Log in

Relevant bibliographies by topics / CRISPRko Screening / Journal articles

To see the other types of publications on this topic, follow the link: CRISPRko Screening.

Journal articles on the topic 'CRISPRko Screening'

Author: Grafiati

Published: 1 April 2023

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'CRISPRko Screening.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Yang, Caiting, Yu Lei, Tinglin Ren, and Mingze Yao. "The Current Situation and Development Prospect of Whole-Genome Screening." International Journal of Molecular Sciences 25, no. 1 (2024): 658. http://dx.doi.org/10.3390/ijms25010658.

Full text

Abstract:

High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas

APA, Harvard, Vancouver, ISO, and other styles

2

Liu, Weiwei, Wei Wang, Zishuai Wang, et al. "CRISPR Screen Identifies the RNA-Binding Protein Eef1a1 as a Key Regulator of Myogenesis." International Journal of Molecular Sciences 25, no. 9 (2024): 4816. http://dx.doi.org/10.3390/ijms25094816.

Full text

Abstract:

Skeletal muscle myogenesis hinges on gene regulation, meticulously orchestrated by molecular mechanisms. While the roles of transcription factors and non-coding RNAs in myogenesis are widely known, the contribution of RNA-binding proteins (RBPs) has remained unclear until now. Therefore, to investigate the functions of post-transcriptional regulators in myogenesis and uncover new functional RBPs regulating myogenesis, we employed CRISPR high-throughput RBP-KO (RBP-wide knockout) library screening. Through this approach, we successfully identified Eef1a1 as a novel regulatory factor in myogenes

APA, Harvard, Vancouver, ISO, and other styles

3

Joshi, Sahil, Glenn Wozniak, John Gagnon, et al. "Abstract 7034: Pooled CRISPR screening coupled with single-cell sequencing identifies modifiers of CAR T cell state in the context of chronic antigen stimulation." Cancer Research 84, no. 6_Supplement (2024): 7034. http://dx.doi.org/10.1158/1538-7445.am2024-7034.

Full text

Abstract:

Abstract T cell exhaustion resulting from chronic antigen stimulation and an immunosuppressive tumor microenvironment limits the efficacy of T cell therapies in the solid tumor setting. The onset of T cell exhaustion is associated with distinct epigenetic and transcriptional changes. We hypothesized that genetic perturbations which shift T cells away from exhaustion associated states could increase the potency of immunotherapies. To this end, we utilized pooled, in vitro CRISPR/Cas9-based screening paired with deep sequencing readouts to characterize perturbation dependent T cell states in the

APA, Harvard, Vancouver, ISO, and other styles

4

Weiss, Simone, Allegra Lord, Bernhard Schmierer, et al. "Abstract 3778: Genome-Scale CRISPRa and CRISPRi screening for lncRNA drivers of prostate cancer progression." Cancer Research 83, no. 7_Supplement (2023): 3778. http://dx.doi.org/10.1158/1538-7445.am2023-3778.

Full text

Abstract:

Abstract Background: Overtreatment of indolent prostate cancer (PC) and delayed treatment of aggressive PC is common due to suboptimal risk stratification tools, thus warranting identification of novel prognostic biomarkers. Although a few long non-coding RNAs (lncRNAs) with biomarker potential in PC are known, the majority of lncRNAs remain uncharacterized. Here, we aimed to identify novel lncRNA biomarker candidates. We hypothesized that strong candidates would have a functional role in driving PC progression in addition to their expression being linked to PC prognosis, and we therefore comb

APA, Harvard, Vancouver, ISO, and other styles

5

Cai, Ruijie, Runyu Lv, Xin’e Shi, Gongshe Yang, and Jianjun Jin. "CRISPR/dCas9 Tools: Epigenetic Mechanism and Application in Gene Transcriptional Regulation." International Journal of Molecular Sciences 24, no. 19 (2023): 14865. http://dx.doi.org/10.3390/ijms241914865.

Full text

Abstract:

CRISPR/Cas9-mediated cleavage of DNA, which depends on the endonuclease activity of Cas9, has been widely used for gene editing due to its excellent programmability and specificity. However, the changes to the DNA sequence that are mediated by CRISPR/Cas9 affect the structures and stability of the genome, which may affect the accuracy of results. Mutations in the RuvC and HNH regions of the Cas9 protein lead to the inactivation of Cas9 into dCas9 with no endonuclease activity. Despite the loss of endonuclease activity, dCas9 can still bind the DNA strand using guide RNA. Recently, proteins wit

APA, Harvard, Vancouver, ISO, and other styles

6

Mariam, Mariam, Khizra Ikhlaq, Zulaikha Abid, et al. "Exploring CRISPR Cloning and Beyond Through a Biochemical Lens in Genetic Biotechnology." Scholars Academic Journal of Biosciences 13, no. 07 (2025): 867–76. https://doi.org/10.36347/sajb.2025.v13i07.001.

Full text

Abstract:

By offering a precise, effective, and adaptable method for genome editing, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has completely transformed genetic biotechnology. Through a biochemical lens that highlights the molecular complexities behind its methods and discoveries, this review explores the complex field of CRISPR cloning and its emerging frontiers. We examine the molecular underpinnings of target identification, protospacer adjacent motif (PAM) specificity, and Cas nuclease activation, starting with the groundbreaking discovery of CRISPR-Cas systems i

APA, Harvard, Vancouver, ISO, and other styles

7

Andreatta, Francesco, Delilah Hendriks, and Benedetta Artegiani. "Human Organoids as an Emerging Tool for Genome Screenings." Annual Review of Biomedical Engineering 27, no. 1 (2025): 157–83. https://doi.org/10.1146/annurev-bioeng-103023-122327.

Full text

Abstract:

Over the last decade, a plethora of organoid models have been generated to recapitulate aspects of human development, disease, tissue homeostasis, and repair. Organoids representing multiple tissues have emerged and are typically categorized based on their origin. Tissue-derived organoids are established directly from tissue-resident stem/progenitor cells of either adult or fetal origin. Starting from pluripotent stem cells (PSCs), PSC-derived organoids instead recapitulate the developmental trajectory of a given organ. Gene editing technologies, particularly the CRISPR-Cas toolbox, have great

APA, Harvard, Vancouver, ISO, and other styles

8

Evers, Bastiaan, Katarzyna Jastrzebski, Jeroen P. M. Heijmans, Wipawadee Grernrum, Roderick L. Beijersbergen, and Rene Bernards. "CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes." Nature Biotechnology 34, no. 6 (2016): 631–33. http://dx.doi.org/10.1038/nbt.3536.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Watters, Kyle E., Christof Fellmann, Hua B. Bai, Shawn M. Ren, and Jennifer A. Doudna. "Systematic discovery of natural CRISPR-Cas12a inhibitors." Science 362, no. 6411 (2018): 236–39. http://dx.doi.org/10.1126/science.aau5138.

Full text

Abstract:

Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility for synthetic genome engineering, agricultural genomics, and biomedical applications. Although bacteria harboring CRISPR-Cas9 or CRISPR-Cas3 adaptive immune systems sometimes acquire mobile genetic elements encoding anti-CRISPR proteins that inhibit Cas9, Cas3, or the DNA-binding Cascade complex, no such inhibitors have been found for CRISPR-Cas12a. Here we use a comprehensive bioinformatic and experimental screening approach to identify three different inhibitors that block or diminish CRISPR-Cas12a–mediated genome editing in hu

APA, Harvard, Vancouver, ISO, and other styles

10

Selle, Kurt, Todd R. Klaenhammer, and Rodolphe Barrangou. "CRISPR-based screening of genomic island excision events in bacteria." Proceedings of the National Academy of Sciences 112, no. 26 (2015): 8076–81. http://dx.doi.org/10.1073/pnas.1508525112.

Full text

Abstract:

Genomic analysis ofStreptococcus thermophilusrevealed that mobile genetic elements (MGEs) likely contributed to gene acquisition and loss during evolutionary adaptation to milk. Clustered regularly interspaced short palindromic repeats–CRISPR-associated genes (CRISPR-Cas), the adaptive immune system in bacteria, limits genetic diversity by targeting MGEs including bacteriophages, transposons, and plasmids. CRISPR-Cas systems are widespread in streptococci, suggesting that the interplay between CRISPR-Cas systems and MGEs is one of the driving forces governing genome homeostasis in this genus.

APA, Harvard, Vancouver, ISO, and other styles

11

Thege, Fredrik Ivar, Sonja M. Woermann, and Anirban Maitra. "Abstract 3: In vivo CRISPR activation screening identifies tissue-specific oncogene selection." Cancer Research 83, no. 7_Supplement (2023): 3. http://dx.doi.org/10.1158/1538-7445.am2023-3.

Full text

Abstract:

Abstract Background: While the genetic landscapes of pancreatic ductal adenocarcinoma (PDAC) and non-small cell lung cancer (NSCLC) are relatively well described, the contribution of individual genes and transcriptomic programs to progression and metastasis remain incompletely understood. To quantify the tissue-specific selective advantage of transcriptomic drivers of tumor progression and metastasis, we have developed a platform for in vivo CRISPR activation (CRISPRa) oncogene screening. Material and Methods: Our platform consists of an autochthonous CRISPRa-competent mouse tumor model (PPKS;

APA, Harvard, Vancouver, ISO, and other styles

12

Kampmann, Martin, Max A. Horlbeck, Yuwen Chen, et al. "Next-generation libraries for robust RNA interference-based genome-wide screens." Proceedings of the National Academy of Sciences 112, no. 26 (2015): E3384—E3391. http://dx.doi.org/10.1073/pnas.1508821112.

Full text

Abstract:

Genetic screening based on loss-of-function phenotypes is a powerful discovery tool in biology. Although the recent development of clustered regularly interspaced short palindromic repeats (CRISPR)-based screening approaches in mammalian cell culture has enormous potential, RNA interference (RNAi)-based screening remains the method of choice in several biological contexts. We previously demonstrated that ultracomplex pooled short-hairpin RNA (shRNA) libraries can largely overcome the problem of RNAi off-target effects in genome-wide screens. Here, we systematically optimize several aspects of

APA, Harvard, Vancouver, ISO, and other styles

13

Liang, Yingjuan, Xiaoxia Yao, Jingxin Han, et al. "Establishment of a CRISPR-Based Lentiviral Activation Library for Transcription Factor Screening in Porcine Cells." Animals 15, no. 1 (2024): 19. https://doi.org/10.3390/ani15010019.

Full text

Abstract:

Transcription factors play important roles in the growth and development of various tissues in pigs, such as muscle, fat, and bone. A transcription-factor-scale activation library based on the clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated endonuclease Cas9 (Cas9) system could facilitate the discovery and functional characterization of the transcription genes involved in a specific gene network. Here, we have designed and constructed a CRISPR activation (CRISPRa) sgRNA library, containing 5056 sgRNAs targeting the promoter region of 1264 transcription fac

APA, Harvard, Vancouver, ISO, and other styles

14

Tang, Lei. "Adaptable CRISPR screening." Nature Methods 21, no. 12 (2024): 2226. https://doi.org/10.1038/s41592-024-02544-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Thege, Fredrik I., Sonja M. Woermann, and Anirban Maitra. "Abstract C057: Delineating tissue-specific oncogene selection with autochthonous CRISPR activation screening." Cancer Research 84, no. 2_Supplement (2024): C057. http://dx.doi.org/10.1158/1538-7445.panca2023-c057.

Full text

Abstract:

Abstract Background: While the genetic landscapes of pancreatic ductal adenocarcinoma (PDAC) and non-small cell lung cancer (NSCLC) are well described, the contribution of individual genes and transcriptomic programs to tumor initiation, progression and metastasis remain incompletely understood. To quantify the oncogenic potential of individual genes and identify transcriptomic programs that drive progression and metastasis, we have developed a platform for in vivo CRISPR activation (CRISPRa) screening that allows us to delineate tissue-specific oncogene selection. Material and Methods: Our pl

APA, Harvard, Vancouver, ISO, and other styles

16

Salgado, Alexandra Mariel Vázquez, Shirui He, and Kirk J. Wangensteen. "Abstract 250: Identifying drivers of liver cancer using CRISPR activation screening in vivo." Cancer Research 83, no. 7_Supplement (2023): 250. http://dx.doi.org/10.1158/1538-7445.am2023-250.

Full text

Abstract:

Abstract Hepatocellular Carcinoma (HCC) is the most prevalent type of liver cancer and the third leading cause of cancer deaths worldwide. Drugs approved for first- and second-line therapy extend survival by only several months. Hence, there is still a pressing need for new and effective treatments. Sequencing technologies applied to human HCC tissues have identified hundreds of genes that undergo amplification and that may be correlated with mortality. Nevertheless, correlation does not equate to functionality. We aim to annotate gene function by employing an innovative approach with Clustere

APA, Harvard, Vancouver, ISO, and other styles

17

Kitada, Sayaka, Yoko Nishinaka-Arai, Momoko Nishikori, Akira Niwa, and Megumu K. Saito. "Screening for Intracellular Phosphorylation Cascades That Positively and Negatively Regulate the Self-Renewing Proliferation of Immature HPCs." Blood 142, Supplement 1 (2023): 4055. http://dx.doi.org/10.1182/blood-2023-183086.

Full text

Abstract:

For the purpose of studying hematological diseases involving complex lineages of cells, hematopoietic differentiation using patient-derived iPS cells has already proved to be powerful in the analysis of various diseases. However, in terms of sourcing for the expansion of its use to larger scale studies, the time-consuming and inefficient differentiation process still presents a significant obstacle. In this context, the development of a method that allows the proliferation of progenitor cells (HPCs) which maintains their ability to differentiate into multiple lineages is an important issue. In

APA, Harvard, Vancouver, ISO, and other styles

18

Göttl, Vanessa L., Ina Schmitt, Kristina Braun, Petra Peters-Wendisch, Volker F. Wendisch, and Nadja A. Henke. "CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production by Corynebacterium glutamicum." Microorganisms 9, no. 4 (2021): 670. http://dx.doi.org/10.3390/microorganisms9040670.

Full text

Abstract:

Corynebacterium glutamicum is a prominent production host for various value-added compounds in white biotechnology. Gene repression by dCas9/clustered regularly interspaced short palindromic repeats (CRISPR) interference (CRISPRi) allows for the identification of target genes for metabolic engineering. In this study, a CRISPRi-based library for the repression of 74 genes of C. glutamicum was constructed. The chosen genes included genes encoding enzymes of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle, regulatory genes, as well as genes of the methylerythritol phos

APA, Harvard, Vancouver, ISO, and other styles

19

Lanning, Bryan R., and Christopher R. Vakoc. "Single-minded CRISPR screening." Nature Biotechnology 35, no. 4 (2017): 339–40. http://dx.doi.org/10.1038/nbt.3849.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

GÜLER KARA, Hale, Buket KOSOVA, Eda DOĞAN, Vildan BOZOK ÇETİNTAŞ, and Şerif ŞENTÜRK. "CRISPR-Cas Functional Genetic Screening: Traditional Review." Turkiye Klinikleri Journal of Medical Sciences 42, no. 4 (2022): 311–22. http://dx.doi.org/10.5336/medsci.2022-88507.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Haswell, Jeffrey R., Kaia Mattioli, Chiara Gerhardinger, et al. "Genome-wide CRISPR interference screen identifies long non-coding RNA loci required for differentiation and pluripotency." PLOS ONE 16, no. 11 (2021): e0252848. http://dx.doi.org/10.1371/journal.pone.0252848.

Full text

Abstract:

Although many long non-coding RNAs (lncRNAs) exhibit lineage-specific expression, the vast majority remain functionally uncharacterized in the context of development. Here, we report the first described human embryonic stem cell (hESC) lines to repress (CRISPRi) or activate (CRISPRa) transcription during differentiation into all three germ layers, facilitating the modulation of lncRNA expression during early development. We performed an unbiased, genome-wide CRISPRi screen targeting thousands of lncRNA loci expressed during endoderm differentiation. While dozens of lncRNA loci were required fo

APA, Harvard, Vancouver, ISO, and other styles

22

Ancos-Pintado, Raquel, Irene Bragado-García, María Luz Morales, et al. "High-Throughput CRISPR Screening in Hematological Neoplasms." Cancers 14, no. 15 (2022): 3612. http://dx.doi.org/10.3390/cancers14153612.

Full text

Abstract:

CRISPR is becoming an indispensable tool in biological research, revolutionizing diverse fields of medical research and biotechnology. In the last few years, several CRISPR-based genome-targeting tools have been translated for the study of hematological neoplasms. However, there is a lack of reviews focused on the wide uses of this technology in hematology. Therefore, in this review, we summarize the main CRISPR-based approaches of high throughput screenings applied to this field. Here we explain several libraries and algorithms for analysis of CRISPR screens used in hematology, accompanied by

APA, Harvard, Vancouver, ISO, and other styles

23

Serebrenik, Yevgeniy V., and Ophir Shalem. "CRISPR mutagenesis screening of mice." Nature Cell Biology 20, no. 11 (2018): 1235–37. http://dx.doi.org/10.1038/s41556-018-0224-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Lau, Esther. "CRISPR screening from both ways." Nature Reviews Genetics 15, no. 12 (2014): 778–79. http://dx.doi.org/10.1038/nrg3850.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Eisenstein, Michael. "CRISPR Screening Explores New Dimensions." Genetic Engineering & Biotechnology News 40, no. 7 (2020): 26–28. http://dx.doi.org/10.1089/gen.40.07.07.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Zlotorynski, Eytan. "CRISPR–Cas screening for enhancers." Nature Reviews Molecular Cell Biology 17, no. 3 (2016): 135. http://dx.doi.org/10.1038/nrm.2016.22.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Camsund, Daniel, Michael J. Lawson, Jimmy Larsson, et al. "Time-resolved imaging-based CRISPRi screening." Nature Methods 17, no. 1 (2019): 86–92. http://dx.doi.org/10.1038/s41592-019-0629-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Wang, Chenlu, Yu Liu, Jingfei Xiong, et al. "Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction." PLOS Pathogens 20, no. 7 (2024): e1012344. http://dx.doi.org/10.1371/journal.ppat.1012344.

Full text

Abstract:

AAV-mediated gene therapy typically requires a high dose of viral transduction, risking acute immune responses and patient safety, part of which is due to limited understanding of the host-viral interactions, especially post-transduction viral genome processing. Here, through a genome-wide CRISPR screen, we identified SMCHD1 (Structural Maintenance of Chromosomes Hinge Domain 1), an epigenetic modifier, as a critical broad-spectrum restricting host factor for post-entry AAV transgene expression. SMCHD1 knock-down by RNAi and CRISPRi or knock-out by CRISPR all resulted in significantly enhanced

APA, Harvard, Vancouver, ISO, and other styles

29

Watters, Kyle E., Haridha Shivram, Christof Fellmann, Rachel J. Lew, Blake McMahon, and Jennifer A. Doudna. "Potent CRISPR-Cas9 inhibitors fromStaphylococcusgenomes." Proceedings of the National Academy of Sciences 117, no. 12 (2020): 6531–39. http://dx.doi.org/10.1073/pnas.1917668117.

Full text

Abstract:

Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block CRISPR-Cas-mediated genome editing in eukaryotic cells. To identify Acrs capable of inhibitingStaphylococcus aureusCas9 (SauCas9), an alternative to the most commonly used genome editing proteinStreptococcus pyogenesCas9 (SpyCas9), we used both self-targeting CRISPR screening and guilt-by-association genomic search strategies. Here we describe thr

APA, Harvard, Vancouver, ISO, and other styles

30

Chafe, Shawn, Kui Zhai, Nikoo Aghaei, et al. "BSBM-09 IN VIVO CRISPR ACTIVATION SCREEN IDENTIFIES ß-SITE AMYLOID PRECURSOR PROTEIN CLEAVING ENZYME 1 (BACE1) AS A DRIVER OF NON-SMALL CELL LUNG CANCER BRAIN METASTASIS." Neuro-Oncology Advances 5, Supplement_3 (2023): iii2. http://dx.doi.org/10.1093/noajnl/vdad070.005.

Full text

Abstract:

Abstract Brain metastasis occurs in up to 40% of patients with non-small cell lung cancer (NSCLC). Considerable genomic heterogeneity exists between the primary lung tumour and respective brain metastasis; however, the identity of the genes capable of driving brain metastasis is incompletely understood. Here, we carried out an in vivo genome wide CRISPR activation (CRISPRa) screen to identify molecular drivers of brain metastasis from a NSCLC patient-derived xenograft model. We identified activation of the Alzheimer’s disease associated ß-site amyloid precursor protein cleaving enzyme 1 (BACE1

APA, Harvard, Vancouver, ISO, and other styles

31

le Sage, Carlos, Steffen Lawo, and Benedict C. S. Cross. "CRISPR: A Screener’s Guide." SLAS DISCOVERY: Advancing the Science of Drug Discovery 25, no. 3 (2019): 233–40. http://dx.doi.org/10.1177/2472555219883621.

Full text

Abstract:

The discovery of CRISPR-Cas9 systems has fueled a rapid expansion of gene editing adoption and has impacted pharmaceutical and biotechnology research substantially. Here, gene editing is used at an industrial scale to identify and validate new biological targets for precision medicines, with functional genomic screening having an increasingly important role. Functional genomic strategies provide a crucial link between observed biological phenomena and the genes that influence and drive those phenomena. Although such studies are not new, the use of CRISPR-Cas9 systems in this arena is providing

APA, Harvard, Vancouver, ISO, and other styles

32

Winter, Timothy, Timothy Myers, Leandro Colli, et al. "Abstract 6154: Targeted CRISPRi screen identifies functional variants and novel target genes at multiple renal cell carcinoma (RCC) susceptibility loci." Cancer Research 84, no. 6_Supplement (2024): 6154. http://dx.doi.org/10.1158/1538-7445.am2024-6154.

Full text

Abstract:

Abstract Genome-wide association studies (GWAS) have identified 13 renal cell carcinoma (RCC) risk regions, as well as 7 regions with nominal significance, but detailed investigation of how these regions function is required to reveal the underlying biological bases of disease susceptibility. While detailed study of four loci have implicated critical pathways in RCC, causal genes and pathways for most loci remain unidentified. To identify such causative variants and target genes, we evaluated a set of variants based on Massively Parallel Reporter Assays (MPRA), eQTL analysis, capture Hi-C, and

APA, Harvard, Vancouver, ISO, and other styles

33

Yin, Zixi, and Lingyi Chen. "Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells." Stem Cells International 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/2601746.

Full text

Abstract:

The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. However, the diploid status of most mammalian cells restricts the application of CRISPR/Cas9 in genetic screening. Mammalian haploid embryonic stem cells (haESCs) have only one set of chromosomes per cell, avoiding the issue of heterozygous recessive mutations in diploid cells. Thus, the combination of haESCs and CRISPR/Cas9 facilitates the generation of genome-wide k

APA, Harvard, Vancouver, ISO, and other styles

34

Choi, Ahyoung, Insu Jang, Heewon Han, et al. "iCSDB: an integrated database of CRISPR screens." Nucleic Acids Research 49, no. D1 (2020): D956—D961. http://dx.doi.org/10.1093/nar/gkaa989.

Full text

Abstract:

Abstract High-throughput screening based on CRISPR-Cas9 libraries has become an attractive and powerful technique to identify target genes for functional studies. However, accessibility of public data is limited due to the lack of user-friendly utilities and up-to-date resources covering experiments from third parties. Here, we describe iCSDB, an integrated database of CRISPR screening experiments using human cell lines. We compiled two major sources of CRISPR-Cas9 screening: the DepMap portal and BioGRID ORCS. DepMap portal itself is an integrated database that includes three large-scale proj

APA, Harvard, Vancouver, ISO, and other styles

35

Степаненко, Liliya Stepanenko, Парамонов, et al. "BIoInfoRmatIonal analySIS of YersiniapseudotuberculosisIP32953 CRISPR/CaSSyStem." Бюллетень Восточно-Сибирского научного центра Сибирского отделения Российской академии медицинских наук 1, no. 5 (2016): 64–67. http://dx.doi.org/10.12737/23384.

Full text

Abstract:

The results of this study include Yersinia pseudotuberculosis CRISPR/Cas system structure analysis. CRISPR/Cas system is a specific adaptive protection against heterogeneous genetic elements. The object of research was the complete genome of Y. pseudotuberculosis IP32953 (NC_006155). CRISPR/Cas system screening was performed by program modelling methods MacSyFinder ver. 1.0.2. CRISPR loci screening and analyzing were carried out by program package: CRISPR Recognition tool (CRT), CRISPI: a CRISPR Interactive database, CRISPRFinder, and PilerCR. Spacer sequences were used in order to find protos

APA, Harvard, Vancouver, ISO, and other styles

36

Smieszek, Sandra, Fusun Doldur-Balli, Brendan Keenan, et al. "0041 Drug Screening and CRISPR/Cas9 Screening of HCN Channels." SLEEP 47, Supplement_1 (2024): A18—A19. http://dx.doi.org/10.1093/sleep/zsae067.0041.

Full text

Abstract:

Abstract Introduction A growing body of evidence suggests a role for HCN (hyperpolarization-activated cyclic nucleotide-gated) channels in regulation of sleep. We first tested effects of three HCN channel blockers (Corlanor, Zatebradine Hydrochloride and ZD7288) on sleep/wake behavior in wild type zebrafish compared to DMSO control. Then, to more definitively investigate roles of HCN channels, we knocked out genes encoding for HCN channel subunits in zebrafish by utilizing CRISPR/Cas9 technique and we assessed sleep/wake behavior in the HCN channel mutants. Methods The compounds were tested at

APA, Harvard, Vancouver, ISO, and other styles

37

Liu, S. John, Max A. Horlbeck, Seung Woo Cho, et al. "CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells." Science 355, no. 6320 (2016): eaah7111. http://dx.doi.org/10.1126/science.aah7111.

Full text

Abstract:

The human genome produces thousands of long noncoding RNAs (lncRNAs)—transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-mod

APA, Harvard, Vancouver, ISO, and other styles

38

Winkless, Laurie. "High-throughput screening platform for CRISPR." Materials Today 19, no. 3 (2016): 132. http://dx.doi.org/10.1016/j.mattod.2016.02.017.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Salzman, Sony. "How CRISPR Is Revolutionizing Screening Technology." Genetic Engineering & Biotechnology News 39, no. 4 (2019): S16—S18. http://dx.doi.org/10.1089/gen.39.04.23.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Salzman, Sony. "How CRISPR Is Revolutionizing Screening Technology." Genetic Engineering & Biotechnology News 39, S2 (2019): S16—S18. http://dx.doi.org/10.1089/gen.39.s2.06.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Zhang, Linfeng. "Innovative CRISPR-Cas9 enhances cell rejuvenation, addressing disease." Theoretical and Natural Science 91, no. 1 (2025): 89–96. https://doi.org/10.54254/2753-8818/2025.gu21568.

Full text

Abstract:

CRISPR-Cas9 technology, a groundbreaking gene editing tool, has shown promise in various fields such as genome modification, gene expression regulation, and functional screening. Despite its potential, the mechanisms by which CRISPR-Cas9 regulates cell fate and reprogramming remain incompletely understood, hampering its broad application in regenerative medicine and the treatment of age-related diseases. To address this, this study systematically reviews recent advancements in CRISPR-Cas9 and CRISPRa systems, focusing on their roles in cellular reprogramming, fate selection, and age-related di

APA, Harvard, Vancouver, ISO, and other styles

42

Chen, Sitong, Lin Yang, and Wei Li. "CRISPR Screening “Big Data” Informs Novel Therapeutic Solutions." CRISPR Journal 2, no. 3 (2019): 152–54. http://dx.doi.org/10.1089/crispr.2019.29062.sch.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Raffeiner, Philipp, Jonathan R. Hart, Daniel García-Caballero, Liron Bar-Peled, Marc S. Weinberg, and Peter K. Vogt. "An MXD1-derived repressor peptide identifies noncoding mediators of MYC-driven cell proliferation." Proceedings of the National Academy of Sciences 117, no. 12 (2020): 6571–79. http://dx.doi.org/10.1073/pnas.1921786117.

Full text

Abstract:

MYC controls the transcription of large numbers of long noncoding RNAs (lncRNAs). Since MYC is a ubiquitous oncoprotein, some of these lncRNAs probably play a significant role in cancer. We applied CRISPR interference (CRISPRi) to the identification of MYC-regulated lncRNAs that are required for MYC-driven cell proliferation in the P493-6 and RAMOS human lymphoid cell lines. We identified 320 noncoding loci that play positive roles in cell growth. Transcriptional repression of any one of these lncRNAs reduces the proliferative capacity of the cells. Selected hits were validated by RT-qPCR and

APA, Harvard, Vancouver, ISO, and other styles

44

Ganesh, Irisappan, Ilangovan Karthiga, Manoranjani Murugan, Kumar Rangarajalu, Vishnu Bhat Ballambattu, and Sambandam Ravikumar. "CRISPR/Cas-Based Prenatal Screening for Aneuploidy: Challenges and Opportunities for Early Diagnosis." Medicina 61, no. 4 (2025): 610. https://doi.org/10.3390/medicina61040610.

Full text

Abstract:

Aneuploidy is increasingly recognized globally as a common cause of miscarriage among expectant mothers. The existing prenatal screening techniques for detecting aneuploidy have several limitations. The ability to diagnose aneuploidy early in a non-invasive manner is not feasible with the current screening methods, as they may produce false positive or false negative results. Recently, the widely used gene editing tool CRISPR/Cas has shown great promise in diagnostics. This review summarizes the prenatal screening tests used in the first trimester to assess aneuploidy conditions. Additionally,

APA, Harvard, Vancouver, ISO, and other styles

45

Chulanov, Vladimir, Anastasiya Kostyusheva, Sergey Brezgin, et al. "CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions." Viruses 13, no. 11 (2021): 2258. http://dx.doi.org/10.3390/v13112258.

Full text

Abstract:

CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identification of novel drug candidates for treating viral infections. In this review, we highlight recent developments in the fields of CRISPR/Cas with a focus on the use of CRISPR screens for studying viral infections and identifying new candidate genes to aid development of antivirals.

APA, Harvard, Vancouver, ISO, and other styles

46

Liu, Zhuoxin. "CRISPR/Cas9 high-throughput screening in cancer research." E3S Web of Conferences 185 (2020): 03032. http://dx.doi.org/10.1051/e3sconf/202018503032.

Full text

Abstract:

In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely used in various models, including cell lines of mice and zebrafish. The use of CRISPR system to construct a genomic library for high-throughput screening is the main strategy for research of disease, especially tumor target gene research. This article reviews the basic principles and latest developments of CRISPR/Cas9 library screening technology

APA, Harvard, Vancouver, ISO, and other styles

47

Thege, Fredrik Ivar, Dhwani N. Rupani, Bhargavi B. Barathi, Anirban Maitra, Andrew D. Rhim, and Sonja M. Wörmann. "Abstract 918: Development of a platform for programmable in vivo oncogene activation and screening using CRISPRa technology." Cancer Research 82, no. 12_Supplement (2022): 918. http://dx.doi.org/10.1158/1538-7445.am2022-918.

Full text

Abstract:

Abstract Conventional genetically engineered mouse models (GEMMs) are time consuming, laborious and offer limited spatio-temporal control. We have developed a streamlined platform for in vivo gene activation using CRISPR activation (CRISPRa) technology. Our model system allows for flexible, sustained and timed activation of one or more target genes, in vitro or in vivo, using single or pooled lentiviral guides. Using Myc and Yap1 as model oncogenes, we implemented this platform to study the effect of oncogene activation on the tumorigenic potential of primary pancreatic organoids, as well as i

APA, Harvard, Vancouver, ISO, and other styles

48

Hajek, Roman, Tomas Jelinek, David Žihala, et al. "Deubiquitinase BAP1 is crucial for surface expression of T cell receptor (TCR) complex, T cell-B cell conjugate formation, and T cell activation." Journal of Leukocyte Biology 117, no. 1 (2024): qiae184. https://doi.org/10.1093/jleuko/qiae184.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Ramaker, Ryne C., Andrew A. Hardigan, Emily R. Gordon, Carter A. Wright, Richard M. Myers, and Sara J. Cooper. "Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition." BMC Cancer 21, no. 1 (2021). http://dx.doi.org/10.1186/s12885-021-08388-1.

Full text

Abstract:

Abstract Background Pancreatic ductal adenocarcinoma (PDAC) patients suffer poor outcomes, including a five-year survival of below 10%. Poor outcomes result in part from therapeutic resistance that limits the impact of cytotoxic first-line therapy. Novel therapeutic approaches are needed, but currently no targeted therapies exist to treat PDAC. Methods To assess cellular resistance mechanisms common to four cytotoxic chemotherapies (gemcitabine, 5-fluorouracil, irinotecan, and oxaliplatin) used to treat PDAC patients, we performed four genome-wide CRISPR activation (CRISPRact) and CRISPR knock

APA, Harvard, Vancouver, ISO, and other styles

50

Hu, Shijie, Mailin Gan, Ziang Wei, et al. "Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system." Frontiers in Microbiology 15 (November 21, 2024). http://dx.doi.org/10.3389/fmicb.2024.1498641.

Full text

Abstract:

Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered the third generation of gene editing after zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), is widely used for screening various viral host factors. CRISPR libraries are classified into three main categories based on the different functions of Cas9 enzymes: CRISPR knockout (CRISPR KO) library screening,

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!