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Journal articles on the topic 'Cre Lox'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

Lee, Linda, and Paul D. Sadowski. "Lox and Cre sandwich." Nature Chemical Biology 1, no. 5 (2005): 246–47. http://dx.doi.org/10.1038/nchembio1005-246.

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2

Becher, B., A. Waisman, and L. F. Lu. "Cre-lox: Target Sensitivity Matters." Immunity 51, no. 4 (2019): 595. http://dx.doi.org/10.1016/j.immuni.2019.09.012.

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3

Gewin, Leslie S. "The Cre/lox system: Cre-ating unintended damage." American Journal of Physiology-Renal Physiology 316, no. 5 (2019): F873—F874. http://dx.doi.org/10.1152/ajprenal.00428.2018.

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4

Gilbertson, Larry. "Cre–lox recombination: Cre-ative tools for plant biotechnology." Trends in Biotechnology 21, no. 12 (2003): 550–55. http://dx.doi.org/10.1016/j.tibtech.2003.09.011.

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5

Testa, Giuseppe, and A. Francis Stewart. "Cre ating a trans lox ation." EMBO reports 1, no. 2 (2000): 120–21. http://dx.doi.org/10.1093/embo-reports/kvd035.

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6

Cui, Yunxia, Lu Huang, Florent Elefteriou, et al. "Essential Role of STAT3 in Body Weight and Glucose Homeostasis." Molecular and Cellular Biology 24, no. 1 (2004): 258–69. http://dx.doi.org/10.1128/mcb.24.1.258-269.2004.

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ABSTRACT STAT3 is a ubiquitous transcription factor that is indispensable during early embryogenesis. To study the functions of STAT3 postnatally, we generated conditional STAT3-deficient mice. To that end, STAT3lox/lox mice were crossed with mice expressing Cre under the control of rat insulin II gene promoter (RIP-Cre mice). Immunohistochemical and Western blot analyses showed that STAT3 is deleted from β cells in the islets of Langerhans. Genomic DNA PCR revealed that STAT3 deletion also occurred in the hypothalamus. Hypothalamic Cre expression was further confirmed by crossing RIP-Cre/STAT

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7

Parrish, Diana C., Eric N. Alston, Hermann Rohrer та ін. "Absence of gp130 in dopamine β-hydroxylase-expressing neurons leads to autonomic imbalance and increased reperfusion arrhythmias". American Journal of Physiology-Heart and Circulatory Physiology 297, № 3 (2009): H960—H967. http://dx.doi.org/10.1152/ajpheart.00409.2009.

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Inflammatory cytokines that act through glycoprotein (gp)130 are elevated in the heart after myocardial infarction and in heart failure. These cytokines are potent regulators of neurotransmitter and neuropeptide production in sympathetic neurons but are also important for the survival of cardiac myocytes after damage to the heart. To examine the effect of gp130 cytokines on cardiac nerves, we used gp130DBH-Cre/lox mice, which have a selective deletion of the gp130 cytokine receptor in neurons expressing dopamine β-hydroxylase (DBH). Basal sympathetic parameters, including norepinephrine (NE) c

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Suzuki, Nobuaki, Hiroshi Nonaka, Yota Tsuge, Masayuki Inui, and Hideaki Yukawa. "New Multiple-Deletion Method for the Corynebacterium glutamicum Genome, Using a Mutant lox Sequence." Applied and Environmental Microbiology 71, no. 12 (2005): 8472–80. http://dx.doi.org/10.1128/aem.71.12.8472-8480.2005.

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ABSTRACT Due to the difficulty of multiple deletions using the Cre/loxP system, a simple, markerless multiple-deletion method based on a Cre/mutant lox system combining a right-element (RE) mutant lox site with a left-element (LE) mutant lox site was employed for large-scale genome rearrangements in Corynebacterium glutamicum. Eight distinct genomic regions that had been identified previously by comparative analysis of C. glutamicum R and C. glutamicum 13032 genomes were targeted for deletion. By homologous recombination, LE and RE mutant lox sites were integrated at each end of a target regio

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9

Yan, Xin, Hao-Jie Yu, Qing Hong, and Shun-Peng Li. "Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis." Applied and Environmental Microbiology 74, no. 17 (2008): 5556–62. http://dx.doi.org/10.1128/aem.01156-08.

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ABSTRACT We have developed a fast and accurate method to engineer the Bacillus subtilis genome that involves fusing by PCR two flanking homology regions with an antibiotic resistance gene cassette bordered by two mutant lox sites (lox71 and lox66). The resulting PCR products were used directly to transform B. subtilis, and then transient Cre recombinase expression in the transformants was used to recombine lox71 and lox66 into a double-mutant lox72 site, thereby excising the marker gene. The mutation process could also be accomplished in 2 days by using a strain containing a cre isopropyl-β-d-

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10

Lambert, Jolanda M., Roger S. Bongers, and Michiel Kleerebezem. "Cre-lox-Based System for Multiple Gene Deletions and Selectable-Marker Removal in Lactobacillus plantarum." Applied and Environmental Microbiology 73, no. 4 (2006): 1126–35. http://dx.doi.org/10.1128/aem.01473-06.

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ABSTRACT The classic strategy to achieve gene deletion variants is based on double-crossover integration of nonreplicating vectors into the genome. In addition, recombination systems such as Cre-lox have been used extensively, mainly for eukaryotic organisms. This study presents the construction of a Cre-lox-based system for multiple gene deletions in Lactobacillus plantarum that could be adapted for use on gram-positive bacteria. First, an effective mutagenesis vector (pNZ5319) was constructed that allows direct cloning of blunt-end PCR products representing homologous recombination target re

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11

Tsai, Ching-Yi, Yan-Yuen Poon, Chang-Han Chen, and Samuel H. H. Chan. "Anomalous baroreflex functionality inherent in floxed and Cre-Lox mice: an overlooked physiological phenotype." American Journal of Physiology-Heart and Circulatory Physiology 313, no. 4 (2017): H700—H707. http://dx.doi.org/10.1152/ajpheart.00346.2017.

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The last two decades have seen the emergence of Cre-Lox recombination as one of the most powerful and versatile technologies for cell-specific genetic engineering of mammalian cells. Understandably, the primary concerns in the practice of Cre-Lox recombination are whether the predicted genome has been correctly modified and the targeted phenotypes expressed. Rarely are the physiological conditions of the animals routinely examined because the general assumption is that they are normal. Based on corroborative results from radiotelemetric recording, power spectral analysis, and magnetic resonanc

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12

Lee, Young-sam, Sung-tae Kim, Gyoung-won Kim, Minhyung Lee, and Jong-sang Park. "An engineeredloxsequence containing part of a long terminal repeat of HIV-1 permits Cre recombinase-mediated DNA excision." Biochemistry and Cell Biology 78, no. 6 (2000): 653–58. http://dx.doi.org/10.1139/o00-067.

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In our previous report, one 34-bp sequence from a long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) clone, loxLTR-1, was proposed as a target site for site-specific excision by modified Cre recombinase. To support this suggestion, an engineered lox sequence, designated loxIL1, was made. This variant lox has the corresponding sequence of loxLTR-1 at the spacer region and the last two bases of inverted repeat sequence. Through in vitro recombination assay, loxIL1 also allowed the wild-type Cre to specifically recombine the sequence. An in vitro DNA binding experiment with

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13

Sauer, B. "Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 6 (1987): 2087–96. http://dx.doi.org/10.1128/mcb.7.6.2087.

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The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate tha

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14

Sauer, B. "Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 6 (1987): 2087–96. http://dx.doi.org/10.1128/mcb.7.6.2087-2096.1987.

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The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate tha

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15

Ventura, A., A. Meissner, C. P. Dillon, et al. "Cre-lox-regulated conditional RNA interference from transgenes." Proceedings of the National Academy of Sciences 101, no. 28 (2004): 10380–85. http://dx.doi.org/10.1073/pnas.0403954101.

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16

Paillard, Florence. "Reversible Cell Immortalization with the Cre-lox System." Human Gene Therapy 10, no. 10 (1999): 1597–98. http://dx.doi.org/10.1089/10430349950017590.

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17

Wang, Yinqiu, Piyush Tripathi, Qiusha Guo, Matthew Coussens, Liang Ma, and Feng Chen. "Cre/lox recombination in the lower urinary tract." genesis 47, no. 6 (2009): 409–13. http://dx.doi.org/10.1002/dvg.20515.

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18

Hardy, S., M. Kitamura, T. Harris-Stansil, Y. Dai, and M. L. Phipps. "Construction of adenovirus vectors through Cre-lox recombination." Journal of virology 71, no. 3 (1997): 1842–49. http://dx.doi.org/10.1128/jvi.71.3.1842-1849.1997.

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19

Smith, Lee. "Good planning and serendipity: exploiting the Cre/Lox system in the testis." REPRODUCTION 141, no. 2 (2011): 151–61. http://dx.doi.org/10.1530/rep-10-0404.

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Over the past 20 years, genetic manipulation has revolutionised our understanding of male reproductive development and function. The advent of transgenic mouse lines has permitted elegant dissection of previously intractable issues. The development of the Cre/Lox system, which has permitted spatial and temporal localisation of genetic manipulation, has expanded upon this, and now makes up one of the primary approaches underpinning our increasing understanding of testis development and function. The success of conditional gene targeting is largely reliant upon the choice of Cre recombinase expr

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20

Guo, Zhong-Min, Kang Xu, Ying Yue, et al. "Temporal Control of Cre Recombinase-mediated in Vitro DNA Recombination by Tet-on Gene Expression System." Acta Biochimica et Biophysica Sinica 37, no. 2 (2005): 133–38. http://dx.doi.org/10.1093/abbs/37.2.133.

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Abstract Conditional gene expression and gene deletion are important experimental approaches for examining the functions of particular gene products in mouse models. These strategies exploiting Cre-mediated site-specific DNA recombination have been incorporated into transgenic and gene-targeting procedures to allow in vivo manipulation of DNA in embryonic stem cells (ES cells) or living animals. The Cre/lox P system has become widely used in conditional gene targeting, conditional gene repair and activation, inducible chromosome translocation, and chromosome engineering. In this project, we ha

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21

Peoples, M., M. Golding, C. Long, and M. Westhusin. "141 DEVELOPMENT OF rLENTIVIRAL VECTORS FOR MALE GERMLINE-SPECIFIC Cre RECOMBINASE EXPRESSION IN LIVESTOCK." Reproduction, Fertility and Development 24, no. 1 (2012): 183. http://dx.doi.org/10.1071/rdv24n1ab141.

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Transgenic livestock have been used as biomedical models and have the potential to increase production characteristics. Unfortunately, some of the tools used to confirm genetic modification (transgenesis) are unacceptable in terms of public image. One key component is a fluorescent marker confirming foreign gene insert. The fluorescent protein benefits the researchers producing and selecting transgenic animals but is not required for the enhancement of the animal. Removal of the fluorescent marker can be accomplished by employing the Cre-lox recombination system. By using this system one can p

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22

Xiao, Dong, Kang Xu, Ying Yue, et al. "Temporal and Tight Hepatitis C Virus Gene Activation in Cultured Human Hepatoma Cells Mediated by a Cell-Permeable Cre Recombinase." Acta Biochimica et Biophysica Sinica 36, no. 10 (2004): 687–94. http://dx.doi.org/10.1093/abbs/36.10.687.

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Abstract Conditional gene expression has greatly facilitated the examination of the functions of particular gene products. Using the Cre/lox P switching expression system, we plan to develop efficient conditional transgene activation of hepatitis C virus core protein (HCV-C) cDNA (nucleotide 342–914) in the transgenic mice to overcome “immune tolerance” formed during the embryonic period and “immune escape” against hepatitis virus antigen in our project. To use this system in vivo, the dormant transgenic construct, i.e., pApoE-SCS-EGFP-HCV-C, was generated using techniques of standard molecula

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23

Gardenier, Jason C., Gina Farias-Eisner, Daniel Cuzzone, et al. "Inducible ablation of lymphatic vessels using Cre-lox technology." Journal of the American College of Surgeons 219, no. 4 (2014): e31. http://dx.doi.org/10.1016/j.jamcollsurg.2014.07.467.

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24

Hajdukiewicz, Peter T. J., Larry Gilbertson, and Jeffrey M. Staub. "Multiple pathways for Cre/lox-mediated recombination in plastids." Plant Journal 27, no. 2 (2001): 161–70. http://dx.doi.org/10.1046/j.1365-313x.2001.01067.x.

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25

Rubera, I. "Specific Cre/Lox Recombination in the Mouse Proximal Tubule." Journal of the American Society of Nephrology 15, no. 8 (2004): 2050–56. http://dx.doi.org/10.1097/01.asn.0000133023.89251.01.

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26

Parrish, Mark, Jay Unruh, and Robb Krumlauf. "BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology." Journal of Biomedicine and Biotechnology 2011 (2011): 1–12. http://dx.doi.org/10.1155/2011/924068.

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Bacterial Artificial Chromosomes (BACs) are vital tools in mouse genomic analyses because of their ability to propagate large inserts. The size of these constructs, however, prevents the use of conventional molecular biology techniques for modification and manipulation. Techniques such as recombineering and Cre/Lox methodologies have thus become heavily relied upon for such purposes. In this work, we investigate the applicability of Lox variant sites for serial and/or simultaneous manipulations of BACs. We show that Lox spacer mutants are very specific, and inverted repeat variants reduce Lox

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27

Wang, Lin, Sonia Rodriguez-Rodriguez, Huajia Zhang, Christen Mumaw, Angelo A. Cardoso, and Nadia Carlesso. "Activation of the Canonical Notch Signaling Pathway in the Hematopoietic Microenvironment Is Required to Maintain Normal Hematopoiesis." Blood 118, no. 21 (2011): 723. http://dx.doi.org/10.1182/blood.v118.21.723.723.

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Abstract Abstract 723 Recent studies demonstrated that the causes of Myeloproliferative syndromes (MPS) is not limited to factors intrinsic to hematopoietic cells, but include also non-cell autonomous mechanisms. Here, we demonstrate that loss of CSL-dependent Notch signaling in the hematopoietic microenvironment leads to a lethal myeloproliferative disorder. Following pIpC induction, Mx1-Cre+/RBP-Jlox/lox mice showed a significantly increase in granulocyte/macrophage progenitors and granulocytes in bone marrow (BM), peripheral blood, spleen and liver and died by week 16–20. Survival at 5 mont

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28

Hans, Stefan. "Genschere trifft Rekombinase: Konditionale Geninaktivierung geht auch einfach." Lymphoide Zellen des angeborenen Immunsystems 5, no. 2 (2021): 110–13. http://dx.doi.org/10.47184/ti.2021.02.06.

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Die konditionale Geninaktivierung wird verwendet, um ein Gen kontrolliert in einem bestimmten Gewebe oder zu einem bestimmten Zeitpunkt auszuschalten. Standartmäßig wird dafür heutzutage das Cre/lox-System benutzt. Dabei ermöglicht die Rekombinase Cre die Entfernung von DNA-Abschnitten, die von zwei sogenannten lox-Sequenzen flankiert werden. Dieser Vorgang des Flankierens ist jedoch sehr zeit- und arbeitsintensiv. Er erfordert anspruchsvolle Genomveränderungen und mehrere Generationen, bis das eigentliche Experiment der konditionalen Geninaktivierung durchgeführt werden kann. Eine neue Method

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29

Bordonaro, Michael. "Modular Cre/lox System and Genetic Therapeutics for Colorectal Cancer." Journal of Biomedicine and Biotechnology 2009 (2009): 1–12. http://dx.doi.org/10.1155/2009/358230.

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The Cre/lox system is a powerful tool for targeting therapeutic effectors in a wide variety of human disorders. I review a Cre/lox Wnt-targeted system that has shown promise against Wnt-positive colorectal cancer cell lines. In addition to Wnt-specific targeting of cell death inducers, the modular nature of this gene therapy model system can be exploited by designing positive and negative feedback loops to either amplify or inhibit Wnt activity for experimental or therapeutic benefit. I discuss the structural components and performance parameters of the system, the implication of these finding

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30

Song, Allisa J., and Richard D. Palmiter. "Detecting and Avoiding Problems When Using the Cre–lox System." Trends in Genetics 34, no. 5 (2018): 333–40. http://dx.doi.org/10.1016/j.tig.2017.12.008.

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31

Patzke, Christopher, and Thomas C. Südhof. "The conditional KO approach: Cre/Lox technology in human neurons." Rare Diseases 4, no. 1 (2016): e1131884. http://dx.doi.org/10.1080/21675511.2015.1131884.

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32

Kaneko, O. F., J. Cheng, and K. Sakamoto. "THE CONDITIONAL KNOCKDOWN OF CREB USING CRE-LOX-REGULATED SHRNA." Journal of Investigative Medicine 55, no. 1 (2007): S93. http://dx.doi.org/10.1097/00042871-200701010-00110.

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33

Hubbard, E. Jane Albert. "FLP/FRT and Cre/lox recombination technology in C. elegans." Methods 68, no. 3 (2014): 417–24. http://dx.doi.org/10.1016/j.ymeth.2014.05.007.

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34

Kirchgeorg, Lucia, Anastasia Felker, Marek van Oostrom, Elena Chiavacci, and Christian Mosimann. "Cre/lox-controlled spatiotemporal perturbation of FGF signaling in zebrafish." Developmental Dynamics 247, no. 10 (2018): 1146–59. http://dx.doi.org/10.1002/dvdy.24668.

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35

Medberry, Scott L., Emily Dale, Minmin Qin, and David W. Ow. "Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination." Nucleic Acids Research 23, no. 3 (1995): 485–90. http://dx.doi.org/10.1093/nar/23.3.485.

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36

Koshinsky, Heather A., Elsa Lee, and David W. Ow. "Cre-lox site-specific recombination between Arabidopsis and tobacco chromosomes." Plant Journal 23, no. 6 (2000): 715–22. http://dx.doi.org/10.1046/j.1365-313x.2000.00839.x.

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37

Fukushige, S. "Trapping of Mammalian Promoters by Cre-lox Site-Specific Recombination." DNA Research 3, no. 2 (1996): 73–80. http://dx.doi.org/10.1093/dnares/3.2.73.

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38

Le, Yun-Zheng. "Conditional Gene Targeting: Dissecting the Cellular Mechanisms of Retinal Degenerations." Journal of Ophthalmology 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/806783.

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Retinal neuron degeneration and survival are often regulated by the same trophic factors that are required for embryonic development and are usually expressed in multiple cell-types. Therefore, the conditional gene targeting approach is necessary to investigate the cell-specific function of widely expressed and developmentally regulated genes in retinal degeneration. The discussion in this review will be focused on the use of Cre/lox-based conditional gene targeting approach in mechanistic studies for retinal degeneration. In addition to the basic experimental designs, this article addresses v

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39

Li, Yu-Qing, and C. Shun Wong. "Metabolic Regulation of Hippocampal Neuronal Development and Its Inhibition After Irradiation." Journal of Neuropathology & Experimental Neurology 80, no. 5 (2021): 467–75. http://dx.doi.org/10.1093/jnen/nlab014.

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Abstract 5′-Adenosine monophosphate-activated protein kinase (AMPK), a key regulator of cellular energy homeostasis, plays a role in cell fate determination. Whether AMPK regulates hippocampal neuronal development remains unclear. Hippocampal neurogenesis is abrogated after DNA damage. Here, we asked whether AMPK regulates adult hippocampal neurogenesis and its inhibition following irradiation. Adult Cre-lox mice deficient in AMPK in brain, and wild-type mice were used in a birth-dating study using bromodeoxyuridine to evaluate hippocampal neurogenesis. There was no evidence of AMPK or phospho

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Khattri, Abhilasha, Soumen Nandy, and Vibha Srivastava. "Heat-inducible Cre-lox system for marker excision in transgenic rice." Journal of Biosciences 36, no. 1 (2011): 37–42. http://dx.doi.org/10.1007/s12038-011-9010-8.

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Leibig, Martina, Bernhard Krismer, Martina Kolb, Alexandra Friede, Friedrich Götz, and Ralph Bertram. "Marker Removal in Staphylococci via Cre Recombinase and Different lox Sites." Applied and Environmental Microbiology 74, no. 5 (2007): 1316–23. http://dx.doi.org/10.1128/aem.02424-07.

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ABSTRACT Allelic replacement in staphylococci is frequently aided by antibiotic resistance markers that replace the gene(s) of interest. In multiply modified strains, the number of mutated genes usually correlates with the number of selection markers in the strain's chromosome. Site-specific recombination systems are capable of eliminating such markers, if they are flanked by recombinase recognition sites. In this study, a Cre-lox setting was established that allowed the efficient removal of resistance genes from the genomes of Staphylococcus carnosus and S. aureus. Two cassettes conferring re

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Miller, Ashleigh J., Sandra D. Dudley, Jen-Lan Tsao, Darryl Shibata, and R. Michael Liskay. "Tractable Cre-lox system for stochastic alteration of genes in mice." Nature Methods 5, no. 3 (2008): 227–29. http://dx.doi.org/10.1038/nmeth.1183.

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Van Nieuwerburgh, Filip, Ryan C. Thompson, Jessica Ledesma, et al. "Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination." Nucleic Acids Research 40, no. 3 (2011): e24-e24. http://dx.doi.org/10.1093/nar/gkr1000.

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44

Harrison, David G., and Tomasz J. Guzik. "Studies of the T-Cell Angiotensin Receptor Using Cre-Lox Technology." Circulation Research 110, no. 12 (2012): 1543–45. http://dx.doi.org/10.1161/circresaha.112.271411.

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45

Sauer, Brian. "Cre/lox: One More Step in the Taming of the Genome." Endocrine 19, no. 3 (2002): 221–28. http://dx.doi.org/10.1385/endo:19:3:221.

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46

Mariscal, Ana M., Luis González-González, Enrique Querol, and Jaume Piñol. "All-in-one construct for genome engineering using Cre-lox technology." DNA Research 23, no. 3 (2016): 263–70. http://dx.doi.org/10.1093/dnares/dsw015.

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47

Liu, Hualan, David S. Robinson, Zong-Yen Wu, et al. "Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems." PLOS ONE 15, no. 11 (2020): e0241867. http://dx.doi.org/10.1371/journal.pone.0241867.

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48

Li, Qing, Kevin M. Haigis, Andrew McDaniel, et al. "Hematopoiesis and leukemogenesis in mice expressing oncogenic NrasG12D from the endogenous locus." Blood 117, no. 6 (2011): 2022–32. http://dx.doi.org/10.1182/blood-2010-04-280750.

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Abstract NRAS is frequently mutated in hematologic malignancies. We generated Mx1-Cre, Lox-STOP-Lox (LSL)-NrasG12D mice to comprehensively analyze the phenotypic, cellular, and biochemical consequences of endogenous oncogenic Nras expression in hematopoietic cells. Here we show that Mx1-Cre, LSL-NrasG12D mice develop an indolent myeloproliferative disorder but ultimately die of a diverse spectrum of hematologic cancers. Expressing mutant Nras in hematopoietic tissues alters the distribution of hematopoietic stem and progenitor cell populations, and Nras mutant progenitors show distinct respons

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49

Bestor, Aaron, Philip E. Stewart, Mollie W. Jewett, Amit Sarkar, Kit Tilly, and Patricia A. Rosa. "Use of the Cre-lox Recombination System To Investigate the lp54 Gene Requirement in the Infectious Cycle of Borrelia burgdorferi." Infection and Immunity 78, no. 6 (2010): 2397–407. http://dx.doi.org/10.1128/iai.01059-09.

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ABSTRACT Borrelia burgdorferi, the causative agent of Lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. These plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown. The conserved linear plasmid lp54 encodes several proteins important for survival in the mouse-tick infectious cycle, but the majority of the proteins are of unknown function and lack homologs outside the borreliae. In this study we adapted the Cre-lox recombination syst

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50

Hans, Stefan, Dorian Freudenreich, Michaela Geffarth, Jan Kaslin, Anja Machate, and Michael Brand. "Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish." Developmental Dynamics 240, no. 1 (2010): 108–15. http://dx.doi.org/10.1002/dvdy.22497.

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