Relevant bibliographies by topics / Cryo-CLEM

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Cryo-CLEM'

Author: Grafiati
Published: 14 September 2024
Last updated: 31 July 2025

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Journal articles on the topic "Cryo-CLEM"

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Metskas, Lauren Ann, and John A. G. Briggs. "Fluorescence-Based Detection of Membrane Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy." Microscopy and Microanalysis 25, no. 4 (2019): 942–49. http://dx.doi.org/10.1017/s1431927619000606.

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AbstractCorrelated light and electron microscopy (CLEM) has become a popular technique for combining the protein-specific labeling of fluorescence with electron microscopy, both at room and cryogenic temperatures. Fluorescence applications at cryo-temperatures have typically been limited to localization of tagged protein oligomers due to known issues of extended triplet state duration, spectral shifts, and reduced photon capture through cryo-CLEM objectives. Here, we consider fluorophore characteristics and behaviors that could enable more extended applications. We describe how dialkylcarbocan
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Moser, Felipe, Vojtěch Pražák, Valerie Mordhorst, et al. "Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy." Proceedings of the National Academy of Sciences 116, no. 11 (2019): 4804–9. http://dx.doi.org/10.1073/pnas.1810690116.

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Correlative light and electron cryo-microscopy (cryo-CLEM) combines information from the specific labeling of fluorescence cryo-microscopy (cryo-FM) with the high resolution in environmental context of electron cryo-microscopy (cryo-EM). Exploiting super-resolution methods for cryo-FM is advantageous, as it enables the identification of rare events within the environmental background of cryo-EM at a sensitivity and resolution beyond that of conventional methods. However, due to the need for relatively high laser intensities, current super-resolution cryo-CLEM methods require cryo-protectants o
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Li, Shuoguo, Gang Ji, Xiaojun Huang, et al. "A New Solution of Non-integrated Correlative Light and Electron Microscopy Based on High-vacuum Optical Platform." Microscopy and Microanalysis 22, S3 (2016): 248–49. http://dx.doi.org/10.1017/s1431927616002099.

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Abstract Correlative light and electron microscopy (CLEM) offers a means of guiding the search for the unique or rare events by fluorescence microscopy (FM) and allows electron microscopy (EM) to zoom in on them for subsequent EM examination in three-dimensions (3D) and with nanometer-scale resolution. FM visualizes the localization of specific antigens by using fluorescent tags or proteins in a large field-of-view to study their cellular function, whereas EM provides the high level of resolution for complex structures. And cryo CLEM combines the advantages of maintaining structural preservati
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Thomas, Connon I., Nicolai T. Urban, Ye Sun, et al. "Cryo-Confocal Imaging for CLEM Mapping in Brain Tissues." Microscopy Today 29, no. 5 (2021): 34–39. http://dx.doi.org/10.1017/s1551929521001073.

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Abstract:In correlative light and electron microscopy (CLEM) workflows, identifying the same sub-cellular features in tissue by both light (LM) and electron microscopy (EM) remains a challenge. Furthermore, use of cryo-fixation for EM is desirable to capture rapid biological phenomena. Here, we describe a workflow that incorporates cryo-confocal laser scanning microscopy into the CLEM process, mapping cells in brain slices to re-image them with serial section scanning electron microscopy (ssSEM) array tomography. The addition of Airyscan detection increased the signal-to-noise ratio (SNR), all
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Hampton, Cheri M. "Practical Strategies for cryo-CLEM Experiments." Microscopy and Microanalysis 23, S1 (2017): 1400–1401. http://dx.doi.org/10.1017/s1431927617007668.

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Shahmoradian, Sarah. "Elucidating Tau Fibril Formation using Correlative Cryo-CLEM in situ." Structural Dynamics 12, no. 2_Supplement (2025): A351. https://doi.org/10.1063/4.0000657.

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Accurate modeling of tau protein aggregation in diverse cell systems, such as fluorescently tagged tau-expressing cell lines or 'biosensor' cells, is crucial for advancing tauopathy research. This encompasses understanding the early stages of tau fibril formation and localization within these cells, a vital but underexplored aspect in studying these neurodegenerative diseases. Our aim was to elucidate the structure and formation of tau fibrils in fluorescently tagged tau-expressing cell lines and iPSC- derived human neurons, assessing their resemblance to amyloid structures in tauopathies and
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Kamp, Arnold, Martijn van Nugteren, Hildo Vader, et al. "Automated Cryo-plunging Robot to Prepare Samples for Single Particle Analysis (SPA), Cryo-EM, Cryo-ET, Cryo-fluorescence and Cryo-CLEM." Microscopy and Microanalysis 26, S2 (2020): 2732–33. http://dx.doi.org/10.1017/s1431927620022606.

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Paraan, Reza, Victoria Hewitt, Yusuke Hirabayashi, Franck Polleux, Clint Potter, and Bridget Carragher. "Characterization of ER-mitochondria contact sites using cryo-CLEM." Microscopy and Microanalysis 27, S1 (2021): 1712–13. http://dx.doi.org/10.1017/s1431927621006255.

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Schwertner, Michael, and Duncan Stacey. "Cryo-Correlative Light and Electron Microscopy (Cryo-CLEM): Specimen Workflow Paths and Recent Instrument Developments." Microscopy and Microanalysis 21, S3 (2015): 1565–66. http://dx.doi.org/10.1017/s1431927615008600.

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Sexton, Danielle L., Steffen Burgold, Andreas Schertel, and Elitza I. Tocheva. "Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans." Current Research in Structural Biology 4 (2022): 1–9. http://dx.doi.org/10.1016/j.crstbi.2021.12.001.

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Dissertations / Theses on the topic "Cryo-CLEM"

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Patra, Gurudatt. "Structure of mitotic chromosome and the role of condensin protein in the structural organization of chromosomes." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAJ020.

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Au cours de la mitose, la chromatine interphasique subit une compaction massive en structures en forme de bâtonnets. Les condensines sont des complexes protéiques dont on sait qu'ils jouent un rôle majeur dans l'organisation des chromosomes mitotiques. Les eucaryotes possèdent deux complexes de condensines conservés, à savoir les condensines 1 et 2. Des études in vitro sur des modèles d'ADN nus montrent que les condensines ont une activité d'extrusion de boucles dans l'organisation des chromosomes. Cependant, il reste encore beaucoup à explorer en ce qui concerne l'étude de la fonction des con
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Lapios, Paul. "Ultrastructural and molecular analysis of cortico-striatal dopamine hub synapses." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0329.

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La dopamine est un neurotransmetteur qui module l'activité neuronale et régule des fonctions essentielles telles que la prédiction de la récompense, la motivation et le contrôle moteur. Dans le striatum, la dopamine agit généralement sur un large volume via des récepteurs métabotropes à action lente. Cependant, des études récentes ont montré que la dopamine peut également agir localement dans des espaces mesurant quelques micromètres. De plus, il a été démontré que la dopamine renforce la force synaptique lorsque sa libération coïncide avec l’action du glutamate. Ces découvertes suggèrent que
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Nocera, Giovanni Marco. "Microfluidic cryofixation for time-correlated live-imaging cryo-fluorescence microscopy and electron microscopy of Caenorhabditis elegans." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E4EA-8.

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Book chapters on the topic "Cryo-CLEM"

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Zanetti-Domingues, Laura C., Michael Hirsch, Lin Wang, et al. "Toward quantitative super-resolution methods for cryo-CLEM." In Methods in Cell Biology. Elsevier, 2024. http://dx.doi.org/10.1016/bs.mcb.2024.02.028.

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Conference papers on the topic "Cryo-CLEM"

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Smeets, Marit. "METEOR: an integrated top down cryo-CLEM imaging system." In Microscience Microscopy Congress 2021 incorporating EMAG 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.mmc2021.59.

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Schwertner, Michael. "Automated cryo-plunger for the preparation of vitrified cryo-samples for Single Particle Analysis (SPA), cryo-EM, cryo-ET and cryo-CLEM, based on a novel procedure replacing conventional blotting." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.1112.

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Schilling, Nicolas. "A cryo-preparation approach for immuno-fluorescence labelling of cell cultures and CLEM." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.867.

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You might also be interested in the extended bibliographies on the topic 'Cryo-CLEM' for particular source types:
Journal articles
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