Relevant bibliographies by topics / CRISPR / Cas9 editing

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'CRISPR / Cas9 editing'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "CRISPR / Cas9 editing"

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Watters, Kyle E., Haridha Shivram, Christof Fellmann, Rachel J. Lew, Blake McMahon, and Jennifer A. Doudna. "Potent CRISPR-Cas9 inhibitors fromStaphylococcusgenomes." Proceedings of the National Academy of Sciences 117, no. 12 (March 10, 2020): 6531–39. http://dx.doi.org/10.1073/pnas.1917668117.

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Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block CRISPR-Cas-mediated genome editing in eukaryotic cells. To identify Acrs capable of inhibitingStaphylococcus aureusCas9 (SauCas9), an alternative to the most commonly used genome editing proteinStreptococcus pyogenesCas9 (SpyCas9), we used both self-targeting CRISPR screening and guilt-by-association genomic search strategies. Here we describe three potent inhibitors of SauCas9 that we name AcrIIA13, AcrIIA14, and AcrIIA15. These inhibitors share a conserved N-terminal sequence that is dispensable for DNA cleavage inhibition and have divergent C termini that are required in each case for inhibition of SauCas9-catalyzed DNA cleavage. In human cells, we observe robust inhibition of SauCas9-induced genome editing by AcrIIA13 and moderate inhibition by AcrIIA14 and AcrIIA15. We also find that the conserved N-terminal domain of AcrIIA13–AcrIIA15 binds to an inverted repeat sequence in the promoter of these Acr genes, consistent with its predicted helix-turn-helix DNA binding structure. These data demonstrate an effective strategy for Acr discovery and establish AcrIIA13–AcrIIA15 as unique bifunctional inhibitors of SauCas9.
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Gong, Chongzhi, Shengchan Huang, Rentao Song, and Weiwei Qi. "Comparative Study between the CRISPR/Cpf1 (Cas12a) and CRISPR/Cas9 Systems for Multiplex Gene Editing in Maize." Agriculture 11, no. 5 (May 10, 2021): 429. http://dx.doi.org/10.3390/agriculture11050429.

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Although the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been proved to be an efficient multiplex gene editing system in maize, it was still unclear how CRISPR/Cpf1 (Cas12a) system would perform for multiplex gene editing in maize. To this end, this study compared the CRISPR/Cpf1 system and CRISPR/Cas9 system for multiplex gene editing in maize. The bZIP transcription factor Opaque2 (O2) was used as the target gene in both systems. We found that in the T0 and T1 generations, the CRISPR/Cpf1 system showed lower editing efficiency than the CRISPR/Cas9 system. However, in the T2 generation, the CRISPR/Cpf1 system generated more types of new mutations. While the CRISPR/Cas9 system tended to edit within the on-target range, the CRISPR/Cpf1 system preferred to edit in between the targets. We also found that in the CRISPR/Cpf1 system, the editing efficiency positively correlated with the expression level of Cpf1. In conclusion, the CRISPR/Cpf1 system offers alternative choices for target-site selection for multiplex gene editing and has acceptable editing efficiency in maize and is a valuable alternative choice for gene editing in crops.
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Saranath, Dhananjaya, and Aparna Khanna. "CRISPR/Cas9 genome editing system." Biomedical Research Journal 4, no. 2 (2017): 116. http://dx.doi.org/10.4103/2349-3666.240595.

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Dowdy, Steven F. "Controlling CRISPR-Cas9 Gene Editing." New England Journal of Medicine 381, no. 3 (July 18, 2019): 289–90. http://dx.doi.org/10.1056/nejmcibr1906886.

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Watters, Kyle E., Christof Fellmann, Hua B. Bai, Shawn M. Ren, and Jennifer A. Doudna. "Systematic discovery of natural CRISPR-Cas12a inhibitors." Science 362, no. 6411 (September 6, 2018): 236–39. http://dx.doi.org/10.1126/science.aau5138.

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Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility for synthetic genome engineering, agricultural genomics, and biomedical applications. Although bacteria harboring CRISPR-Cas9 or CRISPR-Cas3 adaptive immune systems sometimes acquire mobile genetic elements encoding anti-CRISPR proteins that inhibit Cas9, Cas3, or the DNA-binding Cascade complex, no such inhibitors have been found for CRISPR-Cas12a. Here we use a comprehensive bioinformatic and experimental screening approach to identify three different inhibitors that block or diminish CRISPR-Cas12a–mediated genome editing in human cells. We also find a widespread connection between CRISPR self-targeting and inhibitor prevalence in prokaryotic genomes, suggesting a straightforward path to the discovery of many more anti-CRISPRs from the microbial world.
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Khan, Sikandar. "Recent Advancement and Innovations in CRISPR/Cas and CRISPR Related Technologies: A review." Biotechnology and Bioprocessing 2, no. 5 (June 24, 2021): 01–12. http://dx.doi.org/10.31579/2766-2314/042.

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CRISPR genome editing technologies have been improving by every passing day. The initial CRISPR/Cas9 technologies, though emerged an improved version of genome editing in competition with TALENS and ZFNs, was nevertheless not free from technical and off-target effects. Technological improvements overtime start addressing issues with original CRISPR/Cas9 technology. The major areas of improvement targeted nucleases and delivery methods. Overtime the nuclease like Cas9 had some modifications like FokI-dCas9, Truncated guide RNAs (tru-gRNAs), Paired Cas9 nickase, Cpf1, Cas6 with Csm/Csr complex and chemically treated Cas9. In terms of delivery methods the improvements came along after almost all methods including viral methods like Recombinant Adeno Associated Viruses (rAAV), Lentivirus (LV), and bacteriophages. The review summarizes various non-viral gene delivery modes including physical methods like electroporation and chemical methods like nano particles, cell-derived membrane vesicles (CMVs) with upgraded developments. The review also compares various modes of delivering CRISPR gene editing machinery.
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Aschenbrenner, Sabine, Stefan M. Kallenberger, Mareike D. Hoffmann, Adrian Huck, Roland Eils, and Dominik Niopek. "Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity." Science Advances 6, no. 6 (February 2020): eaay0187. http://dx.doi.org/10.1126/sciadv.aay0187.

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The limited target specificity of CRISPR-Cas nucleases poses a challenge with respect to their application in research and therapy. Here, we present a simple and original strategy to enhance the specificity of CRISPR-Cas9 genome editing by coupling Cas9 to artificial inhibitory domains. Applying a combination of mathematical modeling and experiments, we first determined how CRISPR-Cas9 activity profiles relate to Cas9 specificity. We then used artificially weakened anti-CRISPR (Acr) proteins either coexpressed with or directly fused to Cas9 to fine-tune its activity toward selected levels, thereby achieving an effective kinetic insulation of ON- and OFF-target editing events. We demonstrate highly specific genome editing in mammalian cells using diverse single-guide RNAs prone to potent OFF-targeting. Last, we show that our strategy is compatible with different modes of delivery, including transient transfection and adeno-associated viral vectors. Together, we provide a highly versatile approach to reduce CRISPR-Cas OFF-target effects via kinetic insulation.
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Desai, Devam, Hiral Panchal, Shivani Patel, and Ketul Nayak. "CRISPR - CAS9 GENE EDITING: A REVIEW." International Journal of Advanced Research 8, no. 10 (October 31, 2020): 1127–32. http://dx.doi.org/10.21474/ijar01/11943.

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CRISPR is an RNA guided genome editing technique of genetic engineering which works like genetic scissors. Based on simplified version of bacterial CRISPR-Cas9 antiviral defense system. It is more accurate, faster and cost efficient than other genome editing methods. There are two components in this system: First component includes a single guide RNA (sgRNA) of system which will identify target sequence in genome and Second component will include Cas9 nuclease of system which will act as a pair of scissors to spilt the double strands of DNA. CRISPR has promising therapeutic applications. This current review focuses on mechanism, therapeutic applications, delivery systems, limitations and different approaches used for gene editing using CRISPR.
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Preece, Roland, and Christos Georgiadis. "Emerging CRISPR/Cas9 applications for T-cell gene editing." Emerging Topics in Life Sciences 3, no. 3 (April 2, 2019): 261–75. http://dx.doi.org/10.1042/etls20180144.

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Abstract Gene editing tools are being rapidly developed, accelerating many areas of cell and gene therapy research. Each successive gene editing technology promises increased efficacy, improved specificity, reduced manufacturing cost and design complexity; all of which are currently epitomised by the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas9) platform. Since its conceptualisation, CRISPR-based gene editing has been applied to existing methodologies and has further allowed the exploration of novel avenues of research. Implementation of CRISPR/Cas9 has been instrumental to recent progress in the treatment of cancer, primary immunodeficiency, and infectious diseases. To this end, T-cell therapies have attempted to harness and redirect antigen recognition function, and through gene editing, broaden T-cell targeting capabilities and enhance their potency. The purpose of this review is to provide insights into emerging applications of CRISPR/Cas9 in T-cell therapies, to briefly address concerns surrounding CRISPR-mediated indel formation, and to introduce CRISPR/Cas9 base editing technologies that hold vast potential for future research and clinical translation.
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Vaishnav, Radhika A. "The emerging role of CRISPR-Cas9 in molecular oncology." International Journal of Molecular and Immuno Oncology 2, no. 2 (June 24, 2017): 45. http://dx.doi.org/10.18203/issn.2456-3994.intjmolimmunooncol20172641.

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It is not uncommon to be curious about the recent hype surrounding the new gene editing player, Cas9, which recognizes and holds into place DNA segments known as clustered regularly interspaced short palindromic repeats (CRISPR). Together, they are known as CRISPR-Cas9 or simply “CRISPR” for brevity. The binding of Cas9 causes the CRISPR sequences to become available for editing.
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Dissertations / Theses on the topic "CRISPR / Cas9 editing"

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Roidos, Paris. "Genome editing with the CRISPR Cas9 system." Thesis, KTH, Skolan för bioteknologi (BIO), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-163694.

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Cullot, Grégoire. "Génotoxicité des systèmes CRISPR-Cas9." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0344.

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La thérapie génique est une stratégie thérapeutique prometteuse pour le traitement des maladies monogéniques. Si les premières approches, dites additives, ont reposées sur l’utilisation de vecteurs viraux, une part grandissante se tourne désormais vers l’édition génique. Celle-ci est permise par la mise au point de nouvelles générations d’endonucléases, et en particulier le système CRISPR-Cas9. Moins d’une décennie après sa caractérisation, le système CRISPR-Cas9 a permis de faire passer l’édition génique à un stade clinique. Toutefois, dans le même laps de temps, plusieurs interrogations ont été soulevées vis-à-vis de la génotoxicité pouvant être induite par la Cas9. Une littérature émergente pointe le risque de génotoxicité au site ciblé. Le travail de thèse présentée ici s’inscrit dans cette thématique. La première partie de l’étude a eu pour objectif de décrire la génotoxicité induite par une unique cassure double-brin faite par la Cas9. La caractérisation des effets a été faite à la fois à l’échelle nucléotidique, par le suivi de la balance HDR / InDels, mais également à l’échelle du chromosome. Le suivi de l’intégrité chromosomique a permis de mettre en lumière un nouveau risque de génotoxicité encore non-caractérisé. Un système de détection sensible et spécifique de ce risque a été mis au point pour continuer de le caractériser. Le second objectif a été de répondre aux limites soulevées par la génotoxicité non-voulus, en mettant au point une méthode d’édition génique plus sûre et aussi efficace, via l’utilisation d’une unique cassure simple-brin par la Cas9D10A -nickase
Gene therapy is a promising therapeutic strategy for the monogenic diseases treatment. If the first approaches, called additive, have relied on the use of viral vectors, a growing share is now turning to gene editing. Less than a decade after its characterization, the CRISPR-Cas9 system has moved gene editing to a clinical stage. However, in the same period of time, several questions have been raised regarding the genotoxicity that can be induced by Cas9. An emerging literature points to the risk of genotoxicity at the targeted site. The thesis work presented here is part of this theme. The first part of the study aimed to describe the genotoxicity induced by a single double-stranded break made by Cas9. Characterization of the effects was done both at the nucleotide level, by monitoring the HDR / InDels balance, but also at the chromosome scale. The monitoring of chromosomal integrity has brought to light a new risk of genotoxicity that was not characterized. A sensitive and specific detection system for this risk has been developed to further characterize it. The second objective was to address the limitations of unwanted genotoxicity by developing a safer and more efficient gene editing method through the use of a single single-stranded breakage by Cas9D10A-nickase
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Sousa, Maria Cristina Ferreira de. "Targeted gene editing in Neospora caninum using CRISPR/Cas9." Master's thesis, Universidade de Évora, 2021. http://hdl.handle.net/10174/29205.

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Apicomplexa are amongst the most prevalent and morbidity-causing pathogen agents worldwide, representing serious challenges to animal and public health. Neospora caninum and Besnoitia besnoiti are causing agents of neosporosis and besnoitiosis. Until today, there are no effective treatment options against these parasitosis. Therefore, it is urgent to invest in the development of methods for diagnosis, prevention, control, and treatment against these protozoan pathogens. The present dissertation is divided in two parts. The first part summarizes three assays on drug development, testing the in vitro efficacy of selected endochin-like quinolones (ELQs) against B. besnoiti and N. caninum tachyzoites on a 3-day proliferation inhibition assay, long-term experiment with the duration of 20 days, and ultrastructural changes induced by ELQs were evaluated in N. caninum. The second part of the report consists of a monography reviewing the CRISPR/Cas9 gene editing technology applied to a targeted sag1 gene knock-out in N. caninum assay; Resumo: Os parasitas do filo Apicomplexa estão entre os agentes patogénicos causadores de morbilidade mais prevalentes no mundo, representando sérios desafios para a saúde pública e animal. Neospora caninum e Besnoitia besnoiti são agentes etiológicos da neosporose e besnoitiose. Até hoje, não existem opções de tratamento e prevenção disponíveis para estas parasitoses, tornando-se urgente investir no desenvolvimento de métodos para o diagnóstico, prevenção e tratamento destes protozoários. A presente dissertação está dividido em duas partes. A primeira parte relativa a três ensaios focados no desenvolvimento de medicamentos, testa a eficácia in vitro de endoquinas tipo quinolonas contra taquizoítos de B. besnoiti e N. caninum num ensaio inibiçãoproliferação de três dias, numa experiência de tratamento de longo-curso e através de microscopia de transmissão de eletrões para avaliar alterações ultraestruturais. A segunda parte consiste numa monografia sobre a tecnologia de edição genómica CRISPR/Cas9 aplicada ao knock-out do gene sag1 em N. caninum.
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Castanon, velasco Oscar. "Targeting the transposable elements of the genome to enable large-scale genome editing and bio-containment technologies." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLX006.

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Les nucléases programmables et site-spécifiques comme CRISPR-Cas9 sont des signes avant-coureurs d’une nouvelle révolution en génie génétique et portent en germe un espoir de modification radicale de la santé humaine. Le « multiplexing » ou la capacité d’introduire plusieurs modifications simultanées dans le génome sera particulièrement utile en recherche tant fondamentale qu’appliquée. Ce nouvel outil sera susceptible de sonder les fonctions physiopathologiques de circuits génétiques complexes et de développer de meilleures thérapies cellulaires ou traitements antiviraux. En repoussant les limites du génie génétique, il sera possible d’envisager la réécriture et la conception de génomes mammifères. Le développement de notre capacité à modifier profondément le génome pourrait permettre la création de cellules résistantes aux cancers, aux virus ou même au vieillissement ; le développement de cellules ou tissus transplantables compatibles entre donneurs et receveurs ; et pourrait même rendre possible la résurrection d’espèces animales éteintes. Dans ce projet de recherche doctoral, nous présentons l’état de l’art du génie génétique « multiplex », les limites actuelles et les perspectives d’améliorations. Nous tirons profit de ces connaissances ainsi que de l’abondance des éléments transposables de notre ADN afin de construire une plateforme d’optimisation et de développement de nouveaux outils de génie génétique qui autorisent l’édition génomique à grande échelle. Nous démontrons que ces technologies permettent la production de modifications à l’échelle du génome allant jusqu’à 3 ordres de grandeur supplémentaires que précédemment, ouvrant la voie au développement de la réécriture des génomes de mammifères. En outre, l’observation de la toxicité engendrée par la multitude de coupures double-brins dans le génome nous a amenés à développer un bio-interrupteur susceptible d’éviter les effets secondaires des thérapies cellulaires actuelles ou futures. Enfin, en conclusion, nous exposons les potentielles inquiétudes et menaces qu’apporte le domaine génie génétiques et apportons des pistes de réflexions pour diminuer les risques identifiés
Programmable and site-specific nucleases such as CRISPR-Cas9 have started a genome editing revolution, holding hopes to transform human health. Multiplexing or the ability to simultaneously introduce many distinct modifications in the genome will be required for basic and applied research. It will help to probe the physio-pathological functions of complex genetic circuits and to develop improved cell therapies or anti-viral treatments. By pushing the boundaries of genome engineering, we may reach a point where writing whole mammalian genomes will be possible. Such a feat may lead to the generation of virus-, cancer- or aging- free cell lines, universal donor cell therapies or may even open the way to de-extinction. In this doctoral research project, I outline the current state-of-the-art of multiplexed genome editing, the current limits and where such technologies could be headed in the future. We leveraged this knowledge as well as the abundant transposable elements present in our DNA to build an optimization pipeline and develop a new set of tools that enable large-scale genome editing. We achieved a high level of genome modifications up to three orders of magnitude greater than previously recorded, therefore paving the way to mammalian genome writing. In addition, through the observation of the cytotoxicity generated by multiple double-strand breaks within the genome, we developed a bio-safety switch that could potentially prevent the adverse effects of current and future cell therapies. Finally, I lay out the potential concerns and threats that such an advance in genome editing technology may be bringing and point out possible solutions to mitigate the risks
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Ran, Fei Ann. "CRISPR-Cas: Development and applications for mammalian genome editing." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11610.

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The ability to introduce targeted modifications into genomes and engineer model organisms holds enormous promise for biomedical and technological applications, and has driven the development of tools such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). To facilitate genome engineering in mammalian cells, we have engineered the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 programmable nuclease systems from Streptococcus pyogenes SF370 (SpCas9) and S. thermophilus LMD-9 (St1Cas9) for mouse and human cell gene editing through heterologous expression of the minimal protein and RNA components. We have demonstrated that Cas9 nucleases can be guided by several short RNAs (sgRNAs) to introduce double stranded breaks (DSB) in the mammalian genome and induce efficient, multiplexed gene modification through non-homologous end-joining-mediated indels or homology-directed repair. Furthermore, we have engineered SpCas9 into a nicking enzyme (SpCas9n) to facilitate recombination while minimizing mutagenic DNA repair processes, and show that SpCas9n can be guided by pairs of appropriately offset sgRNAs to induce DSBs with high efficiency and specificity. In collaboration with Drs. Osamu Nureki and Hiroshi Nishimasu at the University of Tokyo, we further report the crystal structure of SpCas9 in complex with the sgRNA and target DNA, and elucidate the structure-function relationship of the ribonucleoprotein complex. Finally, through a metagenomic screen of orthologs, we have identified an additional small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) that cleaves mammalian endogenous DNA with high efficiency. SaCas9 can be packaged into adeno-associated virus for effective gene modification in vivo. Together, these technologies open up exciting possibilities for applications across basic science, biotechnology, and medicine.
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Valladares, Rodrigo, and Hanna Briheim. "Metoder och tillämpningar av CRISPR-Cas9 i cancerforskning. : Samt hur CRISPR-Cas9 kan implementeras i skolundervisningen." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-166140.

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CRISPR-Cas9 är ett effektivt genredigeringsverktyg som har upptäckts på senare år. Verktyget härstammar från ett adaptivt immunförsvar hos prokaryoter. Tekniken används för att modifiera DNA hos växter, djur och människor på ett enkelt och billigt sätt. CRISPR-Cas9 har visat sig ha stor potential vid bekämpning av olika sjukdomar däribland cancer som idag är ett globalt hälsoproblem. Inom cancerforskningen ses CRISPR-Cas9 som ett lovande verktyg vid cancerterapi och läkemedelsutveckling. I denna studie sammanställer vi aktuella metoder och användningsområden med CRISPR-Cas9 inom cancerforskning. Dessutom undersöker vi hur denna form av genteknik kan lyftas upp och tillämpas i biologiundervisningen.
CRISPR-Cas9 has recently emerged as an effective genome editing tool. The tool derives from an adaptive immune system in prokaryotes. The technology is used for modification of DNA in plants, animals and humans in a simple and inexpensive way. CRISPR-Cas9 has shown great potential in fighting different diseases like cancer which today is a global health issue. It is seen as a promising tool for cancer research when it comes to cancer therapy and drug development. Here we summarize current methods and applications of CRISPR-Cas9 for cancer research. Furthermore, we explore the possibilities of introducing and applying this kind of genetic engineering in biology teaching.

Framläggning, opponering och respondering skedde skriftligt till följd av covid19.

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Lin, ChieYu. "Characterization and Optimization of the CRISPR/Cas System for Applications in Genome Engineering." Thesis, Harvard University, 2014. http://etds.lib.harvard.edu/hms/admin/view/61.

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The ability to precisely manipulate the genome in a targeted manner is fundamental to driving both basic science research and development of medical therapeutics. Until recently, this has been primarily achieved through coupling of a nuclease domain with customizable protein modules that recognize DNA in a sequence-specific manner such as zinc finger or transcription activator-like effector domains. Though these approaches have allowed unprecedented precision in manipulating the genome, in practice they have been limited by the reproducibility, predictability, and specificity of targeted cleavage, all of which are partially attributable to the nature of protein-mediated DNA sequence recognition. It has been recently shown that the microbial CRISPR-Cas system can be adapted for eukaryotic genome editing. Cas9, an RNA-guided DNA endonuclease, is directed by a 20-nt guide sequence via Watson-Crick base-pairing to its genomic target. Cas9 subsequently induces a double-stranded DNA break that results in targeted gene disruption through non-homologous end-joining repair or gene replacement via homologous recombination. Finally, the RNA guide and protein nuclease dual component system allows simultaneous delivery of multiple guide RNAs (sgRNA) to achieve multiplex genome editing with ease and efficiency. The potential effects of off-target genomic modification represent a significant caveat to genome editing approaches in both research and therapeutic applications. Prior work from our lab and others has shown that Cas9 can tolerate some degree of mismatch with the guide RNA to target DNA base pairing. To increase substrate specificity, we devised a technique that uses a Cas9 nickase mutant with appropriately paired guide RNAs to efficiently inducing double-stranded breaks via simultaneous nicks on both strands of target DNA. As single-stranded nicks are repaired with high fidelity, targeted genome modification only occurs when the two opposite-strand nicks are closely spaced. This double nickase approach allows for marked reduction of off-target genome modification while maintaining robust on-target cleavage efficiency, making a significant step towards addressing one of the primary concerns regarding the use of genome editing technologies. The ability to multiplex genome engineering by simply co-delivering multiple sgRNAs is a versatile property unique to the CRISPR-Cas system. While co-transfection of multiple guides is readily feasible in tissue culture, many in vivo and therapeutic applications would benefit from a compact, single vector system that would allow robust and reproducible multiplex editing. To achieve this, we first generated and functionally validated alternate sgRNA architectures to characterize the structure-function relationship of the Cas9 protein with the sgRNA in DNA recognition and cleavage. We then applied this knowledge towards the development and optimization of a tandem synthetic guide RNA (tsgRNA) scaffold that allows for a single promoter to drive expression of a single RNA transcript encoding two sgRNAs, which are subsequently processed into individual active sgRNAs.
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Rodríguez, José A. "Genetic editing with CRISPR/Cas9: A scientific, ethical, and pastoral approach." Thesis, Boston College, 2019. http://hdl.handle.net/2345/bc-ir:108890.

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Thesis advisor: Andrea Vicini
Thesis advisor: Colleen M. Griffith
Thesis (STL) — Boston College, 2019
Submitted to: Boston College. School of Theology and Ministry
Discipline: Sacred Theology
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Cui, Xiucheng. "Targeted Gene Editing Using CRISPR/Cas9 in a Wheat Protoplast System." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36543.

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The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become a promising tool for targeted gene editing in a variety of organisms including plants. In this system, a 20 nt sequence on a single guide RNA (sgRNA) is the only gene-specific information required to modify a target gene. Fusarium head blight (FHB) is a devastating disease in wheat caused by the fungus Fusarium graminearum. The trichothecene it produces, deoxynivalenol (DON), is a major mycotoxin contaminant causing food production loss both in quality and yield. In this project, we used the CRISPR/Cas9 system to modify three wheat genes identified in previous experiments, including an ABC transporter (TaABCC6), and the Nuclear Transcription Factor X box-binding-Like 1 (TaNFXL1), both associated with FHB susceptibility, and a non-specific Lipid Transfer Protein (nsLTP) named TansLTP9.4 which correlates with FHB resistance. Two sgRNAs were designed to target each gene and were shown in an in vitro CRISPR/Cas9 assay to guide the sequence-specific cleavage with high efficiency. Another assay for CRISPR/Cas9 was established by the optimization of a wheat protoplast isolation and transformation system. Using a construct expressing a green fluorescent protein (GFP) as a positive control, estimated transformation efficiencies of about 60% were obtained with different batches of protoplasts. High-throughput sequencing of PCR amplicons from protoplasts transformed with editing constructs clearly showed that the three genes have been successfully edited with efficiencies of up to 42.2%. In addition, we also characterized by RT-qPCR the expression pattern of 10 genes in DON-treated protoplasts; seven of the genes were induced by DON in the protoplasts, consistent with their previously identified DON induction in treated wheat heads, while three genes expressed differentially between DON-treated wheat heads and protoplasts. Preliminary bioinformatics analyses showed that these differentially expressed genes are involved in different plant defense mechanisms.
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Hirosawa, Moe. "Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch." Kyoto University, 2019. http://hdl.handle.net/2433/242421.

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Books on the topic "CRISPR / Cas9 editing"

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Bhattacharya, Anjanabha, Vilas Parkhi, and Bharat Char, eds. CRISPR/Cas Genome Editing. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-42022-2.

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Modern Prometheus: Editing the Human Genome with Crispr-Cas9. Cambridge University Press, 2018.

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Yamamoto, Takashi. Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System. Springer, 2016.

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Yamamoto, Takashi. Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System. Springer, 2015.

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Yamamoto, Takashi. Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System. Springer, 2015.

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Parens, Erik, and Josephine Johnston, eds. Human Flourishing in an Age of Gene Editing. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780190940362.001.0001.

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The potential use of CRISPR-Cas9 and other new gene editing technologies to alter the DNA of human beings raises a host of questions. Some questions are about safety: Can these technologies be deployed without posing an unreasonable risk of physical harm to current and future generations? Can all physical risks be adequately assessed and responsibly managed? Gene editing technologies also raise other, equally if not more difficult, questions that touch on deeply held, personal, cultural, and societal values: Might such technologies redefine what it means to be healthy, normal, or cherished? Might they undermine relationships between parents and children or exacerbate the gap between the haves and have-nots? The broadest form of this second kind of question about the impact of gene editing on values is the focus of this book: What might gene editing—and related technologies—mean for human flourishing? An interdisciplinary group of scholars asks age-old questions about the nature and well-being of humans in the context of revolutionary new biotechnology that has the potential to change the genetic makeup of both existing people and future generations. These authors aim to help readers engage in a conversation about the ethics of gene editing. It is through this conversation that citizens can influence laws and the distribution of funding for science and medicine; that professional leaders can shape understanding and use of gene editing and related technologies by scientists, patients, and practitioners; and that individuals can make decisions about their own lives and the lives of their families.
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Bern, Christina Gabriele. Genome Editing in Zeiten Von CRISPR/Cas: Eine Rechtliche Analyse. Lang GmbH, Internationaler Verlag der Wissenschaften, Peter, 2020.

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Bhattacharya, Anjanabha, Vilas Parkhi, and Bharat Char. CRISPR/Cas Genome Editing: Strategies And Potential For Crop Improvement. Springer, 2021.

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Bern, Christina Gabriele. Genome Editing in Zeiten Von CRISPR/Cas: Eine Rechtliche Analyse. Lang GmbH, Internationaler Verlag der Wissenschaften, Peter, 2020.

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Bern, Christina Gabriele. Genome Editing in Zeiten Von CRISPR/Cas: Eine Rechtliche Analyse. Lang GmbH, Internationaler Verlag der Wissenschaften, Peter, 2020.

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Book chapters on the topic "CRISPR / Cas9 editing"

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Hoof, Jakob B., Christina S. Nødvig, and Uffe H. Mortensen. "Genome Editing: CRISPR-Cas9." In Methods in Molecular Biology, 119–32. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7804-5_11.

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Seruggia, Davide, and Lluis Montoliu. "CRISPR/Cas9 Approaches to Investigate the Noncoding Genome." In Genome Editing, 31–43. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-34148-4_2.

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Razzaq, Ali, Ghulam Mustafa, Muhammad Amjad Ali, Muhammad Sarwar Khan, and Faiz Ahmad Joyia. "CRISPR-mediated genome editing in maize for improved abiotic stress tolerance." In Molecular breeding in wheat, maize and sorghum: strategies for improving abiotic stress tolerance and yield, 405–20. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789245431.0023.

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Abstract This chapter discusses the applications of CRISPR-mediated genome editing to improve the abiotic stress tolerance (such as drought, heat, waterlogging and cold tolerance) of maize. CRISPR/Cas9 has great potential for maize genome manipulation at desired sites. By using CRISPR/Cas9-mediated genome editing, numerous genes can be targeted to produce elite maize cultivars that minimize the challenges of abiotic stresses. In the future, more precise and accurate variants of the CRISPR/Cas9 toolbox are expected to be used for maize yield improvement.
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Velusamy, Thilaga, Anjali Gowripalan, and David C. Tscharke. "CRISPR/Cas9-Based Genome Editing of HSV." In Methods in Molecular Biology, 169–83. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9814-2_9.

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Tong, Yaojun, Helene Lunde Robertsen, Kai Blin, Tilmann Weber, and Sang Yup Lee. "CRISPR-Cas9 Toolkit for Actinomycete Genome Editing." In Methods in Molecular Biology, 163–84. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7295-1_11.

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Tripathi, Leena, Valentine Otang Ntui, and Jaindra Nath Tripathi. "CRISPR-Cas9-Based Genome Editing of Banana." In Springer Protocols Handbooks, 223–36. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0616-2_14.

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Bao, Aili, Lam-Son Phan Tran, and Dong Cao. "CRISPR/Cas9-Based Gene Editing in Soybean." In Legume Genomics, 349–64. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0235-5_19.

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Nadakuduti, Satya Swathi, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, and David S. Douches. "Genome Editing in Potato with CRISPR/Cas9." In Methods in Molecular Biology, 183–201. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_14.

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Liu, Junqi, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, and Robert M. Stupar. "Genome Editing in Soybean with CRISPR/Cas9." In Methods in Molecular Biology, 217–34. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_16.

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Vachey, Gabriel, and Nicole Déglon. "CRISPR/Cas9-Mediated Genome Editing for Huntington’s Disease." In Methods in Molecular Biology, 463–81. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7825-0_21.

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Conference papers on the topic "CRISPR / Cas9 editing"

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Rastogi, Khushboo. "Rice Biofortification through CRISPR/Cas9-Multiplex Genome Editing." In ASPB PLANT BIOLOGY 2020. USA: ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1383191.

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Bridgeland, Aya. "Geme Editing Optimization in Cowpea (Vigna unguiculata) using CRISPR/Cas9." In ASPB PLANT BIOLOGY 2020. USA: ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1007286.

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Shima, K., T. Suzuki, Y. Ma, C. Mayhew, A. Sallese, B. C. Carey, P. Arumugam, and B. C. Trapnell. "CRISPR/Cas9 Genome Editing Therapy for Hereditary Pulmonary Alveolar Proteinosis." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a4004.

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Kershanskaya, O. I., Zh Kuli, A. Maulenbay, D. Nelidova, S. N. Nelidov, and J. Stephens. "NEW CRISPR/CAS9 GENE EDITING TECHNOLOGY FOR DEVELOPMENT OF AGRICULTURAL BIOTECHNOLOGY." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1434-1437.

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Кершанская, О. И., Г. Л. Есенбаева, Д. С. Нелидова, З. Н. Садуллаева, and С. Н. Нелидов. "PERSPECTIVES OF BREEDING DEVELOPMENT IN BARLEY THROUGH CRISPR/CAS9 GENOME EDITING." In Материалы I Всероссийской научно-практической конференции с международным участием «Геномика и современные биотехнологии в размножении, селекции и сохранении растений». Crossref, 2020. http://dx.doi.org/10.47882/genbio.2020.48.47.015.

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Crawford, Jack. "Targeted editing of Tobacco with Cas-CLOVER™: the clean alternative to CRISPR/Cas9 for plant geme editing." In ASPB PLANT BIOLOGY 2020. USA: ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1053452.

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Banyuls, Lucia, Daniel Pellicer, Silvia Castillo, María Magallón, María Mercedes Navarro, Amparo Escribano, and Francisco Dasí. "In vitro genome editing using CRISPR/Cas9 to edit SERPINA1 PiZ mutation." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa5411.

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Zheng, Qi, Ling-Jie Kong, Huanyu Jin, Jinling Li, and Ruby Yanru Chen-Tsai. "Abstract 663: Factors affecting genome editing using CRISPR/Cas9 in mouse model." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-663.

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Li, Xi, Wanbing Tang, Chenjie Zhou, Yulin Yang, Zhengang Peng, Wenrong Zhou, Qunsheng Ji, and Yong Cang. "Abstract 785: Application of CRISPR/Cas9 gene editing to primary T cells." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-785.

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"CRISPR/Cas9 – mediated genome editing of bread wheat to modulate heading time." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-135.

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Reports on the topic "CRISPR / Cas9 editing"

1

Bagley, Margo. Genome Editing in Latin America: CRISPR Patent and Licensing Policy. Inter-American Development Bank, July 2021. http://dx.doi.org/10.18235/0003409.

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The power and promise of genome editing, CRISPR specifically, was first realized with the discovery of CRISPR loci in the 1980s.i Since that time, CRISPR-Cas systems have been further developed enabling genome editing in virtually all organisms across the tree of life.i In the last few years, we have seen the development of a diverse set of CRISPR-based technologies that has revolutionized genome manipulation.ii Enabling a more diverse set of actors than has been seen with other emerging technologies to redefine research and development for biotechnology products encompassing food, agriculture, and medicine.ii Currently, the CRISPR community encompasses over 40,000 authors at 20,000 institutions that have documented their research in over 20,000 published and peer-reviewed studies.iii These CRISPR-based genome editing tools have promised tremendous opportunities in agriculture for the breeding of crops and livestock across the food supply chain. Potentially addressing issues associated with a growing global population, sustainability concerns, and possibly help address the effects of climate change.i These promises however, come along-side concerns of environmental and socio-economic risks associated with CRISPR-based genome editing, and concerns that governance systems are not keeping pace with the technological development and are ill-equipped, or not well suited, to evaluate these risks. The Inter-American Development Bank (IDB) launched an initiative in 2020 to understand the complexities of these new tools, their potential impacts on the LAC region, and how IDB may best invest in its potential adoption and governance strategies. This first series of discussion documents: “Genome Editing in Latin America: Regulatory Overview,” and “CRISPR Patent and Licensing Policy” are part of this larger initiative to examine the regulatory and institutional frameworks surrounding gene editing via CRISPR-based technologies in the Latin America and Caribbean (LAC) regions. Focusing on Argentina, Bolivia, Brazil, Colombia, Honduras, Mexico, Paraguay, Peru, and Uruguay, they set the stage for a deeper analysis of the issues they present which will be studied over the course of the next year through expert solicitations in the region, the development of a series of crop-specific case studies, and a final comprehensive regional analysis of the issues discovered.
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Kuiken, Todd, and Jennifer Kuzma. Genome Editing in Latin America: Regional Regulatory Overview. Inter-American Development Bank, July 2021. http://dx.doi.org/10.18235/0003410.

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The power and promise of genome editing, CRISPR specifically, was first realized with the discovery of CRISPR loci in the 1980s.3 Since that time, CRISPR-Cas systems have been further developed enabling genome editing in virtually all organisms across the tree of life.3 In the last few years, we have seen the development of a diverse set of CRISPR-based technologies that has revolutionized genome manipulation.4 Enabling a more diverse set of actors than has been seen with other emerging technologies to redefine research and development for biotechnology products encompassing food, agriculture, and medicine.4 Currently, the CRISPR community encompasses over 40,000 authors at 20,000 institutions that have documented their research in over 20,000 published and peer-reviewed studies.5 These CRISPR-based genome editing tools have promised tremendous opportunities in agriculture for the breeding of crops and livestock across the food supply chain. Potentially addressing issues associated with a growing global population, sustainability concerns, and possibly help address the effects of climate change.4 These promises however, come along-side concerns of environmental and socio-economic risks associated with CRISPR-based genome editing, and concerns that governance systems are not keeping pace with the technological development and are ill-equipped, or not well suited, to evaluate these risks. The Inter-American Development Bank (IDB) launched an initiative in 2020 to understand the complexities of these new tools, their potential impacts on the LAC region, and how IDB may best invest in its potential adoption and governance strategies. This first series of discussion documents: “Genome Editing in Latin America: Regulatory Overview,” and “CRISPR Patent and Licensing Policy” are part of this larger initiative to examine the regulatory and institutional frameworks surrounding gene editing via CRISPR-based technologies in the Latin America and Caribbean (LAC) regions. Focusing on Argentina, Bolivia, Brazil, Colombia, Honduras, Mexico, Paraguay, Peru, and Uruguay, they set the stage for a deeper analysis of the issues they present which will be studied over the course of the next year through expert solicitations in the region, the development of a series of crop-specific case studies, and a final comprehensive regional analysis of the issues discovered.
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