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Journal articles on the topic 'CPRI'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

Smidt, Hauke, Maarten van Leest, John van der Oost, and Willem M. de Vos. "Transcriptional Regulation of the cpr Gene Cluster inortho-Chlorophenol-Respiring Desulfitobacterium dehalogenans." Journal of Bacteriology 182, no. 20 (October 15, 2000): 5683–91. http://dx.doi.org/10.1128/jb.182.20.5683-5691.2000.

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ABSTRACT To characterize the expression and possible regulation of reductive dehalogenation in halorespiring bacteria, a 11.5-kb genomic fragment containing the o-chlorophenol reductive dehalogenase-encoding cprBA genes of the gram-positive bacterium Desulfitobacterium dehalogenans was subjected to detailed molecular characterization. Sequence analysis revealed the presence of eight designated genes with the ordercprTKZEBACD and with the same polarity except forcprT. The deduced cprC and cprKgene products belong to the NirI/NosR and CRP-FNR families of transcription regulatory proteins, respectively. CprD and CprE are predicted to be molecular chaperones of the GroEL type, whereascprT may encode a homologue of the trigger factor folding catalysts. Northern blot analysis, reverse transcriptase PCR, and primer extension analysis were used to elucidate the transcriptional organization and regulation of the cpr gene cluster. Results indicated halorespiration-specific transcriptional induction of the monocistronic cprT gene and the biscistroniccprBA and cprZE genes. Occasional read-through at cprC gives rise to a tetracistronic cprBACDtranscript. Transcription of cprBA was induced 15-fold upon addition of the o-chlorophenolic substrate 3-chloro-4-hydroxyphenylacetic acid within 30 min with concomitant induction of dehalogenation activity. Putative regulatory protein binding motifs that to some extent resemble the FNR box were identified in the cprT-cprK and cprK-cprZ intergenic regions and the promoter at cprB, suggesting a role for FNR-like CprK in the control of expression of thecprTKZEBACD genes.

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2

Villemur, Richard, Maude Saucier, Annie Gauthier, and Réjean Beaudet. "Occurrence of several genes encoding putative reductive dehalogenases inDesulfitobacterium hafniense/frappieriandDehalococcoides ethenogenes." Canadian Journal of Microbiology 48, no. 8 (August 1, 2002): 697–706. http://dx.doi.org/10.1139/w02-057.

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Desulfitobacterium frappieri PCP-1 has the capacity to dehalogenate several halogenated aromatic compounds by reductive dehalogenation, however, the genes encoding the enzymes involved in such processes have not yet been identified. Using a degenerate oligonucleotide corresponding to a conserved sequence of CprA/PceA reductive dehalogenases, a cprA-like gene fragment was amplified by PCR from this bacterial strain. A Delfitobacterium frappieri PCP-1 cosmid library was screened with the PCR product, allowing the cloning and sequencing of a 1.9-kb fragment. This fragment contains a nucleic acid sequence identical to one genomic contig of Desulfitobacterium hafniense, a bacterium closely related to Delfitobacterium frappieri that is also involved in reductive dehalogenation. Other genes related to the Desulfitobacterium dehalogenans cpr locus were identified in this contig. Interestingly, the gene arrangement shows the presence of two copies of cprA-, cprB-, cprC-, cprD-, cprK-, and cprT-related genes, suggesting that gene duplication occurred within this chromosomic region. The screening of Delfitobacterium hafniense genomic contigs with a CprA-deduced amino acid sequence revealed two other cprA-like genes. Microbial genomes available in gene databases were also analyzed for sequences related to CprA/PceA. Two open reading frames encoding other putative reductive dehalogenases in Delfitobacterium hafniense contigs were detected, along with 17 in the Dehalococcoides ethenogenes genome, a bacterium involved in the reductive dehalogenation of tetrachloroethene to ethene. The fact that several gene encoding putative reductive dehalogenases exist in Delfitobacterium hafniense, probably in other members of the genus Desulfitobacterium, and in Dehalococcoides ethenogenes suggests that these bacteria use distinct but related enzymes to achieve the dehalogenation of several chlorinated compounds.Key words: Desulfitobacterium, reductive dehalogenases, halorespiration, chlorinated compounds, gene family.

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3

Liu, Fushui, Jianyu You, Qi Li, Ting Fang, Mei Chen, Nana Tang, and Xiaojun Yan. "Acupuncture for Chronic Pain-Related Insomnia: A Systematic Review and Meta-Analysis." Evidence-Based Complementary and Alternative Medicine 2019 (June 24, 2019): 1–10. http://dx.doi.org/10.1155/2019/5381028.

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Objectives. Acupuncture has been widely used to relieve chronic pain-related insomnia (CPRI). However, the efficacy of acupuncture for CPRI is uncertain. The purpose of this study was to evaluate the efficacy of acupuncture for CPRI. Methods. Seven electronic databases were searched from inception to December 2018. Randomized controlled trials (RCTs) were included if acupuncture was compared to sham acupuncture or conventional drug therapies for treating CPRI. Two reviewers screened each study and extracted data independently. Statistical analyses were conducted by RevMan 5.3 software. Results. A total of nine studies involving 944 patients were enrolled. The pooled analysis indicated that acupuncture treatment was significantly better than control group in improving effective rate (OR = 8.09, 95%CI = [4.75, 13.79], P < 0.00001) and cure rate (OR = 3.17, 95%CI = [2.35, 4.29], P < 0.00001), but subgroup analysis showed that there was no statistically significant difference between acupuncture and sham acupuncture in improving cure rate (OR =10.36, 95% CI [0.53, 201.45], P=0.12) based on one included study. In addition, meta-analysis demonstrated that acupuncture group was superior to control group in debasing PSQI score (MD = -2.65, 95%CI = [-4.00, -1.30], P = 0.0001) and VAS score (MD = -1.44, 95%CI = [-1.58, -1.29], P < 0.00001). And there was no significant difference in adverse events (OR =1.73, 95%CI = [0.92, 3.25], P =0.09) between the two groups. Conclusions. Acupuncture therapy is an effective and safe treatment for CPRI, and this treatment can be recommended for the management of patients with CPRI. Due to the low quality and small sample size of the included studies, more rigorously designed RCTs with high quality and large sample size are recommended in future.

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4

Poon, Henry C., Roland Carson, Frank Peter, Benjamin Goldberg, and Heinz L. Haust. "The lead program at CPRI." Clinical Biochemistry 22, no. 3 (June 1989): 213–19. http://dx.doi.org/10.1016/s0009-9120(89)80079-7.

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5

Gomes, Nathan J., Philippe Chanclou, Peter Turnbull, Anthony Magee, and Volker Jungnickel. "Fronthaul evolution: From CPRI to Ethernet." Optical Fiber Technology 26 (December 2015): 50–58. http://dx.doi.org/10.1016/j.yofte.2015.07.009.

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6

Tonini, Federico, Bahare Khorsandi, Steinar Bjornstad, Raimena Veisllari, and Carla Raffaelli. "C-RAN Traffic Aggregation on Latency-Controlled Ethernet Links." Applied Sciences 8, no. 11 (November 18, 2018): 2279. http://dx.doi.org/10.3390/app8112279.

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Centralized/Cloud Radio Access Networks (C-RAN) are deployed in converged fixed-mobile networks to exploit the flexibility coming from joint application of Network Function Virtualization (NFV) and Software Defined Networking (SDN). In this context, optical links connecting C-RAN nodes, possibly based on the Ethernet standards, may carry traffic with different requirements in terms of latency and throughput. This paper considers the problem of traffic aggregation on C-RAN optical Ethernet links with latency control for fronthaul traffic and throughput capability for backhaul traffic. Integrated hybrid network technique is applied to show how time transparency can be enforced for Ethernet encapsulated Common Public Radio Interface (CPRI) traffic while allowing statistical multiplexing of backhaul traffic. Simulation results show the effectiveness of segmentation of backhaul traffic to allow exploitation of the available bandwidth even with high capacity CPRI options.

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7

V, Anuradha, and Shankar R. "Framework Enhancement for Common Public Radio Interface in SBTS." International Journal of Engineering & Technology 7, no. 3.12 (July 20, 2018): 38. http://dx.doi.org/10.14419/ijet.v7i3.12.15859.

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There is a rapid growth of mobile users so there are developing more number of features by a service provider to support more number of mobile users. The Common Public Radio Interface (CPRI) is an interface between the Radio Equipment Controller (REC) and Radio Equipment (RE) to support more number of users. This simplifies the overall architecture of radio base station. The radio base station is centralized and the radio heads are distributed in the environment. This radio heads supports the more number of users. The main aim of CPRI is to divide the packets into number of frames. The radio frame is divided into the hyper frames and this hyper frame is divided into the number of basic frame in which each basic frame is supported up to 16 words totally it supports up to 6,144 megabytes per second.

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8

Guo, Bin, Wei Cao, An Tao, and Dragan Samardzija. "LTE/LTE-A signal compression on the CPRI interface." Bell Labs Technical Journal 18, no. 2 (September 2013): 117–33. http://dx.doi.org/10.1002/bltj.21608.

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9

Arunjothi, R., T. Thirumurthy, P. V. Satheesh Kumar, G. K. Raja, and K. P. Meena. "Flame Retardancy of Instrumentation and Control Cables � CPRI�s Experience." Power Research - A Journal of CPRI 14, no. 2 (June 12, 2019): 121–25. http://dx.doi.org/10.33686/pwj.v14i2.144709.

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10

Si, Hongbo, Boon Loong Ng, Md Saifur Rahman, and Jianzhong Zhang. "A Novel and Efficient Vector Quantization Based CPRI Compression Algorithm." IEEE Transactions on Vehicular Technology 66, no. 8 (August 2017): 7061–71. http://dx.doi.org/10.1109/tvt.2017.2670561.

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11

Dong, Jian, and Jie Yang. "Cascaded Phase Modulation for AMCC Superimposition Toward MFH Employing CPRI." IEEE Photonics Journal 9, no. 4 (August 2017): 1–6. http://dx.doi.org/10.1109/jphot.2017.2720755.

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12

Chitimalla, Divya, Koteswararao Kondepu, Luca Valcarenghi, Massimo Tornatore, and Biswanath Mukherjee. "5G Fronthaul–Latency and Jitter Studies of CPRI Over Ethernet." Journal of Optical Communications and Networking 9, no. 2 (February 1, 2017): 172. http://dx.doi.org/10.1364/jocn.9.000172.

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13

Zeng, Huaiyu, Xiang Liu, Sharief Megeed, Naresh Chand, and Frank Effenberger. "Real-Time Demonstration of CPRI-Compatible Efficient Mobile Fronthaul Using FPGA." Journal of Lightwave Technology 35, no. 6 (March 15, 2017): 1241–47. http://dx.doi.org/10.1109/jlt.2017.2660484.

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14

Kaur, Gurjeet. "User's Attitude towards Electronic Information Resources at CPRI and CIHT, Jalandhar, Punjab." Library Progress (International) 38, no. 2 (2018): 211. http://dx.doi.org/10.5958/2320-317x.2018.00022.3.

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15

Wolf, L., and B. Goldberg. "Autistic Children Grow Up: An Eight to Twenty-Four Year Follow-Up Study." Canadian Journal of Psychiatry 31, no. 6 (August 1986): 550–56. http://dx.doi.org/10.1177/070674378603100613.

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Eighty questionnaires were sent to parents and/or caregivers of autistic persons diagnosed between 1960–73 at CPRI, a regional assessment and treatment centre. The objective was to determine their present place of residence, functioning ability, language development, program involvement, and seizure activity. The results of this study support evidence that more than 50 percent require long-term institutional care; almost one-third suffer epileptic seizures; there is a persistence of symptoms and difficulty in gaining useful speech; few live independently or are capable of employment.

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16

Valcarenghi, L., K. Kondepu, and P. Castoldi. "Time- Versus Size-Based CPRI in Ethernet Encapsulation for Next Generation Reconfigurable Fronthaul." Journal of Optical Communications and Networking 9, no. 9 (August 17, 2017): D64. http://dx.doi.org/10.1364/jocn.9.000d64.

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17

Kim, Sung-Man, Sil-Gu Mun, and Sang-Soo Lee. "Required Specification Analysis of CPRI Link of 4G Mobile Networks for Using WDM-PON Transmission." Journal of Korean Institute of Communications and Information Sciences 37, no. 7B (July 31, 2012): 499–504. http://dx.doi.org/10.7840/kics.2012.37.7b.499.

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18

Chisholm, Malcolm H., and Keith S. Kramer. "Site-selective hydrogenolysis, hydrogenation and alcoholysis involving the homometallic cluster [W6H5(CPri)(OPri)12]." Chemical Communications, no. 11 (1996): 1331. http://dx.doi.org/10.1039/cc9960001331.

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19

Srivastava, Gaurav Kumar, and M. S. R. Murthy Vemavarapu. "Drillability prediction in some metamorphic rocks using composite penetration rate index (CPRI) – An approach." International Journal of Mining Science and Technology 31, no. 4 (July 2021): 631–41. http://dx.doi.org/10.1016/j.ijmst.2021.05.010.

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20

Hailu, Dawit Hadush. "Performance Evaluation of Ethernet Network for Mobile Fronthual Networks." Indonesian Journal of Electrical Engineering and Computer Science 7, no. 1 (July 1, 2017): 287. http://dx.doi.org/10.11591/ijeecs.v7.i1.pp287-298.

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<p>Increasing mobile data traffic due to the rise of both smartphones and tablets has led to high-capacity demand of mobile data network. To meet the ever-growing capacity demand and reduce the cost of mobile network components, Cloud Radio Access Network (C-RAN) has emerged as a promising solution. In such network, the mobile operator’s Remote Radio Head (RRH) and Base Band Unit (BBU) are often separated and the connection between them has very tight timing and latency requirements imposed by Common Public Radio Interface (CPRI) and 3rd Generation Partnership Project (3GPP). This fronthaul connection is not yet provided by packet based network. To employ packet-based network for C-RAN fronthaul, the carried fronthaul traffic are needed to achieve the requirements of fronthaul streams. For this reason, the aim of this paper is focused on investigating and evaluating the feasibility of Ethernet networks for mobile fronthaul. The fronthaul requirements used to evaluate and investigate this network are maximum End to End (E2E) latency, Packet Loss Ratio (PLR) and Packet Delay Variation (PDV). The simulated results and numerical analysis confirm that the PDV and PLR of High Priority (HP) traffic in Ethernet network meet the requirements of mobile fronthaul using CPRI. However, the PDV of HP traffic meets the fronthaul network when the number of nodes in the Ethernet network is at most four. For Ethernet network, the number of nodes in the network limits the maximum separation distance between BBU and RRH (link length); for increasing the number of nodes, the link length decreases. Consequently, Radio over Ethernet (RoE) traffic should receive the priority and Quality of Service (QoS) HP can provide. On the other hand, Low Priority (LP) classes are not sensitive to QoS metrics and should be used for transporting time insensitive applications and services.</p>

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21

Love, Andrew J., Valérie Laval, Chiara Geri, Janet Laird, A. Deri Tomos, Mark A. Hooks, and Joel J. Milner. "Components of Arabidopsis Defense- and Ethylene-Signaling Pathways Regulate Susceptibility to Cauliflower mosaic virus by Restricting Long-Distance Movement." Molecular Plant-Microbe Interactions® 20, no. 6 (June 2007): 659–70. http://dx.doi.org/10.1094/mpmi-20-6-0659.

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We analyzed the susceptibility of Arabidopsis mutants with defects in salicylic acid (SA) and jasmonic acid (JA)/ethylene (ET) signaling to infection by Cauliflower mosaic virus (CaMV). Mutants cpr1-1 and cpr5-2, in which SA-dependent defense signaling is activated constitutively, were substantially more resistant than the wild type to systemic infection, implicating SA signaling in defense against CaMV. However, SA-deficient NahG, sid2-2, eds5-1, and pad4-1 did not show enhanced susceptibility. A cpr5 eds5 double mutant also was resistant, suggesting that resistance in cpr5 may function partially independently of SA. Treatment of cpr5 and cpr5 eds5, but not cpr1, with salicyl-hydroxamic acid, an inhibitor of alternative oxidase, partially restored susceptibility to wild-type levels. Mutants etr1-1, etr1-3, and ein2-1, and two mutants with lesions in ET/JA-mediated defense, eds4 and eds8, also showed reduced virus susceptibility, demonstrating that ET-dependent responses also play a role in susceptibility. We used a green fluorescent protein (GFP)-expressing CaMV recombinant to monitor virus movement. In mutants with reduced susceptibility, cpr1-1, cpr5-2, and etr1-1, CaMV-GFP formed local lesions similar to the wild type, but systemic spread was almost completely absent in cpr1 and cpr5 and was substantially reduced in etr1-1. Thus, mutations with enhanced systemic acquired resistance or compromised ET signaling show diminished long-distance virus movement.

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Zuehlke, Abbey D., Nicholas Wren, Victoria Tenge, and Jill L. Johnson. "Interaction of Heat Shock Protein 90 and the Co-chaperone Cpr6 with Ura2, a Bifunctional Enzyme Required for Pyrimidine Biosynthesis." Journal of Biological Chemistry 288, no. 38 (August 7, 2013): 27406–14. http://dx.doi.org/10.1074/jbc.m113.504142.

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The molecular chaperone heat shock protein 90 (Hsp90) is an essential protein required for the activity and stability of multiple proteins termed clients. Hsp90 cooperates with a set of co-chaperone proteins that modulate Hsp90 activity and/or target clients to Hsp90 for folding. Many of the Hsp90 co-chaperones, including Cpr6 and Cpr7, contain tetratricopeptide repeat (TPR) domains that bind a common acceptor site at the carboxyl terminus of Hsp90. We found that Cpr6 and Hsp90 interacted with Ura2, a protein critical for pyrimidine biosynthesis. Mutation or inhibition of Hsp90 resulted in decreased accumulation of Ura2, indicating it is an Hsp90 client. Cpr6 interacted with Ura2 in the absence of stable Cpr6-Hsp90 interaction, suggesting a direct interaction. However, loss of Cpr6 did not alter the Ura2-Hsp90 interaction or Ura2 accumulation. The TPR domain of Cpr6 was required for Ura2 interaction, but other TPR containing co-chaperones, including Cpr7, failed to interact with Ura2 or rescue CPR6-dependent growth defects. Further analysis suggests that the carboxyl-terminal 100 amino acids of Cpr6 and Cpr7 are critical for specifying their unique functions, providing new information about this important class of Hsp90 co-chaperones.

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23

Basu, P. S., Ashoo Sharma, I. D. Garg, and N. P. Sukumaran. "Tuber sink modifies photosynthetic response in potato under water stress1Publication No. 1408, CPRI, Shimla.1." Environmental and Experimental Botany 42, no. 1 (August 1999): 25–39. http://dx.doi.org/10.1016/s0098-8472(99)00017-9.

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24

de la Oliva, Antonio, Jose Alberto Hernandez, David Larrabeiti, and Arturo Azcorra. "An overview of the CPRI specification and its application to C-RAN-based LTE scenarios." IEEE Communications Magazine 54, no. 2 (February 2016): 152–59. http://dx.doi.org/10.1109/mcom.2016.7402275.

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25

Rebola, João L., Adolfo V. T. Cartaxo, and André S. Marques. "10 Gbps CPRI signals transmission impaired by intercore crosstalk in 5G network fronthauls with multicore fibers." Photonic Network Communications 37, no. 3 (January 12, 2019): 409–20. http://dx.doi.org/10.1007/s11107-019-00828-0.

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26

Dolinski, Kara J., Maria E. Cardenas, and Joseph Heitman. "CNS1 Encodes an Essential p60/Sti1 Homolog in Saccharomyces cerevisiae That Suppresses Cyclophilin 40 Mutations and Interacts with Hsp90." Molecular and Cellular Biology 18, no. 12 (December 1, 1998): 7344–52. http://dx.doi.org/10.1128/mcb.18.12.7344.

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ABSTRACT Cyclophilins are cis-trans-peptidyl-prolyl isomerases that bind to and are inhibited by the immunosuppressant cyclosporin A (CsA). The toxic effects of CsA are mediated by the 18-kDa cyclophilin A protein. A larger cyclophilin of 40 kDa, cyclophilin 40, is a component of Hsp90-steroid receptor complexes and contains two domains, an amino-terminal prolyl isomerase domain and a carboxy-terminal tetratricopeptide repeat (TPR) domain. There are two cyclophilin 40 homologs in the yeast Saccharomyces cerevisiae, encoded by the CPR6 and CPR7 genes. Yeast strains lacking the Cpr7 enzyme are viable but exhibit a slow-growth phenotype. In addition, we show here that cpr7 mutant strains are hypersensitive to the Hsp90 inhibitor geldanamycin. When overexpressed, the TPR domain of Cpr7 alone complements both cpr7 mutant phenotypes, while overexpression of the cyclophilin domain of Cpr7, full-length Cpr6, or human cyclophilin 40 does not. The open reading frame YBR155w, which has moderate identity to the yeast p60 homologSTI1, was isolated as a high-copy-number suppressor of thecpr7 slow-growth phenotype. We show that this Sti1 homolog Cns1 (cyclophilin seven suppressor) is constitutively expressed, essential, and found in protein complexes with both yeast Hsp90 and Cpr7 but not with Cpr6. Cyclosporin A inhibited Cpr7 interactions with Cns1 but not with Hsp90. In summary, our findings identify a novel component of the Hsp90 chaperone complex that shares function with cyclophilin 40 and provide evidence that there are functional differences between two conserved sets of Hsp90 binding proteins in yeast.

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Eramo, Vincenzo, Marco Listanti, Francesco Lavacca, and Paola Iovanna. "Dimensioning Models of Optical WDM Rings in Xhaul Access Architectures for the Transport of Ethernet/CPRI Traffic." Applied Sciences 8, no. 4 (April 12, 2018): 612. http://dx.doi.org/10.3390/app8040612.

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28

Kemp, Laura R., Mark S. Dunstan, Karl Fisher, Jim Warwicker, and David Leys. "The transcriptional regulator CprK detects chlorination by combining direct and indirect readout mechanisms." Philosophical Transactions of the Royal Society B: Biological Sciences 368, no. 1616 (April 19, 2013): 20120323. http://dx.doi.org/10.1098/rstb.2012.0323.

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The transcriptional regulator CprK controls the expression of the reductive dehalogenase CprA in organohalide-respiring bacteria. Desulfitobacterium hafniense CprA catalyses the reductive dechlorination of the terminal electron acceptor o -chlorophenol acetic acid, generating the phenol acetic acid product. It has been shown that CprK has ability to distinguish between the chlorinated CprA substrate and the de-halogenated end product, with an estimated an estimated 10 4 -fold difference in affinity. Using a green fluorescent protein GFP UV -based transcriptional reporter system, we establish that CprK can sense o -chlorophenol acetic acid at the nanomolar level, whereas phenol acetic acid leads to transcriptional activation only when approaching micromolar levels. A structure–activity relationship study, using a range of o -chlorophenol acetic-acid-related compounds and key CprK mutants, combined with p K a calculations on the effector binding site, suggests that the sensitive detection of chlorination is achieved through a combination of direct and indirect readout mechanisms. Both the physical presence of the bulky chloride substituent as well as the accompanying electronic effects lowering the inherent phenol p K a are required for high affinity. Indeed, transcriptional activation by CprK appears strictly dependent on establishing a phenolate–K133 salt bridge interaction, rather than on the presence of a halogen atom per se . As K133 is strictly conserved within the CprK family, our data suggest that physiological function and future applications in biosensing are probably restricted to phenolic compounds.

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Mani, Darjusch, and Heinrich Vahrenkamp. "Cluster-Expansion mit CpRh- und CpIr-Bausteinen." Chemische Berichte 119, no. 12 (December 1986): 3649–71. http://dx.doi.org/10.1002/cber.19861191213.

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Clarke, Joseph D., Nicole Aarts, Bart J. Feys, Xinnian Dong, and Jane E. Parker. "Constitutive disease resistance requires EDS1 in the Arabidopsis mutants cpr1 and cpr6 and is partially EDS1 -dependent in cpr5." Plant Journal 26, no. 4 (May 2001): 409–20. http://dx.doi.org/10.1046/j.1365-313x.2001.2641041.x.

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Tenge, Victoria R., Abbey D. Zuehlke, Neelima Shrestha, and Jill L. Johnson. "The Hsp90 Cochaperones Cpr6, Cpr7, and Cns1 Interact with the Intact Ribosome." Eukaryotic Cell 14, no. 1 (November 7, 2014): 55–63. http://dx.doi.org/10.1128/ec.00170-14.

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ABSTRACT The abundant molecular chaperone Hsp90 is essential for the folding and stabilization of hundreds of distinct client proteins. Hsp90 is assisted by multiple cochaperones that modulate Hsp90's ATPase activity and/or promote client interaction, but the in vivo functions of many of these cochaperones are largely unknown. We found that Cpr6, Cpr7, and Cns1 interact with the intact ribosome and that Saccharomyces cerevisiae lacking CPR7 or containing mutations in CNS1 exhibited sensitivity to the translation inhibitor hygromycin. Cpr6 contains a peptidyl-prolyl isomerase (PPIase) domain and a tetratricopeptide repeat (TPR) domain flanked by charged regions. Truncation or alteration of basic residues near the carboxy terminus of Cpr6 disrupted ribosome interaction. Cns1 contains an amino-terminal TPR domain and a poorly characterized carboxy-terminal domain. The isolated carboxy-terminal domain was able to interact with the ribosome. Although loss of CPR6 does not cause noticeable growth defects, overexpression of CPR6 results in enhanced growth defects in cells expressing the temperature-sensitive cns1-G90D mutation (the G-to-D change at position 90 encoded by cns1 ). Cpr6 mutants that exhibit reduced ribosome interaction failed to cause growth defects, indicating that ribosome interaction is required for in vivo functions of Cpr6. Together, these results represent a novel link between the Hsp90 molecular-chaperone machine and protein synthesis.

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32

Bachmann, Sebastian, Björn Gernert, and Dietmar Stalke. "Solution structures of alkali metal cyclopentadienides in THF estimated by ECC-DOSY NMR-spectroscopy (incl. software)." Chemical Communications 52, no. 87 (2016): 12861–64. http://dx.doi.org/10.1039/c6cc07273a.

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In this paper we present the aggregational motifs of the widely used alkali-metal cyclopentadienides (CpLi, CpNa, CpK, CpRb, CpCs) in THF-d₈ solution estimated by ECC-DOSY NMR spectroscopy.

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Satish, K. H., and M. Rizwana. "E-Office: A Recent Proposal in Government R&D Institution: An Empirical Study." Journal of Computational and Theoretical Nanoscience 17, no. 9 (July 1, 2020): 4446–50. http://dx.doi.org/10.1166/jctn.2020.9094.

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The continual advancement of new technologies have become prominent for Information and Communication Technology (ICT) to be applied and adopted in e-government initiatives. The implementation of ICT and e-services has been adopted by many countries, but implementation rate varies from country to country. The main objective being providing greater transparency, efficiency, rendering quality service. Several government institutions have undertaken e-governance initiatives at multiple levels in the recent past and one such sector taken for study being the Indian Power Sector system which is growing at a very rapid phase, having progressed with its huge amount of infrastructure made available to the research community. E-office implementation in power sector is very crucial for improving efficiency and commitment of Government sector for sustained growth of economy. The present study highlights the implementation and utilization of E-office by the employees of “Central Power Research Institute” (CPRI), a R&D Organisation, an Autonomous society under Ministry of Power, Government of India. A pilot study of 30 employees having different backgrounds was carried out. The study findings highlights practicality of usage in E-office.

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Sappa, Enrico, Giovanni Predieri, Antonio Tiripicchio, and Marisa Tiripicchio Camellini. "The synthesis and crystal structure of (η5-C5H5)NiOs3(μ-H)3(CO)8(Ph2PCCPri) and of (η5-C5H5)NiOs3(μ-H)3(CO)8{(Ph2PCCPri)Co2(CO)6}(σ-P,η2-C2). The reaction of unsaturated “linear” ligands on clusters with metal carbonyl species." Journal of Organometallic Chemistry 297, no. 1 (December 1985): 103–15. http://dx.doi.org/10.1016/0022-328x(85)80401-0.

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Ishimura, Shota, Abdelmoula Bekkali, Kazuki Tanaka, Kosuke Nishimura, and Masatoshi Suzuki. "1.032-Tb/s CPRI-Equivalent Rate IF-Over-Fiber Transmission Using a Parallel IM/PM Transmitter for High-Capacity Mobile Fronthaul Links." Journal of Lightwave Technology 36, no. 8 (April 15, 2018): 1478–84. http://dx.doi.org/10.1109/jlt.2017.2787151.

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Jirage, Dayadevi, Nan Zhou, Bret Cooper, Joseph D. Clarke, Xinnian Dong, and Jane Glazebrook. "Constitutive salicylic acid-dependent signaling in cpr1 and cpr6 mutants requires PAD4." Plant Journal 26, no. 4 (May 2001): 395–407. http://dx.doi.org/10.1046/j.1365-313x.2001.2641040.x.

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SL, Erayya, Nandani Shukla, Kahkashan Arzoo, and J. Kumar. "Mass screening of Trichoderma spp. for their antagonism against some plant pathogenic oomycetes fungi." Journal of Applied and Natural Science 10, no. 3 (September 1, 2018): 813–17. http://dx.doi.org/10.31018/jans.v10i3.1718.

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In vitro efficacy of twenty five Trichoderma isolates (twenty were TCMS series viz., TCMS 2, 4, 5, 12, 14a, 14b, 15, 16, 24, 32, 34, 36, 43, 60, 62, 64, 65, 72, 85 and 93, and five Th series; Th 1, 3, 14, 19 and 32) were ascertained for their antagonistic activity against few major plant pathogenic oomycetes namely, Phytophthora infestans, P. parasitica and Pythium aphenidermatum using dual culture technique. P. infestans was isolated from infected potato leaves and Pythium aphenidermatum from infected brinjal. P. parasitica culture was collected from Central Potato Research Institute (CPRI), Simla. The present study was conducted at Biological Control Laboratory, Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar. All the 25 Trichodrma isolates were found significantly effective against the test pathogens. TCMS-36 and TCMS-72 were found highly effective against P. aphinidermatum with 59.57 per cent inhibition of radial growth of the fungus. Maximum reduction in mycelial growth of P. infestans was recorded with isolate TCMS-64 (60.40%) followed by TCMS-65 (59.41%), TCMS-34 (58.42%), TCMS-24, TCMS-43 and TCMS-93 with 57.43 per cent inhibition. While, maximum inhibition of P. parasitica was recorded with TCMS-4 (92.75%) followed by TCMS-36 (92.23%), TCMS-2 (91.71%), TCMS-14a (91.17%) and TCMS-32 (90.67%). The selected potential isolates may be applied to sustainable and eco-friendly management of many major crop diseases caused by the oomycetes and other fungi.

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Sappa, Enrico, Danielle Belletti, Antonio Tiripicchio, and Marisa Tiripicchio Camellini. "Synthesis and crystal structure of (η5-C5H5)NiFe3(CO)7(μ-PPh2)(μ4,η2-HCCPri): a butterfly cluster with an unprecedented metal core stoichiometry." Journal of Organometallic Chemistry 359, no. 3 (January 1989): 419–28. http://dx.doi.org/10.1016/0022-328x(89)88102-1.

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Marsh, James A., Helen M. Kalton, and Richard F. Gaber. "Cns1 Is an Essential Protein Associated with the Hsp90 Chaperone Complex in Saccharomyces cerevisiae That Can Restore Cyclophilin 40-Dependent Functions in cpr7ΔCells." Molecular and Cellular Biology 18, no. 12 (December 1, 1998): 7353–59. http://dx.doi.org/10.1128/mcb.18.12.7353.

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ABSTRACT Saccharomyces cerevisiae harbors two cyclophilin 40-type enzymes, Cpr6 and Cpr7, which are components of the Hsp90 molecular chaperone machinery. Cpr7 is required for normal growth and is required for maximal activity of heterologous Hsp90-dependent substrates, including glucocorticoid receptor (GR) and the oncogenic tyrosine kinase pp60v-src . In addition, it has recently been shown that Cpr7 plays a major role in negative regulation of the S. cerevisiae heat shock transcription factor (HSF). To better understand functions associated with Cpr7, a search was undertaken for multicopy suppressors of the cpr7Δ slow-growth phenotype. The screen identified a single gene, designatedCNS1 (for cyclophilin seven suppressor), capable of suppressing the cpr7Δ growth defect. Overexpression ofCNS1 in cpr7Δ cells also largely restored GR activity and negative regulation of HSF. In vitro protein retention experiments in which Hsp90 heterocomplexes were precipitated resulted in coprecipitation of Cns1. Interaction between Cns1 and the carboxy terminus of Hsp90 was also shown by two-hybrid analysis. The functional consequences of CNS1 overexpression and its physical association with the Hsp90 machinery indicate that Cns1 is a previously unidentified component of molecular chaperone complexes. Thus far, Cns1 is the only tetratricopeptide repeat-containing component of Hsp90 heterocomplexes found to be essential for cell viability under all conditions tested.

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Wang, Weiqi, Derrick Lai, Abbas Abid, Suvadip Neogi, Xuping Xu, and Chun Wang. "Effects of Steel Slag and Biochar Incorporation on Active Soil Organic Carbon Pools in a Subtropical Paddy Field." Agronomy 8, no. 8 (August 2, 2018): 135. http://dx.doi.org/10.3390/agronomy8080135.

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Industrial wastes and agricultural byproducts are increasingly used in crop production as fertilizers, but their impacts on soil carbon (C) sequestration remain poorly understood. The aim of this study was to examine the effects of applying steel slag (SS), biochar (B), and a combination of these two materials (SS + B) on total soil organic C (SOC), active SOC fractions, and C pool management index (CPMI) in a subtropical paddy field in China. The treatments were applied at a rate of 8 t ha−1 to rice at the two (early and late) crop seasons in 2015. The SOC concentrations in the top 30 cm soils in the SS + B treatments were 28.7% and 42.2% higher in the early and late crops, respectively, as compared to the controls (p < 0.05). SOC was positively correlated with soil C:N ratio across the two crop seasons (r = 0.92–0.97, p < 0.01). As compared to the control, SS + B treatment had significantly higher carbon pool index (CPI) in both early (22.4%) and late (40.1%) crops. In the early crop, the C pool activity index (CPAI) was significantly lower in B and SS + B treatments by over 50% than in the control, while the soil C pool management index (CPMI) in the SS, B, and SS + B treatments was lower than that in the control by 36.7%, 41.6%, and 45.4%, respectively. In contrast, in the late crop, no significant differences in CPAI and CPMI were observed among the treatments. Our findings suggest that the addition of steel slag and biochar in subtropical paddy fields could decrease active SOC pools and enhance soil C sequestration only in the early crop, but not the late crop.

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Hailu, Dawit Hadush, Gebrehiwet Gebrekrstos Lema, Gebremichael T. Tesfamariam, and Tole Sutikno. "Integrated hybrid optical networking for 5G access networks." Indonesian Journal of Electrical Engineering and Computer Science 17, no. 3 (March 1, 2020): 1647. http://dx.doi.org/10.11591/ijeecs.v17.i3.pp1647-1656.

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Today, deployment of optical fiber has offered large transmission capacity which cannot be efficiently utilized by the electronic switches. Rather, Integrated Hybrid Optical Network (IHON) is a promising approach which combines both packet and circuit switching techniques. As a result, it achieves efficient utilization of the bulk capacity and guarantees absolute Quality of Service (QoS) by optimizing the advantages of the two switching schemes while diminishing their disadvantages. Transpacket has developed a Fusion node implementing IHON principles in Ethernet for the data plane. Hence, this paper investigates and evaluates IHON network for 5G access networks. The simulated results and numerical analysis confirm that the Packet Delay Variation (PDV), Delay and Packet Loss Ratio (PLR) of Guaranteed Service Transport. (GST) traffic in IHON network met the requirements of 5G mobile fronthaul using CPRI. The number of nodes in the network limits the maximum separation distance between Base Band Unit. (BBU) and Remote Radio Head (RRH), link length; for increasing the number of nodes, the link length decreases. In addition to this, we verified how the leftover capacity of fusion node can be used to carry the low priority packets and how the GST traffic can have deterministic characteristics on a single wavelength by delaying it with Fixed Delay Line (FDL). For example, for L<sup>SM</sup><sub>1GE</sub>=0.3 the added Statistical Multiplexing (SM) traffic increases the 10GE wavelength utilization up to 89% without any losses and with SM PLR=1<em>E</em><sup>−03</sup> up to 92% utilization.

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Chakraborty, S., U. Haldar, A. K. Bera, A. K. Pal, S. Bhattacharya, S. Ghosh, B. P. Mukhopadhyay, and Asok Banerjee. "Recognition and Stabilization of A Unique CPRI—Structural Motif in Cucurbitaceae Family Trypsin Inhibitor Peptides: Molecular Dynamics Based Homology Modeling Using the X-ray Structure of MCTI-II." Journal of Biomolecular Structure and Dynamics 18, no. 4 (February 1, 2001): 569–77. http://dx.doi.org/10.1080/07391102.2001.10506689.

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Verma, Shiv Kumar, Anand Kumar, Moti Lal, and Mira Debnath. "Biodegradation of Synthetic Dye by Endophytic Fungal Isolate in Calotropis procera Root." International Journal of Applied Sciences and Biotechnology 3, no. 3 (September 25, 2015): 373–80. http://dx.doi.org/10.3126/ijasbt.v3i3.13136.

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In this study, based on colony morphology characteristics, a total of 19 fungal endophytes were isolated from root of Calotropis Procera a traditional Indian medicinal plant. All fungal isolates were screened for their dye degradation ability. The dyes used as test dyes were Rose Bengal (RB), azo dye Methyl Red (MR), Coomassie Brilliant Blue (CBB) and Methylene Blue (MB) and the concentration of each dye in the experiment was kept 100mg/L. Among the 19 fungal endophytic isolates (CPR1-CPR19), only one isolate CPR4 showed strong dye decolourization capability against all the four test dye. Dye decolourization ability by the isolate CPR4 was determined to be 97.4%, 87%, 65% and 45% for Rose Bengal (RB), Methyl Red (MR), Coomassie Brilliant Blue (CBB) and Methylene Blue (MB) respectively. Complete colour decolourization was observed with rose Bengal followed by Methyl Red. Glucose minimal medium was used for liquid and solid culture of fungal isolates. Fungal biomass production in the presence of four test dye was studied and compare with control culture of fungal endophytes. Effect of temperature, pH, stationary and agitation conditions on dye degradation was also studied.Int J Appl Sci Biotechnol, Vol 3(3): 373-380

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Mayr, Christian, Klaus Richter, Hauke Lilie, and Johannes Buchner. "Cpr6 and Cpr7, Two Closely Related Hsp90-associated Immunophilins fromSaccharomyces cerevisiae, Differ in Their Functional Properties." Journal of Biological Chemistry 275, no. 44 (August 14, 2000): 34140–46. http://dx.doi.org/10.1074/jbc.m005251200.

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Wubben, Martin John Evers, Jing Jin, and Thomas Josef Baum. "Cyst Nematode Parasitism of Arabidopsis thaliana Is Inhibited by Salicylic Acid (SA) and Elicits Uncoupled SA-Independent Pathogenesis-Related Gene Expression in Roots." Molecular Plant-Microbe Interactions® 21, no. 4 (April 2008): 424–32. http://dx.doi.org/10.1094/mpmi-21-4-0424.

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Compatible plant–nematode interactions involve the formation of an elaborate feeding site within the host root that requires the evasion of plant defense mechanisms by the parasite. Little is known regarding plant defense signaling pathways that limit nematode parasitism during a compatible interaction. Therefore, we utilized Arabidopsis thaliana mutants perturbed in salicylic acid (SA) biosynthesis or signal transduction to investigate the role of SA in inhibiting parasitism by the beet cyst nematode Heterodera schachtii. We determined that SA-deficient mutants (sid2-1, pad4-1, and NahG) exhibited increased susceptibility to H. schachtii. In contrast, SA-treated wild-type plants showed decreased H. schachtii susceptibility. The npr1-2 and npr1-3 mutants, which are impaired in SA signaling, also showed increased susceptibility to H. schachtii, whereas the npr1-suppressor mutation sni1 showed decreased susceptibility. Constitutive pathogenesis-related (PR) gene-expressing mutants (cpr1 and cpr6) did not show altered susceptibility to H. schachtii; however, constitutive PR gene expression was restricted to cpr1 shoots with wild-type levels of PR-1 transcript present in cpr1 roots. Furthermore, we determined that H. schachtii infection elicits SA-independent PR-2 and PR-5 induction in wild-type roots, while PR-1 transcript and total SA levels remained unaltered. This was in contrast to shoots of infected plants where PR-1 transcript abundance and total SA levels were elevated. We conclude that SA acts via NPR1 to inhibit nematode parasitism which, in turn, is negatively regulated by SNI1. Our results show an inverse correlation between root basal PR-1 expression and plant susceptibility to H. schachtii and suggest that successful cyst nematode parasitism may involve a local suppression of SA signaling in roots.

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Hailu, Dawit Hadush. "QoS Performance of Integrated Hybrid Optical Network in Mobile Fronthual Networks." Indonesian Journal of Electrical Engineering and Computer Science 7, no. 1 (July 1, 2017): 189. http://dx.doi.org/10.11591/ijeecs.v7.i1.pp189-204.

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<p>Cloud Radio Access Network (C-RAN) has emerged as a promising solution to meet the ever-growing capacity demand and reduce the cost of mobile network components. In such network, the mobile operator’s Remote Radio Head (RRH) and Base Band Unit (BBU) are often separated and the connection between them has very tight timing and latency requirements. To employ packet-based network for C-RAN fronthaul, the carried fronthaul traffic are needed to achieve the requirements of fronthaul streams. For this reason, the aim of this paper is focused on investigating and evaluating the feasibility of Integrated Hybrid Optical Network (IHON) networks for mobile fronthaul. TransPacket AS (www.transpacket.com) develops a fusion switching that efficiently serves both Guaranteed Service Transport (GST) traffic with absolute priority and packet switched Statistical Multiplexing (SM) best effort traffic. We verified how the leftover capacity of fusion node can be used to carry the low priority packets and how the GST traffic can have deterministic characteristics on a single wavelength by delaying it with Fixed Delay Line (FDL). For example, for L<sub>1GE </sub><sup>SM</sup> =0.3 the added SM traffic increases the 10GE wavelength utilization up to 89% without any losses and with SM PLR=1E<sup>-03</sup> up to 92% utilization. The simulated results and numerical analysis confirm that the PDV and PLR of GST traffic in Ethernet network meet the requirements of mobile fronthaul using CPRI. For Ethernet network, the number of nodes in the network limits the maximum separation distance between BBU and RRH (link length); for increasing the number of nodes, the link length decreases. Consequently, Radio over Ethernet (RoE) traffic should receive the priority and Quality of Service (QoS) HP can provide. On the other hand, Low Priority (LP) classes are not sensitive to QoS metrics and should be used for transporting time insensitive applications and services.</p>

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Lasschuijt, Marlou P., Monica Mars, Cees de Graaf, and Paul A. M. Smeets. "Endocrine Cephalic Phase Responses to Food Cues: A Systematic Review." Advances in Nutrition 11, no. 5 (June 9, 2020): 1364–83. http://dx.doi.org/10.1093/advances/nmaa059.

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ABSTRACT Cephalic phase responses (CPRs) are conditioned anticipatory physiological responses to food cues. They occur before nutrient absorption and are hypothesized to be important for satiation and glucose homeostasis. Cephalic phase insulin responses (CPIRs) and pancreatic polypeptide responses (CPPPRs) are found consistently in animals, but human literature is inconclusive. We performed a systematic review of human studies to determine the magnitude and onset time of these CPRs. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to develop a search strategy. The terms included in the search strategy were cephalic or hormone response or endocrine response combined with insulin and pancreatic polypeptide (PP). The following databases were searched: Scopus (Elsevier), Science Direct, PubMed, Google Scholar, and The Cochrane Library. Initially, 582 original research articles were found, 50 were included for analysis. An insulin increase (≥1μIU/mL) was observed in 41% of the treatments (total n = 119). In 22% of all treatments the increase was significant from baseline. The median (IQR) insulin increase was 2.5 (1.6–4.5) μIU/mL, 30% above baseline at 5± 3 min after food cue onset (based on study treatments that induced ≥1 μIU/mL insulin increase). A PP increase (>10 pg/mL) was found in 48% of the treatments (total n = 42). In 21% of the treatments, the increase was significant from baseline. The median (IQR) PP increase was 99 (26–156) pg/mL, 68% above baseline at 9± 4 min after food cue onset (based on study treatments that induced ≥1 μIU/mL insulin increase). In conclusion, CPIRs are small compared with spontaneous fluctuations. Although CPPPRs are of a larger magnitude, both show substantial variation in magnitude and onset time. We found little evidence for CPIR or CPPPR affecting functional outcomes, that is, satiation and glucose homeostasis. Therefore, CPRs do not seem to be biologically meaningful in daily life.

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Baimbridge, CW, RS Dickson, GD Fallon, I. Grayson, RJ Nesbit, and J. Weigold. "Some Substituent Effects in the Formation of Binuclear Metalladienes and 1,2-Dimetallacycloheptadienones From Reactions Between (η-C5H5)2Rh2(μ-CO)(CF3C2CF3) and Alkynes." Australian Journal of Chemistry 39, no. 8 (1986): 1187. http://dx.doi.org/10.1071/ch9861187.

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The reaction between (η-C5H5)2Rh2(CO)(CF3C2CF3)(1) and alkynes, RC=CR′, can proceed by two alternative pathways. One gives the complexes (η- C5H5)2Rh2{C4(CF3)2RR′CO} (2) in which a pentadienone unit bridges the Rh - Rh bond. The other produces the binuclear metalladiene complexes (η-C5H5)2Rh2{C4(CF3)2RR?} (3) plus the dicarbonyl complex (η-C5H5)2Rh2(CO)2(CF3C2CF3) (4). The formation of (2) is strongly favoured with the dialkylacetylenes BuC≡CBu and MeC =CR (R = Et, Pri, But). With the unsymmetrical acetylenes, two isomers of (2) are isolated. The tendency to form (3) + (4) increases when there are electron-withdrawing substituents such as CN on the alkyne, and becomes dominant when the alkyne is polar (e.g. MeC≡CR with R = Ph, CF3 or CO2Me). The reactions with MeC≡CR are highly regioselective, with only one of the two possible regioisomers being isolated in each case. This has the methyl group in the 3-position of the metalladiene ring. Unexpected products formed in the reactions with MeC≡Cet and MeC≡CPri have been characterized by X-ray crystallography. One is a 1,2- dimetallacycloheptadienone complex, (η-C5H5)2Rh2{C4(CF3)2Me(CMe2OH)CO}. The compound crystallizes with 16 molecules in the orthorhombic space group Fdd2 in a unit cell of dimensions a 31.728(15), b 27.923(14), c 9.275(5) Ǻ. The structure was solved by heavy atom methods and refined to R 0.037 based on 2644 observed reflections. The CMe2(OH) group is adjacent to the carbonyl in the bridging group. The other is a binuclear metalladiene complex (η-C5H5)2Rh2{C4(CF3)2Me( COMe )}. It crystallizes with eight molecules in the orthorhombic space group P bca in a unit cell of dimensions a 17.349(8), b 15.819(8), c 13.720(7) Ǻ. The structure was solved by heavy atom methods and refined to R 0.053 based on 3045 observed reflections above background. The acyl group is adjacent to the metal in the metalladiene ring. These two complexes are formed from (1) and impurities present in the alkynes.

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Verma, Shiv Kumar, Moti Lal, and Mira Debnath. "Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root." Asian Journal of Pharmaceutical and Clinical Research 10, no. 4 (April 1, 2017): 225. http://dx.doi.org/10.22159/ajpcr.2017.v10i4.16631.

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Objective:To study the antimicrobial activity of crud ethylacetate extract from endophytic fungus in Calotropis procera root.Methods:Endophytic fungus was screened for production of antimicrobial metablites.fermentation was carried out in 500 ml Erlenmeyer flask. Disc diffusion method was used to test antimicrobial activity of crude extract using chloramphenicol sulphate and Flucnazole as positive controleRessults: A total of fourteen Endophytic fungi were isolated (CPR1- CPR14). Among these fourteen isolates, CPR5 was found to show maximum antimicrobial activity, in compare to other isolates, against gram positive, gram negative bacteria and fungi. The antimicrobial activity was tested against Escherichia coli. Pseudomonas aeroginosa, Bacillus subtilis, Staphylococcus aureus, Ralstonia solanacearum, Xanthomonas oryzae, Penicillium chrysogenum, Candida albicans, Phoma exigua, Sclerotium rolfsii and Sclerotinia scleratiourum. Minimum inhibitory concentration (MIC) of crude extract against test microorganisms was determined. Fungus was identified as aspergillus sp. Prduction parameters (Temprature, pH, Carbon source, Nitrgen surce, Sdium Chlride concentration) were optimized.Conclusion: Crud extract produced by the isolated endophytic fungus could be an important source of broad spectrum antimicrobial metabolites. Keywords: Endophytic fungi, Antimicrobial metabolites, Process optimization, Calotropis procera, Inhibition zone.

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Gulliford, Martin C., Xiaohui Sun, Thamina Anjuman, Eleanor Yelland, and Tarita Murray-Thomas. "Comparison of antibiotic prescribing records in two UK primary care electronic health record systems: cohort study using CPRD GOLD and CPRD Aurum databases." BMJ Open 10, no. 6 (June 2020): e038767. http://dx.doi.org/10.1136/bmjopen-2020-038767.

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ObjectivesWe aimed to evaluate recording of antibiotic prescribing from two primary care electronic health record systems.DesignCohort study.SettingUK general practices contributing to the Clinical Practice Research Datalink (CPRD) databases: CPRD GOLD (Vision data) and CPRD Aurum (EMIS data). English CPRD GOLD general practices were analysed as a subgroup, as all CPRD Aurum practices were located in England.Participants158 305 patients were randomly sampled from CPRD Aurum and 160 394 from CPRD GOLD.Outcome measuresAntibiotic prescriptions in 2017 were identified. Age-standardised and sex-standardised antibiotic prescribing rates per 1000 person years were calculated. Prescribing of individual antibiotic products and associated medical diagnoses was evaluated.ResultsThere were 101 360 antibiotic prescriptions at 883 CPRD Aurum practices and 112 931 prescriptions at 290 CPRD GOLD practices, including 112 general practices in England. The age-standardised and sex-standardised antibiotic prescribing rate in 2017 was 512.6 (95% CI 510.4 to 514.9) per 1000 person years in CPRD Aurum and 584.3 (582.1 to 586.5) per 1000 person years in CPRD GOLD (505.2 (501.6 to 508.9) per 1000 person years if restricted to practices in England). The 25 most frequently prescribed antibiotic products were similar in both databases. One or more medical codes were recorded on the same date as an antibiotic prescription for 72 989 (74%) prescriptions in CPRD Aurum, 84 756 (78%) in CPRD GOLD and 28 471 (78%) for CPRD GOLD in England. Skin, respiratory and genitourinary tract infections were recorded for 39 035 (40%) prescriptions in CPRD Aurum, 41 326 (38%) in CPRD GOLD, with 15 481 (42%) in English CPRD GOLD practices only.ConclusionEstimates for antibiotic prescribing and infection recording were broadly similar in both databases suggesting similar recording across EMIS and Vision systems. Future research on antimicrobial stewardship can also be conducted using primary care data in CPRD Aurum.

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