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Dissertations / Theses on the topic 'Cre Lox'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

Paul, Sunirmal. "Topological constraint of lox-cre site-specific recombination." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621634.

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2

Mägdefrau, Marion. "Ortsgerichtete Rekombination in Chlamydomonas reinhardtii am Beispiel des Cre/lox-Systems." kostenfrei, 2007. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/880/.

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3

Taveira-Marques, R. "Fate-mapping neural stem cells in the mouse ventral neural tube by Cre-lox transgenesis." Thesis, University College London (University of London), 2011. http://discovery.ucl.ac.uk/1333991/.

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Neurons and glia (astrocytes and oligodendrocytes) are the two major cell types that make up the central nervous system (CNS). They are generated from precursor domains within the neuroepithelial germinal zone (ventricular zone, VZ) that surrounds the ventricles of the brain and the central canal of the spinal cord (the embryonic neural tube). In general, neurons are generated before glia. The intra-spinal circuits that control movement and locomotion are made up of different neuronal and glial elements that develop separately but come together to form interconnected functional units. To under

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4

Shaw, Daniel 1993. "Streamlining minimal bacterial genomes : Analysis of the pan bacterial essential genome, and a novel strategy for random genome deletions in Mycoplasma pneumoniae." Doctoral thesis, Universitat Pompeu Fabra, 2019. http://hdl.handle.net/10803/668244.

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Understanding what constitutes a true Minimal Cell is a key challenge in synthetic biology. In this work, we present two new tools to aid in this endeavour. i) A novel methodology for minimising the Mycoplasma pneumoniae genome via random deletions of genetic material. This protocol utilises the Cre Lox system coupled with random transposon mutagenesis to create a population with random lox sites dispersed around the genome. This allows for a population of cells containing a high variability of large and small-scale deletions ranging from 50bp to 25Kb within M. pneumoniae. ii) The first large

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5

Kvist, A. P. (Ari-Pekka). "Type XIII collagen:organization and chromosomal localization of the mouse gene, distance between human COL13A1 and prolyl 4-hydroxylase α-subunit genes, and generation of mice expressing an N-terminally altered type XIII collagen". Doctoral thesis, Oulun yliopisto, 1999. http://urn.fi/urn:isbn:9514253949.

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Abstract The complete exon-intron organization of the gene coding for the mouse α1(XIII) collagen chain, Col13a1, was characterized from genomic clones and multiple transcription initiation points were determined. Detailed comparison of the human and mouse genes showed that the exon-intron structures are completely conserved between the species, and both genes have their 5' untranslated region preceded by a highly conserved putative promoter region. The chromosomal location of the mouse gene was determined to be at chromosome 10, band B4, between markers D10Mit5 – (2.3 ± 1.6 cM) – Col13a1 – (3

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6

Machado, Caroline. "STUDIES OF ERGOT ALKALOID BIOSYNTHESIS GENES IN CLAVICIPITACEOUS FUNGI." UKnowledge, 2004. http://uknowledge.uky.edu/gradschool_diss/433.

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Neotyphodium species, endophytic fungi associated with cool-season grasses, enhance host fitness and stress tolerance, but also produce biologically active alkaloids including ergot alkaloids associated with fescue toxicosis in grazing animals. One approach to reduce fescue toxicosis is to manipulate genes in the ergot alkaloid pathway. The gene, dmaW, encoding the first pathway-specific step in ergot alkaloid biosynthesis, was cloned previously from Claviceps spp. and its function was demonstrated by expression in yeast. Putative homologs have been cloned from Neotyphodium coenophialum (from

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7

Duran, Ortiz Silvana. "Characterization and Lifespan Assessment of Inducible Growth Hormone ReceptorDisrupted Mice at Six Months of Age." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1602089078681852.

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8

Cattin, Anne-Laure. "Le récepteur nucléaire HNF-4α : un facteur au carrefour entre homéostasie, architecture et fonction de l'épithélium intestinal adulte". Paris 6, 2009. http://www.theses.fr/2009PA066379.

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L’épithélium intestinal est un système en renouvellement constant. Son homéostasie repose sur la coordination précise des processus de prolifération, de différenciation et de survie cellulaire. L’objectif de mon travail de thèse a été d’étudier le rôle du facteur de transcription HNF-4α dans l’homéostasie, l’architecture et la fonction de l’épithélium intestinal. Dans un modèle de souris adultes, où l’invalidation du gène Hnf-4α est inductible et spécifique de l’intestin, j’ai montré qu’HNF-4α est un acteur clé du maintien de l’homéostasie de cet épithélium : HNF-4α module la prolifération dan

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9

Kalamarides, Michel. "Modélisation de la tumorigenèse méningée chez la souris récapitulant les altérations génétiques des méningiomes humains." Paris 7, 2005. http://www.theses.fr/2005PA077146.

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10

Rehmani, Taha. "An In-vivo Analysis of SLMAP Function in the Postnatal Mouse Myocardium." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36666.

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SLMAP is a tail anchored membrane protein that alternatively splices to generate three isoforms, SLMAP1, SLMAP2 and SLMAP3. Previous studies in our lab have shown that the postnatal cardiac-specific overexpression of SLMAP1 results in intracellular vesicle expansion and enhanced endosomal recycling. I generated a postnatal cardiac-specific knockout model using the Cre-Lox system to nullify all three SLMAP isoforms and further evaluate its role in the mouse myocardium. SLMAP knockdown and knockout mouse hearts were analyzed with western blotting and qPCR. I found that only SLMAP3 was nullified

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11

Isaksson, Amanda. "Optimization of PCR protocols used for genotyping transgenic mice & Evaluation of a method for co-detecting mRNA and protein." Thesis, Uppsala universitet, Jämförande fysiologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-326540.

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The aim of the current study was divided into two separate goals, (i) optimization of a number of PCR-based protocols employed for genotyping transgenic mouse lines and (ii) evaluating a protocol for co-detection of mRNA and its correlated protein in the mouse midbrain. The optimization was performed on PCR protocols for genotyping the following transgenic mouse lines; Dat-Cre, Vglut2-Lox, Vglut2-Cre and Vmat2-Lox. Also, two different polymerases were evaluated parallel to each other – KAPA and Maxima Hot Start. One of the main findings from the PCR optimizations were that for the Vglut2-Lox p

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12

Roeske, Cassandra. "Role of the Heterotrimeric Go Protein Alpha-subunit on the Cardiac Secretory Phenotype." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/24191.

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Atrial natriuretic factor (ANF) is a polypeptide hormone produced in heart atria, stored in atrial secretory granules and released into the circulation in response to various stimuli. Proper sorting of ANF at the level of the trans-Golgi network (TGN) is required for the storage of ANF in these specific granules, and this sorting of hormones has been found to be associated with G-proteins. Specifically, the Go protein alpha-subunit (Gαo) was established to participate in the stretch-secretion coupling of ANF, but may also be involved in the transporting of ANF from the TGN into atrial granul

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13

Carey, Jayne Victoria. "Assessing the Cre/loxp recombination in Plasmodium berghei." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271522.

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14

Jung, Josephine [Verfasser]. "Analyse der Immunantwort gegen Kolontumoren im Villin-Cre-ERT2 x LoxP-Tag- und Lgr5-Cre-ERT2 x LoxP-Tag-Mausmodell / Josephine Jung." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2017. http://d-nb.info/1148425543/34.

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15

Merki, Esther. "Tissue restricted disruption of retinoid signaling using Cre-LoxP /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3044792.

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16

Eriksen, Stein Ove. "Low-power microcontroller core." Thesis, Norwegian University of Science and Technology, Department of Electronics and Telecommunications, 2009. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-9048.

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<p>Energy efficiency in embedded processors is of major importance in order to achieve longer operating time for battery operated devices. In this thesis the energy efficiency of a microcontroller based on the open source ZPU microprocessor is evaluated and improved. The ZPU microprocessor is a zero-operand stack machine originally designed for small size FPGA implementation, but in this thesis the core is synthesized for implementation with a 180nm technology library. Power estimation of the design is done both before and after synthesis in the design flow, and it is shown that power estimate

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17

Fogarty, Matthew Hugh. "Fate-mapping the mouse neural tube by Cre-loxP transgenesis." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1445454/.

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The mammalian central nervous system (CNS) develops from a thin layer of neuroepithelial stem cells that form the early neural tube. Initially neuroepithelial cells generate neurons, the identity of which depends on their position of origin along the embryonic axis. Glia are generated at the end of neuronogenesis and very little is known about their specification. One of the challenges of modern developmental biology is to understand the extent of the diversity of glial cell types found in the CNS and how this diversity is generated. In this thesis I describe work using Cre-loxP technology com

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18

Emambokus, Nikla R. "Applications of the Cre-LoxP technology to the study of megakaryocytes." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325900.

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19

Hartley, O. "Using the loxP/Cre site-specific recombination system in phage display technology." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603815.

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A technique for highly efficient combinatorial transformation of heavy and light chain repertoires (combinatorial infection and <I>in vivo </I>recombination) was used to create a phage antibody library of previously unattainable diversity (6.5 x 10<SUP>10</SUP>). From this library, antibody fragments of high affinity (up to 3 nM) were isolated directly, providing experimental proof of the value of large repertoires. Subsequently a peptide display library was designed by analogy, where repertoires of ten residue 'exons' were employed in place of antibody genes. Combinatorial infection and <I>in

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20

Bradley, Lucy, and MaryLou Coffman. "Rose Care in the Low Desert." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2003. http://hdl.handle.net/10150/144749.

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21

Hochgräfe, Katja. "Cre-loxP based mouse models to study prionpathogenesis in the motor nervous system." kostenfrei, 2009. http://nbn-resolving.de/urn/resolver.pl?urn=nbn:de:bvb:20-opus-45967.

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22

Zywietz, Alexandra. "Konditionale Inaktivierung des Gna13-Gens der Maus mit Hilfe des Cre/loxP-Rekombinationssystems." [S.l.] : [s.n.], 2001. http://www.diss.fu-berlin.de/2002/113/index.html.

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23

Fernández, Carballo Blanca Leticia. "Low-cost point-of-care diagnostic devices for low resource settings." Doctoral thesis, Universitat Ramon Llull, 2017. http://hdl.handle.net/10803/401780.

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Els test de tipus 'point-of-care' (POC) presenten un gran potencial per al maneig i el diagnòstic de malalties. Els dispositius POC permeten la realització de proves clíniques prop del pacient, permetent així un diagnòstic ràpid, una ràpida iniciació de tractaments, i en cas necessari, una derivació ràpida a altres centres mèdics. Aquests dispositius tenen a més el potencial de ser més econòmics, més robustos, i més fàcils d'usar que els dispositius mèdics tradicionals. Per aquests motius, els dispositius mèdics de tipus POC es consideren prometedors per als països en vies de desenvolupament,

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24

Simpalean, Adrian Raul. "Experimental Investigations of Core-Loc Armour Units." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/38745.

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In the present study, the influence of geometric scale, unit orientation (alternatively, flow direction), and the dimensionless Reynolds and Keulegan-Carpenter quantities on the hydrodynamic loading of Core-Loc armour units is explored through a series of physical modelling tests under unsteady and oscillatory flow conditions.

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25

Picou, Frédéric. "Dissection génétique de l'action de l'hormone thyroïdienne sur la différenciation oligodendrocytaire chez la souris." Lyon, Ecole normale supérieure, 2010. http://www.theses.fr/2010ENSL0615.

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L’hormone thyroïdienne (T3) joue un rôle pleiotropique au cours du développement. Un déficit congénital en T3 provoque une pathologie nommée crétinisme, notamment caractérisée par un retard mental. In vitro, la différenciation oligodendrocytaire peut être induite par ajout de T3 au milieu de culture. Toutefois, en contradiction avec ces observations, l’inactivation des récepteurs de la T3 n’a qu’un effet marginal sur la différenciation oligodendrocytaire in vivo. Différentes lignées murines exprimant un récepteur dominant négatif de l’hormone thyroïdienne TRα1 spécifiquement dans un type cellu

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26

Steinbrecher, Kris. "INACTIVATION OF THE MOUSE GUANYLIN GENE AND ITS REGULATION DURING OSMOTIC STRESS." University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin997732948.

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27

Dennison, Paul Mark John. "The adaptation of the Cre-loxP gene disruption system for use in Candida albicans." Thesis, University of Aberdeen, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.415547.

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At the start of this project, the systems available for gene disruption in <i>C. albicans</i> were laborious and inefficient. Both alleles of a target locus need to be inactivated to construct a homozygous null mutant in this diploid fungus. This often requires the recycling of transformation markers. However, in some systems it is not possible to recycle the markers used in the disruption. This limited the creation of multiple knockout strains and the deletion of gene families. This thesis describes the successful adaptation of the Cre-<i>loxP</i> recombination system as tool for the prec

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28

Niesner, Bradley (Bradley Joseph). "Using the Cre-loxP system to randomize target gene expression states and generate diverse phenotypes." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/81684.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2013.<br>Title as it appears in MIT Commencement Exercises program, June 2013: Generation of phenotypic diversity in yeast using promoter inversion through Cre-lox recombination. Page 84 blank. Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>Modifying the expression of multiple genes enables both deeper understanding of their function and the engineering of complex multigenic cellular phenotypes. However, deletion or overexpression of multiple genes is typically laborious and

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29

Logvinoff, Carine. "Manipulations de génomes herpétiques in situ par le système Cre-loxP : aspects fondamentaux et appliqués." Lyon 1, 2000. http://www.theses.fr/2000LYO10144.

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30

Cheung, Kin. "Low-back injuries in Alberta Home care nurses." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq21259.pdf.

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31

Woodhouse, Richard. "Petrophysical Evaluations from Borehole Log and Core Measurements." Thesis, University of Bristol, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520171.

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32

Langlois, Marie-Josée. "Rôles de la phosphatase PTEN dans l'épithélium intestinal." Mémoire, Université de Sherbrooke, 2008. http://savoirs.usherbrooke.ca/handle/11143/3925.

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PTEN est une protéine dotée d'une activité phosphatase qui déphosphoryle les phosphatidylinositols issus de l'activation de la phosphatidylinositol 3-kinase (PI3K). Des mutations germinales du gène PTEN ont été mises en évidence dans le syndrome de Cowden, une maladie caractérisée par le développement de polypes le long du tube digestif et associée à un risque accru de cancer. De plus, la perte d'un allèle de Pten chez la souris conduit à la formation d'hyperplasie et de dysplasie du tractus gastro-intestinal ainsi qu'à des tumeurs notamment au niveau du côlon. Ces observations suggèrent que P

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33

Gregory, Patricia. "Self-care activities of low-income women in midlife." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0013/MQ41708.pdf.

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34

Haggas, Sarah Louise. "Oceanic crust evolution : constraints from integrated core-log studies." Thesis, University of Leicester, 1999. http://hdl.handle.net/2381/30434.

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35

Kozlowski, Tomasz. "Wave-Induced Loading of Submerged Core-Loc Armour Units." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/41870.

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This study investigates the relationship between wave-induced hydrodynamics and the resulting loading on Core-Loc concrete armour units below the still water level in a breakwater structure. Physical modelling experiments were performed at the National Research Council in Ottawa in which a 3D-printed 12 cm Core-Loc armour unit was instrumented and fixed in place within a rubble mound structure. Testing featured simultaneous measurement of force on this instrumented unit, pressure head at the base of the unit, and flow velocities below the SWL. Two main scenarios were tested, the isolated uni

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36

Hart, Margaret Sue. "Stability of child care in rural low-income families." The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1154613258.

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37

Graciolli, Vinicius Medeiros. "A novel classification method applied to well log data calibrated by ontology based core descriptions." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/174993.

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Um método para a detecção automática de tipos litológicos e contato entre camadas foi desenvolvido através de uma combinação de análise estatística de um conjunto de perfis geofísicos de poços convencionais, calibrado por descrições sistemáticas de testemunhos. O objetivo deste projeto é permitir a integração de dados de rocha em modelos de reservatório. Os testemunhos são descritos com o suporte de um sistema de nomenclatura baseado em ontologias que formaliza extensamente uma grande gama de atributos de rocha. As descrições são armazenadas em um banco de dados relacional junto com dados de p

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38

Alalasundaram, Sudhersena. "Effect of child care subsidies on price and quality of care for low -income families." FIU Digital Commons, 1997. http://digitalcommons.fiu.edu/etd/1181.

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This dissertation examines the effect of regulations, resource and referral agencies, and subsidies on price and quality of care in child care centers. This research is based on a carefully developed conceptual framework that incorporates the factors affecting the demand and supply of child care. The first step in developing this framework is sketching out the structural equations. The structural equations help us understand the underlying behavior of individuals and firms making a decision. The exogenous variables are vector of attributes relating to family characteristics, child characterist

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39

Abramowitz, Peter Prep. "Forming nitrides with low-energy ions on low-K dielectrics /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.

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40

Kwabi-Addo, Bernard. "Cre-loxP mediated genoinic targeting to develop rapid and reproducible expression of recombinant proteins in mammalian cells." Thesis, Queen Mary, University of London, 1997. http://qmro.qmul.ac.uk/xmlui/handle/123456789/25543.

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Expression levels of transgenes in mammalian cells show extreme variability between individual clones isolated from a single transfection. This is due to differing number of copies integrated into the genome and also from chromosomal "position effects". Therefore extensive screening is required to isolate a suitable cell line for high level expression of a recombinant protein. In this work, the Cre-loxP site-specific recombination system was investigated to eliminate such problems by directing the rapid targeting of any input DNA to a single pre-selected site in Chinese hamster ovary (CHO) cel

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41

Leung, Ray K. M. "Investigation of T-cell lineage commitment via Cre-loxP mediated deletion of silencer in murine CD4 gene." Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393599.

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42

Kim, Jeongrae. "Simulation study of a low-low satellite-to-satellite tracking mission /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.

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43

Burk, Laci Ann. "Barriers to accessing prenatal care in low income rural women." Thesis, Montana State University, 2012. http://etd.lib.montana.edu/etd/2012/burk/BurkL0512.pdf.

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Barriers exist to accessing prenatal care for low income women throughout the United States, such as scheduling appointments, finding childcare, and paying for prenatal care. Women who reside in rural areas experience barriers to accessing prenatal care unique to their geographical locations. Barriers to accessing prenatal care have been associated with less than optimal health outcomes for both women and infants. The purpose of this study was to explore the barriers to accessing prenatal care that are specific to low income rural women. A qualitative approach was used by conducting telephone

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44

ITO, Hideo, and Gang ZENG. "Low-Cost IP Core Test Using Tri-Template-Based Codes." Institute of Electronics, Information and Communication Engineers, 2007. http://hdl.handle.net/2237/15029.

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Gonciari, Paul Theo. "Low cost test for core-based system-on-a-chip." Thesis, University of Southampton, 2003. https://eprints.soton.ac.uk/257354/.

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The availability of high level integration leads to building of millions of gates systemson- a-chip (SOC). Due to the high complexity of SOCs, testing them is becoming increasingly difficult. In addition, if the current test practises are maintained, the high cost of test will lead to a considerable production cost increase. To alleviate the test cost problem, this research investigates methods which lead to low-cost test of core-based systems-on-a-chip based on test resource partitioning and without changing the embedded cores. Analysing the factors which drive the continuous increase in test

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46

Lima, Cicero Zanetti de. "Impacts of low carbon agriculture in Brazil: a CGE application." Universidade Federal de Viçosa, 2017. http://www.locus.ufv.br/handle/123456789/19866.

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Submitted by Marco Antônio de Ramos Chagas (mchagas@ufv.br) on 2018-05-29T12:21:20Z No. of bitstreams: 1 texto completo.pdf: 3839421 bytes, checksum: ee73ea3cd4e758f2116138315029efe9 (MD5)<br>Made available in DSpace on 2018-05-29T12:21:20Z (GMT). No. of bitstreams: 1 texto completo.pdf: 3839421 bytes, checksum: ee73ea3cd4e758f2116138315029efe9 (MD5) Previous issue date: 2017-08-22<br>Fundação de Amparo à Pesquisa do Estado de Minas Gerais<br>Além da relevância econômica, o setor agrícola fez com que o Brasil assumisse um papel ativo na discussão internacional das mudanças climáticas. O

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47

Qi, Yangjie. "FPGA Based High Throughput Low Power Multi-core Neuromorphic Processor." University of Dayton / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1449526140.

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48

Alam, Mahmood. "Development of vacuum insulation panel with low cost core material." Thesis, Brunel University, 2015. http://bura.brunel.ac.uk/handle/2438/11658.

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Buildings consume around half of the UK's total energy consumption and are responsible for almost 50% of UK's total carbon dioxide (CO2) emissions. Use of high thermal resistance insulation in buildings is critical to save the substantial amounts of space heating energy lost through building fabric. Conventional building insulation materials have higher thermal conductivity values ranging from 40 mWm-1K-1 (Glass fibre) - 26 mWm-1K-1 (Polyurethane foam) and require larger thicknesses to achieve stringent building regulation requirements which may not be feasible due to techno-economic constrain

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49

Kalanga, Noel. "Optimizing Care for Low Birth Weight Infants in Rural Malawi." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:17613732.

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The aim of our study was to explore and understand the factors that affect growth of low birth weight (LBW) infants in a rural district of Neno in Malawi in order to optimize their care. We surveyed 64 households of LBW infants born between April and June 2014. We collected quantitative data on socio-demographics and food security, and performed nutrition and development assessments of the LBW infants at six months of age. We also conducted qualitative in-depth interviews with a subset of 10 mothers of the LBW infants and with 3 nurses at postnatal clinics. We then merged the quantitative and

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50

Bozeman, Laura Ann. "The fidelity of low vision simulator systems in clinical and functional settings /." Digital version accessible at:, 1998. http://wwwlib.umi.com/cr/utexas/main.

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