Academic literature on the topic 'Cre Lox'

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Journal articles on the topic "Cre Lox"

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Lee, Linda, and Paul D. Sadowski. "Lox and Cre sandwich." Nature Chemical Biology 1, no. 5 (2005): 246–47. http://dx.doi.org/10.1038/nchembio1005-246.

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Becher, B., A. Waisman, and L. F. Lu. "Cre-lox: Target Sensitivity Matters." Immunity 51, no. 4 (2019): 595. http://dx.doi.org/10.1016/j.immuni.2019.09.012.

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Gewin, Leslie S. "The Cre/lox system: Cre-ating unintended damage." American Journal of Physiology-Renal Physiology 316, no. 5 (2019): F873—F874. http://dx.doi.org/10.1152/ajprenal.00428.2018.

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Gilbertson, Larry. "Cre–lox recombination: Cre-ative tools for plant biotechnology." Trends in Biotechnology 21, no. 12 (2003): 550–55. http://dx.doi.org/10.1016/j.tibtech.2003.09.011.

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Testa, Giuseppe, and A. Francis Stewart. "Cre ating a trans lox ation." EMBO reports 1, no. 2 (2000): 120–21. http://dx.doi.org/10.1093/embo-reports/kvd035.

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Cui, Yunxia, Lu Huang, Florent Elefteriou, et al. "Essential Role of STAT3 in Body Weight and Glucose Homeostasis." Molecular and Cellular Biology 24, no. 1 (2004): 258–69. http://dx.doi.org/10.1128/mcb.24.1.258-269.2004.

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ABSTRACT STAT3 is a ubiquitous transcription factor that is indispensable during early embryogenesis. To study the functions of STAT3 postnatally, we generated conditional STAT3-deficient mice. To that end, STAT3lox/lox mice were crossed with mice expressing Cre under the control of rat insulin II gene promoter (RIP-Cre mice). Immunohistochemical and Western blot analyses showed that STAT3 is deleted from β cells in the islets of Langerhans. Genomic DNA PCR revealed that STAT3 deletion also occurred in the hypothalamus. Hypothalamic Cre expression was further confirmed by crossing RIP-Cre/STAT
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Parrish, Diana C., Eric N. Alston, Hermann Rohrer та ін. "Absence of gp130 in dopamine β-hydroxylase-expressing neurons leads to autonomic imbalance and increased reperfusion arrhythmias". American Journal of Physiology-Heart and Circulatory Physiology 297, № 3 (2009): H960—H967. http://dx.doi.org/10.1152/ajpheart.00409.2009.

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Inflammatory cytokines that act through glycoprotein (gp)130 are elevated in the heart after myocardial infarction and in heart failure. These cytokines are potent regulators of neurotransmitter and neuropeptide production in sympathetic neurons but are also important for the survival of cardiac myocytes after damage to the heart. To examine the effect of gp130 cytokines on cardiac nerves, we used gp130DBH-Cre/lox mice, which have a selective deletion of the gp130 cytokine receptor in neurons expressing dopamine β-hydroxylase (DBH). Basal sympathetic parameters, including norepinephrine (NE) c
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Suzuki, Nobuaki, Hiroshi Nonaka, Yota Tsuge, Masayuki Inui, and Hideaki Yukawa. "New Multiple-Deletion Method for the Corynebacterium glutamicum Genome, Using a Mutant lox Sequence." Applied and Environmental Microbiology 71, no. 12 (2005): 8472–80. http://dx.doi.org/10.1128/aem.71.12.8472-8480.2005.

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ABSTRACT Due to the difficulty of multiple deletions using the Cre/loxP system, a simple, markerless multiple-deletion method based on a Cre/mutant lox system combining a right-element (RE) mutant lox site with a left-element (LE) mutant lox site was employed for large-scale genome rearrangements in Corynebacterium glutamicum. Eight distinct genomic regions that had been identified previously by comparative analysis of C. glutamicum R and C. glutamicum 13032 genomes were targeted for deletion. By homologous recombination, LE and RE mutant lox sites were integrated at each end of a target regio
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Yan, Xin, Hao-Jie Yu, Qing Hong, and Shun-Peng Li. "Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis." Applied and Environmental Microbiology 74, no. 17 (2008): 5556–62. http://dx.doi.org/10.1128/aem.01156-08.

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ABSTRACT We have developed a fast and accurate method to engineer the Bacillus subtilis genome that involves fusing by PCR two flanking homology regions with an antibiotic resistance gene cassette bordered by two mutant lox sites (lox71 and lox66). The resulting PCR products were used directly to transform B. subtilis, and then transient Cre recombinase expression in the transformants was used to recombine lox71 and lox66 into a double-mutant lox72 site, thereby excising the marker gene. The mutation process could also be accomplished in 2 days by using a strain containing a cre isopropyl-β-d-
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Lambert, Jolanda M., Roger S. Bongers, and Michiel Kleerebezem. "Cre-lox-Based System for Multiple Gene Deletions and Selectable-Marker Removal in Lactobacillus plantarum." Applied and Environmental Microbiology 73, no. 4 (2006): 1126–35. http://dx.doi.org/10.1128/aem.01473-06.

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ABSTRACT The classic strategy to achieve gene deletion variants is based on double-crossover integration of nonreplicating vectors into the genome. In addition, recombination systems such as Cre-lox have been used extensively, mainly for eukaryotic organisms. This study presents the construction of a Cre-lox-based system for multiple gene deletions in Lactobacillus plantarum that could be adapted for use on gram-positive bacteria. First, an effective mutagenesis vector (pNZ5319) was constructed that allows direct cloning of blunt-end PCR products representing homologous recombination target re
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Dissertations / Theses on the topic "Cre Lox"

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Paul, Sunirmal. "Topological constraint of lox-cre site-specific recombination." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621634.

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Mägdefrau, Marion. "Ortsgerichtete Rekombination in Chlamydomonas reinhardtii am Beispiel des Cre/lox-Systems." kostenfrei, 2007. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/880/.

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Taveira-Marques, R. "Fate-mapping neural stem cells in the mouse ventral neural tube by Cre-lox transgenesis." Thesis, University College London (University of London), 2011. http://discovery.ucl.ac.uk/1333991/.

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Neurons and glia (astrocytes and oligodendrocytes) are the two major cell types that make up the central nervous system (CNS). They are generated from precursor domains within the neuroepithelial germinal zone (ventricular zone, VZ) that surrounds the ventricles of the brain and the central canal of the spinal cord (the embryonic neural tube). In general, neurons are generated before glia. The intra-spinal circuits that control movement and locomotion are made up of different neuronal and glial elements that develop separately but come together to form interconnected functional units. To under
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Shaw, Daniel 1993. "Streamlining minimal bacterial genomes : Analysis of the pan bacterial essential genome, and a novel strategy for random genome deletions in Mycoplasma pneumoniae." Doctoral thesis, Universitat Pompeu Fabra, 2019. http://hdl.handle.net/10803/668244.

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Understanding what constitutes a true Minimal Cell is a key challenge in synthetic biology. In this work, we present two new tools to aid in this endeavour. i) A novel methodology for minimising the Mycoplasma pneumoniae genome via random deletions of genetic material. This protocol utilises the Cre Lox system coupled with random transposon mutagenesis to create a population with random lox sites dispersed around the genome. This allows for a population of cells containing a high variability of large and small-scale deletions ranging from 50bp to 25Kb within M. pneumoniae. ii) The first large
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Kvist, A. P. (Ari-Pekka). "Type XIII collagen:organization and chromosomal localization of the mouse gene, distance between human COL13A1 and prolyl 4-hydroxylase α-subunit genes, and generation of mice expressing an N-terminally altered type XIII collagen". Doctoral thesis, Oulun yliopisto, 1999. http://urn.fi/urn:isbn:9514253949.

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Abstract The complete exon-intron organization of the gene coding for the mouse α1(XIII) collagen chain, Col13a1, was characterized from genomic clones and multiple transcription initiation points were determined. Detailed comparison of the human and mouse genes showed that the exon-intron structures are completely conserved between the species, and both genes have their 5' untranslated region preceded by a highly conserved putative promoter region. The chromosomal location of the mouse gene was determined to be at chromosome 10, band B4, between markers D10Mit5 – (2.3 ± 1.6 cM) – Col13a1 – (3
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Machado, Caroline. "STUDIES OF ERGOT ALKALOID BIOSYNTHESIS GENES IN CLAVICIPITACEOUS FUNGI." UKnowledge, 2004. http://uknowledge.uky.edu/gradschool_diss/433.

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Neotyphodium species, endophytic fungi associated with cool-season grasses, enhance host fitness and stress tolerance, but also produce biologically active alkaloids including ergot alkaloids associated with fescue toxicosis in grazing animals. One approach to reduce fescue toxicosis is to manipulate genes in the ergot alkaloid pathway. The gene, dmaW, encoding the first pathway-specific step in ergot alkaloid biosynthesis, was cloned previously from Claviceps spp. and its function was demonstrated by expression in yeast. Putative homologs have been cloned from Neotyphodium coenophialum (from
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Duran, Ortiz Silvana. "Characterization and Lifespan Assessment of Inducible Growth Hormone ReceptorDisrupted Mice at Six Months of Age." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1602089078681852.

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Cattin, Anne-Laure. "Le récepteur nucléaire HNF-4α : un facteur au carrefour entre homéostasie, architecture et fonction de l'épithélium intestinal adulte". Paris 6, 2009. http://www.theses.fr/2009PA066379.

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L’épithélium intestinal est un système en renouvellement constant. Son homéostasie repose sur la coordination précise des processus de prolifération, de différenciation et de survie cellulaire. L’objectif de mon travail de thèse a été d’étudier le rôle du facteur de transcription HNF-4α dans l’homéostasie, l’architecture et la fonction de l’épithélium intestinal. Dans un modèle de souris adultes, où l’invalidation du gène Hnf-4α est inductible et spécifique de l’intestin, j’ai montré qu’HNF-4α est un acteur clé du maintien de l’homéostasie de cet épithélium : HNF-4α module la prolifération dan
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Kalamarides, Michel. "Modélisation de la tumorigenèse méningée chez la souris récapitulant les altérations génétiques des méningiomes humains." Paris 7, 2005. http://www.theses.fr/2005PA077146.

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Rehmani, Taha. "An In-vivo Analysis of SLMAP Function in the Postnatal Mouse Myocardium." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36666.

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SLMAP is a tail anchored membrane protein that alternatively splices to generate three isoforms, SLMAP1, SLMAP2 and SLMAP3. Previous studies in our lab have shown that the postnatal cardiac-specific overexpression of SLMAP1 results in intracellular vesicle expansion and enhanced endosomal recycling. I generated a postnatal cardiac-specific knockout model using the Cre-Lox system to nullify all three SLMAP isoforms and further evaluate its role in the mouse myocardium. SLMAP knockdown and knockout mouse hearts were analyzed with western blotting and qPCR. I found that only SLMAP3 was nullified
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Books on the topic "Cre Lox"

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Primary low vision care. Appleton & Lange, 1994.

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Sánchez, Germán. Los enigmas del Che. Ediciones Ko'eyu, 1997.

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Ku lou ma che. Jie li chu ban she, 2004.

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Rushing, Felder. Tough plants for Florida gardens: Low care, no care, tried and true winners. Cool Springs Press, 2004.

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Melby, Jeffrey A. CORE-LOC concrete armor units. U.S. Army Corps of Engineers, Waterways Experiment Station, 1997.

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Melby, Jeffrey A. CORE-LOC concrete armor units. U.S. Army Engineer Waterways Experiment Station, 1997.

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Vaca, Gustavo Parada. Los compañeros del Che Guevara. s.n., 1997.

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Korol, Claudia. El Che y los argentinos. Ediciones Dialéctica, 1988.

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Melby, Jeffrey A. CORE-LOC concrete armor units. U.S. Army Corps of Engineers, Waterways Experiment Station, 1997.

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Symposium, Interdisciplinary Health Care. Interdisciplinary Health Care Symposium (1992: Portland, Ore.) Proceedings. National College of Naturopathic Medicine, 1992.

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Book chapters on the topic "Cre Lox"

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Hoess, R. H., and K. Abremski. "The Cre-lox Recombination System." In Nucleic Acids and Molecular Biology. Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-84150-7_6.

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Srivastava, Vibha, and David W. Ow. "Simplifying Transgene Locus Structure Through Cre-lox Recombination." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2453-0_6.

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Albert, Henrik, and David W. Ow. "Cre-lox Directed Integration of Transgenes into the Tobacco Genome." In Plant Molecular Biology Manual. Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4217-5_3.

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Gilbertson, Larry, Waly Dioh, Prince Addae, et al. "Cre/lox Mediated Marker Gene Excision in Transgenic Crop Plants." In Plant Biotechnology 2002 and Beyond. Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-2679-5_43.

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Smith, Lorna I. F., Thomas G. Hill, and James E. Bowe. "Generating Beta-Cell-Specific Transgenic Mice Using the Cre-Lox System." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0385-7_13.

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Mobley, Aaron K., and Joseph H. McCarty. "Use of Cre-Lox Technology to Analyze Integrin Functions in Astrocytes." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-452-0_37.

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Baker, Lorina G., and Jennifer K. Lodge. "Multiple Gene Deletion in Cryptococcus neoformans Using the Cre–lox System." In Host-Fungus Interactions. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-539-8_6.

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Kratochwil, Claudius F., and Filippo M. Rijli. "The Cre/Lox System to Assess the Development of the Mouse Brain." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-655-9_20.

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Kratochwil, Claudius F., and Filippo M. Rijli. "The Cre/Lox System to Assess the Development of the Mouse Brain." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9732-9_28.

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Dessen, Pierre, Joerg Huelsken, and Paloma Ordóñez-Morán. "Specific Gene Expression in Lgr5+ Stem Cells by Using Cre-Lox Recombination." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0747-3_16.

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Conference papers on the topic "Cre Lox"

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Subbotin, K. A., V. V. Slavkina, D. A. Lis, O. N. Lis, and E. V. Zharikov. "Evolution of Cr4+, Cr3+and Cr2+contents in Cr:Mg2SiO4 crystals during those oxidizing annealing." In 2016 International Conference Laser Optics (LO). IEEE, 2016. http://dx.doi.org/10.1109/lo.2016.7549671.

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Zhang, Sheng, Adrian Tang, Zhewei Jiang, Simha Sethumadhavan, and Mingoo Seok. "Blacklist Core." In ISLPED '18: International Symposium on Low Power Electronics and Design. ACM, 2018. http://dx.doi.org/10.1145/3218603.3218624.

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van der Heide, Sjoerd, Juan Carlos Alvarado-Zacarias, Nicolas K. Fontaine, et al. "Low-loss Low-MDL Core Multiplexer for 3-Core Coupled-core Multi-core Fiber." In Optical Fiber Communication Conference. OSA, 2020. http://dx.doi.org/10.1364/ofc.2020.t3a.3.

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Zhong, Guo-Qiang, Pei-Li Sun, and Hung-Shing Chen. "SPARKLE ESTIMATION OF METALLIC SAMPLES USING A LOW-COST SYSTEM." In CIE 2018. International Commission on Illumination, CIE, 2018. http://dx.doi.org/10.25039/x45.2018.po22.

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Sa-Ngadsup, Pracharat, Eric Dinet, Pichayada Katemake, and Alain Trémeau. "USING LIGHT TO FACILITATE THE MOBILITY OF LOW VISION PEOPLE." In CIE 2018. International Commission on Illumination, CIE, 2018. http://dx.doi.org/10.25039/x45.2018.op29.

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Karimi, Somayeh, Milad Saidian, and Hossein Kazemi. "Experimental Study of the Effect of Core Aging on Fluid Distribution in Middle Bakken Cores." In SPE Low Perm Symposium. Society of Petroleum Engineers, 2016. http://dx.doi.org/10.2118/180269-ms.

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Dugan, Edward T., William E. Lear, and Gerard E. Welch. "Pulsed Gas Core Reactor For Burst Power." In 1988 Los Angeles Symposium--O-E/LASE '88, edited by Raymond F. Askew. SPIE, 1988. http://dx.doi.org/10.1117/12.943627.

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Bussard, Robert W. "Fundamental Considerations Of Gas Core Reactor Systems." In 1988 Los Angeles Symposium--O-E/LASE '88, edited by Raymond F. Askew. SPIE, 1988. http://dx.doi.org/10.1117/12.943628.

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Qu, Yuan. "Electron mobility in GaN-based core-shell nanowires (Conference Presentation)." In Low-Dimensional Materials and Devices 2018, edited by Nobuhiko P. Kobayashi, A. Alec Talin, Albert V. Davydov, and M. Saif Islam. SPIE, 2018. http://dx.doi.org/10.1117/12.2320150.

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Bornstein, A., and L. Boehm. "Hollow And Glass Core Chalcogenide Fibers." In O-E/LASE'86 Symp (January 1986, Los Angeles), edited by Paul Klocek. SPIE, 1986. http://dx.doi.org/10.1117/12.961108.

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Reports on the topic "Cre Lox"

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Conti, Claudio J. Studies of Prostate Tumor Development via Cre/LoxP Technology. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada416737.

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COnti, Claudio J. Studies of Prostate Tumor Development via Cre/LoxP Technology. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada407387.

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Conti, Claudio J. Studies of Prostate Tumor Development via Cre/LoxP Technology. Defense Technical Information Center, 2004. http://dx.doi.org/10.21236/ada435229.

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Smith, Kristin, and Reagan Baughman. Low wages prevalent In direct care and child care workforce. University of New Hampshire Libraries, 2007. http://dx.doi.org/10.34051/p/2020.26.

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Smith, Kristin, and Nicholas Adams. Child care subsidies critical for low-income families amid rising child care expenses. University of New Hampshire Libraries, 2013. http://dx.doi.org/10.34051/p/2020.195.

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Starguist, V. L. Core log: Valles caldera No. 2A, New Mexico. Office of Scientific and Technical Information (OSTI), 1988. http://dx.doi.org/10.2172/5165076.

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Turk, George F., and Jeffrey A. Melby. CORE-LOC (trade name) Concrete Armor Units: Technical Guidelines. Defense Technical Information Center, 1997. http://dx.doi.org/10.21236/ada328538.

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D.A. Gates, C. Jun, I. Zatz, A. Zolfaghari. All Metal Iron Core For A Low Aspect Ratio Tokamak. Office of Scientific and Technical Information (OSTI), 2010. http://dx.doi.org/10.2172/981710.

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Ellis, Hoi, Michelle Dick, and Chris Kivett. Child Care and Transportation Options for Iowa City's Low-Income Residents. University of Iowa, 1995. http://dx.doi.org/10.17077/sz2w-7vo5.

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Smith, Kristin, and Kristi Gozjolko. Low income and impoverished families pay disproportionately more for child care. University of New Hampshire Libraries, 2010. http://dx.doi.org/10.34051/p/2020.93.

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