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Journal articles on the topic 'Cox1 mtDNA'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Jia, Yaning, Weixia Li, Yanlin Li, et al. "The Levels of Polycyclic Aromatic Hydrocarbons and Their Derivatives in Plasma and Their Effect on Mitochondrial DNA Methylation in the Oilfield Workers." Toxics 11, no. 5 (2023): 466. http://dx.doi.org/10.3390/toxics11050466.

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This study focuses on the components and levels of polycyclic aromatic hydrocarbons (PAHs) and their derivatives (MPAHs and OPAHs) in plasma samples from 19 oil workers, pre- and post-workshift, and their exposure–response relationship with mitochondrial DNA (mtDNA) methylation. PAH, MPAH, OPAH, and platelet mtDNA methylation levels were determined using a gas chromatograph mass spectrometer (GC-MS) and a pyrosequencing protocol, respectively. The total plasma concentrations of PAHs in mean value were, respectively, 31.4 ng/mL and 48.6 ng/mL in pre- and post-workshift, and Phe was the most abu

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Nisztuk-Pacek, Sylwia, Brygida Ślaska, Ludmiła Grzybowska-Szatkowska, and Marek Babicz. "Paternal Leakage of Mitochondrial DNA in the Raccoon Dog (Nyctereutes Procyonoides Gray 1834)." Annals of Animal Science 19, no. 1 (2019): 61–69. http://dx.doi.org/10.2478/aoas-2018-0049.

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AbstractThe aim of the study was to describe the mechanism of mitochondrial DNA inheritance in a group of farmed raccoon dogs. The study involved 354 individuals. Whole peripheral blood was the research material. DNA was isolated and PCR was performed for two fragments of mitochondrial genes: COX1 (cytochrome oxidase subunit 1 gene) and COX2 (cytochrome oxidase subunit 2 gene). The PCR products were sequenced and subjected to bioinformatics analyses. Three mitochondrial haplotypes were identified in the COX1 gene fragment and two in the COX2 gene fragment. The analysis of mtDNA inheritance in

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Knoll, Aleš, Lucie Langová, Antonín Přidal, and Tomáš Urban. "Haplotype Diversity in mtDNA of Honeybee in the Czech Republic Confirms Complete Replacement of Autochthonous Population with the C Lineage." Insects 15, no. 7 (2024): 495. http://dx.doi.org/10.3390/insects15070495.

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The study aimed to analyze the genetic diversity in the Czech population of Apis mellifera using mitochondrial DNA markers, tRNAleu-cox2 intergenic region and cox1 gene. A total of 308 samples of bees were collected from the entire Czech Republic (from colonies and flowers in 13 different regions). Following sequencing, several polymorphisms and haplotypes were identified. Analysis of tRNAleu-cox2 sequences revealed three DraI haplotypes (C, A1, and A4). The tRNAleu-cox2 region yielded 10 C lineage haplotypes, one of which is a newly described variant. Three A lineage haplotypes were identifie

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Wei, T., X. Zhang, J. Cui, L. N. Liu, P. Jiang, and Z. Q. Wang. "Levels of sparganum infections and phylogenetic analysis of the tapeworm Spirometra erinaceieuropaei sparganum in wild frogs from Henan Province in central China." Journal of Helminthology 89, no. 4 (2014): 433–38. http://dx.doi.org/10.1017/s0022149x14000248.

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AbstractSparganosis is a serious food-borne parasitic zoonosis caused by infection with Spirometra spargana. The prevalence of sparganum infection in wild frogs (Rana nigromaculata, R. limmochari, R. temporaria and Bufo gargarizans) was investigated in Henan Province of central China during 2008–2012. Of 3482 caught wild frogs, 565 (16.23%) were found to be infected with plerocercoids (spargana) of the genus Spirometra. Spargana were found in 14.85% (320/2155) of R. nigromaculata, 20.82% (233/1119) of R. limmochari and 10.91% (12/110) of R. temporaria frogs. However, no sparganum was found in

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Tkaczyk-Wlizło, Angelika, Krzysztof Kowal, Kaja Ziółkowska, et al. "Mitochondrial DNA alternations in formalin-fixed paraffin-embedded tissue collected from dogs with malignant mammary gland tumours." Animal Science Papers and Reports 43, no. 2 (2025): 215–24. https://doi.org/10.2478/aspr-2025-0015.

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Abstract Formalin-fixed paraffin embedded (FFPE) tissue is the most common histopathological technique for tumour assessment in the veterinary pathology. Due to easy availability of FFPE blocks, the aim of this retrospective study was to identify molecular defects caused by mutations in mitochondrial DNA (mtDNA) in cases of canine malignant mammary gland tumours (MGTs). DNA was isolated from 88 FFPE samples, then quantity and quality analyses were performed. Amplification was conducted on 10 selected fragments of 8 mitochondrial genes (COX1, COX3, ND1, ND2, ND4, ND4L, ND5 and ATP6), and finall

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Liu, G. H., B. Li, J. Y. Li, et al. "Genetic variation among Clonorchis sinensis isolates from different geographic regions in China revealed by sequence analyses of four mitochondrial genes." Journal of Helminthology 86, no. 4 (2011): 479–84. http://dx.doi.org/10.1017/s0022149x11000757.

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AbstractThe present study examined sequence variation in four mitochondrial (mt) genes, namely cytochrome c oxidase subunits 1 (cox1) and 2 (cox2), and NADH dehydrogenase subunits 1 and 2 (nad1 and nad2) among Clonorchis sinensis isolates from different endemic regions in China, and their phylogenetic relationships with other zoonotic trematodes were reconstructed. A portion of the cox1 and cox2 genes (pcox1 and pcox2), and nad1 and nad2 genes (pnad1 and pnad2) were amplified separately from individual liver flukes by polymerase chain reaction (PCR) and the amplicons were subjected to sequenci

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Kowal, Krzysztof, Angelika Tkaczyk, Tomasz Ząbek, Mariusz Pierzchała, and Brygida Ślaska. "Comparative Analysis of CpG Sites and Islands Distributed in Mitochondrial DNA of Model Organisms." Animals 10, no. 4 (2020): 665. http://dx.doi.org/10.3390/ani10040665.

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The information about mtDNA methylation is still limited, thus epigenetic modification remains unclear. The lack of comprehensive information on the comparative epigenomics of mtDNA prompts comprehensive investigations of the epigenomic modification of mtDNA in different species. This is the first study in which the theoretical CpG localization in the mtDNA reference sequences from various species (12) was compared. The aim of the study was to determine the localization of CpG sites and islands in mtDNA of model organisms and to compare their distribution. The results are suitable for further

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Abou-Shaara, H. F. "A Scientific Note on the Evolutionary Relationships Between Honey Bees and Their Enemies." Cercetari Agronomice in Moldova 51, no. 1 (2018): 101–7. http://dx.doi.org/10.2478/cerce-2018-0009.

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Abstract Honey bees are used in intensive way in agriculture due to their vital role in pollination of crops. Moreover, there are many valuable products from the bee colonies. Unfortunately, there are many enemies to honey bees. These enemies belong to various taxonomic ranks, including birds, insects and mites. Serious damages can be caused to honey bee colonies by these enemies. The sophisticated evolutionary relationships between honey bees and their enemies are not well investigated. In this study, phylogenetic trees between honey bees and their enemies were constructed based on the mtDNA

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Simanjuntak, Juniarto Gautama, Windra Priawandiputra, Rika Raffiudin, et al. "Apis cerana Fabricius, 1793 in Sumatra: Haplotype Variations of Mitochondrial DNA and the Molecular Relationship with the Asian Honey Bees (Hymenoptera: Apidae)." HAYATI Journal of Biosciences 31, no. 4 (2024): 768–80. http://dx.doi.org/10.4308/hjb.31.4.768-780.

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Honey bee Apis cerana is widely distributed in Asia and the Indonesian archipelago, including Sumatra. We studied the molecular variations of A. cerana using cytochrome c oxidase subunits 1 and 2 genes (cox1 and cox2) and the cox1/cox2 intergenic spacers (igs) in several altitudes in the six provinces of Sumatra. We explored the haplotype distributions of those three mtDNA markers for A. cerana in the low-, mid-, and highlands of Sumatra. We also analyzed their relationship with A. cerana in Sundaland and Asia using those markers. Our study revealed 12 new haplotypes of A. cerana cox1 in Sumat

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Shumenko, Polina Gennadievna, and Yuliya Viktorovna Tatonova. "Using molecular markers to study epidemiologically important trematode representatives, Clonorchis sinensis and Metagonimus suifunensis, in the Russian Far East." Sanitarnyj vrač (Sanitary Doctor), no. 8 (July 6, 2021): 65–68. http://dx.doi.org/10.33920/med-08-2108-09.

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Despite the epidemiological significance of the trematodes Clonorchis sinensis and Metagonimus suifunensis, these species are insufficiently studied in the Russian Far East. In this work, we compare various nuclear and mitochondrial markers used for population genetic studies, based on previously published articles, as well as new data on the nad1 mtDNA gene obtained for M. suifunensis. Even such conservative markers as the ITS1–5.8S-ITS2 rDNA region and the cox1 mtDNA gene are suitable for the analysis of genetic variability in the C. sinensis population, while more variable mtDNA genes must

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Pedersen, H. S., P. Løvendahl, K. Larsen, L. B. Madsen, and H. Callesen. "181 mtDNA COPY NUMBER IN OOCYTES OF DIFFERENT SIZES FROM INDIVIDUAL PRE- AND POST-PUBERTAL PIGS." Reproduction, Fertility and Development 27, no. 1 (2015): 181. http://dx.doi.org/10.1071/rdv27n1ab181.

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Oocyte competence has been related to mtDNA copy number, but a large variation in mtDNA copy number between oocytes has been observed, caused by, e.g. oocyte donor and oocyte size (Sato et al. 2014 PLOS ONE 9, e94488; Cotterill et al. 2013 Mol. Hum. Reprod. 19, 444–450; El Shourbagy et al. 2006 Reproduction 131, 233–245). However, the correlation between size and mtDNA copy number in single oocytes has not been determined. This study describes the relation between oocytes of defined diameters from individual pre- and postpubertal pigs and mtDNA copy number. Cumulus-oocyte complexes were aspira

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Murata, Rie, Jun Suzuki, Ayako Hyuga, Takayuki Shinkai, and Kenji Sadamasu. "Molecular identification and characterization of Sarcocystis spp. in horsemeat and beef marketed in Japan." Parasite 25 (2018): 27. http://dx.doi.org/10.1051/parasite/2018026.

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Human Sarcocystis infections are known to be caused by the ingestion of raw or undercooked beef or pork containing mature sarcocysts of Sarcocystis hominis or S. suihominis, respectively. In addition, several cases of parasitic food poisoning in Japan have recently been reported after consumption of raw horsemeat containing sarcocysts of S. fayeri. In this study, the presence of sarcocysts in 28 horsemeat and 121 beef samples collected in Tokyo was investigated. Sarcocysts of S. fayeri were found in 16 horsemeat samples. Sarcocysts of S. hominis were not detected in beef samples, while sarcocy

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Jo, Areum, Sangwoo Ham, Gum Hwa Lee, et al. "Efficient Mitochondrial Genome Editing by CRISPR/Cas9." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/305716.

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The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show that regular FLAG-Cas9 can localize to mitochondria to edit mitochondrial DNA with sgRNAs targeting specific loci of the mitochondrial genome. Expression of FLAG-Cas9 together with gRNA targeting Cox1 and

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Stribinskis, Vilius, Guo-Jian Gao, Steven R. Ellis, and Nancy C. Martin. "Rpm2, the Protein Subunit of Mitochondrial RNase P in Saccharomyces cerevisiae, Also Has a Role in the Translation of Mitochondrially Encoded Subunits of Cytochrome c Oxidase." Genetics 158, no. 2 (2001): 573–85. http://dx.doi.org/10.1093/genetics/158.2.573.

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Abstract RPM2 is a Saccharomyces cerevisiae nuclear gene that encodes the protein subunit of mitochondrial RNase P and has an unknown function essential for fermentative growth. Cells lacking mitochondrial RNase P cannot respire and accumulate lesions in their mitochondrial DNA. The effects of a new RPM2 allele, rpm2-100, reveal a novel function of RPM2 in mitochondrial biogenesis. Cells with rpm2-100 as their only source of Rpm2p have correctly processed mitochondrial tRNAs but are still respiratory deficient. Mitochondrial mRNA and rRNA levels are reduced in rpm2-100 cells compared to wild t

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15

Hòa, Lê Thanh, Nguyễn Thị Khuê, Nguyễn Thị Bích Nga, et al. "Genetic characterization of mitochondrial genome of the small intestinal fluke, Haplorchis taichui (Trematoda: Heterophyidae), Vietnamese sample." Vietnam Journal of Biotechnology 14, no. 2 (2016): 215–24. http://dx.doi.org/10.15625/1811-4989/14/2/9333.

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The small intestinal fluke, Haplorchis taichui Nishigori, 1924, belonging to genus Haplorchis (family Heterophyidae, class Trematoda, phylum Platyhelminthes), is a zoonotic pathogen causing disease in humans and animals. Complete mitochondrial genome (mtDNA) of H. taichui (strain HTAQT, collected from Quang Tri) was obtained and characterized for structural genomics providing valuable data for studies on epidemiology, species identification, diagnosis, classification, molecular phylogenetic relationships and prevention of the disease. The entire nucleotide mtDNA sequence of H. taichui (HTAQT)

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Gupta, Yash Munnalal, Kittisak Buddhachat, Surin Peyachoknagul, and Somjit Homchan. "Novel DNA Barcode Sequence Discovery from Transcriptome of Acheta domesticus: A Partial Mitochondrial DNA." Materials Science Forum 967 (August 2019): 59–64. http://dx.doi.org/10.4028/www.scientific.net/msf.967.59.

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The potential of mitochondrial DNA (mtDNA) genes are well-known for species identification and to establish a phylogenetic relationship. The De-novo transcriptome assembly of Acheta domesticus commonly known as house cricket, is provides important segments of DNA fragments from mitochondrial DNA due to higher abundance of its mRNA. When the reference sequence with gene annotation is absent for assembling and aligning desire gene sequences, like in the present case, the most similar sequence is obtained from online insect mitochondrial genome database to find mitochondrial DNA conserved domains

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Marande, William, Julius Lukeš, and Gertraud Burger. "Unique Mitochondrial Genome Structure in Diplonemids, the Sister Group of Kinetoplastids." Eukaryotic Cell 4, no. 6 (2005): 1137–46. http://dx.doi.org/10.1128/ec.4.6.1137-1146.2005.

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ABSTRACT Kinetoplastid flagellates are characterized by uniquely massed mitochondrial DNAs (mtDNAs), the kinetoplasts. Kinetoplastids of the trypanosomatid group possess two types of mtDNA molecules: maxicircles bearing protein and mitoribosomal genes and minicircles specifying guide RNAs, which mediate uridine insertion/deletion RNA editing. These circles are interlocked with one another to form dense networks. Whether these peculiar mtDNA features are restricted to kinetoplastids or prevail throughout Euglenozoa (euglenids, diplonemids, and kinetoplastids) is unknown. Here, we describe the m

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Xie, Yue, Yijun Chen, Lidan Wang, et al. "Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm Varestrongylus eleguneniensis (Strongylida: Protostrongylidae)." International Journal of Molecular Sciences 23, no. 21 (2022): 13597. http://dx.doi.org/10.3390/ijms232113597.

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The takin lungworm Varestrongylus eleguneniensis (Strongylida: Protostrongylidae) causes lethal bronchopneumonia and represents severe threats to captive and wild populations. However, until now there has been very limited information available concerning the molecular epidemiology and evolutionary biology of V. eleguneniensis. Mitochondrial genomes (mtDNAs) can provide resources for investigations in these areas and, therefore, can assist with the surveillance and control of this lungworm. Herein, the complete mtDNA of V. eleguneniensis was sequenced and characterized with Illumina pipeline a

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CHEN, JIA-JIA, HUI FENG, YUAN-CHAO WANG, and XIAO-BO ZHENG. "Phytopythium nanjingense sp. nov. (Pythiaceae, Peronosporales) from southern China based on morphological and molecular characters." Phytotaxa 403, no. 3 (2019): 239. http://dx.doi.org/10.11646/phytotaxa.403.3.8.

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A new species of Peronosporales: Phytopythium nanjingense, isolated from the lake water of Nanjing in Jiangsu Province of China is described and illustrated based on morphological characters and supported by molecular phylogenetic analyses using ITS rDNA and Cox1 and Cox2 mtDNA sequences. Phytopythium nanjingense is heterothallic and characterized by aquatic habitat, ellipsoid, mostly obpyriform to ovoid-obpyriform, internally or internally nested proliferating sporangia with conspicuous apical papillae, smooth oogonia, hypogynous antheridia, filamentous antheridial cells, and plerotic or near

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Stolbunova, V. V., and E. A. Borovikova. "Influence of the Rate of Changes in the COX1 Gene on Body Size and Sexual Selection in Carp Hybridization." Биология внутренних вод, no. 6 (November 1, 2023): 853–68. http://dx.doi.org/10.31857/s0320965223060323.

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The influence of mtDNA cytochrome c-oxidase I gene fragment variability on body length was studied in twelve species of cyprinids, which may have hybrids with Rutilus rutilus L. and Abramis brama L., and in reciprocal hybrids (RA, AR) and alloplasmatic backcrosses (ARR, RAA) of roach (R) and bream (A). It has been established that the rate of nucleotide substitutions in COX1 is negatively related not only to body size but also to fish life span, which differentiates them into two groups: group I – species with a high rate of COX1 changes and a relatively small body size and group II – species

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Fan, Xiao-Jun, Chang Zhang, Hui Ren, Jian-Hong Liu, and Yan-Xia Mi. "Molecular systematics analysis of Lymantria dispar based on 18S rRNA and cox1 mtDNA sequence data." Zoological Systematics 40, no. 2 (2015): 228–34. https://doi.org/10.11865/zs.201500208.

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Fan, Xiao-Jun, Zhang, Chang, Ren, Hui, Liu, Jian-Hong, Mi, Yan-Xia (2015): Molecular systematics analysis of Lymantria dispar based on 18S rRNA and cox1 mtDNA sequence data. Zoological Systematics 40 (2): 228-234, DOI: 10.11865/zs.201500208

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Nisztuk-Pacek, Sylwia. "Genetic variability of farmed fur animals of the Canidae family assessed by nuclear and mitochondrial DNA analysis." Medycyna Weterynaryjna 72, no. 8 (2016): 505–10. http://dx.doi.org/10.21521/mw.5544.

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The aim of the study was to assess the biodiversity of farmed fur animals from the Canidae family (common fox, polar fox, and raccoon dog) using nuclear and mitochondrial markers. The study involved 434 animals. The biological material included whole peripheral blood or skin tissue. The isolated genetic material was subjected to qualitative and quantitative analyses. Mitochondrial DNA (mtDNA) gene fragments (COX1, COX2, CYTB) and nuclear DNA (nDNA) gene fragments (MSTN1, MSTN2, MSTN3, IGF1, GHR) were amplified with the PCR (polymerase chain reaction) technique. The amplicons obtained were sequ

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Kowal, Krzysztof, Brygida Ślaska, Adam Bownik, et al. "Analysis of Mitochondrial Genome from Labrador (Canis lupus familiaris) with Mammary Gland Tumour Reveals Novel Mutations and Polymorphisms." Annals of Animal Science 19, no. 3 (2019): 619–32. http://dx.doi.org/10.2478/aoas-2019-0027.

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AbstractThe aim of the study was to find associations between the process of neoplastic transformation and mtDNA mutations/polymorphisms, i.e. factors with potential prognostic significance, and to determine their impact on the biochemical properties, as well as structural, and functional properties of proteins. Blood and neoplastic tissue samples were collected from a 9-year-old Labrador dog with a diagnosed malignant mammary tumour. Next-generation genome sequencing (NGS) of the entire mitochondrial genome was performed using Illumina technology, and bioinformatics analyses were carried out.

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Cruz-Barraza, José Antonio, Cristina Vega, and José Luis Carballo. "Taxonomy of family Plakinidae (Porifera: Homoscleromorpha) from eastern Pacific coral reefs, through morphology and cox1 and cob mtDNA data." Zoological Journal of the Linnean Society 171, no. 2 (2014): 254–76. https://doi.org/10.1111/zoj.12137.

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Cruz-Barraza, José Antonio, Vega, Cristina, Carballo, José Luis (2014): Taxonomy of family Plakinidae (Porifera: Homoscleromorpha) from eastern Pacific coral reefs, through morphology and cox1 and cob mtDNA data. Zoological Journal of the Linnean Society 171 (2): 254-276, DOI: 10.1111/zoj.12137, URL: http://dx.doi.org/10.1111/zoj.12137

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Tkaczyk-Wlizło, Angelika, Krzysztof Kowal, Anna Śmiech, and Brygida Ślaska. "Whole Mitochondrial Genome Sequencing Analysis of Canine Testicular Tumours." International Journal of Molecular Sciences 25, no. 18 (2024): 9944. http://dx.doi.org/10.3390/ijms25189944.

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Currently, the molecular background based on mitochondrial DNA (mtDNA) analysis of canine testicular tumours is underestimated. The available data mostly focus on histopathological evaluations, with a few reports of nuclear genome (nDNA) studies. Tumourigenesis represents a highly complex and diverse genetic disorder, which can also encompass defects in mtDNA. The aim of this study was to identify molecular changes in whole mitochondrial genome sequences obtained from dogs affected by testicular tumours. Samples of blood, tumour, and healthy tissue were collected from each animal, and mtDNA (u

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Watts, Talina, Oleh Khalimonchuk, Rachel Z. Wolf, Edward M. Turk, Georg Mohr, and Dennis R. Winge. "Mne1 Is a Novel Component of the Mitochondrial Splicing Apparatus Responsible for Processing of a COX1 Group I Intron in Yeast." Journal of Biological Chemistry 286, no. 12 (2011): 10137–46. http://dx.doi.org/10.1074/jbc.m110.205625.

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Saccharomyces cerevisiae cells lacking Mne1 are deficient in intron splicing in the gene encoding the Cox1 subunit of cytochrome oxidase but contain wild-type levels of the bc1 complex. Thus, Mne1 has no role in splicing of COB introns or expression of the COB gene. Northern experiments suggest that splicing of the COX1 aI5β intron is dependent on Mne1 in addition to the previously known Mrs1, Mss116, Pet54, and Suv3 factors. Processing of the aI5β intron is similarly impaired in mne1Δ and mrs1Δ cells and overexpression of Mrs1 partially restores the respiratory function of mne1Δ cells. Mrs1 i

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Krampis, Konstantinos, Brett M. Tyler, and Jeffrey L. Boore. "Extensive Variation in Nuclear Mitochondrial DNA Content Between the Genomes of Phytophthora sojae and Phytophthora ramorum." Molecular Plant-Microbe Interactions® 19, no. 12 (2006): 1329–36. http://dx.doi.org/10.1094/mpmi-19-1329.

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Fragments of mitochondrial DNA (mtDNA) transferred to the nuclear genome are called nuclear mitochondrial DNAs (NUMTs). We report here a comparison of NUMT content between genomes from two species of the same genus. Analysis of the genomes of Phytophthora sojae and P. ramorum revealed large differences in the NUMT content of the two genomes: 16.27 × 10−3 and 2.28 × 10−3% of each genome, respectively. Substantial differences also exist between the two species in the sizes of the NUMTs found in each genome, with ranges of 20 to 405 bp for P. sojae and 19 to 137 bp for P. ramorum. Furthermore, in

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Krivopalov, Anton, Pavel Vlasenko, Sergey Abramov, et al. "Distribution and Molecular Diversity of Paranoplocephala kalelai (Tenora, Haukisalmi & Henttonen, 1985) Tenora, Murai & Vaucher, 1986 in Voles (Rodentia: Myodes) in Eurasia." Diversity 14, no. 6 (2022): 472. http://dx.doi.org/10.3390/d14060472.

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Cestodes Paranoplocephala kalelai, which parasitizes in the small intestine of Myodes voles and is distributed in northern Fennoscandia, was found in six habitats in the Asian part of Russia and eastern Kazakhstan, which indicates a wider distribution of P. kalelai on the continent. Analysis of mtDNA showed that P. kalelai is characterized by significant molecular variability in Eurasia. This study complements the data on the distribution of P. kalelai and provides the first molecular data from the territory of Russia and Kazakhstan. The sequence variability of two mitochondrial genes cox1 and

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Ray, Rudra, John Hamre, Kang Le, et al. "Exploring Mitochondrial DNA Heteroplasmy in a Mouse Model of Sickle Cell Disease." Blood 142, Supplement 1 (2023): 3858. http://dx.doi.org/10.1182/blood-2023-189660.

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BACKGROUND: Mitochondria are important in the pathology of sickle cell disease (SCD). They are abnormally retained in sickle red blood cells (RBCs) and the likely source of cell-free mitochondrial DNA (mtDNA), an erythrocytic-DAMP that triggered formation of neutrophil extracellular traps (Tumburu et al, 2022). Acquired mutations in the mitochondrial genome, referred to mtDNA heteroplasmy, are emerging markers of aging and inflammation. Using whole genome sequence data from peripheral blood DNA, we previously established that patients with SCD not only have increased mtDNA heteroplasmy, but th

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Valentyne, Hayley, David M. Spratt, Mahdis Aghazadeh, Malcolm K. Jones, and Jan Šlapeta. "The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis." Parasitology 147, no. 6 (2020): 681–88. http://dx.doi.org/10.1017/s0031182020000232.

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AbstractThe native rat lungworm (Angiostrongylus mackerrasae) and the invasive rat lungworm (Angiostrongylus cantonensis) occur in eastern Australia. The species identity of A. mackerrasae remained unquestioned until relatively recently, when compilation of mtDNA data indicated that A. mackerrasae sensu Aghazadeh et al. (2015b) clusters within A. cantonensis based on their mitochondrial genomes (mtDNA). To re-evaluate the species identity of A. mackerrasae, we sought material that would be morphologically conspecific with A. mackerrasae. We combined morphological and molecular approaches to co

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De Elías-Escribano, Alejandra, Irene Serafín-Pérez, Patricio Artigas, et al. "Wide Variation of Aedes albopictus Genotypes First Introduced into Canary Islands Assessed by rDNA Internal Transcribed Spacer Region and mtDNA cox1 Sequencing and Cloning." Tropical Medicine and Infectious Disease 10, no. 2 (2025): 35. https://doi.org/10.3390/tropicalmed10020035.

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Aedes albopictus, one of the most rapidly spreading invasive mosquito species, has expanded from Asia to establish populations on every continent except Antarctica, showcasing exceptional adaptability, particularly in island environments. This study provides the first molecular characterization of Ae. albopictus in the Canary Islands, Spain. Genotyping was conducted using rDNA 5.8S-ITS2 and mtDNA cox1 sequencing, with haplotype analysis and phylogenetic network assessment. Among 49 sequences, 28 distinct 5.8S-ITS2 haplotypes were identified, with individual specimens containing 5 to 17 haploty

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SPIRIDONOV, SERGEI E., and DUNG THE ĐINH. "Heth longquani sp. n.—(Rhigonematomorpha: Hethidae)—obligate commensalistic nematode of camballoid millipede (Spirostreptida: Cambalopsidae) from Viet Nam." Zootaxa 5325, no. 2 (2023): 251–60. http://dx.doi.org/10.11646/zootaxa.5325.2.6.

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A new nematode species, Heth longquani sp. n. (Hethidae, Ransonematoidea, Rhigonematomorpha, Rhabditida) is described from the camballoid millipede, Chonecambala crassicauda from Thanh Hóa province, Viet Nam. The morphological features of this new species were studied with brightfield and scanning electron microscopies. Partial sequences of LSU rDNA and Cox1 mtDNA are provided. The phylogeny of the nematodes of the genus Heth Cobb, 1898 is discussed.

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Morais, Sirlei Antunes, Fábio de Almeida, Lincoln Suesdek, and Mauro Toledo Marrelli. "Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina." Revista do Instituto de Medicina Tropical de São Paulo 54, no. 6 (2012): 325–29. http://dx.doi.org/10.1590/s0036-46652012000600007.

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Culex quinquefasciatus is a vector of human pathogens, including filarial nematodes and several viruses. Although its epidemiological relevance is known to vary across geographical regions, an understanding of its population genetic structure is still incipient. In light of this, we evaluated the genetic diversity of Cx. quinquefasciatus and Cx. pipiens x Cx. quinquefasciatus hybrids collected from nine localities in Brazil and one site in Argentina. We used mitochondrial genes cox1 and nd4, along with the coxA and wsp genes of the maternally-inherited Wolbachia endosymbiont. The nd4 fragment

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Čisovská Bazsalovicsová, Eva, Alžbeta Radačovská, Antti Lavikainen, Roman Kuchta, and Ivica Králová-Hromadová. "Genetic interrelationships of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidea), the causative agent of sparganosis in Europe." Parasite 29 (2022): 8. http://dx.doi.org/10.1051/parasite/2022009.

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The geographic distribution of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidea), the causative agent of food/water-borne sparganosis, is restricted to Europe, where infected canids, felids, mustelids, suids, and reptiles have been documented from Poland, Ukraine, Belarus, Russia, Serbia, Estonia, Latvia, and Finland. The main objective of the current study was to map the molecular divergence of S. erinaceieuropaei from Finland using the complete sequences of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1 mtDNA). Seven cox1 haplotypes were determined in 15 tapeworms from E

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Chuang, Yao-Chung, Shang-Der Chen, Chung-Yao Hsu, Shu-Fang Chen, Nai-Ching Chen та Shuo-Bin Jou. "Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway". International Journal of Molecular Sciences 20, № 4 (2019): 998. http://dx.doi.org/10.3390/ijms20040998.

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Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is known to regulate mitochondrial biogenesis. Resveratrol is present in a variety of plants, including the skin of grapes, blueberries, raspberries, mulberries, and peanuts. It has been shown to offer protective effects against a number of cardiovascular and neurodegenerative diseases, stroke, and epilepsy. This study examined the neuroprotective effect of resveratrol on mitochondrial biogenesis in the hippocampus following experimental status epilepticus. Kainic acid was microinjected into left hippocampal CA3 in S

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Zamora-Muñoz, Carmen, Cesc Múrria, Núria Bonada, and Marcos González. "The Hydropsyche instabilis group (Trichoptera: Hydropsychidae) on the Iberian Peninsula: evolutionary relationships, new species, taxonomical controversies, and a key to larvae. Arthropod Systematics & Phylogeny 75 (1): 159-172 PDF 1.7 MB." Arthropod Systematics & Phylogeny 75, no. 1 (2017): 159–72. http://dx.doi.org/10.3897/asp.75.e31882.

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The Hydropsyche instabilis group is a diverse Palearctic group composed of more than 30 species, many of them local endemics. The exact number of species in the Mediterranean Basin remains ambiguous, mainly due to the high variability of the genitalia and the lack of indepth taxonomic studies in some areas. In this paper, we describe the adult male and the larva of a new species from the Iberian Peninsula (Hydropsyche solerorum sp.n.), and provide information about the ecological requirements of the larvae. We also discuss the identity of a species, provisionally designated as Hydropsyche cf.

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Zamora-Muñoz, Carmen, Cesc Múrria, Núria Bonada, and Marcos González. "The Hydropsyche instabilis group (Trichoptera: Hydropsychidae) on the Iberian Peninsula: evolutionary relationships, new species, taxonomical controversies, and a key to larvae. Arthropod Systematics & Phylogeny 75 (1): 159-172 PDF 1.7 MB." Arthropod Systematics & Phylogeny 75 (April 5, 2017): 159–72. https://doi.org/10.3897/asp.75.e31882.

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The Hydropsyche instabilis group is a diverse Palearctic group composed of more than 30 species, many of them local endemics. The exact number of species in the Mediterranean Basin remains ambiguous, mainly due to the high variability of the genitalia and the lack of indepth taxonomic studies in some areas. In this paper, we describe the adult male and the larva of a new species from the Iberian Peninsula (Hydropsyche solerorum sp.n.), and provide information about the ecological requirements of the larvae. We also discuss the identity of a species, provisionally designated as Hydropsyche cf.

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Pedersen, Hanne Skovsgaard, Peter Løvendahl, Knud Larsen, Lone Bruhn Madsen, and Henrik Callesen. "Porcine oocyte mtDNA copy number is high or low depending on the donor." Zygote 24, no. 4 (2015): 617–23. http://dx.doi.org/10.1017/s0967199415000611.

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SummaryOocyte capacity is relevant in understanding decreasing female fertility and in the use of assisted reproductive technologies in human and farm animals. Mitochondria are important to the development of a functionally good oocyte and the oocyte mtDNA copy number has been introduced as a useful parameter for prediction of oocyte competence. The aim of this study was to investigate: (i) if the oocyte donor has an influence on its oocyte's mtDNA copy number; and (ii) the relation between oocyte size and mtDNA copy number using pre- and postpubertal pig oocytes. Cumulus–oocyte complexes were

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Wang, C. R., J. F. Gao, Q. C. Chang, F. C. Zou, Q. Zhao, and X. Q. Zhu. "Sequence variability in four mitochondrial genes among Bunostomum trigonocephalum isolates from four provinces in China." Journal of Helminthology 87, no. 4 (2012): 416–21. http://dx.doi.org/10.1017/s0022149x12000570.

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AbstractThe present study examined sequence variability in four mitochondrial genes, namely cytochrome c oxidase subunit (cox1), cytochrome b (cytb) and NADH dehydrogenase subunits 1 and 5 (nad1 and nad5), among Bunostomum trigonocephalum isolates from four different geographic regions in China. Ten B. trigonocephalum samples were collected from each of the four provinces (Heilongjiang, Jilin, Shaanxi and Yunnan), China. A part of the cox1 (pcox1), cytb (pcytb), nad1 and nad5 genes (pnad1 and pnad5) were amplified separately from individual hookworms by polymerase chain reaction (PCR) and were

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Josef, Finsterer. "12S-rRNA and COX1 carriers require comprehensive individual and family investigations and follow-up." International Journal Of Biology And Medicine 1, no. 1 (2019): 01–02. https://doi.org/10.36811/ijbm.2019.110001.

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In a recent article, Ding et al. reported about the diagnostic-work up of non-syndromic hearing loss in a Han Chinese family revealing two different mtDNA mutations [1]. The two variants were made responsible for the phenotype but there are some concerns with regard to the reliability of the presented data. The main shortcoming of the study is that no heteroplasmy rates of the m.1494C>T and m.7444G>A variant were provided. Phenotypic expression of an mtDNA variant may not only depend on the type and location of a mutation, on the penetrance, haplotypes, and polymorphisms, but also on het

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Valizadeh, M., F. Tahvildar Biderouni, S. R. Shahrokhi, M. Ghanimatdan, and A. R. Nagahi. "Sequencing and phylogenetic analysis of mitochondrial cox1 and nad1 genes in Toxocara canis and Toxascaris leonina isolates from Iran." BULGARIAN JOURNAL OF VETERINARY MEDICINE 24, no. 2 (2021): 251–60. http://dx.doi.org/10.15547/bjvm.2274.

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Toxocara canis and Toxascaris leonina are the most important ascaridoid nematodes of the family Toxocaridae. The present study was aimed to characterisation and analysis of genetic variation within and among T. canis and T. leonina isolates obtained from Iran by sequencing partial mitochondrial cytochrome c oxidase subunit 1 (pcox1) and partial NADH dehydrogenase subunit 1 (pnad1) genes. A total number of 134 adult nematodes belonging to Toxocaridae family were collected from stray dogs in Alborz province, Iran during 2015 and 2016. Polymerase chain reaction (PCR) was performed and products we

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Izrailskaia, Anna V., Yulia V. Tatonova, and Yulia V. Belousova. "New Genus and Two New Species of Notocotylidae Lühe, 1909 (Digenea), from Russia: Morphomolecular Data." Journal of Zoological Systematics and Evolutionary Research 2024 (April 18, 2024): 1–12. http://dx.doi.org/10.1155/2024/5550780.

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The study of the trematodes of the family Notocotylidae Lühe, 1909, is continued using an integrated approach based on new data. A new genus, Pseudonotocotylus n. gen., has been identified, and two species new to science, Notocotylus multipapillus n. sp. and Pseudonotocotylus martynenkoi n. sp., have been discovered. The life cycle and morphology of developmental stages of the new species are described, and the molecular data for nuclear (28S) and mitochondrial markers (cox1 and nad1) are presented. Sequences of cox1 and nad1 mtDNA genes were obtained for five previously studied notocotylid sp

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de Almeida, Jaqueline Raquel, Diego Mauricio Riaño Pachón, Livia Maria Franceschini, et al. "Revealing the high variability on nonconserved core and mobile elements of Austropuccinia psidii and other rust mitochondrial genomes." PLOS ONE 16, no. 3 (2021): e0248054. http://dx.doi.org/10.1371/journal.pone.0248054.

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Mitochondrial genomes are highly conserved in many fungal groups, and they can help characterize the phylogenetic relationships and evolutionary biology of plant pathogenic fungi. Rust fungi are among the most devastating diseases for economically important crops around the world. Here, we report the complete sequence and annotation of the mitochondrial genome of Austropuccinia psidii (syn. Puccinia psidii), the causal agent of myrtle rust. We performed a phylogenomic analysis including the complete mitochondrial sequences from other rust fungi. The genome composed of 93.299 bp has 73 predicte

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Pantoja, Camila, Fabiano Paschoal, Jorge Luiz Silva Nunes, and Hudson Alves Pinto. "Tubulovesicula lindbergi (Layman, 1930) (Digenea: Hemiuridae) in the Southwestern Atlantic Ocean: A Morphological and Phylogenetic Study Based on Specimens Found in Nebris microps (Actinopterygii: Sciaenidae) off the Brazilian Coast." Taxonomy 4, no. 3 (2024): 447–63. http://dx.doi.org/10.3390/taxonomy4030022.

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This study presents the first record of T. lindbergi from the southwestern Atlantic Ocean, based on specimens collected from the smalleye croaker Nebris microps Cuvier (Sciaenidae), off the coast of Maranhão Island, State of Maranhão, Brazil. Our approach included a morphological analysis complemented by DNA sequencing (28S, ITS2 rDNA, and cox1 mtDNA). Our phylogenetic analysis revealed the affinity of T. lindbergi to its congener T. laticaudi Parukhin, 1969, a digenean parasite commonly found in hydrophiine snakes inhabiting the Pacific Ocean. The interspecific divergence between T. lindbergi

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Li, F., T. Hu, N. C. Duan, et al. "Sequence variation in two mitochondrial DNA regions and internal transcribed spacer among isolates of the nematode Oesophagostomum asperum originating from goats in Hunan Province, China." Journal of Helminthology 90, no. 1 (2014): 1–6. http://dx.doi.org/10.1017/s0022149x14000650.

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AbstractThe present study examined sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1), and internal transcribed spacer (ITS) of nuclear ribosomal DNA (rDNA) among Oesophagostomum asperum isolates from goats in Hunan Province, China. A portion of the cox1 (pcox1), nad1 (pnad1) genes and the ITS (ITS1+5.8S rDNA+ITS2) rDNA were amplified by polymerase chain reaction (PCR) separately from adult O. asperum individuals and the representative amplicons were subjected to sequencing from both directions. Th

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Park, Jina, Yukyung Kim, Eggy Triana Putri, and Joong-Ki Park. "Revisiting the taxonomy of Korean Ischnochiton species (Polyplacophora, Ischnochitonidae) based on a combined analysis of morphological and molecular data." Biodiversity Data Journal 12 (October 23, 2024): e134521. https://doi.org/10.3897/BDJ.12.e134521.

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Chiton species belonging to the genus <i>Ischnochiton</i> J. E. Gray, 1847 are commonly found in intertidal rocky shores worldwide, with the exception of the northern Atlantic and Arctic oceans. <i>Ischnochiton</i> species are characterised by imbricate girdle scales that are uniform in size, rounded, sculpted with striae or occasionally smooth. However, their species-level taxonomy is complicated due to the high variation in their shell microstructures. Despite more than a hundred species reported worldwide, taxonomic studies of this group remain relatively unexplored in Korean waters, with o

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Romanov, D. A., E. V. Shaikevich, and I. A. Zakharov. "Genetic Introgression in Populations of Two Related Species <i>Adalia frigida</i> (Schneider, 1792) and <i>Adalia bipunctata</i> (Linnaeus, 1758) (Coleoptera: Coccinellidae) in the Zone of Sympatry." Генетика 59, no. 4 (2023): 448–59. http://dx.doi.org/10.31857/s0016675823040100.

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The haplotype diversity of mtDNA of the circumpolar species of ladybirds Adalia frigida collected in Yakutsk and Salekhard was studied. Based on the analysis of the nucleotide sequences of the cox1 gene, 18 mitochondrial haplotypes were identified, of which 14 were new. Of the 18 haplotypes of A. frigida, two (H32 and H9) are the most common. In the Salekhard region, the range of A. frigida overlaps with that of another closely related species, A. bipunctata. The value of divergence in the cox1 gene between A. frigida and A. bipunctata reaches 4.1–4.3% and corresponds to the level of differenc

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FREITAG, HENDRIK, and JÁN KODADA. "Larvae of Ancyronyx Erichson, 1847 (Insecta: Coleoptera: Elmidae) from Sulawesi, using DNA sequences for the assignment of the larval stages." Zootaxa 4299, no. 1 (2017): 121. http://dx.doi.org/10.11646/zootaxa.4299.1.6.

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We describe the immature stages of six Ancyronyx (Spider Riffle Beetles) species from Sulawesi: A. henningi Freitag & Kodada, 2017; A. longiparamerus Freitag & Kodada, 2017; A. skalei Freitag & Kodada, 2017; A. schoedli Freitag & Kodada, 2017; A. tobada Freitag & Kodada, 2017 and A. toraja Freitag & Kodada, 2017. The larvae were assigned to adults by comparison of their Cox1 gene fragment (mtDNA) which was amplified, sequenced and aligned with the corresponding locus of identified adults. The larvae are illustrated with habitus photographs and SEM micrographs.

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Poorani, J., S. K. Jalali, and Rakshit Ojha. "Morphological characters and DNA barcodes to separate Oenopia sauzeti Mulsant and O. mimica Weise (Coleoptera: Coccinellidae), two externally similar lady beetles from the Indian subcontinent." ENTOMON 40, no. 4 (2015): 235–42. http://dx.doi.org/10.33307/entomon.v40i4.98.

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Oenopia sauzeti Mulsant and O. mimica Weise (Coleoptera: Coccinellidae) are externally very similar and commonly misidentified species of lady beetles distributed in the Indian subcontinent. Diagnostic characters including male genitalia are illustrated for these species to facilitate their identification. The cox1 mtDNA sequences of O. sauzeti and O. mimica (658 bp) had only 89% similarity upon pair-wise alignment, which distinguished them with 75 nucleotide differences, thus confirming that these are distinct species. DNA barcodes with accession numbers AGIMP042-15 for O. sauzeti and AGIMP04

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Levy, A. A., C. P. André, and V. Walbot. "Analysis of a 120-kilobase mitochondrial chromosome in maize." Genetics 128, no. 2 (1991): 417–24. http://dx.doi.org/10.1093/genetics/128.2.417.

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Abstract The organization of the mitochondrial genome in plants is not well understood. In maize mitochondrial DNA (mtDNA) several subgenomic circular molecules as well as an abundant fraction of linear molecules have been seen by electron microscopy. It has been hypothesized that the circular molecules are the genetic entities of the mitochondrial genome while the linear molecules correspond to randomly sheared mtDNA. A model has been proposed that explains the mechanism of generation of subgenomic circles (of a predictable size) by homologous recombination between pairs of large direct repea

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