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1
Forsberg, Tim, and Erik Karlsson. "Comparing Two Production Lines Using Simulation." Thesis, Umeå universitet, Institutionen för matematik och matematisk statistik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-185237.
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In order to reduce material shortage of doors in production, the current production processes needs adjusting. Currently when the buffer between the Mainline and the Door area is full the production in the unit stops. This is a problem that should not happen. To circumvent this a new concept was produced and tested with simulation in order to compare the current state with the new concept model. To achieve this, data has been gathered and analyzed to create a model that is a close approximation of reality. Using the same data input, an alternative solution was tested on two variations of the concept model. The alternative solution showed that the material shortage occurred less frequently in the concept models. The result showed that the material shortage can be drastically reduced if one implements the concept model.
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Walker, Claudio. "Numerical Methods for Two-Phase Flow with Contact Lines." Doctoral thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for energi- og prosessteknikk, 2012. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-17462.
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This thesis focuses on numerical methods for two-phase ows, and especially ows with a moving contact line. Moving contact lines occur where the interface between two uids is in contact with a solid wall. At the location where both uids and the wall meet, the common continuum descriptions for uids are not longer valid, since the dynamics around such a contact line are governed by interactions at the molecular level. Therefore the standart numerical continuum models have to be adjusted to handle moving contact lines. In the main part of the thesis a method to manipulate the position and the velocity of a contact line in a two-phase solver, is described. The Navier-Stokes equations are discretised using an explicit nite di erence method on a staggered grid. The position of the interface is tracked with the level set method and the discontinuities at the interface are treated in a sharp manner with the ghost uid method. The contact line is tracked explicitly and its dynamics can be described by an arbitrary function. The key part of the procedure is to enforce a coupling between the contact line and the Navier-Stokes equations as well as the level set method. Results for di erent contact line models are presented and it is demonstrated that they are in agreement with analytical solutions or results reported in the literature. The presented Navier-Stokes solver is applied as a part in a multiscale method to simulate capillary driven ows. A relation between the contact angle and the contact line velocity is computed by a phase eld model resolving the micro scale dynamics in the region around the contact line. The relation of the microscale model is then used to prescribe the dynamics of the contact line in the macro scale solver. This approach allows to exploit the scale separation between the contact line dynamics and the bulk ow. Therefore coarser meshes can be applied for the macro scale ow solver compared to global phase eld simulations, reducing the overall computational coasts. One of the major drawbacks of the level set method is that it does not conserve the mass of the uids. The application of the conservative level set method (CLSM) o ers a solution to this problem. Three of the attached articles address details concerning the implementation of the CLSM using a nite di erence method. A nite di erence discretisation of the CLSM based on stencils used in the Navier-Stokes solver is described and tested. Various methods to compute the curvature in the CLSM are assessed for the use in the ghost uid method. It is shown that the reinitialisation of the CLSM can lead to spurious deformations of the interface, a stabilised constrained reinitialisation is developed in an attempt to prevent the interface from deforming
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Holmgren, Hanna. "Modelling of Moving Contact Lines in Two-Phase Flows." Doctoral thesis, Uppsala universitet, Avdelningen för beräkningsvetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-329059.
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Moving contact line problems appear in many natural and industrial processes. A contact line is formed where the interface between two immiscible fluids meets a solid wall. Examples from everyday life include raindrops falling on a window and water bugs resting on water surfaces. In many cases the dynamics of the contact line affects the overall behavior of the system. Industrial applications where the contact line behavior is important include gas and oil recovery in porous media, lubrication, inkjet printing and microfluidics. Computer simulations are fundamental tools to understand and predict the behavior. In this thesis we look at numerical simulations of dynamic contact line problems. Despite their importance, the physics of moving contact lines is poorly understood. The standard Navier-Stokes equations together with the conventional no-slip boundary condition predicts a singularity in the shear stresses at the contact line. Atomistic processes at the contact line come into play, and it is necessary to include these processes in the model to resolve the singularity. In the case of capillary driven flows for example, it has been observed that the microscopic contact line dynamics has a large impact on the overall macroscopic flow. In Paper I we present a new multiscale model for numerical simulation of flow of two immiscible and incompressible fluids in the presence of moving contact points (i.e. two-dimensional problems). The paper presents a new boundary methodology based on combining a relation between the apparent contact angle and the contact point velocity, and a similarity solution for Stokes flow at a planar interface (the analytic Huh and Scriven velocity). The relation between the angle and the velocity is determined by performing separate microscopic simulations. The classical Huh and Scriven solution is only valid for flow over flat walls. In Paper II we use perturbation analysis to extend the solution to flow over curved walls. Paper III presents the parallel finite element solver that is used to perform the numerical experiments presented in this thesis. Finally, the new multiscale model (presented in Paper I) is applied to a relevant microfluidic research problem in Paper IV. For this problem it is very important to have a model that accurately takes the atomistic effects at contact lines into account.
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Deon, Jane. "The Lines We Crossed." FIU Digital Commons, 2014. http://digitalcommons.fiu.edu/etd/1150.
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THE LINES WE CROSSED is a historical novel set in Umbria, Italy from 1943-1944. One October morning, Emilia Testadura awakes to find the Nazis have arrived in her village. Major Christoph Strauss presses Emilia into service as housekeeper for the soldiers who now occupy an abandoned palazzo in the village. As the stakes and complications rise in the war throughout winter and spring, so they do for Emilia. Brutal reinforcements arrive and conditions become very dangerous. Emilia realizes she is falling in love with Major Strauss. She learns secrets that change her view of her deceased father and herself. That knowledge leads her to take action which reveals Major Strauss’s true colors before he is sent south to engage the Allies. Once the Allies take Emilia’s village from the Nazis, Emilia makes a final discovery and a decision that leads her south, too, towards a future she had never imagined.
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Bicknell, Gareth Ross. "Biochemical characteristics of apoptosis in two human leukaemic cell lines." Thesis, University of Leicester, 1996. http://hdl.handle.net/2381/34145.
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Apoptosis is a form of cell death in which the cell actively participates. Apoptosis was induced in two human leukaemic cell lines, U937 and HL-60, by incubation with a diverse array of chemical agents. Cell death was assessed by gel electrophoresis, light microscopy and flow cytometry. It was demonstrated that apoptosis involved the formation of large kilobase pair DNA fragments (20-50, 145-245 and 580 kilobase pairs) prior to, or accompanying, internucleosomal cleavage. Degradation of DNA to large kilobase pair sizes also occurred in some forms of necrosis. These fragments were similar, but not identical, to those generated during apoptosis. The identity of the endonuclease(s) responsible for DNA cleavage to large kilobase pair fragments is as yet unknown. One suggestion is that topoisomerase II might be involved. Using an HL-60 subclone with reduced topoisomerase II expression, it was shown that topoisomerase II was not necessary for the formation of large kilobase pair DNA fragments and oligonucleosomal fragments, except where topoisomerase II inhibition was the stimulus for apoptosis. However, topoisomerase II did appear to facilitate 20-50 kilobase pair-to-oligonucleosome conversion, and in one case, it was an absolute requirement for this conversion. Specific proteases have been implicated in the process of apoptosis. The effect of three protease inhibitors was investigated in U937 and HL-60 cells. Aproposed inhibitor of the protease, calpain, had little effect. In contrast, two serine protease inhibitors inhibited the formation of oligonucleosomal fragments. One of these also inhibited large kilobase pair fragments. The other induced apoptotic fragmentation of DNA to large kilobase pair fragments. The results suggest that different proteases may suppress or promote apoptosis within the same cell.
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Shi, Xuesong. "Synthesis and characterization of two azobenzene-containing ferroelectric liquid crystals and of their corresponding polysiloxanes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ63365.pdf.
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Jordán, Vargas Kathia. "Feed conversion index in two populations and two lines of guinea pigs for meat production." BYU ScholarsArchive, 2005. https://scholarsarchive.byu.edu/etd/5381.
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The guinea pig (Cavia aperea porcellus) is an alternative to improve human nutrition because its meat is of excellent taste and quality. Thus, it is an important nutritional source. The feed conversion index was determined under the basic (forage) and mixed (forage and concentrate) diets during the growth phase. This was done with both sexes located in the Tamborada and MEJOCUY populations, using the AUQUI, and San Luis lines. This was done in order to quantify how many kilograms of feed an animal must eat to gain one kilogram of live weight. The animals were randomly distributed into individual pools based on population, line, and sex for the period from 14 to 56 days old. Depending on the feeding system used, they received alfalfa forage and/or concentrated feed. In addition, they were weighed before and after feeding so the difference between food eaten and food rejected could be calculated. At the end of the 42 days of investigation, the feed conversion indexes were 5, 5.1, 4.8, and 4.6 for the basic diet and 5, 5.5, 4.9, and 4.9 for the mixed diet for the guinea pigs of the Tamborada and MEJOCUY populations and the AUQUI and San Luis lines respectively. The male and female animals had indexes of 4.7 and 5.3 respectively. Generally speaking, the San Luis line has the best feed conversion index, followed by the AUQUI line, the Tamborada population, and lastly the MEJOCUY population.
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Schnablegger, Gerald. "The anticancer activity of Cyathula prostrata on two malignant cell lines." Thesis, Nelson Mandela Metropolitan University, 2010. http://hdl.handle.net/10948/1563.
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Plants have always been a source of medicine and are still being used by traditional healers in the rural part of Africa, Asia and India to treat a range of illnesses including cancer. The in vitro anticancer activity of an 80 percent ethanol extract of Cyathula prostrata, an annual branching shrub used by traditional healers in Nigeria to treat cancer was investigated. No previous studies have outlined the possible pathways and mechanisms used by cancer cells when treated with C. prostrata. Dose response analysis was performed to determine the effective cytotoxic concentrations of C. prostrata on HeLa (cervical cancer cell line) and U937 (myelo-monocytic cell line). The IC50 values were 100.8 μg/ml and 64.4 μg/ml for HeLa and U937 cells, respectively. All further experiments were performed using 125 μg/ml C. prostrata extract and 50 μM cisplatin as positive control. With the use of the fluorescent DNA binding dye propidium iodide, the induction of tumour cell death by C. prostrata extract has been linked to cell cycle arrest in the G1 phase at 24 and 48 h. In both cell lines, more than 80 percent of the C. prostrata treated cells were found in the G1 phase after 48 hours of treatment. The annexin V-FITC/PI assay revealed an increase in the percentage apoptotic cells from 4.9 percent to 53.1 percent at 24 h and 8.3 percent to 50.3 percent at 48 h. Since apoptosis induction can occur via a number of different pathways, distinct features were used as markers to investigate the mode of action of this C. prostrata extract. Markers such as activated caspase-8, p21 and cyt-c, were investigated with the aid of fluorescently labelled (FITC) antibodies with analysis using flow cytometry. No change in p21 levels was observed in response to treatment with the extract for up to 48 h. Cell cycle arrest in G1 was therefore not induced by this cyclin-CDK inhibitor. Increase in caspase-8 activation was observed in response to treatment with the extract with no cyt-c release from the mitochondria. The lack of cyt-c release was due to no change in mitochondrial membrane potential, which was investigated with the aid of fluorescent mitochondrial dyes and flow cytometric techniques. Caspase-8 activation is unique to the extrinsic apoptotic pathway. The results from this study therefore show that C. prostrata extract induces apoptosis via the extrinsic pathway and that this activation in independent of the mitochondria. The levels of hTERT, the catalytic subunit of telomerase, were investigated as an additional molecular target for C. prostrata. This was also investigated using FITC labelled antibodies and flow cytometry. A decrease in hTERT levels was observed following C. prostrata treatment. The findings from this study suggest that the extract acts through multiple targets, by inducing: cell cycle arrest in the G1 phase through an unknown mechanism; apoptosis through an extrinsic death receptor pathway and replicative senescence through inhibition of telomerase.
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Paisley, Derek John. "Characterisation of two mouse mutant lines generated by transgene insertional mutagenesis." Thesis, University of Bath, 2000. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342073.
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Grabowsky, Monika Elvira. "Two-dimensional chromatographic characterisation of PS-b-PEO copolymers at the critical conditions of their corresponding homopolymers." Thesis, Stellenbosch : Stellenbosch University, 2011. http://hdl.handle.net/10019.1/17937.
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Thesis (MSc)--Stellenbosch University, 2011.<br>ENGLISH ABSTRACT: Block copolymers are very interesting materials but they are quite complex. During polymer
synthesis only a certain amount of control can be enforced. As copolymers are made up of two
or more different homopolymer segments, and therefore have different end group possibilities,
varying block lengths and block sequences, they have complex structures and are therefore
difficult to analyse.
Different techniques exist by which polymers can be analysed to determine the
aforementioned distributions. In order to achieve a complete characterisation of a polymer
structure, it is best to first use a separation technique to fractionate the polymer into more
homogeneous fractions, and then use identification techniques to analyse these fractions.
Polystyrene-block-poly(ethylene oxide) (PS-b-PEO) copolymers were investigated using
liquid chromatography at the critical conditions (LCCC) of the copolymers' corresponding
homopolymers, two-dimensional liquid chromatography (2D-LC) and FTIR. The block
copolymers were analysed using the established LCCC of PS but it was found that even
though separation of PS homopolymer and copolymer was obtained, PS blocks of the
copolymers contributed to some extent to the retention of the PEO blocks.
Some of the block copolymer samples were fractionated at the established critical
conditions of PS. These fractions were qualitatively and quantitatively analysed using FTIR
spectroscopy. The settings for the 2D-LC analysis were established, using LCCC of PS as the
first dimension and as the second dimension SEC, using DMF as eluent. DMF was a suitable
solvent to be used for the second dimension because PS, PEO and PS-b-PEO exhibited good
solubility in this solvent. THF did not dissolve the block copolymers completely.
The same solvent system as used for LCCC of PS was used for LCCC of PEO, but the
critical conditions correspond to a different solvent composition. The block copolymers were
analysed using the established LCCC of PEO but it was found that even though separation of
PEO homopolymer and copolymer was obtained, the PEO blocks of the copolymers
contributed to some extent to the retention of the PS blocks. Some of the block copolymer
samples were fractionated at the established critical conditions of PEO. These fractions were
qualitatively and quantitatively analysed using FTIR spectroscopy. The settings for the 2D-LC
analysis were established, using LCCC of PEO as the first dimension and as the second
dimension SEC using DMF as eluent was used. Lastly, qualitative and quantitative analyses of
the block copolymers were carried out using FTIR spectroscopy.<br>AFRIKAANSE OPSOMMING: Alhoewel blokkopolimere baie interessante verbindings is, is hulle redelik ingewikkeld.
Gedurende die kopolimerisasiereaksie kan daar net 'n sekere mate van kontrole behaal word.
Aangesien kopolimere uit twee of meer homopolimeersegmente, met verskillende end-groep
moontlikhede, bloklengtes en blokvolgordes bestaan, is dit baie moeilik om hierdie verbindings
te analiseer.
Verskillende tegnieke kan gebruik word vir die analise van polimere en die bepaling van
bogenoemde verspreidings. Ten einde 'n polimeerstruktuur volledig te karakteriseer is die beste
manier om eers 'n skeidingstegniek te gebruik om die polimeer in meer homogene fraksies te
fraksioneer en dan daarna hierdie fraksies te analiseer.
Polistireen-blok-poli(etileenoksied) (PS-b-PEO) kopolimere is ondersoek deur gebruik te
maak van vloeistofchromatografie by kritiese kondisies (LCCC) van die kopolimeer se
ooreenkomstige homopolimere; twee-dimensionele vloeistofchromatografie (2D-LC) en FTIR.
Die blokkopolimere is gekarakteriseer deur gebuik te maak van bevestigde LCCC van PS. Daar
is egter gevind dat alhoewel skeiding van die PS homopolimeer en die kopolimeer behaal is, PS
blokke van die kopolimere in 'n mate bygedra het tot die retensie van die PEO blokke.
Sommige van die blok-kopolimeermonsters is gefraksioneer by die bepaalde kritiese
kondisies van PS. Hierdie fraksies is kwalitatief en kwantitatief geanaliseer deur gebruik te maak
van FTIR spektroskopie. Die stellings vir die 2D-LC analise is bepaal deur gebruik te maak van
LCCC van PS as die eerste dimensie en SEC as die tweede dimensie, met DMF as elueermiddel.
DMF was 'n geskikte oplosmiddel vir die tweede dimensie aangesien PS, PEO en PS-b-PEO
goed oplosbaar is daarin. Die blokkopolimere was nie volledig oplosbaar in THF nie.
Dieselfde oplosmiddelsisteem soos gebruik vir die LCCC van PS is gebruik vir die
LCCC van PEO, maar die kritiese kondisies stem ooreen met 'n ander oplosmiddelsamestelling.
Die blokkopolimere is geanaliseer deur gebruik te maak van die bevestigde LCCC van PEO,
maar daar is bevind dat alhoewel skeiding van die PEO homopolimeer en kopolimeer behaal is,
die PEO blokke van die kopolimere in 'n mate bygedra het tot die retensie van die PS blokke.
Sommige van die blokkopolimeermonsters is gefraksioneer by die bevestigde kritiese kondisies
van PEO. Hierdie fraksies is kwalitatief en kwantitatief geanaliseer deur gebruik te maak van
FTIR spektroskopie. Die stellings vir die 2D-LC analise is bepaal deur gebruik te maak van LCCC van PEO as die eerste dimensie en SEC as die tweede dimensie, met DMF as
elueermiddel. Laastens is kwalitatiewe en kwanitatiewe analises van die blokkopolimere m.b.v.
FTIR spektroskopie uitgevoer.
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Ruddy, Suzanne. "Mutation of the thymidine kinase gene in two mouse tumour cell lines." Thesis, Queen's University Belfast, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336001.
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Jones, Felicity Anne. "Growth and voluntary feed intake of two diverse genetic lines of pigs." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284424.
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Solymosi, Bence. "A two-way approach to adapt small-scale laboratory experiments and corresponding numerical simulations of offshore seismic surveys." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0630.
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Les méthodes numériques sont largement utilisées en exploration sismique pour simuler la propagation des ondes et pour le post-traitement des données sismiques avant l'interprétation géologique/géophysique. Les algorithmes sont basés sur différentes hypothèses pour réduire le coût de calcul au détriment de la simplification des modèles et/ou des phénomènes physiques. En raison de leur rôle essentiel en exploration géophysique, la précision des simulations numériques présente un fort intérêt, notamment dans le cas de configurations géologiques réalistes. La comparaison directe des résultats numériques entre eux dans des configurations synthétiques peut avoir des limites, car il peut être difficile de déterminer celui qui donne la meilleure approximation de la solution physique inconnue. Comme dans la réalité le sous-sol n'est jamais connu avec précision, il est également difficile de comparer les résultats synthétiques aux données sismiques réelles acquises in situ. Par conséquent, il y a un grand intérêt à utiliser des mesures de laboratoire sur des modèles physiques aux propriétés connues pour valider la précision des outils numériques. Avant de pouvoir comparer avec précision les mesures et les simulations, nous devons tout d’abord établir un cadre comparatif avec une approche conjointe adaptée aux expériences de laboratoire et à la modélisation numérique. C’est précisément l'objectif de cette thèse. Ainsi, le cadre reproduit d'abord les mesures sismiques marines dans des conditions de laboratoire en utilisant de modèles à échelle réduite, puis les outils numériques sont adaptés à la reconstruction précise des expériences<br>Numerical methods are widely used in seismic exploration to simulate wave propagation and to post-process the recorded seismic data before the geologic/geophysical interpretation. The algorithms are based on various assumptions to reduce the computational cost at the expense of simplifying the models and/or the physical phenomena. Because of their essential role in exploration geophysics, the accuracy of the numerical simulations is of particular interest, especially in the case of realistic geologic setups. The direct comparison of the numerical results with each other in synthetic configurations can have limitations, as it can be difficult to determine the one that gives the best approximation of a physically unknown solution. Because in real life the subsurface is never accurately known, it is also difficult to compare the synthetic results to any seismic data set from field measurements. Therefore there is a strong interest in using laboratory measurements on physical models of known geometries to benchmark the numerical tools. Before comparing measurements and simulations with confidence at high accuracy, we first need to establish a comparative framework with a jointly-adapted approach to both the laboratory experiments and the numerical modeling. This challenging task is the goal of this thesis. Thus, the framework first reproduces offshore seismic measurements in laboratory conditions with the help of small-scale models, and then the numerical tools are adapted to the accurate synthetic reconstruction of the experiments
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Payette, Paul J. "Characterization of two Kaposi's sarcoma cell lines and their response to chemotherapeutic agents." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0010/MQ28453.pdf.
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Healy, Caroline Marie Therese. "Flow cytometric detection of chemically induced tandem repeat mutations in two murine cell lines." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/26920.
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To facilitate detection of genotoxicity from environmental mutagen exposure we generated an in vitro green fluorescence protein (GFP) activation assay that quickly and effectively detects frameshift mutations in tandem repeat sequences (TRS). Two murine cell lines were stably transfected with GFP reporter plasmids in which the GFP constructs contain TRS that shift the GFP coding sequence out of frame. These included several 2-6bp repeat sequences, a control non-repetitive sequence and a human gene sequence with TRS. Transfected cultures were exposed to five model chemicals: hydrogen peroxide (H2O2), 12-O-tetradecanoyl-phorbol-13-acetate (TPA), benzo-a-pyrene-diol-epoxide (BPDE), ethyl nitrosourea (ENU), 9-aminoacridine (9AA). Frameshift mutations resulted in green fluorescent revertants, as determined by flow cytometry and confirmed by sequencing. All five treatments induced a statistically significant sequence- and dose-dependent response in both cell lines. Results from these experiments reveal that the assay responds robustly to various classes of mutagenic substances, as well as carcinogens that are inactive in conventional mutation assays.
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Towey, Michael Paul. "Characterisation of the genetic defects in two novel MHC class II deficient cell lines." Thesis, King's College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270493.
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GOMES, VITOR DE CASTRO. "GENETIC SELECTION OF TWO NEW RAT LINES DISPLAYING DIFFERENT LEVELS OF CONDITIONED FREEZING BEHAVIOR." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2012. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=28977@1.
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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO<br>CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO<br>Criação seletiva bidirecional de uma resposta defensiva ou qualquer outra característica fenotípica é uma técnica na qual animais são criados com o objetivo de modificar a frequência dos genes que estão subjacentes a um fenótipo em particular. O acasalamento de animais de uma determinada população com base nos extremos opostos de uma característica observável vai propagar, após diversas gerações, este fenótipo particular em direções opostas, levando-se à criação de duas linhagens contrastantes. No presente trabalho empregamos o congelamento condicionado em resposta a estímulos contextuais previamente associados com choques elétricos nas patas como critério de seleção para o desenvolvimento de duas novas linhagens de ratos. O protocolo básico consistiu de acasalamento entre machos e fêmeas Wistar com as maiores e as menores taxas de congelamento condicionado em resposta a sinais contextuais da câmara experimental onde os animais foram expostos a três choques elétricos não sinalizados no dia anterior. O Estudo 1 apresenta os resultados iniciais de quatorze gerações de criação seletiva. Os resultados mostraram que diferenças significativas entre estas duas linhagens foram encontradas após 3 gerações, indicando um forte componente hereditário deste tipo de aprendizagem. As linhagens foram denominadas Cariocas com Alto Congelamento Condicionado (CAC) e Cariocas com Baixo Congelamento condicionado (CBC). Além disso, nós introduzimos um terceiro grupo de animais aleatoriamente selecionados (CTRL) em nosso programa de criação seletiva. No Estudo 2 investigamos os diferentes padrões de extinção e da reaquisição do medo condicionado nestas duas novas linhagens. Por fim, no Estudo 3, nossos resultados sugeriram uma dissociação entre o medo contextual e o medo discreto entre animais CAC e CBC.<br>Bidirectional selective breeding of a defensive response or any other phenotypic characteristic is a technique in which animals are bred to modify the frequency of the genes that underlie a particular phenotype. Mating animals within a population based on the opposite extremes of an observable characteristic will push, over many generations, this particular phenotype in opposite directions, leading to two separately bred lines. In the present work we employed the conditioned freezing in response to contextual cues previously associated with footshock as the phenotype criterion for developing two new rat lines. The basic protocol consisted of mating male and female albino Wistar rats with the highest and lowest conditioned freezing in response to the contextual cues of the experimental chamber where animals were exposed to three unsignaled electric footshocks on the previous day. Study 1 presents the initial results of fourteen generations of selective breeding. We found that after three generations, reliable differences between these two lines were already present, indicating a strong heritable component of this type of learning. The lines were named Carioca High conditioned Freezing (CHF) and Carioca Low conditioned Freezing (CLF). Also, we introduced a third group of randomly selected animals (RND) in our selective breeding program. In Study 2, we investigated the different patterns of fear extinction and reacquisition in these two new lines. Finally, in Study 3, results showed dissociation between contextual and phasic fear between CHF and CLF rats.
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Bütler, Timo Martin. "Analysis of phosphotyrosyl protein phosphatases in two human cell lines and in rat tissues." [S.l : s.n.], 1989. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
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Mazzoni, Luca. "In vitro effects of Alba strawberry cultivars on two murine breast cancer cell lines." Doctoral thesis, Università Politecnica delle Marche, 2015. http://hdl.handle.net/11566/243006.
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L’obiettivo principale degli studi condotti durante il corso di dottorato è stato quello di valutare gli effetti del trattamento con estratti di fragola su 2 modelli murini in vitro di cancro al seno, cercando di verificare se nel meccanismo di azione tramite il quale le fragole sembrano svolgere un’azione antitumorale sia implicato l’AMPK. Nella prima parte degli studi sono state valutate le caratteristiche sensoriali (solidi solubili e acidità totale) e nutrizionali (contenuto totale di fenoli, di flavonoidi, di antociani, di vitamina C e di folati) e la capacità antiossidante di 5 diverse cultivar commerciali di fragola. Dopo averle valutate, la scelta per effettuare i test in vitro è ricaduta su Alba, per il suo valore nutrizionale.
Nella seconda parte del dottorato è stata valutata la capacità antitumorale degli estratti metanolici ed antocianici della cultivar Alba su 2 linee tumorali murine di cancro al seno, N202/1A (alta espressione dell’oncogene HER2/neu) e N202/1E (bassa espressione dell’oncogene HER2/neu), dopo 48 e 72 ore di trattamento. In particolare, sono stati determinati il tasso di vitalità, di apoptosi e di ROS intracellulari. Il danno alle cellule tumorali è stato valutato anche tramite l’analisi della funzionalità mitocondriale (capacità di glicolizzare e di consumare ossigeno) e lo stato ossidativo (misura dell’attività della SOD e della CAT).
Infine, è stato usato il Western Blot per l’analisi dell’espressione di proteine correlate all’apoptosi, all’autofagia, alle metastasi, allo stato ossidativo, alla funzionalità mitocondriale, all’espressione dell’oncogene e alla regolazione dell’AMPK. A questo proposito, le cellule sono state anche trattate con il Composto C, un inibitore dell’AMPK, e sono stati valutati i suoi effetti sulla vitalità cellulare, l’apoptosi e l’espressione dell’AMPK rispetto a quelli in presenza di un trattamento con estratti di fragola.
Questo studio ha dimostrato che fragole ben caratterizzate possiedono un effetto antiproliferativo su cellule tumorali, tramite l’induzione di apoptosi e di stress ossidativo. Allo stesso tempo, questo lavoro approfondisce le conoscenze sul possibile meccanismo di azione, spiegando in parte l’effetto delle fragole su cellule tumorali e dimostrando l’esistenza di una relazione tra la via dell’AMPK e l’effetto anticancerogeno delle fragole stesse.<br>The general aim of this PhD course was the evaluation of the effects of strawberry extracts treatment on two in vitro models of murine breast cancer cell line, trying to detect a specific pathway (AMPK) through which strawberries exert their anticancer activity. In the first part of the PhD course 5 different strawberry commercial cultivars, 4 of them resulting from the UNIVPM breeding program, were evaluated for their sensorial and nutritional qualities, in particular soluble solids and total acidity. Regarding the nutritional qualities, the content of total phenolics, flavonoids, anthocyanins, vitamin C and folates were evaluated. The antioxidant capacity was also measured, through 3 different methodologies. After the evaluation of the 5 different cultivars, Alba was chosen for its nutritional value and was used for the second part of PhD course. In this second part, the anticancer activity of methanolic and anthocyanin purified extracts from Alba cultivar on two murine cancer cell lines, N202/1A (with high levels of HER2/neu oncogene) and N202/1E (with low levels of HER2/neu oncogene) were evaluated after 48 and 72 h of treatment. In particular, the viability, apoptosis and intracellular ROS rates were assessed. The cell damage was also estimated through the evaluation of the mitochondrial functionality (respiratory and glycolitic assay) and the oxidative status (measurement of SOD and CAT activity). Finally, Western Blot assays were performed to analyze the expression of several proteins related to apoptosis, autophagy, metastasis, oxidative status, mitochondrial functionality, oncogene expression and AMPK pathway. On this regard, cells were also treated with Compound C, an AMPK inhibitor, and the effects of this compound on cell viability, apoptosis and AMPK expression were assessed, in order to compare them with the results obtained after the strawberry treatment.
This study demonstrated that a well characterized strawberry possessed an antiproliferative effect on cancer cells, through the induction of apoptosis and oxidative stress. At the same time, this study is one of the first that have tried to in deep a candidate pathway for the explanation of the strawberry effects on cancer cells, and as a results a relation between the AMPK pathway and the anticancer effects of strawberries was demonstrated.
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Stylin, Nicole. "Mechanism specific effects of two organic pollutants in a single and co-cultured system using two cell lines from Rainbow trout." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-86195.
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The present project aims at the investigation of the toxic effects of two organic environmental compounds and to determine whether the interactions of the compounds are different in a single and co-cultured system using two cell lines. The two compounds used for this purpose were PFBS (perfluorobutanesulfonic acid) and BaP (benzo[a]pyrene). For this study, two cell lines from rainbow trout, RTgill-W1 (gills) and RTL-W1 (liver) were used to test the toxic impacts of the selected compounds. The cell lines were cultured before performing the further tests using Neutral red cytotoxicity assay, qPCR and a multiple cell system. Neutral red was used to test acute toxicity, qPCR to determine changes in the expression of selected genes, and the multiple cell system for co-culturing. The hypothesis was to establish if there where interactions between the two cell lines in co-culturing and to investigate their sensitivity towards the compounds was comparable to single cell line. Neutral red indicated that neither BaP, PFBS or mixed compounds was causing acute toxicity. However, expression in gene regulation varied between the tested concentrations of BaP and PFBS, thus; co-culture indicated on lower expression.
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Abbott, Heather Elizabeth. "Comparisons of the factors influencing intrinsic radiosensitivity between two isogenic human ovarian carcinoma cell lines." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0021/MQ57080.pdf.
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Sondergaard, Jeffrey, Kyle Rice, Kira Weaver, Devin Josey, Taylor Gregory, and Christie Monahan. "Comparison Of Two Rat Somatotroph Cell Lines To Examine Tissue-Specific Transcription Of Growth Hormone." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/asrf/2018/schedule/185.
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Growth Hormone (GH), also known as somatotrophin, is of cardinal importance in the regulation of somatic growth. Decreased GH expression results in short stature, whereas increased levels of GH lead to disorders such as gigantism or acromegaly. Mutations in the transcription factor Pit-1 have been shown to decrease GH, as well as prolactin (PRL; Parks et al., JCEM, 1999). However, understanding the regulation by selective GH transcription regulators, such as Zn16, a protein encoding 16 zinc fingers that binds to the GH promotor DNA (Wojtkiewicz et al., Endocrine, 2002), will require comparison of currently available rodent cell lines that express GH. Two current rat somatotroph cell lines are the somatotroph MtT/S and lactosomatotroph GH3 cell lines, both of which have been implemented in studies on the regulation of GH expression (Schaaf et al., Endocr Relat Cancer, 2009). MtT/S cells almost exclusively express GH, whereas GH3 cells are less differentiated and co-express PRL as well as GH. GH3 cells have been available longer, and thus are more frequently used for in vitro GH experiments, but there may be some increased utility in utilizing MtT/S cells. MtT/S cells were procured from Riken Cell Bank in Japan, and GH3 cells were acquired from the ATCC. These lines were cultured, then secretion of GH and PRL was examined after Insulin-like Growth Factor (IGF-1) treatment, which is inhibitory to GH and PRL secretion. Further, both cell lines were treated with stimulatory factors GH releasing hormone (GHRH); retinoic acid; cortisone; GH Releasing Peptide-6; and both cortisone and GHRH. GH secretion was on average higher in MtT/S cells than in GH3 cells. On the other hand, PRL secretion was extremely lower in MtT/S cells than in GH3 cells. This result confirms that the MtT/S cells are further differentiated as somatotrophs than GH3 cells. Although hormone release in response to treatment did not appear different, the overall difference in GH vs. PRL secretion may be useful for evaluating the role of Zn16 in selective control of the GH promoter. Therefore, mRNA levels of GH, PRL, Pit-1 and Zn16 in these cell lines are currently being measured using quantitative real-time PCR with ribosomal protein L19 (RPL19) expression as a standard. With the investigation of the characteristics of these differentiated pituitary cell types, we hope to advance the knowledge of GH transcription and regulation, particularly the study of Zn16 effects during pituitary development. Recently it was observed that Zn16 may have a role in a tumor suppressor gene that undergoes mutation leading to a form of colorectal cancer and childhood leukemia. This augments the utility of determining the proper cell lines to examine Zn16, and its role in tumor suppression and cell division as well as gene expression during hypophyseal development.
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Chen, Yiwen. "Genome-wide association studies on body weight and component traits in an intercross of two divergently selected chicken lines." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 1997. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-453443.
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Here we present the genome-wide association study of body weight at 8 weeks of age based onthe advanced intercross pedigree of two chicken lines gone through bi-directional selection.With improved marker density (~3M SNPs) and larger sample size (2667 individuals from F2-F15), 34 loci with suggestive significance are detected, of which 18 loci are novel, and the rest17 loci are consistent with the results of previous quantitative trait locus mapping studies onthis trait with smaller number of genetic markers and fewer individuals. The component traits,referring to traits related to body weight and possibly contributing to the body weight as well,are also measured and analysed. The combined result showed that one locus with significantmarginal effect on BW8 is associated with early growth, breast muscle development and shankdevelopment, while another locus with late development and bursa development.
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Ong, Colin Heng Piew. "Biochemical properties of two bovine polynucleotide kinases and a homology-based polymerase chain reaction strategy for identification of the corresponding genes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0004/MQ44237.pdf.
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Ong, Colin Heng Piew. "Biochemical properties of two bovine polynucleotide kinases and a homology-based polymerase chain reaction strategy for identification of the corresponding genes." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=20603.
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Four separate polynucleotide kinase activities were observed following Polymin P precipitation of crude extract of calf thymus tissue (Prinos et al., 1995). A single activity designated Polymin P-Precipitable Polynucleotide Kinase (PP-PNK) was detected in the pellet fraction. The other three separable activities, designated SNQl, SNQII and SNQIII, were found in the supernatant fraction after chromatography on a Q-Sepharose column.<br>The PP-PNK activity was purified 4,200 fold after 6 purification steps. The PP-PNK preparation preferentially used ATP as a gamma-phosphoryl donor compared with GTP. Chromatography on a Sephadex G-150 column demonstrated that it had a molecular weight of 130 kDa. Immunoblot analysis using an anti-peptide antibody raised against a purified fetal bovine polynucleotide kinase revealed no signal in the PP-PNK preparation suggesting that PP-PNK is distinct from SNQI and fetal calf thymus DNA kinase-phosphatase.<br>Biochemical analyses of SNQIII preparation revealed a pH optimum of 5.5 to 6.0 and required Mg2+ for activity. Studies with different oligonucleotide substrates showed that for PP-PNK, the activity profile was: dT20 > rA20 > dA20 whereas for SNQI, it was as follows: dA20 > dT20 > rA20. Both SNQIIIa and SNQIIIb samples had the same profile: dA20 ≈ dT20 > rA20.<br>A homology based PCR approach to isolate cDNAs encoding mammalian PNKs was developed. To date, no positive clones have been identified using human thymus and a human lymphoblast cDNA library as template DNA.
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Walker, Paul. "Cloning and characterisation of DNA flanking highly expressed integrated plasmids in two mouse myeloma cell lines." Thesis, University of Leicester, 1999. http://hdl.handle.net/2381/29640.
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Attempts to optimise plasmid vectors for the high level expression of heterologous proteins in the mouse myeloma cell line, J558L, led to the chance discovery of two highly expressing transfectants (C6 and D8). Both these transfectants resulted from plasmids containing a gpt selection marker, a lysozyme reporter gene and the IgH enhancer. Both expressed the lysozyme reporter at around 100 fold higher level than the normally obtained from transfection with the same plasmids, and approximately the same level as the endogenous immunoglobulin genes. This thesis describes the cloning and characterisation of DNA flanking the plasmids in both the C6 and D8 cell lines. A genomic library for the C6 cell line was constructed in -DASH and screened with plasmid sequence probes. A single clone of 15kb was isolated. This contained 9kb of flanking DNA from one side of the plasmid. Sequencing of the clone revealed that the plasmid had integrated within a B1 repeat element. No homologies to other known sequences were identified. Similarly, a genomic library from the D8 cell line was constructed and screened. Three independent clones were obtained giving a total of 20kb flanking DNA. Sequencing revealed that flanking DNA on one side of the plasmid was extremely A/T rich. The possible implications of this are discussed. The A/T rich fragment from the D8 locus was shown to bind to isolated nuclear matrix in vitro. However no binding to the nuclear matrix was detected for either locus in an in vivo assay. Both the C6 and D8 loci were shown to be DNase sensitive, but no specific hypersensitive sites were identified for either cell line. Also presented is work which contributes to a project aimed towards using Flp recombinase to place expression constructs and other DNA sequences at the C6 locus by homologous recombination.
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Kelly, Janette Boyd. "Cellular mechanisms in the development of secondary cataract : characterisation of two mammalian lens epithelial cell lines." Thesis, University of the West of Scotland, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.427715.
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Harris, Stephen. "The characterisation of two enhancer trap lines expressed in the embryonic nervous system of Drosophila melanogaster." Thesis, University of Warwick, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282431.
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Fukuyama, Keita. "Gene expression profiles of liver cancer cell lines reveal two hepatocyte-like and fibroblast-like clusters." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/265181.
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Kucukkoc, Ibrahim. "Modelling and solving mixed-model parallel two-sided assembly line problems." Thesis, University of Exeter, 2015. http://hdl.handle.net/10871/18917.
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The global competitive environment and the growing demand for personalised products have increased the interest of companies in producing similar product models on the same assembly line. Companies are forced to make significant structural changes to rapidly respond to diversified demands and convert their existing single-model lines into mixed-model lines in order to avoid unnecessary new line construction cost for each new product model. Mixed-model assembly lines play a key role in increasing productivity without compromising quality for manufacturing enterprises. The literature is extensive on assembling small-sized products in an intermixed sequence and assembling large-sized products in large volumes on single-model lines. However, a mixed-model parallel two-sided line system, where two or more similar products or similar models of a large-sized product are assembled on each of the parallel two-sided lines in an intermixed sequence, has not been of interest to academia so far. Moreover, taking model sequencing problem into consideration on a mixed-model parallel two-sided line system is a novel research topic in this domain. Within this context, the problem of simultaneous balancing and sequencing of mixed-model parallel two-sided lines is defined and described using illustrative examples for the first time in the literature. The mathematical model of the problem is also developed to exhibit the main characteristics of the problem and to explore the logic underlying the algorithms developed. The benefits of utilising multi-line stations between two adjacent lines are discussed and numerical examples are provided. An agent-based ant colony optimisation algorithm (called ABACO) is developed to obtain a generic solution that conforms to any model sequence and it is enhanced step-by-step to increase the quality of the solutions obtained. Then, the algorithm is modified with the integration of a model sequencing procedure (where the modified version is called ABACO/S) to balance lines by tracking the product model changes on each workstation in a complex production environment where each of the parallel lines may a have different cycle time. Finally, a genetic algorithm based model sequencing mechanism is integrated to the algorithm to increase the robustness of the obtained solutions. Computational tests are performed using test cases to observe the performances of the developed algorithms. Statistical tests are conducted through obtained results and test results establish that balancing mixed-model parallel two-sided lines together has a significant effect on the sought performance measures (a weighted summation of line length and the number of workstations) in comparison with balancing those lines separately. Another important finding of the research is that considering model sequencing problem along with the line balancing problem helps algorithm find better line balances with better performance measures. The results also indicate that the developed ABACO and ABACO/S algorithms outperform other test heuristics commonly used in the literature in solving various line balancing problems; and integrating a genetic algorithm based model sequencing mechanism into ABACO/S helps the algorithm find better solutions with less amount of computational effort.
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Shukla, Shaleen. "Calculation of Nuclear Level Densities Near the Drip Lines." Ohio University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1209754262.
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Leggio, Loredana. "Functional analysis of two alternative transcripts from porin1 gene of Drosophila melanogaster and involvement of corresponding 5'UTR sequences in the translation control." Doctoral thesis, Università di Catania, 2017. http://hdl.handle.net/10761/3935.
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VDAC (Voltage Dependent Anion-selective channel) is a voltage-dependent anion selective channel, a pore forming protein located in the outer mitochondrial membrane (OMM) of all eukaryotic organisms. This protein allows the passage of nucleotides, ions and small metabolites between cytosol and mitochondria. VDAC has a beta-barrel structure with its N-terminal forming an a-helix inserted into the pore and involved in the gating process. VDAC takes a maximum open state at voltage around 0mV; while at voltages greater than 20mV, for positive and negative values, VDAC switches to a closed state. The crucial role of this channel is dictated by its strategic position, able to interact with many enzymes, proteins or metabolites directly or indirectly involved in several cellular pathways, explaining thus its involvement in many diseases. In Drosophila melanogaster the gene encoding for the principal isoform of VDAC is porin1,which is made up by five exons, of which exon 1A and exon 1B, being 5 UTR sequences, are alternative between them. In fact, by means an alternative splicing process two transcripts are produced containing at the 5 -end or the exon 1A or the exon 1B, followed by the same coding sequence. The alternative transcripts 1A-VDAC and 1B-VDAC are produced in all developmental stage of fly and in any tissue. Thanks to a previous work from our team we know that 1B-VDAC transcript is unproductive because it is not translated. This result allowed us to speculate about a different cellular function for this 1B-VDAC transcript, respect the canonical 1A-VDAC mRNA.
Considering all data known, the main objectives of my thesis work were: 1) understanding the molecular mechanisms responsible of the failing of 1B-VDAC mRNA translation; 2) investigate about the meaning in D. melanogaster of the alternative unproductive 1B-VDAC mRNA. Using several organism models, such as Saccharomyces cerevisiae Dpor1, HeLa cells and Drosophila melanogaster embryonic SL2 cell, we obtained important results that allow us to formulate the following hypothesis: the inhibitor role played by the 5 UTR 1B sequence on translation in yeast is probably associated to the action of specific RBPs able to bind the inner sequence 16-31. In yeast this mechanism is itself sufficient to guarantee the translational repression of the coding sequence of a gene reporter as well as the full-length mRNA of porin1 gene, demonstrating in this way that the 5 UTR 1B contains all necessary information for inducing inhibition of protein synthesis in yeast; in Drosophila the 3 UTR sequence of 1B-VDAC transcript is indispensable for carry out the translational repression mechanism of the same transcript. In fly indeed, the 1B-Luc construct is never expressed while the same 5 UTR-1B fused to the porin1 coding sequence does not influence translation of the same porin; the 5 UTR 1A represents in general a sequence able to amplify translation of any coding sequence fused to it. Indeed, fusing the 5 UTR 1A with coding sequences of gene reporters we obtained always a noticeable increase in the expression of the relative protein. This effect is not detectable in fly cells where, after transfection with the heterologous transcript 1A-porin, an increase of the endogenous amount of VDAC protein is not obtained. In Drosophila the 3 UTR sequence of 1A-VDAC transcript plays probably a role in controlling endogenous levels of VDAC. Indeed, by transfecting fly cells with the 1A-VDAC transcript which does not contain the 3 UTR sequence, the VDAC protein is only weakly translated.
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Filep, Renee. "In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines." Thesis, This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-06102009-063451/.
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Valtink, Monika, Rita Gruschwitz, Richard H. W. Funk, and Katrin Engelmann. "Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136199.
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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastructure was monitored by scanning and transmission electron microscopy. Two clonal cell lines, HCEC-B4G12 and HCEC-H9C1, could be isolated and expanded, which differed morphologically: B4G12 cells were polygonal, strongly adherent and formed a strict monolayer, H9C1 cells were less adherent and formed floating spheres. The generation time of B4G12 cells was 62.26 ± 14.5 h and that of H9C1 cells 44.05 ± 5.05 h. Scanning electron microscopy revealed that B4G12 cells had a smooth cell surface, while H9C1 cells had numerous thin filopodia. Both cell lines expressed ZO-1 and occludin adequately, and little but well detectable amounts of connexin-43. Expression of HCEC-specific 9.3.E-antigen was found commensurately in both cell lines, while expression of Na,K-ATPase α1 was higher in H9C1 cells than in B4G12 cells. B4G12 cells expressed collagen IV abundantly and almost no collagen III, while H9C1 cells expressed both collagens at reasonable amounts. It is concluded that the clonal cell line B4G12 represents an ideal model of differentiated HCEC, while H9C1 may reflect features of developing or transitional HCEC<br>Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich
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Valtink, Monika, Rita Gruschwitz, Richard H. W. Funk, and Katrin Engelmann. "Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics." Karger, 2008. https://tud.qucosa.de/id/qucosa%3A27701.
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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastructure was monitored by scanning and transmission electron microscopy. Two clonal cell lines, HCEC-B4G12 and HCEC-H9C1, could be isolated and expanded, which differed morphologically: B4G12 cells were polygonal, strongly adherent and formed a strict monolayer, H9C1 cells were less adherent and formed floating spheres. The generation time of B4G12 cells was 62.26 ± 14.5 h and that of H9C1 cells 44.05 ± 5.05 h. Scanning electron microscopy revealed that B4G12 cells had a smooth cell surface, while H9C1 cells had numerous thin filopodia. Both cell lines expressed ZO-1 and occludin adequately, and little but well detectable amounts of connexin-43. Expression of HCEC-specific 9.3.E-antigen was found commensurately in both cell lines, while expression of Na,K-ATPase α1 was higher in H9C1 cells than in B4G12 cells. B4G12 cells expressed collagen IV abundantly and almost no collagen III, while H9C1 cells expressed both collagens at reasonable amounts. It is concluded that the clonal cell line B4G12 represents an ideal model of differentiated HCEC, while H9C1 may reflect features of developing or transitional HCEC.<br>Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
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Ka, Sojeong. "Gene Expression in the Brains of Two Lines of Chicken Divergently Selected for High and Low Body Weight." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl.[distributör], 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-108455.
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Mignolet, Alix. "Classification of polyphenolic compounds according to their differential effects on two breast cancer cell lines by FTIR spectroscopy." Doctoral thesis, Universite Libre de Bruxelles, 2017. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/246922.
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The development of reliable and cost-saving methods to select pre-clinically new potential drugs with unknown and original mechanisms of action for cancer therapy has become crucial. Previous investigations demonstrated that infrared spectra of cancer cells exposed to well-known anticancer drugs used in the clinics provide a global fingerprint of all the metabolic modifications induced. Nowadays several natural products have been recognized for their medicinal values. Polyphenolic compounds constitute one of the largest groups of plant metabolites and many studies have demonstrated their anticancer properties at multiple steps of carcinogenesis. Taking into account the large diversity of polyphenolic structures in nature and their numerous targets against carcinogenesis, the step of selection becomes essential as it is virtually impossible to classify them using traditional classification techniques. While FTIR spectroscopy appears to have a definite potential to sort anticancer drugs on the basis of the metabolic modifications they induced, the present challenge in this thesis is to evaluate the drug-induced spectral changes in cancer cells on a larger scale. The coupling of FTIR spectroscopy with a high throughput screening extension could become a useful method to generate drug classifications based on the “modes of action”. In a first step, the IC50 was evaluated for each polyphenol to normalize all spectral experiments that will be carried out on breast cancer cells. The first experiments revealed dispersive artifacts (wide variations of absorbance distribution and Mie scattering effects) contributing to spectral measurements. To minimize them, the best option was covering uniformly the surface of spectral measurement with cells (detailed in chapter III). Once the protocol refined, it was applied to the study of MDA-MB-231 breast cancer cells exposed for 24 hrs to 15 polyphenols. Through unsupervised and supervised statistical analyses, a distinction between polyphenol treatments could be well established. Complex effects of polyphenols on cancer cells were revealed, suggesting that mechanisms specific to each polyphenol were evidenced by the whole infrared spectrum. Clustering of polyphenol-induced spectral signatures by Hierarchical Cluster Analysis indicates that some of the polyphenols share similar effects on MDA-MB-231 (detailed in chapter IV). This experiment was then extended to another breast cancer cell line, MCF-7. It demonstrated a cell line dependency to polyphenolic treatments. Finally a subcellular investigation of treated MCF-7 breast cancer cells in their live state was done using Raman imaging. A distinction between nucleus and cytoplasm of treated cells brought supplementary information regarding the effect of polyphenols, leading to subcellular biological assumptions on polyphenol effects. These results paves the way for a new classification based on infrared spectral signatures that reflect the overall chemical modifications experienced by the cells when exposed to drugs.<br>Le développement de méthodes fiables et peu cher pour sélectionner de potentiels nouveaux médicaments présentant un mécanisme d’action original et inconnu avant toute étape clinique devient crucial. De précédentes études ont pu démontrer que les spectres infrarouges de cellules cancéreuses exposées à des agents anticancéreux connus et utilisé dans le monde clinique fournissent une empreinte globale de toutes les modifications métaboliques induites. La spectroscopie infrarouge est un outil innovant qui semble prometteur pour offrir un aperçu global des processus biologiques et physiologiques qui sont menés par un médicament dans des cellules cancéreuses. De nos jours, de nombreux produits naturels ont été reconnus pour leurs propriétés médicinales. Les polyphénols constituent l’un des plus vastes groupes de métabolites végétaux et de nombreuses études ont démontré leurs propriétés anticancéreuses à de multiples étapes de la carcinogénèse. En prenant en compte la très grande diversité de structures polyphénoliques existantes dans la nature et leurs nombreuses cibles anti-tumorales, l’étape de sélection est devenue essentielle comme il est virtuellement impossible de les classifier grâce à des techniques de classification traditionnelles telles que les études –omiques. Dès lors, le défi de cette thèse est d’évaluer les variations spectrales induites par un polyphénol dans des cellules cancéreuses à une plus grande échelle. Le couplage de la spectroscopie IRTF avec une extension de criblage de haut débit pourrait devenir une méthode utile pour générer des classifications de molécules sur base de leur « modes d’action ». Dans un premier temps, la concentration qui inhibe 50% de la croissance des cellules cancéreuses fut déterminée pour chaque polyphénol et chaque lignée de cellules cancéreuses. Le traitement des cellules à cette concentration permet une normalisation interne des expériences réalisées ultérieurement en spectroscopie infrarouge. Une fois le protocole établi, la lignée MDA-MB-231 fut exposée durant 24 heures à 15 polyphénols différents. Au moyen d’analyses statistiques multivariées supervisées et non supervisées, une distinction parmi les polyphénols a pu être établie et des effets complexes des polyphénols sur les cellules cancéreuses ont pu être révélés, suggérant des mécanismes d’action spécifiques à chaque polyphénol mis en évidence par spectroscopie infrarouge. Finalement, une étude subcellulaire sur cellules vivantes fut réalisée par imagerie Raman sur une seconde lignée de cellules cancéreuses mammaires appelées MCF-7. Cela permis de compléter en partie l’information macroscopique offerte par la spectroscopie infrarouge par une information microscopique sur l’effet de certains polyphénols. Cette thèse a ouvert la voie pour de nouvelles techniques de classification d’agents anticancéreux basées sur la spectroscopie infrarouge, technique sensible à l’ensemble des modifications chimiques subies par des cellules.<br>Option Chimie du Doctorat en Sciences<br>info:eu-repo/semantics/nonPublished
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Cui, Yuhai. "A structural and transcriptional comparative analysis of the S locus regions in two self-incompatible Brassica napus lines." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ43253.pdf.
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Gregory, Taylor, Devin Josey, Alexa Bancroft, et al. "RNA-Seq to analyze two related rat Growth Hormone-producing somatotroph cell lines for tissue-specific transcript expression." Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/asrf/2019/schedule/154.
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Growth Hormone (GH), also known as somatotropin, is a protein hormone secreted from the anterior pituitary gland. GH action results in longitudinal bone growth in children and adolescents and continues later to control a variety of metabolic reactions in adults. DNA polymorphisms leading to a decrease in GH expression result in persons of short stature, whereas those leading to overexpression of GH produce either acromegaly or gigantism. In order to diagnose and treat patients with altered GH production, understanding the control of GH expression is thus clinically relevant. In order to understand how transcription of GH is determined by selective regulatory modulators, two rat somatotroph cell lines were studied that differed in their relative differentiation: MtT/S cells are considered as fully differentiated somatotrophs that express GH exclusively; MtT/Se cells are related somatotrophs with GH expression that is responsive to estrogen treatment. These cell lines were obtained from the Riken Cell Bank (Japan) and then grown and cultured in accordance with established protocols. After harvesting of cells from each line, total RNA was extracted using a rapid affinity method (Qiagen). After validation of the RNA integrity index of each RNA isolate (Affymetrix), they were sent to an off-site laboratory (NovoGene) for RNA-Seq analysis. Samples were examined in triplicate for comparison using standard procedures for adapter ligation, library construction, amplification and sequencing. In our previous work with single gene quantitative RT-PCR, we measured expression of 9 separate targets, primarily transcriptional controllers, in these cell lines. Now, the use of RNA-Seq quantifies the levels of all mRNAs in each sample without the need for specific primers or probes. Therefore, it is possible to find nearly all of the mRNAs that demonstrate changing abundance without requiring prior evidence of their role in the terminal differentiation of the MtT/S from MtT/Se cells. After extensive QC for validation, initial analysis of the data shows more than 36 million validated sequenced reads from each replicate sample with >96.39% mapping to the reference rat genome sequence. Basic analysis shows that 13,012 expressed genes were 87% similar, with 1,175 (9.0%) unique to MtT/Se cells and 484 (3.7%) genes selectively present in MtT/S cells. Of these, 329 genes were upregulated in expression while only 57 were reduced. For comparison with our previous study, we are now confirming differences in GH transcripts and the transcription factors/regulators previously measured, particularly the GH regulator Zn16, a protein with 16 zinc fingers known to bind to the GH promoter DNA. Further analysis of the RNA-Seq profile is focused on the unique and unidentified changes in expression that differentiate these cell lines. Analysis of the differentiation of MtT/S and MtT/Se cells will further understanding of GH regulation in the body.
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Gilbert, Elizabeth R. "Dietary and Developmental Regulation of Nutrient Transporter Gene Expression in the Small Intestine of Two Lines of Broilers." Diss., Virginia Tech, 2008. http://hdl.handle.net/10919/28795.
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To better understand the digestive and absorptive capacities of the chick intestine so that we may feed diets that better meet the nutritional needs of the chick, it is important to understand how expression of nutrient transporter genes changes in response to various factors. A series of feeding trials were conducted to evaluate the dietary and developmental regulation of nutrient transporter mRNA abundance in the small intestine of two lines of broilers selected on corn-based (Line A) or wheat-based (Line B) diets. Abundance of mRNA was quantified in all experiments using real time PCR and the absolute quantification method. The objective of the first study was to investigate intestinal nutrient transporter and enzyme mRNA in Line A and B broilers at embryo day 18 and 20, day of hatch, and d 1, 3, 7, and 14 posthatch. Genes evaluated included the peptide transporter, PepT1, 10 AA transporters (rBAT, bo,+AT, ATBo,+, CAT1, CAT2, LAT1, y+LAT1, y+LAT2, BoAT and EAAT3), four sugar transporters (SGLT1, SGLT5, GLUT5, and GLUT2), and a digestive enzyme, APN. For PepT1, Line B had greater quantities of mRNA compared with Line A (P = 0.001), suggesting a greater capacity for absorption of AA as peptides. Levels of PepT1 mRNA were greatest in the duodenum (P < 0.05), whereas the abundances of SGLT1, GLUT5 and GLUT2 mRNA were greatest in the jejunum (P < 0.05). Abundances of EAAT3, bo,+AT, rBAT, BoAT, LAT1, CAT2, SGLT5 and APN mRNA were greatest in the ileum (P < 0.05). Quantities of PepT1, EAAT3, BoAT, SGLT1, GLUT5, and GLUT2 mRNA increased linearly (P < 0.01), while CAT1, CAT2, y+LAT1, and LAT1 mRNA decreased linearly (P < 0.05) with age. The objective of the second study was to evaluate the effect of dietary protein quality on intestinal peptide, AA, and glucose transporter, and digestive enzyme mRNA abundance in Line A and B broilers. At day of hatch (doh), chicks from both lines were randomly assigned to corn-based diets containing 24% crude protein (CP) with either soybean meal (SBM) or corn gluten meal (CGM) as the supplemental protein source, ad libitum. Groups of chicks from both lines were also assigned to the SBM diet at a quantity restricted to that consumed by the CGM group (SBM-RT). Abundance of PepT1, EAAT3, and GLUT2 mRNA was greater in Line B (P < 0.03), while APN and SGLT1 were greater in Line A (P < 0.04). When feed intake was equal (CGM vs restricted SBM), a greater abundance of PepT1 and bo,+AT mRNA was associated with the higher quality SBM (P < 0.04), while a greater abundance of EAAT3 and GLUT2 mRNA was associated with the lower quality CGM (P < 0.01). When feed intake was restricted (SBM vs SBM-RT), a greater abundance of PepT1 mRNA was associated with the restricted intake (P < 0.04). The objective of the third study was to determine the effect of dietary protein composition on mRNA abundance of peptide and AA transporters, and a digestive enzyme. From day 8 to day 15 posthatch, Line A and B broilers were fed equal amounts of 1 of 3 diets (24% CP). Dietary protein sources included whey protein concentrate (whey), a partial whey hydrolysate (hydro), or a mixture of free amino acids (AA) similar to the composition of whey. Intestine was collected at days 8, 9, 11, 13, and 15. Expression of all genes except LAT1 was greater (P < 0.05) in Line B compared with A. Abundance of PepT1, EAAT3, y+LAT2, CAT1, bo,+AT, and APN mRNA varied little across diets in Line A but for CAT1 mRNA was greatest (P = 0.005) in Line A birds that consumed the AA diet. Expression of these genes was greatest (P < 0.006) in Line B birds consuming the hydro diet. A greater (P < 0.05) age response of bo,+AT, EAAT3, CAT1, and APN mRNA was observed in birds consuming the hydro or AA diets relative to the whey diet. Results from these studies collectively demonstrate that nutrient transporter gene expression is responsive to a variety of factors, including developmental stage, dietary manipulation, and genetic selection. Information from these studies can be used to improve dietary formulation so that nutrient utilization is enhanced, resulting in improved growth of the broiler.<br>Ph. D.
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Smith, Kelsey. "Identifying Frogeye Leaf Spot Resistance in Two Elite Soybean Populations and Analysis of Agronomic Traits in Resistant Lines." OpenSIUC, 2021. https://opensiuc.lib.siu.edu/theses/2843.
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Soybeans (Glycine max L.) are an important crop globally for its food, feed, and oilpurposes. It is impacted by many diseases, including Cercospora sojina, the causal agent of Frogeye Leaf Spot (FLS). Chemical and cultural controls to this fungal pathogen are insufficient, so genetic resistance must be acquired for adequate control. To this end, two recombinant inbred populations were screened in a greenhouse setting for their relative resistance to FLS, and their genomes were analyzed for contributing quantitative trait loci (QTL). In the Essex ́ Forrest population, one QTL was discovered on chromosome 13, and in the Forrest ́ Williams 82 population, two QTL were identified on chromosomes 6 and 11, respectively. These populations were then also screened in a field setting for agronomic traits. These traits were analyzed to detect one superior line for both FLS resistance and advanced agronomic traits, F ́W 125. This line should be used in future breeding projects to increase FLS resistance and reduce linkage drag for other desired characteristics.
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Papageorgiou, Vassilios A. "Static two-dimensional calculation of the capacitance and impedance of open microstrip-like structures using variational methods." Thesis, This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-08182009-040513/.
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Diédhiou, Calliste Jérémie. "Mechanisms of salt tolerance sodium, chloride and potassium homeostasis in two rice lines with different tolerance to salinity stress /." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979864097.
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Tognatta, Reshmi [Verfasser]. "CNP-expressing progenitors display strikingly differential oligodendroglial or multipotent fates, as assessed by two Cre reporter lines / Reshmi Tognatta." Bonn : Universitäts- und Landesbibliothek Bonn, 2012. http://d-nb.info/1044867574/34.
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Yeoh, Sue-Ching. "Gene Expression Profiling Of Two Oral Squamous Cell Carcinoma Cell Lines Compared With Normal Oral Epithelium, Using Cdna Microarray." Thesis, Faculty of Dentistry, 2006. http://hdl.handle.net/2123/5040.
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Mahaye, Ntombikayise. "A central enrichment-based comparison of two alternative methods of generating transcription factor binding motifs from protein binding microarray data." Thesis, Rhodes University, 2013. http://hdl.handle.net/10962/d1003049.
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Characterising transcription factor binding sites (TFBS) is an important problem in bioinformatics, since predicting binding sites has many applications such as predicting gene regulation. ChIP-seq is a powerful in vivo method for generating genome-wide putative binding regions for transcription factors (TFs). CentriMo is an algorithm that measures central enrichment of a motif and has previously been used as motif enrichment analysis (MEA) tool. CentriMo uses the fact that ChIP-seq peak calling methods are likely to be biased towards the centre of the putative binding region, at least in cases where there is direct binding. CentriMo calculates a binomial p-value representing central enrichment, based on the central bias of the binding site with the highest likelihood ratio. In cases where binding is indirect or involves cofactors, a more complex distribution of preferred binding sites may occur but, in many cases, a low CentriMo p-value and low width of maximum enrichment (about 100bp) are strong evidence that the motif in question is the true binding motif. Several other MEA tools have been developed, but they do not consider motif central enrichment. The study investigates the claim made by Zhao and Stormo (2011) that they have identified a simpler method than that used to derive the UniPROBE motif database for creating motifs from protein binding microarray (PBM) data, which they call BEEML-PBM (Binding Energy Estimation by Maximum Likelihood-PBM). To accomplish this, CentriMo is employed on 13 motifs from both motif databases. The results indicate that there is no conclusive difference in the quality of motifs from the original PBM and BEEML-PBM approaches. CentriMo provides an understanding of the mechanisms by which TFs bind to DNA. Out of 13 TFs for which ChIP-seq data is used, BEEML-PBM reports five better motifs and twice it has not had any central enrichment when the best PBM motif does. PBM approach finds seven motifs with better central enrichment. On the other hand, across all variations, the number of examples where PBM is better is not high enough to conclude that it is overall the better approach. Some TFs bind directly to DNA, some indirect or in combination with other TFs. Some of the predicted mechanisms are supported by literature evidence. This study further revealed that the binding specificity of a TF is different in different cell types and development stages. A TF is up-regulated in a cell line where it performs its biological function. The discovery of cell line differences, which has not been done before in any CentriMo study, is interesting and provides reasons to study this further.
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Alvi, Mohammed M. "Lysosomal Targeting of a Gelatin-Doxorubicin Conjugate and Its Evaluation for Cell Damage in Two Model Breast Cancer Cell Lines." Thesis, University of the Sciences in Philadelphia, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=28154563.
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Doxorubicin (DOX) is one of the most potent anthracycline antibiotics used for treatment of multiple tumor types including breast cancer treatment. However, its efficacy is limited by fatal toxicities associated with DOX therapy causing damage to healthy tissues and organs. In this project, a high molecular weight gelatin-doxorubicin (GDOX) conjugate was synthesized and purified. Gelatin was linked to DOX in this conjugation via glygly spacer and an acid labile hydrazone bond. GDOX was characterized for molecular weight distribution of gelatin by size exclusion chromatography. Drug load on the conjugate was measured by UV-visible spectroscopy. DOX release from conjugate was measured at three different pH: 4.8, 6.5 and 7.4. A minimal DOX release of 9% ± 3 % was observed at systemic pH (7.4) and maximum release of 49 ± 1.8 % was observed at tumor microenvironment at pH 4.8. GDOX was then evaluated in an in vitro model consisting of two breast cancer cell lines: MCF7 cells and a triple negative breast cancer (TNBC) MDA-MB-231 cells for intracellular trafficking with emphasis on lysosomal targeting. Cell uptake and localization of an equivalent concentration of 10 µM DOX in GDOX was determined with fluorescence microscopy in these cells and fluorescence quantification was performed with Fiji software. Whole cell fluorescence showed localization of GDOX in both cell lines with higher conjugate accumulation in MDA-MB-231 cells. Lysosomal membrane permeabilization (LMP) was studied using a fluorescent labelled dextran. After 24 h, GDOX triggered 100% LMP in TNBC cells but surprisingly no LMP was observed in MCF7 cells. Finally, to evaluate GDOX induced DNA damage in these cells, TUNEL assay was performed which labels DNA strands breaks using a fluorescent tag. Images were captured on a fluorescent microscope and evaluated for percent apoptotic cell count. A similar extent of apoptosis was seen in both the cell lines and was comparable to free DOX. Overall, these results suggest that conjugate has potential of inducing toxicity to TNBC cells via lysosomal pathway which may induce apoptosis and thus has a potential for treatment of TNBC tumors. However, MCF7 cell studied results indicates a different pathway of toxicity in inducing apoptosis.
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Sumners, Lindsay Hart. "Immunological Response to Clostridium perfringens in Two Genetically Divergent Lines of Chickens as Influenced by Major Histocompatibility Complex (MHC) Genotype." Thesis, Virginia Tech, 2011. http://hdl.handle.net/10919/43370.
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Chickens genetically selected for low (LA) or high (HA) antibody response to sheep red blood
cells (SRBC) displayed a correlated change in major histocompatibility complex (MHC), so that
LA chickens were 96% B13 and HA chickens were 96% B21. During a clinical outbreak of
necrotic enteritis, B21B21 genotypes experienced significantly less mortality (6% vs. 13 %)
compared to B13B13 genotypes. A study was carried out to assess immunological differences
between LA and HA lines during exposure to Clostridium perfringens. In Experiment 1,
chickens were orally gavaged with a low (10^7 CFU/mL) or high (10^9 CFU/mL) dose of C.
perfringens. In Experiment 2, chickens were orally gavaged with live coccidia oocysts on
experiment d 1, followed by 107 CFU/mL C. perfringens on d 5. Unfortunately, establishment of
necrotic enteritis infection was unsuccessful in both experiments as evidenced by lack of
significant intestinal lesions, as well as no negative effect on bird performance. In an ex vivo
study, peripheral blood mononuclear cells (PBMCs) were isolated from each genetic line,
cultured, stimulated with LPS (4 h), and exposed to varying concentrations of C. perfringens α-
toxin (1, 10, 100, 1000 U/L) for 2 and 4 h. Evaluation of cellular proliferation, percent
cytotoxicity and immunological gene expression was carried out in a variety of experiments.
Genetic lines were found to be highly divergent in all analyses.<br>Master of Science
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Kalghatgi, Roshan Satish. "Reconstruction techniques for fixed 3-D lines and fixed 3-D points using the relative pose of one or two cameras." Thesis, Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/43590.
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In general, stereovision can be defined as a two part problem. The first is the correspondence problem. This involves determining the image point in each image of a set of images that correspond to the same physical point P. We will call this set of image points, N. The second problem is the reconstruction problem. Once a set of image points, N, that correspond to point P has been determined, N is then used to extract three dimensional information about point P.
This master's thesis presents three novel solutions to the reconstruction problem. Two of the techniques presented are for detecting the location of a 3-D point and one for detecting a line expressed in a three dimensional coordinate system. These techniques are tested and validated using a unique 3-D finger detection algorithm. The techniques presented are unique because of their simplicity and because they do not require the cameras to be placed in specific locations, orientations or have specific alignments. On the contrary, it will be shown that the techniques presented in this thesis allow the two cameras used to assume almost any relative pose provided that the object of interest is within their field of view.
The relative pose of the cameras at a given instant in time, along with basic equations from the perspective image model are used to form a system of equations that when solved, reveal the 3-D coordinates of a particular fixed point of interest or the three dimensional equation of a fixed line of interest. Finally, it will be shown that a single moving camera can successfully perform the same line and point detection accomplished by two cameras by altering the pose of the camera.
The results presented in this work are beneficial to any typical stereovision application because of the computational ease in comparison to other point and line reconstruction techniques. But more importantly, this work allows for a single moving camera to perceive three-dimensional position information, which effectively removes the two camera constraint for a stereo vision system. When used with other monocular cues such as texture or color, the work presented in this thesis could be as accurate as binocular stereo vision at interpreting three dimensional information. Thus, this work could potentially increase the three dimensional perception of a robot that normally uses one camera, such as an eye-in-hand robot or a snake like robot.
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Heimann, Felix [Verfasser], and Peter [Akademischer Betreuer] Bastian. "An Unfitted Higher-Order Discontinuous Galerkin Method for Incompressible Two-Phase Flow with Moving Contact Lines / Felix Heimann ; Betreuer: Peter Bastian." Heidelberg : Universitätsbibliothek Heidelberg, 2013. http://d-nb.info/117738101X/34.
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