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Basu, Roy Sayantani. "Répartition des crossovers méiotiques : hétérogénéité, modélisation de l'interférence, interaction entre voies de formation." Phd thesis, Université Paris Sud - Paris XI, 2014. http://tel.archives-ouvertes.fr/tel-00989127.
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Dans la plupart des organismes, les crossovers se formant au cours de la méiose sont interférérents : deux crossovers sont rarement à proximité. Nous avons étudié ce phénomène en détail dans la plante modèle Arabidopsis thaliana en utilisant une grande population de rétro-croisements, en méiose mâle et en méiose femelle. Nous avons utilisé le modèle Gamma à deux voies, superposant à la voie interférente une proportion p de crossovers d'une deuxième voie non interférente. La méiose femelle montre une interférence plus élevée et une proportion p plus faible que la méiose mâle. Par ailleurs nos comparaisons intra-chromosomiques concluent qu'il existe des variations d'interférence entre le bras gauche et le bras droit d'une part et entre la partie centrale et les régions distales d'autre part. Nous avons ensuite développé des tests statistiques qui ont révélé des hétérogénéités dans la proportion p le long des chromosomes. De telles variations ont ensuite été trouvées dans un jeu de données de tomate où notre analyse statistique nous a permis de montrer pour la première fois sur un organisme "sauvage" que la deuxième voie était très peu interférente et qu'en fait les deux voies avaient un peu de " cross-talk ". Vu que la limitation la plus sévère des modèles utilisés jusqu'à présent est l'hypothèse de constance du paramètre p, nous avons développé un modèle généralisé où les deux voies peuvent avoir des paysages de recombinaison différents. Ce modèle nommé PSL-GS a été implémenté en logiciel et testé sur des données simulées et sur trois jeux de données de plantes. L'utilisation du critère BIC suggère que tout comme chez la tomate, les paysages de recombinaison d'Arabidopsis et du maïs sont différents entre les 2 voies.
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Kanter, Jaimie. "Fan Fiction Crossovers| Artifacts of a Reader." Thesis, Hofstra University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10286513.
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Over twenty-five years ago, Henry Jenkins (1992) wrote that fan fiction writing is evidence of “exceptional reading” (p. 284) in that the fan text reflects a reader’s commentary. This investigation examined the ways in which crossover fan fiction, fan-written fiction that mixes elements of two or more well-known fictional worlds, might reveal evidence of this “exceptional reading.” Using a qualitative content analysis of 5 crossover texts that remix Rowling’s Harry Potter series and Austen’s Pride and Prejudice, the study focused on fan writers-as-readers of the source texts. Drawing on Rosenblatt’s (1988) transactional theory of reading, which posits that meaning resides in the transactions between reader, text, and writer, and that the meaning produced is a “new event,” this research concluded that the fan fiction writers’ crossover texts were, in part, a written record of some of the fan writers’ transactions with the source texts, a partial record of the “new event.” Furthermore, this analysis provided evidence that these fan readers-turned-writers demonstrated a powerful understanding of their intended and anticipated audience, a commanding and controlled use of emulation, and a calculated mingling of worlds—both to sustain and to disrupt the fan canon—in order to present their own interpretations of, comments on, and admiration for the source texts. The crossovers are evidence of “exceptional reading” in that they demonstrate the fan writers’ reading transactions.
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Kimene, Kaya Boniface Dimitri Christel. "Crossovers and phase transitions in Bose-Fermi mixtures." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86471.
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Thesis (MSc)--Stellenbosch University, 2014.ENGLISH ABSTRACT: We present a theoretical approach that allows for the description of trapped Bose-Fermi mixtures with a tunable interspecies interaction in the vicinity of a Feshbach resonance magnetic field.The many-body physics of the system is treated at equilibrium using the well-established mean-field and local density approximations. This reduces the physics locally to that of a homogeneous system. We observe a rich local phase structure exhibiting both first and second order phase transitions between the normal and BEC phases. We also consider the global properties of the mixture at a fixed number of particles and investigate how the density profiles and the populations of the various particle species depend on the detuning and trap profile.
AFRIKAANSE OPSOMMING: Ons beskou ’n teoretiese beskrywing van gevangde Bose-Fermi mengsels met ’n verstelbare interspesie wisselwerking in die teenwoordigheid van ’n magneties-geïnduseerde Feshbach resonansie. Die veeldeeltjiefisika van die sisteem word by ekwilibrium binne die welbekende gemiddelde-veld en lokale-digtheid benaderings hanteer. Sodoende word die fisika lokaal tot die van ’n homogene sisteem gereduseer. Ons neem ’n ryk fase-struktuur waar met beide eerste- en tweede-orde fase-oorgange tussen die normale en BEK fases. Ons beskou ook die globale eienskappe van die mengsel by ’n vaste totale aantal deeltjies en ondersoek hoe die digtheidsprofiele en deeltjiegetalle van die afstemming en die profiel van die val afhang.
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Barbera, Maria Antonia Petes Thomas D. "Selection and analysis of mitotic crossovers in Saccharomyces cerevisiae." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,959.
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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.Title from electronic title page (viewed Dec. 18, 2007). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Curriculum in Genetics and Molecular Biology." Discipline: Genetics and Molecular Biology; Department/School: Medicine.
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Alves, Sidiney Geraldo. "Leis de escala e Crossovers em modelos de crescimento." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/IACO-6WLS8K.
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Nesta tese de doutoramento, estudamos leis de escalas e Crossovers em modelos de crescimento de agregados e interfaces. Nossa abordagem consiste em uma analise de modelos discretos por meio de simulações com o objetivo de investigar suas classes de universalidade e o aparecimento de crossovers. Na primeira parte da tese, analisamos as propriedades de escala em interfaces. O primeiro modelo de crescimento de interfaces estudado foi construído para capturar os efeitos da competição entre a deposição e difusão de partículas. Encontramos uma transição no comportamento da rugosidade, que exibe um expoente de crescimento referente à classe de universalidade de Mullins-Hering (MH) para tempos curtos e à classe de Villain-Lai-Das Sarma (VLDS) para tempos longos. Em seguida, investigamos o modelo de Wolf-Villain com uma modificação na regra de crescimento introduzida para estudar crossovers. Concluímos esta parte da tese analisando estruturas com simetria radial, com atenção particular a detalhes associados à estratégia de medida da rugosidade que podem levar a conclusões equivocadas dos expoentes e, conseqüentemente, da classe de universalidade dos sistemas consederados. Na parte seguinte, consideramos transições morfológicas em modelos de agregação de partículas. Estudamos generalizações do modelo de agregação limitada por difusão (DLA). Na primeira, consideramos o efeito de uma tendência nas trajetórias das partículas. Mostramos que os agregados apresentam uma transição morfológica entre o modelo DLA e o modelo de agregação balística (BA) e caracterizamos quantitativamente essa transição por meio do comprimento característico. Em seguida, estudamos a anisotropia em agregados obtidos no modelo DLA através de uma generalização que inclui uma probabilidade de crescimento às regras de evolução e da aplicação da técnica de redução de ruído. Esse modelo apresenta um rico comportamento quando variamos os parâmetros envolvidos. Finalizamos essa tese com um modelo de agregação o qual consiste de uma mistura de partículas que executam caminhadas aleatórias e trajetórias balísticas. Neste modelo, encontramos uma transição suave de agregados semelhantes àqueles do modelo DLA para os do BA. No outro resultado está no campo dos modelos computacionais híbridos. Em particular, o Computador Semi-quântico. Nós investigamos o desempenho dos algoritmos de Busca e de Transformada de Fourier sobre esta arquitetura híbrida, constituída de processadores clássicos e quânticos trabalhando em conjunto. Mostramos que este tipo de modelo, o Semi-quântico, pode ser melhor que uma arquitetura puramente clássica, mesmo que trabalhando com poucos qubits, o que, por outro lado, pode ser mais fácil para uma implementação com a tecnologia corrente.
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Boddapati, Pavani. "Comparative study of type 2 crossovers and median u-turns." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/5676.
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Thesis (M.S.)--University of Missouri-Columbia, 2008.The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on August 10, 2009) Includes bibliographical references.
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Frezza, Giulia. "The concept of interaction : crossovers among biology, logic and philosophy." Paris 7, 2011. http://www.theses.fr/2011PA070008.
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Mon travail propose l'histoire épistémologique du concept d'interaction, que j'ai appelée son "hidden epistemological exaptation", à partir des domaines de la physique (complementarity principle) et de la psychologie (Gestalttheorie) en arrivant à la biologie contemporaine. Ma thèse est que, de ce point de vue, l'interaction est un processus doté d'une polarité. Il a un coté positif, du point de vue de l'action coordonnée entre deux dynamiques ou processus, mais, d'autre part, il a un coté négatif, en tant qu’inter-férence (littéralement: inter-fero en Latin, amener) qui résulte de la co-constitution dans le développement du processus même. Deuxièmement, je propose une comparaison entre ces analyses sur l'interaction et celles qui ont été achevées par l'approche géométrique de Jean-Yves Girard en logique linéaire et en Géométrie de l'Interaction (GdI) qui portent notamment à considérer le lien étroit entre l'interaction et la dualité en logique. L'analyse de l'usage et de la diffusion du ternie "interaction" dans les différents domaines montre une croissance intensive et extensive, notamment dans les 40 dernières années. J'en conclus que le concept d'interaction constitue une "rupture épistémologique" qui met en lumière un cadre épistémologique très expressif pour décrire les être vivants d'un point de vue théoriqueMy work discusses a possible epistemological history of the concept of interaction, which outlines what I name its hidden epistemological exaptation, from the domain of physics (complementarity principle) and psychology (Gestalttheorie) to its recent developments in biology. I advocate that, from this point of view, the interaction results a process due of a polarity. It has a "positive pole", being the coordinated action between two dynamics, or processes. At the same time it has a "negative pole" being an inter-ference (literally: inter-fero from Latin, to bear), in the sense of a result of the co-constitution in the development of the actual process. Moreover I stress a parallel between the discussed investigations about interaction and those achieved by Girard's geometric approach in linear logic and "Geometry of Interaction" (Gol). I especially point out the link between the notion of interaction and that of duality in logic. The analysis of the use and diffusion of the term "interaction" in various scientifïc disciplines shows an intense and extensive growth especially within the last forty years. I propose that we are assisting to a proper epistemological breaking which indicates that the concept of interaction has become now a precious epistemological framework for describing living phenomena from a theoretical point of view
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Fernandes, Joiselle Blanche. "Identification et caractérisation fonctionnelle de gènes contrôlant la fréquence de crossovers méiotiques." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS303/document.
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Les crossing-overs (CO) sont issus d’échange réciproque de matériel génétique entre les chromosomes homologues. Les COs produisent de la diversité génétique et sont essentiels chez la plupart des eucaryotes, pour la distribution équilibrée des chromosomes lors de la méiose. Malgré leur importance, et un large excès de précurseurs moléculaires, le nombre de CO est très limité dans la grande majorité des espèces (Typiquement 1 à 4 par paire de chromosomes). Cela suggère que les COs sont étroitement régulés, mais les mécanismes sous-jacents sont mal connus. Pour identifier les gènes qui limitent la formation des CO, l’équipe a mené un crible génétique chez Arabidopsis thaliana. Ces travaux ont mené à l’identification de plusieurs facteurs anti-CO, définissant trois voies : (i) L’hélicase FANCM et ses co-facteurs ; (ii) L’AAA-ATPase FIDGETIN-LIKE-1 (FIGL1) ; (iii) Le complexe RECQ4-Topoisomerase 3α-RMI1.Le premier objectif de ma thèse est d’explorer les relations entre ces trois voies en s’attachant aux questions suivantes ; (1) Jusqu’où peut-on augmenter la recombinaison en combinant les mutations dans FANCM, FIGL1 et RECQ4 ? Nous avons montré que la plus forte augmentation de recombinaison était obtenue dans recq4 figl1, atteignant 7,5 fois la fréquence du sauvage en moyenne sur le génome. (2) Quel est la distribution de ces extra-COs ? L’augmentation de recombinaison n’est pas homogène le long du génome : Les fréquences de CO augmente fortement des centromères vers les télomères, avec les plus hautes fréquences observées dans les régions distales. (3) La modification des fréquences de recombinaison est-elle identique lors des méioses mâles et femelle ? Chez le sauvage, la fréquence de recombinaison est plus élevée lors de la méiose mâle que femelle. Au contraire, la recombinaison femelle devient plus élevée que la recombinaison mâle chez les mutants recq4 et recq4 figl1. Ceci suggère que des contraintes qui s’appliquent sur la formation des CO lors de la méiose femelle sont relâchées chez ces mutants. En poursuivant le crible génétique, un nouveau mutant hyper-recombinant a été identifié. Le second objectif de ma thèse fut d’identifier et de caractériser fonctionnellement le gène correspondant. Une cartographie génétique et des études d’interactions protéine-protéine, ont mené à l’identification d’un facteur qui interagit directement avec FIGL1 et semble former un complexe conservé depuis les plantes jusqu’au mammifères. Nous avons baptisé cette protéine FLIP (Fidgetin-like-1 interacting protein). Les fréquences de recombinaisons sont augmentées dans flip-1, confirmant que FLIP1 limite la formation des COs. Des études d’épistasie ont montré que FLIP et FIGL1 agissent dans la même voie. De plus les protéines FIGL1/FLIP d’Arabidopsis ou humaine, interagissent avec RAD51 et DMC1, les deux protéines qui catalyse une étape clef de la recombinaison, l’invasion d’un ADN homologue. Finalement, dans flip comme dans figl1, la dynamique de DMC1 est modifiée. Nous proposons donc un modèle dans lequel le complexe FLIP-FIGL1 régule négativement l’activité de RAD51/DMC1 pour limiter la formation des COs. L’étude du complexe conservé FLIP-FIGL1 a mis en évidence un nouveau mode de régulation de la recombinaison, qui agit vraisemblablement à l’étape clé de l’échange de brin homologue. De plus, l’augmentation des CO sans précédent obtenues chez recq4 figl1 peut être d’un grand intérêt pour l’amélioration des plantes en permettant de diversité de nouvelles combinaisons alléliquesMeiotic crossovers (CO) are formed by reciprocal exchange of genetic material between the homologous chromosomes. CO generate genetic diversity and are essential for the proper segregation of chromosomes during meiosis in most eukaryotes. Despite their significance and a large excess of CO precursors, CO number is very low in vast majority of species (typically one to three per chromosome pair). This indicates that COs are tightly regulated but the underlying mechanisms of this limit remain elusive. In order to identify genes that limit COs, a genetic screen was performed in Arabidopsis thaliana. This led to the identification and characterization of several anti-CO factors belonging to three different pathways: (i) The FANCM helicase and its cofactors (ii) The AAA-ATPase FIDGETIN-LIKE-1 (FIGL1) (iii) The RECQ4 -Topoisomerase 3α-RMI1 complex. The first objective was to understand the functional relationship between these three pathways and to address following questions: (1) how far can we increase recombination when combining mutations in FANCM, FIGL1 and RECQ4? We show that the highest increase in recombination was obtained in figl1 recq4, reaching to 7.5 fold the wild type level, on average genome wide. (2) How is the distribution of recombination events genome wide in mutants? The increased CO frequency in the mutants was not uniform throughout the genome. CO frequency rises from the centromere to telomeres, with distal intervals having highest COs (3) is the recombination frequency increase same in both male and female? In Arabidopsis wild type, male has higher recombination than female meiosis. In contrast, in recq4 and recq4 figl1, female recombination was higher than male. This suggests that certain constraints that apply to CO formation in wild type females are relieved in the mutant. By continuing the same genetic screen, a novel anti-CO mutant was identified. The second objective was to identify and functionally characterize the corresponding gene. Genetic mapping and protein interaction studies led to the identification of a factor that directly interacts with FIGL1 and appears to form a conserved complex both in Arabidopsis and humans. Hence, the factor was named FLIP (Fidgetin-like-1 interacting protein). Recombination frequency is increased in flip, confirming that FLIP limit COs. Epistasis studies showed that FLIP and FIGL1 act in same pathway. Further, FIGL1/FLIP proteins of Arabidopsis and humans directly interact with the recombinases RAD51 and DMC1 which catalyze a crucial step of homologous recombination, the inter homolog strand invasion. In addition flip like figl1 modifies dynamics of DMC1. We thus propose a model wherein the FLIP-FIGL1 complex negatively regulates RAD51/DMC1 to limit CO formation. Studying the conserved FIGL1-FLIP complex led to the identification of a novel mode of regulation of recombination, that likely acts at the key step of homologous strand invasion. Further the unprecedented level of CO increase in recq4figl1 in hybrids could be of great interest for crop improvement, allowing the production of novel allele combinations
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Akula, Mohan Kumar. "Guidelines for safety and design improvements at rural expressway median crossovers /." free to MU campus, to others for purchase, 2003. http://wwwlib.umi.com/cr/mo/fullcit?p1417999.
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Sandhu, Amritpal Singh. "Manipulating the frequency and distribution of genetic crossovers during meiosis in barley." Thesis, University of Birmingham, 2015. http://etheses.bham.ac.uk//id/eprint/6316/.
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In commercial barley cultivars meiotic crossover (CO) distribution is skewed to the distal regions of the paired chromosomes. This restricts recombination to these regions thereby reducing the potential genetic variation that can be exploited in plant breeding programs. The aim of this project was to develop experimental strategies that will enable the frequency and distribution of meiotic crossovers to be modified in order to generate progeny with novel gene combinations. Treatment with the histone deacetylase inhibitor trichostatin A, led to significant modifications in crossover frequency in a concentration-dependent manner with lower concentrations not greatly impacting fertility, allowing for the extraction of fertile seeds. The genetic screening of a treated marker population at The James Hutton Institute (JHI), demonstrated subtle but significant shifts in the distribution of meiotic recombination, indicating that modifying recombination through chemicals applied via the transpiration stream is indeed feasible in barley and hence, possibly in other cereals. The cytological study of a barley desynpatic mutant \(des8\) in collaboration with JHI revealed that synapsis is normal despite reduced chiasma frequency. Genetic mapping studies are in progress to identify the mutant gene responsible for this phenotype which will help us to improve our current knowledge of meiosis in barley.
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Reimann, Thomas. "Resonant spin dynamics and 3D-1D dimensional crossovers in ultracold Fermi gases." Thesis, Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLEE029/document.
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L’exploration de systèmes quantiques à N corps fortement corrélés représente l’un des domaines de recherche les plus stimulants de la physique contemporaine. Au cours des trente dernières années, les vapeurs diluées d’atomes neutres en suspension dans le vide et contrôlées par un laser sont devenues une plate-forme polyvalente et formidable pour l’étude de tels systèmes. L’intérêt principal réside dans la capacité d’ajuster arbitrairement la force de l’interaction atomique au moyen de résonances de Feshbach induites magnétiquement, ainsi que la possibilité de créer une large gamme de potentiels via des champs optiques précisément adaptés. Cette thèse présente les résultats récents de l’expérience FerMix, consacrée à l’étude des systèmes quantiques à plusieurs corps fermioniques à des températures ultra-basses utilisant les atomes alcalins 40K et 6Li. Les principaux résultats présentés dans ce texte sont doubles. Premièrement, nous rapportons la caractérisation expérimentale d’une nouvelle résonance de Feshbach (s,d)-wave du 40K, dont les résultats sont comparés aux prédictions théoriques correspondantes. En particulier, le spectre du taux de perte inélastique est déterminé pour différentes températures et profondeurs de piège, ce qui nous permet d’identifier les pertes en tant que processus à deux corps. De plus, il est confirmé que le canal d’entrée dominant est de type s-wave. À l’aide de modèles d’équation de taux, nous analysons le réchauffement observé de l’ensemble atomique et trouvons que le comportement est cohérent avec l’état lié prévu L = 2 présent dans le canal de sortie. Enfin, nous étudions expérimentalement la dynamique des populations de spin induite par les collisions inélastiques renforcées par résonance dans l’onde d, en observant un bon accord avec nos modèles numériques. En second lieu, nous résumons nos progrès dans l’étude des croisements dimensionnels entre le liquide de Tomonaga-Luttinger en 1D et le liquide de Landau-Fermi en 3D en utilisant les gaz de Fermi de 40K confinés dans un réseau optique à grand pas. Cela inclut à la fois les considérations de conception fondamentales et l’installation du matériel expérimental requisThe exploration of strongly correlated quantum many-body systems represents one of the most challenging fields of research of contemporary physics. Over the past thirty years, dilute vapors of neutral atoms suspended in vacuum and controlled with laser light have become a versatile and powerful platform for the study of such systems. At the very heart lies the ability to arbitrarily tune the interaction strength by means of magnetically induced Feshbach resonances as well as the possibility to create a wide range of potential landscapes via precisely tailored optical fields. This thesis reports on the recent results of the FerMix experiment, which is dedicated to the study of fermionic quantum many-body-systems at ultralow temperatures using the Alkali atoms 40K and 6Li. The main results presented in this text are twofold. First, we report on the experimental characterization of a novel (s,d)-wave Feshbach resonance in 6Li, the results of which are compared to the corresponding theoretical predictions. In particular, the spectrum of the inelastic loss rate is determined for different temperatures and trap depths, which enables us to identify the losses as two-body processes. Moreover, the dominant entrance channel is confirmed to be s-wave in nature. Using rate equation models we analyze the observed heating of the atomic ensemble and find the behavior to be consistent with the predicted L = 2 bound state present in the exit channel. Finally, we investigate experimentally the dynamics of the spin populations driven by resonantly enhanced inelastic collisions in dwave, observing good agreement with our numerical models. Second, we summarize our progress towards the study of dimensional crossovers between the Tomonaga-Luttinger liquid in 1D and the Landau-Fermi liquid in 3D using Fermi gases of 40K confined in a large spacing optical lattice. This includes both the fundamental design considerations as well as the implementation of the required experimental hardware
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Gonzalo, Adrian. "Voies de formation des crossovers méiotiques chez une espèce allopolyploïde, le colza (Brassica napus)." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS352.
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La recombinaison méiotique est au cœur de l'hérédité Mendélienne, de l'évolution et de l'amélioration des plantes, car elle assure, grâce aux crossovers, une transmission fidèle des chromosomes et le brassage de l’information génétique au fil des générations. Deux voies de formation des crossovers coexistent chez les plantes. La voie principale (voie I) dépend de la protéine MSH4 (et de quelques autres). La voie secondaire ne produit que quelques crossovers (dits de voie II) au cours de la méiose d’une plante de type sauvage ; ils sont indépendants de MSH4 et leur nombre est limité par des protéines telles que FANCM. Si ces deux voies ont été bien décrites chez des espèces diploïdes, ce n’est pas le cas chez des plantes allopolyploïdes, pourtant très fréquentes parmi les plantes cultivées. Il s'agit là d'une lacune importante, car la présence de plusieurs jeux de chromosomes apparentés conduit à augmenter le nombre de partenaires susceptibles de former des crossovers et le nombre de copies de tous les gènes méiotiques, rendant la recombinaison méiotique plus complexe. Cette thèse vise à explorer l'interaction entre les voies de formation des crossovers et la polyploïdie en utilisant des mutants de colza (Brassica napus; AACC) et d’un de ces parents diploïdes (B. rapa; AA) pour deux gènes de la recombinaison méiotique.J'ai tout d'abord testé dans quelle mesure la formation de crossovers entre chromosomes homologues et entre homéologues (chromosomes A et C) est tributaire des voies I et II en évaluant l’effet d’une diminution du nombre de copies fonctionnelles de MSH4 sur le nombre de crossovers. J'ai montré que ce dernier n'est altéré que lorsque les deux copies MSH4 sont inactivées, toute autre combinaison de mutations conduisant au même nombre de crossovers inter-homologues que chez le sauvage. J'ai également montré que la proportion de crossovers de voie II chez des mutants msh4 de colza est bien supérieure à celles observées chez d’autres plantes mutantes pour msh4. Cette observation reste vraie chez des mutants msh4 de B. rapa, suggérant que la proportion accrue de crossovers de voie II n’est pas spécifique au colza, mais probablement une caractéristique des Brassicaceae. Chez des plantes allohaploïdes (AC) de colza, chez lesquelles les crossovers ne peuvent se former qu’entre homéologues, les copies MSH4 ne se compensent plus complétement ; le nombre de crossovers de voie I fluctue au contraire proportionnellement au dosage de MSH4, devenant presque nul lorsque toutes les copies sont inactivées. Mes résultats illustrent deux nouvelles propriétés spécifiques des crossovers entre homéologues: une plus grande sensibilité vis-à-vis du dosage MSH4 pour les crossovers de voie I et une plus faible efficacité des crossovers de voie II.Dans un second temps, j'ai caractérisé cytologiquement des mutants fancm de colza pour vérifier que l'augmentation des crossovers de voie II ne nuit pas à au bon déroulement de sa méiose. Cette question est restée en suspens, les mutants fancm de colza n’étant pas complètement nuls. Cet écueil m'a incité à développer une approche de TILLING par séquençage pour identifier de nouveaux mutants de recombinaison chez le colza. J'ai alors combiné les mutations fancm et msh4 chez B. rapa pour vérifier si les premières suffisent à corriger les défauts méiotiques induits par les secondes. J'ai montré que, conformément à ce qui avait été observé chez Arabidopsis thaliana, la mutation fancm augmente le nombre de crossovers à un point tel qu’elle restaure la formation de bivalents dans un mutant msh4. La fonction de FANCM est donc conservée chez B. rapa.Mes résultats ont fait progresser la compréhension des voies de formation des crossovers lors d’une méiose allopolyploïde. Ils indiquent que la transmission des chromosomes chez ces espèces implique principalement des crossovers de voie I, et qu’elle pourrait être assurée en limitant l’efficacité de cette voie (e.g. en diminuant le nombre de copies de gènes)Meiotic recombination ensures, through the formation of crossovers (COs), both faithful chromosome transmission and allelic shuffling over generations; it is at the heart of Mendelian heredity, evolution and plant breeding. Two crossover pathways co-exist in plants. The main pathway (class I) is dependent on MSH4 (and additional proteins). The secondary pathway produces only a few MSH4-independent (class II) crossovers during wild-type meiosis that are limited in number by anti-crossover proteins such as FANCM. These pathways have been extensively described in diploid species, disregarding one of the most pervasive features of crop genomes: polyploidy. This is a major gap in our understanding because the presence of more than two related sets of chromosomes leads both to extra partners for crossover formation and additional copies for all meiotic genes, which make meiotic recombination more intricate. This thesis aims at exploring the interplay between meiotic recombination pathways and polyploidy using mutants for two recombination genes in allotetraploid Brassica napus (AACC; 2n=38) and its diploid progenitor, B. rapa (AA; 2n=20). I have first tested the extent to which class I and class II pathways contribute to inter-homolog and inter-homoeolog (between A and C chromosomes) crossover formation by analyzing how crossovers are affected as the number of functional MSH4 copies decreases. I showed that inter-homolog crossover formation is impaired only when the two MSH4 copies are lost, any other combination of msh4 mutations resulting in wild-type crossover numbers. I also observed that, when class I crossovers are completely abolished in B. napus, the highest frequency of class II crossover ever reported among plant msh4 mutants is observed. I reproduced this result using B. rapa msh4 mutants, thereby demonstrating that increased class II crossover frequencies is not specific to B.napus, but could instead be a general feature of the Brassicaceae. In B. napus allohaploids (AC), where crossovers are forced to occur between homeologs, MSH4 copies no longer complement each other perfectly; counter to the situation in euploids, the number of MSH4-dependent crossovers formed between homoeologs fluctuates with MSH4 dosage in these plants, and approximate zero when all MSH4 copies are depleted. Altogether, my results illustrate two novel specific properties of inter-homeolog crossovers: a greater sensitivity to MSH4 dosage for class I pathway and a lower efficiency for class II.Next, I characterized cytologically B. napus fancm mutants to confirm that boosting class II crossovers would not be detrimental to B. napus meiosis. However, a prudential interpretation of these results is demanded since the B. napus fancm alleles retained residual anti-crossover activity. This has prompted me to set up a TILLING-by-sequencing procedure in order to produce new recombination mutants in B. napus. I also combined the B. rapa fancm and msh4 mutations to test whether the former is sufficient to fix the meiotic defects resulting from the latter. I showed that, similarly to what had been observed in Arabidopsis thaliana, fancm mutation boost COs to such a point that it restores bivalent formation in B. rapa msh4 background. My results therefore confirmed that the function of FANCM is conserved in B. rapa. Overall, the findings and achievements of this thesis make a step forward dissection of CO pathways during allopolyploid meiosis. They indicate that meiotic adaptation to allopolyploidy mainly involve the class I crossover pathway and could be achieved by limiting its efficiency (e.g. by decreasing gene copy number)
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Grandont, Laurie. "Caractérisation de variations naturelles de fréquence de crossovers chez le colza (Brassica napus)." Thesis, Paris 11, 2012. http://www.theses.fr/2012PA112034.
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La méiose est un processus fondamental qui conditionne la formation de gamètes et assure la stabilité des génomes tout en générant de la diversité par brassage génétique. La régularité méiotique nécessite la formation de crossing-overs (CO) exclusivement entre chromosomes homologues. Cette condition est plus difficile à remplir chez les espèces allopolyploïdes qui présentent plusieurs jeux de chromosomes toujours susceptibles de recombiner ensemble. Bien que la polyploïdie soit omniprésente chez les plantes, on connait peu de choses sur le déroulement de la méiose chez ces espèces. Au cours de ma thèse, je suis intéressée à l’effet de la polyploïdie sur la formation et sur la fréquence de CO en utilisant le colza (Brassica napus, AACC, 2n=38) comme modèle d’étude. J’ai notamment cherché à comprendre : (1) quel est l’effet du niveau de ploïdie sur la fréquence de crossovers, et (2) l’origine des variations de COs observées chez les plantes allohaploïdes (AC) produites à partir de différentes variétés de colza, en utilisant une palette d’approches cytologiques et cytogénétiques. Mes travaux ont permis de montrer que le niveau de ploïdie induit une augmentation de la fréquence de crossovers, et que cette augmentation est plus importante dans un contexte triploïde que tétraploïde. J’ai ainsi montré que la fréquence de CO augmente progressivement du diploïde (1,6 CO/bivalent) vers le tétraploïde (2 CO/bivalents) et quelle est maximale chez le triploïde (2,8 CO/bivalent). En ce qui concerne la deuxième question, j’ai montré que la différence entre les allohaploïdes de colzas apparaît tardivement au cours de la méiose. Elle semble être liée à une capacité différente à former des CO en fonction de la variété utilisée pour produire ces allohaploïdes et non pas à une différence dans la reconnaissance de l’homologie. Un de mes résultats original est que la protéine HEI10, impliquée dans la voie de formation des CO interférents, présente une dynamique différente entre les deux variétés, que ce soit à l’état euploïde (AACC) qu’allohaploïde (AC).Mes résultats conduisent à s’interroger sur la relation entre (i) la régulation du nombre de CO formés entre chromosomes homologues et (ii) la suppression des CO entre chromosomes non homologues chez les espèces allopolyploïdesMeiosis is a fundamental process required to produce gametes, ensure genome stability and generate diversity within species by creating new chromosome/allele combinations. For all these outcomes the exclusive formation of crossovers (CO) between homologous chromosomes is required. This condition is more difficult to fulfil in allopolyploid species that have more than two sets of chromosomes still able to recombine together. Although polyploidy has been particularly prevalent in plants, little is known about meiosis in polyploids. During my thesis I have analyzed the effect of polyploidy on CO formation and frequency, using oilseed rape (Brassica napus, AACC, 2n=38) as model. My work aimed to investigate (i) the effect of ploidy level on the rate of meiotic COs and (ii) the causes for the observed difference in CO rate between allohaploid plants (AC) produced from different B. napus varieties. To address these questions, I have combined a series of cytological, immunocytological and cytogenetical analyses.My work first indicates that polyploidization leads to increase CO frequency. I showed that the number of COs progressively increases from the diploid (1,6 CO/bivalent) to the tetraploid (2 CO/bivalent) and is maximal in the triploid (2,8 CO/bivalent). In the second part, I have shown that the difference of meiotic behaviors between B. napus allohaploids appears at a late stage of meiosis. This difference seems to be due to a difference in the propensity to form CO between the two varieties rather than a difference in the stringency of homology recognition. This difference could be related to the difference in the pattern and/or chronology of HEI10 (a key protein involved in the interfering CO pathway) signals along chromosomes during prophase I in both euploids (AACC) and allohaploids (AC).My results thus puts under the spotlight the link that may exist between (i) the regulation of CO rate between homologous chromosomes and (ii) the suppression of COs between non-homologous chromosomes in polyploid species
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Morden, Karen Michele. "The odd boy : Sixties crossovers and cultural explorations in the work of Vivian Stanshall." Thesis, University of Sussex, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414784.
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Galland, Lanie Maria. "Investigation of chromosome size effect on the rate of crossovers in the meiotic yeast Saccharomyces cerevisiae." DigitalCommons@CalPoly, 2014. https://digitalcommons.calpoly.edu/theses/1193.
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Meiosis is a specialized type of cell division characterized by a single round of DNA replication and two rounds of chromosome segregation, ultimately resulting in four haploid cells. During meiosis I, chromosomes align and reciprocal recombination results in the formation of a crossover, creating the tension required to properly segregate homologs during the first round of meiosis.
Two mechanisms involved in regulating the occurrence of crossing over are assurance and interference. Crossover assurance describes the phenomenon that at least one crossover will form between each pair of homologous chromosomes during prophase I. Crossover interference, on the other hand, describes the nonrandom placement of crossovers between homologs, increasing the probability that a second crossover will occur at a discrete distance away from the first one.
In addition to assurance and interference, chromosome size may play a role in the rate of meiotic recombination during prophase I. As a result of crossover assurance, small chromosomes receive a minimum of one crossover, the obligate crossover. Assuming chromosome size does not influence the rate of recombination, pairs of large chromosomes should experience the same number of crossovers per base pair as small chromosomes. Previous studies have been inconsistent: Kaback et al. (1999) saw decreased rates of crossing over between large chromosomes relative to small ones, suggesting that crossover interference acts across a larger distance on large chromosomes. Turney et al. (2004), however, saw no such effect, suggesting that these findings may be site- or sequence-specific.
The current study used the Cre-loxP system to create translocated chromosomes, decreasing the size of chromosome VIII from 562 kb to 125 kb. The rate of crossing over was evaluated using nutrient marker genes that were inserted on the left arm of chromosome VIII to facilitate phenotypic detection of crossing over between homologous translocated chromosomes in comparison to crossing over between homologous nontranslocated chromosomes.
Translocated strains were attempted, though further testing suggests that the translocation itself may be lethal. In the future, we plan to further investigate the potential lethal nature of the translocation.
We also experienced difficulty in curing yeast cells of the Cre expression plasmid: as pSH47 was removed, translocated chromosomes reverted to nontranslocated chromosomes. In addition, crossing over in nontranslocated yeast, along with subsequent molecular analysis, revealed that one of the marker genes presumed to be on the left arm of chromosome VIII is, in fact, located on a different chromosome, preventing analysis of crossing over in this region. As a result, we were unable to proceed with current experimentation.
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Carnochan, Stuart. "Orbit and altimetric corrections for the ERS satellites through analysis of single and dual satellite crossovers." Thesis, Aston University, 1996. http://publications.aston.ac.uk/14262/.
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Due to the failure of PRARE the orbital accuracy of ERS-1 is typically 10-15 cm radially as compared to 3-4cm for TOPEX/Poseidon. To gain the most from these simultaneous datasets it is necessary to improve the orbital accuracy of ERS-1 so that it is commensurate with that of TOPEX/Poseidon. For the integration of these two datasets it is also necessary to determine the altimeter and sea state biases for each of the satellites. Several models for the sea state bias of ERS-1 are considered by analysis of the ERS-1 single satellite crossovers. The model adopted consists of the sea state bias as a percentage of the significant wave height, namely 5.95%. The removal of ERS-1 orbit error and recovery of an ERS-1 - TOPEX/Poseidon relative bias are both achieved by analysis of dual crossover residuals. The gravitational field based radial orbit error is modelled by a finite Fourier expansion series with the dominant frequencies determined by analysis of the JGM-2 co-variance matrix. Periodic and secular terms to model the errors due to atmospheric density, solar radiation pressure and initial state vector mis-modelling are also solved for. Validation of the dataset unification consists of comparing the mean sea surface topographies and annual variabilities derived from both the corrected and uncorrected ERS-1 orbits with those derived from TOPEX/Poseidon. The global and regional geographically fixed/variable orbit errors are also analysed pre and post correction, and a significant reduction is noted. Finally the use of dual/single satellite crossovers and repeat pass data, for the calibration of ERS-2 with respect to ERS-1 and TOPEX/Poseidon is shown by calculating the ERS-1/2 sea state and relative biases.
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Trumper, Alex. "Comparison of skate boot pressure of elite and recreational hockey players during the performance of forward crossovers." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98507.
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The purpose of this paper was to characterize and compare pressure patterns in the skate boot of elite and recreational players during the forward crossover turn. In-skate pressure patterns of eight elite varsity level hockey players (mean +/- SD: height (m) = 1.80 +/- 0.07, weight (kg) = 87 +/- 0.06) and eight recreational hockey players (mean +/- SD: height = 1.76 (m) +/- 0.06, weight (kg) = 82 +/- 0.07) were measured using fifteen piezo-resistive sensors per foot during the forward crossover skating stride. Each participant performed three trials in both the clock-wise (CW) and counter clock-wise (CCW) directions. For each trial the pressure profiles of three strides were cut and averaged according to ability groups and turn directions. The results showed that the elite skaters performed the skill quicker than the recreational skaters (6.85 (sec) +/- 0.114 vs 7.62 (sec) +/- 0.125), respectively (p < 0.01). Other significant differences (p < 0.05) were found in peak pressures on the plantar, medial and lateral surfaces between groups. No significant differences were found when turn directions were compared. These results show that recreational skaters differ from elite skaters by displaying higher peak pressures on the plantar surface and lower peak pressures on the lateral and medial surfaces. Also, pressures on the plantar, medial and lateral surfaces appeared to be higher on the inside foot when compared to pressures on the outside foot for both groups.
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Girard, Chloé. "FANCM et ses cofacteurs MHF1-MHF2, ainsi que FIDGETIN-Like-1 limitent la formation des crossovers méiotiques chez Arabidopsis thaliana." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112259/document.
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La grand majorité des espèces forment très peu de crossovers (CO) par chromosome en méiose, et ce quelle que soit la taille des chromosomes et en dépit de l'excès de précurseurs de recombinaison disponibles. Les mécanismes qui sous-tendent cette limitation restent, pour une grande part, inconnus. Pour identifier des facteurs limitant la formation des CO en méiose, nous avons mis en place un crible permettant d'isoler des mutants formant plus de CO que le sauvage. Chez Arabidopsis thaliana, les mutants présentant un défaut de CO (ex: les mutants zmm) présentent une réduction de fertilité directement observable sur la longueur des fruits. Notre crible repose sur l'idée qu'une mutation augmentant la formation des CO devrait restaurer la fertilité des mutants zmm.Le premier suppresseur identifié nous a permis de montrer que FANCM est un régulateur majeur de la formation des CO méiotiques, en limitant la voie dépendante de MUS81, normalement minoritaire chez Arabidopsis (Crismani et al., 2012). Nous avons ensuite montré que deux des cofacteurs de FANCM au sein de la voie de l'anémie de Fanconi (FA), MHF1 et MHF2, agissent dans la même voie pour limiter les CO. En dehors de ces trois protéines, les autres protéines FA ne sont pas des protéines anti-CO (Girard et al., 2014).Nous avons ensuite identifié FIDGETIN-Like-1 comme une protéine anti-CO agissant en parallèle de FANCM. En effet, si les deux mutations figl1 et fancm alimentent la voie dépendante de MUS81 de formation des CO, leurs effets sont cumulatifs et mènent à une augmentation d'un facteur 6 du nombre CO par méiose, et ce sans affecter la ségrégation des chromosomes. Ce résultat montre que FIGL1 et FANCM agissent à deux étapes indépendantes de la recombinaison, et nos données suggèrent que FIGL1 pourrait agir en amont de FANCM.Ce travail a révélé l'existence d'au moins deux mécanismes de limitation des CO méiotiques, et montre que la fréquence de CO peut être largement augmentée sans que la ségrégation des chromosomes ne soit affectéeMost species only few meiotic crossovers (COs) per chromosome irrespective of their physical size and despite an excess of recombination precursors. However, the underlying mechanisms constraining CO frequency remain largely unknown. In order to find factors limiting meiotic COs, we performed a genetic screen to find mutants with increased CO frequency. CO-deficient mutants (e.g. zmm) of Arabidopsis thaliana display reduced fertility, easily noticeable by their obvious reduction in fruit length. We designed a screen based on the idea that mutations that increase CO frequency will restore the fertility of the zmm mutants.We showed first identified FANCM as a major anti-CO protein limiting MUS81-dependent COs, a normally minor pathway in Arabidopsis (Crismani et al., 2012). We then showed that two of FANCM's cofactors from the Fanconi anemia pathway of DNA repair, namely MHF1 and MHF2, act along FANCM to limit meiotic crossovers, whereas the other Fanconi proteins do not (Girard:2014).Another mutant revealed FIDGETIN-Like-1 (FIGL1) as an anti-CO factor that acts in parallel to FANCM. While both figl1 and fancm mutations fuels the MUS81-dependent CO pathway, the effect of both mutations is cumulative, leading to a six-fold increase in CO formation, without impairing chromosome segregation. This shows that FIGL1 and FANCM act independently, and our data suggest that FIGL1 could act at an earlier step in the recombination pathway than FANCM.This work reveals that at least two different mechanisms limit meiotic CO number and shows that CO frequency can be largely increased without affecting chromosome distribution at meiosis
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Reynolds, Matthew D. "The use of ERS-1 crossovers and Topex/Poseidon repeat pass data for global sea surface variability studies." Thesis, Aston University, 1998. http://publications.aston.ac.uk/13281/.
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In previous sea-surface variability studies, researchers have failed to utilise the full ERS-1 mission due to the varying orbital characteristics in each mission phase, and most have simply ignored the Ice and Geodetic phases. This project aims to introduce a technique which will allow the straightforward use of all orbital phases, regardless of orbit type. This technique is based upon single satellite crossovers. Unfortunately the ERS-1 orbital height is still poorly resolved (due to higher air drag and stronger gravitational effects) when compared with that of TOPEX/Poseidon (T/P), so to make best use of the ERS-1 crossover data corrections to the ERS-1 orbital heights are calculated by fitting a cubic-spline to dual-crossover residuals with T/P. This correction is validated by comparison of dual satellite crossovers with tide gauge data. The crossover processing technique is validated by comparing the extracted sea-surface variability information with that from T/P repeat pass data. The two data sets are then combined into a single consistent data set for analysis of sea-surface variability patterns. These patterns are simplified by the use of an empirical orthogonal function decomposition which breaks the signals into spatial modes which are then discussed separately. Further studies carried out on these data include an analysis of the characteristics of the annual signal, discussion of evidence for Rossby wave propagation on a global basis, and finally analysis of the evidence for global mean sea level rise.
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Kohzaki, Masaoki. "Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse." Kyoto University, 2007. http://hdl.handle.net/2433/135695.
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Robert-Lachaine, Xavier. "Force measurement and ankle motion of the forward skating and crossovers with a standard hockey skate and a modified hockey skate." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97222.
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Dynamic forces were measured during skating directly on-ice using a Bauer One95 and a second One95 with a modified tendon guard and eyelet configuration (DROM). The intent was to determine if mechanical differences exist in push-off force and ankle kinematics between the two skates. The right skate of each type was instrumented with a calibrated force transducer system to measure medial-lateral and vertical forces during ice skating. In addition, a goniometer was installed about the ankle to measure kinematics during skating. The ten subjects executed three skills: forward skating and forward crossovers in both directions. The DROM skate demonstrated significant gains in plantarflexion and net plantar-dorsiflexion ROM. In general this was not reflected in greater kinetic output except for greater medial-lateral forces. Total peak force occurred later during plantarflexion, suggesting that the increased ROM resulted in a more prolonged force generation during a given skating stride. The 14 to 20% increases in work and power output while wearing the DROM skates did not translate into improved times for these skating tasks. These apparently contradictory findings may well be attributed to lack of player familiarity with the modified skate's greater ankle mobility. Hence, to determine the DROM skate's true performance benefits, a longitudinal study of a cohort of players training with the DROM skate is required.Les forces de patinage ont été mesurées directement sur glace avec un Bauer One95 (Régulier) et un second One95 qui détenait un protecteur du tendon d'Achille modifié et une configuration différente des œillets pour lacets (DROM). Le but était de déterminer si des différences mécaniques existent dans la force de propulsion et la cinématique de la cheville entre les deux modèles. Le patin droit était instrumenté d'un système calibré d'estimation de la force qui permettait la mesure dynamique des forces durant le patinage. De plus, un goniomètre était installé autour de la cheville pour mesurer la cinématique durant le patinage. Les dix sujets accomplissaient le patinage avant et le croisé-avant dans les deux directions. Le patin DROM démontrait des gains significatifs en flexion plantaire et amplitude de mouvement frontale. En général, ces résultats ne se sont pas reflétés en bénéfices cinétiques, à l'exception des forces médio-latérales. La force totale maximale était délayée avec le DROM, suggérant une production de force prolongée qui résultait de l'amplitude de mouvement supplémentaire. Les augmentations de 14 à 20% en travail et puissance avec le DROM ne se sont pas traduites en amélioration de vitesse. Ces trouvailles apparemment contradictoires pourraient être attribuables au manque d'accoutumance des joueurs avec le patin modifié qui procure une mobilité supérieure à la cheville. Afin de déterminer le potentiel optimal du DROM sur la performance, une étude longitudinale avec un groupe de joueurs s'entraînant avec le DROM est nécessaire.
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Hüttl, Ondřej. "Nízkofrekvenční reprosoustava s ozvučnicí z alternativních materiálů." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217468.
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This thesis firstly consists of an analysis and a description of materials suitable for construction of loudspeaker enclosures and a design of the loudspeaker system with the enclosure made of a synthetic stone. Electro – acoustics drivers have been described with a focus to a frequency range, a principle and a construction for an explanation of their function. Drivers based on electrodynamic principle have been described closely. An analysis of materials used for the loudspeaker enclosure production has been done and tradicional and alternative materials have been described as well. Specific modulus, density and stiffness have been taken as an objective evaluative criterion. An evaluation indicates appropriate properties of plastic, aluminum and stone based materials. The function and kinds of loudspeaker enclosures have been described. The function, properties and design of a vented box loudspeaker system have been described closely. The last part of this thesis is individual design of a two-way vented box loudspeaker system with the enclosure made of synthetic stone. A simulation of a driver behavior built in the enclosure and compensation circuits and crossovers design and simulation have been done. The enclosure has been designed with high stress to minimize negative effect of the enclosure to the final sound quality.
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Prescher, Clemens [Verfasser], and Leonid [Akademischer Betreuer] Dubrovinsky. "Iron spin crossovers at high pressures and temperatures and their effects on materials relevant tot he Earth’s lower mantle and core / Clemens Prescher. Betreuer: Leonid Dubrovinsky." Bayreuth : Universität Bayreuth, 2014. http://d-nb.info/1059352397/34.
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Darrier, Benoît. "Cartographie fine de la recombinaison, analyse des séquences locales et étude du déséquilibre de liaison chez le blé tendre (Triticum aestivum)." Thesis, Clermont-Ferrand 2, 2016. http://www.theses.fr/2016CLF22764/document.
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Mieux connaitre les facteurs qui gouvernent l’apparition des évènements de recombinaison (crossing-overs ; CO) chez le blé tendre (Triticum aestivum L.) est primordial car ce processus est l’outil principal du sélectionneur pour permettre le brassage de la diversité génétique et l’introgression de régions d’intérêt dans des variétés agronomiques. L’utilisation de techniques de cytogénétique développées sur l’orge a permis de comparer la mise en place de la synapse lors de la méiose chez des lignées de blé tendre délétées de tout ou partie du bras long du chromosome 3B et qui avaient été préalablement montrées comme présentant un nombre de chiasmas réduit par rapport à la variété euploïde. Les analyses cytogénétiques couplées à des études bioinformatiques de la séquence montrent que le synapsis a lieu quasiment normalement chez les mutants et que la délétion de certains gènes connus comme impactant le déroulement de la méiose pourrait expliquer le phénotype observé. De plus, le développement de ressources génomiques (SNP, séquence) à destination des sélectionneurs a permis la réalisation de cartes génétiques haute densité des 21 chromosomes ancrées sur la séquence du génome. Tous les chromosomes montrent le même profil de recombinaison avec un accroissement dans les parties distales et une réduction drastique dans les parties centromériques et péri-centromériques. L’exploitation de plus de 250 CO localisés dans des fenêtres de moins de 25 kb sur le chromosome 3B utilisé comme modèle pour l’étude de l’impact de la séquence sur la recombinaison, montre que les profils de recombinaison ancestrale et actuelle sont conservés et que les CO ont lieu préférentiellement dans les parties promotrices des gènes exprimés en méiose ce qui suggère que la conformation chromatinienne influence la recombinaison. Finalement, ces données ont aussi été l’opportunité de détecter des motifs liés à la recombinaison qui présentent des similarités avec celui ciblé par la protéine PRDM9 qui conduit à la recombinaison chez l’humain. Cela suggère que les mécanismes de contrôle de la recombinaison sont conservés chez les eucaryotesBetter knowledge of the factors that drive recombination (crossovers; COs) in bread wheat (Triticum aestivum L.) is of main interest since this process is the main tool allowing breeders to admix the genetic diversity and to introgress regions of interest in agronomic varieties. We used cytogenetic techniques previously developed on barley to compare the establishment of synapsis during meiosis in deletion lines missing part or whole of the long arm of chromosome 3B of bread wheat and which were previously shown as having a reduced chiasmata number compared to euploid varieties. Cytogenetic analysis combined with bioinformatics studies showed that the synapsis occurs almost normally in mutants and that deletion of some genes known to impact meiosis behavior may explain the observed phenotype. In addition, development of genomic resources (SNPs, sequence) for wheat breeders allowed simultaneous elaboration of high density genetic maps for the 21 chromosomes anchored on genome sequence. All chromosomes present the same recombination pattern with an increase of recombination in the distal parts and reduction in centromeric/pericentromeric regions of the chromosomes. Analysis of more than 250 COs mapped in windows lower than 25kb located on chromosome 3B used as model to study the impact of sequence features on recombination showed that current and ancestral recombination patterns are conserved and that COs preferentially occur in the promoter part of gene expressed in meiosis suggesting that chromatin conformation impacts recombination. Finally, these data were the opportunity to detect recombination-associated motif which presents similarities with the motif targeted by the PRDM9 protein driving recombination in human. This suggests that the control of recombination mechanisms is conserved among eukaryotes
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Tejnorová, Petra. "Crossover." Doctoral thesis, Akademie múzických umění v Praze.Divadelní fakulta. Knihovna, 2016. http://www.nusl.cz/ntk/nusl-252296.
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This dissertation entitled CROSSOVER, between the layers of my own staging experience, on the boundaries of forms, genres and not only those, is divided into three parts. All sections are primarily based on my own theatre experience and are interlaced with numerous examples from performances and theatrically performative thinking of selected foreign ensembles. Given the subject matter, the first part primarily deals with Gob Squad and Shunt ensembles and the director Mike Leigh. In the second part, Rimini Protokoll and again Gob Squad are mentioned. The third part is dedicated mainly to the director Tim Etchells and his Forced Entertainment ensemble. My theatre experience is presented in the introductory part of this dissertation; starting with complex shows which represent a symbiosis of all components and are linked by their staging style and formerly discovered drama methods to authorial/devised projects to theatre which implements documentary and technological elements. In the first part of this dissertation, differences and subtle nuances between authorial and devised theatres are examined with focus on the specific devising creative method. The second part of the dissertation is focused on documentary and technologies. The third part consists of subtopics introduced in the previous chapters with respect to crossing boundaries between forms, genres and methodologies, which are more closely related to the phenomena of boundaries between theatre performances/events, fiction/reality, spectator/witness, actor/performer. The methods or the optics with which I work are based on the phenomenon of the mentioned areas and topics. The manner of addressing the issue can be likened to a phenomenological insight.
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Carvajal, Luis A. Rodriguez. "Multivariate crossover trials." Thesis, University of Warwick, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336783.
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Bauer, Wolfgang. "Understanding Spin Crossover: A Contribution." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-131697.
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Katkus, Kęstutis. "Hibridinis genetinis algoritmas komivojažieriaus uždaviniui." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2006. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20060606_203011-23232.
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In this work, the Traveling Salesman Problem (TSP) is discussed. The Hybrid Genetic Algorithm for solving the TSP is presented. The traveling salesman problem is formulated as follows: given matrix D=(dij)nxn of distances between n objects and the set P of permutations of the integers from 1 to n, find a permutation p=(p(1), p(2), ..., p(n)) P that minimizes. Many heuristic algorithms can be applied for the TSP. Recently, genetic algorithms (GAs) are among the advanced heuristic techniques for the combinatorial problems, like the TSP. genetic algorithms are based on the biological process of natural selection. The original concepts of GAs were developed in 1970s. Many simulations have demonstrated the efficiency of GAs on different optimization problems, among them, bin–packing, generalized assignment problem, graph partitioning, job–shop scheduling problem, set covering problem, vehicle routing. One of the main operators in GAs is the crossover (i.e. solution recombination). This operator plays a very important role by constructing competitive GAs. In this work, we investigate several crossover operators for the TSP, among them, CX (cycle crossover), PMX (partialy mapped crossover), POS (position based crossover), ER (edge recombination crossover), edge-NN (edge recombination crossover, nearest neighbour) and AP (alternating-positions crossover). Comparison of these crossover operators was performed. The results show high efficiency of the edge-NN, ER and PMX crossovers.
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Rezaei, Sadegh. "The mathematical analysis of crossover designs." Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phr4668.pdf.
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Xie, Changchun. "The analysis of binary crossover trials." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0026/MQ50090.pdf.
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Cerrito, Stefano. "Thermodynamics of the BCS-BEC crossover." kostenfrei, 2007. http://mediatum2.ub.tum.de/doc/623023/document.pdf.
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Bergen, Elvira [Verfasser]. "Multifunctional Spin Crossover Complexes / Elvira Bergen." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2021. http://d-nb.info/1225796032/34.
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Muggleton, David. "Crossover counterculture : postmodernism and spectacular style." Thesis, Lancaster University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364366.
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Hao, Chengcheng. "Explicit Influence Analysis in Crossover Models." Doctoral thesis, Stockholms universitet, Statistiska institutionen, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-107703.
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This dissertation develops influence diagnostics for crossover models. Mixed linear models and generalised mixed linear models are utilised to investigate continuous and count data from crossover studies, respectively. For both types of models, changes in the maximum likelihood estimates of parameters, particularly in the estimated treatment effect, due to minor perturbations of the observed data, are assessed. The novelty of this dissertation lies in the analytical derivation of influence diagnostics using decompositions of the perturbed mixed models. Consequently, the suggested influence diagnostics, referred to as the delta-beta and variance-ratio influences, provide new findings about how the constructed residuals affect the estimation in terms of different parameters of interest. The delta-beta and variance-ratio influence in three different crossover models are studied in Chapters 5-6, respectively. Chapter 5 analyses the influence of subjects in a two-period continuous crossover model. Possible problems with observation-level perturbations in crossover models are discussed. Chapter 6 extends the approach to higher-order crossover models. Furthermore, not only the individual delta-beta and variance-ratio influences of a subject are derived, but also the joint influences of two subjects from different sequences. Chapters 5-6 show that the delta-beta and variance-ratio influences of a particular parameter are decided by the special linear combination of the constructed residuals. In Chapter 7, explicit delta-beta influence on the estimated treatment effect in the two-period count crossover model is derived. The influence is related to the Pearson residuals of the subject. Graphical tools are developed to visualise information of influence concerning crossover models for both continuous and count data. Illustrative examples are provided in each chapter.
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Drewer, Holly B. "Reaction Time Crossover in Schizotypal Subjects." W&M ScholarWorks, 1990. https://scholarworks.wm.edu/etd/1539625639.
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Dsouza, Jeevan. "Region-based Crossover for Clustering Problems." NSUWorks, 2012. http://nsuworks.nova.edu/gscis_etd/139.
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Data clustering, which partitions data points into clusters, has many useful applications in economics, science and engineering. Data clustering algorithms can be partitional or hierarchical. The k-means algorithm is the most widely used partitional clustering algorithm because of its simplicity and efficiency. One problem with the k-means algorithm is that the quality of partitions produced is highly dependent on the initial selection of centers. This problem has been tackled using genetic algorithms (GA) where a set of centers is encoded into an individual of a population and solutions are generated using evolutionary operators such as crossover, mutation and selection. Of the many GA methods, the region-based genetic algorithm (RBGA) has proven to be an effective technique when the centroid was used as the representative object of a cluster (ROC) and the Euclidean distance was used as the distance metric.
The RBGA uses a region-based crossover operator that exchanges subsets of centers that belong to a region of space rather than exchanging random centers. The rationale is that subsets of centers that occupy a given region of space tend to serve as building blocks. Exchanging such centers preserves and propagates high-quality partial solutions.
This research aims at assessing the RBGA with a variety of ROCs and distance metrics. The RBGA was tested along with other GA methods, on four benchmark datasets using four distance metrics, varied number of centers, and centroids and medoids as ROCs. The results obtained showed the superior performance of the RBGA across all datasets and sets of parameters, indicating that region-based crossover may prove an effective strategy across a broad range of clustering problems.
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Klar, Vít. "Rekonstrukce železniční stanice Veselí nad Moravou." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2017. http://www.nusl.cz/ntk/nusl-265621.
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The subject of the Master's thesis is to design the redevelopment of the Veseli nad Moravou railway station. This renovation will include renewal of both, railway superstructure and substructure, including drainage system and new platform construcion proposal in order to meet current legislation regarding access of persons with reduced mibility and orientation skills. This thesis also deals with design of the track geometry compoments and geometry of set of switches at both heads of the railway station to fulfill the maximum train velocity demands at certain tracks. Last but not least, the rail track scheme of the station has been simplified.
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Petit, Morgane. "Etude des patrons de recombinaison, de leur déterminisme génétique et de leurs impacts en sélection génomique." Thesis, Toulouse, INPT, 2017. http://www.theses.fr/2017INPT0083/document.
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La recombinaison génétique est un processus biologique fondamental, ayant lieu au cours de la méiose et assurant la bonne ségrégation des chromosomes, ainsi que le maintien de la variabilité génétique grâce au brassage intrachromosomique. La recombinaison a été étudiée dans de nombreuses espèces, en particulier chez les Mammifères et les animaux d’élevage, comme les bovins, les porcs ou les ovins. Dans tous les cas, une variation du taux de recombinaison a été observée entre les individus et il a été démontré qu’elle était héritable et sous déterminisme génétique. Dans certaines espèces, des cartes génétiques ont également été construites, ce qui a permis de localiser les crossingovers et de détecter de très petites zones du génome où la recombinaison était importante : les points chauds. En race ovine Lacaune, de nombreuses données de génotypages sont disponibles, notamment grâce à l’existence de deux puces : une de moyenne densité avec 54 000 marqueurs et une de haute densité avec 600 000 marqueurs. Deux jeux de données étaient donc disponibles ; un jeu de données familial avec près de 6 000 individus apparentés et génotypés pour les 54 000 marqueurs et un jeu de données comportant 70 Lacaune non apparentés et génotypés pour les 600 000 marqueurs. Des cartes génétiques ont donc été créées pour ces deux jeux de données. Avec les animaux non apparentés, environ 50 000 points chauds ont été détectés. Le jeu de données familial a permis d’observer des motifs de distribution de la recombinaison communs aux autres Mammifères. Enfin, la combinaison des deux jeux de données a révélé la présence de signatures de sélection et a permis de créer une carte génétique de haute densité. De plus, une variation du taux de recombinaison a été observée entre les individus et a pu être liée à l’existence de 2 QTLs majeurs sur les chromosomes 6 et 7. Des gènes candidats plus ou moins bien connus ont pu être proposés, voire étudiés : RNF212 et HEI10. De plus, une comparaison avec une autre population ovine a permis de montrer que les cartes de recombinaison étaient quasiment identiques, mais que le taux de recombinaison individuel était soumis à un déterminisme génétique différent. Il a également été possible de proposer une application concrète pour l’utilisation des cartes génétiques en sélection génomique, grâce à la création de puces basse densité pouvant être utilisées pour l’imputation des reproducteurs et donc favoriser le génotypage et la sélection génomique à moindre coûtGenetic recombination is a fundamental biological process, which occurs during the meiosis. It allows the good segregation of the chromosomes and contributes to maintain the genetic diversity. Recombination was already studied in a lot of different species, especially in mammals and in farm animals, such as the pig, the cattle or the sheep. In each case, a variation of the recombination rate between the individuals was observed. This variation was heritable and under genetic determinism. In some species, genetic recombination maps were also created, which allowed to localize the crossovers and to detect really tiny genomic regions where the recombination is huge: the recombination hotspots. In the Lacaune breed sheep, a lot of genotyping data are available thanks to two existing arrays: a first with a medium density of markers (about 54,000 markers) and a second with a high density of markers (about 600,000 markers). Two datasets were thus available: a familial dataset with about 6,000 animals genotyped for the 54,000 markers and a dataset of 70 unrelated Lacaune genotyped for the 600,000 markers. Genetic recombination maps were created for these two datasets. With the 70 unrelated Lacaune, about 50,000 hotspots were detected. The familial dataset allowed to observe the mammals common recombination patterns. Finally, when the two datasets were combined, selection signatures were revealed and a high density recombination map were created. Furthermore, a variation of the recombination rate within the individuals was observed and was associated to 2 main QTLs on the chromosomes 6 and 7. Already known, or not, candidate genes were proposed and sometimes studied: especially RNF212 and HEI10. Finally, a comparison with another sheep breed revealed that the genetic recombination maps were really similar, but the individual recombination rate was under a different genetic determinism. A concrete application of the genetic recombination map in genomic selection was also proposed thanks to the creation of lowdensity SNPs sets, which could be used to impute the animals and thus to improve the genotyping and the genomic selection for lessercosts
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Bauer, Wolfgang [Verfasser]. "Understanding Spin Crossover: A Contribution / Wolfgang Bauer." München : Verlag Dr. Hut, 2011. http://d-nb.info/1014848571/34.
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Craig, Gavin. "Advanced Study of Switchable Spin Crossover Compounds." Doctoral thesis, Universitat de Barcelona, 2013. http://hdl.handle.net/10803/119688.
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Systems based on the organic ligand 3-bpp display a wide variety of magnetic behaviour, and have been studied in depth in the field of spin crossover (SCO). However, despite the apparent richness of SCO behaviour observed for 3-bpp systems, at the time of undertaking this thesis there were no published examples of the use of synthetic derivatives of 3-bpp in this field of research. This was remarked upon by Olguín and Brooker, who attributed this lack of 3-bpp analogues not to a lack of interest, but to “synthetic issues”. Therefore, the initial challenge set out was to develop a methodology that would allow the synthesis of functionalised 3-bpp ligands. Once obtained, these ligands would then be used for the generation of mononuclear Fe(II) compounds, with a view to study their macroscopic properties. Provided that this strategy was successful, and depending on the availability of structural data, an attempt would then be made to outline correlations between the observed magnetic properties and the relationship between the lattice entities, both spin-active and –inactive. The development of this work is detailed over the course of the ten Chapters. The first is an Introduction, primarily to the general field of SCO, the theory underlying its occurrence and its potential utility, but also describes the latest advances in the field before focussing on the family of 3-bpp systems.
Chapter 2 contains the synthetic procedures and experimental details for the physical characterisation of the compounds obtained. Chapter 3 then describes the development of the novel polypyrazolyl ligands that have been designed for the subsequent investigation of their coordination chemistry. The magneto-structural properties of a new [Fe(3-bpp)2]2+ salt are described, and the compound shown to display a gradual spin crossover.
Chapters 4 and 5 provide an in-depth study of a mononuclear compound, [Fe(H4L)2](ClO4)2•H2O•2(CH3)2CO (1), containing one of the polypyrazolyl ligands. A strongly cooperative spin transition is found to be intimately related to the structure and level of disorder within the lattice. This conclusion is supported by a description of the thermally trapped crystal structure. The photo-physical properties of 1 are then described, through the study of its meta-stable HS phase, which is distinct to the thermally trapped meta-stable HS phase. A Raman spectroscopy study of the system is then used to observe photo-switching within the hysteresis loop, and as a means of following the pressure-induced spin switch.
Chapters 6 and 7 use compound 1 as the basis of an investigation into the effect of varying the anions and solvents within the lattice on the magnetic properties and crystal packing. A case where the spin crossover becomes more cooperative on ageing the compound is described. Chapter 8 details the compounds obtained using another 3-bpp derivative, which results in the formation of distorted structures that do not display SCO.
In Chapter 9, the structural data provided by this thesis is collated with that found in the CSD for mononuclear Fe(II) systems with 3-bpp ligands. The data is then analysed from two points of view: a first, which involves previously defined parameters for the distortion of the cations, both in terms of their shape and in terms of the coordination sphere using Continuous Symmetry Measures. And a second, which uses Hirshfeld surface analysis to look at the bonding motifs and intermolecular interactions that are observed in the novel compounds obtained in this thesis. Chapter 10 offers conclusions based on the results obtained in this thesis.A pesar de la riqueza de los comportamientos de la transición de espín de los sistemas basados en el ligando 3-bpp, no se ha estudiado ningún derivado del ligando. Así pues, el reto inicial de esta tesis fue el de desarrollar ligandos derivados del 3-bpp. Estos se utilizarían en la síntesis de compuestos mononucleares de Fe(II), para estudiar sus propiedades macroscópicas. Este trabajo se detalla a lo largo de nueve capítulos. El capítulo 2 contiene los procedimientos experimentales para la síntesis y caracterización de los compuestos obtenidos. El capítulo 3 describe el desarrollo de los ligandos que fueron utilizados en la química de coordinación. Se detallan las propiedades magneto-estructurales de una nueva sal [Fe(3-bpp)2](ClO4)2 que presenta una transición de espín gradual. Los capítulos 4 y 5 estudian el compuesto [Fe(H4L)2](ClO4)2•H2O•2(CH3)2CO (1). Se observa que una transición de alta cooperatividad se asocia al desorden cristalográfico. Se procede a describir las propiedades foto-físicas del compuesto, mediante el estudio de su fase meta-estable de espín alto inducido por irradiación. Un estudio de espectroscopia de Raman se llevó a cabo, permitiendo así la observación de un proceso de “photo-switch” dentro del ciclo de histéresis. Los espectros obtenidos sirvieron para seguir la transición al aplicar una presión externa. Los capítulos 6 y 7 investigan el efecto de los aniones y disolventes sobre las propiedades magnéticas y el empaquetamiento estructural del compuesto 1. Se describe un caso dónde un proceso de envejecimiento del compuesto lleva a que se aumente su grado de cooperatividad. En el capítulo 8, se utiliza otro derivado del 3-bpp para preparar unos compuestos mononucleares de Fe(II). Debido a la deformación estructural presentada por estos compuestos, no se observa una transición de espín. En el capítulo 9, se recogen los datos estructurales obtenidos a lo largo de la tesis, y se combinan con los datos disponibles en el CSD para compuestos mononucleares de Fe(II). Se analizan los datos para medir la forma y distorsión de los cationes, y para estudiar los tipos de interacción intermolecular mediante los análisis de superficies de Hirshfeld. El capítulo 10 saca unas conclusiones basadas en los resultados descritos en los capítulos anteriores.
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Tovee, Clare Anne. "Metal complexes exhibiting spin crossover and fluorescence." Thesis, University of Leeds, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509875.
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Junginger, Andreas [Verfasser]. "Aufmerksamkeitsdefizit-/ Hyperaktivitässtörung: ein Crossover-Vergleich / Andreas Junginger." Ulm : Universität Ulm, 2019. http://d-nb.info/1197131973/34.
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Bews, R. "Digital crossover networks for active loudspeaker systems." Thesis, University of Essex, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383428.
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Filho, Márcio Antonio Ferreira Belo. "Lot sizing with setup carryover and crossover." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/55/55134/tde-29042016-142624/.
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Production planning problems are of paramount importance within supply chain planning, supporting decisions on the transformation of raw materials into finished products. Lot sizing in production planning refers to the tactical/operational decisions related to the size and timing of production orders to satisfy a demand. The objectives of lot-sizing problems are generally economical-related, such as saving costs or increasing profits, though other aspects may be taken into account such as quality of the customer service and reduction of inventory levels. Lot-sizing problems are very common in production activities and an efficient planning of such activities gives the company a clear advantage over concurrent organizations. To that end it is required the consideration of realistic features of the industrial environment and product characteristics. By means of mathematical modelling, such considerations are crucial, though their inclusion results in more complex formulations. Although lot-sizing problems are well-known and largely studied, there is a lack of research in some real-world aspects. This thesis addresses two main characteristics at the lot-sizing context: (a) setup crossover; and (b) perishable products. The former allows the setup state of production line to be carried over between consecutive periods, even if the line is not yet ready for processing production orders. The latter characteristic considers that some products have fixed shelf-life and may spoil within the planning horizon, which clearly affects the production planning. Furthermore, two types of perishable products are considered, according to the duration of their lifetime: medium-term and short-term shelf-lives. The latter case is tighter than the former, implying more constrained production plans, even requiring an integration with other supply chain processes such as distribution planning. Research on stronger mathematical formulations and solution approaches for lot-sizing problems provides valuable tools for production planners. This thesis focuses on the development of mixed-integer linear programming (MILP) formulations for the lot-sizing problems considering the aforementioned features. Novel modelling techniques are introduced, such as the proposal of a disaggregated setup variable and the consideration of lot-sizing instead of batching decisions in the joint production and distribution planning problem. These formulations are subjected to computational experiments in state-of-the-art MILP-solvers. However, the inherent complexity of these problems may require problemdriven solution approaches. In this thesis, heuristic, metaheuristic and matheuristic (hybrid exact and heuristic) procedures are proposed. A lagrangean heuristic addresses the capacitated lot-sizing problem with setup carryover and perishable products. A novel dynamic programming procedure is used to achieve the optimal solution of the uncapacitated single-item lot-sizing problem with setup carryover and perishable item. A heuristic, a fix-and-optimize procedure and an adaptive large neighbourhood search approach are proposed for the operational integrated production and distribution planning. Computational results on generated set of instances based on the literature show that the proposed methods yields competitive performances against other literature approaches.Problemas de planejamento da produção são de suma importância no planejamento da cadeia de suprimentos, dando suporte às decisões da transformação de matérias-primas em produtos acabados. O dimensionamento de lotes em planejamento de produção é definido pelas decisões tático-operacionais relacionadas com o tamanho das ordens de produção e quando fabricá-las para satisfazer a demanda. Os objetivos destes problemas são geralmente de cunho econômico, tais como a redução de custos ou o aumento de lucros, embora outros aspectos possam ser considerados, tais como a qualidade do serviço ao cliente e a redução dos níveis de estoque. Problemas de dimensionamento de lotes são muito comuns em atividades de produção e um planejamento eficaz de tais atividades, estabelece uma clara vantagem à empresa em relação à concorrência. Para este objetivo, é necessária a consideração de características realistas do ambiente industrial e do produto. Para a modelagem matemática do problema, estas considerações são cruciais, embora sua inclusão resulte em formulações mais complexas. Embora os problemas de dimensionamento de lotes sejam bem conhecidos e amplamente estudados, várias características reais importantes não foram estudadas. Esta tese aborda, no contexto de dimensionamento de lotes, duas características muito relevantes: (a) preservação da preparação total e parcial; e (b) produtos perecíveis. A primeira permite que o estado de preparação de uma linha de produção seja mantido entre dois períodos consecutivos, mesmo que a linha de produção ainda não esteja totalmente pronta para o processamento de ordens de produção. A ultima característica determina que alguns produtos tem prazo de validade fixo, menor ou igual do que o horizonte de planejamento, o que afeta o planejamento da produção. Além disso, de acordo com a duração de sua vida útil, foram considerados dois tipos de produtos perecíveis: produtos com tempo de vida de médio e curto prazo. O ultimo caso resulta em um problema mais apertado do que o anterior, o que implica em planos de produção mais restritos. Isto pode exigir uma integração com outros processos da cadeia de suprimentos, tais como o planejamento de distribuição dos produtos acabados. Pesquisas sobre formulações matemáticas mais fortes e abordagens de solução para problemas de dimensionamento de lotes fornecem ferramentas valiosas para os planejadores de produção. O foco da tese reside no desenvolvimento de formulações de programação linear inteiro-mistas (MILP) para os problemas de dimensionamento de lotes, considerando as características mencionadas anteriormente. Novas técnicas de modelagem foram introduzidas, como a proposta de variáveis de preparação desagregadas e a consideração de decisões de dimensionamento de lotes ao invés de decisões de agrupamento de ordens de produção no problema integrado de planejamento de produção e distribuição. Estas formulações foram submetidas a experimentos computacionais em MILP-solvers de ponta. No entanto, a complexidade inerente destes problemas pode exigir abordagens de solução orientadas ao problema. Nesta tese, abordagens heurísticas, metaheurísticas e matheurísticas (híbrido de métodos exatos e heurísticos) foram propostas para os problemas discutidos. Uma heurística lagrangeana aborda o problema de dimensionamento de lotes com restrições de capacidade, preservação da preparação total e produtos perecíveis. Um novo procedimento de programação dinâmica e utilizado para encontrar a solução ótima do problema de dimensionamento de lotes de um único produto perecível, sem restrições de capacidade e preservação da preparação total. Uma heurística, um procedimento x-and-optimize e uma abordagem por buscas adaptativas em grande vizinhanças são propostas para o problema integrado de planejamento de produção e distribuição. Resultados computacionais em conjuntos de instâncias geradas com base na literatura mostram que os métodos propostos obtiveram performances competitivas com relação a outras abordagens da literatura.
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Khademian, Hossein. "Caractérisation d’un point chaud de recombinaison méiotique chez Arabidopsis thaliana." Thesis, Paris 11, 2012. http://www.theses.fr/2012PA112051/document.
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La recombinaison méiotique initiée en prophase I de méiose génère soit des crossing-over (COs), qui sont des échanges réciproques entre segments chromosomiques, ou des conversions géniques non associées aux COs (NCOs). Les deux types d'événements se produisent dans de petites régions (moins de 10 kilobases) appelées points chauds, qui sont distribuées de manière non homogène le long des chromosomes. L'objectif de ma thèse était la caractérisation d'un point chaud de recombinaison méiotique (nommée 14a) chez Arabidopsis thaliana (i) dans différentes accessions (ii) dans le mutant msh4, un gène impliqué dans la formation des COs. Dans les deux hybrides ColxLer et ColxWs (i) 14a a un taux très élevé de COs 0,85% et 0,49%, respectivement (ii) Les COs sont regroupés dans deux petites régions de quelques kilobases, 14a1 et 14a2 avec une distribution de type gaussienne observée aux points chauds décrits dans d'autres espèces (iii) 14a1 est aussi un point chaud de NCO avec un taux aussi élevé que celui des COs (0,5%) dans ColxLer (iv) un biais de l'initiation de recombinaison a été trouvé dans 14a1 aussi bien pour les COs que les NCOs dans le fond génétique ColxLer.Une réduction de la fréquence de CO a été observée dans le mutant msh4 dans le fond génétique ColxLer à 14a1 et 14a2 (6,4% et 18,7% par rapport au sauvage). Cela confirme le rôle précédemment connu de la protéine MSH4 impliqué dans la formation de CO. La fréquence de NCO à 14a1 est similaire à celle observéedans le fond sauvage. Le rôle des H3K4 histones trimethyltransferase d’Arabidopsis dans la recombinaison méiotique (comme précédemment observé comme Set1 chez S. cerevisiae ou PRDM9 chez les mammifères) a également été étudiée. Aucun des dix gènes d’histones méthyltransférase étudié n'a montré de rôle dans la méiose. Cela pourrait être dû à (i) une forte redondance de la fonction entre les protéines (ii) une autre histone méthyltransférase en charge de l'étiquetage des points chauds de recombinaison méiotique (plus de 29 putatif histone méthyltransférase ont été identifiés dans le génome d'Arabidopsis!) (iii) contrairement à S. cerevisiae, les souris et l'homme, un autre mécanisme de contrôle épigénétique de la recombinaison méiotiqueMeiotic recombination initiated in prophase I of meiosis generates either crossovers (COs), which are reciprocal exchanges between chromosome segments, or gene conversion not associated to crossovers (NCOs). Both kinds of events occur in narrow regions (less than 10 kilobases) called hotspots, which are distributed non-homogenously along chromosomes. The aim of my PhD was the characterization of a hotspot of meiotic recombination (named 14a) in Arabidopsis thaliana (i) across different accessions (ii) in msh4 mutant, a gene involved in CO formation. In both ColxLer and ColxWs hybrids (i) 14a had a very high rate of COs 0.85% and 0.49%, respectively (ii) COs clustered in two small regions of a few kilobases, 14a1 and 14a2 with typical Gaussian curve distribution observed in other organisms (iii) 14a1 was also a hotspot of NCO with high rate (0.5%) in ColxLer (iv) a bias of recombination initiation at 14a1 CO and NCO hotspot was found in ColxLer. A reduction of CO frequency was observed in msh4 mutant in ColxLer background at 14a1 (6.4%) and 14a2 (18.7%) compared to wild type. This confirmed previously known role of MSH4 protein in CO formation. Frequency of NCO at 14a1 was similar to wild type. The role of putative Arabidopsis histone H3K4 trimethyltransferase in meiotic recombination as previously observed like Set1 in S.cerevisiae or PRDM9 in mammals (mice and human) was also studied. None of ten putative histone methyltransferase genes was involved in meiosis. This could be due to (i) a strong redundancy of function between gene products (ii) another histone methyltransferase in charge of labeling meiotic recombination hotspots (more than 29 putative histone methyltransferase have been identified in the Arabidopsis genome!) (iii) contrary to S. cerevisiae, mice and humans, another mechanism for epigenetic control of meiotic recombination
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Ibrahim, Wesam. "Linguistic approaches to crossover fiction : towards an integrated approach to the analysis of text worlds in children's crossover fantasy fiction." Thesis, Lancaster University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.684376.
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Wang, Quanyong. "Crossover in directional solidification and C60 island morphology." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32260.
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We simulate directional solidification using a phase field model solved with an adaptive finite element method. For small surface tension anisotropy directed at 45 degree relative to the pulling direction, we have observed three types of morphology: Dendritic, seaweed, and cellular. The seaweed pattern is characterized by alternating tip splitting and the branches have no orientation preference. A crossover from the seaweed to dendritic morphology was noticed when lowering the thermal gradient, consistent with previous experimental findings. We also investigate the influence of anisotropy on the morphology. We find that the thermal gradient, pulling velocity, and anisotropy can determine the crystal morphology. We show that the morphology of crystal structures can be unambiguously characterized through the local interface velocity distribution. As the thermal gradient is lowered, large-velocity shoulders begin to appear in both transverse and growth directions, becoming progressively broader as clearly defined dendrites emerge. We derive semi-empirically an estimate for the crossover from seaweed to dendrite as a function of thermal gradient and pulling velocity, which agrees with our simulation results. The ratio of thermal length to diffusion length has a sharp "transition" at the crossover region. In the second part of the thesis, a single variable dynamic model(Model B) is proposed to study C60 ultra-thin film growth on the alkali halide substrate. A triple-well free energy is proposed to explain the coexistence of three layers. The middle well is metastable representing a single layer. We successfully recover "branched" and "compact" patterns for both deposition aNous simulons la solidification directionnelle en utilisant un modèle de champ de phase résolu avec la méthode d'élément fini adaptative. Pour la petite anisotropie de tension superficielle dirigée à 45 degree relativement à la direction de traction, nous avons observé trois sortes de morphologies de solidification: dendritique, algue et cellulaire. Le schéma sous forme d'algues est caractérisé par le bout alternatif se dédoublant et dont les branches n'ont aucune préférence d'orientation. Une transition du schéma d'algues vers un morphologie dendritique a été noté lorsque le gradient thermique est abaissé. Ceci est compatible avec les résultats expérimentaux précédents. Nous étudions également l'influence de l'anisotropie sur la morphologie de cristal. Nous prouvons que le gradient thermique, la vitesse de traction, et l'anisotropie peuvent déterminer la morphologie de cristal. Nous prouvons que la morphologie des structures cristallines peut être clairement caractérisée par la distribution locale de la vitesse d'interface. Pendant que le gradient thermique est abaissé, les épaules de grand-vitesse commencent à apparaître dans des directions transversales et de croissance, devenant progressivement plus large en tant que dendrites bien définies émergent. Nous dérivons semi empiriquement une approximation pour la transition du schéma d'algues vers le schéma des dendrites en fonction du gradient thermique et la vitesse de traction, ce qui est conforme à nos résultats de simulation. Le rapport de la longueur thermique à la longueur de diffusion est caractérisée par une forte "transition" dans la région de la transition. Dans la
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Makubate, Boikanyo. "The place of crossover designs in infertility trials." Thesis, University of Glasgow, 2009. http://theses.gla.ac.uk/906/.
Full textAbstract:
Many people regard infertility as an unsuitable condition in which to run cross-over designs. Because if treatments are successful, then some women will become pregnant in the first period of treatment and if they become pregnant in the first period they are very unlikely to be treated in the second period. If data from only one period are available, then it will not be possible to perform the within-patient comparison. Hence, it could be argued, such designs are inherently unsuitable since they have a built-in tendency to produce missing data. To sum up this point of view, cross-over designs in infertility are likely to produce fewer data than one would wish and should be avoided. We see things differently, however. Suppose a parallel design is employed. If a couple is entered onto a parallel group design in infertility, they will have been allocated to one or the other treatment only. If the woman fails to achieve pregnancy, having been given that treatment, what could then be more natural than to offer the couple the chance of trying another? If another treatment is tried, then will it not be appropriate to record the outcome? Hence, in the worst case one will have all the data one would have from a parallel group trial but in practice one is likely to have more. How can more data be worse than less?Thus, to sum up our point of view, a cross-over trial is likely to produce more data than one would otherwise have had and should be encouraged. A debate along these lines has been running for some years now, with some promoting and occasionally running cross-over trials and others criticising them for doing so. In this thesis we use the logistic random effects model to illustrate that the message that the crossover design should be avoided is not the correct one. Rather, when using the crossover design one should be sure to analyze it correctly. The study has found that treatment estimates obtained by allowing women to get pregnant twice has lower standard errors than treatment estimates obtained by conducting the realistic infertility trials. In the scenario involving no period effects the two treatment estimates are not biased. In the scenario involving period effect, the standard errors of the treatment estimate obtained in the realistic data increases rapidly, while the standard errors of treatment estimate obtained by allowing women to get pregnant twice are not dissimilar from the standard errors obtained in the scenario involving no period effect. There is nothing wrong in conducting crossover designs in infertility provided appropriate statistical methods are employed. With the infertility crossover data set we can obtain not only conditional treatment estimates but also marginal estimates. Whereas in the parallel design we can only obtain marginal estimates. The study has found that if the treatment estimate say theta, can be obtained using parallel design data set, then surely, theta can be obtained using the crossover design data set, but not vice versa. Moreover the treatment estimate theta obtained using the crossover data set will be more consistent than the treatment estimate obtained using the parallel design. We recommend that crossover designs be used in infertility trials because it will surely benefit couples as couples will be have the opportunity to try both treatments.
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Shepherd, Helena Jane. "Spin crossover under extreme conditions : a structural approach." Thesis, Durham University, 2009. http://etheses.dur.ac.uk/228/.
Full textAbstract:
Structural studies of several iron complexes that show spin crossover (SCO) properties are presented and intrinsic links between the structure and properties have been established for these systems. X-ray diffraction experiments of samples under extremes of temperature and pressure and after irradiation with laser light have been performed to characterise the structure of these various compounds. The incomplete thermal SCO observed in the dinuclear species [
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Pawlus, Sebastian, Yoshi Hayashi, Kunal Kumar, and Alexei P. Sokolov. "Dynamic crossover in polymers, role of molecular weight." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-194211.
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