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1

Kück, Ulrich, and Stefanie Pöggeler. "Cryptic sex in fungi." Fungal Biology Reviews 23, no. 3 (August 2009): 86–90. http://dx.doi.org/10.1016/j.fbr.2009.10.004.

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2

Lopes, Anabela, Benedetto T. Linaldeddu, Alan J. L. Phillips, and Artur Alves. "Mating type gene analyses in the genus Diplodia: From cryptic sex to cryptic species." Fungal Biology 122, no. 7 (July 2018): 629–38. http://dx.doi.org/10.1016/j.funbio.2018.03.012.

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3

Grimsley, N., B. Pequin, C. Bachy, H. Moreau, and G. Piganeau. "Cryptic Sex in the Smallest Eukaryotic Marine Green Alga." Molecular Biology and Evolution 27, no. 1 (September 4, 2009): 47–54. http://dx.doi.org/10.1093/molbev/msp203.

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4

Kupriyanova, L. A., and L. D. Safronova. "Results and perspectives of cyto- and genetic studying of “cryptic” group of the Lacertidae." Proceedings of the Zoological Institute RAS 324, no. 1 (March 24, 2020): 100–107. http://dx.doi.org/10.31610/trudyzin/2020.324.1.100.

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Results of chromosomal and molecular studies of the lizard Zootoca vivipara (Lichtenstein, 1823) (Lacertidae) from many geographically separate populations of Europe and Asia have been generalized. The questions of ka­ryotype differences within the species, of diversity of its Zw and multiple Z1Z2W sex chromosome, their reorganizations and evolutionary consequences have been briefly considered. Stability of forming karyotypes is as an integrating factor which allow to identify the specimens and unite them into the groups possessing the distinct distribution areas. There are a correlation between chromosomal, mt DNA and nuclear DNA data. Finally all data obtained allow to draw a conclusion that Z. vivipara represents a cryptic group of cryptic taxa. Besides new data about the behavior of multiple sex chromosomes (SC, synaptonemal complexes) in early meiosis and molecular-cytogenetic data on transposable elements (TE) in the genome of Z. vivipara, their localization in the definite regions of chromosomes may suggest that they play a role in active speciation process by formation of cryptic taxa.
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5

Alam, Shayer Mahmood Ibney, Tulyawat Prasongmaneerut, Dianne Gleeson, Arthur Georges, Stephen D. Sarre, Kornsorn Srikulnath, and Tariq Ezaz. "Sex-Determination Mechanisms among Populations within Cryptic Species Complex of Calotes (Squamata: Agamidae: Draconinae)." DNA 1, no. 2 (October 6, 2021): 49–67. http://dx.doi.org/10.3390/dna1020006.

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Sex-determination mechanisms and sex chromosomes are known to vary among reptile species and, in a few celebrated examples, within populations of the same species. The oriental garden lizard, Calotes versicolor, is one of the most intriguing species in this regard, exhibiting evidence of multiple sex-determination modes within a single species. One possible explanation for this unusual distribution is that in C. versicolor, different modes of sex determination are confined to a particular population or a species within a cryptic species complex. Here, we report on a population genetic analysis using SNP data from a methylation-sensitive DArT sequencing analysis and mitochondrial DNA data obtained from samples collected from six locations: three from Bangladesh and three from Thailand. Our aim was to determine whether C. versicolor is best described as a single species with multiple lineages or as multiple species, as well as if its sex-determination mechanisms vary within or between species. We present evidence that the latter possibility is the case and that C. versicolor comprises a complex of cryptic species. We also identify sex-linked markers within these species and use them to identify modes of sex determination. Overall, our results suggest that different sex-determination modes have evolved among closely related species and within populations of Agamid lizards.
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6

El-Ballal, A. S. I. "CRYPTIC POLYMORPHISM OF SEX EXPRESSION IN CUMIN (CUMINUM CYMINUM, L.)." Acta Horticulturae, no. 208 (August 1987): 197–208. http://dx.doi.org/10.17660/actahortic.1987.208.21.

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7

Kratochvíl, Lukáš, Jasna Vukić, Jan Červenka, Lukáš Kubička, Martina Johnson Pokorná, Dominika Kukačková, Michail Rovatsos, and Lubomír Piálek. "Mixed‐sex offspring produced via cryptic parthenogenesis in a lizard." Molecular Ecology 29, no. 21 (September 30, 2020): 4118–27. http://dx.doi.org/10.1111/mec.15617.

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8

König, Christian, Johannes L. M. Steidle, and Till Tolasch. "Decrypting Cryptic Click Beetle Species by Analysis of Sex Pheromones." Journal of Chemical Ecology 41, no. 8 (August 2015): 740–45. http://dx.doi.org/10.1007/s10886-015-0606-6.

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9

Whiteley, Sarah L., Arthur Georges, Vera Weisbecker, Lisa E. Schwanz, and Clare E. Holleley. "Ovotestes suggest cryptic genetic influence in a reptile model for temperature-dependent sex determination." Proceedings of the Royal Society B: Biological Sciences 288, no. 1943 (January 20, 2021): 20202819. http://dx.doi.org/10.1098/rspb.2020.2819.

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Sex determination and differentiation in reptiles is complex. Temperature-dependent sex determination (TSD), genetic sex determination (GSD) and the interaction of both environmental and genetic cues (sex reversal) can drive the development of sexual phenotypes. The jacky dragon ( Amphibolurus muricatus ) is an attractive model species for the study of gene–environment interactions because it displays a form of Type II TSD, where female-biased sex ratios are observed at extreme incubation temperatures and approximately 50 : 50 sex ratios occur at intermediate temperatures. This response to temperature has been proposed to occur due to underlying sex determining loci, the influence of which is overridden at extreme temperatures. Thus, sex reversal at extreme temperatures is predicted to produce the female-biased sex ratios observed in A. muricatus . The occurrence of ovotestes during development is a cellular marker of temperature sex reversal in a closely related species Pogona vitticeps . Here, we present the first developmental data for A. muricatus , and show that ovotestes occur at frequencies consistent with a mode of sex determination that is intermediate between GSD and TSD. This is the first evidence suggestive of underlying unidentified sex determining loci in a species that has long been used as a model for TSD.
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10

Gemeno, C., N. Laserna, M. Riba, J. Valls, C. Castañé, and O. Alomar. "Cuticular hydrocarbons discriminate cryptic Macrolophus species (Hemiptera: Miridae)." Bulletin of Entomological Research 102, no. 6 (April 17, 2012): 624–31. http://dx.doi.org/10.1017/s0007485312000193.

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AbstractMacrolophus pygmaeus is commercially employed in the biological control of greenhouse and field vegetable pests. It is morphologically undistinguishable from the cryptic species M. melanotoma, and this interferes with the evaluation of the biological control activity of M. pygmaeus. We analysed the potential of cuticular hydrocarbon composition as a method to discriminate the two Macrolophus species. A third species, M. costalis, which is different from the other two species by having a dark spot at the tip of the scutellum, served as a control. Sex, diet and species, all had significant effects in the cuticular hydrocarbon profiles, but the variability associated to sex or diet was smaller than among species. Discriminant quadratic analysis of cuticular hydrocarbons confirmed the results of previous molecular genetic studies and showed, using cross-validation methods, that M. pygmaeus can be discriminated from M. costalis and M. melanotoma with prediction errors of 6.75% and 0%, respectively. Therefore, cuticular hydrocarbons can be used to separate M. pygmaeus from M. melanotoma reliably.
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11

Butler, Geraldine. "Fungal Sex and Pathogenesis." Clinical Microbiology Reviews 23, no. 1 (January 2010): 140–59. http://dx.doi.org/10.1128/cmr.00053-09.

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SUMMARYHuman fungal pathogens are associated with diseases ranging from dandruff and skin colonization to invasive bloodstream infections. The major human pathogens belong to theCandida,Aspergillus, andCryptococcusclades, and infections have high and increasing morbidity and mortality. Many human fungal pathogens were originally assumed to be asexual. However, recent advances in genome sequencing, which revealed that many species have retained the genes required for the sexual machinery, have dramatically influenced our understanding of the biology of these organisms. Predictions of a rare or cryptic sexual cycle have been supported experimentally for some species. Here, I examine the evidence that human pathogens reproduce sexually. The evolution of the mating-type locus in ascomycetes (includingCandidaandAspergillusspecies) and basidiomycetes (MalasseziaandCryptococcus) is discussed. I provide an overview of how sex is suppressed in different species and discuss the potential associations with pathogenesis.
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12

Kurpyianova, Larissa, and Larissa Safronova. "A Brief Review of Meiotic Chromosomes in Early Spermatogenesis and Oogenesis and Mitotic Chromosomes in the Viviparous Lizard Zootoca vivipara (Squamata: Lacertidae) with Multiple Sex Chromosomes." Animals 13, no. 1 (December 20, 2022): 19. http://dx.doi.org/10.3390/ani13010019.

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This brief review is focused on the viviparous lizard Zootoca vivipara (Lichtenstein, 1823), of the family Lacertidae, which possesses female heterogamety and multiple sex chromosomes (male 2n = 36, Z1Z1Z2Z2/Z1Z2W, female 2n = 35, with variable W sex chromosome). Multiple sex chromosomes and their changes may influence meiosis and the female meiotic drive, and they may play a role in reproductive isolation. In two cryptic taxa of Z. vivipara with different W sex chromosomes, meiosis during early spermatogenesis and oogenesis proceeds normally, without any disturbances, with the formation of haploid spermatocytes, and in female meiosis with the formation of synaptonemal complexes (SCs) and the lampbrush chromosomes. In females, the SC number was constantly equal to 19 (according to the SC length, 16 SC autosomal bivalents plus three presumed SC sex chromosome elements). No variability in the chromosomes at the early stages of meiotic prophase I, and no significant disturbances in the chromosome segregation at the anaphase–telophase I stage, have been discovered, and haploid oocytes (n = 17) at the metaphase II stage have been revealed. There should be a factor/factors that maintain the multiple sex chromosomes, their equal transmission, and the course of meiosis in these cryptic forms of Z. vivipara.
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13

Cox, R. M., and R. Calsbeek. "Cryptic Sex-Ratio Bias Provides Indirect Genetic Benefits Despite Sexual Conflict." Science 328, no. 5974 (March 4, 2010): 92–94. http://dx.doi.org/10.1126/science.1185550.

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14

Gibbs, H. Lisle, and Robert D. Denton. "Cryptic sex? Estimates of genome exchange in unisexual mole salamanders (Ambystomasp.)." Molecular Ecology 25, no. 12 (May 21, 2016): 2805–15. http://dx.doi.org/10.1111/mec.13662.

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15

Burt, A., D. A. Carter, G. L. Koenig, T. J. White, and J. W. Taylor. "Molecular markers reveal cryptic sex in the human pathogen Coccidioides immitis." Proceedings of the National Academy of Sciences 93, no. 2 (January 23, 1996): 770–73. http://dx.doi.org/10.1073/pnas.93.2.770.

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16

Alves, Fernanda, Germán M. López-Iborra, and Luís Fábio Silveira. "Population size assessment of the Endangered red-billed curassowCrax blumenbachii: accounting for variation in detectability and sex-biased estimates." Oryx 51, no. 1 (October 22, 2015): 137–45. http://dx.doi.org/10.1017/s0030605315000721.

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AbstractThe Endangered red-billed curassowCrax blumenbachiiis endemic to the lowland Atlantic Forest of Brazil and is extinct across most of its range as a result of habitat loss and hunting pressure. Global population estimates are unreliable as the species has never been surveyed systematically. During March 2012–February 2013 we used line transects to estimate the density and size of one of the most important populations, in the Vale Nature Reserve. UsingDistancewe estimated a density of 1.3 individuals per km2; results stratified by sex indicated a male-skewed sex ratio. Data collected from motion-activated cameras confirmed that the population was male skewed but to a lesser degree than suggested by transect data. Sex-specific behavioural responses to the presence of an observer probably contributed to the difference between camera and transect data. Using the camera-derived sex ratios we corrected transect estimates to 1.6 individuals per km2and a population of 325 individuals. Our systematic survey approach has revealed that Vale Nature Reserve has a larger population than previous estimates of the global population. We show that behavioural differences between the sexes in cryptic species can mislead population estimates, and we highlight the importance of motion-activated cameras as a tool for studying cryptic forest species.
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17

Tsubaki, Shin-ichi, Takanori Fukushima, Teruaki Tamagawa, Shin-ichiro Miyazaki, Kazuo Watanabe, Nobumasa Kuwana, and Tsuneo Shimizu. "Parapontine trigeminal cryptic angiomas presenting as trigeminal neuralgia." Journal of Neurosurgery 71, no. 3 (September 1989): 368–74. http://dx.doi.org/10.3171/jns.1989.71.3.0368.

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✓ Posterior fossa microvascular decompression surgery was attempted in 1257 patients with trigeminal neuralgia (TN), of whom seven had a very unusual cryptic angioma. The lesions were not visualized on preoperative enhanced computerized tomography scans, and serial angiography demonstrated a small vascular stain in only one case. The character of the facial pain was indistinguishable from TN caused by vascular compression and there was no other specific symptomatology. The patients' age and sex distributions were also compatible with classical TN. Cryptic angiomas presenting as typical TN without other symptoms have not been reported before, but they should be kept in mind in the differential diagnosis and surgical management of TN.
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18

Kawagoe, Tetsuhiro, and Nobuhiko Suzuki. "Cryptic dioecy in Actinidia polygama: a test of the pollinator attraction hypothesis." Canadian Journal of Botany 82, no. 2 (February 1, 2004): 214–18. http://dx.doi.org/10.1139/b03-150.

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Female flowers of some dioecious species are known to develop stamens with sterile pollen, a sexual system known as cryptic dioecy. A convincing hypothesis explaining this phenomenon is that stamens in female flowers attract pollinators and so ensure seed production. However, because this hypothesis has rarely been tested experimentally, we tested it in a cryptically dioecious species, Actinidia polygama (Sieb. et Zucc.) Planch. ex Maxim. Our findings show that pollinators do not show a consistent preference based on the sex of the flowers, and that the removal of stamens from female flowers results in a significant reduction in the frequency of insect visits and fruit production. These results strongly support the pollinator attraction hypothesis in A. polygama. Given the evident role of stamens in female flowers in A. polygama reproduction, we discuss why cryptic dioecy is a rare phenomenon.Key words: Actinidia polygama, cryptic dioecy, pseudopollen, pollinator attraction, unisexual flowers.
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19

Torres, María Del Milagro, Leonel Jeremías Viladrich, and Sergio Naretto. "Role of coloration in antipredator strategies of Pristidactylus achalensis (Squamata: Leiosauridae) related to sex and stages of predation." Biological Journal of the Linnean Society 133, no. 3 (April 20, 2021): 896–909. http://dx.doi.org/10.1093/biolinnean/blab012.

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Abstract The interaction between behavioural and phenotypic traits, such as coloration, plays a specific role at different stages of the predatory sequence. Cryptic coloration involves a match to the background that reduces the risk of detection, and it is usually optimized by immobility behaviour. In lizards, sexual dichromatism and within-individual changes of colour can influence the level of their crypsis and thus influence antipredator behaviour and the decision to flee. Here, we examined variation in coloration and antipredator behaviour between the sexes of the Achala copper lizard (Pristidactylus achalensis). We measured sexual dichromatism and crypsis in accordance with avian vision. We also exposed lizards to a raptor dummy to assess the colour change and behaviour at the detection and approach stages of the predatory event. Finally, we tested escape behaviour in the natural habitat. We found that males were more conspicuous than females. Additionally, individuals showed distinct behaviours at different stages of the predation simulation, but there were no differences between the sexes. However, in their natural habitat males initiated flight at greater distances than females, which might be related to their more conspicuous coloration. In summary, this study shows how differences in crypsis between the sexes affect escape behaviour during the approach stage of predation.
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Rahul, Aswathy, and Sobhakumar Saraswathyamma. "GENDER OUTCOME IN CHILDREN WITH DISORDERS OF SEX DEVELOPMENT: A CRYPTIC MISFORTUNE." Indian Journal of Child Health 04, no. 02 (June 25, 2017): 114–18. http://dx.doi.org/10.32677/ijch.2017.v04.i02.002.

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21

Chandler, C. H. "Cryptic intraspecific variation in sex determination in Caenorhabditis elegans revealed by mutations." Heredity 105, no. 5 (May 26, 2010): 473–82. http://dx.doi.org/10.1038/hdy.2010.62.

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22

Guerrero, R. F., M. Kirkpatrick, and N. Perrin. "Cryptic recombination in the ever‐young sex chromosomes of H ylid frogs." Journal of Evolutionary Biology 25, no. 10 (August 17, 2012): 1947–54. http://dx.doi.org/10.1111/j.1420-9101.2012.02591.x.

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23

Burgess, E. A., J. L. Brown, and J. M. Lanyon. "Sex, scarring, and stress: understanding seasonal costs in a cryptic marine mammal." Conservation Physiology 1, no. 1 (June 28, 2013): cot014. http://dx.doi.org/10.1093/conphys/cot014.

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24

van Velzen, Ellen, Lukas Schärer, and Ido Pen. "The effect of cryptic female choice on sex allocation in simultaneous hermaphrodites." Proceedings of the Royal Society B: Biological Sciences 276, no. 1670 (June 10, 2009): 3123–31. http://dx.doi.org/10.1098/rspb.2009.0566.

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25

Hall-Spencer, J. M., P. G. Moore, and L. U. Sneddon. "Observations and possible function of the striking anterior coloration pattern of Galathea intermedia (Crustacea: Decapoda: Anomura)." Journal of the Marine Biological Association of the United Kingdom 79, no. 2 (April 1999): 371–72. http://dx.doi.org/10.1017/s0025315498000459.

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Galathea intermedia is common, but cryptic, on Clyde maerl deposits where it lives in small groups of mixed sex and age, sharing shelters (typically dead Dosinia shells) to avoid predation. Its appearance is marked by six iridescent blue spots which may play an important role in intra- or interspecific interactions.
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26

Campera, Marco, Michela Balestri, Aria N. Stewart, and K. A. I. Nekaris. "Influence of Moon Luminosity, Seasonality, Sex and Weather Conditions on the Activity Levels of the Nocturnal Javan Slow Loris." Ecologies 3, no. 3 (June 27, 2022): 257–66. http://dx.doi.org/10.3390/ecologies3030020.

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The activity patterns of mammals depend on environmental changes (e.g., moon luminosity, food availability, weather) and endogenous rhythms. Behavioral observations are traditionally used to estimate the activity patterns of animals, but low visibility and the cryptic nature of some species entail that, in certain conditions, the animal is visible only for around 60% of the time. Recent advances in technology allow automatic data collection on the activity levels of animals. We used five years of data collected via accelerometers to understand how moon luminosity, seasonality, sex, and weather conditions influence the activity levels of the nocturnal and cryptic Javan slow loris. We collected 9589 h on six females and 7354 h on six males. Via Generalized Additive Mixed Models, we found that lorises are lunarphobic; they reduce activity levels during cold nights, they have higher activity levels when the relative humidity is close to 100%, and they have high peaks of activity between December and February and between June and August. The activity levels are thus influenced by avoidance of predators, food availability, consumption of insects and nectar, physiological, and behavioral adaptations to cold temperatures and energy requirements during reproductive stages. We highlight the importance of using bio-loggers for cryptic animals as with behavioral observations only, and the observer might underestimate active behaviors and overestimate inactivity.
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27

Couret, Jannelle, Danilo C. Moreira, Davin Bernier, Aria Mia Loberti, Ellen M. Dotson, and Marco Alvarez. "Delimiting cryptic morphological variation among human malaria vector species using convolutional neural networks." PLOS Neglected Tropical Diseases 14, no. 12 (December 17, 2020): e0008904. http://dx.doi.org/10.1371/journal.pntd.0008904.

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Deep learning is a powerful approach for distinguishing classes of images, and there is a growing interest in applying these methods to delimit species, particularly in the identification of mosquito vectors. Visual identification of mosquito species is the foundation of mosquito-borne disease surveillance and management, but can be hindered by cryptic morphological variation in mosquito vector species complexes such as the malaria-transmitting Anopheles gambiae complex. We sought to apply Convolutional Neural Networks (CNNs) to images of mosquitoes as a proof-of-concept to determine the feasibility of automatic classification of mosquito sex, genus, species, and strains using whole-body, 2D images of mosquitoes. We introduce a library of 1, 709 images of adult mosquitoes collected from 16 colonies of mosquito vector species and strains originating from five geographic regions, with 4 cryptic species not readily distinguishable morphologically even by trained medical entomologists. We present a methodology for image processing, data augmentation, and training and validation of a CNN. Our best CNN configuration achieved high prediction accuracies of 96.96% for species identification and 98.48% for sex. Our results demonstrate that CNNs can delimit species with cryptic morphological variation, 2 strains of a single species, and specimens from a single colony stored using two different methods. We present visualizations of the CNN feature space and predictions for interpretation of our results, and we further discuss applications of our findings for future applications in malaria mosquito surveillance.
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28

Sember, Alexandr, Michaela Pappová, Martin Forman, Petr Nguyen, František Marec, Martina Dalíková, Klára Divišová, et al. "Patterns of Sex Chromosome Differentiation in Spiders: Insights from Comparative Genomic Hybridisation." Genes 11, no. 8 (July 24, 2020): 849. http://dx.doi.org/10.3390/genes11080849.

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Spiders are an intriguing model to analyse sex chromosome evolution because of their peculiar multiple X chromosome systems. Y chromosomes were considered rare in this group, arising after neo-sex chromosome formation by X chromosome-autosome rearrangements. However, recent findings suggest that Y chromosomes are more common in spiders than previously thought. Besides neo-sex chromosomes, they are also involved in the ancient X1X2Y system of haplogyne spiders, whose origin is unknown. Furthermore, spiders seem to exhibit obligatorily one or two pairs of cryptic homomorphic XY chromosomes (further cryptic sex chromosome pairs, CSCPs), which could represent the ancestral spider sex chromosomes. Here, we analyse the molecular differentiation of particular types of spider Y chromosomes in a representative set of ten species by comparative genomic hybridisation (CGH). We found a high Y chromosome differentiation in haplogyne species with X1X2Y system except for Loxosceles spp. CSCP chromosomes exhibited generally low differentiation. Possible mechanisms and factors behind the observed patterns are discussed. The presence of autosomal regions marked predominantly or exclusively with the male or female probe was also recorded. We attribute this pattern to intraspecific variability in the copy number and distribution of certain repetitive DNAs in spider genomes, pointing thus to the limits of CGH in this arachnid group. In addition, we confirmed nonrandom association of chromosomes belonging to particular CSCPs at spermatogonial mitosis and spermatocyte meiosis and their association with multiple Xs throughout meiosis. Taken together, our data suggest diverse evolutionary pathways of molecular differentiation in different types of spider Y chromosomes.
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Lemos-Espinal, Julio A., and Geoffrey R. Smith. "Flight initiation distance of Urosaurus ornatus from the Sierra de Samalayuca, Mexico." Acta Herpetologica 16, no. 1 (April 11, 2021): 53–57. http://dx.doi.org/10.36253/a_h-9843.

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In lizards, flight initiation distance (FID), the distance between a prey individual and a predator when escape begins, can be affected by numerous intrinsic and extrinsic factors, including sex, temperature, and level of conspicuousness. Here we report on a study of FID in a population of Ornate Tree Lizards, Urosaurus ornatus, from the Sierra de Samalyuca, Chihuahua, Mexico which are cryptic due to their dorsal coloration blending into their background. Urosaurus ornatus in our study population allowed close approaches (mean FID = 65 cm). Mean FID did not differ between males and females. We also found no effect of body, air, or substrate temperature on FID. The short FID we observed may be related to the cryptic nature of U. ornatus.
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30

Chi, Jingyun, Matthew W. Parrow, and Micah Dunthorn. "Cryptic Sex inSymbiodinium(Alveolata, Dinoflagellata) is Supported by an Inventory of Meiotic Genes." Journal of Eukaryotic Microbiology 61, no. 3 (April 29, 2014): 322–27. http://dx.doi.org/10.1111/jeu.12110.

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31

Thompson, Stacey Lee, Gina Choe, Kermit Ritland, and Jeannette Whitton. "Cryptic Sex within Male‐Sterile Polyploid Populations of the Easter Daisy,Townsendia hookeri." International Journal of Plant Sciences 169, no. 1 (January 2008): 183–93. http://dx.doi.org/10.1086/523363.

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32

Mikheyev, A. S., U. G. Mueller, and P. Abbot. "Cryptic sex and many-to-one coevolution in the fungus-growing ant symbiosis." Proceedings of the National Academy of Sciences 103, no. 28 (June 30, 2006): 10702–6. http://dx.doi.org/10.1073/pnas.0601441103.

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33

Igarashi, Maki, Vu Chi Dung, Erina Suzuki, Shinobu Ida, Mariko Nakacho, Kazuhiko Nakabayashi, Kentaro Mizuno, et al. "Cryptic Genomic Rearrangements in Three Patients with 46,XY Disorders of Sex Development." PLoS ONE 8, no. 7 (July 8, 2013): e68194. http://dx.doi.org/10.1371/journal.pone.0068194.

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34

Edwards, A. M., and E. Z. Cameron. "Cryptic male choice: experimental evidence of sperm sex ratio and seminal fluid adjustment in relation to coital rate." Reproduction, Fertility and Development 29, no. 7 (2017): 1401. http://dx.doi.org/10.1071/rd16123.

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The differential allocation hypothesis suggests that a mother should adjust the sex of offspring in relation to her mate’s attractiveness, thereby increasing future reproductive fitness when her sons inherit the attractive traits. More attractive males have been shown to sire more sons, but it is possible that the sex ratio skew could be a result of paternal rather than maternal manipulation, which would be a more parsimonious explanation. We manipulated coital rate (an indicator of attractiveness) in laboratory mice and showed that males that mate more often have higher levels of glucose in their semen despite lower blood glucose levels. Since peri-conceptual glucose levels in utero increase male conceptus survival, this could result in male-biased sex ratios. The males that mated most also had more remaining X-chromosome-bearing-spermatozoa, suggesting depletion of Y-chromosome-bearing-spermatozoa during mating. We hypothesise that males may alter both seminal fluids and X : Y ratios in an ejaculate to influence subsequent sex ratios. Our results further support a paternal role in sex allocation.
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35

Yang, Qiong, Jessica Chung, Katie L. Robinson, Thomas L. Schmidt, Perran A. Ross, Jiaxin Liang, and Ary A. Hoffmann. "Sex-specific distribution and classification of Wolbachia infections and mitochondrial DNA haplogroups in Aedes albopictus from the Indo-Pacific." PLOS Neglected Tropical Diseases 16, no. 4 (April 13, 2022): e0010139. http://dx.doi.org/10.1371/journal.pntd.0010139.

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The arbovirus vector Aedes albopictus (Asian tiger mosquito) is common throughout the Indo-Pacific region, where most global dengue transmission occurs. We analysed population genomic data and tested for cryptic species in 160 Ae. albopictus sampled from 16 locations across this region. We found no evidence of cryptic Ae. albopictus but found multiple intraspecific COI haplotypes partitioned into groups representing three Asian lineages: East Asia, Southeast Asia and Indonesia. Papua New Guinea (PNG), Vanuatu and Christmas Island shared recent coancestry, and Indonesia and Timor-Leste were likely invaded from East Asia. We used a machine learning trained on morphologically sexed samples to classify sexes using multiple genetic features and then characterized the wAlbA and wAlbB Wolbachia infections in 664 other samples. The wAlbA and wAlbB infections as detected by qPCR showed markedly different patterns in the sexes. For females, most populations had a very high double infection incidence, with 67% being the lowest value (from Timor-Leste). For males, the incidence of double infections ranged from 100% (PNG) to 0% (Vanuatu). Only 6 females were infected solely by the wAlbA infection, while rare uninfected mosquitoes were found in both sexes. The wAlbA and wAlbB densities varied significantly among populations. For mosquitoes from Torres Strait and Vietnam, the wAlbB density was similar in single-infected and superinfected (wAlbA and wAlbB) mosquitoes. There was a positive association between wAlbA and wAlbB infection densities in superinfected Ae. albopictus. Our findings provide no evidence of cryptic species of Ae. albopictus in the region and suggest site-specific factors influencing the incidence of Wolbachia infections and their densities. We also demonstrate the usefulness of ddRAD tag depths as sex-specific mosquito markers. The results provide baseline data for the exploitation of Wolbachia-induced cytoplasmic incompatibility (CI) in dengue control.
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Panthum, Thitipong, Nararat Laopichienpong, Ekaphan Kraichak, Worapong Singchat, Dung Ho My Nguyen, Nattakan Ariyaraphong, Syed Farhan Ahmad, et al. "The Snakeskin Gourami (Trichopodus pectoralis) Tends to Exhibit XX/XY Sex Determination." Fishes 6, no. 4 (September 23, 2021): 43. http://dx.doi.org/10.3390/fishes6040043.

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The snakeskin gourami (Trichopodus pectoralis) has a high meat yield and is one of the top five aquaculture freshwater fishes in Thailand. The species is not externally sexually dimorphic, and its sex determination system is unknown. Understanding the sex determination system of this species will contribute to its full-scale commercialization. In this study, a cytogenetic analysis did not reveal any between-sex differences in chromosomal patterns. However, we used genotyping-by-sequencing to identify 4 male-linked loci and 1 female-linked locus, indicating that the snakeskin gourami tends to exhibit an XX/XY sex determination system. However, we did not find any male-specific loci after filtering the loci for a ratio of 100:0 ratio of males:females. This suggests that the putative Y chromosome is young and that the sex determination region is cryptic. This approach provides solid information that can help identify the sex determination mechanism and potential sex determination regions in the snakeskin gourami, allowing further investigation of genetic improvements in the species.
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STÖCK, M., D. CROLL, Z. DUMAS, S. BIOLLAY, J. WANG, and N. PERRIN. "A cryptic heterogametic transition revealed by sex-linked DNA markers in Palearctic green toads." Journal of Evolutionary Biology 24, no. 5 (February 21, 2011): 1064–70. http://dx.doi.org/10.1111/j.1420-9101.2011.02239.x.

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38

Groenewald, Marizeth, Johannes Z. Groenewald, Thomas C. Harrington, Edwin C. A. Abeln, and Pedro W. Crous. "Mating type gene analysis in apparently asexual Cercospora species is suggestive of cryptic sex." Fungal Genetics and Biology 43, no. 12 (December 2006): 813–25. http://dx.doi.org/10.1016/j.fgb.2006.05.008.

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39

Lee, Gilho, and Inho Sohng. "Cryptic Plasmid Amplification ofChlamydia trachomatisat a Korean Health Center for Female Commercial Sex Workers." Korean Journal of Urology 47, no. 1 (2006): 37. http://dx.doi.org/10.4111/kju.2006.47.1.37.

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40

Vanthournout, B., K. Deswarte, H. Hammad, T. Bilde, B. Lambrecht, and F. Hendrickx. "Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species." Biology Letters 10, no. 5 (May 2014): 20140159. http://dx.doi.org/10.1098/rsbl.2014.0159.

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Producing equal amounts of male and female offspring has long been considered an evolutionarily stable strategy. Nevertheless, exceptions to this general rule (i.e. male and female biases) are documented in many taxa, making sex allocation an important domain in current evolutionary biology research. Pinpointing the underlying mechanism of sex ratio bias is challenging owing to the multitude of potential sex ratio-biasing factors. In the dwarf spider, Oedothorax gibbosus , infection with the bacterial endosymbiont Wolbachia results in a female bias. However, pedigree analysis reveals that other factors influence sex ratio variation. In this paper, we investigate whether this additional variation can be explained by the unequal production of male- and female-determining sperm cells during sperm production. Using flow cytometry, we show that males produce equal amounts of male- and female-determining sperm cells; thus bias in sperm production does not contribute to the sex ratio bias observed in this species. This demonstrates that other factors such as parental genes suppressing endosymbiont effects and cryptic female choice might play a role in sex allocation in this species.
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Rovatsos, Michail T., Juan A. Marchal, Ismael Romero-Fernández, Maria Arroyo, Eva B. Athanasopoulou, and Antonio Sánchez. "Extensive Sex Chromosome Polymorphism of Microtus thomasi/Microtus atticus Species Complex Associated with Cryptic Chromosomal Rearrangements and Independent Accumulation of Heterochromatin." Cytogenetic and Genome Research 151, no. 4 (2017): 198–207. http://dx.doi.org/10.1159/000477114.

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The sibling species Microtus thomasi and M. atticus represent probably the highest karyotypic diversity within the genus Microtus and are an interesting model for chromosomal evolution studies. In addition to variation in autosomes, they show a high intraspecific variation in the size and morphology of both sex chromosomes. We analyzed individuals with different sex chromosome constitutions using 3 painting probes, 2 from Y chromosome variants and 1 from the small arm of the submetacentric X chromosome. Our comparative painting approach uncovered 12 variants of Y and 14 variants of X chromosomes, which demonstrates that the polymorphism of sex chromosomes is substantially larger than previously reported. We suggest that 2 main processes are responsible for this sex chromosome polymorphism: change of morphology from acrocentric to submetacentric or metacentric chromosomes and increase in size due to accumulation of repetitive DNA sequences, generating heterochromatic blocks. Strong genetic drift in small and fragmented populations of these 2 species could be related to the origin and maintenance of the large polymorphism of sex chromosomes. We proposed that a similar polymorphism variation combined with random drift fixing the biggest sex chromosomes could have occurred in the origin of some of the actual Microtus species with giant sex chromosomes.
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42

Bower, Colin C. "Specific pollinators reveal a cryptic taxon in the bird orchid, Chiloglottis valida sensu lato (Orchidaceae) in south-eastern Australia." Australian Journal of Botany 54, no. 1 (2006): 53. http://dx.doi.org/10.1071/bt05043.

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Orchid species belonging to the sexual-deception pollination syndrome exhibit highly specific, usually one-to-one, relationships with their pollinators. This specificity is mediated by the orchid’s mimicry of the sex-attractant pheromones emitted by females of the pollinator species. Chiloglottis valida D.Jones sensu lato is a widespread, sexually deceptive, terrestrial orchid found in south-eastern New South Wales, and eastern and southern Victoria from sea level to at least 1600 m in the Australian Alps. Flowers from 38 C. valida s.l. populations from throughout this area were compared in field choice experiments for the specificity of attracted pollinator species. Four potential pollinator wasps in the thynnine genus Neozeleboria Rohwer were attracted. The data demonstrate the existence of two attractant odour types among C. valida s.l. and its pollinators, and support the recognition of two partially sympatric cryptic species in the orchid, each with two potential pollinators. The copheromone pollinator pairs replace each other on the altitudinal gradient, albeit with some overlap. In alpine areas the pollinators of the two cryptic orchid species are themselves sibling species within Neozeleboria monticola Turner s.l. The results indicate that C. aff. valida, the sister species of C. valida s.s., has two geographically replacing pollinators.
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Miyawaki, Shingo, Shunsuke Kuroki, Ryo Maeda, Naoki Okashita, Peter Koopman, and Makoto Tachibana. "The mouse Sry locus harbors a cryptic exon that is essential for male sex determination." Science 370, no. 6512 (October 1, 2020): 121–24. http://dx.doi.org/10.1126/science.abb6430.

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The mammalian sex-determining gene Sry induces male development. Since its discovery 30 years ago, Sry has been believed to be a single-exon gene. Here, we identified a cryptic second exon of mouse Sry and a corresponding two-exon type Sry (Sry-T) transcript. XY mice lacking Sry-T were sex-reversed, and ectopic expression of Sry-T in XX mice induced male development. Sry-T messenger RNA is expressed similarly to that of canonical single-exon type Sry (Sry-S), but SRY-T protein is expressed predominantly because of the absence of a degron in the C terminus of SRY-S. Sry exon2 appears to have evolved recently in mice through acquisition of a retrotransposon-derived coding sequence to replace the degron. Our findings suggest that in nature, SRY-T, not SRY-S, is the bona fide testis-determining factor.
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Rebijith, K. B., R. Asokan, N. K. Krishna Kumar, V. Krishna, B. N. Chaitanya, and V. V. Ramamurthy. "DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India." Bulletin of Entomological Research 103, no. 5 (May 17, 2013): 601–10. http://dx.doi.org/10.1017/s0007485313000278.

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AbstractRapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol.
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Sakanoue, Rei, and Yoichi Sakai. "Cryptic bachelor sex change in haremic colonial groups of the coral-dwelling damselfish Dascyllus reticulatus." Journal of Ethology 40, no. 2 (March 18, 2022): 181–92. http://dx.doi.org/10.1007/s10164-022-00748-z.

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46

Jackson, Tim P., and Rudi J. van Aarde. "SEX- AND SPECIES-SPECIFIC GROWTH PATTERNS IN CRYPTIC AFRICAN RODENTS, MASTOMYS NATALENSIS AND M. COUCHA." Journal of Mammalogy 84, no. 3 (August 2003): 851–60. http://dx.doi.org/10.1644/bpr-001.

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47

Blanc, Guillaume, Garry Duncan, Irina Agarkova, Mark Borodovsky, James Gurnon, Alan Kuo, Erika Lindquist, et al. "The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex." Plant Cell 22, no. 9 (September 2010): 2943–55. http://dx.doi.org/10.1105/tpc.110.076406.

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48

Krebes, Lukas, Lisza Zeidler, Jens Frankowski, and Ralf Bastrop. "(Cryptic) sex in the microsporidian Nosema granulosis – Evidence from parasite rDNA and host mitochondrial DNA." Infection, Genetics and Evolution 21 (January 2014): 259–68. http://dx.doi.org/10.1016/j.meegid.2013.11.007.

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49

Cole, Kathleen S., and Douglas Y. Shapiro. "Social faciliation and sensory mediation of adult sex change in a cryptic, benthic marine goby." Journal of Experimental Marine Biology and Ecology 186, no. 1 (March 1995): 65–75. http://dx.doi.org/10.1016/0022-0981(94)00152-4.

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50

Iannucci, Alessio, Marta Svartman, Massimo Bellavita, Guido Chelazzi, Roscoe Stanyon, and Claudio Ciofi. "Insights into Emydid Turtle Cytogenetics: The European Pond Turtle as a Model Species." Cytogenetic and Genome Research 157, no. 3 (2019): 166–71. http://dx.doi.org/10.1159/000495833.

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Our knowledge of Testudines evolution is limited by the lack of modern cytogenetic data. Compared to other reptiles, there is little information even on chromosome banding, let alone molecular cytogenetic data. Here, we provide detailed information on the karyotype of the European pond turtle Emys orbicularis, a model Emydidae, employing both chromosome banding and molecular cytogenetics. We provide a high-resolution G-banded karyotype and a map of rDNA genes and telomeric sequences using fluorescence in situ hybridization. We test hypotheses of sex-determining mechanisms in Emys by comparative genomic hybridization to determine if Emys has a cryptic sex-specific region. Our results provide valuable data to guide future efforts on genome sequencing and anchoring in Emydidae and for understanding karyotype evolution in Testudines.
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