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Journal articles on the topic 'Corneal and Stromal Cells'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Morishige, Naoyuki, James V. Jester, Julie Naito, et al. "Herpes simplex virus type 1 ICP0 localizes in the stromal layer of infected rabbit corneas and resides predominantly in the cytoplasm and/or perinuclear region of rabbit keratocytes." Journal of General Virology 87, no. 10 (2006): 2817–25. http://dx.doi.org/10.1099/vir.0.82076-0.

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Herpes stromal keratitis (HSK) results from the reactivation of herpes simplex virus type-1 (HSV-1) in the cornea. The subsequent corneal inflammation and neovascularization may lead to scarring and visual loss. The cellular and molecular mechanisms underlying HSK remain unknown. The presence of stromal HSV-1 viral proteins or antigens in the HSK cornea remains a subject of debate. It was recently reported that HSV-1 ICP0 rapidly diffuses out of infected rabbit corneas. To investigate further the presence of HSV-1 ICP0 in the infected cornea, particularly in the corneal stroma, ex vivo confoca

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2

Wilson, Steven E. "The Cornea: No Difference in the Wound Healing Response to Injury Related to Whether, or Not, There’s a Bowman’s Layer." Biomolecules 13, no. 5 (2023): 771. http://dx.doi.org/10.3390/biom13050771.

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Bowman’s layer is an acellular layer in the anterior stroma found in the corneas of humans, most other primates, chickens, and some other species. Many other species, however, including the rabbit, dog, wolf, cat, tiger, and lion, do not have a Bowman’s layer. Millions of humans who have had photorefractive keratectomy over the past thirty plus years have had Bowman’s layer removed by excimer laser ablation over their central cornea without apparent sequelae. A prior study showed that Bowman’s layer does not contribute significantly to mechanical stability within the cornea. Bowman’s layer doe

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3

Saban, Daniel, Taakaki Hattori, Sunil Chauhan, and Reza Dana. "Identification and characterization of langerin positive dendritic cells in the normal cornea and in transplantation. (145.27)." Journal of Immunology 184, no. 1_Supplement (2010): 145.27. http://dx.doi.org/10.4049/jimmunol.184.supp.145.27.

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Abstract A novel population of migratory dendritic cells (DCs), distinct from Langerhans cells (LC), was recently found to which also express langerin (CD207+) and reside throughout the body (skin, lung, gut, and liver). Moreover, such CD207+ DCs were shown to be important in generating immunity. Thus, we investigated whether CD207+ DCs exist in the cornea and their possible migration to lymph nodes (LN) in corneal alloimmunity. Methods: C57BL/6 corneas (n=30) were collected and epithelium separated from stroma. Tissue was pooled separately and digested enzymatically for FACS analysis of CD45,

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Liu, Cong, Xin Yu, and Yun Kou. "Effect of diagnostic ultrasound on corneal apoptosis in rats." Tropical Journal of Pharmaceutical Research 19, no. 9 (2020): 1947–51. http://dx.doi.org/10.4314/tjpr.v19i9.21.

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Purpose: To investigate the effect of diagnostic ultrasound on corneal apoptosis in rats.Methods: 24 male rats were randomly divided into 4 groups: control group, 10, 20 and 30 min group. The eyeballs of rats were irradiated continuously for different time lengths by Siemens ACUSON S2000 color Doppler ultrasound diagnostic instrument. 24 hours later, the animals were killed and the corneas were taken for Tunel apoptosis detection. The apoptosis rates of corneal epithelial cells, stromal cells and endothelial cells were calculated.Results: Apoptotic cells were detected in corneal epithelial cel

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5

Ishizaki, Masamichi, Kyoko Wakamatsu, Takakuni Matsunami, Nobuaki Yamanaka, Toshikazu Saiga, and Winston W. Y. Kao. "Appearance of myofibroblast during the healing of experimental corneal wound." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 386–87. http://dx.doi.org/10.1017/s0424820100147764.

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It is well known that corneal stroma cells (keratocytes) can transform to fibroblasts during the corneal wound healing. We have studied the expression of α-smooth muscle actin, vimentin and desmin in fibroblastic cells of the alkali-burneo and lacerated corneas in the rabbits by means of immunohistochemistry and electron microscopy.Methods. Rabbits were anesthetized, and central corneal alkali-burn and laceration were produced. The injured corneas healed for 1 day to 45 days, and 18 days embryonic rabbit corneas were immunostained with monoclonal antibodies against α-smooth muscle actin, vimen

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6

Osidak, Egor Olegovich, Andrey Yurevich Andreev, Sergey Eduardovich Avetisov, et al. "Corneal Stroma Regeneration with Collagen-Based Hydrogel as an Artificial Stroma Equivalent: A Comprehensive In Vivo Study." Polymers 14, no. 19 (2022): 4017. http://dx.doi.org/10.3390/polym14194017.

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Restoring the anatomical and functional characteristics of the cornea using various biomaterials is especially relevant in the context of a global shortage of donor tissue. Such biomaterials must be biocompatible, strong, and transparent. Here, we report a Viscoll collagen membrane with mechanical and optical properties suitable for replacing damaged stromal tissue. After removing a portion of the stroma, a Viscoll collagen membrane was implanted into the corneas of rabbits. After 6 months, the active migration of host cells into Viscoll collagen membranes was noted, with the preservation of c

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7

Bard, J. B. L., M. K. Bansal, and A. S. A. Ross. "The extracellular matrix of the developing cornea: diversity, deposition and function*." Development 103, Supplement (1988): 195–205. http://dx.doi.org/10.1242/dev.103.supplement.195.

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This paper examines the role of the extracellular matrix (ECM) in the development of the cornea. After a brief summary of the corneal structure and ECM, we describe evidence suggesting that the differentiation of neural crest (NC) cells into endothelium and fibroblasts is under the control of ocular ECM. We then examine the role of collagen I in stromal morphogenesis by comparing normal corneas with those of homozygous Movl3 mice which do not make collagen I. We report that, in spite of this absence, the cellular morphology of the Movl3 eye is indistinguishable from that of the wild type. In t

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8

Torsahakul, Chutirat, Nipan Israsena, Supaporn Khramchantuk, et al. "Bio-fabrication of stem-cell-incorporated corneal epithelial and stromal equivalents from silk fibroin and gelatin-based biomaterial for canine corneal regeneration." PLOS ONE 17, no. 2 (2022): e0263141. http://dx.doi.org/10.1371/journal.pone.0263141.

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Corneal grafts are the imperative clinical treatment for canine corneal blindness. To serve the growing demand, this study aimed to generate tissue-engineered canine cornea in part of the corneal epithelium and underlying stroma based on canine limbal epithelial stem cells (cLESCs) seeded silk fibroin/gelatin (SF/G) film and canine corneal stromal stem cells (cCSSCs) seeded SF/G scaffold, respectively. Both cell types were successfully isolated by collagenase I. SF/G corneal films and stromal scaffolds served as the prospective substrates for cLESCs and cCSSCs by promoting cell adhesion, cell

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9

Hendricks, R. L., M. Janowicz, and T. M. Tumpey. "Critical role of corneal Langerhans cells in the CD4- but not CD8-mediated immunopathology in herpes simplex virus-1-infected mouse corneas." Journal of Immunology 148, no. 8 (1992): 2522–29. http://dx.doi.org/10.4049/jimmunol.148.8.2522.

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Abstract Previous studies have revealed that the RE strain of HSV type 1 (HSV-1) induces a tissue-destructive inflammatory response in the mouse cornea that is mediated by CD4 T lymphocytes, whereas the KOS strain of HSV-1 preferentially activates CD8 T lymphocytes in the cornea. Langerhans cells (LC) normally reside only at the periphery of the cornea but can migrate centripetally after HSV-1 infection. We studied the relative contribution of LC to the corneal inflammation induced by the KOS and RE strains of HSV-1. Ten days after infection, the central one-third of RE HSV-1-infected corneas

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10

Shen, Yun-Zhi, Mi Xu, and Song Sun. "In Vivo Confocal Microscopy Observation of Cell and Nerve Density in Different Corneal Regions with Monocular Pterygium." Journal of Ophthalmology 2020 (March 23, 2020): 1–7. http://dx.doi.org/10.1155/2020/6506134.

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Purpose. To investigate the effects of pterygium on corneal cell and nerve density in patients with unilateral pterygium using in vivo laser scanning confocal microscopy (LSCM). Methods. In this cross-sectional study, 24 patients with unilateral pterygium who were treated in the Department of Ophthalmology of the Second People’s Hospital of Wuxi City from April 2018 to July 2018 were analyzed. Each eye with pterygium and its fellow eye were imaged by LSCM. The density of basal corneal epithelial cells, anterior stromal cells, posterior stromal cells, dendritic cells, and endothelial cells in p

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11

Pearlman, E., J. H. Lass, D. S. Bardenstein, et al. "Interleukin 4 and T helper type 2 cells are required for development of experimental onchocercal keratitis (river blindness)." Journal of Experimental Medicine 182, no. 4 (1995): 931–40. http://dx.doi.org/10.1084/jem.182.4.931.

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Inflammation of the corneal stroma (stromal keratitis) is a serious complication of infection with the nematode parasite Onchocerca volvulus. Because stromal keratitis is believed to be immunologically mediated in humans, we used a murine model to examine the role of T cells and T helper cell cytokines in the immunopathogenesis of these eye lesions. BALB/c mice immunized subcutaneously and injected intrastromally with soluble O. volvulus antigens (OvAg) developed pronounced corneal opacification and neovascularization. The corneal stroma was edematous and contained numerous eosinophils and mon

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12

Liu, Ming, Wenting Song, Wen Gao, et al. "Impact of Latent Virus Infection in the Cornea on Corneal Healing after Small Incision Lenticule Extraction." Microorganisms 11, no. 10 (2023): 2441. http://dx.doi.org/10.3390/microorganisms11102441.

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The aim of the present study is to analyze the impact of cornea virus latent infection on corneal healing after small incision lenticule extraction (SMILE) and predict the positive rate of virus latent infection in corneal stroma. A total of 279 patients who underwent SMILE were included in this study. Fluorescence quantitative PCR was used to detect virus infection in the lenticules, which were taken from the corneal stroma during SMILE. Herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), Epstein–Barr virus (EBV), and cytomegalovirus (CMV) were detected. Postoperative vi

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13

Bains, Kiranjit K., Robert D. Young, Elena Koudouna, Philip N. Lewis, and Andrew J. Quantock. "Cell–Cell and Cell–Matrix Interactions at the Presumptive Stem Cell Niche of the Chick Corneal Limbus." Cells 12, no. 19 (2023): 2334. http://dx.doi.org/10.3390/cells12192334.

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(1) Background: Owing to its ready availability and ease of acquisition, developing chick corneal tissue has long been used for research purposes. Here, we seek to ascertain the three-dimensional microanatomy and spatiotemporal interrelationships of the cells (epithelial and stromal), extracellular matrix, and vasculature at the corneo-scleral limbus as the site of the corneal stem cell niche of the chicken eye. (2) Methods: The limbus of developing (i.e., embryonic days (E) 16 and 18, just prior to hatch) and mature chicken eyes was imaged using scanning electron microscopy (SEM), transmissio

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14

Choi, Alexander J., Brenna S. Hefley, Hannah A. Strobel, et al. "Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells." Journal of Functional Biomaterials 16, no. 4 (2025): 118. https://doi.org/10.3390/jfb16040118.

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Corneal transplantation remains a critical treatment option for individuals with corneal disorders, but it faces challenges such as rejection, high associated medical costs, and donor scarcity. A promising alternative for corneal replacement involves fabricating artificial cornea from a patient’s own cells. Our study aimed to leverage bioprinting to develop a corneal model using human corneal stromal cells embedded in a collagen-based bioink. We generated both cellular and acellular collagen I (COL I) constructs. Cellular constructs were cultured for up to 4 weeks, and gene expression analysis

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15

Chen, Ting, Qiangxiang Li, Xiangbo Tang, Min Liao, and Hua Wang. "In Vivo Confocal Microscopy of Cornea in Patients with Terrien’s Marginal Corneal Degeneration." Journal of Ophthalmology 2019 (July 11, 2019): 1–8. http://dx.doi.org/10.1155/2019/3161843.

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This study was aimed at observing the morphological changes of the cornea with ocular in vivo confocal microscopy (IVCM) in patients with Terrien’s marginal degeneration (TMD). Ten patients (20 eyes) with TMD treated in the Department of Ophthalmology, Xiangya Hospital, and 10 healthy controls (20 eyes) were included in the current study. A detailed slit lamp microscopy, anterior segment photography, and corneal IVCM examination were performed for each eye. The density of central and marginal corneal epithelial cells, stromal cells, and subepithelial nerve fibers was compared between the two g

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GRIFFITH, M., and M. RAFAT. "Corneal Stromal Mesenchymal Stem Cells for Corneal Stroma Reconstruction." Acta Ophthalmologica 89, s248 (2011): 0. http://dx.doi.org/10.1111/j.1755-3768.2011.3374.x.

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17

Storms, G., C. Naranjo, and M. Grauwels. "Concurrent squamous cell carcinoma and hemangiosarcoma in the cornea of a cat." Vlaams Diergeneeskundig Tijdschrift 85, no. 1 (2016): 31–35. http://dx.doi.org/10.21825/vdt.v85i1.16405.

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A 14-year-old, female, spayed Domestic Shorthair cat was presented for evaluation of a dark red mass occupying about 75% of the cornea of the left eye. Furthermore, the eye presented upper eyelid trichiasis, lower eyelid entropion and a marked symblepharon. The fellow eye presented upper eyelid trichiasis, lower eyelid entropion, extensive symblepharon and chronic stromal ulcerative keratitis. Based on the results of a corneal biopsy of the left eye, a corneal squamous cell carcinoma was diagnosed and an enucleation was performed subsequently. Histopathology of the globe revealed the presence

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18

Rodríguez-Fernández, Silvia, Marcelino Álvarez-Portela, Esther Rendal-Vázquez, et al. "Analysis of Cryopreservation Protocols and Their Harmful Effects on the Endothelial Integrity of Human Corneas." International Journal of Molecular Sciences 22, no. 22 (2021): 12564. http://dx.doi.org/10.3390/ijms222212564.

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Corneal cryopreservation can partially solve the worldwide concern regarding donor cornea shortage for keratoplasties. In this study, human corneas were cryopreserved using two standard cryopreservation protocols that are employed in the Tissue Bank of the Teresa Herrera Hospital (Spain) to store corneas for tectonic keratoplasties (TK protocol) and aortic valves (AV protocol), and two vitrification protocols, VS55 and DP6. Endothelial viability and general corneal state were evaluated to determine the protocol that provides the best results. The potential corneal cryopreservation protocol was

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Krasner, K. Yu, O. V. Poveshchenko, M. A. Surovtseva, et al. "Modern anatomical and physiological bases for maintaining the transparency of the corneal stroma." Acta Biomedica Scientifica 8, no. 4 (2023): 186–98. http://dx.doi.org/10.29413/abs.2023-8.4.21.

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The article presents a literature review of the modern concept of anatomical and physiological structure and functioning of the cornea. The strict morphological structure and corneal tissue homeostasis ensure its transparency. Studying the mechanisms that regulate the constancy of the corneal tissue internal environment allows us to get closer to understanding the prospects forregenerative therapy for the corneal stroma pathology. The article discusses in detail the role and functional potential of corneal stromal cells, which are capable of reverse cytologic differentiation, which primarily e

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Wilson, Steven E. "The Yin and Yang of Mesenchymal Cells in the Corneal Stromal Fibrosis Response to Injury: The Cornea as a Model of Fibrosis in Other Organs." Biomolecules 13, no. 1 (2022): 87. http://dx.doi.org/10.3390/biom13010087.

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Mesenchymal cells (keratocytes, corneal fibroblasts, and myofibroblasts), as well as mesenchymal progenitor bone marrow-derived fibrocytes, are the major cellular contributors to stromal fibrosis after injury to the cornea. Corneal fibroblasts, in addition to being major progenitors to myofibroblasts, also have anti-fibrotic functions in (1) the production of non-basement membrane collagen type IV that binds activated transforming growth factor (TGF) beta-1 and TGF beta-2 to downregulate TGF beta effects on cells in the injured stroma, (2) the production of chemokines that modulate the entry o

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Reinstein Merjava, Stanislava, Jan Kossl, Ales Neuwirth, et al. "Presence of Protease Inhibitor 9 and Granzyme B in Healthy and Pathological Human Corneas." Biology 11, no. 5 (2022): 793. http://dx.doi.org/10.3390/biology11050793.

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The aim of this study was to find out whether protease inhibitor 9 (PI-9) and granzyme B (GrB) molecules that contribute to immune response and the immunological privilege of various tissues are expressed in healthy and pathological human corneas. Using cryosections, cell imprints of control corneoscleral discs, we showed that PI-9 was expressed particularly in the endothelium, the superficial and suprabasal epithelium of healthy corneas, limbus, and conjunctiva. GrB was localized in healthy corneal and conjunctival epithelium, while the endothelium showed weak immunostaining. The expression o

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Wu, Chun-Yen, Da-Fong Song, Zhi-Jia Chen, Chao-Sheng Hu, David Pei-Cheng Lin, and Han-Hsin Chang. "Absence of the Klotho Function Causes Cornea Degeneration with Specific Features Resembling Fuchs Endothelial Corneal Dystrophy and Bullous Keratopathy." Biology 13, no. 3 (2024): 133. http://dx.doi.org/10.3390/biology13030133.

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The Klotho loss-of-function mutation is known to cause accelerated senescence in many organs, but its effects on the cornea have not been published. The present study aims to investigate the effects of the Klotho null mutation on cornea degeneration and to characterize the pathological features. Mouse corneas of Klotho homozygous, heterozygous, and wild-type mice at 8 weeks of age for both genders were subject to pathological and immunohistological examinations. The results show an irregular topography on the corneal surface with a Klotho null mutation. Histological examinations revealed a red

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Linsenmayer, T. F., D. E. Birk, C. M. Linsenmayer, M. K. Gordon, J. K. Marchant, and J. M. Fitch. "Heterotypic fibrils and stabilizing collagens in corneal development." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 172–73. http://dx.doi.org/10.1017/s0424820100085162.

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Our studies on the embryonic development of the chick cornea have recently yielded information suggesting possible roles for different types and classes of collagens. The chick cornea develops through a series of precisely controlled temporal and spatial events involving cell differentiation, migration and extracellular matrix production and assembly. Each event involves, and is possibly dictated by, dramatic changes in the major molecular components of the extracellular matrix. Corneal morphogenesis begins with the formation of the primary corneal stroma, a dense subepithelial matrix consisti

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Zhang, Shan Shan, Jiao Jiao Li, Xiao Feng Zhang, and Shen Zhou Lu. "Corneal Matrix Repair Carrier with Composite Silk Protein Membrane." Materials Science Forum 815 (March 2015): 424–28. http://dx.doi.org/10.4028/www.scientific.net/msf.815.424.

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Corneal transplantation is the only effective way to repair the damaged corneal tissue and solve the problem of insufficient donor cornea and immune rejection. Biocompatibility and stable transparent are necessary conditions of corneal stromal cells carrier. In this paper, the acetamide/silk (AC/SF) composite membranes are studied to be applied in corneal repair material. AC/SF membranes with different blending proportions had stable transparency, good cell compatibility. X-ray diffraction was used to investigate the structure of the composite films. The acetamide inhibited the formation of la

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Jester, J. V., T. Moller-Pedersen, J. Huang, et al. "The cellular basis of corneal transparency: evidence for ‘corneal crystallins’." Journal of Cell Science 112, no. 5 (1999): 613–22. http://dx.doi.org/10.1242/jcs.112.5.613.

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In vivo corneal light scattering measurements using a novel confocal microscope demonstrated greatly increased backscatter from corneal stromal fibrocytes (keratocytes) in opaque compared to transparent corneal tissue in both humans and rabbits. Additionally, two water-soluble proteins, transketolase (TKT) and aldehyde dehydrogenase class 1 (ALDH1), isolated from rabbit keratocytes showed unexpectedly abundant expression (approximately 30% of the soluble protein) in transparent corneas and markedly reduced levels in opaque scleral fibroblasts or keratocytes from hazy, freeze injured regions of

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McKay, Tina B., Dimitrios Karamichos, Audrey E. K. Hutcheon, Xiaoqing Guo, and James D. Zieske. "Corneal Epithelial–Stromal Fibroblast Constructs to Study Cell–Cell Communication in Vitro." Bioengineering 6, no. 4 (2019): 110. http://dx.doi.org/10.3390/bioengineering6040110.

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Cell–cell communication plays a fundamental role in mediating corneal wound healing following injury or infection. Depending on the severity of the wound, regeneration of the cornea and the propensity for scar development are influenced by the acute resolution of the pro-fibrotic response mediated by closure of the wound via cellular and tissue contraction. Damage of the corneal epithelium, basement membrane, and anterior stroma following a superficial keratectomy is known to lead to significant provisional matrix deposition, including secretion of fibronectin and thrombospondin-1, as well as

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Biswas, Partha S., Kaustuv Banerjee, Bumseok Kim, Paul R. Kinchington, and Barry T. Rouse. "Role of Inflammatory Cytokine-Induced Cycloxygenase 2 in the Ocular Immunopathologic Disease Herpetic Stromal Keratitis." Journal of Virology 79, no. 16 (2005): 10589–600. http://dx.doi.org/10.1128/jvi.79.16.10589-10600.2005.

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ABSTRACT Ocular infection with herpes simplex virus (HSV) results in a blinding immunoinflammatory stromal keratitis (SK) lesion. Early preclinical events include polymorphonuclear neutrophil (PMN) infiltration and neovascularization in the corneal stroma. We demonstrate here that HSV infection of the cornea results in the upregulation of the cycloxygenase 2 (COX-2) enzyme. Early after infection, COX-2 was produced from uninfected stromal fibroblasts as an indirect effect of virus infection. Subsequently, COX-2 may also be produced from other inflammatory cells that infiltrate the cornea. The

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binte M. Yusoff, Nur Zahirah, Andri K. Riau, Gary H. F. Yam, Nuur Shahinda Humaira binte Halim, and Jodhbir S. Mehta. "Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy." Cells 11, no. 1 (2022): 178. http://dx.doi.org/10.3390/cells11010178.

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The human corneal stroma contains corneal stromal keratocytes (CSKs) that synthesize and deposit collagens and keratan sulfate proteoglycans into the stromal matrix to maintain the corneal structural integrity and transparency. In adult corneas, CSKs are quiescent and arrested in the G0 phase of the cell cycle. Following injury, some CSKs undergo apoptosis, whereas the surviving cells are activated to become stromal fibroblasts (SFs) and myofibroblasts (MyoFBs), as a natural mechanism of wound healing. The SFs and MyoFBs secrete abnormal extracellular matrix proteins, leading to corneal fibros

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Nurković, Jasmin S., Radiša Vojinović, and Zana Dolićanin. "Corneal Stem Cells as a Source of Regenerative Cell-Based Therapy." Stem Cells International 2020 (July 20, 2020): 1–11. http://dx.doi.org/10.1155/2020/8813447.

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In the past few years, intensive research has focused on corneal stem cells as an unlimited source for cell-based therapy in regenerative ophthalmology. Today, it is known that the cornea has at least two types of stem cells: limbal epithelial stem cells (LESCs) and corneal stromal stem cells (CSSCs). LESCs are used for regeneration of corneal surface, while CSSCs are used for regeneration of corneal stroma. Until now, various approaches and methods for isolation of LESCs and CSSCs and their successful transplantation have been described and tested in several preclinical studies and clinical t

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Santra, Mithun, Yu-Chi Liu, Vishal Jhanji, and Gary Hin-Fai Yam. "Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives." International Journal of Molecular Sciences 23, no. 14 (2022): 7967. http://dx.doi.org/10.3390/ijms23147967.

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A transparent cornea is paramount for vision. Corneal opacity is one of the leading causes of blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, the outcomes are challenged by a global lack of donor tissue availability. Bioengineered corneal tissues are gaining momentum as a new source for corneal wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers a new perspective on corneal regenerative medicine. Ultrathin stromal laminar tissues obtained from lenticule-based refractive correction procedure

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Kim, Kyung Wook, and Young Joo Shin. "Interactions of Corneal Endothelial Cells with Stromal Cells during Corneal Endothelial Injury." Journal of the Korean Ophthalmological Society 65, no. 2 (2024): 98–107. http://dx.doi.org/10.3341/jkos.2024.65.2.98.

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Purpose: To investigate the interaction of corneal endothelial cells (CECs) with corneal stromal cells (CSCs) on the healing of chemically induced corneal endothelial injury.Methods: Twenty-five rabbits were divided into two groups: rabbits exposed to NaOH and controls. Rabbits were clinically evaluated and sacrificed at 1 day, 1 week, 2 weeks, and 4 weeks after endothelial injury. Corneal endothelial cells and corneal stromal cells were cultured and the wound healing rate of the corneal endothelium and cytokines in the culture medium were analyzed with and without co-culture in the Transwell

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Bains, Kiranjit K., Sean Ashworth, Elena Koudouna, Robert D. Young, Clare E. Hughes, and Andrew J. Quantock. "Chondroitin Sulphate/Dermatan Sulphate Proteoglycans: Potential Regulators of Corneal Stem/Progenitor Cell Phenotype In Vitro." International Journal of Molecular Sciences 24, no. 3 (2023): 2095. http://dx.doi.org/10.3390/ijms24032095.

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Chondroitin sulphate (CS) proteoglycans with variable sulphation-motifs along their glycosaminoglycan (GAG) chains are closely associated with the stem cell niche of articular cartilage, where they are believed to influence the characteristics of the resident stem cells. Here, we investigated the immunohistochemical distribution of hybrid CS/dermatan sulphate (DS) GAGs in the periphery of the adult chicken cornea, which is the location of the cornea’s stem cell niche in a number of species, using a monoclonal antibody, 6C3, that recognises a sulphation motif-specific CS/DS GAG epitope. This re

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Rajaiya, Jaya, Amrita Saha, Xiaohong Zhou, and James Chodosh. "Human Adenovirus Species D Interactions with Corneal Stromal Cells." Viruses 13, no. 12 (2021): 2505. http://dx.doi.org/10.3390/v13122505.

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Notable among the many communicable agents known to infect the human cornea is the human adenovirus, with less than ten adenoviruses having corneal tropism out of more than 100 known types. The syndrome of epidemic keratoconjunctivitis (EKC), caused principally by human adenovirus, presents acutely with epithelial keratitis, and later with stromal keratitis that can be chronic and recurrent. In this review, we discuss the current state of knowledge regarding the molecular biology of adenovirus infection of corneal stromal cells, among which the fibroblast-like keratocyte is the most predominan

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Okudan, Süleyman, Emine Tınkır Kayıtmazbatır, Ayşe Bozkurt Oflaz, and Banu Bozkurt. "In Vivo Confocal Microscopy Findings in Corneal Stromal Dystrophies." Diagnostics 15, no. 2 (2025): 182. https://doi.org/10.3390/diagnostics15020182.

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Background/Objectives: In this study, we aim to evaluate in vivo confocal microscopy (IVCM) findings of corneal stromal dystrophies (CSDs) including granular, macular and lattice corneal dystrophy that can be used for differential diagnosis and monitoring recurrences after surgical interventions. Methods: Patients diagnosed with CSD who were followed-up in the cornea and ocular surface unit were included in this study. IVCM was performed using the Heidelberg Retina Tomograph 3, Rostock Cornea Module (Heidelberg Engineering, Germany) and anterior segment optical coherence tomography (AS-OCT) im

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Surovtseva, Maria A., Kristina Yu Krasner, Irina I. Kim, et al. "Reversed Corneal Fibroblasts Therapy Restores Transparency of Mouse Cornea after Injury." International Journal of Molecular Sciences 25, no. 13 (2024): 7053. http://dx.doi.org/10.3390/ijms25137053.

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Cell-based therapies using corneal stromal stem cells (CSSC), corneal keratocytes, or a combination of both suppress corneal scarring. The number of quiescent keratocytes in the cornea is small; it is difficult to expand them in vitro in quantities suitable for transplantation. This study examined the therapeutic effect of corneal fibroblasts reversed into keratocytes (rCF) in a mouse model of mechanical corneal injury. The therapeutic effect of rCF was studied in vivo (slit lamp, optical coherence tomography) and ex vivo (transmission electron microscopy and immunofluorescence staining). Inje

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Yeung, Vincent, Nikolay Boychev, Wissam Farhat, et al. "Extracellular Vesicles in Corneal Fibrosis/Scarring." International Journal of Molecular Sciences 23, no. 11 (2022): 5921. http://dx.doi.org/10.3390/ijms23115921.

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Communication between cells and the microenvironment is a complex, yet crucial, element in the development and progression of varied physiological and pathological processes. Accumulating evidence in different disease models highlights roles of extracellular vesicles (EVs), either in modulating cell signaling paracrine mechanism(s) or harnessing their therapeutic moiety. Of interest, the human cornea functions as a refractive and transparent barrier that protects the intraocular elements from the external environment. Corneal trauma at the ocular surface may lead to diminished corneal clarity

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Zorn-Kruppa, Michaela, Svitlana Tykhonova, Gazanfer Belge, Jürgen Bednarz, Horst A. Diehl, and Maria Engelke. "A Human Corneal Equivalent Constructed from SV40-immortalised Corneal Cell Lines." Alternatives to Laboratory Animals 33, no. 1 (2005): 37–45. http://dx.doi.org/10.1177/026119290503300107.

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Within the last decade, extensive research in the field of tissue and organ engineering has focused on the development of in vitro models of the cornea. The use of organotypic, three-dimensional corneal equivalents has several advantages over simple monolayer cultures. The aim of this study was to develop a corneal equivalent model composed of the same cell types as in the natural human tissue, but by using immortalised cell lines to ensure reproducibility and to minimise product variation. We report our success in the establishment of an SV40-immortalised human corneal keratocyte cell line (d

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Maieda Noori Ibrahim and Samira Abd Al Hussian. "Histological Changes Indused by Monosodium Glutamate on the Growth and Development of the Offsprings Albino Mice Cornea." Tikrit Journal of Pure Science 22, no. 7 (2023): 49–59. http://dx.doi.org/10.25130/tjps.v22i7.832.

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This research aim to study the effect of different doses of a Monosodium Glutamate (MSG) to cause histopathological changes in the offspring corneal for the ages 7, 15, 21, 30, and 60 days. The mothers, type Mus musculus mice has been orally administrated from 17th day of pregnancy by different concentrations (2, 4, and 8 g/Kg/ body weight) of MSG using stomach tube. Microscopic examination showed no changes in the cornea for a seventh day and administrated concentrations of 2 and 4 g / kg body weight, but observed degeneration of fibre stroma, necrosis and the reduction of the anterior epithe

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Yun, Hongmin, Alexander Rowe, Kira Lathrop, Stephen Harvey, and Robert Hendricks. "Reversible nerve damage regulates pathology in murine herpes simplex stromal keratitis. (VIR7P.1056)." Journal of Immunology 192, no. 1_Supplement (2014): 208.8. http://dx.doi.org/10.4049/jimmunol.192.supp.208.8.

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Abstract Herpes simplex type 1 (HSV-1) keratitis (HSK) is characterized by recurrent bouts of immunopathology consisting of corneal opacity, vascularization, and progressive scarring that is triggered by viral reactivation from a latent state and release from sensory nerves in the cornea. Nerve damage and loss of corneal blink reflex (BR) accompanies HSK recurrences, but testing BR is usually not part of the clinical HSK workup in humans or mouse models. Although loss of BR can lead to desiccation of the corneal surface, especially in mice that lack consensual blink reflex, a possible contribu

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Liu, Xian-Ning, Yun Chen, and Yao Wang. "Corneal stromal mesenchymal stem cells: reconstructing a bioactive cornea and repairing the corneal limbus and stromal microenvironment." International Journal of Ophthalmology 14, no. 3 (2021): 448–55. http://dx.doi.org/10.18240/ijo.2021.03.19.

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Corneal stroma-derived mesenchymal stem cells (CS-MSCs) are mainly distributed in the anterior part of the corneal stroma near the corneal limbal stem cells (LSCs). CS-MSCs are stem cells with self-renewal and multidirectional differentiation potential. A large amount of data confirmed that CS-MSCs can be induced to differentiate into functional keratocytes in vitro, which is the motive force for maintaining corneal transparency and producing a normal corneal stroma. CS-MSCs are also an important component of the limbal microenvironment. Furthermore, they are of great significance in the recon

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García, Beatriz, Olivia García-Suárez, Jesús Merayo-Lloves, et al. "Heparanase Overexpresses in Keratoconic Cornea and Tears Depending on the Pathologic Grade." Disease Markers 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/3502386.

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Background. Keratoconus has classically been defined as a noninflammatory disorder, although recent studies show elevated levels of inflammatory markers suggesting that keratoconus could be, at least in part, an inflammatory condition. Heparanase upregulation has been described in multiple inflammatory disorders. In this article, we study the differential expression of heparanase in cornea and tears from keratoconus patients and healthy controls. Methods. A transcriptomic approach was used employing quantitative polymerase chain reaction to analyze the expression of heparanase and heparanase 2

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Meshko, Berbang, Thomas L. A. Volatier, Johanna Mann, et al. "Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population." International Journal of Molecular Sciences 25, no. 17 (2024): 9702. http://dx.doi.org/10.3390/ijms25179702.

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Corneal transparency and avascularity are essential for vision. The avascular cornea transitions into the vascularized conjunctiva at the limbus. Here, we explore a limbal stromal cell sub-population that expresses ABCB5 and has mesenchymal stem cell characteristics. Human primary corneal stromal cells were enriched for ABCB5 by using FACS sorting. ABCB5+ cells expressed the MSC markers CD90, CD73, and CD105. ABCB5+ but not ABCB5− cells from the same donor displayed evidence of pluripotency with a significantly higher colony-forming efficiency and the ability of trilineage differentiation (ost

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Wu, Jian, Yiqin Du, Mary M. Mann, James L. Funderburgh, and William R. Wagner. "Corneal stromal stem cells versus corneal fibroblasts in generating structurally appropriate corneal stromal tissue." Experimental Eye Research 120 (March 2014): 71–81. http://dx.doi.org/10.1016/j.exer.2014.01.005.

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Pardue, M. T., J. G. Sivak, and K. M. Kovacs. "Corneal anatomy of marine mammals." Canadian Journal of Zoology 71, no. 11 (1993): 2282–90. http://dx.doi.org/10.1139/z93-320.

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The corneal anatomy of fin whales (Balaenoptera physalus), minke whales (Balaenoptera acutorostrata), harp seals (Phoca groenlandica), ringed seals (Phoca hispida), and bearded seals (Eriganthus barbatus) was examined to determine if marine mammals have evolved specialized corneas for life in a marine habitat. One to seven eyes of each species were analyzed: paraffin sections stained with haematoxylin and eosin for light microscopy; and ultrathin sections for transmission electron microscopy. All corneas contain the five typical mammalian layers: epithelium, Bowman's layer, stroma, Descemet's

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Tumpey, Terrence M., Hao Cheng, Donald N. Cook, Oliver Smithies, John E. Oakes та Robert N. Lausch. "Absence of Macrophage Inflammatory Protein-1α Prevents the Development of Blinding Herpes Stromal Keratitis". Journal of Virology 72, № 5 (1998): 3705–10. http://dx.doi.org/10.1128/jvi.72.5.3705-3710.1998.

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ABSTRACT Prior studies in our laboratory have suggested that the CC chemokine macrophage inflammatory protein-1α (MIP-1α) may be an important mediator in the blinding ocular inflammation which develops following herpes simplex virus type 1 (HSV-1) infection of the murine cornea. To directly test this hypothesis, MIP-1α-deficient (−/−) mice and their wild-type (+/+) counterparts were infected topically on the scarified cornea with 2.5 × 105 PFU of HSV-1 strain RE and subsequently graded for corneal opacity. Four weeks postinfection (p.i.), the mean corneal opacity score of −/− mice was 1.1 ± 0.

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Wang, Xiaokun, Liam Chung, Joshua Hooks, et al. "Type 2 immunity induced by bladder extracellular matrix enhances corneal wound healing." Science Advances 7, no. 16 (2021): eabe2635. http://dx.doi.org/10.1126/sciadv.abe2635.

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The avascular nature of cornea tissue limits its regenerative potential, which may lead to incomplete healing and formation of scars when damaged. Here, we applied micro- and ultrafine porcine urinary bladder matrix (UBM) particulate to promote type 2 immune responses in cornea wounds. Results demonstrated that UBM particulate substantially reduced corneal haze formation as compared to the saline-treated group. Flow cytometry and gene expression analysis showed that UBM particulate suppressed the differentiation of corneal stromal cells into α-smooth muscle actin–positive (αSMA+) myofibroblast

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Abou-Slaybi, Abdo, Arsia Jamali, Deshea L. Harris, Yashar Seyed-Razavi, and Pedram Hamrah. "Analysis of leukocyte populations and nerves in developing murine corneas." Journal of Immunology 202, no. 1_Supplement (2019): 117.15. http://dx.doi.org/10.4049/jimmunol.202.supp.117.15.

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Abstract The avascular cornea is the most densely innervated tissue in the body and is endowed with resident bone marrow-derived cells. We have identified a novel subset of resident corneal plasmacytoid dendritic cells (pDCs). We have shown pDCs support corneal nerves through release of nerve growth factor (NGF). This study was to examine the presence of pDCs in the developing cornea. We examined corneas from E17, P2, and 8 week adult C57BL/6 mice. Corneas were stained for the pan-leukocyte marker CD45, the pDC marker plasmacytoid dendritic cell antigen-1 (PDCA-1), the pan-neuronal marker bIII

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An, Seungwon, Khandaker Anwar, Mohammadjavad Ashraf, Kyu-Yeon Han, and Ali R. Djalilian. "Chlorine-Induced Toxicity on Murine Cornea: Exploring the Potential Therapeutic Role of Antioxidants." Cells 13, no. 5 (2024): 458. http://dx.doi.org/10.3390/cells13050458.

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Chlorine (Cl2) exposure poses a significant risk to ocular health, with the cornea being particularly susceptible to its corrosive effects. Antioxidants, known for their ability to neutralize reactive oxygen species (ROS) and alleviate oxidative stress, were explored as potential therapeutic agents to counteract chlorine-induced damage. In vitro experiments using human corneal epithelial cells showed decreased cell viability by chlorine-induced ROS production, which was reversed by antioxidant incubation. The mitochondrial membrane potential decreased due to both low and high doses of Cl2 expo

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Gillette-Ferguson, Illona, Katrin Daehnel, Amy G. Hise, et al. "Toll-Like Receptor 2 Regulates CXC Chemokine Production and Neutrophil Recruitment to the Cornea in Onchocerca volvulus/ Wolbachia-Induced Keratitis." Infection and Immunity 75, no. 12 (2007): 5908–15. http://dx.doi.org/10.1128/iai.00991-07.

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ABSTRACT The filarial nematode Onchocerca volvulus is the causative organism of river blindness. Our previous studies demonstrated an essential role for endosymbiotic Wolbachia bacteria in corneal disease, which is characterized by neutrophil infiltration into the corneal stroma and the development of corneal haze. To determine the role of Toll-like receptors (TLRs) in neutrophil recruitment and activation, we injected a soluble extract of O. volvulus containing Wolbachia bacteria into the corneal stromata of C57BL/6, TLR2−/−, TLR4−/−, TLR2/4−/−, and TLR9−/− mice. We found an essential role fo

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Ghazal, Wassim, Cristina Georgeon, Kate Grieve, Nacim Bouheraoua, and Vincent Borderie. "Multimodal Imaging Features of Schnyder Corneal Dystrophy." Journal of Ophthalmology 2020 (March 23, 2020): 1–10. http://dx.doi.org/10.1155/2020/6701816.

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Objective. To describe the multimodal imaging of Schnyder corneal dystrophy. Methods. Seven eyes of seven patients (5 female and 2 male patients) aged 52 to 92 years were included in this prospective observational study. Diagnosis of SCD was confirmed by histology after keratoplasty. In vivo multimodal imaging consisted of spectral domain-optical coherence tomography with cross sections, en face scans, corneal pachymetry, and epithelial mapping, and in vivo confocal microscopy was recorded. Ex vivo full-field optical coherence tomography scans of two corneal buttons were analyzed. The seven co

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