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Journal articles on the topic 'Cornete Medio'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Saffon-Botero, Gabriel Eduardo. "Concha bullosa gigante: reporte de un caso." ACTA DE OTORRINOLARINGOLOGÍA & CIRUGÍA DE CABEZA Y CUELLO 47, no. 4 (2020): 238–40. http://dx.doi.org/10.37076/acorl.v47i4.427.

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La concha bullosa es una variante anatómica del cornete medio nasal. Se reporta un caso de una mujer de 21 años con síntomas de obstrucción nasal severa de ambas fosas nasales. Al examen físico se aprecia gran masa que ocupa la fosa nasal izquierda y una severa desviación septal a la derecha. La tomografía computarizada muestra una concha bullosa gigante izquierda, con neumatización del cornete superior izquierdo y una desviación y adelgazamiento septal severos hacia la fosa nasal derecha. Se realiza cirugía endoscópica con resección de la concha bullosa y corrección de la neumatización del co

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Hernández-Alatriste, Genaro Efraín, Fernando Torres-Zapiain, and Martha Alicia Hernández-González. "Efectividad del colgajo de cornete medio para tratamiento de fístulas espontáneas." Revista Médica del Instituto Mexicano del Seguro Social 63, no. 3 (2025): e6574. https://doi.org/10.5281/zenodo.15178461.

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<strong>Resumen</strong> <strong>Introducción: </strong>actualmente no hay certeza sobre cuál es el mejor tratamiento para las fístulas espontáneas de líquido cefalorraquídeo (LCR). Este estudio buscó probar la efectividad del colgajo pediculado de cornete medio (CPCM) en el tratamiento de dichas fístulas, localizadas en el piso anterior de la base de cráneo (PABC). <strong>Objetivo:</strong> demostrar la disminución en la recidiva de las fístulas espontáneas de LCR en el PABC tratadas mediante CPCM v

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Alba García, J. R., M. Armengot Carceller, C. A. Pérez Fernández, et al. "Una forma excepcional de displasia fibrosa craneofacial: Displasia fibrosa de cornete medio." Acta Otorrinolaringológica Española 53, no. 4 (2002): 291–94. http://dx.doi.org/10.1016/s0001-6519(02)78312-x.

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Marino Silva, José Antonio, Gerardo Gutiérrez Santos, Jaime Fernández Espinosa, Teresa Cristina Cuesta Mejías, and Manuel Guerrero Hernández. "Hemangioma capilar lobular nasal del cornete medio: una causa rara de epistaxis recurrente." Anales Médicos de la Asociación Médica del Centro Médico ABC 64, no. 4 (2019): 313–17. http://dx.doi.org/10.35366/bc194n.

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Tobar Bonilla, Luz Nelly, Camilo Andrés Reyes Gelves, and Omar Alberto Gutiérrez Guauque. "Biometría etmoidal, del complejo osteomeatal y sus variantes anatómicas. Estudio en cadáveres." ACTA DE OTORRINOLARINGOLOGÍA & CIRUGÍA DE CABEZA Y CUELLO 39, no. 3 (2018): 119–27. http://dx.doi.org/10.37076/acorl.v39i3.242.

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La anatomía del etmoides y del complejo osteomeatal ha sido la base fundamental de muchos avances científicos especialmente en el campo de la cirugía endoscópica. Por ello el objetivo del presente trabajo es describir la anatomía etmoidal, del complejo osteomeatal y sus variantes anatómicas en los cadáveres del anfiteatro de la Universidad del Rosario, un estudio observacional descriptivo tipo serie de casos donde se tomaron 20 cabezas, se hicieron disecciones y mediciones en ellas. Se evaluó la concordancia interobservador e intraobservador en las variables numéricas con el paquete SPSS versi

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Fernández R, Lara, Paul Boettiger B, Karol Baksai E, and Paul H. Délano R. "Cefalea rinogénica ¿mito o realidad?: Displasia fibrosa de cornete medio como causa de algia facial." Revista de otorrinolaringología y cirugía de cabeza y cuello 72, no. 3 (2012): 278–81. http://dx.doi.org/10.4067/s0718-48162012000300011.

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7

Villanueva, Yave Elsa Catalina, Montoro Lourdes Verónica Romaní, Zeballos Norka Mariella Vásquez, and Zare Deliz Melina Vásquez. "Comparación de Técnicas Quirúrgicas para Medialización del Cornete Medio en Cirugía Endoscópica Nasosinusal en Sinusitis Crónica con o sin Pólipos." RevCen 2 (April 11, 2025): 10–14. https://doi.org/10.5281/zenodo.15196368.

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8

Garzón, Héctor, Victor Oróstegui, Margarita Maldonado, and Jorge Luís Herrera. "Sinusitis crónica y prevalencia de la concha bullosa." Revista Repertorio de Medicina y Cirugía 14, no. 1 (2005): 28–30. http://dx.doi.org/10.31260/repertmedcir.v14.n1.2005.374.

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Introducción: la neumatización del etmoides es un evento de alta complejidad y variabilidad, que se refleja en la existencia de estructuras etmoidales tales como la concha bullosa (CB), cornete medio paradójico, celdillas de Haller y bulla etmoidal prominente entre otras. Existen estadísticas foráneas referentes a la incidencia y prevalencia de estas variantes de neumatización, pero en nuestro medio carecemos de datos al respecto. El presente trabajo tiene como objetivo general establecer la prevalencia de CB en tomografias computarizadas (TAC) de cara y de manera anexa observar si existe rela

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Cuadra López, Manuel Ernesto, Roma Cristina Charaja Vildoso, Esmeralda Solier de la Cruz, et al. "Prevalencia de variantes anatómicas nasosinusales evaluadas por tomografía computada en pacientes de la clínica internacional enero-diciembre 2019." Interciencia médica 11, no. 1 (2021): 6–12. http://dx.doi.org/10.56838/icmed.v11i1.30.

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Introducción: La tomografía computada (TC) ha ganado terreno como método de diagnóstico de patologías de la nariz y los senos paranasales, así como en el estudio prequirúrgico de los pacientes ya que brinda gran detalle de las estructuras óseas y variantes anatómicas de esta región. Materiales y métodos: Estudio observacional descriptivo transversal retrospectivo de los pacientes del Servicio de Otorrinolaringología de la Red de Clínica Internacional que fueron sometidos a TC entre enero-diciembre 2019, fueron evaluadas 11 categorías de variantes anatómicas: desviación septal, cornete medio, m

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Namitha, Bhuvaneshwari, Munusamy Rajendran Chitra, Mathevan Bhavya, et al. "A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells." Biotechnology Letters 43, no. 6 (2021): 1241–51. http://dx.doi.org/10.1007/s10529-021-03116-y.

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Abstract Purpose McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. Methods Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4 °C for 96 h. Slit lamp examination

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Petithomme, Rubens, Ranit Karmakar, John Lohmeier, Staci Terrin, Ellen H. Koo, and Allen O. Eghrari. "Comparison of Corneal Endothelial Cell Density and Morphology With Optisol-GS and Life4C Storage Media in the Eye Bank: A 5-Year Retrospective Analysis." Eye Banking and Corneal Transplantation 2, no. 4 (2023): e0019. http://dx.doi.org/10.1097/ebct.0000000000000019.

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Purpose: Optisol-GS and Life4C are corneal storage media used by eye banks worldwide. We sought to determine whether either solution was associated with superior corneal endothelial cell density (ECD) or morphology in a large cohort of donor corneas. Methods: From January 2016 to December 2020, 10,316 corneas from 5624 unique donors were acquired and analyzed at Rocky Mountain Lions Eye Bank. In April 2019, Life4C replaced Optisol-GS as the sole storage medium. We compared ECD and morphology before and after April 2019 and excluded corneas processed within the transition period. Univariable an

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Aktan, Murad, Berrin Okka, Mehmet Okka, and Selcuk Duman. "Improvement In Rabbit Corneal Cell Suspension Viability After Freezing With Gingko Biloba Extrakt." Acta Medica (Hradec Kralove, Czech Republic) 50, no. 2 (2007): 145–48. http://dx.doi.org/10.14712/18059694.2017.72.

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We investigated whether the addition of Gingko Biloba extract (EGb 761) to rabbit corneal epithelial medium before cell freezing improved cell viability after freezing then thawing. After removal of corneas, they were treated with enzymes and the corneal epithelium was prepared as a single cell suspension in freezing media with or without EGb 761. After freezing for two weeks then thawing, a higher cell viability was found in the cornea cell suspensions which had been frozen pretreated with EGb 761 in the media. The improvement with corneal cell viability with EGb 761 pretreatment is postulate

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Ong, Hon Shing, Gary Peh, Dawn Jin Hui Neo, et al. "A Novel Approach of Harvesting Viable Single Cells from Donor Corneal Endothelium for Cell-Injection Therapy." Cells 9, no. 6 (2020): 1428. http://dx.doi.org/10.3390/cells9061428.

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Donor corneas with low endothelial cell densities (ECD) are deemed unsuitable for corneal endothelial transplantation. This study evaluated a two-step incubation and dissociation harvesting approach to isolate single corneal endothelial cells (CECs) from donor corneas for corneal endothelial cell-injection (CE-CI) therapy. To isolate CECs directly from donor corneas, optimization studies were performed where donor Descemet’s membrane/corneal endothelium (DM/CE) were peeled and incubated in either M4-F99 or M5-Endo media before enzymatic digestion. Morphometric analyses were performed on the is

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Jeng, Bennie H., David M. Meisler, Joe G. Hollyfield, Jason T. Connor, Kulwant S. Aulak, and Dennis J. Stuehr. "Nitric Oxide Generated by Corneas in Corneal Storage Media." Cornea 21, no. 4 (2002): 410–14. http://dx.doi.org/10.1097/00003226-200205000-00015.

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15

Winkler, B. S., M. V. Riley, M. I. Peters, and F. J. Williams. "Chloride is required for fluid transport by the rabbit corneal endothelium." American Journal of Physiology-Cell Physiology 262, no. 5 (1992): C1167—C1174. http://dx.doi.org/10.1152/ajpcell.1992.262.5.c1167.

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The role of chloride in fluid transport of the rabbit corneal endothelium was examined by measuring changes in corneal thickness following ion substitutions or addition of ion transport inhibitors in media superfusing the isolated tissue. Normal fluid transport is indicated by maintenance of constant thickness in a fresh cornea or thinning (deturgescence) of a preswollen deepithelialized cornea to its initial thickness at approximately 40 microns/h. These patterns are seen when tissues are superfused with HCO(3-)-Ringer containing 114 mM Cl-. When Cl- was substituted with gluconate, glucuronat

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Torres G, Arnulfo, and Helen Silva L. "Maxilectomia parcial por Degloving en cáncer - nasosinusal Partial maxillectomy by Degloving in sinonasal cancer." ACTA DE OTORRINOLARINGOLOGÍA & CIRUGÍA DE CABEZA Y CUELLO 42, no. 4 (2018): 243–47. http://dx.doi.org/10.37076/acorl.v42i4.148.

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Introducción: Los tumores de Senos paranasales y cavidad nasal representan el 0.2% de todos los cánceres; son más frecuentes en hombres y predominantemente unilateral; los tipos histológicos más comunes son el Carcinoma escamocelular y los carcinomas de glándulas salivales menores, dentro del cual, se encuentra el carcinoma adenoideo quístico. Las bases del tratamiento para las lesiones resecables combinan radioterapia y cirugía radical cuyo abordaje puede ser endoscópico, abierto (ej. degloving mediofacial) o la combinación de ambos. Objetivo: Describir un abordaje por degloving para la reali

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Gupta, Avinash, Ramyash Singh Yadav, Chiranji Rai, and Anzar Ahmed Ansari. "Effectiveness of intrastromal voriconazole injection in the management of deep non healing fungal corneal ulcer." Indian Journal of Clinical and Experimental Ophthalmology 8, no. 3 (2022): 345–50. http://dx.doi.org/10.18231/j.ijceo.2022.071.

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One of the most difficult conditions to cure is often corneal fungus infections. Due to low stromal penetration, current topical antifungal medications are not very successful in the treatment of fungal keratitis, which makes it challenging to treat cases of deep fungal corneal ulcers. To assess the efficiency of voriconazole intra stromal injection in the treatment of deep fungal corneal ulcers that donot heal. In this prospective interventional study of thirty patients, deep non-healing fungal corneal ulcers were successfully treated by combining intrastromal voriconazole with topical therap

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Javagal, Akshatha, Sandeep K, Kavitha Chikkanayakanahalli, Pavana Acharya, Sreelekshmi S R, and Narendra N. "Assessment on the evaluation of corneal endothelial cell morphology and cell count in cataract with pseudoexfoliation." Indian Journal of Clinical and Experimental Ophthalmology 9, no. 1 (2023): 92–96. http://dx.doi.org/10.18231/j.ijceo.2023.018.

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To assess the cell morphology & evaluate corneal endothelial cell count in patients suffering from cataract with pseudoexfoliation (PEX).A cross-sectional study was planned and conducted in 40 eyes of patients, corneal endothelial cell density (CD), central corneal thickness (CCT) and coefficient of variation (CV) & percentage of hexagonal cells (6A) in cell size were assessed with application of specular microscopeThe mean age of patients with cataract & PEX was 68.5 years. 58% were males. Lower CD was found in 40% patients of 50-59 years & 60% patients 60-69 years. Patients a

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Reddy, Chinnangolla Viveknandini, and M. H. Reddy. "A comparative study of central corneal thickness in diabetics and non-diabetics using ultrasonic pachymetry." Indian Journal of Clinical and Experimental Ophthalmology 7, no. 3 (2021): 554–61. http://dx.doi.org/10.18231/j.ijceo.2021.109.

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To determine association between central corneal thickness and type 2 diabetes mellitus in patients attending outpatient department of Ophthalmology at a tertiary care centre in North Karnataka.This is a cross-sectional study conducted over a period of April 2018 – September 2020 on patients attending outpatient department of Ophthalmology at a tertiary care centre in North Karnataka. Study includes 168 subjects divided into 3 groups: 40 diabetics whose duration &#62;10 years46 diabetics whose duration ≤10 yearsand 82 controls.Detailed ophthalmic examination was conducted in all patients a

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Müller, Lisa, Philipp Niklas Ostermann, Heiner Schaal, et al. "RT-PCR Testing of Organ Culture Medium for Corneal Storage Fails to Detect SARS-CoV-2 Infection Due to Lack of Viral Replication." Pathogens 11, no. 2 (2022): 133. http://dx.doi.org/10.3390/pathogens11020133.

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Concerns of possible transmission of SARS-CoV-2 from donors to patients by corneal transplantation have caused a decline in corneal transplantations. Graft culture media are routinely tested for infectious risks, but it is unclear whether this constitutes a viable means to avoid transmitting SARS-CoV-2 via keratoplasty. We found that SARS-CoV-2 RNA was not present in the medium after seven days of organ culture of corneas from donors (n = 4), who were SARS-CoV-2-positive upon tissue procurement. These medium samples showed no presence of viral RNA. To pursue this question under controlled cond

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Gimenes, Izabela, Andréa V. Braga Pintor, Mariana da Silva Sardinha, et al. "Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review." Applied Sciences 12, no. 14 (2022): 7079. http://dx.doi.org/10.3390/app12147079.

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Optisol-GS is the most widely used pharmaceutical composition to preserve corneas for transplantation. This systematic review investigated the effects of different cold corneal storage media (CCSM) compared with Optisol-GS on the quality of stored corneas. The literature was searched throughout May 2022 on six databases and grey literature. Studies including corneas (population) exposed to distinct cold storage media (exposure) and Optisol-GS (comparison) that reported qualitative and/or quantitative parameters of cornea quality (outcome) were included. Methodological quality was assessed usin

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Gangur-Powell, Tabitha, Stefano Ferrari, Diego Ponzin, and Alessandro Ruzza. "Pre-Descemet Endothelial Keratoplasty: A Novel Technique to Optimize Type-1 Bubble Formation." Cornea Open 4, no. 3 (2025): e0062. https://doi.org/10.1097/coa.0000000000000062.

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Purpose: The purpose of this study was to propose a novel method for pre-Descemet endothelial keratoplasty (PDEK) using a liquid bubble in place of the typically used sterilized air. Methods: 71 human donor corneas were stored in transport medium (Minimum Essential Medium, HEPES, sodium pyruvate, l-glutamine, serum, antibiotics, and 6% Dextran T-500, used at 31°C) between 1 and 66 days. All samples underwent PDEK graft preparation. 24 of the samples were injected with air, and 47 were injected with liquid. Liquid injection was then performed on 21 corneas in storage medium (as transport medium

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Singh, Manisha, Preeti Waskel, Sooraj Kubrey, Aditi Dubey, and Kavita Kumar. "Evaluating the effect of donor and recipient factors on graft survival after penetrating keratoplasty." Indian Journal of Clinical and Experimental Ophthalmology 8, no. 3 (2022): 378–82. http://dx.doi.org/10.18231/j.ijceo.2022.077.

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Corneal diseases are one of the leading causes of visual loss and blindness, after cataracts and glaucoma across the globe. Management of corneal blindness requires corneal transplantation which is dependent on voluntary corneal donations and needs tremendous post-operative care and follow-up. The success of the procedure depends upon various donor and recipient factors that need to be studied.A prospective study was conducted in the eye bank of the tertiary care eye center in central India after getting the approval of the institutional ethical committee. A total of 26 eyes of 13 donors were

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Makra, Zita, Tamás Tuboly, and Gábor Bodó. "Penetrating keratoplasty and graft rejection in eight horses." Acta Veterinaria Hungarica 61, no. 2 (2013): 160–74. http://dx.doi.org/10.1556/avet.2013.002.

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The aim of this study was to describe long-term follow-up and difference in immune reactions in the tear film following penetrating keratoplasty (PK) in horses when differently preserved corneas were utilised. This report describes for the first time the use of corneal grafts preserved in tissue culture media in equine PK. Eight experimental horses with normal eyes were included and freshly harvested, frozen or preserved corneal grafts were used for the PK. The graft-taking technique and storage, PK surgery, postoperative treatments and complications are described. The mean postoperative follo

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Krichevskaya, G. I., L. A. Kovaleva, I. D. Zyurnyayeva, P. V. Makarov, and A. E. Andryushin. "The Effectiveness of PCR in Diagnosis of Fungal Keratitis." Ophthalmology in Russia 17, no. 4 (2020): 824–29. http://dx.doi.org/10.18008/1816-5095-2020-4-824-829.

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Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose. To evaluate the effectiveness of polymerase chain reaction (PCR) for the detection of fungal etiology in comparison with the conventional diagnostic methods in cases with suspected fungal corneal ulcer.Patients and methods. Seven patients with severe corneal ulcers with more than 3 weeks duration. Corneal scarpings and corneal buttons from seven patients who had undergone t

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Colabelli Gisoldi, Rossella Anna Maria, Giulio Pocobelli, Umberto Rodella, et al. "Retrospective Clinical Outcomes of Keratoplasty Using Human Donor Corneas Preserved in Eusol-C Hypothermic Storage Medium." Journal of Clinical Medicine 13, no. 24 (2024): 7606. https://doi.org/10.3390/jcm13247606.

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Objective: To evaluate the clinical outcomes of cornea transplantation (penetrating keratoplasty, Descemet membrane endothelial keratoplasty, Descemet stripping automated endothelial keratoplasty, and deep anterior lamellar keratoplasty) using donor corneas stored in Eusol-C hypothermic storage medium compared to corneas stored in organ-culture. Methods: The clinical outcomes of 92 patients who underwent corneal transplantation with human donor corneas stored in Eusol-C medium at 2–8 °C were retrospectively evaluated. The control group consisted of 169 patients who received corneas organ-cultu

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Maurin, Corantin, Zhiguo He, Marielle Mentek, et al. "Exploration of the ocular surface infection by SARS-CoV-2 and implications for corneal donation: An ex vivo study." PLOS Medicine 19, no. 3 (2022): e1003922. http://dx.doi.org/10.1371/journal.pmed.1003922.

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Background The risk of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) transmission through corneal graft is an ongoing debate and leads to strict restrictions in corneas procurement, leading to a major decrease in eye banking activity. The aims of this study are to specifically assess the capacity of human cornea to be infected by SARS-CoV-2 and promote its replication ex vivo, and to evaluate the real-life risk of corneal contamination by detecting SARS-CoV-2 RNA in corneas retrieved in donors diagnosed with Coronavirus Disease 2019 (COVID-19) and nonaffected donors. Methods and

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Seeber, Judith W., Michaela Zorn-Kruppa, Simone Lombardi-Borgia, et al. "Characterisation of Human Corneal Epithelial Cell Cultures Maintained under Serum-free Conditions." Alternatives to Laboratory Animals 36, no. 5 (2008): 569–83. http://dx.doi.org/10.1177/026119290803600512.

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Three-dimensional tissue constructs have been proposed as in vitro screening models for ocular irritancy. Based on our previous studies, in which a full-thickness corneal model based exclusively on SV40-immortalised cell lines was generated, we have currently evaluated the effects of a range of commercially-available cell culture media on several cellular parameters in cultures of a human corneal epithelial (HCE) cell line. This cell line was used in an attempt to establish a rational basis for the development of serum-free culture media for the assembly and long-term tissue culture of full-th

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Parkin, B. T., V. A. Smith, and D. L. Easty. "The Control of Matrix Metalloproteinase-2 Expression in Normal and Keratoconic Corneal Keratocyte Cultures." European Journal of Ophthalmology 10, no. 4 (2000): 276–85. http://dx.doi.org/10.1177/112067210001000402.

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Purpose Early phase keratoconic comeas and their cultured keratocytes abnormally produce the Mr 62,000 form of the matrix metalloproteinase-2 (MMP-2). It is known that platelet derived growth factor (PDGF) and transforming growth factor-β (TGF-β) are involved in the regulation of MMP activity and tissue inhibitor of metalloproteinase (TIMP) production in non-ocular tissues. The purpose of this enquiry was to determine whether these growth factors also play a role in the activity and/or production of corneal MMP-2 and TIMP, and whether their activity could account for the existence of the Mr 62

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Barhate, Amruta, Prasad Pore, and Amol Nath. "Knowledge and attitude about eye donation amongst anganwadi workers in a rural block of Pune district in India: a quasi experimental study." International Journal Of Community Medicine And Public Health 7, no. 9 (2020): 3669. http://dx.doi.org/10.18203/2394-6040.ijcmph20203535.

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Background: Corneal diseases constitute a significant cause of visual impairment and blindness in India. Corneal transplantation is a major solution for this. In order to increase the corneal transplants, motivation of rural population is must to increase eye donation rates. Anganwadi workers (AWWs) constitute a major potential source of information in rural areas which needs to be educated to increase the procurement of corneas. The aims and objectives of the study was to compare the knowledge and attitude about eye donation amongst AWWs in rural Pune before and after intervention.Methods: Th

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Alling, Christopher R., Chin-Chi Liu, Ingeborg M. Langohr, et al. "Assessment of Cidofovir for Treatment of Ocular Bovine Herpesvirus-1 Infection in Cattle Using an Ex-Vivo Model." Viruses 13, no. 10 (2021): 2102. http://dx.doi.org/10.3390/v13102102.

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Bovine herpesvirus-1 (BoHV-1) infection contributes to keratoconjunctivitis, respiratory disease, and reproductive losses in cattle. The objective of this study was to determine the most appropriate ophthalmic antiviral agent for BoHV-1 inhibition using in-vitro culture and novel ex-vivo bovine corneal modeling. Half-maximal inhibitory concentrations of BoHV-1 were determined for cidofovir, ganciclovir, idoxuridine, and trifluridine via in-vitro plaque reduction assays. In-vitro cytotoxicity was compared amongst these compounds via luciferase assays. Trifluridine and cidofovir were the most po

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Ramos, Tiago, Mohit Parekh, Stephen B. Kaye, and Sajjad Ahmad. "Epithelial Cell-Derived Extracellular Vesicles Trigger the Differentiation of Two Epithelial Cell Lines." International Journal of Molecular Sciences 23, no. 3 (2022): 1718. http://dx.doi.org/10.3390/ijms23031718.

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Extracellular vesicles (EVs), specifically exosomes, carry a cell-type dependent cargo that is transported to the recipient cell and translated in the presence of a required machinery. Differences in the cargo carried by the corneal and conjunctival-derived EVs could be the agent that triggers the transdifferentiation of these two cell populations. Therefore, this study investigates the role of EVs in triggering the plasticity of corneal and conjunctival epithelial cells and identifies prospective miRNA and genes responsible for maintaining ocular surface homeostasis. The EVs were extracted fr

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Wille, Diana, Joana Heinzelmann, Astrid Kehlen, et al. "New Safety Aspects in Corneal Donation—Studies on SARS-CoV-2-Positive Corneal Donors." Journal of Clinical Medicine 11, no. 12 (2022): 3312. http://dx.doi.org/10.3390/jcm11123312.

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In the tissue donation field, to prevent pathogen transmission, all donors are screened by postmortem swabs for SARS-CoV-2 using qRT–PCR. Corneas from donors who tested positive for SARS-CoV-2 were subjected to further investigations. Corneal transplants and culture medium from positive donors were cultured under appropriate safety conditions for further analyses. Cornea tissue samples, including sclera/limbus/cornea, and culture media were taken at different time points for testing for SARS-CoV-2 using qRT–PCR, immunohistochemistry (IHC) and subgenomic RNA (sgRNA) analysis. Between January an

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Riley, Michael V., Barry S. Winkler, Catherine A. Starnes, and Margaret I. Peters. "Fluid and ion transport in corneal endothelium: insensitivity to modulators of Na+-K+-2Cl−cotransport." American Journal of Physiology-Cell Physiology 273, no. 5 (1997): C1480—C1486. http://dx.doi.org/10.1152/ajpcell.1997.273.5.c1480.

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The role of Na+-K+-2Cl−cotransport in ion and fluid transport of the corneal endothelium was examined by measuring changes in corneal hydration and uptake of86Rb by the endothelial cell layer. Isolated, intact rabbit corneas maintain normal hydration when they are superfused at the endothelial surface with bicarbonate ([Formula: see text])-Ringer solutions as a result of equilibrium between active ion and fluid transport out of the stromal tissue and leak of fluid into stromal tissue from the aqueous humor. Furosemide and bumetanide did not alter this equilibrium when they were added to the su

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Sánchez Rivera, Camila Andrea, and Myriam Teresa Mayorga. "Variación de la asfericidad corneal en pacientes miopes sometidos a cirugía refractiva LASIK (Laser-Assisted in Situ Keratomileusis) o LASEK (Laser-Assisted Subepithelial Keratomileusis)." Ciencia & Tecnología para la Salud Visual y Ocular 14, no. 2 (2016): 71. http://dx.doi.org/10.19052/sv.3877.

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<p align="justify"><em>Objetivo</em>: analizar, por medio de una revisión sistemática de la literatura, la variación de la asfericidad corneal en pacientes miopes corregidos con cirugía LASIK (Laser-Assisted in Situ Keratomileusis) o LASEK (Laser-Assisted Subepithelial Keratomileusis). <em>Métodología</em>: se realizó una búsqueda de estudios primarios en las bases de datos PubMed, Scopus y Science Direct, a través del <em>software</em> Vantage Point; se recolectaron 229 publicaciones, de las cuales se seleccionaron diecinueve que cumplían con los crit

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Siebeck, Ulrike E., and N. Justin Marshall. "Transmission of ocular media in labrid fishes." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 355, no. 1401 (2000): 1257–61. http://dx.doi.org/10.1098/rstb.2000.0679.

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Wrasses (Labridae) are the second largest family of fishes on the Great Barrier Reef (after the Gobiidae) and, in terms of morphology and lifestyle, one of the most diverse. They occupy all zones of the reef from the very shallow reef flats to deep slopes, feeding on a variety of fauna. Many wrasses also have elaborately patterned bodies and reflect a range of colours from ultraviolet (UV) to far red. As a first step to investigating the visual system of these fishes we measured the transmission properties of the ocular media of 36 species from the Great Barrier Reef, Australia, and Hawaii, Ca

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Bednarz, J. "Effect of three different media on serum free culture of donor corneas and isolated human corneal endothelial cells." British Journal of Ophthalmology 85, no. 12 (2001): 1416–20. http://dx.doi.org/10.1136/bjo.85.12.1416.

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Camposampiero, D., S. Grandesso, E. Zanetti, et al. "Evaluation of the HB&L System for the Microbiological Screening of Storage Medium for Organ-Cultured Corneas." Journal of Ophthalmology 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/670947.

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Aims. To compare HB&L and BACTEC systems for detecting the microorganisms contaminating the corneal storage liquid preserved at 31°C.Methods. Human donor corneas were stored at 4°C followed by preservation at 31°C. Samples of the storage medium were inoculated in BACTEC Peds Plus/F (aerobic microorganisms), BACTEC Plus Anaerobic/F (anaerobic microorganisms), and HB&L bottles. The tests were performed (a) after six days of storage, (b) end of storage, and (c) after 24 hours of preservation in deturgescent liquid sequentially. 10,655 storage and deturgescent media samples were subjected

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Wiffen, Steven J., David O. Hodge, Leif R. Nelson, William E. Bruner, and William M. Bourne. "Lysosomal Enzymes in Corneal Storage Media and Corneal Graft Outcome." Cornea 16, no. 2 (1997): 169???176. http://dx.doi.org/10.1097/00003226-199703000-00009.

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Soni, Nishant G., Caroline K. Hoover, Heather Da Silva, and Bennie H. Jeng. "Preservation of the Corneal Epithelium in Different Corneal Storage Media." Cornea 34, no. 11 (2015): 1400–1403. http://dx.doi.org/10.1097/ico.0000000000000601.

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Songel Sanchis, Bruno, Natalia Sanz Flores, Rafael Bella Gala, and Noemí Güemes Villahoz. "Influencia de la dosis de atropina en el polo anterior de una población infantil miope." Optometría Clínica y Ciencias de la Visión 4, no. 2 (2025): 3–14. https://doi.org/10.71413/4s500m76.

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Relevancia: El empleo del tratamiento farmacológico en forma de colirio de atropina para el control de la progresión de la miopía infantil, puede producir cambios en las diferentes estructuras del polo anterior. Propósito: Evaluar los cambios producidos en el polo anterior en niños miopes en tratamiento con colirio de atropina al 0,025%. Material y Métodos: Se incluyeron niños miopes entre 4 y 16 años en tratamiento con colirio de atropina al 0,025%. Se evaluó la longitud axial, refracción ciclopléjica en equivalente esférico (EE), recuento endotelial (RE) mediante microscopía especular, diáme

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Rinkoski, Tommy A., Cindy K. Bahler, Johann M. Pacheco, et al. "Characterization of a dual media system for culturing primary normal and Fuchs endothelial corneal dystrophy (FECD) endothelial cells." PLOS ONE 16, no. 9 (2021): e0258006. http://dx.doi.org/10.1371/journal.pone.0258006.

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Primary cultures of human corneal endothelial cells (HCECs) are an important model system for studying the pathophysiology of corneal endothelium. The purpose of this study was to identify and validate an optimal primary culture model of normal and Fuchs endothelial corneal dystrophy (FECD) endothelial cells by comparing cell morphology and marker expression under different media conditions to in vivo donor tissues. Primary and immortalized HCECs, isolated from normal and FECD donors, were cultured in proliferation media (Joyce, M4, Bartakova) alone or sequentially with maturation media (F99,

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Jeng, Bennie H. "Preserving the cornea: corneal storage media." Current Opinion in Ophthalmology 17, no. 4 (2006): 332–37. http://dx.doi.org/10.1097/01.icu.0000233950.63853.88.

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Choo, Charlene H., Doug D. Chung, and Anthony J. Aldave. "The Effect of SkQ1 on the Oxidative Stress and Inflammatory Responses in Corneal Endothelial Cells During Ex Vivo Expansion." Eye Banking and Corneal Transplantation 2, no. 4 (2023): e0017. http://dx.doi.org/10.1097/ebct.0000000000000017.

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Purpose: The purpose of this study was to characterize the oxidative stress and inflammatory responses in ex vivo corneal endothelial cells (evCEnC) during expansion and assess the impact of SkQ1, an antioxidant and anti-inflammatory compound, on measures of oxidative stress and inflammation. Methods: A CEnC line (HCEnC-21T) was cultured in media supplemented with varying SkQ1 concentrations to determine the optimal SkQ1 dose range of toxicity and protective effect on CEnC exposed to acute oxidative stress. The impact of SkQ1 treatment on intracellular free radical (IFR) levels, NRF2-mediated

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Schumann, Sandra, Eva Dietrich, Charli Kruse, Salvatore Grisanti, and Mahdy Ranjbar. "Establishment of a Robust and Simple Corneal Organ Culture Model to Monitor Wound Healing." Journal of Clinical Medicine 10, no. 16 (2021): 3486. http://dx.doi.org/10.3390/jcm10163486.

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The use of in vitro systems to investigate the process of corneal wound healing offers the opportunity to reduce animal pain inflicted during in vivo experimentation. This study aimed to establish an easy-to-handle ex vivo organ culture model with porcine corneas for the evaluation and modulation of epithelial wound healing. Cultured free-floating cornea disks with a punch defect were observed by stereomicroscopic photo documentation. We analysed the effects of different cell culture media and investigated the impact of different wound sizes as well as the role of the limbus. Modulation of the

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Cociug, Adrian, Olga Macagonova, Valeriu Jr Cusnir, Vitalie Procopciuc, Valeriu Sr Cusnir, and Viorel Nacu. "The impact of culture media on the endothelial viability of corneas." Moldovan Medical Journal 61(4) (December 15, 2018): 10–14. https://doi.org/10.5281/zenodo.2222291.

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<strong>Background: </strong>The complexity of cryopreservation and its potential to damage the endothelium make it so that it is rarely used in routine eye banking, except for occasional, clinically urgent transplants, where the main objective is to save the eye. The culture medium was the method of choice in the Eye Bank. <strong>Material and methods:</strong> The study group was divided into 3 subgroups. For penetrating keratoplasty and anterior lamellar keratoplasty, corneas taken up to 24 hours, with a number greater than 2000 endothelial cells per mm2, are preferably used. <str

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Slack, James W., Tracy A. Kangas, Henry F. Edelhauser, Dayle H. Geroski, and Mark L. McDermott. "Comparison of Corneal Preservation Media for Corneal Hydration and Stromal Proteoglycan Loss." Cornea 11, no. 3 (1992): 204–10. http://dx.doi.org/10.1097/00003226-199205000-00004.

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Slack, James W., Tracy A. Kangas, Henry F. Edelhauser, Dayle H. Geroski, and Mark L. McDermott. "Comparison of Corneal Preservation Media for Corneal Hydration and Stromal Proteoglycan Loss." Cornea 11, no. 3 (1992): 204–10. http://dx.doi.org/10.1097/00003226-199211030-00004.

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Morgan, Kirk M., William E. Bruner, Richard E. Wyszynski, and Marianne S. DiMarco. "Lysosomal Enzyme Levels in Corneal Storage Media." Ophthalmology 95, no. 11 (1988): 1498–503. http://dx.doi.org/10.1016/s0161-6420(88)32984-2.

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Borzenok, S. A., B. E. Malyugin, D. S. Ostrovskiy, et al. "Survival of the posterior lamellar cornea graft keratocytes and endothelial cells cultivated in the modified corneal preservation media." Fyodorov journal of ophthalmic surgery, no. 2 (July 15, 2021): 32–39. http://dx.doi.org/10.25276/0235-4160-2021-2-32-39.

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Purpose. To study the survival of keratocytes and endothelial cells of a human donor cornea storage in the standard and the new media which was specifically designed for optimized cornea hydration. Material and methods. 2D cell cultures of keratocytes and endothelial cells obtained from the Eye tissue bank were used for culture in improved storage media over a period of 14 and 7 days subsequently. To confirm phenotype characteristics, the cells were stained by the following markers: for keratocytes – Lumikan, Keratocan, and α-smooth muscle actin; for endothelial cells – ZO-1 and Na/K-ATPase. T

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