Relevant bibliographies by topics / CRISPR/Cas9-tagging

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  2. Dissertations / Theses
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Academic literature on the topic 'CRISPR/Cas9-tagging'

Author: Grafiati
Published: 1 April 2023
Last updated: 31 July 2025

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Journal articles on the topic "CRISPR/Cas9-tagging"

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Modaffari, Domenico, Aimée Finlayson, Yuyang Miao, Edward W. J. Wallace, and Kenneth E. Sawin. "Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system." Wellcome Open Research 9 (October 17, 2024): 602. http://dx.doi.org/10.12688/wellcomeopenres.23086.1.

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CRISPR-Cas9 systems can be used for precise genome editing in filamentous fungi, including Aspergillus nidulans. However, current CRISPR-Cas9 systems for A. nidulans rely on relatively complex or multi-step cloning methods to build a plasmid expressing both Cas9 and an sgRNA targeting a genomic locus. In this study we improve on existing plasmid-based CRISPR-Cas9 systems for Aspergilli by creating an extremely simple-to-use CRISPR-Cas9 system for A. nidulans genome editing. In our system, a plasmid containing both Cas9 and an sgRNA is assembled in a one-step Golden Gate reaction. We demonstrat
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Thöne, Fabian M. B., Nina S. Kurrle, Harald von Melchner, and Frank Schnütgen. "CRISPR/Cas9-mediated generic protein tagging in mammalian cells." Methods 164-165 (July 2019): 59–66. http://dx.doi.org/10.1016/j.ymeth.2019.02.018.

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Wang, Qiang, and Jeffrey J. Coleman. "CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum." Fungal Genetics and Biology 126 (May 2019): 17–24. http://dx.doi.org/10.1016/j.fgb.2019.02.002.

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Lin, Da-Wei, Benjamin P. Chung, Jia-Wei Huang, Xiaorong Wang, Lan Huang, and Peter Kaiser. "Microhomology-based CRISPR tagging tools for protein tracking, purification, and depletion." Journal of Biological Chemistry 294, no. 28 (2019): 10877–85. http://dx.doi.org/10.1074/jbc.ra119.008422.

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Work in yeast models has benefitted tremendously from the insertion of epitope or fluorescence tags at the native gene locus to study protein function and behavior under physiological conditions. In contrast, work in mammalian cells largely relies on overexpression of tagged proteins because high-quality antibodies are only available for a fraction of the mammalian proteome. CRISPR/Cas9-mediated genome editing has recently emerged as a powerful genome-modifying tool that can also be exploited to insert various tags and fluorophores at gene loci to study the physiological behavior of proteins i
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Lankford, Kaylee P., and John D. Hulleman. "Protocol for HiBiT tagging endogenous proteins using CRISPR-Cas9 gene editing." STAR Protocols 5, no. 2 (2024): 103000. http://dx.doi.org/10.1016/j.xpro.2024.103000.

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Beneke, Tom, Ulrich Dobramysl, Carolina Moura Costa Catta-Preta, Jeremy Charles Mottram, Eva Gluenz, and Richard Wheeler. "Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase." Wellcome Open Research 7 (December 5, 2022): 294. http://dx.doi.org/10.12688/wellcomeopenres.18575.1.

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We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
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Beneke, Tom, Ulrich Dobramysl, Carolina Moura Costa Catta-Preta, Jeremy Charles Mottram, Eva Gluenz, and Richard J. Wheeler. "Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase." Wellcome Open Research 7 (February 23, 2023): 294. http://dx.doi.org/10.12688/wellcomeopenres.18575.2.

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We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
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Li, Weicheng, Yaoyao Zhang, Katy Moffat, Venugopal Nair, and Yongxiu Yao. "V5 and GFP Tagging of Viral Gene pp38 of Marek’s Disease Vaccine Strain CVI988 Using CRISPR/Cas9 Editing." Viruses 14, no. 2 (2022): 436. http://dx.doi.org/10.3390/v14020436.

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Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. The availability of the complete sequence of the viral genome allowed for the identification of major genes associated with pathogenicity using different techniques, such as bacterial artificial chromosome (BAC) mutagenesis and the recent powerful clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-based editing system. Thus far, most studies on MDV genome editing using the CRISPR/Cas9 system have fo
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Dort, Erika N., and Richard C. Hamelin. "Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthora species." PLOS ONE 19, no. 9 (2024): e0306158. http://dx.doi.org/10.1371/journal.pone.0306158.

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Plasmid-mediated DNA transformation is a foundational molecular technique and the basis for most CRISPR-Cas9 gene editing systems. While plasmid transformations are well established for many agricultural Phytophthora pathogens, development of this technique in forest Phytophthoras is lacking. Given our long-term research objective to develop CRISPR-Cas9 gene editing in a forest pathogenic Phytophthora species, we sought to establish the functionality of polyethylene glycol (PEG)-mediated plasmid transformation in five species: P. cactorum, P. cinnamomi, P. cryptogea, P. ramorum, and P. syringa
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Torres-Garcia, Sito, Lorenza Di Pompeo, Luke Eivers, et al. "SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast." Wellcome Open Research 5 (November 24, 2020): 274. http://dx.doi.org/10.12688/wellcomeopenres.16405.1.

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The CRISPR/Cas9 system allows scarless, marker-free genome editing. Current CRISPR/Cas9 systems for the fission yeast Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into a Cas9-expressing plasmid. In addition, Cas9 endonuclease has been reported to be toxic to fission yeast when constitutively overexpressed from the strong adh1 promoter. To overcome these problems we have developed an improved system, SpEDIT, that uses a synthesised Cas9 sequence codon-optimised for S. pombe expressed from the medium strength adh15
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Dissertations / Theses on the topic "CRISPR/Cas9-tagging"

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Lindvall, Jenny. "Green and red fluorescent protein tagging of endogenous proteins in glioblastoma using the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 system." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-314151.

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Glioblastoma multiforme is the most malignant primary brain tumor that affects adults, recognized by the World Health Organization as an aggressive grade IV astrocytoma. Patients diagnosed with this type of tumor are left with a poor prognosis even with the most advanced treatment available. The cancer is quite heterogeneous and is typically categorized into four different subtypes depending on genetic aberrations and patient characteristics. Furthermore, researchers have discovered a subpopulation of glioblastoma cells, known as cancer stem cells, which are thought to be resistant to current
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DE, FLORIAN FANIA ROSSELLA. "Identification and characterization of therapeutic molecules affecting expression levels of the tumor suppressor DAB2IP in cancer." Doctoral thesis, Università degli Studi di Trieste, 2023. https://hdl.handle.net/11368/3042421.

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In tumors, the reciprocal communication between malignant cells and non-transformed stromal cells involves a variety of signaling proteins and modulators that cooperate to control proliferation, migration and apoptosis. Among them, the tumor suppressor DAB2IP, a Ras-GAP and signaling adaptor protein, modulates signal transduction in response to several extracellular stimuli, negatively regulating multiple oncogenic pathways. Accordingly, the loss of DAB2IP in tumor cells fosters metastasis and enhances chemo- and radio-resistance. DAB2IP is rarely mutated in cancer but is frequently downregula
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Ratz, Michael [Verfasser], Stefan [Akademischer Betreuer] [Gutachter] Jakobs, and Peter [Gutachter] Rehling. "CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins / Michael Ratz ; Gutachter: Stefan Jakobs, Peter Rehling ; Betreuer: Stefan Jakobs." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://d-nb.info/1121909892/34.

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Jones, Matthew Leslie. "The subnuclear localisation of Notch responsive genes." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274909.

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Title: The subnuclear localisation of Notch responsive genes. Candidate Name: Matthew Jones Notch signalling is a highly conserved cell-cell communication pathway with critical roles in metazoan development and mutations in Notch pathway components are implicated in many types of cancer. Notch is an excellent and well-studied model of biological signalling and gene regulation, with a single intracellular messenger, one receptor and two ligands in Drosophila. However, despite the limited number of chemical players involved, a striking number of different outcomes arise. Molecular studies have s
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Reinoite, Filipa Barroqueiro. "CRISPR/Cas9-mediated tagging of stem cell specific genes to study regeneration in the flatworm Macrostomum lignano." Master's thesis, 2019. http://hdl.handle.net/10316/88127.

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Dissertação de Mestrado em Biologia Celular e Molecular apresentada à Faculdade de Ciências e Tecnologia<br>A regeneração de tecidos é um processo que está dependente da capacidade de proliferação e diferenciação de células estaminais. No entanto, os mecanismos moleculares envolvidos neste processo são ainda uma incógnita, tornando ainda mais fascinante este atributo tão raro em alguns animais. Macrostomum lignano é um platelminta marinho que apresenta um conjunto qualidades únicas que fazem com que se adeqúe perfeitamente ao estudo de regeneração e células estaminais. É de fácil manutenção, t
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Ratz, Michael. "CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins." Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-002B-7CDA-C.

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Book chapters on the topic "CRISPR/Cas9-tagging"

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Xiang, Xi, Conghui Li, Xi Chen, et al. "CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9170-9_16.

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Ghosh, Sanjay, and Ji-Long Liu. "Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7339-2_15.

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Shvets, Elena, and Carolina Mendoza-Topaz. "Tagging and Deleting of Endogenous Caveolar Components Using CRISPR/Cas9 Technology." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0732-9_14.

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Beneke, Tom, and Eva Gluenz. "LeishGEdit: A Method for Rapid Gene Knockout and Tagging Using CRISPR-Cas9." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9210-2_9.

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Geny, Sylvain, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet. "Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1406-8_8.

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Ehrke-Schulz, Eric, Maren Schiwon, Claudia Hagedorn, and Anja Ehrhardt. "Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7231-9_11.

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Hawley, Robert G., and Teresa S. Hawley. "CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting." In Flow Cytometry Protocols. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3738-8_12.

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Geny, Sylvain, Simon Pichard, Alice Brion, et al. "Tagging Proteins with Fluorescent Reporters Using the CRISPR/Cas9 System and Double-Stranded DNA Donors." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1126-5_3.

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Reports on the topic "CRISPR/Cas9-tagging"

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Morin, S., L. L. Walling, Peter W. Atkinson, J. Li, and B. E. Tabashnik. ets for CRISPR/Cas9-mediated gene drive in Bemisia tabaci. United States-Israel Binational Agricultural Research and Development Fund, 2021. http://dx.doi.org/10.32747/2021.8134170.bard.

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The goal of our BARD proposal was to build both the necessary infrastructure and knowledge for using the CRISPR/Cas9-based gene drive system to control the whitefly Bemisia tabaci. Our research focused on achieving three main goals: (1) establishing a CRISPR/Cas9 gene-editing system for producing genetically-edited B. tabaci; (2) generating and testing CRISPR/Cas9-mediated mutations targeting genes that represent two gene drive strategies: population replacement and population suppression; (3) using computer modeling to optimize strategies for applying CRISPR/Cas9 to control B. tabaci populati
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