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Journal articles on the topic 'CPV-2b'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Cavalli, Alessandra, Vito Martella, Costantina Desario, et al. "Evaluation of the Antigenic Relationships among Canine Parvovirus Type 2 Variants." Clinical and Vaccine Immunology 15, no. 3 (2007): 534–39. http://dx.doi.org/10.1128/cvi.00444-07.

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ABSTRACT The antigenic relationships among the original canine parvovirus type 2 (CPV-2) and the variants CPV-2a, -2b, and -2c were evaluated. Cross-antigenic evaluation revealed clear differences among the CPV variants, which were more appreciable by serum neutralization (SN) than by hemagglutination inhibition. Antigenic differences were found mostly between the original CPV-2 and the variants, but they were also observed among the variants CPV-2a, -2b, and -2c. The variant CPV-2c exhibited a unique antigenic pattern, since it was poorly recognized by the sera of animals immunized with CPV-2

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2

Inthong, N., S. Kaewmongkol, N. Meekhanon, K. Sirinarumitr, and T. Sirinarumitr. "Dynamic evolution of canine parvovirus in Thailand." February-2020 13, no. 2 (2020): 245–55. http://dx.doi.org/10.14202/vetworld.2020.245-255.

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Background and Aim: According to the previous study, the circulating canine parvovirus (CPV) in Thailand is 2a and 2b. Nowadays, CPV mutants, including CPV-2c, have been identified in many parts of the world. This study aimed to investigate the genetic diversity of the circulating CPV in Thailand. Materials and Methods: Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were an

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Mandawat, Saksham, Rashmi Singh, Nazeer Mohammed, et al. "Comparative Pathophysiology of Canine Parvovirus-2 Variants in Naturally Infected Dogs." Asian Journal of Research in Biochemistry 15, no. 3 (2025): 137–44. https://doi.org/10.9734/ajrb/2025/v15i3395.

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Canine Parvovirus-2 (CPV-2) is a highly lethal virus that affects the canid family. It targets rapidly dividing cells in organs such as the intestines, thymus, lymph nodes, bone marrow, and heart, often resulting in multi-organ failure.This study aimed to evaluate the impact of CPV-2 variants on clinico-physiological, haematological and biochemical parameters in dogs. Two hundred faecal and blood samples from symptomatic dogs, along with 10 healthy controls, were collected at VCC, PGIVER, Jaipur, from July 2024 to December 2024. PCR identified 166 CPV-2 positive cases, comprising 147 CPV-2a, 1

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Alexis, Véliz-Ahumada, Vidal Sonia, Siel Daniela, et al. "Molecular Analysis of Full-Length VP2 of Canine Parvovirus Reveals Antigenic Drift in CPV-2b and CPV-2c Variants in Central Chile." Animals 11, no. 8 (2021): 2387. http://dx.doi.org/10.3390/ani11082387.

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Canine parvovirus (CPV) is a major pathogen in canines, with a high mortality rate in unvaccinated puppies. CPV is traditionally classified into three antigenic variants (CPV-2a, CPV-2b and CPV-2c) based on the amino acid sequence of the VP2 protein. Currently, various mutations are described in the receptor-binding area or in the regions of greatest antigenicity of the VP2 protein, giving rise to new viral variants that are capable of immunological escape, affecting the protective immunity of traditional vaccines. In the present study, a molecular characterization of the VP2 gene was performe

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Nakamura, Masato, Kazuya Nakamura, Takayuki Miyazawa, Yukinobu Tohya, Masami Mochizuki, and Hiroomi Akashi. "Monoclonal Antibodies That Distinguish Antigenic Variants of Canine Parvovirus." Clinical Diagnostic Laboratory Immunology 10, no. 6 (2003): 1085–89. http://dx.doi.org/10.1128/cdli.10.6.1085-1089.2003.

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ABSTRACT Canine parvovirus (CPV) is classified as a member of the feline parvovirus (FPV) subgroup. CPV isolates are divided into three antigenic types: CPV type 2 (CPV-2), CPV-2a, and CPV-2b. Recently, new antigenic types of CPV were isolated from Vietnamese leopard cats and designated CPV-2c(a) or CPV-2c(b). CPV-2c viruses were distinguished from the other antigenic types of the FPV subgroup by the absence of reactivity with several monoclonal antibodies (MAbs). To characterize the antigenicity of CPV-2c, a panel of MAbs against CPV-2c was generated and epitopes recognized by these MAbs were

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Schirò, Giorgia, Francesco Mira, Marta Canuti, et al. "Identification and Molecular Characterization of a Divergent Asian-like Canine Parvovirus Type 2b (CPV-2b) Strain in Southern Italy." International Journal of Molecular Sciences 23, no. 19 (2022): 11240. http://dx.doi.org/10.3390/ijms231911240.

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Canine parvovirus type 2 (CPV-2) is an infectious agent relevant to domestic and wild carnivorans. Recent studies documented the introduction and spread of CPV-2c strains of Asian origin in the Italian canine population. We investigated tissue samples from a puppy collected during necropsy for the presence of viral enteropathogens and all samples tested positive only for CPV-2. The full coding sequence of a CPV-2b (VP2 426Asp) strain was obtained. This virus was related to CPV-2c strains of Asian origin and unrelated to European CPV-2b strains. The sequence had genetic signatures typical of As

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Tumenjargal, Sh, Ts Ariunaa, L. Ganbayar, G. Otgontuya, B. Chimedtseren, and Ch Tungalag. "Predominance of canine parvovirus type 2b in dogs of Ulaanbaatar City." Mongolian Journal of Agricultural Sciences 15, no. 2 (2015): 71–74. http://dx.doi.org/10.5564/mjas.v15i2.549.

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Canine parvovirus is a highly contagious virus that causes fatal disease acute hemorrhagic enteritis and myocarditis in dogs. The aim of this work is to detect canine parvovirus 2 (CPV-2) by standard polymerase chain reaction (PCR). Viral DNA was isolated from faecel samples of 36 puppies with suspicious symptoms for parvovirus infection and used as template in standard PCR. 23 samples wereof CPV-2b serotype, 9 samples of CPV-2a serotype but 4 samples were neither 2b and nor 2a. We used two different primer sets, one specific both serotypes CPV-2a and CPV-2b and one specific only for CPV-2b. T

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SINGH, DEEPTI, AMIT KUMAR VERMA, AMIT KUMAR, and MUKESH KUMAR SRIVASTAVA. "Molecular typing of canine parvovirus with PCR assay based on genetic markers of the antigenic variants." Indian Journal of Animal Sciences 85, no. 1 (2015): 12–15. http://dx.doi.org/10.56093/ijans.v85i1.46118.

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The canine parvovirus (CPV) is prevalent in India but only scanty literature is available on molecular differentiation of its strain using polymerase chain reaction. In the present study, 100 faecal samples from dogs suspected for canine parvo virus infection (gastroenteritis) were collected and processed for isolation of viral genomic DNA. Molecular test like PCR assay using 3 specific pairs of primers (pCPV-RT, pCPV-2ab and pCPV- 2b) was conducted for differential diagnosis of the virus type. Out of 100 faecal samples, 63 were positive for presence of CPV, of which 54 were CPV-2b and 9 were

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Pavana Jyoth, Vanjavaka, Mohana Subramanian Bhaskaran, and Vijay A. K. B. Gundi. "Epidemiology, molecular prevalence and prevention on canine parvovirus in India: A review." Bioinformation 20, no. 5 (2024): 536–46. http://dx.doi.org/10.6026/973206300200536.

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Canine parvovirus (CPV) is a highly contagious and lethal virus that causes severe gastroenteritis and myocarditis in young dogs. In 1978, CPV has rapidly spread worldwide, resulting in outbreaks and high morbidity rates among dog populations. Over a decade, CPV has undergone genetic changes, leading to the emergence of different genotypes (CPV-2a, CPV-2b, and CPV-2c), which have expanded its host range to include cats and tissue culture cells. This review focuses on CPV-2 outbreaks in India from 2010 to 2023, analyzing gene lengths covering 274-438 amino acids in the VP2 gene which are collec

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Uronu, C. E., K. Said, A. P. Muhairwa, and A. A. Chengula. "Molecular detection and characterization of canine parvovirus variants in Morogoro and Arusha regions of Tanzania." Tanzania Veterinary Journal 39, no. 1 (2024): 1–12. https://doi.org/10.4314/tvj.v39i1.1.

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Canine parvovirus (CPV) is a significant pathogen that affects wild and domestic dogs globally, resulting in intricate pathological and epidemiological consequences, primarily observed in puppies, such as haemorrhagic enteritis and myocarditis. This virus is categorized into three strains: CPV-2a (which replaced the original strain CPV-2), CPV-2b, and CPV-2c. The objective of this study was to determine the status of CPV-2 and its circulating variants in domestic dogs residing in the Morogoro and Arusha regions of Tanzania. Rectal swabs were collected from a total of 143 domestic dogs with gas

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Sharma, Kishan Kumar, Irsadullakhan Habibullakhan Kalyani, Shailee Manishbhai Pandya, and Jignesh Alabhai Vala. "Diagnosis and characterization of canine parvovirus-2 affecting canines of South Gujarat, India." Acta Veterinaria Brno 87, no. 3 (2018): 247–54. http://dx.doi.org/10.2754/avb201887030247.

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The present study was carried out in the region of South Gujarat, India, to determine the prevalence and predisposing factors of canine parvovirus-2 (CPV-2) infection in acute gastroenteritis of pups. Further, haemagglutination (HA) test, enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and rapid immunochromatography test were compared for diagnosis and prevalent CPV-2 types were ascertained. A total of 73 diarrhoea samples were collected and out of those 32, 33 and 35 were found positive via HA, ELISA and PCR, respectively. In rapid test, 26/52 samples were found pos

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Sung, Ming-Hua, Tsung-Ming Chen, Yi-Chen Yang, et al. "MOLECULAR EPIDEMIOLOGY OFCANINE PARVOVIRUS TYPE2A AND 2B AMONG SHELTER DOGS — THE NEW RECOMBINATION IN VIRUS." Taiwan Veterinary Journal 43, no. 01 (2017): 47–53. http://dx.doi.org/10.1142/s1682648515500341.

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Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen mainly affecting dogs. CPV-2 contains three antigenic variants (2a, 2b, and 2c) that are distributed internationally. Detection and characterization of the currently circulating CPV-2 strains are vital for the understanding of viral evolution, transmission, and the development of methods to control its spreading. Herein, we analyzed the strains in central Taiwan to provide information of local viral evolution, diversity, and epidemiology. Stool and blood samples from 24 dogs and 2 cats were genotyped by PCR amplification of strain-s

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Pereira, Nicole Assis, Telma Alves Monez, César Augusto Dinóla Pereira, Leonardo José Richtzenhain, and Edison Luiz Durigon. "Caracterização antigênica de isolados de parvovirus canino do Brasil utilizando monoclonais específicos." Brazilian Journal of Veterinary Research and Animal Science 45, no. 5 (2008): 385. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2008.26680.

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O Parvovírus Canino (CPV) é um patógeno emergente em cães, isolado pela primeira vez em 1978, nos Estados Unidos. A amostra original de 1978 foi designada CPV tipo 2 (CPV-2). Entretanto, análises de isolados de CPV dos Estados Unidos, por enzimas de restrição e anticorpos monoclonais demonstraram que cerca de 1979, uma amostra variante, designada CPV tipo 2a (CPV-2a) tornou-se prevalente. Subseqüentemente, uma nova amostra antigênica, designada CPV tipo 2b (CPV-2b) também foi observada por análises de isolados de CPV de várias partes do mundo, embora a proporção fosse diferente entre os países

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14

Giraldo-Ramirez, Sebastián, Santiago Rendon-Marin, and Julián Ruiz-Saenz. "Phylogenetic, Evolutionary and Structural Analysis of Canine Parvovirus (CPV-2) Antigenic Variants Circulating in Colombia." Viruses 12, no. 5 (2020): 500. http://dx.doi.org/10.3390/v12050500.

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Canine parvovirus (CPV-2) is the causative agent of haemorrhagic gastroenteritis in canids. Three antigenic variants—CPV-2a, CPV-2b and CPV-2c—have been described, which are determined by variations at residue 426 of the VP2 capsid protein. In Colombia, the CPV-2a and CPV-2b antigenic variants have previously been reported through partial VP2 sequencing. Mutations at residues Asn428Asp and Ala514Ser of variant CPV-2a were detected, implying the appearance of a possible new CPV-2a variant in Colombia. The purpose of the present study was to characterise the full VP2 capsid protein in samples fr

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Monteiro, Kyssia, Susan D. Allendorf, Acácia F. Vicente, et al. "Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil." Pesquisa Veterinária Brasileira 36, no. 12 (2016): 1181–85. http://dx.doi.org/10.1590/s0100-736x2016001200007.

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ABSTRACT: Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70’s new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central-West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All

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Loor-Giler, Anthony, Silvana Santander-Parra, Sara Castillo-Reyes, et al. "Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022–2023." Veterinary Sciences 12, no. 1 (2025): 46. https://doi.org/10.3390/vetsci12010046.

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Canine parvovirus (CPV-2) is a highly contagious virus in canines, and it is mostly spread by touching infected feces. Dogs of all ages can catch it, but puppies are more likely to suffer from it. Severe signs include vomiting, diarrhea with blood, feeling tired, and not drinking enough water. There are three different types of the original CPV-2 that have been found so far, which are CPV-2a, 2b, and 2c. The genome of CPV-2 is about 5.2 kb long and has two open reading frames (ORFs), namely the VP region and the NS region. Based on changes in amino acids at position 426, the VP2 protein distin

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Jantafong, Tippawan, Sakchai Ruenphet, Harold R. Garner, and Krit Ritthipichai. "Tracing the Genetic Evolution of Canine Parvovirus Type 2 (CPV-2) in Thailand." Pathogens 11, no. 12 (2022): 1460. http://dx.doi.org/10.3390/pathogens11121460.

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Canine parvovirus type 2 (CPV-2) is responsible for hemorrhagic gastroenteritis in dogs worldwide. High genomic substitution rates in CPV-2 contribute to the progressive emergence of novel variants with increased ability to evade the host immune response. Three studies have analyzed the genomic mutations of CPV-2 variants in Thailand. These investigations were independently conducted at different timepoints. Thus, a retrospective integrated analysis of CPV-2 genomic mutations has not been fully performed. Our study aimed at evaluating the evolutionary changes in CPV-2 in Thailand from 2003 to

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Wilson, Stephen, Joanna Illambas, Elisabeth Siedek, et al. "Vaccination of dogs with canine parvovirus type 2b (CPV-2b) induces neutralising antibody responses to CPV-2a and CPV-2c." Vaccine 32, no. 42 (2014): 5420–24. http://dx.doi.org/10.1016/j.vaccine.2014.07.102.

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WÓJCIK, ALICJA, JERZY ZIĘTEK, MARTA STANIEC, and STANISŁAW WINIARCZYK. "Genetic variability among canine parvovirus strains currently circulating in Poland." Medycyna Weterynaryjna 77, no. 05 (2021): 6525–2021. http://dx.doi.org/10.21521/mw.6525.

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The aim of the study was to perform a molecular analysis of the CPV VP2 gene fragment isolated from faeces samples of 46 dogs (vaccinated, as well as non-vaccinated against parvovirosis) with clinical parvovirosis. In all animals, the infection was confirmed by PCR. The purified PCR products (fragment VP2 gene) were sequenced and compared to each other and to the sequences of CPV VP2 gene from NCBI GenBank. Readable sequences of the VP2 gene were obtained for 46 samples. In 23 out of 30 non-vaccinated dogs, the most frequently isolated CPV strain was 2b. In the remaining cases the dogs were in

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Hyeon, Lee-Sang, Dong-Kun Yang, Eun-Ju Kim, Yu-Ri Park, Hye Jeong Lee, and Bang-Hun Hyun. "Evaluation of commercial immunochromatography test kits for diagnosing canine parvovirus." Korean Journal of Veterinary Research 63, no. 2 (2023): e19. http://dx.doi.org/10.14405/kjvr.20230016.

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Rapid immunochromatography test (RICT) kits are commonly used for the diagnosis of canine parvovirus (CPV) because of their rapid turnaround time, simplicity, and ease of use. However, the potential for cross-reactivity and low sensitivity can yield false-positive or false-negative results. There are 4 genotypes of CPV. Therefore, evaluating the performance and reliability of RICT kits for CPV detection is essential to ensure accurate diagnosis for appropriate treatment. In this study, we evaluated the performance of commercial RICT kits in the diagnosis of all CPV genotypes. The cross-reactiv

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Zienius, D., R. Lelešius, H. Kavaliauskis, A. Stankevičius, and A. Šalomskas. "Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples." Polish Journal of Veterinary Sciences 19, no. 1 (2016): 187–96. http://dx.doi.org/10.1515/pjvs-2016-0023.

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Abstract The aim of the present study was to detect canine parvovirus (CPV) from faecal samples of clinically ill domestic dogs by polymerase chain reaction (PCR) followed by VP2 gene partial sequencing and molecular characterization of circulating strains in Lithuania. Eleven clinically and antigen-tested positive dog faecal samples, collected during the period of 2014-2015, were investigated by using PCR. The phylogenetic investigations indicated that the Lithuanian CPV VP2 partial sequences (3025-3706 cds) were closely related and showed 99.0-99.9% identity. All Lithuanian sequences were as

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Hong, Charles, Nicola Decaro, Costantina Desario, et al. "Occurrence of Canine Parvovirus Type 2c in the United States." Journal of Veterinary Diagnostic Investigation 19, no. 5 (2007): 535–39. http://dx.doi.org/10.1177/104063870701900512.

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Canine parvovirus (CPV) type 2 (CPV-2) emerged around 1978 as a major pathogen of dogs worldwide. In the mid-1980s, the original CPV-2 had evolved and was completely replaced by 2 variants, CPV-2a and CPV-2b. In 2000, a new variant of CPV (named CPV-2c) was detected in Italy and now cocirculates with types 2a and 2b in that country. The CPV-2c has also been reported from single outbreaks in Vietnam and Spain. This study was conducted to determine if CPV-2c occurs in the United States. Thirty-three fecal samples were collected from dogs in 16 states between April 2006 and April 2007 and were te

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Maganga, Gael Darren, Ingrid Labouba, Serda Zita Milendz Ikapi, et al. "Molecular Characterization of Canine Parvovirus Variants CPV-2a and CPV-2c, Associated with Vaccinated Dogs at Libreville, Gabon." Viruses 15, no. 5 (2023): 1169. http://dx.doi.org/10.3390/v15051169.

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The first detection of canine parvovirus type-2 (CPV-2) was in the early 1970s, when it was known to cause severe gastroenteritis in dogs. However, it has evolved over the years into CPV-2a within 2 years, into CPV-2b after 14 years, into CPV-2c after 16 years and more recently CPV-2a-, 2b- and 2c-like variants reported in 2019, with a global distribution. Reports on the molecular epidemiology of this virus are missing in most African countries. The report of clinical cases among vaccinated dogs in Libreville in Gabon triggered the execution of this study. The objective of this study was to ch

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Watanabe, Tatiane Terumi Negrão, Edward J. Dubovi, Dawn E. Evans, et al. "Outbreak of canine parvovirus 2b and Clostridium difficile infection in Asian small-clawed otters." Journal of Veterinary Diagnostic Investigation 32, no. 2 (2019): 226–29. http://dx.doi.org/10.1177/1040638719876303.

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A concurrent outbreak of infection by canine parvovirus 2b (CPV-2b) and Clostridium difficile producing A and/or B toxins occurred in Asian small-clawed otters ( Amblonyx cinereus). The 5 clinically affected otters were 6- to 24-mo-old intact females that had severe diarrhea, dehydration, were acutely comatose, and died 1–4 d after the onset of clinical signs. Postmortem examination was performed in 3 of 7 otters. Macroscopically, the small intestine was diffusely reddened and contained red-to-brown, malodorous, watery digesta without formed feces (3 of 3). Histologic examination identified lo

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Palermo, Laura M., Susan L. Hafenstein, and Colin R. Parrish. "Purified Feline and Canine Transferrin Receptors Reveal Complex Interactions with the Capsids of Canine and Feline Parvoviruses That Correspond to Their Host Ranges." Journal of Virology 80, no. 17 (2006): 8482–92. http://dx.doi.org/10.1128/jvi.00683-06.

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ABSTRACT The cell infection processes and host ranges of canine parvovirus (CPV) and feline panleukopenia virus (FPV) are controlled by their capsid interactions with the transferrin receptors (TfR) on their host cells. Here, we expressed the ectodomains of wild-type and mutant TfR and tested those for binding to purified viral capsids and showed that different naturally variant strains of the viruses were associated with variant interactions with the receptors which likely reflect the optimization of the viral infection processes in the different hosts. While all viruses bound the feline TfR,

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Kowalczyk, Marek, Barbara Majer-Dziedzic, Krzysztof Kostro, et al. "Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland." Acta Veterinaria 69, no. 1 (2019): 32–46. http://dx.doi.org/10.2478/acve-2019-0002.

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Abstract Canine parvovirus type 2 is one of the most common causes of death among puppies. Despite preventive vaccination, the disease continues to be diagnosed. The aim of the study was to provide a molecular characterization of CPV-2 isolates found in southeastern Poland. Genetic CPV-2 material was isolated from the blood (n=10) and feces (n=50) of infected dogs. The presence of CPV-2 was confirmed by amplification of sequences coding both VP1 and VP2 protein. The products of the PCR reaction with primers amplifying VP2 protein were sequenced and used for genotyping. Bioinformatics analysis

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Magouz, AF, I. Elkon, E. Khaled, and N. Alkhalefa. "First report of detection of Canine Parvovirus type 2 in naturally infected domestic cats in Egypt by duplex PCR for simultaneous detection of Canine Parvovirus type 2 and Feline Panleukopenia virus." Journal of the Hellenic Veterinary Medical Society 74, no. 3 (2023): 5921–28. http://dx.doi.org/10.12681/jhvms.28838.

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Feline Panleukopenia virus (FPLV) and Canine Parvovirus type 2 (CPV) cause fatal gastroenteritis in cats and dogs. In this study we developed a duplex polymerase chain reaction (dPCR) assay for the concurrent detection of FPLV and CPV-2 in a single PCR tube. Two primers were used based on nucleic acid conserved regions of the two viruses which specifically amplify 237 bp of the VP2 gene of FPLV and 583 bp of the VP2 gene of CPV 2.Sensitivity and Specificity of the dPCR were evaluated. A total of 30 rectal/fecal swabs were collected from domestic cats in Kafrelsheikh province, Egypt and were te

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Steinel, A., L. Munson, M. van Vuuren, and U. Truyen. "Genetic characterization of feline parvovirus sequences from various carnivores." Microbiology 81, no. 2 (2000): 345–50. http://dx.doi.org/10.1099/0022-1317-81-2-345.

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Infections with viruses of the feline parvovirus subgroup such as feline panleukopenia virus (FPV), mink enteritis virus (MEV) and canine parvovirus (CPV-2) [together with its new antigenic types (CPV-2a, CPV-2b)] have been reported from several wild carnivore species. To examine the susceptibility of different species to the various parvoviruses and their antigenic types, samples from wild carnivores with acute parvovirus infections were collected. Viral DNA was amplified, and subsequently analysed, from faeces or formalin-fixed small intestines from an orphaned bat-eared fox (Otocyon megalot

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AKKUTAY-YOLDAR, ZEYNEP, and TAYLAN KOÇ B. "Phylogenetic analysis of canine parvoviruses from Turkey." Medycyna Weterynaryjna 76, no. 01 (2020): 6334–2020. http://dx.doi.org/10.21521/mw.6334.

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Canine parvovirus (CPV) type 2 is the causative agent of acute hemorrhagic enteritis and high mortality in the affected dogs. Numerous studies have been done to understand the origin of the virus and to exhibit new variants and circulating strains. This report describes the detection and genomic characterization of CPV strains from indoor and outdoor dogs in Ankara, Turkey. Samples were sent to our laboratory due to clinical symptoms in puppies. We tested blood and swab samples to determine the presence of canine parvovirus (CPV) in three puppies and two adult dogs by reverse transcription-pol

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Bergmann, Michèle, Monika Freisl, and Katrin Hartmann. "Prophylaxe der kaninen Parvovirose." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 49, no. 02 (2021): 122–25. http://dx.doi.org/10.1055/a-1402-9476.

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ZusammenfassungDie Impfung ist die wichtigste Maßnahme zum Schutz vor kaniner Parvovirose. Daher zählt die Impfung gegen das kanine Parvovirus (CPV) zu den Core-Komponenten der Hundeimpfung. Modifizierte Lebendvakzinen (MLV) haben sich als sehr wirksam und zugleich sicher erwiesen. Sie bieten einen zuverlässigen Schutz, der bereits nach wenigen Tagen eintritt und viele Jahre anhält. MLV enthalten keine Adjuvanzien; sie sind hinsichtlich ihres allergenen und toxischen Potenzials Impfstoffen mit inaktiviertem CPV in aller Regel überlegen. Aus diesen Gründen werden MLV Impfstoffen aus inaktiviert

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Kour, Karman, Gurpreet Kaur, Mudit Chandra, and Deepti Narang. "Development and Standardization of Sandwich ELISA for the Detection of Canine Parvovirus Infection." Journal of Biotechnology Research, no. 102 (February 10, 2024): 20–25. http://dx.doi.org/10.32861/jbr.102.20.25.

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Canine parvovirus (CPV) is a highly contagious viral pathogen affecting dogs aged 6-21 weeks primarily targeting rapidly dividing cells of GI tract, which leads to haemorrhagic gastroenteritis and symptoms such as fever, vomiting, in appetence and abdominal pain. Several variants include CPV-2, CPV-2b and CPV-2c. CPV can be prevented by timely and complete vaccination and can be treated effectively if timely diagnosis is done. It can be diagnosed by effective methods such as PCR, fecal ELISA, HA and HI, the most effective being ELISA and PCR. Therefore, the present research was aimed at develo

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Buonavoglia, Canio, Vito Martella, Annamaria Pratelli, et al. "Evidence for evolution of canine parvovirus type 2 in Italy." Journal of General Virology 82, no. 12 (2001): 3021–25. http://dx.doi.org/10.1099/0022-1317-82-12-3021.

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Two isolates of canine parvovirus (CPV) were obtained from dogs affected with severe haemorrhagic diarrhoea. Type 2b antigenic specificity was predicted by both antigenic analysis with monoclonal antibodies and PCR characterization with type-specific primers. Nevertheless, sequence analysis of the capsid protein-encoding gene revealed two amino acid changes. One of the changes affected position 426 (Asp to Glu), in a major antigenic site of the viral capsid, determining the replacement of a residue unique to CPV type 2b. The failure of established typing methods to distinguish this antigenic v

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Hao, Xiangqi, Yanchao Li, Xiangyu Xiao, Bo Chen, Pei Zhou, and Shoujun Li. "The Changes in Canine Parvovirus Variants over the Years." International Journal of Molecular Sciences 23, no. 19 (2022): 11540. http://dx.doi.org/10.3390/ijms231911540.

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Canine parvovirus (CPV-2) is one of the most important pathogens in dogs, and despite the continual development of vaccines against CPV-2, CPV-2 is still circulating in the canine population. The CPV-2a/2b/2c variant has replaced the original CPV-2 virus and seems to exhibit accelerated transmission. Although CPV-2 infection has been frequently reported, no studies have summarized information of CPV-2 variants currently circulating worldwide. To track the evolution of CPV-2, we downloaded and analyzed all VP2 sequences from the NCBI database (from 1978 to 2022). We found that CPV-2c shows a te

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Gogone, Izabel Carolina Vargas Pinto, Flavia Regina Oliveira de Barros, Flavia Possatti, Amauri Alcindo Alfieri, and Elisabete Takiuchi. "Detection of canine parvovirus types 2b and 2c in canine faecal samples contaminating urban thoroughfares in Brazil." Canadian Journal of Microbiology 66, no. 2 (2020): 138–43. http://dx.doi.org/10.1139/cjm-2019-0137.

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Canine parvovirus type 2 (CPV-2) is a highly contagious virus that causes acute gastroenteritis in dogs all over the world. Because of its stability in the environment, CPV-2 can remain infective for a long time, especially if protected in organic matter. To demonstrate CPV-2’s potential as an environmental hazard for nonimmunized susceptible hosts, we investigated 50 faecal samples collected from public areas in a municipality of Paraná state, Brazil. Seven samples tested positive for CPV by a PCR assay targeting the partial VP2 gene, with three strains being confirmed as CPV-2b variant and o

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Clark, Nicholas J., Jennifer M. Seddon, Myat Kyaw-Tanner, et al. "Emergence of canine parvovirus subtype 2b (CPV-2b) infections in Australian dogs." Infection, Genetics and Evolution 58 (March 2018): 50–55. http://dx.doi.org/10.1016/j.meegid.2017.12.013.

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Giuseppa, Purpari, Mira Francesco, Di Bella Santina, Di Pietro Simona, Giudice Elisabetta, and Guercio Annalisa. "Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay." Acta Veterinaria 68, no. 1 (2018): 80–94. http://dx.doi.org/10.2478/acve-2018-0007.

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Abstract Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and thes

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Nandi, S., S. Chidri, and M. Kumar. "Molecular characterization and phylogenetic analysis of a canine parvovirus isolate in India." Veterinární Medicína 54, No. 10 (2009): 483–90. http://dx.doi.org/10.17221/147/2009-vetmed.

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Canine parvovirus 2 (CPV-2) is the causative agent of acute hemorrhagic enteritis and myocarditis in dogs. In this study the nucleotide sequence of the VP1/VP2 gene of a CPV isolate from India was analyzed and the phylogenetic relationship with other CPV isolates was established. Out of 36 samples analyzed, 16 were found positive for CPV-2 by polymerase chain reaction (PCR). Among the 16 positive samples, five were inoculated in MDCK cells for isolation out of which one adapted successfully to the cell culture system. Phylogenetic analysis based on the nucleotide sequence of the VP-1/VP-2 gene

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Jeoung, Seok-Young. "Evaluation for Protective Effect of CPV-2 and CPV-2b Vaccines against a Korean CPV-2a Isolate in Pups." Journal of Veterinary Clinics 31, no. 5 (2014): 361. http://dx.doi.org/10.17555/ksvc.2014.10.31.5.361.

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Zaher, Kawther Sayed, Wahid Hussein El-Dabae, Mostafa Mohamed El-Sebelgy, Naglaa Ibrahim Aly, and Zeinab Taha Salama. "Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt." February-2020 13, no. 2 (2020): 326–33. http://dx.doi.org/10.14202/vetworld.2020.326-333.

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Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018. Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylog

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KOUR, KARMAN, GURPREET KAUR, MUDIT CHANDRA, and P. N. DWIVEDI. "Real time PCR typing of canine parvovirus types in various regions of northern India." Indian Journal of Animal Sciences 92, no. 8 (2022): 931–34. http://dx.doi.org/10.56093/ijans.v92i8.106851.

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Canine parvovirus (CPV) is a ssDNA virus which has high morbidity in unvaccinated and some vaccinated dogs as well. It is seen to be evolving and has various antigenic types. The present study was aimed to study the prevalence of the antigenic type in northern India by real-time PCR. This study was conducted in 2019 wherein 178 samples (collected from Delhi, Punjab, Jammu, Chandigarh and Assam) available in the Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana were subjected to real time PCR for CPV 2, CPV 2

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Jinadasa, Rasika, Sayani Ghosh, Simon Hills, et al. "Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka." Pathogens 10, no. 9 (2021): 1102. http://dx.doi.org/10.3390/pathogens10091102.

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The aim of the study was to identify canine parvovirus type 2 (CPV-2) subtypes circulating among a selected population of domestic dogs and cats in Sri Lanka and to investigate the evolutionary patterns among Sri Lankan viruses in the context of contemporary global CPV-2 sequences. Altogether, 40/61 (65.6%) samples tested were positive for CPV-2 DNA, including 31/48 (64.6%) dogs and 9/13 (69%) cats. All three subtypes (CPV-2a, CPV-2b and CPV-2c) were detected, with CPV-2a being most common. International median joining haplotype network of 291 CPV-2 sequences suggested that there was little ba

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Mira, Francesco, Giovanni Franzo, Giorgia Schirò, et al. "Introduction of a Divergent Canine Parvovirus Type 2b Strain with a Dog in Sicily, Southern Italy, Through the Mediterranean Sea Route to Europe." Pathogens 14, no. 2 (2025): 108. https://doi.org/10.3390/pathogens14020108.

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Despite over four decades since its emergence, canine parvovirus type 2 (CPV-2) remains a relevant disease for dogs. Few studies, primarily only recent ones based on phylodynamic and phylogeography approaches, have highlighted the impact of rapid and long-distance transport of dogs on the CPV-2 spreading dynamics. The present study reports the genomic characterization of a CPV-2 strain detected in a dog introduced into Italy from the coasts of North Africa through the Mediterranean Sea route to Europe. The nearly complete CPV-2 sequence was obtained and analyzed. The viral isolate was characte

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Pan, Shunshun, Yuanzhuo Man, Xin Xu, et al. "Genetic Diversity and Recombination Analysis of Canine Parvoviruses Prevalent in Central and Eastern China, from 2020 to 2023." Microorganisms 12, no. 11 (2024): 2173. http://dx.doi.org/10.3390/microorganisms12112173.

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Canine parvovirus type-2 (CPV-2), the primary causative agent of serious canine enteric diseases, is highly contagious and associated with high fatality rates worldwide. To comprehend the current emergence of CPV-2 in central and eastern China, 130 rectal swabs from domestic or stray dogs with gastroenteritis symptoms were collected during 2020–2023. A total of 118 positive samples were detected via polymerase chain reaction, and further used to amplify and sequence the VP2 gene. Sequence analysis of the deduced amino acids of VP2 protein indicated that CPV-2c was the most prevalent variant (n

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Gainor, Kerry, April Bowen, Pompei Bolfa, Andrea Peda, Yashpal S. Malik, and Souvik Ghosh. "Molecular Investigation of Canine Parvovirus-2 (CPV-2) Outbreak in Nevis Island: Analysis of the Nearly Complete Genomes of CPV-2 Strains from the Caribbean Region." Viruses 13, no. 6 (2021): 1083. http://dx.doi.org/10.3390/v13061083.

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To date, there is a dearth of information on canine parvovirus-2 (CPV-2) from the Caribbean region. During August–October 2020, the veterinary clinic on the Caribbean island of Nevis reported 64 household dogs with CPV-2-like clinical signs (hemorrhagic/non-hemorrhagic diarrhea and vomiting), of which 27 animals died. Rectal swabs/fecal samples were obtained from 43 dogs. A total of 39 of the 43 dogs tested positive for CPV-2 antigen and/or DNA, while 4 samples, negative for CPV-2 antigen, were not available for PCR. Among the 21 untested dogs, 15 had CPV-2 positive littermates. Analysis of th

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Khadse, Monali Bhagwan, Shubhangi Rambhau, and Sujit Kolangath. "Molecular Detection and Phylogenetic Analysis of Canine Parvovirus-2 in Dogs." Indian Journal of Veterinary Sciences & Biotechnology 19, no. 2 (2023): 54–57. http://dx.doi.org/10.48165/ijvsbt.19.2.10.

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Canine Parvovirus (CPV) is a most significant viral disease causing acute haemorrhagic gastroenteritis and myocarditis in puppies at the age of 3-4 months. It causes serious morbidity and mortality, in spite of the effective vaccination. Inadequate immunity and modern practices of early weaning in the kennels have predisposed the young dogs to CPV. In the present study, total 50 fecal samples were collected from dogs suspected for CPV-2 from Nagpur, India and screened by Ubioquick® VET lateral flow assay kit and Polymerase Chain Reaction (PCR) followed by the sequencing and phylogenetic analys

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Hu, Wen, Liangyan Zheng, Xin Xu, et al. "Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019." Journal of Veterinary Research 64, no. 4 (2020): 477–86. http://dx.doi.org/10.2478/jvetres-2020-0076.

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AbstractIntroductionCanine parvovirus type-2 (CPV-2) causes acute infectious diseases in puppies, which show high morbidity and mortality. Better effect of vaccination against these diseases could be achieved with deeper knowledge of CPV-2 genotype dissemination and mutation history. This study investigated CPV-2–positive samples collected recently over a wide region of China.Material and MethodsA total of 118 faecal samples from dogs identified as CPV-positive were collected from veterinary clinics in central and eastern China. Overall, 16 strains collected from Anhui, 29 from Henan, and 16 f

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Lange, Jeannette, Marco Groth, Dieter Fichtner, et al. "Virus isolate from carp: genetic characterization reveals a novel picornavirus with two aphthovirus 2A-like sequences." Journal of General Virology 95, no. 1 (2014): 80–90. http://dx.doi.org/10.1099/vir.0.058172-0.

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Picornaviruses have been isolated from a variety of hosts, mainly mammals and birds. Here, we describe the sequence analysis of carp picornavirus 1 (CPV-1) F37/06 that was isolated from an organ pool (heart, brain, liver) of a common carp (Cyprinus carpio). This carp perished after an accidental discharge of liquid manure into a fish pond and presented without obvious clinical symptoms. Experimental intraperitoneal infection of young carp with CPV-1 revealed no clinical signs, but the virus was re-isolated from various organs. Sequence analysis of almost the complete genome (7632 nt excluding

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Shivani, Barsain Subhash Verma Rajesh Chahota Prasenjit Dhar and Monika Bhardwaj. "Key strains of canine parvovirus 2 in india: a short communication." Vet Farm Frontier 02, no. 04 (2025): 19–22. https://doi.org/10.5281/zenodo.15362298.

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Canine Parvovirus (CPV) is a highly infectious viral illness that primarily affects dogs, especially puppies aged between six weeks and six months. It is caused by Canine Parvovirus type 2 (CPV-2), which mainly targets the gastrointestinal system and, in some instances, the heart. The virus attacks rapidly dividing cells such as those in the intestinal crypts, bone marrow, and lymphoid tissues, resulting in enteritis, leukopenia, and immunosuppression. Since its discovery, CPV-2 has evolved into multiple strains, contributing to its high virulence. This article focuses on the predominant CPV-2

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WÓJCIK, ALICJA, STANISŁAW WINIARCZYK, and JERZY ZIĘTEK. "Canine parvovirosis: Still an existing problem." Medycyna Weterynaryjna 75, no. 04 (2019): 6191–2019. http://dx.doi.org/10.21521/mw.6191.

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Parvoviral infections of dogs are still a clinical problem throughout the world. This is despite the development of prevention, increased awareness among the owners and increasingly effective treatment protocols due to the extremely high virulence of the virus. Parvovirosis is a disease caused by a virus of the Parvoviridae family in various variants: CPV-2, CPV-2a, CPV-2b and CPV-2c, all of which produce similar clinical signs, including acute hemorrhagic enteritis and myocarditis. It is one of the most important viral pathogens, with extremely high morbidity and mortality. This study is a co

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Temizkan, Mehmet Cevat, and Secil Sevinc Temizkan. "Canine Parvovirus in Turkey: First Whole-Genome Sequences, Strain Distribution, and Prevalence." Viruses 15, no. 4 (2023): 957. http://dx.doi.org/10.3390/v15040957.

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Canine parvovirus (CPV) is a significant pathogenic virus with up to 100% morbidity and 91% mortality rates, especially in unvaccinated puppies. The emergence of new strains, interspecies transmission, and vaccine effectiveness can be enabled by just a few base changes in the CPV genome. Therefore, to cope with CPV disease, it is important to identify the viral agent and regularly monitor vaccine effectiveness against new strains. The present study has investigated CPV’s genetic profile in Turkey by collecting 80 samples from dogs in Turkey between 2020 and 2022. These samples and all sequence

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