose of economic importance especially as staple diet to the Africans and as a major component of animal feed. Unfortunately, this commodity serves as a suitable substrate for pest and fungi development which may result in the production of mycotoxins. Mycotoxins are secondary metabolites of varying chemical structures produced by filamentous fungi, which may contaminate agricultural commodities either in the field or at storage. Mycotoxins have been implicated to cause several diseases in humans and animals ranging from acute to chronic. This study was designed to determine and quantify the occurrence and levels of mycotoxins in South African feed grade commercial maize. A total of 40 commercial feed grade maize samples were randomly sampled from two factory sites (Factory A and Factory B) of a commercial feed company source from known South African maize producers. The samples were screened for fungi using the conventional method while the mycotoxin screening and quantification was done by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). In addition to TLC and HPLC, VICAM/HPLC, VICAM/fluorometer, enzyme linked immuno-sorbent assay (ELISA) and lateral flow method were used for determination and quantification of fumonisins. The mycological investigation revealed the occurrence of several fungal species of Fusarium, Aspergillus and Penicillium with Fusarium being the most prevalent (100%). Among the Fusarium spp. were F. verticillioides, F. proliferatum, F. oxysporum and F. graminearum, F. subglutinans, F. chlamydosporum, F. solani, F. poae and F. dimerum. Fusarium verticillioides and F. proliferatum had the highest incidence rate of 89% and 73%, respectively, followed by F. oxysporum (65%) and F. graminearum (48%). The rate of occurrence of A. fumigatus, A. flavus and Penicillium spp. were 45%, 43% and 38%, respectively. Further analysis on the isolated fungal strains proved that over 50% of the fungal spp. were toxigenic. Mycotoxicological study on the samples revealed that the samples were contaminated with fumonisins (FB), aflatoxins B (AFB), ochratoxin A (OTA) and zearalenone (ZEA) on TLC. Mycotoxins levels in the samples were confirmed on HPLC, with the levels ranging from 0.064-1.035 ppm for FB, 0-0.762 ppm for iii AFB, 0-0.194 ppm for OTA and 0-0.135 ppm for ZEA. There was no significance difference on mycotoxin concentration in the feed grade sample between the two factory sites. Results obtained from the VICAM methods, ELISA and lateral flow method for FB determination showed 100% incidence rate of the feed grade maize samples similar to that observed on HPLC. Although ELISA (0.190-2.450 ppm) and lateral flow method (0.350-2.700 ppm) showed higher concentration of FB compared to HPLC (0.064-1.035 ppm), recovery analysis on sample using lateral flow gave high value (85%) comparable to HPLC. Further analyses were performed to determine the cytotoxic potential of the feed grade maize samples using the fumonisin fraction (FFraction). The data obtained showed that the feed grade maize extracts were toxic on human peripheral blood mononuclear cells (HPBMC) and the degree of effect on the cells is dependent on the concentration of FB and the duration of exposure. However using oneway ANOVA to determine the effect of different concentration (volumes) of toxin and time of exposure on HPBMC in this study, it was observed that there was no significant difference regardless of the concentration and time.
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Kanyomeka, Luke. "Tolerance of maize genotypes to selected herbicides." Thesis, 2002. http://hdl.handle.net/2263/28633.
Full textAbstract:
Maize damage has been observed from time to time in many parts of South Africa where registered herbicides were applied. Differential cultivar tolerance to certain herbicides was identified as an important factor in many cases where herbicide selectivity was inadequate. A study was done to investigate the existence of differential tolerance of maize genotypes to selected herbicides. Several maize genotypes were screened for tolerance to selected herbicides, both in the greenhouse and in the field. Maize genotypes demonstrated significant differences in their tolerance to all herbicides. Some genotypes were severely injured by certain herbicides while others were not affected at all by the same herbicide. This suggests that maize genotypes should be screened for tolerance to herbicides in order to reduce crop injury by using only appropriate ones for specific genotypes. Generally, hybrids were more tolerant to herbicides than inbreds, indicating that screening may be most necessary in seed production, where inbreds are used, than in commercial production where only hybrids are used. Tolerance of maize to herbicides was more variable to metazachlor than to other herbicides, and metazachlor also injured more genotypes than other herbicides. This was probably due to differences in herbicide mechanism of action. Shoot or root dry mass reduction of some of the maize genotypes occurred without visual injury symptoms, thus suggesting that visual injury may not reliably indicate susceptibility or tolerance to herbicides. The degree of correspondence of herbicide effects on maize in the greenhouse and the field was determined. Comparison of results from the greenhouse and the field showed that there is positive correlation between herbicide effects in the greenhouse and in the field. There was generally good correspondence of major parameters, such as shoot dry mass and injury symptoms, in the greenhouse and in the field. Similarly these parameters were positively correlated with the grain yield obtained from the field. It appears that shoot dry mass and visual injury symptoms could be good predictors of the yield. This indicates that reliable data could be generated through quicker screening at greenhouse level. A total of four herbicides, metazachlor, dimethenamid, acetochlor and the combination atrazine / metolachlor / terbuthylazine, had significant correlations while only two, flufenacet and acetochlor + atrazine/sulcotrione, had no significant correlations for major parameters with the yield. This indicates that the correlation of data was herbicide-dependent. The influence of temperature on maize tolerance to alachlor, metazachlor and metolachlor was investigated. Results showed that low temperatures reduce the tolerance of maize to these herbicides. This could mean that low temperature may reduce the selectivity of these herbicides. Fluctuating temperature conditions of 10°C at night and 35°C during the day, which are found in some maize producing areas, did not affect maize tolerance to the herbicides. The possibility of improving metazachlor tolerance in maize was also investigated. Evidence provided for possible gene effects on the tolerance of metazachlor indicates that maize tolerance to the herbicide could be improved by crossing tolerant parents. The results suggest that it may be possible to improve metazachlor tolerance by crossing appropriate parent lines with dominant genes for tolerance to metazachlor. Ultrastructural changes in the maize seedling root and shoot cells caused by metazachlor were investigated. In susceptible genotypes root cell nucleoli were found to be abnormally large, empty and more abundant than those in untreated control plants. In susceptible plants the chromatids appeared disorganised in cell nucleoli, and both the nuclear and plasma membranes showed signs of disintegrating. There were more and larger vacuoles in the herbicide-susceptible plants. Leaf cells from the susceptible plants had more empty vacuoles and more chloroplasts with generally disorganised content. The bundle sheath chloroplast membranes were dilated in susceptible plants, and the orientation of the grana was disrupted. In the herbicide-tolerant plants, the ultrastructure was not different from that of all the untreated plants. The established differential tolerance of maize to herbicides necessitates the screening of all genotypes to all registered herbicides in order to recommend specific herbicides for certain maize genotypes. Due to the large number of genotypes that would require screening, techniques that yield reliable data quickly have obvious merit. Pot experiments under controlled conditions, which could be selected to promote herbicide bioactivity, are likely to provide data with which the best possible predictions on the risk of herbicide damage in the field could be made. Based on this requirement, environmental factors that should be considered for greenhouse work are: soil with low adsorptive capacity, soil water content close to the field capacity level, and cool temperatures. When screening for herbicide tolerance, the use of herbicide rates in excess of the recommended rate could obviate the need for special environmental conditions, since all the aforementioned factors basically promote the accumulation of higher than usual amounts of herbicide at the site of action in the plant. Therefore, the use of at least a 2X-herbicide rate in screening experiments is advised.Thesis (PhD (Plant Production: Weed Science))--University of Pretoria, 2006.
Plant Production and Soil Science
unrestricted
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Helmich, Bryan T. "Controlling damage by ring-necked pheasants to sprouting corn in southwestern Idaho." Thesis, 1990. http://hdl.handle.net/1957/37928.
Full textAbstract:
During the springs of 1988 1989, experiments were
conducted to determine effective methods of controlling damage
by ring-necked pheasants (Phasianus coichicus) to sprouting
corn in southwestern Idaho. Plot evaluations (1988) failed
to demonstrate the ability of Miller Hot Sauce Animal
Repellent, Isotox Seed Treater (F), provision of alternate
food, and bird scaring reflective tape to reduce damage. When
evaluated using entire production fields for replicates (1989)
firecrackers on fuse rope, bird scaring reflective tape, hawk-kites,
and provision of alternate food all significantly
reduced the number of seedlings disturbed by ring-necked
pheasants. No treatment in either experiment significantly
increased the number of surviving corn seedlings. Fields
were damaged from germination until the average sprout height
exceeded 10 cm, but damage was greatest (69%) when sprouts
were less than 5 cm tall. Management recommendations to avert
damage include establishing treatments prior to the onset of
damage and avoiding early planting dates.Graduation date: 1991
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(9133244), Wyatt Steven Petersen. "Weed Control in Cover Crop No-Till Corn Systems." Thesis, 2020.
Find full textAbstract:
Other field experiments were conducted at the same locations in 2017 through 2018 and 2018 through 2019 to determine the effect of cover crop species, termination timing, and chemical cover crop termination strategies on weed control and corn yield. Crimson cover (Trifolium incarnatum L.), cereal rye, and a cereal rye/crimson clover mix were terminated two weeks before, at, and two weeks after corn planting. All plots were terminated using glyphosate and atrazine, however others were also terminated with dicamba and acetochlor. The addition of acetochlor generally reduced early-season weed biomass or density, but not in cereal rye and cover crop mix treatments that were terminated at or after corn planting. Late-season summer annual grass biomass was reduced when cover crop biomass at termination was over 8000 kg ha-1. Late-season common cocklebur density in 2018 was 450% to 800% higher in cover crops containing cereal rye, compared to crimson clover treatments. Corn yield was reduced by 23% to 67% in cereal rye and cover crop mix treatments in two out of three site-years in 2018, however corn yield was not reduced by crimson clover in either year, nor by cereal rye or the cover crop mix in 2019.
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De, Waele Elsie. "A study of the interaction between Pratylenchus spp. (Nematoda) and Zea mays L." Thesis, 2014. http://hdl.handle.net/10210/12897.
Full text
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Drapek, Raymond James. "Use of a geographical information system to modify pheromone trap-based predictions of Helicoverpa zea (Boddie) damage." Thesis, 1993. http://hdl.handle.net/1957/35398.
Full textAbstract:
The GRASS (V. 4.0) geographical information system (GIS) was used to test
the hypothesis that pheromone trap based predictions of Helicoverpa zea (Boddie)
damage in processed sweet corn could be improved by considering spatial patterns of
corn plantings and wind blocking features around the trap. Wind blocking features
include: tree rows, wooded areas, large buildings close to the trap, and abrupt
hillsides. Pheromone traps were monitored at 29 and 30 sites in 1990 and 1991.
Corn development through the year and damage levels at harvest (percent
infestation) were also recorded for these locations. Maps of all corn plantings and
wind blocking features within 2.5 kilometers of the trap were created, digitized, and
entered into the GIS for each site. A stepwise regression analysis considering 18
spatial and two non-spatial variables resulted in a highly significant (P<0.001)
four variable regression model with an R�� of 0.82. Spatial input variables used in
this model included the total number of hectares of corn within 2.0 kilometers of the
trap as well as the average distance to wind blocking features on the north side of
the trap. The non-spatial variables used were cumulative trap catch and date of first
silk. A dynamic simulation model was also created. This was designed to filter from the trap catch those moths coming from plantings outside of the trapped planting. This model functioned by creating map surfaces in the GIS showing for each day the relative contribution to catch for each location around the trap. This map surface was the product for each location of relative moth population levels and the likeliness that a moth positioned there would be captured by the trap. The modified cumulative trap catch was expected to correlate more strongly to damage levels than the unmodified trap catch, but no run of the model produced a significant improvement in the correlation. In the process of collecting data for this project, circumstantial evidence was obtained which indicated that high synchrony between valley-wide moth catch levels and valley-wide timings of silking in corn could be used as an indicator of high damage years.Graduation date: 1994
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Boudreau, Mark Alan 1958. "Effects of intercropping beans with maize on angular leaf spot and rust of beans." Thesis, 1991. http://hdl.handle.net/1957/36577.
Full textAbstract:
Experiments were performed to determine the nature of
maize influence on bean disease in additive-type
intercrops. Overall effects of intercrops on angular leaf
spot (caused by Phaeoisariopsis griseola) in Kenya
indicated >23% reductions (P<0.05) in area under the
disease progress curve (AUDPC) in two of three season-site
combinations. Fertilization tended to increase disease
(135-205%, P<0.10), but changes in bean density or planting
pattern had no effect. Intercrops reduced temperature and
wind velocity, but increased relative humidity. A 27%
AUDPC reduction (P=0.07) in bean rust (caused by Uromyces
appendiculatus) due to intercropping was observed in Oregon
in 1989 and 1990 in two of three locations.
Mechanisms of maize influence on rust were also
assessed in Oregon. Intercropping, and competition of
maize with beans alone, consistently steepened dispersal
gradients (P<0.10). Interference of maize with dispersal
alone tended to flatten gradients. Spore retention in
plots was increased in mid-season, then decreased late in
the season, due to competition in both years (P<0.05).
Intercropping reduced infection by 96% late in 1989
(P<0.05), probably due to microclimatic influence of maize.
The data from these experiments were used as inputs
for computer simulation to evaluate effects of specific
mechanisms on disease dynamics. Combination of all
mechanisms (= intercrop) reduced AUDPC to 32% of monocrop,
using 1989 data. Infection efficiency reductions, and to a
lesser extent dispersal effects, were responsible for these
changes. Intercrop effects declined as pathogen
multiplication rate (DMFR) increased. No intercrop effect
occurred at any DMFR using 1990 data, although interference
and competition effects of maize alone both increased AUDPC
at low DMFR. Partitioning dispersal effects into those due
to gradient slope changes and spore retention indicate that
the latter accounts almost entirely for disease alteration.Graduation date: 1992
32
Gichuru, Lilian Njeri. "Breeding investigations on utility of maize streak virus resistant germplasm for hybrid development in the tropics." Thesis, 2014. http://hdl.handle.net/10413/10694.
Full textAbstract:
Maize (Zea mays L.) supports millions of livelihoods in sub-Saharan Africa (SSA) in terms of
food and feed. Production of the crop is however limited by several factors, among these, maize
streak virus (MSV) disease. Although extensively studied, MSV remains a serious problem in
SSA due to several challenges in breeding MSV resistant maize varieties. These include
integration of MSV resistant germplasm from different backgrounds, reliance on a few resistant
sources, and genotype x environment interactions. This study was designed to assess the
breeding potential of several MSV resistant lines in hybrid combinations. Understanding
architecture of genetic divergence and background of these genotypes would greatly aid in
breeding high yielding and stable MSV resistant hybrids. Experiments were conducted during
2010 to 2012 seasons in Kenya. Diallel crosses and SSR markers were used to characterize
MSV resistant maize inbred lines from three programs of CIMMYT, KARI and IITA.
In general, this study revealed that MSV is still an important problem in Kenya with high
incidence and severity levels in the farmers’ fields. The levels of MSV resistance in locally
grown hybrids needs to be improved. Farmers challenged breeders to develop new hybrids that
combine early maturing, high yield potential and MSV resistance.
The study was successful in identifying the best eight inbred lines for use in breeding new maize
hybrids with MSV resistance. The nature of gene effects was established for the first time, in
particular the role of epistasis and G x E in conditioning MSV resistance in hybrids. Results
indicate serious implications for previous models that ignored epistasis in studying MSV
resistance in maize. The inbreds Z419, S558, CML509 and Osu23i, displayed high levels of
epistasis for MSV resistance. Unless strong sources of MSV resistance, such as MUL114 and
CML509, are used, breeding resistant hybrids will require parents that carry dominant
resistance genes. The additive-dominance model was adequate to explain northern leaf blight
(NLB) resistance in hybrids, indicating fewer complications in breeding NLB resistant hybrids.
The study also reveals that SSR genetic distance data can be used to predict hybrid
performance, especially when the correct set of markers is used. Many previous studies have
not found any significant relationship between genetic distance and heterosis, due to large
G x E and use of a wrong set of markers. The diallel analysis and SSR data established the
important heterotic groups, which will be exploited for efficient development of MSV resistant
maize hybrids. These strategies will be recommended to programs that emphasize MSV
resistance in maize hybrids.Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.
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Mafu, Nothando Fowiza. "Marker-assisted selection for maize streak virus resistance and concomitant conventional selection for Downy Mildew resistance in a maize population." Thesis, 2013. http://hdl.handle.net/10413/10023.
Full textAbstract:
Maize streak virus (MSV) disease, transmitted by leafhoppers (Cicadulina mbila, Naude), and maize downy mildew (DM) disease caused by Peronosclerospora sorghi (Weston and Uppal) Shaw, are major contributing factors to low maize yields in Africa. These two diseases threaten maize production in Mozambique, thus the importance of breeding Mozambican maize varieties that carry resistance to these diseases. Marker-assisted selection (MAS) was employed to pyramid MSV and DM disease resistant genes into a single genetic background through simultaneous selection. Firstly, it was essential to determine the genetic diversity of MSV disease resistance in 25 elite maize inbred lines to aid in the selection of suitable lines for the introgression of the msv1 gene; and subsequently, to introduce the msv1 resistance gene cluster from two inbred lines, CM505 and CML509, which were identified as the ideal parental lines for the introgression of MSV disease resistance into a locally adapted Mozambican inbred line LP23 that had DM background resistance. Pyramiding the resistance genes by the use of simple sequence repeat (SSR) molecular markers to track the MSV gene cluster was investigated in 118 F3 progeny derived from crosses of CML505 x LP23 and CML509 x LP23. High resolution melt (HRM) analysis using the markers umc2228 and bnlg1811 detected 29 MSV resistant lines. At the International Maize and Wheat Improvement Centre (CIMMYT) in Zimbabwe, MSV disease expression of the 118 F3 progeny lines was assessed under artificial inoculation conditions with viruliferous leafhoppers and the effect of the MSV disease on plant height was measured. Thirty-seven family lines exhibited MSV and DM (DM incidence ≤50) disease resistance. Individual plants from a total of 41 progeny lines, that exhibited MSV disease severity ratings of 2.5 or less in both locations within each of the F3 family lines, were selected based on the presence of the msv1 gene based on SSR data, or field DM disease resistance, and were then advanced to the F4 generation to be fixed for use to improve maize hybrids in Mozambique for MSV resistance. Simultaneous trials were run at Chokwe Research Station in Mozambique for MSV and DM disease assessment, under natural and artificial disease infestation, respectively. Thus the MSV and DM genes were effectively pyramided. Lines with both MSV and DM resistance were advanced to the F4 generation and will be fixed for use to improve maize hybrids in Mozambique for MSV and DM resistance, which will have positive implications on food security in Mozambique. This research discusses the results of combined selection with both artificial inoculation and the three selected SSR markers. It was concluded that a conventional maize breeder can successfully use molecular markers to improve selection intensity and maximise genetic gain.Thesis (M.Sc.Agric)-University of KwaZulu-Natal, Pietermaritzburg, 2013.
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Mengesha, Wende Abera. "Genetic diversity, stability, and combining ability of maize genotypes for grain yield and resistance to NCLB in the mid-altitude sub-humid agro ecologies of Ethiopia." Thesis, 2013. http://hdl.handle.net/10413/10935.
Full textAbstract:
Maize (Zea mays L.) is the third most important cereal crops in the world after wheat
and rice. In Ethiopia, maize remains the second largest food security crop after tef
[Eragrostis tef (Zucc.) Trotter.]. The mid-altitude, sub-humid agro-ecology (1000 to 1800
m above sea level) is the most important maize producing environment in Ethiopia.
However, productivity of maize is low, due to several biotic and abiotic constraints.
Among the biotic constraints, Turcicum leaf blight disease of maize caused by
Exserohilum turcicum Pass Leonard & Suggs shows high incidence of 95-100% and
inflicts significant grain losses in the country. Therefore, high yielding, Turcicum leaf
blight resistant and farmers-preferred maize varieties and their production technologies
should be developed and made available to growers to enhance maize production and
to achieve food security.
The objectives of this study were to: (1) assess farmer’s preferences, and production
constraints for maize in the mid-altitude, sub-humid agro-ecology of western Ethiopia,
(2) determine the genetic variability among elite maize inbred lines and select promising
parents for resistance to E. turcicum, (3) determine diversity among the elite germplasm
lines using SSR markers, (4) determine combining ability and heterosis among elite
maize inbred lines and their hybrids, and (5) investigate genotype x environment
interaction and yield stability of experimental maize hybrids developed for the midaltitude
sub-humid agro-ecology of Ethiopia.
A participatory rural appraisal (PRA) research was conducted involving 240 maize
farmers in three representative maize growing zones of western Ethiopia; West Shoa,
East Wollega and West Wollega, each represented by two districts and two subdistricts.
Maize was ranked number one both as food and cash crop by 82.9% of
respondents. Turcicum leaf blight was ranked as number one devastating leaf disease
by 46% of respondents. Breeding for improved disease resistance and grain yield,
enhancing the availability of crop input and stabilizing market price during harvest time
were recommended as the most important strategies to increase maize production by
small-scale farmers in western Ethiopia. Fifty inbred lines were evaluated for reaction to Turcicum leaf blight during the main
cropping seasons of 2011 and 2012. Inbred lines were clustered into resistant
(CML202, 144-7b, 136-a, 139-5j, 30H83-7-1, ILOO’E-1-9, SZYNA-99-F2, and 142-1-e),
and susceptible (CML197, CML464, A7033 , Kuleni C1-101-1-1, CML443, SC22-430
(63), (DRB-F2-60-1-2) – B-1-B-B-B, Pool9A-4-4-1-1-1). Inbred lines (CML312, CML445,
Gibe-1-158-1-1-1-1, CML395, and 124-b (113)) had intermediate response to the
disease. Overall, inbred lines such as CML202, 30H83-7-1, ILOO’E-1-9-1, CML312,
CML395 CML445 and 142-1-e were selected with better agronomic performance and
resistance to leaf blight for breeding. Twenty selected elite parental inbred lines were
genotyped with 20 polymorphic SSR markers. The genotypes used were clustered into
five groups consistent with the known pedigrees. The greatest genetic distance was
identified between the clusters of lines CML-202 and Gibe-1-91.
Eighteen selected inbred lines were crossed using the factorial mating scheme and 81
hybrids developed to determine combining ability effects and heterosis. Inbred lines with
high GCA effect (CML 202, CML395, 124-b (113), ILOO’E-1-9 and CML 197) were
selected as best combiners for hybrid development. Additionally five high yielding novel
single cross hybrids with grain yield of > 8 t ha-1 and high SCA effects were identified
such as CML395 X CML442, DE-78-Z-126-3-2-2-1-1 X CML442, ILOO’E-1-9-1-1-1-1-1
X CML312, X1264DW-1-2-2-2-2 X CML464 and SC22 X Gibe-1-91-1-1-1-1. These
experimental hybrids are recommended for direct production or as hybrid testers for
hybrid development.
Genotype x environment interaction (GEI) effects of 81 newly developed and three
check maize hybrids were evaluated across 10 locations in the mid-altitude sub-humid
agro-ecologies of Ethiopia. The AMMI-3 and GGE biplot models were used to determine
stability. Hybrids such as G68, G39, G37, G77, G34 and G2 were identified as the most
stable and high yielding at favorable environments such as Bako, Jima, Arsi Negelle
and Pawe in Ethiopia. The genotype and genotype by environment interaction (GGE)
biplot clustered the 10 environments into three unique mega-environments. Environment I included Bako, Jima, Asossa, Ambo, Finote Selam, Haramaya and Pawe
while environment II represented by Arsi-Negelle and environment III Areka and
Hawassa.
In general, the study identified valuable maize inbred lines with high combining ability
for breeding and novel single cross hybrids for large-scale production or as testers for
hybrid development at the mid-altitude, sub-humid agro-ecologies of Ethiopia or similar
environments in sub-Saharan Africa.Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.
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(5929796), Joseph T. Ikley. "The Role of Alternative Hosts and Herbicides in the Management of Clavibacter nebraskensis, Causal Agent of Goss’s wilt of Corn." Thesis, 2019.
Find full textAbstract:
The reemergence of Goss’s wilt of corn in the western Corn Belt in 2006, along with subsequent identification of the disease in 16 states, has led to renewed interest in the disease and its epidemiology. Goss’s wilt, caused by the bacterium Clavibacter nebraskensis, is currently the third-leading cause of yield loss in corn from diseases in the United States. Its impact is exacerbated by the fact that cultural control methods are the only current means for its control. The objectives of our research were to (1) determine the role that alternative hosts of the bacterium play in the disease cycle and epidemiology of Goss’s wilt, and (2) determine if postemergence herbicide use affects disease severity. Through a greenhouse experiment, we discovered three new weedy alternative hosts of the disease. In a series of field and greenhouse experiments, we found that C. nebraskensis can overwinter on alternative host and corn debris in Indiana. We found that C. nebraskensis did not become seed-borne in alternative hosts. In contrast to corn, no systemic infections were observed on alternative hosts, with the bacterium being restricted to inoculated leaf tissue. Using herbicides to control C. nebraskensis-infected weeds did not reduce the pathogenicity of the bacterium recovered from treated plants. The use of nicosulfuron, dicamba plus diflufenzopyr, and a 2X rate of glyphosate postemergence increased disease severity in one experiment, but postemergence herbicides did not influence disease severity in a second experiment. Corn yield was not affected. This indicates that herbicide use may play a role in the epidemiology of Goss’s wilt in some years, but ultimately corn yield is not affected. Our results demonstrate that the host range of C. nebraskensis is wider than previously thought, and that postemergence control of alternative hosts may not be sufficient in reducing inoculum levels. Our results suggest that failure to control alternative hosts could negate some of the benefits of crop rotation to reduce inoculum levels in a field, thus playing an important role in the epidemiology of Goss’s wilt.
36
Hoinacki, Elisabeth V. "Sweet corn decline syndrome in Oregon's Willamette Valley." Thesis, 2003. http://hdl.handle.net/1957/29744.
Full text
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Mwimali, Murenga Geoffrey. "Genetic analysis and response to selection for resistance to two stem borers, Busseola fusca and Chilo partellus, in tropical maize germplasm." Thesis, 2014. http://hdl.handle.net/10413/10943.
Full textAbstract:
Maize is the principal staple food in sub-Saharan Africa (SSA), but production lags behind population growth. The African stem borer, Busseola fusca, Fuller (Lepidoptera, Noctuidae), and the spotted stem borer, Chilo partellus, Swinhoe (Lepidoptera, Crambidae) are serious insect pests of maize in tropical environments. The damage can be managed by breeding stem borer resistant maize varieties but there is limited information that can be used to devise appropriate breeding programs. Therefore breeding investigations were conducted to appraise germplasm screening methods, and to determine combining ability, heterosis and response of maize populations to S1 progeny recurrent selection. The study was conducted in Kenya during 2010 to 2013.
The results showed that most of the test genotypes were susceptible to B. fusca and less so to C. partellus, indicating that breeding for B. fusca would be more challenging. Therefore more resources would be required to improve maize germplasm for resistance to B. fusca to broaden the base from which breeders will select suitable lines for breeding. There was a highly significant (r=0.947, p≤ 0.01) correlation between rank selection index in the greenhouse and laboratory. The detached leaf disk bioassay method was effective for screening maize genotypes for resistance to both stem borers. Therefore it will be recommended for use in screening maize genotypes in future studies. The line x tester studies indicated a preponderance of the additive gene effects for borer resistance traits. Specific combining ability effects were significant for resistance traits and grain yield indicating that non-additive effects were also influential. Findings from the breeding investigations will impact positively on both food security and plant breeding capacity. The completed study was successful in identifying new maize inbred lines with resistance to both stem borers. These lines have high utility to maize breeding programmes that emphasise stem borer resistance in tropical environments. For the hybrid-oriented programmes, combining ability and heterotic orientation data for the 66 maize inbred lines will be crucial. In this regard the study was very successful in classifying the lines into three heterotic groups according to single cross testers (CML395/CML444, and CML312/CML442) that are widely used at CIMMYT, and by public breeding programs throughout SSA. Importantly, this was done based on grain yield potential of hybrids under B. fusca and C. partellus infestations in three mega environments.
The study demonstrates that S1 progeny recurrent selection is effective for improving stem borer resistance, without compromising yield. There was significant reduction (69%) in maize plant damage by both pests, and yield gains of 25% to 70% were realised in two populations. This represents significant contribution to plant breeding capacity, especially to maize breeding programmes that emphasise stem borer resistance in hybrids.Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2014.
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Kasozi, Lwanga Charles. "Genetic analysis and selection for maize weevil resistance in maize." Thesis, 2013. http://hdl.handle.net/10413/10746.
Full textAbstract:
The maize weevil (Sitophilus zeamais Motschulsky) is one of the most destructive storage insect pest of maize (Zea mays L.) in tropical Africa and worldwide, especially when susceptible varieties are grown. Therefore, grain resistance against the maize weevil should be part of a major component of an integrated maize weevil management strategy. The specific objectives of this study were to: i) determine farmers’ perceptions about weevil resistance in maize cultivars; ii) determine the genotypic variation for maize weevil resistance in eastern and southern Africa maize germplasm lines; iii) study the gene action conditioning weevil resistance in the inbred line populations from eastern and southern Africa maize germplasm and to measure their combining ability for yield and weevil resistance; iv) determine the effectiveness of two cycles of modified S1 recurrent selection in improving a tropical maize population “Longe5” for weevil resistance and agronomic superiority and v) evaluate the effectiveness of the “weevil warehouse techniques” compared to the “laboratory bioassay technique” as methods of maize screening against the maize weevil.
A participatory rural appraisal (PRA) was conducted in three districts between December 2010 and January 2011, to gather information on the maize weevil pest status in Uganda and farmers’ perceptions about improved maize varieties and the major attributes desired in new maize varieties. Over 95% of farmers knew the maize weevil and its pest status, and were reportedly controlling the maize weevil using wood ashes, red pepper and Cupressus sempervirens. The estimated postharvest weight losses attributed to weevil damage was over 20% within a storage period of four months. The most highly ranked attributes desired in the new maize varieties included high grain yield, tolerance to drought and low nitrogen stresses, resistance to field pests and diseases, good storability and resistance to storage pests.
In the search for new sources of weevil resistance, a total of 180 inbred lines from three different geographical areas were screened for weevil resistance using the laboratory bioassay technique. Eight inbred lines (MV21, MV23, MV75, MV102, MV142, MV154, MV157, and MV170) were consistently grouped in the resistant class, and therefore selected as potential donors for weevil resistance in the maize improvement programs. Large significant genetic variations for weevil resistance, and high levels of heritability (89 – 96%) were observed. The results revealed that there was no significant association between maize weevil resistance andgrain yield; suggesting that breeding for maize weevil resistance can be achieved without compromising grain yield.
Eight weevil resistant and two susceptible inbred line parents were crossed in a 10 x 10 full diallel mating design and the resulting 45 experimental hybrids and their reciprocal crosses evaluated for grain yield and secondary traits under four environments, and also to determine the gene action regulating their expression. The F1 hybrid seed, F2 full-sib and F2 half-sib grain generated from the 45 experimental hybrids and their reciprocals under two environments in Namulonge, were evaluated for weevil resistance using F1 weevil progeny emergence, median development period (MDP), Dobie’s index of susceptibility (DIS), and parental weevil mortality as susceptibility parameters. The general combing ability (GCA), specific combining ability (SCA), and reciprocal effects were all significant for grain yield, with SCA accounting for over 80% of the hybrid sum of squares. Inbred line parent MV44 exhibited positive significant GCA for grain yield and thus can be utilized in the development of synthetics and hybrids. Hybrids MV21 x MV13, MV154 x MV44, and MV154 x MV102 and all hybrids between parent MV142 and the rest of the parental lines exhibited positive and significant SCA effects. For the weevil resistance parameters, the general combining ability (GCA), specific combining ability (SCA) and reciprocal effects were all significant for F1 weevil progeny emergence, MDP, and DIS in the three seed categories. The results revealed that weevil resistance was governed by additive gene action, non-additive, and maternal effects. Parents MV170 and MV142 were consistently exhibiting weevil resistance in the three seed categories and thus recommended for future breeding strategies. Furthermore, most of the hybrids generated from parental line M142 were noted to exhibit outstanding performance in terms of grain yield and weevil resistance.
Another study was conducted to determine the effectiveness of two cycles of modified S1 recurrent selection towards the improvement of weevil resistance in a maize population Longe5. Over 540 selfed ears were selected from the source population (C0) and screened for weevil resistance in the laboratory at Namulonge. Based on weevil resistance characteristics, 162 genotypes were selected from C0 and recombined in an isolated field to generate cycle C1. The same procedure was used for generating cycle C2 from cycle C1, but instead 190 weevil resistant C1 genotypes were selected and recombined to form C2. Seed from cycles C1 and C2, together with that from the source population (C0), was used to plant an evaluation trial in three locations, to compare the performance of the three cycles in terms of grain yield and reaction to the major foliar diseases, and also to produce seed for subsequent screening against weevil ii
infestation. A total of 54 seed samples were screened for weevil resistance in a laboratory at Namulonge, in an experiment laid out in a randomized complete block design. A reduction in grain weight loss of 65% was registered in the C2 seed, whereas in C1 seed it was 15%. A similar trend was observed for F1 weevil progeny emergence and grain damage. Grain yield results indicated a yield gain of 19% realized from cycle C2 while a yield gain of 7% was realized from cycle C1. Furthermore, reductions in disease severity of 27%, 10% and 13% were exhibited for Turcicum leaf blight (TLB), grey leaf spot (GLS) and rust disease, respectively in cycle C2. The results indicated that Longe5 can be improved for maize weevil resistance, grain yield, and resistance to foliar diseases through selection. Further recurrent selection cycles would be recommended.
The last study was aimed at evaluating the potential of shelled grain and suspended ear options of the weevil warehouse technique in discriminating maize genotypes into different susceptibility classes, based on genotype response to weevil attack. It involved comparing the effectiveness of the two options under the weevil warehouse technique with the laboratory bioassay technique using grain damage and grain weight loss as the maize grain susceptibility parameters. Fourteen maize genotypes were screened using the weevil warehouse and the laboratory bioassay techniques at Namulonge. On grouping the 14 genotypes into different response classes, high levels of consistency were observed in the three screening techniques. Therefore, the two weevil warehouse screening options being faster and effective in discriminating maize cultivars towards weevil attack, they were found to be better than the laboratory bioassay technique. The minimum evaluation period required to discriminate genotypes by the two weevil warehouse options was two months from the onset of the experiment.
The maize weevil was noted to be an important storage pest constraining maize production in Uganda. The major weevil control measures included proper postharvest handling procedures and use of indigenous technical knowledge. The results also revealed that host plant resistance could significantly reduce grain damage. It was further revealed that grain resistance against the maize weevil could be enhanced through hybridization and recurrent selection; thus the germplasm identified in the study can provide new sources of maize weevil resistance for commercial deployment and further breeding.Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.
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Gerber, Johan 1961. "Yield response of Fusarium infected maize seed treated with biological control agent formulations." Diss., 2010. http://hdl.handle.net/10500/4713.
Full textAbstract:
Potential vegetative and reproductive increases in yield, as well as the biological efficacy against Fusarium verticillioides and F. proliferatum causing ear and stem rot in maize crops of commercially-formulated micro-organism formulation T-Gro (Trichoderma harzianum isolate DB103 WP) combined with Spartacus (Beauveria bassiana isolate DB 105 WP), T-Gro combined with Armenius (Bacillus subtilis isolate DB 109 WP), T-Gro combined with Maximus (Bacillus subtilis isolate DB 108 WP), T-Gro combined with Shelter (Bacillus subtilis isolate DB 101), T-Gro combined with Bismarck (Microbacterium maritypicum isolate DB 107 WP), as well as individual treatments of T-Gro, Armenius, Bismarck, Maximus and Shelter, were investigated when applied to maize seed and soil under field conditions. All the micro-organism treatments were compared with Thiram 750WP (750g/kg thiram WP) and an untreated control.
The micro-organism treatments showed an increase in vegetative as well as reproductive yields when compared to the reference product Thiram 750 WP and the untreated control. There were no observations of adverse effects on the germination of maize seed in all the treatments that were applied. The three isolates B. subtilis, T. harzianum, and M. maritypicum, showed a significant reduction in vascular tissue discolouration of the main and ear stems, indicating a potential to be used in the reduction and control of diseases caused by Fusarium spp. Results also showed poor to very good increases of stem and foliage biomass as well as cob yield per plant produced by the micro-organism treatments when compared to the untreated control. The highest cob yield per plant that differed significantly from the untreated control was produced by T-Gro and Shelter.
No phytotoxicity of any kind was observed with the application of the micro-organism formulations and they could therefore be deemed suitable to be used for the treatment of maize seed. The micro-organism formulations containing fungal and bacterial biological control agents have the potential to be used in commercial maize production to increase vegetative and reproductive yields and reduce the severity of ear and stem rot in maize.Agriculture Animal Health & Human Ecology
M.Sc. (Agriculture)
40
Melia-Hancock, Susan. "Effect of European and southwestern corn borers on translocation of photosynthetic products, water use and yield in Zea mays L." 1985. http://hdl.handle.net/2097/27500.
Full text
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Mariote, David. "Response to selection for downy mildew (Peronosclerospora sorghi) and maize streak virus resistance in three quality protein maize populations in Mozambique." Thesis, 2007. http://hdl.handle.net/10413/748.
Full text
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Bucheyeki, Tulole Lugendo. "Characterization and genetic analysis of maize germplasm for resistance to northern corn leaf blight disease in Tanzania." Thesis, 2012. http://hdl.handle.net/10413/8730.
Full textAbstract:
The majority of farmers in Tanzania have not yet adopted modern maize varieties and still
cultivate landraces and open pollinated varieties (OPVs) with low production potential and
susceptible to diseases like maize streak virus (MSV), grey leaf spot (GLS) and northern corn
leaf blight (NLB). The NLB disease is among the major causes of low yield and has been
reported in all 21 maize growing regions in Tanzania. Breeding for host plant resistance with
high yielding potential and involving the community in the breeding process is expected to
address the problem of low yield, NLB disease susceptibility and low rate of F1 hybrid adoption.
Therefore, the study was conducted to obtain additional sources of resistance to NLB disease,
high yielding cultivars with community acceptable traits adapted to Tanzanian conditions. The
main objective was to contribute to increased maize productivity in the western zone of
Tanzania. The specific objectives of this study were therefore to : 1) investigate maize
production limiting factors for smallholder farmers in western Tanzania, 2) identify farmers and
stockist perceptions, opinions and maize variety selection criteria in western Tanzania, 3)
establish NLB disease status in farmers’ fields of western Tanzania, 4) determine the genetic
relationships among landraces and assess maize landraces as sources of breeding materials,
5) determine the combining ability and heterosis for NLB disease resistance of eleven maize
inbred lines adapted to Tanzanian conditions, and 6) determine the gene action and inheritance
of resistance to NLB disease in five maize inbred lines adapted to Tanzanian conditions. The
study was conducted from 2008-2011 in three diverse environments which represent all the
maize growing regions in the country
The participatory rural appraisal (PRA) was conducted in three districts to investigate farmers’
and stockists preferred traits for maize selection in western Tanzania, determine maize
production constraints facing farmers and assess NLB disease prevalence in the same area. A
focus group of 30 farmers was selected in each of the three villages. Transect walks, wealth
ranking and historical profiles were used in an informal survey. One hundred and fifty
questionnaires were used in a formal survey. The recorded yield was only 1 t haˉ¹. Thirteen
major maize production constraints, 13 insect pests and vermin and, 11 diseases were
recorded. The NLB disease was reported to be increasing in severity in all farmers’ fields.
Farmers’ preferred traits included resistance to abiotic and biotic stresses, early maturity,
preferred milling qualities, high storage qualities and high yielding potential. Stockists mentioned
12 preferred maize variety traits which included high yielding, disease and insect pest
resistance, heavy grain, large cob size and large grain sizes. Similarity between farmers and
stockist variety preference ranking were found to exist.
The occurrence and distribution of northern leaf blight (NLB) disease study was conducted to
assess the incidence and severity of NLB disease in farmers’ fields in seven districts. The study
was conducted for two seasons. In each season, 175 fields with 5600 plants were sampled.
There were sixteen varieties grown with wide NLB disease reaction variation. Gembe, a
landrace, was among the three observed resistant varieties. The NLB disease has changed its
distribution pattern affecting all districts of the western zone. The disease incidence in season
two (2009/2010) significantly increased from season one (2008/2009) t= -3.25 (348), P= 0.001.
About 30% of both means of blight incidence and severity were recorded in the area.
Characterization and screening of maize landraces for northern leaf blight disease resistance was
conducted to determine the genetic relationships among landraces, assess maize landraces as
sources of NLB disease resistance and assess important agronomic traits for future maize
improvement. Ninety breeding materials consisting of 71 landraces and 19 commercial varieties
were evaluated. The average yield of landraces under research management was 2.3 t haˉ¹.
Landrace TZA 3075 was identified as NLB disease resistant. Yield potential, dent grain texture,
white endosperm and husk cover were important agronomic traits observed among landraces.
There were high variations in terms of morphology and NLB disease resistance among the
landraces. Five principal components contributed to 71.98 % of total variation. Clusters analysis
revealed five distinct groups of landraces. Leaves/plant, infested leaves/plant, lesion number,
lesion length, lesion width and NLB disease incidence traits highly contributed to variation and
grouping of landraces.
Combining ability analysis for northern leaf blight disease resistance was conducted to estimate
the combining ability for NLB disease resistance of 11 maize inbred lines adapted to Tanzanian
conditions, determine maternal effects which are involved in NLB disease resistance in maize
germplasm, and determine the heterosis in the F1 hybrids. A full 11 x 11 diallel cross was
performed. All top ten experimental hybrids in each of the three sites had negative midparent
heterosis for NLB disease severity. The overall mid-parent heterosis means for yield across
sites was 152%. The mean sum of squares for GCA was highly significant (P< 0.001) on
disease severity indicating additive gene action effects. Mean sum of squares for SCA were
highly significant for disease severity and yield implying non-additive gene action effects.
The mean squares for reciprocal effects were highly significant on yield and non-maternal sum
of squares had significant effect (P<0.05) on yield. The GCA contribution was high for disease
severity (91%) and lesion number (85%). Almost, all GCA effects for NLB disease resistance
were negative implying contribution to disease resistance. Due to preponderance of the additive
gene action, recurrent selection could be used to improve the resistance of inbred lines while
the non-additive gene action could be exploited in breeding for disease resistant hybrids.
Generation mean analysis of northern leaf blight disease resistance was conducted to
determine the mode of gene action involved in the inheritance of resistance to NLB disease in
five inbred lines adapted to Tanzania at contrasting environments, estimate heterosis and
heritability in five tropical inbred lines. Generation mean analysis was conducted using a six
parameter model comprising P1, P2, F1, F2, BCP1 and BCP2 generation progenies. The mean
sum of squares for environment, replication with the nested environment, generations,
generations x environment interactions were highly significant (P<0.001). The full model of
additive, dominance, additive x additive and additive x dominance epistatic effects was highly
significant (P<0.001). Nonetheless, the additive gene effects were predominant ranging
between 57% and 89% which was matched by large heritability (54%-85%). The average
degree of dominance ranged between -0.52 and 0.88 supporting observations of partial
dominance. The NLB disease severity showed a continuous distribution in all three sets for F2,
BCP1 and BCP2 populations which is an indication of quantitative nature of inheritance and
additive gene effects. The mid parent heterosis ranged from -19 to 1%. Therefore, resistance to
NLB disease could be improved through selection by exploiting the additive gene effects. The
epistatic gene effects would cause less complications because they were negligible (<25%).
The client oriented breeding for maize northern leaf blight disease resistance was carried out to
perform farmers and stockists assessment on the 110 F1 experimental maize hybrids and
compare them with breeders selection criteria. Breeders selection criteria ranked 10 top high
yielding experimental hybrids. Farmers developed 14 while stockists developed 13 selection
criteria. The most preferred hybrids by farmers were VL 05616 x CML 159, CML 159 x KS03-
0B15-47 and EB04-0A01-304 x CML 442 while stockists preferred VL 05616 x CML 395,
EB04-0A01-304 x CML 442 and VL 05616 x CML 159. Two F1 experimental hybrids EB04-
0A01-304 x CML 442 and CML 159 x CML 442 appeared in all top five ranked hybrids by
breeders, farmers and stockists. Generally, findings showed that, farmers, stockists and
breeders coincide in some selection criteria but also differ in other cases.Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.
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Kwena, Philip Onyimbo. "Recurrent selection for gray leaf spot (GLS) and phaeosphaeria leaf spot (PLS) resistance in four maize populations and heterotic classification of maize germplasm from western Kenya." Thesis, 2007. http://hdl.handle.net/10413/747.
Full text
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Moodley, Odeshnee. "Effectiveness of varied refugia configurations for genetically modified maize (Zea mays L.) in KwaZulu-Natal midlands." Diss., 2013. http://hdl.handle.net/10500/18319.
Full textAbstract:
Genetically modified (GM) white and yellow maize, Zea mays, has been commercially released and cultivated in South Africa since 1997/1998. The traits expressed are insect resistance and herbicide tolerance conferred by the bacteria Bacillus thuringiensis (Bt) Cry genes and Agrobacterium 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase gene, respectively. The Cry genes have been used widely to control lepidopteran insect pests but insect resistance to GM Bt crops has been a concern since the introduction of this technology. A management strategy includes refugia planting of 5% non-Bt plants, with no insecticide application, and 20%, where insecticide application is allowed. These refugia are designed to allow the survival of insect pests within restricted planted zones. However, in South Africa there are reports of Bt-resistant stem borer (Busseola fusca) (Fuller) (Lepidoptera: Noctuidae) and non-compliance with refuge planting. The aims of this study were two-fold: 1. To conduct a survey among KwaZulu-Natal (KZN) GM maize growers to ascertain information such as level of compliance with refuge planting and to determine which refugia were predominantly planted and reasons thereof; 2. To conduct a replicated field trial to determine yield, insect borer damage and economic benefit of the 5% unsprayed and 20% sprayed refuge options (including three configurations namely strip, perimeter and block and a 5 and 20% ‘refuge-in-a-bag’ option). The survey indicated that 28 out of 29 (96.6%) KZN Bt maize growers plant the 5% non-sprayed refuge with 27 (96.4%) of those respondents planting the strip configuration for the purpose of insect management (75%) and ease of planting (32.2%). The survey also showed that 7 (seven) i.e. 21.9% of KZN Bt maize growers observed borer damage and although growers are now fully compliant with refugia planting requirements, initially 7 respondents (24.1%) did not comply with or plant refugia correctly. Furthermore, 7 respondents reported insect borer damage in their maize with 4 of the 7 instances (57.1%) likely stemming from incorrectly planted refugia.
vii
No significant differences in yield or insect damage were observed between the 5 and 20% refugia for any of the planting configurations in the field trial. However due to costs involved with insecticide application and labour required for the operation in the 20% option, these treatments were less economically advantageous than the non-Bt control. The 20% block and strip configurations had a cost benefit ratio of ZAR 7.21 and ZAR 6.67 respectively, earned per R1 spent by the grower compared with ZAR 7.76 in the sprayed control. The cost-benefit comparison for the 5% block and strip configurations was ZAR 8.48 and ZAR 7.71, respectively compared with ZAR 9.44 in the unsprayed control. In addition, the 20% seed mixture limited borer damage to 4.95% when compared with 15.77% damage in the sprayed control (ANOVA, F pr = 0.124). The seed mixtures are not available commercially and the results from the survey indicated that some education and marketing by the seed companies would be advisable prior to their release to the farming community.
In order to determine which of the refuge options between 5 and 20% would be more advantageous for growers overall, regardless of the planting configuration; data were grouped and analysed. There were no significant differences in either the yield or insect damage for the 5 and 20% refugia, but the cost-benefit calculations indicated that the 5% option was more cost effective – for the 5 and 20% refugia, ZAR 7.97 and ZAR 7.15 respectively, earned per ZAR 1 spent by the grower (ANOVA, F pr. = 0.03). This is because no insecticide was used in the 5% treatments. Mean ear damage comparisons between the 5 and 20% refugia showed that the 20% refuge in the perimeter configuration incurred the least damage (2.65% ear damage) compared with 5% perimeter (10.86% ear damage), although the reasons for this are not clear.
While the results of the field trials showed no significant differences in insect damage and yield with regard to choice of refuge configuration, monitoring insect resistance management remains an integral part of Bt maize crops in South Africa, in order to delay further resistance development and to prolong the viability of Bt technology.Agriculture and Animal Health
M. Sc. (Agriculture)
45
Moodley, Odeshnee. "Effectiveness of varied refugia configurations for the genetically modified maize (Zea mays L.) in Kwa-Zulu-Natal midlands." Diss., 2013. http://hdl.handle.net/10500/18319.
Full textAbstract:
Genetically modified (GM) white and yellow maize, Zea mays, has been commercially released and cultivated in South Africa since 1997/1998. The traits expressed are insect resistance and herbicide tolerance conferred by the bacteria Bacillus thuringiensis (Bt) Cry genes and Agrobacterium 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase gene, respectively. The Cry genes have been used widely to control lepidopteran insect pests but insect resistance to GM Bt crops has been a concern since the introduction of this technology. A management strategy includes refugia planting of 5% non-Bt plants, with no insecticide application, and 20%, where insecticide application is allowed. These refugia are designed to allow the survival of insect pests within restricted planted zones. However, in South Africa there are reports of Bt-resistant stem borer (Busseola fusca) (Fuller) (Lepidoptera: Noctuidae) and non-compliance with refuge planting. The aims of this study were two-fold: 1. To conduct a survey among KwaZulu-Natal (KZN) GM maize growers to ascertain information such as level of compliance with refuge planting and to determine which refugia were predominantly planted and reasons thereof; 2. To conduct a replicated field trial to determine yield, insect borer damage and economic benefit of the 5% unsprayed and 20% sprayed refuge options (including three configurations namely strip, perimeter and block and a 5 and 20% ‘refuge-in-a-bag’ option). The survey indicated that 28 out of 29 (96.6%) KZN Bt maize growers plant the 5% non-sprayed refuge with 27 (96.4%) of those respondents planting the strip configuration for the purpose of insect management (75%) and ease of planting (32.2%). The survey also showed that 7 (seven) i.e. 21.9% of KZN Bt maize growers observed borer damage and although growers are now fully compliant with refugia planting requirements, initially 7 respondents (24.1%) did not comply with or plant refugia correctly. Furthermore, 7 respondents reported insect borer damage in their maize with 4 of the 7 instances (57.1%) likely stemming from incorrectly planted refugia.
vii
No significant differences in yield or insect damage were observed between the 5 and 20% refugia for any of the planting configurations in the field trial. However due to costs involved with insecticide application and labour required for the operation in the 20% option, these treatments were less economically advantageous than the non-Bt control. The 20% block and strip configurations had a cost benefit ratio of ZAR 7.21 and ZAR 6.67 respectively, earned per R1 spent by the grower compared with ZAR 7.76 in the sprayed control. The cost-benefit comparison for the 5% block and strip configurations was ZAR 8.48 and ZAR 7.71, respectively compared with ZAR 9.44 in the unsprayed control. In addition, the 20% seed mixture limited borer damage to 4.95% when compared with 15.77% damage in the sprayed control (ANOVA, F pr = 0.124). The seed mixtures are not available commercially and the results from the survey indicated that some education and marketing by the seed companies would be advisable prior to their release to the farming community.
In order to determine which of the refuge options between 5 and 20% would be more advantageous for growers overall, regardless of the planting configuration; data were grouped and analysed. There were no significant differences in either the yield or insect damage for the 5 and 20% refugia, but the cost-benefit calculations indicated that the 5% option was more cost effective – for the 5 and 20% refugia, ZAR 7.97 and ZAR 7.15 respectively, earned per ZAR 1 spent by the grower (ANOVA, F pr. = 0.03). This is because no insecticide was used in the 5% treatments. Mean ear damage comparisons between the 5 and 20% refugia showed that the 20% refuge in the perimeter configuration incurred the least damage (2.65% ear damage) compared with 5% perimeter (10.86% ear damage), although the reasons for this are not clear.
While the results of the field trials showed no significant differences in insect damage and yield with regard to choice of refuge configuration, monitoring insect resistance management remains an integral part of Bt maize crops in South Africa, in order to delay further resistance development and to prolong the viability of Bt technology.Agriculture and Animal Health
M. Sc. (Agriculture)