Relevant bibliographies by topics / Cryo-SEM

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Cryo-SEM'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 February 2025

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Journal articles on the topic "Cryo-SEM"

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Makita, Takashi, Kazumi Hirabara, and Haruko Hirose. "Combination of cryo-SEM and WET-SEM." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 568–69. http://dx.doi.org/10.1017/s0424820100127360.

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WET-SEM is a version of commercially available SEM equipped with Robinson's type of wide-angle backscattered electron detector to observe wet samples under low vacuum(0.3-0.5 torr) and it has been used to variable biological samples with or without chemical fixation. Its versatility to observe hydrated specimens without any metalic coating is obviously advantageous to application of cryo-SEM to biological samples.Recent improvement of nitrogen gas cooled cold stage, and vacuum transfer device(Hexland, England) made the WET-SEM(ISI, Akashi, Japan) as a tool for quick survey of unfixed, hydrated, uncoated, and frozen fractured tissue blocks of animals. For examples, tissue from the rat liver or the mice kidney was quickly frozen in nitrogen slush for several minutes and then transfered to prechamber with a type of vacuum transfer system. Within the prechamber the surface of frozen sample is sublimated or fractured under vacuum and then the sample is ready to be seen on the cryo stage which is cooled by nitrogen gas.
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Sheehan, J. G., and L. E. Scriven. "Cryo-SEM of particulate suspensions." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 102–3. http://dx.doi.org/10.1017/s0424820100102596.

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Advances in particulate coatings for paper products and high-density recording media require an understanding of orientation, clustering, and cluster aggregation of submicron particles suspended in high volume fractions in liquid. Particles for paper coatings are typically platelets; for magnetic coatings, acicular, tabular, or compact metal oxide or composite grains. One goal is to visualize the dynamic microstructures behind the rheological properties of these suspensions.Fixation of liquid calls for cryo-techniques. Replication for TEM examination is difficult because clusters and cluster aggregates adhere to carbon support films in such a way as to interfere with imaging and even disrupt replicas. The best technique we have found is cryo-SEM with freeze-fracture and deep etching.A procedure similar to that for freeze-fracture replication for TEM was used. For each sample, a small drop was flattened between two copper sheets (Balzers BU 012 056-T and BU 012 054-T).
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Hermann, R., H. Hohenberg, H. H. Bauer, and M. Muller. "Progress in biological cryo-SEM." Proceedings, annual meeting, Electron Microscopy Society of America 52 (1994): 1010–11. http://dx.doi.org/10.1017/s0424820100172784.

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Field-emission SEM's of the in-lens type generate electron beam diameters of less than 1 nm at high accelerating voltages and of ca. 3-5 nm at 1 kV. These instruments, combined with a proper understanding of the involved signal generation and detection processes, represent one of the means to investigate biological surfaces with a structural information at the macromolecular level. In order to obtain this information, the biological structures have to be preserved as close as possible to the native state. This can be achieved with cryotechniques, e.g. cryoimmobilization, eventually followed by freeze-drying of the sample, metal coating at low specimen temperatures and lowtemperature observation in the SEM.Thin beam transparent samples can be visualized in this way with a structural resolution of ca. 3 nm. Fig. 1 shows a micrograph of differently polymerized human calcitonin: (a) a single fibril of ca. 8 nm diameter, exhibiting helical windings, and (b) a ribbon-like higher order structure, which is composed of individual fibrils.
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Nicholls, Daniel, Jack Wells, Alex W. Robinson, et al. "Compressive Cryo FIB-SEM Tomography." Microscopy and Microanalysis 29, Supplement_1 (2023): 526–27. http://dx.doi.org/10.1093/micmic/ozad067.247.

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Liberman, Lucy, Olga Kleinerman, Irina Davidovich, and Yeshayahu Talmon. "Micrograph contrast in low-voltage SEM and cryo-SEM." Ultramicroscopy 218 (November 2020): 113085. http://dx.doi.org/10.1016/j.ultramic.2020.113085.

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Choi, Yun-Joung, Kyung-Hwan Lee, A.-Reum Je, et al. "Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing." Korean Journal of Microscopy 42, no. 2 (2012): 111–14. http://dx.doi.org/10.9729/am.2012.42.2.111.

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Sheffield, E., E. G. Cutter, and W. A. Charlton. "Cryo SEM of reproduction in pteridophytes." Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences 86 (1985): 458–59. http://dx.doi.org/10.1017/s0269727000008630.

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Sutanto, E. "Cryo-SEM of liquid-bearing rock." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 104–5. http://dx.doi.org/10.1017/s0424820100102602.

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Scanning electron microscopy has been used to examine microstructure of dry soils, sedimentary rocks and other porous materials for three decades. There are many studies of sand grain surface texture, pore morphology, and clay swelling. However, pore geometry and surface topography are only part of the story of how two or more fluids flow through porous media, whether they be unconsolidated or consolidated. The other part is how the fluids distribute in the pore space. Fluid distribution in pore space is largely governed by relative wettability of pore walls. Wetting fluid tends to reside on walls as a thin film and to occupy small pores totally, whereas nonwetting fluid tends to occupy the center of larger pores. Which fluid is more strongly wetting depends on the local nature of the wall. Contact angles indicate wettability of planar, homogeneous solid surfaces, but roughness and compositional heterogeneity, which seem to be common in sedimentary rock, complicate matters.
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Liang, Jing, Feiyue Teng, Tseng-Ming Chou, and Matthew Libera. "Microgel Swelling Studied by Cryo-SEM." Microscopy and Microanalysis 23, S1 (2017): 1834–35. http://dx.doi.org/10.1017/s1431927617009837.

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Khawaja, Nazia N., David J. Goldfarb, Sachin Mittal, Irina Kazakevich, and Matthew Lamm. "Characterizing Dense Nanosuspensions using Cryo-SEM." Microscopy and Microanalysis 24, S1 (2018): 904–5. http://dx.doi.org/10.1017/s1431927618005019.

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Dissertations / Theses on the topic "Cryo-SEM"

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Deirieh, Amer (Amer Mohammad). "From clay slurries to mudrocks : a cryo-SEM investigation of the development of the porosity and microstructure." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/103704.

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Thesis: Ph. D. in Geotechnical and Geoenvironmental Engineering, Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, 2016.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 218-226).<br>This thesis investigates the microstructure of smectitic and illitic rich mudrocks that are resedimented in the laboratory from the Gulf of Mexico and Boston Blue clay natural sediments. The resedimentation technique consists of mixing clay sediments with saline water to form a slurry, which is then subjected to a wide range of effective stresses. Cryo-SEM and conventional SEM techniques are used to investigate the microstructure of the clay slurry and resedimented mudrocks. Cryo-SEM enables the imaging of samples in their in-situ conditions, while conventional SEM is used to image oven-dried samples. Two techniques are used to prepare the slurry samples for cryo-SEM imaging: high-pressure freezing and plunge freezing. The microstructure of high-pressure frozen clay slurries consists of individual clay particles and clay aggregates randomly distributed in water. These results demonstrate that one of the widely accepted models for clay slurries, the honeycomb structure, is an artifact of the plunge-freezing method and does not reflect the in-situ structure. In the field of geological sciences, this contrasts a fundamental view of fabric evolution that has inferred the presence of honeycomb structures that gradually evolve to an oriented fabric. Furthermore, we developed a method to obtain representative information about the heterogeneous pore space of mudrocks. This method combines information obtained from SEM images at different magnifications to represent the porosity of mudrocks. High magnification images provide high resolution and are used to sample small pores, while low magnification images provide low resolution and are used to sample large pores. The developed method provides a practical alternative to the current method that stitches together hundreds of images to obtain large representative mosaics. We show that SEM imaging detects only a fraction (~30%) of the total porosity of mudrocks, neglecting the significant amount of pores below the resolution of SEM. Furthermore, we show that oven drying, which is a prerequisite for most characterization methods, leads to a dramatic shrinkage (~50%) in the nanometer-sized clay pores, and does not influence the pore space visible in SEM images. Finally, we show that the application of stress on mudrocks leads to a simultaneous compression of inter-particle and intra-particle pores.<br>by Amer Deirieh.<br>Ph. D. in Geotechnical and Geoenvironmental Engineering
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Ikrényiová, Terézia. "Komplementární analýza prokaryotických buněk pomocí elektronové mikroskopie a Ramanovy spektroskopie." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-445147.

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This master thesis deals with conventional methods of bacterial cell analysis, polyhydroxyalkanoates, Raman spectroscopy and electron microscopy in the theoretical part. The production of polyhydroxybutyrate by selected thermophilic bacteria and their analysis by gas chromatography, cryogenic scanning electron microscopy and Raman spectroscopy is described in the experimental part. The chosen sample was analyzed by a transmission electron microscope. Comparing the results from previous mentioned methods it was found that the bacteria Schlegelella thermodepolymerans accumulated the highest amount of PHB. The lowest amount of PHB was obtained by bacteria Rubrobacter xylanophilus. The assumption that the PHB granules formed so-called needle-like plastic deformations during freeze-fracturing was affirmed by cryo-SEM photos analysis. Moreover, it was found that the bacterial cell characterization deduced from microscopic observation of samples corresponded to the description in the literature. TEM provided better resolution photos and in consequence the cells and PHB are more visible. The thesis is also focused on chemical fingerprint analysis of cells by Raman spectroscopy. Several biomolecules were identified by measured Raman spectra for the particular samples.
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Hrubanová, Kamila. "Scanning Electron Microscopy and its Applications for Sensitive Samples." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2019. http://www.nusl.cz/ntk/nusl-409082.

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Předložená dizertační práce s názvem “Rastrovací elektronová mikroskopie a její aplikace pro senzitivní vzorky” pojednává o problematice rastrovací elektronové mikroskopie v kontextu instrumentálního a metodologického vývoje vedoucího k inovativnímu řešení, které je dobře aplikovatelné zejména v mikrobiologickém výzkumu. Součástí práce je rozprava o historii a současném stavu elektronové mikroskopie (EM) jakožto vědecké zobrazovací a analytické techniky, tato část se nachází v úvodních kapitolách. Nepopiratelný přínos EM v biologických a lékařských oborech je dokazován mnoha citovanými vědeckými publikacemi. Předložená dizertační práce přináší novinky z oblasti přípravy preparátů a kryogenní rastrovací elektronové mikroskopie (cryo-SEM) vyvinuté na pracovišti Ústavu přístrojové techniky AV ČR, v.v.i. v Brně. Jedná se především o návrhy a výrobu speciálních držáků vzorků a vývoj nových metodik v oblasti přípravy mikrobiologických preparátů vedoucích k nalezení optimálních parametrů jednotlivých procesů. V experimentální části se nachází ověření metodologických postupů při studiu hydratovaných a na elektronový svazek senzitivních preparátů. Následné srovnání různých přístupů na definovaném biologickém systému z oblasti mikrobiologie přispívá k rozšíření interpretace doposud známých výsledků. Mezi zkoumanými mikrobiologickými kmeny byly biofilm-pozitivní bakterie Staphylococcus epidermidis a kvasinky jako Candida albicans a Candida parapsilosis, jež jsou považovány za klinicky významné, protože se podílejí na vzniku závažných infekcí zejména u imunokompromitovaných pacientů. Dále byl studován vliv růstu biofilmu bakterie Bacillus subtilis na biodeteriorizaci a biodegradaci poly--kaprolaktonových fólií. Vývoj v oblasti cryo-SEM byl aplikován ve výzkumu mikrobů s biotechnologickým potenciálem, jako jsou např. Cupriavidus necator a Sporobolomyces shibatanus.
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Duret, Bérénice. "Mise au point de dispersiοns aqueuses de particules d’huiles gélifiées et applications à la prοtectiοn de la peau". Electronic Thesis or Diss., Normandie, 2024. http://www.theses.fr/2024NORMLH39.

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Cette thèse vise à développer des formules cosmétiques éco-responsables à faible nombre d’ingrédients, en lien avec le contexte actuel du « Clean-label » dans ce secteur. Nous nous sommes intéressés à des dispersions de particules d’huile gélifiée, nommées « dispersions de gélosomes », encore inexplorées pour une utilisation cosmétique. Connues pour être stables et capables d’encapsuler des actifs hydrophobe, la question de leur texture et de leur application sur la surface de la peau reste aujourd’hui non renseignée. Elles sont préparées par émulsification à chaud d’un organogel, composé d’huile et de gélifiant lipophile (acide 12-hydroxystéarique), en présence d’un agent stabilisant (alcool polyvinylique hydrolysé à 80%). L’émulsion formée conduit, après refroidissement, à une dispersion de particules d’organogel. Nous avons d’abord démontré la possibilité de réaliser des dispersions de gélosomes avec des huiles cosmétiques et un conservateur. Des textures très variées ont été obtenues allant de liquides fluides à des gels fermes et cassants. L’analyse physico-chimique et l’observation microscopique de ces nouvelles formules ont permis d’identifier leurs microstructures : dans certaines conditions, des connexions se forment entre les gélosomes, et un hydrogel colloïdal est obtenu. L’étude des interactions à l’interface a permis de déterminer les facteurs et les mécanismes menant à des gélosomes individualisés ou connectés. Les dispersions de gélosomes, même les plus fluides, ont montré une grande stabilité. Enfin, de nouvelles dispersions de gélosomes ont pu être formulées à partir de stabilisants de nature et de modes de stabilisation variés. La méthodologie employée au cours de ce travail a permis d’établir un lien entre le stabilisant et les propriétés des dispersions. Des mécanismes différents ont pu être identifiés, induisant des microstructures et des propriétés applicatives intéressantes et variées. Pour la première fois, les propriétés de texture des dispersions, caractéristiques d’une application topique ont été collectées sur l’ensemble des systèmes par une approche combinée d’analyses rhéologiques in vitro et d’analyses sensorielles in vivo ; les perceptions ont été décrites et explicitées en fonction de l’influence de la nature de l’huile, du stabilisant et du type de microstructure<br>This thesis aims to develop eco-responsible cosmetic formulas with a low number of ingredients, in line with the current context of “Clean-label” in this sector. We focused on dispersions of gelled oil particles, called “gelosome dispersions”, which have not yet been explored for cosmetic use. Known to be stable and capable of encapsulating hydrophobic active ingredients, the question of their texture and their application onto the surface of the skin remains unanswered to date. They are prepared by hot emulsification of an organogel, composed of oil and a lipophilic gelator (12-hydroxystearic acid), in the presence of a stabilizing agent (80% hydrolyzed polyvinyl alcohol). Upon cooling, the emulsion leads to a dispersion of organogel particles. We first demonstrated the possibility of making gelosome dispersions with cosmetic oils and a preservative. A wide variety of textures was obtained, ranging from fluid liquids to firm and brittle gels. Physicochemical analysis and microscopic observation of these new formulas made it possible to identify their microstructures: under certain conditions, connections are formed between the gelosomes, and a colloidal hydrogel is obtained. The factors and mechanisms leading to individualized or connected gelosomes were determined by the study of interactions at the interface. Gelosome dispersions, even the most fluid, showed great stability. Finally, new dispersions of gelosomes were formulated using stabilizers of various types and stabilization modes. The methodology used during this work enabled the establishment of a link between the stabilizer and the properties of the dispersions. Different mechanisms could be identified, inducing interesting and varied microstructures and application properties. For the first time, the texture properties of the dispersions, characteristic of a topical application, were collected across all systems using a combined approach of in vitro rheological analyzes and in vivo sensory analyses; the perceptions were described and explained according to the influence of the nature of the oil, the stabilizer and the type of microstructure
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Sahlin-Platt, Annika. "Bone tissue regeneration indento-alveolar surgery : clinical and experimental studies on biomaterials and bone graft substitutes." Doctoral thesis, Umeå universitet, Institutionen för odontologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-47418.

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Pathological processes in the alveolar and facial bones can lead to bone loss that may not heal with complete regeneration. Biomaterials can be used to facilitate the healing process and/or as a bone substitute, but the mechanisms are not fully understood. Persistent leakage of bacteria/bacterial toxins, after root canal treatment, may lead to a residual bone defect. The healing is dependent on a placed dental biomaterial providing a tight seal. The composition of the filling material may also influence the healing process. The general aim of this study is to investigate surface properties and biological interactions of biomaterials used in dento-alveolar surgery. A dental biomaterial, a bonded compomer (DAP) containing a corroding glass filler, was used as a root end filling material, promoting a new operation technique. The healing (assessed according to Molven´s x-ray criteria) demonstrates a significant improvement in healing results for the compomer group, compared to a commonly used technique. The surface properties and biological interactions of DAP were analyzed. ICP-OES of DAP cell culture medium extract demonstrated a significant release of Sr, Si and F from the dental biomaterial. Human periodontal ligament (PDL) cells grew on and around DAP specimens without any sign of toxic reactions. DAP extract stimulated proliferation of PDL cells, but caused an inhibition of osteoblastic gene expression in mouse bone marrow cells. The surface properties of the glass containing compomer may contribute to improved healing of the periapical lesions. A bovine inorganic bone graft substitute (BO) is commonly used as a treatment option in dento-alveolar surgery with new bone formation in immediate close contact with BO material. ICP-OES dissolution analysis of cell culture media, after incubation with BO particles, demonstrated a dosedependent release of Si and a decrease of Ca and P. An uptake of Ca from the medium to the BO particle was demonstrated with calcium-45 labeling. The Si dissolution varied between different batches, possibly reflecting a variation in food intake in the animals. Stimulated osteogenic response was seen in close contact to the BO particles in cell cultures. Furthermore, it was clearly demonstrated that the study design is a critical factor for correctly understanding biomaterials’ biological interactions. The surface properties of three bone graft substitutes reported to have good results in dento-alveolar surgery were investigated, in order to establish whether or not dissolution-precipitation reactions could contribute to the bone healing. Dissolution-precipitation extracts of BO, bioactive glass 45S5 (BG) and a marine algae hydroxyl apatite (AP) in cell culture media were analyzed. Dissolution of Si at significant levels was detected for BO and 45S5 over time. Significant uptake levels of Ca and P from the culture were seen for both 45S5, BO and AP but at different times. Surface analysis of the biomaterials with SEM/EDAX, before and after immersion in cell culture media, revealed a smoothing of the surface morphology for 45S5 over time. No obvious alterations for BO and AP were detected. Ca/P ratio decreased significantly for 45S5, but no major changes were detected by XPS for BO or AP. XPS further demonstrated a surface charge for BO, changing from negatively to positively charged when exposed to serum. 45S5 and AP had positive surface charges, both in the absence and the presence of serum. These demonstrated surface changes in biomaterials could contribute to adherence of cells and subsequently affect bone healing. Conclusion: Biomaterials used in dento-alveolar surgery interact with biological surroundings through surface and dissolution-precipitation reactions which may have implications for bone healing.
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Skoupý, Radim. "Quantitative Imaging in Scanning Electron Microscope." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-432610.

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Tato práce se zabývá možnostmi kvantitativního zobrazování ve skenovacím (transmisním) elektronovém mikroskopu (S|T|EM) společně s jejich korelativní aplikací. Práce začíná popisem metody kvantitativního STEM (qSTEM), kde lze stanovenou lokální tloušťku vzorku dát do spojitosti s ozářenou dávkou, a vytvořit tak studii úbytku hmoty. Tato metoda byla použita při studiu ultratenkých řezů zalévací epoxidové pryskyřice za různých podmínek (stáří, teplota, kontrastování, čištění pomocí plazmy, pokrytí uhlíkem, proud ve svazku). V rámci této části jsou diskutovány a demonstrovány možnosti kalibračního procesu detektoru, nezbytné pozadí Monte Carlo simulací elektronového rozptylu a dosažitelná přesnost metody. Metoda je pak rozšířena pro použití detektoru zpětně odražených elektronů (BSE), kde byla postulována, vyvinuta a testována nová kalibrační technika založená na odrazu primárního svazku na elektronovém zrcadle. Testovací vzorky byly různě tenké vrstvy v tloušťkách mezi 1 až 25 nm. Použití detektoru BSE přináší možnost měřit tloušťku nejen elektronově průhledných vzorků jako v případě qSTEM, ale také tenkých vrstev na substrátech - qBSE. Obě výše uvedené metody (qSTEM a qBSE) jsou založeny na intenzitě zaznamenaného obrazu, a to přináší komplikaci, protože vyžadují správnou kalibraci detektoru, kde jen malý posun úrovně základního signálu způsobí významnou změnu výsledků. Tato nedostatečnost byla překonána v případě qSTEM použitím nejpravděpodobnějšího úhlu rozptylu (zachyceného pixelovaným STEM detektorem), namísto integrální intenzity obrazu zachycené prstencovým segmentem detektoru STEM. Výhodou této metody je její použitelnost i na data, která nebyla předem zamýšlena pro využití qSTEM, protože pro aplikaci metody nejsou potřeba žádné zvláštní předchozí kroky. Nevýhodou je omezený rozsah detekovatelných tlouštěk vzorku způsobený absencí píku v závislosti signálu na úhlu rozptylu. Obecně platí, že oblast s malou tloušťkou je neměřitelná stejně tak jako tloušťka příliš silná (použitelný rozsah je pro latex 185 - 1 000 nm; rozsah je daný geometrií detekce a velikostí pixelů). Navíc jsou v práci prezentovány korelativní aplikace konvenčních a komerčně dostupných kvantitativních technik katodoluminiscence (CL) a rentgenové energiově disperzní spektroskopie (EDX) spolu s vysokorozlišovacími obrazy vytvořenými pomocí sekundárních a prošlých elektronů.
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Book chapters on the topic "Cryo-SEM"

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Morrison, Robert. "SEM Cryo-Stages and Preparation Chambers." In Biological Field Emission Scanning Electron Microscopy. John Wiley & Sons, Ltd, 2019. http://dx.doi.org/10.1002/9781118663233.ch8.

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von Both, Holger, Rainer Oberacker, and Michael J. Hoffmann. "Structural Analysis of Ceramic Suspensions by CRYO-SEM Investigations." In Metal Matrix Composites and Metallic Foams. Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527606203.ch8.

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Resch, Guenter P. "Cryo-SEM Specimen Preparation Workflows from the Leica Microsystems Design Perspective." In Biological Field Emission Scanning Electron Microscopy. John Wiley & Sons, Ltd, 2019. http://dx.doi.org/10.1002/9781118663233.ch9.

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Gorb, Elena V., and Stanislav N. Gorb. "Functional Surfaces in the Pitcher of the Carnivorous Plant Nepenthes alata: A Cryo-Sem Approach." In Functional Surfaces in Biology. Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-1-4020-6695-5_9.

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Vashishtha, Ankur. "ADVANCED MICROSCOPY TECHNIQUES IN RESEARCH AND MEDICAL DIAGNOSTICS." In MEDICAL DIAGNOSTICS: LABORATORY TO RADIOLOGICAL IMAGING. KAAV PUBLICATIONS, 2024. http://dx.doi.org/10.52458/9788196919528.2024.eb.ch-01.

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Advanced microscopy techniques have emerged as indispensable tools in scientific research, offering unprecedented capabilities to explore the intricate world of the nanoscale. This abstract provides a concise overview of the diverse array of cutting-edge microscopy methodologies that have redefined our ability to observe and analyze structures at resolutions once deemed unattainable. Super-resolution microscopy, including techniques such as Structured Illumination Microscopy (SIM) and Stochastic Optical Reconstruction Microscopy (STORM), has transcended traditional diffraction limits, revealing details at the molecular level in biological and material systems. Electron microscopy, exemplified by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and Cryo-Electron Microscopy (Cryo-EM), has revolutionized imaging by providing high-resolution insights into the structural nuances of diverse specimens. Scanning probe microscopy, with key players like Atomic Force Microscopy (AFM) and Scanning Tunneling Microscopy (STM), enables researchers to manipulate and characterize materials at the atomic and molecular scales, fostering breakthroughs in nanotechnology and materials science. The chapter also explores the integration of advanced microscopy with complementary techniques, such as fluorescence microscopy for dynamic live-cell imaging and correlative microscopy for a holistic understanding of complex systems. Throughout this exploration, the abstract emphasizes the transformative impact of advanced microscopy on interdisciplinary research, spanning fields from biology to materials science. The evolving landscape of microscopy technologies continues to shape our understanding of the nanoworld, promising new avenues for scientific discovery and technological innovation.
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Chang, Irene Y., Mohammad Rahman, Adam Harned, Orna Cohen-Fix, and Kedar Narayan. "Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm." In Methods in Cell Biology. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mcb.2020.09.009.

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Conference papers on the topic "Cryo-SEM"

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Hye Hyun, Ji, Yanchao Dai, In Chang Choi, and Christopher H. Kang. "The Impact of TEM Analysis Temperature on Photoresist Profiles Using Cryo-FIB and Cryo-TEM." In ISTFA 2024. ASM International, 2024. http://dx.doi.org/10.31399/asm.cp.istfa2024p0221.

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Abstract Photoresist (PR) profiles tend to have deformation and shrinkage with typical transmission electron microscopy (TEM) analysis method using a focused ion beam scanning electron microscope (FIB-SEM) and TEM. The elevated temperatures during sample preparation and TEM analysis are believed to contribute to these issues. This study evaluates the effectiveness of cryogenic workflow in mitigating PR profile shrinkage by employing cryo-focused ion beam (Cryo-FIB) and cryo-transmission electron microscopy (Cryo-TEM). Comparative experiments were conducted at room temperature and cryogenic conditions, demonstrating that full cryogenic workflow reduces the shrinkage of PR, bottom anti-reflective coating (BARC), and line critical dimension (CD). Our findings indicate that both the sample preparation and analysis temperatures influence PR profiles. This study highlights how the full cryogenic workflow significantly minimizes shrinkage, providing more accurate PR profile measurements.
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2

"Compressive Cryo FIB-SEM Tomography." In Microscience Microscopy Congress 2023 incorporating EMAG 2023. Royal Microscopical Society, 2023. http://dx.doi.org/10.22443/rms.mmc2023.161.

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3

El Howayek, Alain, Antonio Bobet, Cliff T. Johnston, Marika Santagata, and Joseph V. Sinfield. "Microstructure of Sand-Laponite-Water Systems Using Cryo-SEM." In Geo-Congress 2014. American Society of Civil Engineers, 2014. http://dx.doi.org/10.1061/9780784413272.067.

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4

Gmira, A., D. K. Cha, S. Al-Enezi, A. Yousef, and M. Al-Otaibi. "Cryo-BIB-SEM Imaging of Mixed Wettability in Carbonate Rocks." In 80th EAGE Conference and Exhibition 2018. EAGE Publications BV, 2018. http://dx.doi.org/10.3997/2214-4609.201800780.

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5

Watts, Julie. "Complementary cryo SEM and hybrid 3D ToF OrbiTrap SIMS for hydrogel characterisation." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.470.

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"Cryo-SEM to characterize the Microstructure of Starch during in vitro digestion." In Microscience Microscopy Congress 2023 incorporating EMAG 2023. Royal Microscopical Society, 2023. http://dx.doi.org/10.22443/rms.mmc2023.160.

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7

Bhavar, Valmik, Shreyans Khot, Prakash Kattire, Mohan Mehta, and R. K. P. Singh. "Effect of Deep Cryogenic Treatment on AISI H13 Tool Steel." In HT 2015. ASM International, 2015. http://dx.doi.org/10.31399/asm.cp.ht2015p0383.

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Abstract In the recent years, there has been a remarkable increase in the use of deep cryogenic treatment (DCT) for enhancing performance of tool steels. It is a supplementary treatment where components are treated below subzero temperatures for several cryo-soaking hours. This paper focuses on to study the effect of deep cryogenic treatment and cryo-soaking time on microstructural and mechanical properties of AISI H-13 tool steel. Deep cryogenic treatment at different cryo-soaking time (16-48 hours) were applied and tool steel performance was analyzed by using mechanical, fatigue and wear testings. The microstructural evolutions during DCT were evaluated by using scanning electron microscope (SEM). It was observed that microstructural modifications like increase in carbide density, fine and uniform martensitic structure during DCT had significantly improved properties which were influenced by cryo-soaking time.
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ROBIN, M., R. Combes, and E. Rosenberg. "Cryo-SEM and ESEM: New techniques to Investigate Phase Interactions within Reservoir Rocks." In SPE Annual Technical Conference and Exhibition. Society of Petroleum Engineers, 1999. http://dx.doi.org/10.2118/56829-ms.

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9

Tabe, Yutaka, Masataka Saito, Ryosuke Ichikawa, and Takemi Chikahisa. "Microscopic Observation of Ice Distribution in PEM Fuel Cell at Cold Start." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44517.

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In Polymer electrolyte membrane fuel cells (PEFCs), freezing of produced water induces the extreme deterioration of cell performance below zero. This phenomenon is a serious problem in cold regions and is needed to be solved to achieve the practical use of PEFCs. In this study, we investigated ice distribution at the cold start in a PEFC using an optical microscope and a CRYO-SEM to clarify the freezing mechanism. The observation results showed that the cold start at −10°C makes ice at the interface between the cathode catalyst layer (CL) and the micro porous layer of gas diffusion layer. Little ice was, however, observed in the cold start at −20°C, which indicated the ice formation inside the CL. The CRYO-SEM observation was conducted at −20°C to investigate the ice formation inside the CL, and this identified the effects of the current density and the cathode gas species on the ice distribution.
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Aoyama, Yusuke, Kengo Suzuki, Yutaka Tabe, and Takemi Chikahisa. "Analysis of Water Transport in the Vicinity of Micro-Porous Layer in PEFC With Freezing Method." In ASME 2013 11th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/icnmm2013-73124.

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This paper examines the role of micro porous layers (MPLs) in Polymer Electrode Fuel Cells (PEFCs) by observing the cross-sectional distribution of condensed water inside a cathode side MPL In addition, the forms of water condensation in the vicinity of a MPL are also compared between two places, under flow channels and under lands, by observing both inside the MPL and an interface between the MPL and a catalyst layer (CL). The freezing method and a cryo-scanning electronic microscope (cryo-SEM) are used for the observation. The result under the non-flooded condition shows that condensed water does not accumulate inside the MPL. This result indicates that the water produced by PEFC power generation passes through the MPL as vapor state under non-flooded conditions.
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