Relevant bibliographies by topics / CRISPRko Screening

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'CRISPRko Screening'

Author: Grafiati
Published: 1 April 2023
Last updated: 31 July 2025

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Journal articles on the topic "CRISPRko Screening"

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Yang, Caiting, Yu Lei, Tinglin Ren, and Mingze Yao. "The Current Situation and Development Prospect of Whole-Genome Screening." International Journal of Molecular Sciences 25, no. 1 (2024): 658. http://dx.doi.org/10.3390/ijms25010658.

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High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas
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Liu, Weiwei, Wei Wang, Zishuai Wang, et al. "CRISPR Screen Identifies the RNA-Binding Protein Eef1a1 as a Key Regulator of Myogenesis." International Journal of Molecular Sciences 25, no. 9 (2024): 4816. http://dx.doi.org/10.3390/ijms25094816.

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Skeletal muscle myogenesis hinges on gene regulation, meticulously orchestrated by molecular mechanisms. While the roles of transcription factors and non-coding RNAs in myogenesis are widely known, the contribution of RNA-binding proteins (RBPs) has remained unclear until now. Therefore, to investigate the functions of post-transcriptional regulators in myogenesis and uncover new functional RBPs regulating myogenesis, we employed CRISPR high-throughput RBP-KO (RBP-wide knockout) library screening. Through this approach, we successfully identified Eef1a1 as a novel regulatory factor in myogenes
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Joshi, Sahil, Glenn Wozniak, John Gagnon, et al. "Abstract 7034: Pooled CRISPR screening coupled with single-cell sequencing identifies modifiers of CAR T cell state in the context of chronic antigen stimulation." Cancer Research 84, no. 6_Supplement (2024): 7034. http://dx.doi.org/10.1158/1538-7445.am2024-7034.

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Abstract T cell exhaustion resulting from chronic antigen stimulation and an immunosuppressive tumor microenvironment limits the efficacy of T cell therapies in the solid tumor setting. The onset of T cell exhaustion is associated with distinct epigenetic and transcriptional changes. We hypothesized that genetic perturbations which shift T cells away from exhaustion associated states could increase the potency of immunotherapies. To this end, we utilized pooled, in vitro CRISPR/Cas9-based screening paired with deep sequencing readouts to characterize perturbation dependent T cell states in the
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Weiss, Simone, Allegra Lord, Bernhard Schmierer, et al. "Abstract 3778: Genome-Scale CRISPRa and CRISPRi screening for lncRNA drivers of prostate cancer progression." Cancer Research 83, no. 7_Supplement (2023): 3778. http://dx.doi.org/10.1158/1538-7445.am2023-3778.

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Abstract Background: Overtreatment of indolent prostate cancer (PC) and delayed treatment of aggressive PC is common due to suboptimal risk stratification tools, thus warranting identification of novel prognostic biomarkers. Although a few long non-coding RNAs (lncRNAs) with biomarker potential in PC are known, the majority of lncRNAs remain uncharacterized. Here, we aimed to identify novel lncRNA biomarker candidates. We hypothesized that strong candidates would have a functional role in driving PC progression in addition to their expression being linked to PC prognosis, and we therefore comb
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Cai, Ruijie, Runyu Lv, Xin’e Shi, Gongshe Yang, and Jianjun Jin. "CRISPR/dCas9 Tools: Epigenetic Mechanism and Application in Gene Transcriptional Regulation." International Journal of Molecular Sciences 24, no. 19 (2023): 14865. http://dx.doi.org/10.3390/ijms241914865.

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CRISPR/Cas9-mediated cleavage of DNA, which depends on the endonuclease activity of Cas9, has been widely used for gene editing due to its excellent programmability and specificity. However, the changes to the DNA sequence that are mediated by CRISPR/Cas9 affect the structures and stability of the genome, which may affect the accuracy of results. Mutations in the RuvC and HNH regions of the Cas9 protein lead to the inactivation of Cas9 into dCas9 with no endonuclease activity. Despite the loss of endonuclease activity, dCas9 can still bind the DNA strand using guide RNA. Recently, proteins wit
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Mariam, Mariam, Khizra Ikhlaq, Zulaikha Abid, et al. "Exploring CRISPR Cloning and Beyond Through a Biochemical Lens in Genetic Biotechnology." Scholars Academic Journal of Biosciences 13, no. 07 (2025): 867–76. https://doi.org/10.36347/sajb.2025.v13i07.001.

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By offering a precise, effective, and adaptable method for genome editing, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has completely transformed genetic biotechnology. Through a biochemical lens that highlights the molecular complexities behind its methods and discoveries, this review explores the complex field of CRISPR cloning and its emerging frontiers. We examine the molecular underpinnings of target identification, protospacer adjacent motif (PAM) specificity, and Cas nuclease activation, starting with the groundbreaking discovery of CRISPR-Cas systems i
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Andreatta, Francesco, Delilah Hendriks, and Benedetta Artegiani. "Human Organoids as an Emerging Tool for Genome Screenings." Annual Review of Biomedical Engineering 27, no. 1 (2025): 157–83. https://doi.org/10.1146/annurev-bioeng-103023-122327.

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Over the last decade, a plethora of organoid models have been generated to recapitulate aspects of human development, disease, tissue homeostasis, and repair. Organoids representing multiple tissues have emerged and are typically categorized based on their origin. Tissue-derived organoids are established directly from tissue-resident stem/progenitor cells of either adult or fetal origin. Starting from pluripotent stem cells (PSCs), PSC-derived organoids instead recapitulate the developmental trajectory of a given organ. Gene editing technologies, particularly the CRISPR-Cas toolbox, have great
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Evers, Bastiaan, Katarzyna Jastrzebski, Jeroen P. M. Heijmans, Wipawadee Grernrum, Roderick L. Beijersbergen, and Rene Bernards. "CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes." Nature Biotechnology 34, no. 6 (2016): 631–33. http://dx.doi.org/10.1038/nbt.3536.

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Watters, Kyle E., Christof Fellmann, Hua B. Bai, Shawn M. Ren, and Jennifer A. Doudna. "Systematic discovery of natural CRISPR-Cas12a inhibitors." Science 362, no. 6411 (2018): 236–39. http://dx.doi.org/10.1126/science.aau5138.

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Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility for synthetic genome engineering, agricultural genomics, and biomedical applications. Although bacteria harboring CRISPR-Cas9 or CRISPR-Cas3 adaptive immune systems sometimes acquire mobile genetic elements encoding anti-CRISPR proteins that inhibit Cas9, Cas3, or the DNA-binding Cascade complex, no such inhibitors have been found for CRISPR-Cas12a. Here we use a comprehensive bioinformatic and experimental screening approach to identify three different inhibitors that block or diminish CRISPR-Cas12a–mediated genome editing in hu
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Selle, Kurt, Todd R. Klaenhammer, and Rodolphe Barrangou. "CRISPR-based screening of genomic island excision events in bacteria." Proceedings of the National Academy of Sciences 112, no. 26 (2015): 8076–81. http://dx.doi.org/10.1073/pnas.1508525112.

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Genomic analysis ofStreptococcus thermophilusrevealed that mobile genetic elements (MGEs) likely contributed to gene acquisition and loss during evolutionary adaptation to milk. Clustered regularly interspaced short palindromic repeats–CRISPR-associated genes (CRISPR-Cas), the adaptive immune system in bacteria, limits genetic diversity by targeting MGEs including bacteriophages, transposons, and plasmids. CRISPR-Cas systems are widespread in streptococci, suggesting that the interplay between CRISPR-Cas systems and MGEs is one of the driving forces governing genome homeostasis in this genus.
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Dissertations / Theses on the topic "CRISPRko Screening"

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PERSICO, ILARIA. "DEALING WITH THE MAIN CHALLENGES OF FANCONI ANEMIA MOLECULAR DIAGNOSIS AND THERAPY." Doctoral thesis, Università degli Studi di Trieste, 2023. https://hdl.handle.net/11368/3042318.

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L’anemia di Fanconi (Fanconi anemia, FA) è una sindrome genetica rara causata da un difetto nella riparazione del danno al DNA con ampia eterogeneità genetica, numerose mutazioni private ed elevato tasso di mosaicismo. Tali fattori compromettono tuttora la formulazione di una diagnosi molecolare in caso di varianti di difficile caratterizzazione o significato patogenetico incerto. La FA è caratterizzata da aplasia midollare e un’aumentata suscettibilità allo sviluppo di tumori ematologici e solidi. Dato che i trattamenti attualmente disponibili si rivolgono ai soli difetti ematopoietici, è ess
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Erard, Nicolas Pascal Jean. "Optimization of molecular tools for high-throughput genetic screening." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/271895.

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Forward genetic screening allows for the identification of any genes important for a particular biological process or phenotype. While the power of this approach is broadly agreed on, the efficacy of currently available tools limits the strength of conclusions drawn from these experiments. This thesis describes a method to optimize molecular tools for high-throughput screening, both for shRNA and sgRNA based reagents. Using large shRNA efficacy datasets, we first designed an algorithm predicting the potency of shRNAs based on sequence determinants. Combined with a novel shRNA backbone that fur
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Sheel, Ankur. "Identification of Essential Genes in Hepatocellular Carcinomas using CRISPR Screening." eScholarship@UMMS, 2019. https://escholarship.umassmed.edu/gsbs_diss/1039.

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Hepatocellular carcinoma (HCC) is an aggressive subtype of liver cancer with a poor prognosis. Currently, prognosis for HCC patients remains poor as few therapies are available. The clinical need for more effective HCC treatments remains unmet partially because HCC is genetically heterogeneous and HCC driver genes amenable to targeted therapy are largely unknown. Mutations in the TP53 gene are found in ~30% of HCC patients and confer poor prognosis to patients. Identifying genes whose depletion can inhibit HCC growth, and determining the mechanisms involved, will aid the development of targete
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Rubanova, Natalia. "MasterPATH : network analysis of functional genomics screening data." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC109/document.

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Dans ce travail nous avons élaboré une nouvelle méthode de l'analyse de réseau à définir des membres possibles des voies moléculaires qui sont important pour ce phénotype en utilisant la « hit-liste » des expériences « omics » qui travaille dans le réseau intégré (le réseau comprend des interactions protéine-protéine, de transcription, l’acide ribonucléique micro-l’acide ribonucléique messager et celles métaboliques). La méthode tire des sous-réseaux qui sont construit des voies de quatre types les plus courtes (qui ne se composent des interactions protéine-protéine, ayant au minimum une inter
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Rousset, Francois. "CRISPRi screens in bacterial genomics." Electronic Thesis or Diss., Sorbonne université, 2020. http://www.theses.fr/2020SORUS373.

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La génomique chez les bactéries a connu un véritable essor au cours de la dernière décennie grâce aux progrès des méthodes de séquençage de l'ADN. De nouvelles techniques expérimentales sont nécessaires afin de mieux comprendre la fonction des gènes. La découverte des systèmes immunitaires adaptatifs CRISPR-Cas chez les bactéries a conduit au développement de nombreuses technologies pour cibler un acide nucléique de manière séquence-spécifique. En particulier, l’enzyme dCas9 peut être guidée vers une séquence d’ADN par un court ARN nommé sgRNA afin d'inhiber l'expression d'un gène de manière s
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Li, Meng. "Genetic dissection of the exit of pluripotency in mouse embryonic stem cells by CRISPR-based screening." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277552.

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The ground state naive pluripotency is established in the epiblast of the blastocyst and can be captured by culturing mouse embryonic stem cells (mESCs) with MEK and GSK3 inhibitors (2i). The transcription network that maintains pluripotency has been extensively studied with the indispensable core factors being Oct4, Sox2 and Nanog, together with other ancillary factors reinforcing the network. However, how this network is dissolved at the onset of differentiation is still not fully understood. To identify genes required for differentiation in an unbiased fashion, I conducted a genome-wide CRI
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Kaemena, Daniel Fraser. "CRISPR/Cas9 genome-wide loss of function screening identifies novel regulators of reprogramming to pluripotency." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31184.

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In 2006, Kazutoshi Takahashi and Shinya Yamanaka demonstrated the ability of four transcription factors; Oct4, Sox2, Klf4 and c-Myc to 'reprogram' differentiated somatic cells to a pluripotent state. This technology holds huge potential in the field of regenerative medicine, but reprogramming also a model system by which to the common regulators of all forced cell identity changes, for example, transdifferentiation. Despite this, the mechanism underlying reprogramming remains poorly understood and the efficiency of induced pluripotent stem cell (iPSC) generation, inefficient. One powerful meth
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Petrucci, Teresa. "Building a platform for flexible and scalable testing of genetic editors." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1143160.

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Cell-free systems allow to perform in-vitro transcription-translation reactions without requiring living organisms, revolutionising scientific research over the last decade. This allows to easily synthesise a variety of molecular components for genetic editing applications without requiring expensive and time-consuming procedures such as cell culture, animal maintenance etc. In this work, I aimed to develop a high-throughput platform for the rapid, flexible and scalable in-vitro testing of various genetic editors, such as those part of the CRISPR/Cas repertoire. I used the commercially avail
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Sczakiel, Henrike Lisa [Verfasser]. "Identifizierung Pathogenese-relevanter Kandidatengene im Hodgkin-Lymphom durch CRISPR/Cas9-basiertes knockout-Screening / Henrike Lisa Sczakiel." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2021. http://d-nb.info/1228859523/34.

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Lam, Phuong T. "Crispr/cas9-mediated genome editing of human pluripotent stem cells to advance human retina regeneration research." Miami University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1575372014701457.

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Books on the topic "CRISPRko Screening"

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Barilan, Y. Michael, Margherita Brusa, and Aaron Ciechanover, eds. Can precision medicine be personal; Can personalized medicine be precise? Oxford University Press, 2022. http://dx.doi.org/10.1093/oso/9780198863465.001.0001.

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In the medicine of the fifty years, the prevailing paradigms were the ‘biopsychosocial model’ and ‘evidence-based medicine’. The currently emerging vision is ‘personalized/precision medicine’. The two interchangeable names—personalized medicine and precision medicine—raise fundamental questions. Is increased precision an improvement in the personal aspects of care or does it risk an erosion of privacy? Do ‘precise’ and ‘personalized’ approaches marginalize public health? What are the roles of culture and society in the process of personalization? How can personalized medicine’s focus on the di
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Book chapters on the topic "CRISPRko Screening"

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Henriksson, Johan. "CRISPR Screening in Single Cells." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9240-9_23.

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Nishiga, Masataka, Lei S. Qi, and Joseph C. Wu. "CRISPRi/a Screening with Human iPSCs." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1484-6_23.

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Port, Fillip, and Michael Boutros. "Tissue-Specific CRISPR-Cas9 Screening in Drosophila." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2541-5_7.

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AbstractOver the last century research in Drosophila has resulted in many fundamental contributions to our understanding of the biology of multicellular organisms. Many of these breakthroughs have been based on the identification of novel gene functions in large-scale genetic screens. However, conventional forward-genetic screens have been limited by the random nature of mutagenesis and difficulties in mapping causal mutations, while reverse-genetic RNAi screens suffer from incomplete knockdown of gene expression. Recently developed large-scale CRISPR-Cas9 libraries promise to address these li
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DeLuca, Sophia, and Nenad Bursac. "CRISPR Library Screening in Cultured Cardiomyocytes." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2261-2_1.

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Vereecke, Elke, Katrijn Van Laere, and Tom Ruttink. "CRISPR/Cas Mutation Screening: From Mutant Allele Detection to Prediction of Protein Coding Potential." In A Roadmap for Plant Genome Editing. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-46150-7_5.

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AbstractCRISPR/Cas is a gene-editing technique that allows for the precise and specific introduction of a mutation into a DNA sequence. The outcome of a mutation on encoded protein depends on the type of mutation (deletion, insertion and/or substitution) and the position of the mutation in the DNA sequence. It can be predicted by using screening methods that are able to identify a mutation at nucleotide level. Here, several screening methods are discussed with a difference in complexity, resolution and scalability and the results are interpretated by taken into account the central dogma of the
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Carstens, Carsten P., Katherine A. Felts, and Sarah E. Johns. "Construction of CRISPR Libraries for Functional Screening." In Synthetic Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7795-6_7.

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Kang, Minjeong, Kangsan Kim, and Byung-Kwan Cho. "CRISPRi-Driven Genetic Screening for Designing Novel Microbial Phenotypes." In Synthetic Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3658-9_7.

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Webster, Daniel E., Sandrine Roulland, and James D. Phelan. "Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9151-8_16.

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Haney, Steven A. "High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7357-6_8.

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Klann, Tyler S., Gregory E. Crawford, Timothy E. Reddy, and Charles A. Gersbach. "Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7774-1_25.

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Conference papers on the topic "CRISPRko Screening"

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le Sage, Carlos, Prince Panckier, and Bendict CS Cross. "Abstract A186: Dual CRISPRi and CRISPRa screening reveals phenotypic switches in response to BRAF inhibition." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; October 26-30, 2017; Philadelphia, PA. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1535-7163.targ-17-a186.

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Cross, Benedict CS, Steffen Lawo, Tim ME Scales, et al. "Abstract B163: Genetic screening with CRISPR-Cas9: Proof and principles." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; November 5-9, 2015; Boston, MA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1535-7163.targ-15-b163.

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Tedesco, Donato, Paul Diehl, Mikhail Makhanov, Sylvain Baron, Dmitry Suchkov, and Alex Chenchik. "Abstract C161: CRISPR/Cas9 genome-wide gRNA library screening platform." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; November 5-9, 2015; Boston, MA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1535-7163.targ-15-c161.

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Tan, Jenille M., and Scott E. Martin. "Abstract 73: Exploring arrayed synthetic CRISPR for functional genomic screening." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-73.

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Li, Wanji. "Development and Application of CRISPR-Mediated Genetic Screening in Oncology." In ICBBE '20: 2020 7th International Conference on Biomedical and Bioinformatics Engineering. ACM, 2020. http://dx.doi.org/10.1145/3444884.3444909.

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Wei, Yiliang, and Christopher Vakoc. "Abstract 5161: Probing leukemia vulnerabilitiesin vivousing domain-focused CRISPR screening." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-5161.

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Wei, Yiliang, and Christopher Vakoc. "Abstract 5161: Probing leukemia vulnerabilitiesin vivousing domain-focused CRISPR screening." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-5161.

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Kim, Mi-Kyung, Yun Hwan Kim, and Sung-Yup Cho. "CRISPR/Cas9 screening for cisplatin resistance in endometrial cancer cells." In The 39th Annual Meeting of the Korean Society of Gynecologic Oncology. Korean Society of Gynecologic Oncology, 2024. http://dx.doi.org/10.3802/jgo.2024.35.s2.p6.

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Grassian, Alexandra R., Julian Fowler, Igor Feldman, et al. "Abstract B78: CRISPR pooled screening identifies differential dependencies on epigenetic pathways." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; November 5-9, 2015; Boston, MA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1535-7163.targ-15-b78.

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Vakoc, Christopher. "Abstract IA07: Cancer drug target identification using domain-focused CRISPR screening." In Abstracts: Advances in Sarcomas: From Basic Science to Clinical Translation; May 16-19, 2017; Philadelphia, PA. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1557-3265.sarcomas17-ia07.

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Reports on the topic "CRISPRko Screening"

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Negrete, Oscar, and Robert Damoiseaux. Arrayed Guide RNA Libraries for CRISPR-based Gene Knockout Screening. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1528976.

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Childress, Amy, Oscar Negrete, and Robert Damoiseaux. Development of Arrayed CRISPR-based Libraries for Functional Genomics Screening. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1528975.

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Burns, Malcom, and Gavin Nixon. Literature review on analytical methods for the detection of precision bred products. Food Standards Agency, 2023. http://dx.doi.org/10.46756/sci.fsa.ney927.

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The Genetic Technology (Precision Breeding) Act (England) aims to develop a science-based process for the regulation and authorisation of precision bred organisms (PBOs). PBOs are created by genetic technologies but exhibit changes which could have occurred through traditional processes. This current review, commissioned by the Food Standards Agency (FSA), aims to clarify existing terminologies, explore viable methods for the detection, identification, and quantification of products of precision breeding techniques, address and identify potential solutions to the analytical challenges presente
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